FastQCFastQC Report
Thu 26 May 2016
SRR938189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452456
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148130.4290568800876825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116870.33851264143554616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79300.2296915587048756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.045.889111
GTATCAA121900.039.2103961
ATCAACG151900.031.1772173
TATCAAC153750.030.9950772
TCAACGC154500.030.6498874
CAACGCA156650.030.1382625
AACGCAG162100.029.1831576
ACGCAGA182450.025.7459167
CGCAGAG184450.025.28658
GGACCGA15650.023.0650926
GAGTACT128050.022.81137712-13
GCAGAGT202050.022.6372369
GTACATG102900.022.1632181
AGGACCG17650.021.797295
TACATGG102450.021.3794172
GTACTTT134450.021.3016114-15
ACATGGG99250.021.1061043
AGAGTAC182400.020.51905310-11
ACCGAGT17800.020.279148
AGTACTT135700.020.16037412-13
Produced by FastQC (version 0.11.5)