##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3452456 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894673820607707 33.0 31.0 34.0 30.0 34.0 2 32.0908107156181 34.0 31.0 34.0 30.0 34.0 3 32.16025750943676 34.0 31.0 34.0 30.0 34.0 4 35.68144532471956 37.0 35.0 37.0 35.0 37.0 5 35.702871231378474 37.0 35.0 37.0 35.0 37.0 6 35.732471318968294 37.0 36.0 37.0 35.0 37.0 7 35.67301045980021 37.0 35.0 37.0 33.0 37.0 8 35.6592509795925 37.0 35.0 37.0 33.0 37.0 9 37.32649250272849 39.0 37.0 39.0 34.0 39.0 10-11 37.24291663673628 39.0 37.0 39.0 34.0 39.0 12-13 37.14477896314971 39.0 37.0 39.0 34.0 39.0 14-15 38.40728209135757 40.0 38.0 41.0 34.0 41.0 16-17 38.33083390490712 40.0 38.0 41.0 33.5 41.0 18-19 38.277486374916876 40.0 38.0 41.0 33.5 41.0 20-21 38.170888202485415 40.0 38.0 41.0 33.5 41.0 22-23 38.022800435400185 40.0 38.0 41.0 33.0 41.0 24-25 37.98087231234808 40.0 38.0 41.0 33.0 41.0 26-27 37.98904547950792 40.0 38.0 41.0 33.0 41.0 28-29 37.90381513913574 40.0 38.0 41.0 33.0 41.0 30-31 37.72524095889998 40.0 37.5 41.0 33.0 41.0 32-33 37.505516218019864 40.0 37.0 41.0 32.0 41.0 34-35 37.29883277295931 40.0 37.0 41.0 31.5 41.0 36-37 37.07880940408799 40.0 36.5 41.0 31.0 41.0 38-39 36.842363957715904 40.0 36.0 41.0 30.0 41.0 40-41 36.62692645467459 39.5 36.0 41.0 30.0 41.0 42-43 36.34931176530563 39.0 35.5 41.0 30.0 41.0 44-45 36.12033201871363 39.0 35.0 41.0 29.0 41.0 46-47 35.97481054067018 39.0 35.0 41.0 28.5 41.0 48-49 35.73040148810007 39.0 35.0 41.0 27.5 41.0 50-51 35.096173854206974 38.5 34.5 40.5 26.5 40.5 52-53 35.16972627601916 38.5 35.0 40.0 26.5 41.0 54-55 35.312072767907836 39.0 35.0 40.5 26.5 41.0 56-57 35.11050032208955 39.0 35.0 40.5 26.0 41.0 58-59 34.86394859195889 38.5 35.0 40.0 26.0 41.0 60-61 34.50933436950391 38.0 34.0 40.0 24.5 41.0 62-63 34.10331963680348 37.5 34.0 40.0 23.0 41.0 64-65 33.687600073686674 37.0 33.5 40.0 22.0 41.0 66-67 33.26964644878892 36.5 33.0 39.5 21.5 41.0 68-69 32.796653599640365 36.0 32.5 39.0 19.5 41.0 70-71 32.325570695180474 35.0 32.0 39.0 18.0 40.0 72-73 31.83726222723765 35.0 31.5 38.0 14.5 40.0 74-75 31.309369474947687 35.0 31.0 37.0 9.0 39.0 76-77 30.765941984488723 35.0 30.5 36.5 7.0 39.0 78-79 30.192050963140446 34.5 30.0 36.0 2.0 38.5 80-81 29.663782246609372 34.0 29.5 35.5 2.0 37.5 82-83 29.125256628904175 34.0 29.0 35.0 2.0 37.0 84-85 28.626743251760487 34.0 29.0 35.0 2.0 36.5 86-87 28.124142494502465 34.0 27.5 35.0 2.0 36.0 88-89 27.57322091867355 33.5 26.5 35.0 2.0 35.5 90-91 26.965592175541122 33.0 25.5 35.0 2.0 35.0 92-93 26.354794818529186 32.5 24.5 35.0 2.0 35.0 94-95 25.752984542018783 32.0 23.5 35.0 2.0 35.0 96-97 24.913149074166334 32.0 18.5 34.0 2.0 35.0 98-99 23.830335564015876 31.5 2.0 34.0 2.0 35.0 100-101 20.904164455680245 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44747245058659146 1101 2 0.40445537613507554 1101 3 0.3528041987494248 1101 4 0.20691696455419617 1101 5 0.2743229023872189 1101 6 0.2679843482602635 1101 7 0.31061795718181884 1101 8 0.2997125303764605 1101 9 0.31789577517529466 1101 10-11 0.36809834309357825 1101 12-13 0.21913979236803272 1101 14-15 0.4286994134393609 1101 16-17 0.4796323039567767 1101 18-19 0.45524169666087033 1101 20-21 0.5359703606650115 1101 22-23 0.5074696334835949 1101 24-25 0.5073707169103443 1101 26-27 0.5733942412929238 1101 28-29 0.5284515264333223 1101 30-31 0.5107941400739548 1101 32-33 0.635755800066029 1101 34-35 0.5766347947780588 1101 36-37 0.6326776468115582 1101 38-39 0.748029059111559 1101 40-41 0.7111366546415283 1101 42-43 0.6249027481376288 1101 44-45 0.6556981572541432 1101 46-47 0.7890735065723717 1101 48-49 0.7685280760731388 1101 50-51 0.8532523820299005 1101 52-53 0.8623733448710311 1101 54-55 0.7756668065942236 1101 56-57 0.8249643716818866 1101 58-59 0.826067992782086 1101 60-61 0.822299998119 1101 62-63 0.7800551262954585 1101 64-65 0.7240768516063767 1101 66-67 0.6532766811002162 1101 68-69 0.7911540184516213 1101 70-71 1.0192326334300716 1101 72-73 1.0437966068288773 1101 74-75 1.0167530904658655 1101 76-77 1.009144983076407 1101 78-79 1.0330930039735584 1101 80-81 1.096723852916064 1101 82-83 1.0369641530868492 1101 84-85 1.0894469541510539 1101 86-87 1.100944396809961 1101 88-89 1.1745350096174825 1101 90-91 1.2192633798190684 1101 92-93 1.3083286318589522 1101 94-95 1.4865343060045326 1101 96-97 1.3856662688015504 1101 98-99 1.6734924077027777 1101 100-101 1.715738368904475 1102 1 0.4165071921858505 1102 2 0.25921247643989176 1102 3 0.2830601238476973 1102 4 0.2004935545031401 1102 5 0.19004520321378493 1102 6 0.2314755456554991 1102 7 0.12913555002629806 1102 8 0.3048670225488479 1102 9 0.3705233496721263 1102 10-11 0.3987391634598296 1102 12-13 0.3487122192392462 1102 14-15 0.32261064917153703 1102 16-17 0.25564088477978686 1102 18-19 0.38991368328346 1102 20-21 0.3785595087757585 1102 22-23 0.4519852573901417 1102 24-25 0.35011466500446886 1102 26-27 0.3092760566094981 1102 28-29 0.32191204270336726 1102 30-31 0.3673298090524497 1102 32-33 0.3509069405188683 1102 34-35 0.40330380115895537 1102 36-37 0.46365616476219884 1102 38-39 0.4968809767739657 1102 40-41 0.49313234318312027 1102 42-43 0.5219564445791747 1102 44-45 0.4002036260292172 1102 46-47 0.39323568980987744 1102 48-49 0.4962173837720485 1102 50-51 0.4772797387965042 1102 52-53 0.4647068151846341 1102 54-55 0.3979273283364577 1102 56-57 0.43648202714090445 1102 58-59 0.5139247180836719 1102 60-61 0.5758694742141941 1102 62-63 0.5587185636653871 1102 64-65 0.5199622869905411 1102 66-67 0.5215214797523728 1102 68-69 0.5090689331827178 1102 70-71 0.6488539943544964 1102 72-73 0.6737419848496984 1102 74-75 0.5875940774183626 1102 76-77 0.43071232501546675 1102 78-79 0.4674482872990957 1102 80-81 0.4802628057378513 1102 82-83 0.5172971887818747 1102 84-85 0.5087405463683261 1102 86-87 0.4142878186262351 1102 88-89 0.4183597162630974 1102 90-91 0.6298818588960735 1102 92-93 0.6636302682031676 1102 94-95 0.6416452573930265 1102 96-97 0.600873551872418 1102 98-99 1.0395421720563718 1102 100-101 0.9331566293041469 1103 1 0.43794628878035624 1103 2 0.39096006511908143 1103 3 0.3163225536455485 1103 4 0.2423164384916916 1103 5 0.2524569337421738 1103 6 0.21178325284841293 1103 7 0.28605496336165714 1103 8 0.32393890530675407 1103 9 0.3259695359619954 1103 10-11 0.3428384310422672 1103 12-13 0.30268646195443694 1103 14-15 0.4187149203156082 1103 16-17 0.3991472875714308 1103 18-19 0.3462115511916011 1103 20-21 0.3574197570681221 1103 22-23 0.38428468915812175 1103 24-25 0.5433774798435635 1103 26-27 0.42925846318640026 1103 28-29 0.3967184484075048 1103 30-31 0.36515605375998916 1103 32-33 0.5418597547014201 1103 34-35 0.5502570635690063 1103 36-37 0.5216741303918369 1103 38-39 0.42379886475361417 1103 40-41 0.5458407381189403 1103 42-43 0.5249511275544805 1103 44-45 0.5198059771503054 1103 46-47 0.45154890309262186 1103 48-49 0.408182392408321 1103 50-51 0.5192683879780233 1103 52-53 0.6130856681270203 1103 54-55 0.6085607359959155 1103 56-57 0.5145327508950359 1103 58-59 0.5501016139305079 1103 60-61 0.4747381934139767 1103 62-63 0.5368655477311037 1103 64-65 0.6657804181879143 1103 66-67 0.668265388325807 1103 68-69 0.6830728734381424 1103 70-71 0.6867265032486998 1103 72-73 0.7843034194146554 1103 74-75 0.7193182180729458 1103 76-77 0.6813161239903671 1103 78-79 0.7652197376754266 1103 80-81 0.8741327022752934 1103 82-83 0.8948613290020511 1103 84-85 1.0010699817349575 1103 86-87 0.9103585220719097 1103 88-89 0.8539368363224149 1103 90-91 0.9621835614201224 1103 92-93 0.9604470137456964 1103 94-95 1.015857070557189 1103 96-97 1.1875428809183042 1103 98-99 1.1591066607882397 1103 100-101 1.0328407473893861 1104 1 0.3954349761418605 1104 2 0.3202048650297016 1104 3 0.3074726253388107 1104 4 0.26817815428842096 1104 5 0.2000384499150556 1104 6 0.2351966902145719 1104 7 0.20891394177918698 1104 8 0.2651886027053152 1104 9 0.15746475951463168 1104 10-11 0.3261457716876919 1104 12-13 0.22403126931371276 1104 14-15 0.2993689118613858 1104 16-17 0.4493835494659919 1104 18-19 0.4330043949002871 1104 20-21 0.3885769980878493 1104 22-23 0.4040194240792516 1104 24-25 0.4864367338611757 1104 26-27 0.4720281509017141 1104 28-29 0.4811328447874601 1104 30-31 0.42684508189726955 1104 32-33 0.4762643747271156 1104 34-35 0.49657137801379747 1104 36-37 0.5693343229394614 1104 38-39 0.5579430147694424 1104 40-41 0.5666920723585207 1104 42-43 0.6455648032774661 1104 44-45 0.6057445537131301 1104 46-47 0.7833178645644736 1104 48-49 0.7413824259272559 1104 50-51 0.7128986276383671 1104 52-53 0.6369222819703353 1104 54-55 0.7564911749076728 1104 56-57 0.7163835078641796 1104 58-59 0.8017696461831605 1104 60-61 0.8036203371356905 1104 62-63 0.8026082713302856 1104 64-65 0.8459814386530837 1104 66-67 0.8291527554139435 1104 68-69 0.9480318593043293 1104 70-71 0.9971809175054247 1104 72-73 1.1356509269098538 1104 74-75 1.017551258426007 1104 76-77 1.0538324644681971 1104 78-79 0.9800320782277403 1104 80-81 1.1960107864822902 1104 82-83 1.041191937370609 1104 84-85 1.0701301482123036 1104 86-87 1.071199800632833 1104 88-89 1.1512993156058542 1104 90-91 1.2357864738426052 1104 92-93 1.2996567118891207 1104 94-95 1.4391131551738638 1104 96-97 1.5039722770189314 1104 98-99 1.6533933128058926 1104 100-101 1.328694642102704 1105 1 0.43014685342331305 1105 2 0.3788715809300811 1105 3 0.30691382270269685 1105 4 0.26360152904840106 1105 5 0.2131773452512178 1105 6 0.26173665040560223 1105 7 0.17375227936772575 1105 8 0.25874285473972236 1105 9 0.3337603094903656 1105 10-11 0.3588435505439236 1105 12-13 0.28015691023114186 1105 14-15 0.4106736755747278 1105 16-17 0.35319872259282903 1105 18-19 0.41363910537089055 1105 20-21 0.46706204158935094 1105 22-23 0.467225488265953 1105 24-25 0.4763665054870856 1105 26-27 0.3778080129066481 1105 28-29 0.47225451184096556 1105 30-31 0.465192956759239 1105 32-33 0.448719607215736 1105 34-35 0.609675809751252 1105 36-37 0.5949555468895582 1105 38-39 0.6455992438890803 1105 40-41 0.735074445986136 1105 42-43 0.743271391451529 1105 44-45 0.820269114302242 1105 46-47 0.8212403074006147 1105 48-49 0.7666704431449887 1105 50-51 0.7120478515040531 1105 52-53 0.5836113148092252 1105 54-55 0.5665760854781468 1105 56-57 0.7866065189980276 1105 58-59 0.7842907990960413 1105 60-61 0.7424376026953041 1105 62-63 0.6661426188780979 1105 64-65 0.7185999336488251 1105 66-67 0.7128016240935651 1105 68-69 0.8295643816880798 1105 70-71 0.8922477874369861 1105 72-73 0.9100304942158601 1105 74-75 0.7602784720088884 1105 76-77 0.7511731406580715 1105 78-79 0.8677910827220785 1105 80-81 0.9877013666664247 1105 82-83 1.0226322344608967 1105 84-85 0.9550428287066133 1105 86-87 0.9563001846557917 1105 88-89 0.9655641683618867 1105 90-91 0.9597611817811931 1105 92-93 1.1191251284625956 1105 94-95 1.1701397132450282 1105 96-97 1.2437058171338577 1105 98-99 1.1989801259076849 1105 100-101 0.9723255580607599 1106 1 0.15977966102729013 1106 2 0.13257882958406952 1106 3 0.09025810384098776 1106 4 0.09559765422959288 1106 5 0.11682408758348117 1106 6 0.14944570234580823 1106 7 0.2646584591940524 1106 8 0.1303564538917925 1106 9 0.17381338203457375 1106 10-11 -0.3894626412864284 1106 12-13 -0.11392131644575443 1106 14-15 0.004357043595675236 1106 16-17 0.16779865157459994 1106 18-19 0.21373394722791517 1106 20-21 0.1955306599495401 1106 22-23 0.041272802901843875 1106 24-25 0.07452019925636222 1106 26-27 0.113981086134892 1106 28-29 0.0638694488718059 1106 30-31 0.15163600319301906 1106 32-33 0.2236371801359951 1106 34-35 -0.0012900850700106048 1106 36-37 -0.1496379475324332 1106 38-39 0.12186815819207908 1106 40-41 0.2355151244750857 1106 42-43 0.11877833517645797 1106 44-45 0.28806144332788364 1106 46-47 0.5095254519502035 1106 48-49 0.4724553102272324 1106 50-51 0.4695793712907488 1106 52-53 0.49079278984906693 1106 54-55 0.14567556900329492 1106 56-57 0.18211458265155045 1106 58-59 0.3364358814862385 1106 60-61 0.052882982201886364 1106 62-63 0.29937581248859857 1106 64-65 0.19443043279160577 1106 66-67 0.001268991755061677 1106 68-69 -0.27073167847689916 1106 70-71 -0.0012941141548239443 1106 72-73 0.25329355370652706 1106 74-75 0.33371502319213775 1106 76-77 0.3550729231437977 1106 78-79 0.5105932949498886 1106 80-81 0.42437057832052005 1106 82-83 0.39123242095918087 1106 84-85 0.4845990519720864 1106 86-87 0.30773251967672977 1106 88-89 0.18280843652375722 1106 90-91 0.23759266471753548 1106 92-93 0.4224060665737994 1106 94-95 0.26264363550991376 1106 96-97 0.4224966329146582 1106 98-99 0.3497216762308746 1106 100-101 0.10848364302310287 1107 1 0.27064440109930743 1107 2 0.16262301408019653 1107 3 0.1692371349006052 1107 4 0.17484971588105225 1107 5 0.17203975152037287 1107 6 0.17440254410103506 1107 7 0.0864278756309389 1107 8 0.17694810609282285 1107 9 0.17172985313008837 1107 10-11 0.21096663408464877 1107 12-13 0.0803686687730405 1107 14-15 0.19210328425400292 1107 16-17 0.17850738553047307 1107 18-19 0.23543948150710037 1107 20-21 0.22921628654361115 1107 22-23 0.19853349142898935 1107 24-25 0.2933327389341045 1107 26-27 0.3345811329128523 1107 28-29 0.3705360253768575 1107 30-31 0.3467116673526647 1107 32-33 0.451550978204736 1107 34-35 0.4582783165713522 1107 36-37 0.3608038019635913 1107 38-39 0.4191042803303091 1107 40-41 0.5109862852210512 1107 42-43 0.6026243433769167 1107 44-45 0.46888868147069473 1107 46-47 0.5008803583067518 1107 48-49 0.4521665596922304 1107 50-51 0.4086513059416035 1107 52-53 0.5191687192429768 1107 54-55 0.5309117102345695 1107 56-57 0.5259281963132665 1107 58-59 0.459957244220746 1107 60-61 0.4109310203116152 1107 62-63 0.505244856451931 1107 64-65 0.47573730422061544 1107 66-67 0.4539253916653223 1107 68-69 0.5923736308225287 1107 70-71 0.6595332353641155 1107 72-73 0.6672352976337521 1107 74-75 0.5436872428067439 1107 76-77 0.549844212290516 1107 78-79 0.5149797637064992 1107 80-81 0.6163950937946439 1107 82-83 0.5689011120193399 1107 84-85 0.6345062120243377 1107 86-87 0.7100322773541201 1107 88-89 0.578928808647273 1107 90-91 0.7554640762188747 1107 92-93 0.7617866721734714 1107 94-95 0.5672219894027464 1107 96-97 0.6551478936127317 1107 98-99 0.705283560778394 1107 100-101 0.803740532033622 1108 1 0.29084403035703943 1108 2 0.2651987176479125 1108 3 0.20851818689538248 1108 4 0.18147710281591856 1108 5 0.17079084553623147 1108 6 0.16711637906247745 1108 7 0.22448759045294508 1108 8 0.19607092087623101 1108 9 0.23731139584970862 1108 10-11 0.2852853155654742 1108 12-13 0.1492624294462459 1108 14-15 0.20592465544125105 1108 16-17 0.31622843109511933 1108 18-19 0.282394681023753 1108 20-21 0.33912544925163246 1108 22-23 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-1.2145386446575372 2316 88-89 -1.458039510639093 2316 90-91 -1.3809298533172658 2316 92-93 -1.2270420948182128 2316 94-95 -1.2441430699178433 2316 96-97 -1.3712254044027574 2316 98-99 -1.5119529682763435 2316 100-101 -1.4753764137062753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9156.0 3 2462.0 4 2498.0 5 2851.0 6 3232.0 7 3918.0 8 4770.0 9 6072.0 10 7933.0 11 9741.0 12 12514.0 13 15563.0 14 18297.0 15 22416.0 16 27035.0 17 35169.0 18 31928.0 19 24890.0 20 24692.0 21 25001.0 22 26914.0 23 29396.0 24 33018.0 25 36784.0 26 42296.0 27 49082.0 28 56944.0 29 67831.0 30 81639.0 31 99682.0 32 124233.0 33 160843.0 34 220918.0 35 324390.0 36 494289.0 37 699607.0 38 551749.0 39 62699.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7887263263916 18.96590068873972 14.905451115781629 23.339921869087053 2 15.167571295681833 19.89135758106162 40.85212865534359 24.088942467912958 3 18.75613857997347 24.177999039563105 29.967109238539315 27.098753141924114 4 12.556384330786141 16.85894026586826 37.699093242285606 32.88558216105999 5 13.537535514441599 37.20403182580972 34.580608542366434 14.67782411738224 6 31.61959109348133 36.63909113842567 17.465661073270873 14.275656694822123 7 28.127309775913695 32.90161477792818 20.868780090492493 18.102295355665635 8 25.747298319437323 35.304828393655654 19.837189150966637 19.110684135940392 9 26.014292835035413 16.943008334589123 20.640279597064925 36.40241923331053 10-11 24.645887958110784 26.58498029558578 27.573599693784807 21.195532052518633 12-13 25.57137478337987 24.042880818297448 27.910879437495893 22.47486496082679 14-15 22.38979985741362 25.556796907150765 26.40624366041257 25.64715957502304 16-17 21.74685925989043 28.775329449124605 27.136293064447088 22.341518226537882 18-19 21.69925813325489 27.234849116085226 29.590839369438626 21.475053381221258 20-21 22.276580683835405 26.39879113094072 29.937379631959317 21.387248553264563 22-23 22.03637274257226 26.2111965604248 29.704029643554776 22.04840105344816 24-25 21.91770377318478 26.398276444132506 29.735194479124583 21.94882530355813 26-27 21.553671468957134 26.813699041875154 29.650103931032053 21.982525558135656 28-29 21.576976419626593 27.001911530485216 29.679126637807723 21.741985412080467 30-31 21.825345468042187 26.577339928321603 29.588302122888056 22.00901248074815 32-33 21.568591929576915 26.662975850759636 29.526305034340528 22.242127185322918 34-35 22.20837801459956 26.76245402582134 29.235005029858126 21.794162929720976 36-37 22.260107259081913 26.388922503465523 29.224846252562457 22.126123984890107 38-39 21.739686026051245 26.76341408818827 29.238767835889174 22.25813204987131 40-41 22.223402250015177 26.525429413804208 29.23792533341138 22.013243002769233 42-43 22.003918826623796 26.486055044967692 29.138376828039604 22.371649300368905 44-45 21.950649277281126 26.799257472175125 29.083204110411543 22.16688914013221 46-47 22.152915269376976 27.03258477864599 28.56607475039284 22.248425201584194 48-49 22.164224499283133 26.913409377117297 28.60267192895562 22.319694194643944 50-51 22.300116237430306 27.205802252665173 28.342478001226112 22.151603508678406 52-53 22.42058059770291 27.10602764356392 28.200627480566155 22.27276427816702 54-55 22.13857507325584 27.190504583089243 28.376910199959323 22.294010143695594 56-57 22.15056299276987 27.268669864122373 28.364901074096537 22.215866069011213 58-59 22.243054065756038 26.890224232109645 28.28819657251391 22.578525129620406 60-61 22.099142815876863 27.214042226288438 28.404605508571624 22.28220944926308 62-63 21.97728211309346 27.308527051383873 28.43520874124562 22.278982094277044 64-65 21.773348008240585 27.190132853492067 28.418572082080207 22.617947056187145 66-67 21.84352525670825 27.118770992818643 28.325662974378496 22.71204077609461 68-69 21.961844848851804 26.917625518656425 28.528873473544085 22.591656158947686 70-71 22.004763511599666 27.088339393609235 28.207340108034558 22.699556986756544 72-73 22.085480442472544 27.081420928432014 28.0431142992253 22.78998432987014 74-75 21.890117264190962 27.404362116642982 28.098307561790275 22.607213057375777 76-77 22.123331683896904 27.112809283816247 28.0622009430759 22.701658089210948 78-79 22.09219527372192 27.25667698165724 28.143773896024666 22.50735384859618 80-81 22.152611242215116 27.470377029110498 27.878313916872017 22.498697811802373 82-83 22.27856334247619 27.379133569980524 27.848990024459013 22.49331306308427 84-85 22.29150582226555 27.27242176987023 27.81043995440107 22.625632453463158 86-87 22.289782481584997 27.25660817500542 27.76084546866448 22.692763874745104 88-89 22.329927586836394 27.36136747579708 27.67149227911667 22.637212658249855 90-91 22.33868470521376 27.369316241285418 27.684666235148825 22.607332818352 92-93 22.40508194134958 26.90839730598464 27.837226744458103 22.84929400820768 94-95 22.302073520672828 26.91652897244391 27.87432433470252 22.907073172180738 96-97 22.415681140370705 26.8017367981204 27.83464137970204 22.947940681806855 98-99 22.43112415160604 26.864728237949727 27.545341241656583 23.158806368787648 100-101 22.77355485415548 26.303803590699644 27.764345049183998 23.158296505960877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1071.0 1 730.0 2 557.0 3 728.5 4 596.5 5 590.5 6 1171.5 7 2620.0 8 4462.5 9 5189.5 10 4321.0 11 3493.5 12 3601.5 13 3886.5 14 4145.5 15 4424.0 16 4641.0 17 4898.5 18 5243.0 19 5673.0 20 6421.0 21 7620.5 22 8990.0 23 11014.0 24 13793.5 25 17355.5 26 22109.0 27 27572.5 28 32386.5 29 37427.0 30 43917.0 31 51301.0 32 59328.0 33 65196.0 34 69482.0 35 73611.0 36 79717.0 37 87244.0 38 91627.0 39 94729.0 40 98551.5 41 105586.5 42 111837.5 43 118031.0 44 125232.0 45 130297.0 46 130744.5 47 130107.5 48 130968.0 49 127224.5 50 123458.0 51 119318.5 52 114958.0 53 112125.5 54 110295.5 55 103795.5 56 93797.5 57 85524.0 58 78482.0 59 70058.5 60 57102.0 61 46724.5 62 39011.0 63 29817.0 64 23293.5 65 19071.5 66 14128.5 67 11002.0 68 8461.5 69 5829.5 70 4297.0 71 3057.5 72 2215.0 73 1728.0 74 1297.5 75 1043.0 76 720.5 77 366.5 78 182.5 79 110.0 80 75.5 81 51.0 82 28.0 83 20.0 84 19.0 85 14.0 86 9.5 87 7.5 88 6.0 89 4.5 90 3.0 91 2.0 92 3.0 93 2.5 94 1.5 95 1.5 96 0.5 97 1.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4980802072495638 2 0.04883480050144013 3 0.05627877661583522 4 0.06519996199806746 5 0.08118857995583434 6 0.10850246896701941 7 0.07084811508097423 8 0.05288988476609115 9 0.05144164038585865 10-11 0.07514940089026478 12-13 0.10816937275956594 14-15 0.05549672465050967 16-17 0.060102141779649045 18-19 0.059044923382079305 20-21 0.07588800552418337 22-23 0.06553305820552094 24-25 0.0890815118281015 26-27 0.0675316354502418 28-29 0.06780680188248597 30-31 0.07132603572645096 32-33 0.07099293951899749 34-35 0.07407770004889273 36-37 0.06947228291975335 38-39 0.039783273124986966 40-41 0.04349077873838218 42-43 0.04121703506141715 44-45 0.021245745058010877 46-47 0.02845800207156876 48-49 0.028371107408754814 50-51 0.026068398844185128 52-53 0.02534427665406887 54-55 0.02473601401437122 56-57 0.024837391120987495 58-59 0.02150642904645273 60-61 0.02840007229635946 62-63 0.019768535790173722 64-65 0.021984349691929455 66-67 0.015394837761871548 68-69 0.018204431859522613 70-71 0.04143427171845202 72-73 0.03777021343646378 74-75 0.03197723591553375 76-77 0.022360893230789908 78-79 0.015119671329627372 80-81 0.01662584548506918 82-83 0.022114691686150382 84-85 0.017987195202487736 86-87 0.009022562488848519 88-89 0.041303929724231095 90-91 0.053454700074381835 92-93 0.03407719026687089 94-95 0.05432364670252133 96-97 0.04367905050781241 98-99 0.03346892762717324 100-101 0.1696618291442382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3452456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.639664015754455 #Duplication Level Percentage of deduplicated Percentage of total 1 69.68171206525138 32.49931638765867 2 15.282769824604593 14.255664996993374 3 5.959606655029367 8.338621561698718 4 2.996263072130757 5.58978811947963 5 1.718341316874785 4.007143084171451 6 1.0137446120692142 2.8368424862813697 7 0.6837055772972419 2.232145888758856 8 0.507613831426822 1.8939950837997437 9 0.35724510518077407 1.4995612509213747 >10 1.6176134868540335 13.167556310411346 >50 0.10667759924311573 3.3930033776165387 >100 0.0673462754397769 6.0121652347337005 >500 0.004615956073830121 1.4813175490717836 >1k 0.0025574891704618265 1.77937317177152 >5k 6.23777846454104E-5 0.22258893000921975 >10k+ 1.247555692908208E-4 0.7909165666227483 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15402 0.44611719888682144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11784 0.34132223553319724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7651 0.2216103550631782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8964887604650138E-5 2 0.0 0.0 0.0 0.0 2.8964887604650138E-5 3 0.0 0.0 0.0 0.0 2.8964887604650138E-5 4 0.0 0.0 0.0 0.0 2.8964887604650138E-5 5 0.0 0.0 0.0 0.0 2.8964887604650138E-5 6 0.0 0.0 0.0 0.0 2.8964887604650138E-5 7 0.0 0.0 0.0 0.0 2.8964887604650138E-5 8 0.0 0.0 0.0 2.8964887604650138E-5 2.8964887604650138E-5 9 0.0 0.0 0.0 5.7929775209300276E-5 2.8964887604650138E-5 10-11 0.0 0.0 0.0 5.7929775209300276E-5 2.8964887604650138E-5 12-13 0.0 0.0 0.0 5.7929775209300276E-5 5.7929775209300276E-5 14-15 0.0 0.0 0.0 8.689466281395041E-5 7.241221901162535E-5 16-17 0.0 0.0 0.0 1.3034199422092563E-4 8.689466281395041E-5 18-19 0.0 0.0 0.0 1.5930688182557576E-4 8.689466281395041E-5 20-21 0.0 0.0 0.0 2.896488760465014E-4 8.689466281395041E-5 22-23 0.0 0.0 0.0 4.0550842646510196E-4 8.689466281395041E-5 24-25 0.0 0.0 0.0 6.22745083499978E-4 8.689466281395041E-5 26-27 0.0 0.0 0.0 0.001274455054604606 8.689466281395041E-5 28-29 0.0 0.0 0.0 0.004257838477883571 8.689466281395041E-5 30-31 0.0 0.0 0.0 0.012469384113801885 8.689466281395041E-5 32-33 0.0 0.0 0.0 0.03284618254367326 8.689466281395041E-5 34-35 0.0 0.0 0.0 0.060724886863149016 1.1585955041860055E-4 36-37 0.0 1.4482443802325069E-5 0.0 0.10690940014876366 1.1585955041860055E-4 38-39 0.0 2.8964887604650138E-5 0.0 0.17261624767991252 1.1585955041860055E-4 40-41 0.0 2.8964887604650138E-5 0.0 0.2434353978732821 1.1585955041860055E-4 42-43 0.0 2.8964887604650138E-5 0.0 0.3193958156164771 1.1585955041860055E-4 44-45 0.0 2.8964887604650138E-5 0.0 0.4103310802512762 1.1585955041860055E-4 46-47 0.0 2.8964887604650138E-5 0.0 0.5154446573685516 1.1585955041860055E-4 48-49 0.0 2.8964887604650138E-5 0.0 0.620384445160199 1.1585955041860055E-4 50-51 0.0 2.8964887604650138E-5 0.0 0.7336516381381835 1.1585955041860055E-4 52-53 0.0 2.8964887604650138E-5 0.0 0.8457167882805747 1.448244380232507E-4 54-55 0.0 2.8964887604650138E-5 0.0 0.9658631420646635 1.7378932562790082E-4 56-57 0.0 2.8964887604650138E-5 0.0 1.1020994909131354 1.7378932562790082E-4 58-59 0.0 2.8964887604650138E-5 0.0 1.2451425883487002 1.7378932562790082E-4 60-61 0.0 2.8964887604650138E-5 0.0 1.3973965200425438 1.7378932562790082E-4 62-63 0.0 2.8964887604650138E-5 0.0 1.5469567171891545 2.0275421323255098E-4 64-65 0.0 2.8964887604650138E-5 0.0 1.7111586650199162 2.0275421323255098E-4 66-67 0.0 2.8964887604650138E-5 0.0 1.8928698874076888 2.0275421323255098E-4 68-69 0.0 2.8964887604650138E-5 0.0 2.088672527615124 2.0275421323255098E-4 70-71 0.0 2.8964887604650138E-5 0.0 2.292817055452698 2.0275421323255098E-4 72-73 0.0 2.8964887604650138E-5 0.0 2.4977146703679933 2.0275421323255098E-4 74-75 1.4482443802325069E-5 2.8964887604650138E-5 0.0 2.713372741028416 2.1723665703487604E-4 76-77 2.8964887604650138E-5 2.8964887604650138E-5 0.0 2.9415581255778496 2.6068398844185126E-4 78-79 2.8964887604650138E-5 2.8964887604650138E-5 0.0 3.187513468672736 2.6068398844185126E-4 80-81 2.8964887604650138E-5 2.8964887604650138E-5 0.0 3.4576110455860984 2.6068398844185126E-4 82-83 2.8964887604650138E-5 2.8964887604650138E-5 0.0 3.730228567721066 2.751664322441763E-4 84-85 2.8964887604650138E-5 5.7929775209300276E-5 0.0 4.010043864425788 2.896488760465014E-4 86-87 2.8964887604650138E-5 5.7929775209300276E-5 0.0 4.297723128115173 2.896488760465014E-4 88-89 2.8964887604650138E-5 5.7929775209300276E-5 0.0 4.6050261031567095 2.896488760465014E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7400 0.0 47.758095 1 GTATCAA 12715 0.0 41.917446 1 ATCAACG 15715 0.0 33.47794 3 TATCAAC 15870 0.0 33.300697 2 TCAACGC 16030 0.0 32.849724 4 CAACGCA 16155 0.0 32.47787 5 AACGCAG 16630 0.0 31.607368 6 ACGCAGA 18610 0.0 28.050797 7 CGCAGAG 18820 0.0 27.762255 8 GCAGAGT 20385 0.0 25.37324 9 GAGTACT 13270 0.0 23.407766 12-13 GTACATG 10465 0.0 23.320004 1 TACATGG 10365 0.0 22.925888 2 GTACTTT 13835 0.0 22.027412 14-15 ACATGGG 10355 0.0 21.800627 3 CAGAGTA 19845 0.0 21.367601 10-11 AGTACTT 13810 0.0 20.978062 12-13 AGAGTAC 18570 0.0 20.940325 10-11 GGACCGA 1420 0.0 20.750511 6 AGGACCG 1610 0.0 20.663202 5 >>END_MODULE