##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938189_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3452456 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54622651237264 34.0 31.0 34.0 31.0 34.0 2 32.76315469335453 34.0 31.0 34.0 31.0 34.0 3 32.82346625127156 34.0 31.0 34.0 31.0 34.0 4 36.269110453543796 37.0 37.0 37.0 35.0 37.0 5 36.23231838436174 37.0 37.0 37.0 35.0 37.0 6 36.26846540549684 37.0 37.0 37.0 35.0 37.0 7 36.235695110958694 37.0 37.0 37.0 35.0 37.0 8 36.230066074701604 37.0 37.0 37.0 35.0 37.0 9 37.95047409728031 39.0 38.0 39.0 35.0 39.0 10-11 37.936490139193666 39.0 38.0 39.0 35.0 39.0 12-13 37.887703710054524 39.0 38.0 39.0 35.0 39.0 14-15 39.2356738217663 41.0 39.0 41.0 36.0 41.0 16-17 39.194431442428225 40.0 39.0 41.0 36.0 41.0 18-19 39.22561257840795 40.0 39.0 41.0 36.0 41.0 20-21 39.15191185637123 40.0 39.0 41.0 36.0 41.0 22-23 39.01329386963947 40.0 38.5 41.0 35.0 41.0 24-25 38.98458937637439 40.0 38.5 41.0 35.0 41.0 26-27 38.95411020444576 40.0 38.0 41.0 35.0 41.0 28-29 38.90026621628198 40.0 38.5 41.0 35.0 41.0 30-31 38.71707358471766 40.0 38.0 41.0 35.0 41.0 32-33 38.552010221129535 40.0 38.0 41.0 34.5 41.0 34-35 38.3612942496588 40.0 38.0 41.0 34.0 41.0 36-37 38.23598099439935 40.0 38.0 41.0 34.0 41.0 38-39 38.221449310288094 40.0 38.0 41.0 34.0 41.0 40-41 38.16817158567698 40.0 38.0 41.0 34.0 41.0 42-43 38.02296061122864 40.0 38.0 41.0 33.5 41.0 44-45 37.82474403728824 40.0 38.0 41.0 33.0 41.0 46-47 37.52003023354968 40.0 37.0 41.0 33.0 41.0 48-49 37.20026221912748 40.0 37.0 41.0 32.0 41.0 50-51 36.92628320245066 40.0 36.5 41.0 31.0 41.0 52-53 36.53494454382619 39.5 36.0 41.0 30.5 41.0 54-55 36.32971122586356 39.0 35.5 41.0 30.0 41.0 56-57 36.10304403589792 39.0 35.0 41.0 30.0 41.0 58-59 35.79382126810595 39.0 35.0 40.0 29.0 41.0 60-61 35.46313827026326 38.0 35.0 40.0 28.0 41.0 62-63 35.12092579890953 38.0 34.5 40.0 28.0 41.0 64-65 34.75700399947168 37.0 34.0 40.0 27.5 41.0 66-67 34.356639737045164 37.0 34.0 39.5 26.0 41.0 68-69 33.916482353431874 36.0 34.0 39.0 26.0 41.0 70-71 33.41827629374567 35.5 33.0 39.0 25.5 40.0 72-73 33.009057320353975 35.0 33.0 38.0 25.0 40.0 74-75 32.56366916768816 35.0 32.0 37.0 24.0 39.5 76-77 31.222604285181333 34.0 30.5 36.0 22.5 38.5 78-79 31.930966679951894 35.0 32.0 36.0 24.0 39.0 80-81 31.769052234119712 35.0 32.0 36.0 23.0 38.0 82-83 31.398284728320938 35.0 32.0 35.5 21.5 37.0 84-85 31.018062214261384 34.5 31.5 35.0 20.0 37.0 86-87 30.59536240288073 34.0 31.0 35.0 18.0 36.0 88-89 30.15040582704023 34.0 31.0 35.0 16.0 36.0 90-91 29.821669414469007 34.0 30.0 35.0 9.0 35.5 92-93 29.438645995778078 34.0 30.0 35.0 4.5 35.0 94-95 29.049816275717923 34.0 29.5 35.0 2.0 35.0 96-97 28.509048486063257 34.0 29.0 35.0 2.0 35.0 98-99 27.864398271838947 34.0 29.0 35.0 2.0 35.0 100-101 25.43954694860702 31.5 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06712268219820317 1101 2 0.1105066087633233 1101 3 0.10473147578800734 1101 4 0.05399204307606453 1101 5 0.0839599166022964 1101 6 0.06591420794550373 1101 7 0.13968515225148082 1101 8 0.08695602041404271 1101 9 0.08153321208040865 1101 10-11 0.13051867263231287 1101 12-13 0.11749273408781846 1101 14-15 0.09408293776076704 1101 16-17 0.1438447866195176 1101 18-19 0.15310699288286145 1101 20-21 0.1777299613479002 1101 22-23 0.16757985983811352 1101 24-25 0.17764292447677832 1101 26-27 0.20472939981941352 1101 28-29 0.2326458008610004 1101 30-31 0.18163645339067358 1101 32-33 0.25742592704601464 1101 34-35 0.1253088235056481 1101 36-37 0.19242566723277577 1101 38-39 0.277384136671877 1101 40-41 0.3567805243930593 1101 42-43 0.33537926861046685 1101 44-45 0.3742592868458985 1101 46-47 0.4316090131809105 1101 48-49 0.45482132593048163 1101 50-51 0.4072285665236208 1101 52-53 0.31809657268726 1101 54-55 0.39688505660066653 1101 56-57 0.4627611564881349 1101 58-59 0.4828682172525518 1101 60-61 0.39250324143458215 1101 62-63 0.36563221761820586 1101 64-65 0.4750280321470015 1101 66-67 0.43881716386991343 1101 68-69 0.4112187630621946 1101 70-71 0.5120336013162046 1101 72-73 0.46353973673736704 1101 74-75 0.4493024730919757 1101 76-77 0.3756724543169341 1101 78-79 0.45504216129128494 1101 80-81 0.5267977291001458 1101 82-83 0.5682195706874289 1101 84-85 0.6080415870117051 1101 86-87 0.6830943856158314 1101 88-89 0.6161549677933742 1101 90-91 0.6389919337282493 1101 92-93 0.6891816522880028 1101 94-95 0.6370001381447175 1101 96-97 0.6551280278360174 1101 98-99 0.7156271807201442 1101 100-101 1.00402759736399 1102 1 0.16022071815368122 1102 2 0.10242333458056407 1102 3 0.13122973915529457 1102 4 0.05485591327559547 1102 5 0.06648119896915006 1102 6 0.058947261583014665 1102 7 0.07239914533862901 1102 8 0.11390237238037315 1102 9 0.07678355711810525 1102 10-11 0.13931612090559753 1102 12-13 0.12765986229556603 1102 14-15 0.15964709740784855 1102 16-17 0.11335045270931943 1102 18-19 0.15933666617701903 1102 20-21 0.14152397108313863 1102 22-23 0.22506932191606666 1102 24-25 0.15031772822755585 1102 26-27 0.0780496595171627 1102 28-29 0.00971098891246669 1102 30-31 0.05000563924905066 1102 32-33 -0.02667897704061062 1102 34-35 0.059445587344121975 1102 36-37 0.04431649483390032 1102 38-39 -0.035305252583782476 1102 40-41 0.054061264318036706 1102 42-43 0.048683490566915566 1102 44-45 0.05207790146497615 1102 46-47 -0.00964244629037836 1102 48-49 -0.1711489232175083 1102 50-51 -0.07626229412114327 1102 52-53 -0.047311734631215074 1102 54-55 -0.07455334673905867 1102 56-57 -0.06639754085580307 1102 58-59 -0.1443967848494978 1102 60-61 -0.12131073655140767 1102 62-63 -0.18887337217656608 1102 64-65 -0.11117824751269723 1102 66-67 -0.24253922494428082 1102 68-69 -0.05682614261346686 1102 70-71 -0.04843773358898318 1102 72-73 -0.022578619478331063 1102 74-75 0.0028280663910678072 1102 76-77 -0.06255235503757461 1102 78-79 0.020743020583775973 1102 80-81 0.11958164000439098 1102 82-83 0.14636867432606948 1102 84-85 0.02586384274527731 1102 86-87 -0.14031219269943662 1102 88-89 -0.181525031911967 1102 90-91 -0.2247620342709844 1102 92-93 -0.2130123296653288 1102 94-95 -0.27143125184021955 1102 96-97 -0.10248245394341637 1102 98-99 -0.06727773410039717 1102 100-101 0.07186503317750592 1103 1 0.13047272764939066 1103 2 0.1563407590297956 1103 3 0.1212217273355165 1103 4 0.07987095703454372 1103 5 0.08671245079253964 1103 6 0.04815692493355783 1103 7 0.09780436422717287 1103 8 0.07338635787320413 1103 9 0.05181195391001836 1103 10-11 0.08057329044765282 1103 12-13 0.13835393863345047 1103 14-15 0.17576852756950956 1103 16-17 0.11159481271894123 1103 18-19 0.17746628389523522 1103 20-21 0.13722187561956645 1103 22-23 0.11746362192342019 1103 24-25 0.19493092714528615 1103 26-27 0.18966354015536524 1103 28-29 0.21138813972571313 1103 30-31 0.18219281668444864 1103 32-33 0.20819070377500992 1103 34-35 0.14508098191513596 1103 36-37 0.121141652046731 1103 38-39 0.23108681714620616 1103 40-41 0.2839610992933075 1103 42-43 0.18256855180574405 1103 44-45 0.17047907199036416 1103 46-47 0.18466264558391998 1103 48-49 0.2466986764699186 1103 50-51 0.1849656320318971 1103 52-53 0.17873966245145567 1103 54-55 0.27593345893408383 1103 56-57 0.3024984817174854 1103 58-59 0.2527247850130223 1103 60-61 0.24176163589997657 1103 62-63 0.4266722732290802 1103 64-65 0.3366896359501297 1103 66-67 0.3258812716898163 1103 68-69 0.3591653114454303 1103 70-71 0.40318780688798483 1103 72-73 0.20532452981350957 1103 74-75 0.20126578621629676 1103 76-77 0.31883198671713586 1103 78-79 0.25052734318515846 1103 80-81 0.36826245413955405 1103 82-83 0.4232845136888379 1103 84-85 0.4682821091020024 1103 86-87 0.5036726978871471 1103 88-89 0.3020281780732361 1103 90-91 0.35831594596060157 1103 92-93 0.43456460083521975 1103 94-95 0.30511020895690777 1103 96-97 0.3308632040007531 1103 98-99 0.36765236857232964 1103 100-101 0.5119867753213079 1104 1 0.14464099267239305 1104 2 0.127596880889719 1104 3 0.07212394517130605 1104 4 0.03294492213761657 1104 5 0.015530349900565454 1104 6 0.04742509498625225 1104 7 0.07376943005462522 1104 8 0.02102094568348889 1104 9 0.06452680093335061 1104 10-11 0.08454970615685653 1104 12-13 0.11826609110732988 1104 14-15 0.14284735291727202 1104 16-17 0.09283398889814976 1104 18-19 0.054528163562281406 1104 20-21 0.06763215661992916 1104 22-23 0.102401518796853 1104 24-25 0.15049925920614982 1104 26-27 0.1745029751752014 1104 28-29 0.2112428910338835 1104 30-31 0.12662960557106118 1104 32-33 0.09384739545672716 1104 34-35 0.21202107614057297 1104 36-37 0.22380431195995243 1104 38-39 0.26503101744042823 1104 40-41 0.25293234750098037 1104 42-43 0.27610568333030017 1104 44-45 0.24862635975036795 1104 46-47 0.2824322401910919 1104 48-49 0.19527482030336785 1104 50-51 0.28784849268625834 1104 52-53 0.27484192311698763 1104 54-55 0.30217971133161825 1104 56-57 0.3214762474946653 1104 58-59 0.36466567065760813 1104 60-61 0.31612524179185897 1104 62-63 0.3293707797745782 1104 64-65 0.41857917245783227 1104 66-67 0.39439838496517154 1104 68-69 0.38389273737791285 1104 70-71 0.3697058884879496 1104 72-73 0.3877822587198594 1104 74-75 0.39221870143202864 1104 76-77 0.31413933667337446 1104 78-79 0.34704509890665136 1104 80-81 0.42321254817876763 1104 82-83 0.5074191201207476 1104 84-85 0.6221007065210422 1104 86-87 0.5226353335922518 1104 88-89 0.5064794452749624 1104 90-91 0.4634855424299431 1104 92-93 0.6034315679308087 1104 94-95 0.386662461242711 1104 96-97 0.559933658927104 1104 98-99 0.6658092930243882 1104 100-101 0.7096648911850743 1105 1 0.1478429423274008 1105 2 0.11358443882725311 1105 3 0.09020326771542386 1105 4 0.060257148275091765 1105 5 0.031065344520023075 1105 6 0.02834281138600403 1105 7 0.014922483599804082 1105 8 0.03826065759594144 1105 9 -0.0071532643364875526 1105 10-11 0.026782296764466196 1105 12-13 0.09083631948142568 1105 14-15 0.11310421222842848 1105 16-17 0.08943000518747368 1105 18-19 0.06472798210088371 1105 20-21 0.09967600627422968 1105 22-23 0.06106383883005151 1105 24-25 0.0908067237402932 1105 26-27 0.06438412851007769 1105 28-29 -3.168329759901667E-4 1105 30-31 0.08136096533586823 1105 32-33 0.09367753914883536 1105 34-35 0.16020083545804908 1105 36-37 0.17444320729611462 1105 38-39 0.08917443876143238 1105 40-41 0.09389567541860089 1105 42-43 0.00912418465609477 1105 44-45 0.18219487197791295 1105 46-47 0.17532799132171561 1105 48-49 0.15653729577442732 1105 50-51 0.1987411878100147 1105 52-53 0.1702230096346895 1105 54-55 0.32226179096362984 1105 56-57 0.23277450532457777 1105 58-59 0.23414525273372533 1105 60-61 0.36177966333090694 1105 62-63 0.30088564835573095 1105 64-65 0.25813564704268543 1105 66-67 0.25590280896589945 1105 68-69 0.25639808275240483 1105 70-71 0.3372541023592035 1105 72-73 0.2589665580889644 1105 74-75 0.33659102434891963 1105 76-77 0.4009524384675949 1105 78-79 0.2524017268193539 1105 80-81 0.23725031043620248 1105 82-83 0.36580687428978464 1105 84-85 0.5012031935268588 1105 86-87 0.5402874657636687 1105 88-89 0.28984328592653696 1105 90-91 0.28660015499579217 1105 92-93 0.37189649594408536 1105 94-95 0.2969525708932217 1105 96-97 0.48731613102214055 1105 98-99 0.40794895499978523 1105 100-101 0.3373761741452874 1106 1 -0.13211623728130917 1106 2 -0.09403640700049465 1106 3 -0.030787413070385128 1106 4 -0.07678757476556086 1106 5 -0.029688434484533843 1106 6 -0.040186926909484555 1106 7 0.026126128531473114 1106 8 -0.07251603970781417 1106 9 -0.0820743076941497 1106 10-11 -0.03319222653658471 1106 12-13 -0.12262466894608792 1106 14-15 -0.2537063518501057 1106 16-17 -0.2178832626468008 1106 18-19 -0.13817441008670528 1106 20-21 -0.07073789452088164 1106 22-23 -0.07834178757048704 1106 24-25 -0.030352868431343438 1106 26-27 -0.09958121338836179 1106 28-29 0.021162408275735345 1106 30-31 -0.05186149841986065 1106 32-33 -0.04110343601390554 1106 34-35 0.015840027996240735 1106 36-37 -0.04640746565019782 1106 38-39 -0.057131238706503495 1106 40-41 0.004039763955994147 1106 42-43 -0.14239955531526505 1106 44-45 0.02313441494645474 1106 46-47 0.020551344984767184 1106 48-49 -0.024810662350972734 1106 50-51 0.0723395586156883 1106 52-53 -0.088866183289106 1106 54-55 -0.3643375928073951 1106 56-57 -0.1912997848368505 1106 58-59 -0.13148812891748207 1106 60-61 -0.1773648587596881 1106 62-63 -0.13533609482318099 1106 64-65 -0.2730995470151285 1106 66-67 -0.2136876928325222 1106 68-69 -0.28892071632201777 1106 70-71 -0.31792248254932787 1106 72-73 -0.12208319390050093 1106 74-75 -0.341387088759177 1106 76-77 -0.2347229706835634 1106 78-79 -0.15937933518814162 1106 80-81 -0.39181534051558486 1106 82-83 -0.4105593128529641 1106 84-85 -0.16527905376075225 1106 86-87 -0.19256807048932245 1106 88-89 -0.14902018103037307 1106 90-91 -0.2686503820837345 1106 92-93 -0.19006908603485684 1106 94-95 -0.3573906899029389 1106 96-97 -0.15785377455178917 1106 98-99 0.041261532770274556 1106 100-101 0.21100681834229462 1107 1 0.04401188343766904 1107 2 0.036278421456422905 1107 3 -0.003095492134704614 1107 4 0.00484109408301947 1107 5 0.0019586114231131546 1107 6 0.06798378403608041 1107 7 0.03729110678926162 1107 8 -0.007630224092565641 1107 9 0.02331263679758422 1107 10-11 0.05887095236050044 1107 12-13 0.0463077591646055 1107 14-15 0.06694707590896343 1107 16-17 0.033870433846921344 1107 18-19 0.05142600423244659 1107 20-21 0.04703383535703409 1107 22-23 0.06320317417181798 1107 24-25 0.001978583706360837 1107 26-27 -0.028268359470871474 1107 28-29 -0.041563904013337094 1107 30-31 0.011999895595430132 1107 32-33 0.03308912692553889 1107 34-35 0.07148775170710309 1107 36-37 0.11862417312205054 1107 38-39 0.05781169694514432 1107 40-41 0.1359138580306265 1107 42-43 0.11876287720274092 1107 44-45 0.06268494867671137 1107 46-47 -0.06254072646778042 1107 48-49 -0.05537139336014718 1107 50-51 -0.05205029915700976 1107 52-53 -0.11671474677587668 1107 54-55 -0.16798348994996815 1107 56-57 -0.15792251097411736 1107 58-59 -0.061169943266229154 1107 60-61 -0.07664250509596826 1107 62-63 -0.05027202969042577 1107 64-65 -0.025026274259786874 1107 66-67 -0.20207601679700105 1107 68-69 -0.07086354537081263 1107 70-71 -0.12536845320013157 1107 72-73 -0.2029942823474542 1107 74-75 -0.0654887210557007 1107 76-77 -0.13342432517623948 1107 78-79 -0.1120778193592109 1107 80-81 -0.044283196573093875 1107 82-83 -0.0565343391456814 1107 84-85 -0.038939159313486016 1107 86-87 0.05188348965139156 1107 88-89 -0.026057767287195333 1107 90-91 -0.06930781938295993 1107 92-93 0.1249405957317613 1107 94-95 0.13061959170360993 1107 96-97 0.2995212336025368 1107 98-99 0.40232850004638365 1107 100-101 0.6459667468070087 1108 1 0.02497899734034803 1108 2 0.01021152787125601 1108 3 0.041238418202205196 1108 4 0.020358917902001394 1108 5 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scores pass #Quality Count 2 15.0 3 10.0 4 30.0 5 40.0 6 93.0 7 243.0 8 605.0 9 1143.0 10 2138.0 11 3596.0 12 5605.0 13 8273.0 14 11224.0 15 15110.0 16 19398.0 17 27593.0 18 27430.0 19 18828.0 20 15617.0 21 15972.0 22 15785.0 23 17261.0 24 20435.0 25 23745.0 26 28473.0 27 33980.0 28 42283.0 29 51275.0 30 63277.0 31 78583.0 32 99085.0 33 129492.0 34 181210.0 35 275616.0 36 466832.0 37 821970.0 38 826270.0 39 103915.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12456291984605 19.3716299353272 15.269101184779762 23.23470596004699 2 15.326299066243628 20.12663303073354 40.44388770820635 24.10318019481648 3 18.72327273390875 24.1424898029768 30.28969944960262 26.84453801351183 4 12.82240816392736 17.21614989445195 37.485807205073726 32.475634736546965 5 13.498133936575568 37.25294029900462 34.760076525255215 14.488849239164594 6 31.685298813366487 36.86566896145816 17.31132851512083 14.137703710054522 7 28.239577854142095 32.85718920096303 20.80182919058201 18.101403754312873 8 25.80726823822878 35.461959433978116 19.71106861627959 19.019703711513518 9 25.8908042481235 16.947366530704933 20.635567520033646 36.52626170113792 10-11 24.65672263455349 26.73683603788144 27.468184967455052 21.13825636011002 12-13 25.462453395495842 24.051964746256 27.95569299072892 22.529888867519237 14-15 22.28467212905827 25.595561536483014 26.403247427338684 25.716518907120033 16-17 21.783291935153812 28.84600818578941 27.14745905548428 22.223240823572503 18-19 21.638769617918374 27.308342235208787 29.624244306082392 21.42864384079044 20-21 22.176068282984634 26.433689524211168 29.980917932046058 21.40932426075814 22-23 22.0213668182882 26.28470862481665 29.74911193654604 21.944812620349108 24-25 21.967460184812108 26.459513658142498 29.73138199494071 21.841644162104686 26-27 21.554205280808105 26.906253118911106 29.647780508618084 21.891761091662705 28-29 21.61209921635474 27.048723299796812 29.679445496031086 21.659731987817366 30-31 21.83251575110588 26.607000929193592 29.56616970643507 21.994313613265454 32-33 21.556014328985928 26.66151572401278 29.541665067557243 22.240804879444045 34-35 22.20027058503149 26.812065079466247 29.26104792310055 21.726616412401718 36-37 22.21693593424495 26.477133690657435 29.298389376675683 22.007540998421934 38-39 21.724774426936285 26.75189128516135 29.345697247495124 22.17763704040724 40-41 22.211606511994674 26.532565912117562 29.275223811070227 21.980603764817534 42-43 22.025224090544633 26.50426861202905 29.1758734201269 22.294633877299418 44-45 21.954134439432593 26.829694652011042 29.146347910024197 22.069822998532167 46-47 22.17043782316673 27.05665576283315 28.57521262765009 22.197693786350033 48-49 22.131360978966857 26.91998102220564 28.664116211763453 22.28454178706405 50-51 22.28929202863121 27.18980343268676 28.368326779544766 22.152577759137262 52-53 22.409467347302904 27.115711250194064 28.27319160620729 22.201629796295737 54-55 22.12675556183772 27.19074479153391 28.382519574471043 22.29998007215733 56-57 22.173287653774587 27.301158943082836 28.334524755710138 22.191028647432436 58-59 22.27653299564136 26.97618738660246 28.242242623801722 22.505036993954448 60-61 22.095719684769335 27.30233202103082 28.365705457216546 22.236242836983294 62-63 21.966116295182328 27.41612058198569 28.353424344872174 22.264338777959804 64-65 21.76605581649701 27.26288184411329 28.30260544956981 22.668456889819886 66-67 21.751327171150045 27.266763139052312 28.262373220686953 22.719536469110686 68-69 21.918112549769063 27.04698350097293 28.43611456920688 22.59878938005113 70-71 21.966391461614574 27.22921016227289 28.078113088189973 22.72628528792257 72-73 22.036472007174023 27.27239680969142 27.86979761653733 22.82133356659723 74-75 21.848301614850413 27.5818431864157 27.89926938967506 22.670585809058828 76-77 21.988766837289162 27.281752468387722 27.962514221759815 22.7669664725633 78-79 21.93367562106512 27.463594032769716 28.04073390073617 22.561996445428996 80-81 22.008940881505804 27.677195596410208 27.746580984667148 22.56728253741684 82-83 22.19277939691593 27.585001856650372 27.71531496147378 22.506903784959917 84-85 22.059244810713157 27.462834768055295 27.743643248532234 22.734277172699315 86-87 22.145077104888138 27.45959379453372 27.56824159145416 22.82708750912398 88-89 22.111647026519123 27.655561902091613 27.48484265675526 22.747948414634006 90-91 22.05225594175961 27.653916443962466 27.551858470341127 22.741969143936792 92-93 22.048485596307316 27.2373834450222 27.711814920890916 23.00231603777957 94-95 21.866071573395867 27.322636407241685 27.70255435550808 23.10873766385437 96-97 21.965522514986432 27.19765291722762 27.730331682720937 23.10649288506501 98-99 21.887969028424983 27.290340557562498 27.485013567153356 23.336676846859163 100-101 21.863175084635404 27.188644837182572 27.642350836621816 23.30582924156021 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1071.0 1 733.0 2 546.5 3 711.0 4 579.5 5 559.0 6 1161.5 7 2658.5 8 4423.0 9 5110.5 10 4318.0 11 3478.5 12 3507.5 13 3864.0 14 4175.5 15 4343.5 16 4487.0 17 4699.0 18 5081.0 19 5533.5 20 6266.0 21 7404.0 22 8745.0 23 10568.5 24 13287.5 25 16895.5 26 21605.0 27 27198.0 28 32186.5 29 37522.5 30 44108.5 31 51154.0 32 59477.0 33 65590.0 34 69631.5 35 74100.0 36 79987.0 37 87146.5 38 91953.0 39 95283.5 40 99069.0 41 106277.0 42 112373.5 43 118783.5 44 126819.5 45 131904.0 46 131728.5 47 132027.0 48 133009.5 49 128703.0 50 125102.5 51 120407.5 52 115204.5 53 112734.0 54 111905.5 55 104709.0 56 94226.0 57 85730.0 58 78193.5 59 69354.0 60 55806.5 61 45448.0 62 37815.0 63 28377.5 64 21854.5 65 17357.0 66 12416.5 67 9833.5 68 7440.0 69 4853.5 70 3518.5 71 2488.0 72 1814.5 73 1435.0 74 1132.5 75 907.5 76 568.0 77 253.0 78 120.0 79 60.5 80 28.5 81 20.5 82 11.5 83 7.0 84 5.0 85 2.0 86 1.5 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01714721346195288 3 0.006459169935836981 4 0.0 5 2.8964887604650138E-5 6 0.0 7 0.0 8 2.896488760465014E-4 9 4.0550842646510196E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.751664322441763E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005590223307697477 26-27 0.021158850395196927 28-29 2.8964887604650138E-5 30-31 0.0 32-33 3.9102598266277686E-4 34-35 0.019580264020743494 36-37 0.0037075056133952177 38-39 0.018436150960359814 40-41 0.015901723294952926 42-43 0.008110168529302039 44-45 0.0024185681149882865 46-47 1.4482443802325069E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.896488760465014E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.7929775209300276E-5 84-85 0.0029833834232789644 86-87 4.634382016744022E-4 88-89 1.1585955041860055E-4 90-91 1.8827176943022591E-4 92-93 2.317191008372011E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3452456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.52689251849366 #Duplication Level Percentage of deduplicated Percentage of total 1 67.54504188652946 30.07570820238649 2 16.145352073357287 14.378047128872373 3 6.446542498565631 8.611335148486003 4 3.210155028862174 5.717529117513917 5 1.811771798459484 4.033628406902171 6 1.18600963065845 3.168559401013629 7 0.7665270871806615 2.389174845638513 8 0.5397294447400237 1.9225979980004237 9 0.3828425395155405 1.534210974766407 >10 1.7661625778404078 13.719301652733007 >50 0.11843911562574405 3.6584780036860587 >100 0.07309224783224579 6.201477276817558 >500 0.005208794273311628 1.6042116678466916 >1k 0.0029299467746953366 1.9875653160162088 >5k 6.510992832656304E-5 0.22990202249235725 >10k+ 1.302198566531261E-4 0.76827283682818 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14813 0.4290568800876825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11687 0.33851264143554616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7930 0.2296915587048756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.8964887604650138E-5 6 0.0 0.0 0.0 0.0 2.8964887604650138E-5 7 0.0 0.0 0.0 0.0 2.8964887604650138E-5 8 0.0 0.0 0.0 0.0 2.8964887604650138E-5 9 0.0 0.0 0.0 2.8964887604650138E-5 2.8964887604650138E-5 10-11 0.0 0.0 0.0 2.8964887604650138E-5 2.8964887604650138E-5 12-13 0.0 0.0 0.0 2.8964887604650138E-5 5.7929775209300276E-5 14-15 0.0 0.0 0.0 2.8964887604650138E-5 7.241221901162535E-5 16-17 0.0 0.0 0.0 7.241221901162535E-5 1.0137710661627548E-4 18-19 0.0 0.0 0.0 1.0137710661627548E-4 1.1585955041860055E-4 20-21 0.0 0.0 0.0 2.317191008372011E-4 1.1585955041860055E-4 22-23 1.4482443802325069E-5 0.0 0.0 3.4757865125580165E-4 1.448244380232507E-4 24-25 2.8964887604650138E-5 0.0 0.0 5.358504206860275E-4 1.448244380232507E-4 26-27 2.8964887604650138E-5 0.0 0.0 0.0011875603917906557 1.448244380232507E-4 28-29 2.8964887604650138E-5 0.0 0.0 0.00419990870267427 1.448244380232507E-4 30-31 2.8964887604650138E-5 0.0 0.0 0.01245490166999956 1.448244380232507E-4 32-33 2.8964887604650138E-5 0.0 0.0 0.03293307720648721 1.448244380232507E-4 34-35 2.8964887604650138E-5 0.0 0.0 0.060898676188776917 1.448244380232507E-4 36-37 2.8964887604650138E-5 0.0 0.0 0.10728594368762412 1.448244380232507E-4 38-39 2.8964887604650138E-5 0.0 0.0 0.17335485231383108 1.448244380232507E-4 40-41 2.8964887604650138E-5 0.0 0.0 0.24447813382704953 1.448244380232507E-4 42-43 2.8964887604650138E-5 0.0 0.0 0.3210468142099422 1.448244380232507E-4 44-45 2.8964887604650138E-5 0.0 0.0 0.4127062010348575 1.448244380232507E-4 46-47 2.8964887604650138E-5 0.0 0.0 0.5184425232356328 1.7378932562790082E-4 48-49 2.8964887604650138E-5 0.0 0.0 0.6241933278802105 2.0275421323255098E-4 50-51 2.8964887604650138E-5 0.0 0.0 0.7376777575152298 2.0275421323255098E-4 52-53 2.8964887604650138E-5 0.0 0.0 0.8500470389774699 2.0275421323255098E-4 54-55 2.8964887604650138E-5 0.0 0.0 0.9708161378450587 2.0275421323255098E-4 56-57 2.8964887604650138E-5 0.0 0.0 1.1078200562150538 2.0275421323255098E-4 58-59 2.8964887604650138E-5 0.0 0.0 1.2512541796332814 2.0275421323255098E-4 60-61 2.8964887604650138E-5 0.0 0.0 1.4044639526180782 2.0275421323255098E-4 62-63 2.8964887604650138E-5 0.0 0.0 1.5547772368424102 2.317191008372011E-4 64-65 2.8964887604650138E-5 0.0 0.0 1.7194281404310439 2.317191008372011E-4 66-67 2.8964887604650138E-5 0.0 0.0 1.9025296774238396 2.317191008372011E-4 68-69 2.8964887604650138E-5 0.0 0.0 2.098955062714775 2.317191008372011E-4 70-71 2.8964887604650138E-5 0.0 0.0 2.3046202471515933 2.317191008372011E-4 72-73 2.8964887604650138E-5 0.0 0.0 2.5119364301818763 2.4620154463952617E-4 74-75 2.8964887604650138E-5 0.0 0.0 2.7302447880581244 2.6068398844185126E-4 76-77 2.8964887604650138E-5 0.0 0.0 2.9609356353853604 2.6068398844185126E-4 78-79 2.8964887604650138E-5 0.0 0.0 3.2094978183646656 2.6068398844185126E-4 80-81 2.8964887604650138E-5 0.0 0.0 3.4836504795426793 2.751664322441763E-4 82-83 2.8964887604650138E-5 0.0 0.0 3.761278927233251 3.186137636511515E-4 84-85 2.8964887604650138E-5 0.0 0.0 4.046322386150613 3.186137636511515E-4 86-87 2.8964887604650138E-5 0.0 0.0 4.338925680732789 3.186137636511515E-4 88-89 2.8964887604650138E-5 0.0 0.0 4.651876808857231 3.186137636511515E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7610 0.0 45.88911 1 GTATCAA 12190 0.0 39.210396 1 ATCAACG 15190 0.0 31.177217 3 TATCAAC 15375 0.0 30.995077 2 TCAACGC 15450 0.0 30.649887 4 CAACGCA 15665 0.0 30.138262 5 AACGCAG 16210 0.0 29.183157 6 ACGCAGA 18245 0.0 25.745916 7 CGCAGAG 18445 0.0 25.2865 8 GGACCGA 1565 0.0 23.065092 6 GAGTACT 12805 0.0 22.811377 12-13 GCAGAGT 20205 0.0 22.637236 9 GTACATG 10290 0.0 22.163218 1 AGGACCG 1765 0.0 21.79729 5 TACATGG 10245 0.0 21.379417 2 GTACTTT 13445 0.0 21.30161 14-15 ACATGGG 9925 0.0 21.106104 3 AGAGTAC 18240 0.0 20.519053 10-11 ACCGAGT 1780 0.0 20.27914 8 AGTACTT 13570 0.0 20.160374 12-13 >>END_MODULE