FastQCFastQC Report
Thu 26 May 2016
SRR938188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428077
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140890.41098843462384305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112990.32960169797819594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.2079591561099707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70850.046.5656551
GTATCAA121200.042.9721451
ATCAACG150250.034.2529073
TATCAAC152050.033.8777052
TCAACGC153000.033.6683354
CAACGCA155100.033.120485
AACGCAG160850.031.8999316
ACGCAGA179450.028.3988677
CGCAGAG181750.027.7762998
GCAGAGT196350.025.1053229
GTACATG101250.023.6977881
TACATGG101000.022.9810772
GAGTACT124900.022.93078412-13
ACATGGG99400.022.2510873
GTGGTAT32350.022.0744191
AGAGTAC177350.021.3738410-11
GTACTTT133500.021.23839814-15
AGTACTT133000.020.99812512-13
CAGAGTA188450.020.94722610-11
GGACCGA15250.019.6453656
Produced by FastQC (version 0.11.5)