##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3428077 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98247384758277 33.0 31.0 34.0 30.0 34.0 2 32.105546053953866 34.0 31.0 34.0 30.0 34.0 3 32.13343428400237 34.0 31.0 34.0 30.0 34.0 4 35.64500359822723 37.0 35.0 37.0 35.0 37.0 5 35.65204194654904 37.0 35.0 37.0 35.0 37.0 6 35.67773827717406 37.0 35.0 37.0 35.0 37.0 7 35.61361573850296 37.0 35.0 37.0 33.0 37.0 8 35.60931799373235 37.0 35.0 37.0 33.0 37.0 9 37.25352376857346 39.0 37.0 39.0 34.0 39.0 10-11 37.15616291582715 39.0 37.0 39.0 34.0 39.0 12-13 37.00550396038362 39.0 37.0 39.0 33.0 39.0 14-15 38.19463069236777 40.0 38.0 41.0 33.0 41.0 16-17 38.023524267395395 40.0 38.0 41.0 33.0 41.0 18-19 38.20597684941149 40.0 38.0 41.0 33.5 41.0 20-21 38.20690871296065 40.0 38.0 41.0 33.5 41.0 22-23 38.09610738031847 40.0 38.0 41.0 33.0 41.0 24-25 37.98271450728791 40.0 38.0 41.0 33.0 41.0 26-27 37.806616070759205 40.0 38.0 41.0 32.5 41.0 28-29 37.62465808090075 40.0 38.0 41.0 32.0 41.0 30-31 37.410163190616785 40.0 37.0 41.0 32.0 41.0 32-33 37.1201012987748 40.0 36.5 41.0 31.0 41.0 34-35 36.87924498195344 39.5 36.0 41.0 30.5 41.0 36-37 36.6176703440442 39.0 36.0 41.0 30.0 41.0 38-39 36.322698702508724 39.0 35.0 40.5 30.0 41.0 40-41 36.2146675818542 39.0 35.0 41.0 30.0 41.0 42-43 35.998517390362004 39.0 35.0 41.0 29.0 41.0 44-45 35.676628908860565 39.0 35.0 40.0 27.5 41.0 46-47 35.296760253634915 38.5 35.0 40.0 26.0 41.0 48-49 34.906569339020095 38.0 34.5 40.0 24.5 41.0 50-51 34.183572889407095 37.5 33.5 39.5 23.5 40.5 52-53 34.24870678225723 38.0 34.0 40.0 24.0 40.5 54-55 34.33197824903 38.0 34.0 40.0 23.0 41.0 56-57 34.02429277405379 38.0 33.5 40.0 22.0 41.0 58-59 33.628664846209695 38.0 33.0 40.0 20.0 41.0 60-61 33.13488276371855 37.0 32.5 40.0 17.5 41.0 62-63 32.594285513423415 36.5 31.5 39.5 13.5 41.0 64-65 32.065573497911515 36.0 31.0 39.0 8.0 40.5 66-67 31.499075866732284 35.5 30.5 39.0 6.5 40.0 68-69 30.81897489467127 35.0 29.5 38.0 2.0 40.0 70-71 30.236297492734266 35.0 29.0 37.5 2.0 40.0 72-73 29.531605766148196 34.0 28.5 37.0 2.0 39.0 74-75 28.845037903174287 34.0 27.5 36.0 2.0 39.0 76-77 28.083209916230004 33.5 26.0 36.0 2.0 38.5 78-79 27.301959670100757 33.0 25.5 35.0 2.0 37.5 80-81 26.56251653623883 32.5 23.5 35.0 2.0 37.0 82-83 25.805221994721823 32.0 21.0 35.0 2.0 36.0 84-85 25.055958486346718 31.0 18.5 34.5 2.0 36.0 86-87 24.281407477136597 31.0 11.5 34.0 2.0 35.0 88-89 23.3841745678408 30.5 4.5 34.0 2.0 35.0 90-91 22.462885314419715 29.5 2.0 34.0 2.0 35.0 92-93 21.50296857392643 29.0 2.0 34.0 2.0 35.0 94-95 20.390529150891304 28.0 2.0 34.0 2.0 35.0 96-97 19.089420978583618 25.5 2.0 33.5 2.0 35.0 98-99 17.48740635055747 22.0 2.0 33.0 2.0 34.5 100-101 13.511665140543808 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2437555619783538 1101 2 0.15888172010696167 1101 3 0.18555561852178926 1101 4 0.04827296423042782 1101 5 0.1912142914663022 1101 6 0.15338155599838643 1101 7 0.2296795140299892 1101 8 0.17356684416038348 1101 9 0.1742824226572921 1101 10-11 0.29588831774462676 1101 12-13 0.3582304568641703 1101 14-15 0.45445142419436735 1101 16-17 0.4832957468154433 1101 18-19 0.41540685045023906 1101 20-21 0.5069938871154918 1101 22-23 0.4999284820940062 1101 24-25 0.48345903625905606 1101 26-27 0.4805964103520495 1101 28-29 0.4325100008506624 1101 30-31 0.4195015894425751 1101 32-33 0.43572811312073867 1101 34-35 0.40683888530626433 1101 36-37 0.40512405346544256 1101 38-39 0.381146504500272 1101 40-41 0.5357293320365528 1101 42-43 0.5147669649115656 1101 44-45 0.5616124160477156 1101 46-47 0.5761275091055751 1101 48-49 0.48792231173558065 1101 50-51 0.4334666108964669 1101 52-53 0.5107195331565606 1101 54-55 0.42967179664241684 1101 56-57 0.3932676278168117 1101 58-59 0.5514962527346086 1101 60-61 0.4610786858131135 1101 62-63 0.4463328219952345 1101 64-65 0.4668819279677834 1101 66-67 0.3436871477701011 1101 68-69 0.38407723540581173 1101 70-71 0.2219485996560593 1101 72-73 0.34796260516035105 1101 74-75 0.3577929550635375 1101 76-77 0.14325065147976446 1101 78-79 0.054256942245842765 1101 80-81 0.237957491158717 1101 82-83 0.13535307193349055 1101 84-85 -0.05698712627076574 1101 86-87 -0.011682769137891569 1101 88-89 -0.024426884484245903 1101 90-91 -0.0485743877182685 1101 92-93 -0.1799666244885998 1101 94-95 -0.3278043107350612 1101 96-97 -0.5680263694884786 1101 98-99 -0.6254301671122029 1101 100-101 -0.7167684818321245 1102 1 0.249151101621095 1102 2 0.2874144146321598 1102 3 0.20298175767671012 1102 4 0.22166783234980159 1102 5 0.14565745707483302 1102 6 0.13952654370595496 1102 7 0.19192429613732997 1102 8 0.27266755168128753 1102 9 0.2548329426843452 1102 10-11 0.2790174767638405 1102 12-13 0.3029121047437897 1102 14-15 0.31720958807296995 1102 16-17 0.3280978169153883 1102 18-19 0.28551507440543844 1102 20-21 0.22282079981369662 1102 22-23 0.40152281202614404 1102 24-25 0.2502459637221506 1102 26-27 0.18498789341249733 1102 28-29 0.21659290600712922 1102 30-31 0.3333726365078604 1102 32-33 0.15051631690871403 1102 34-35 0.1825527689981996 1102 36-37 0.32553917113538233 1102 38-39 0.17062489549152815 1102 40-41 0.2620658007598706 1102 42-43 0.3906132699994842 1102 44-45 0.34254260505894507 1102 46-47 0.3637017439159962 1102 48-49 0.23582119150761116 1102 50-51 0.2394851929382753 1102 52-53 0.2559922615054404 1102 54-55 0.2298776659073667 1102 56-57 0.3412005142212138 1102 58-59 0.28765455791292993 1102 60-61 0.26890785198878575 1102 62-63 0.22130081970947657 1102 64-65 -0.02852071970683312 1102 66-67 -0.05995301470842307 1102 68-69 -0.09871374056839954 1102 70-71 -0.6039279186028672 1102 72-73 -0.41124972159818896 1102 74-75 -0.34550692585944276 1102 76-77 -0.7313223419809525 1102 78-79 -0.6737610253090303 1102 80-81 -0.6027919784292912 1102 82-83 -0.8190038933189392 1102 84-85 -0.7990400937529962 1102 86-87 -0.6914135248545854 1102 88-89 -0.750757111483896 1102 90-91 -0.8297426769640524 1102 92-93 -0.9414939850304691 1102 94-95 -0.9908268869419352 1102 96-97 -1.2485391163257837 1102 98-99 -1.2707943689975245 1102 100-101 -1.2958969112153618 1103 1 0.20615862425133002 1103 2 0.24228377378083366 1103 3 0.2651073358996001 1103 4 0.14887502273968778 1103 5 0.18358730600032658 1103 6 0.14632556692215815 1103 7 0.19947021000542264 1103 8 0.22559731341913647 1103 9 0.1622517641876584 1103 10-11 0.18907052414321868 1103 12-13 0.196431502884991 1103 14-15 0.2074831869197169 1103 16-17 0.24049419591693777 1103 18-19 0.27045439231183366 1103 20-21 0.2940158077215571 1103 22-23 0.23920855571806499 1103 24-25 0.22211303210377054 1103 26-27 0.25759740738827475 1103 28-29 0.34262510939200297 1103 30-31 0.3184192506489296 1103 32-33 0.34714903952874465 1103 34-35 0.3099831496675094 1103 36-37 0.31701453820794256 1103 38-39 0.23992959472390396 1103 40-41 0.2902282149039763 1103 42-43 0.2527967750857485 1103 44-45 0.29374247329977266 1103 46-47 0.3676809777351977 1103 48-49 0.42998494158811695 1103 50-51 0.466247343342296 1103 52-53 0.3340836605606512 1103 54-55 0.35351341872627273 1103 56-57 0.2690860042928591 1103 58-59 0.32220656614666154 1103 60-61 0.2079458003839889 1103 62-63 0.24669862969706458 1103 64-65 0.13007158191684454 1103 66-67 -0.06626056170079764 1103 68-69 -0.023000145011600637 1103 70-71 -0.07415575855377554 1103 72-73 0.024521058971082255 1103 74-75 -0.033707797312505505 1103 76-77 -0.10752456126422061 1103 78-79 -0.2592888749060158 1103 80-81 -0.08352461868976135 1103 82-83 -0.23481399601791608 1103 84-85 -0.3866986480045256 1103 86-87 -0.395275715566509 1103 88-89 -0.28967273511591785 1103 90-91 -0.30286655769901927 1103 92-93 -0.4802666814095389 1103 94-95 -0.6161579605866621 1103 96-97 -0.9800814676968095 1103 98-99 -1.0249138842019896 1103 100-101 -1.0605062586778633 1104 1 0.2169419477557497 1104 2 0.16853594030331465 1104 3 0.20052497619639098 1104 4 0.10483821920816894 1104 5 0.157544871073938 1104 6 0.1430030766651953 1104 7 0.13736975918399708 1104 8 0.1334385732443195 1104 9 0.23793247297018638 1104 10-11 0.27845879047011124 1104 12-13 0.2747896403929744 1104 14-15 0.27400318483750397 1104 16-17 0.293174333246526 1104 18-19 0.362639641205611 1104 20-21 0.3566131887755901 1104 22-23 0.3708294450504681 1104 24-25 0.32435841200931037 1104 26-27 0.3448508317999881 1104 28-29 0.23033999719854137 1104 30-31 0.2656593947216521 1104 32-33 0.2407760452068075 1104 34-35 0.2585237123855464 1104 36-37 0.35272837374823496 1104 38-39 0.25947744161454267 1104 40-41 0.3723102741590054 1104 42-43 0.30522770159097234 1104 44-45 0.47576798382835506 1104 46-47 0.32716805374844427 1104 48-49 0.20073984928533406 1104 50-51 0.27348762982120434 1104 52-53 0.2901089672944295 1104 54-55 0.2119550312761831 1104 56-57 0.26540846077645597 1104 58-59 0.2688672228874296 1104 60-61 0.2826851850372307 1104 62-63 0.31111549031999175 1104 64-65 0.2288749658342013 1104 66-67 0.09101162665023921 1104 68-69 0.20168443731538588 1104 70-71 0.2287208848785518 1104 72-73 0.14091309819590592 1104 74-75 0.035636172993111614 1104 76-77 0.046686567644457 1104 78-79 0.009264269590939733 1104 80-81 -0.0628317972027439 1104 82-83 -0.08912765738005035 1104 84-85 -0.17997711454393084 1104 86-87 0.05454857155823234 1104 88-89 -0.28297059968705796 1104 90-91 -0.3943521880970451 1104 92-93 -0.3503063636628525 1104 94-95 -0.6261719539187851 1104 96-97 -1.2109282447032506 1104 98-99 -1.0798277299031582 1104 100-101 -1.168518800429096 1105 1 0.23779390182573934 1105 2 0.2367004433528237 1105 3 0.288589305475071 1105 4 0.13126719464174386 1105 5 0.2280726573698928 1105 6 0.16186545698479904 1105 7 0.24458813118985745 1105 8 0.2771946126323712 1105 9 0.349564760271285 1105 10-11 0.41015595221327317 1105 12-13 0.37173427223734024 1105 14-15 0.3846014335001584 1105 16-17 0.3808926200706111 1105 18-19 0.3809121547495167 1105 20-21 0.42705865540137466 1105 22-23 0.4334970657530448 1105 24-25 0.4337021034468407 1105 26-27 0.4262111507205617 1105 28-29 0.4043491968305588 1105 30-31 0.4692525665503382 1105 32-33 0.3888552301875521 1105 34-35 0.37302165098652296 1105 36-37 0.4117453078856883 1105 38-39 0.4983086113307209 1105 40-41 0.314968490793305 1105 42-43 0.3105093806941781 1105 44-45 0.32748027385527223 1105 46-47 0.5253179055321482 1105 48-49 0.5417894250800828 1105 50-51 0.549975823626788 1105 52-53 0.5891368046994572 1105 54-55 0.581717953351891 1105 56-57 0.4649154967913134 1105 58-59 0.49208586747498373 1105 60-61 0.5433545093753764 1105 62-63 0.4983499446455184 1105 64-65 0.3790613435642811 1105 66-67 0.4218297124453265 1105 68-69 0.3256882534590382 1105 70-71 0.10560391326112395 1105 72-73 0.19307855528132478 1105 74-75 0.16134553276686603 1105 76-77 0.010753350158342556 1105 78-79 0.0252690397705031 1105 80-81 0.07724057323576972 1105 82-83 -0.13790655955729392 1105 84-85 -0.22111964069473444 1105 86-87 -0.15777471032530954 1105 88-89 -0.3194910944228262 1105 90-91 -0.5846600009697127 1105 92-93 -0.6784307972040295 1105 94-95 -0.7666094475301506 1105 96-97 -0.6403368105828413 1105 98-99 -0.7095582147578376 1105 100-101 -1.0064963805851495 1106 1 0.39784989092520107 1106 2 0.3246206712730455 1106 3 0.3217863821504068 1106 4 0.3407397575056095 1106 5 0.4292444646286597 1106 6 0.4185502086260726 1106 7 0.5128769953048149 1106 8 0.33946489629396126 1106 9 0.5444375461005961 1106 10-11 0.5410973618503334 1106 12-13 0.6346779115288044 1106 14-15 0.6613515883372614 1106 16-17 0.1905931031623993 1106 18-19 0.6303844698740022 1106 20-21 0.7057706583742416 1106 22-23 0.7381961862782589 1106 24-25 0.6537063150162723 1106 26-27 0.6098432577439752 1106 28-29 0.5716423963846253 1106 30-31 0.5167055580033306 1106 32-33 0.5536202493873574 1106 34-35 0.43938483691492536 1106 36-37 0.4933040840531646 1106 38-39 0.5526301770435893 1106 40-41 0.41415070310594615 1106 42-43 0.3183349721719466 1106 44-45 0.5641199369297212 1106 46-47 0.6742216587837362 1106 48-49 0.7750945161243052 1106 50-51 0.6503593241842012 1106 52-53 0.5947931901819317 1106 54-55 0.6306286433060606 1106 56-57 0.5523162152355141 1106 58-59 0.6612809622352955 1106 60-61 0.9371835692044357 1106 62-63 0.7485991704599613 1106 64-65 0.5343765919230066 1106 66-67 0.6158517612499814 1106 68-69 0.543615170342477 1106 70-71 0.38223218032416995 1106 72-73 0.303996677416837 1106 74-75 0.3910447771617669 1106 76-77 -0.062334968929974366 1106 78-79 0.015997641281714436 1106 80-81 0.0756775854988696 1106 82-83 0.12551150064337335 1106 84-85 0.08523289244040555 1106 86-87 0.030414562559617053 1106 88-89 -0.2229619231110469 1106 90-91 -0.3858258624834008 1106 92-93 -0.32258439561849883 1106 94-95 -0.6666837693435994 1106 96-97 -0.749809868925464 1106 98-99 -1.0621570008348833 1106 100-101 -1.1349544506953944 1107 1 0.018163177505357453 1107 2 0.056871944903633675 1107 3 0.037376023227302824 1107 4 0.05852207513800067 1107 5 0.014429161657432132 1107 6 0.024837907267588832 1107 7 0.05227140066623548 1107 8 0.03221668889582929 1107 9 0.026991507215271326 1107 10-11 0.11236905201258196 1107 12-13 0.04783680827090819 1107 14-15 0.03348630852295997 1107 16-17 0.04207926484346558 1107 18-19 0.026675701547546282 1107 20-21 0.027418808658076443 1107 22-23 0.19394990282657432 1107 24-25 0.10572691643717036 1107 26-27 0.03337480698766626 1107 28-29 0.13335412293893256 1107 30-31 -0.03435334477970997 1107 32-33 0.04910080581244358 1107 34-35 0.10051663365391761 1107 36-37 0.17983105866109383 1107 38-39 0.06311174944420372 1107 40-41 0.07804201752199447 1107 42-43 0.0052223892692566665 1107 44-45 0.0022155411422630777 1107 46-47 0.04707488418567607 1107 48-49 0.07751903646486369 1107 50-51 -0.038089857818199846 1107 52-53 -0.005214955928167342 1107 54-55 0.004164952074752648 1107 56-57 -0.08349866120904892 1107 58-59 0.015533048646304337 1107 60-61 0.025219862413145222 1107 62-63 -0.015714277211806404 1107 64-65 -0.09194394744101686 1107 66-67 0.04153049697369582 1107 68-69 -0.0600209236984135 1107 70-71 -0.25019977219773537 1107 72-73 -0.19511265070298478 1107 74-75 -0.3180443595885407 1107 76-77 -0.36238549311843116 1107 78-79 -0.47598182685967316 1107 80-81 -0.4625415833050077 1107 82-83 -0.47809484636627175 1107 84-85 -0.5475385423550172 1107 86-87 -0.5414740352660132 1107 88-89 -0.5193706784403425 1107 90-91 -0.6461659305446119 1107 92-93 -0.8250817733032818 1107 94-95 -0.9778300277162479 1107 96-97 -1.040158375990611 1107 98-99 -1.1723405537470732 1107 100-101 -1.28215301258661 1108 1 -0.011624631728114565 1108 2 -0.011228188933053218 1108 3 0.10053738345833096 1108 4 0.11364538338528973 1108 5 0.07886000764303702 1108 6 0.04250375698980946 1108 7 0.016414723682217414 1108 8 0.04068321083816073 1108 9 0.06209360305182088 1108 10-11 0.08096435195265883 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3729.0 7 4889.0 8 5972.0 9 7839.0 10 9877.0 11 12567.0 12 15892.0 13 19983.0 14 23366.0 15 28130.0 16 33153.0 17 41623.0 18 40959.0 19 35353.0 20 36257.0 21 38495.0 22 42461.0 23 47368.0 24 53435.0 25 59860.0 26 67603.0 27 77969.0 28 89258.0 29 103484.0 30 121493.0 31 144784.0 32 175345.0 33 217738.0 34 276183.0 35 361383.0 36 456888.0 37 489492.0 38 251204.0 39 14158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.756564744786324 18.96226056829701 14.903263311336435 23.37791137558023 2 15.186334896144652 19.825415374701137 40.81473798005081 24.173511749103408 3 18.73812942704263 24.081154474846294 30.031684076596875 27.149032021514202 4 12.604757537343513 16.869278716680007 37.628650375917886 32.8973133700586 5 13.530622826548882 37.14982922205575 34.63600594142402 14.683542009971351 6 31.632252203589577 36.60945927144459 17.49385309870468 14.264435426261151 7 28.141428260874648 32.89941492308447 20.809208357974505 18.14994845806637 8 25.816506471772527 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22.051315444258908 32-33 21.62077421075252 26.644222685225277 29.460347010843414 22.274656093178788 34-35 22.215397884977783 26.768260885053113 29.19960500275342 21.81673622721568 36-37 22.291260985063424 26.357597563946904 29.217557065250315 22.133584385739354 38-39 21.784730098338684 26.760035980272722 29.21053537875308 22.244698542635515 40-41 22.247265522613986 26.50986583445853 29.203611372343673 22.03925727058381 42-43 22.034628528145255 26.466327051102144 29.101843074841664 22.39720134591093 44-45 21.966684584456114 26.779566249421467 29.06317975978489 22.19056940633753 46-47 22.18745471756676 27.043964217374356 28.485997668406142 22.282583396652736 48-49 22.160990357002625 26.88252358293376 28.580127111243275 22.37635894882034 50-51 22.359281590920567 27.2004904425862 28.222951160335676 22.21727680615756 52-53 22.431166674228777 27.099838978096674 28.116814045159956 22.352180302514594 54-55 22.10717341841458 27.18859773544729 28.34486448056428 22.35936436557385 56-57 22.19265473618605 27.290770159092524 28.255788710744785 22.260786393976637 58-59 22.276317554044624 26.888925954479078 28.187418216745268 22.64733827473103 60-61 22.122271769450823 27.204769801876903 28.335573999017743 22.337384429654527 62-63 22.032634659890117 27.250905598167602 28.390887441695938 22.325572300246343 64-65 21.79551268470254 27.16667454590767 28.33995189577457 22.697860873615223 66-67 21.898084251943114 27.067833183994956 28.268376609363628 22.76570595469831 68-69 22.030020736568517 26.918512057800847 28.42834966805144 22.623117537579194 70-71 22.068063965639674 27.048878418178095 28.117691593528487 22.765366022653744 72-73 22.119830933496463 27.06448914644881 27.92323246002153 22.892447460033203 74-75 21.92584384494633 27.377636244833447 27.979412287431852 22.717107622788372 76-77 22.169115340793873 27.015987500601774 27.978519941704487 22.836377216899866 78-79 22.196787825879927 27.1914834145237 28.000070608776028 22.611658150820347 80-81 22.245413995770917 27.38565735912566 27.70228415383776 22.666644491265664 82-83 22.38601056149097 27.278789457882564 27.70313976629805 22.632060214328416 84-85 22.34617222175621 27.176696706334592 27.65202612758167 22.825104944327535 86-87 22.415315640873278 27.148568316530948 27.561976592545346 22.874139450050425 88-89 22.483650458038205 27.243053241660014 27.468471060044624 22.804825240257156 90-91 22.459584700270593 27.19871262106776 27.466809488126408 22.874893190535236 92-93 22.568127407342097 26.748631554608558 27.601060744976724 23.08218029307262 94-95 22.39630498283743 26.78010193029995 27.62729756255915 23.19629552430347 96-97 22.547370621107422 26.60710699431833 27.608098893323273 23.237423491250972 98-99 22.533683462720095 26.6641215447489 27.271477530834794 23.530717461696206 100-101 23.26932092517865 25.545843428833482 27.44131465684062 23.743520989147246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1079.0 1 731.5 2 507.5 3 671.0 4 575.0 5 547.5 6 1063.0 7 2324.0 8 3879.5 9 4514.5 10 3895.0 11 3219.5 12 3235.0 13 3627.5 14 4003.5 15 4281.0 16 4531.0 17 4752.0 18 5175.0 19 5764.0 20 6484.5 21 7581.5 22 9017.0 23 11070.5 24 13978.0 25 17601.5 26 22169.0 27 27521.5 28 32349.0 29 37383.5 30 43717.5 31 50940.0 32 58714.0 33 64529.5 34 68822.0 35 72875.5 36 78900.0 37 86183.0 38 90728.0 39 93465.5 40 97335.5 41 104442.0 42 109970.0 43 115761.5 44 122693.0 45 127878.0 46 129509.0 47 128924.0 48 128324.0 49 125161.0 50 122049.0 51 118049.0 52 113585.0 53 110884.5 54 109127.5 55 102930.5 56 93723.0 57 85548.5 58 78325.0 59 70206.0 60 58467.5 61 48295.0 62 40067.5 63 31123.0 64 24503.5 65 19842.0 66 15130.0 67 11809.5 68 9030.0 69 6423.0 70 4806.0 71 3408.5 72 2440.0 73 1902.0 74 1446.0 75 1153.0 76 812.0 77 448.0 78 241.0 79 148.5 80 82.0 81 52.5 82 46.0 83 32.5 84 19.5 85 15.5 86 10.0 87 7.0 88 6.5 89 3.5 90 2.5 91 1.5 92 2.0 93 3.5 94 2.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14197464059296216 2 0.051719958449008 3 0.15233030063210365 4 0.1300145825195875 5 0.19471557960920946 6 0.19955794458525875 7 0.16396947909863166 8 0.0686682358651804 9 0.11872545453325582 10-11 0.10113541790339016 12-13 0.15027375406095023 14-15 0.1360091969929497 16-17 0.133690112561649 18-19 0.13296084072790662 20-21 0.13411309022521956 22-23 0.19662627181361447 24-25 0.2154414851241673 26-27 0.1485672579699931 28-29 0.12820598837190647 30-31 0.10021653539287478 32-33 0.12524514472691248 34-35 0.09460114227305863 36-37 0.0883439899395492 38-39 0.051194882728713496 40-41 0.05866262630623524 42-43 0.06255693789841944 44-45 0.03835969845484801 46-47 0.04858408956391586 48-49 0.040649612012799013 50-51 0.05204083805585463 52-53 0.026355884071448805 54-55 0.04973633906122878 56-57 0.025932906407878237 58-59 0.05013014585144967 60-61 0.03698866740741238 62-63 0.05106361379863988 64-65 0.039409849895437006 66-67 0.037790866424528974 68-69 0.0654886106700637 70-71 0.05250757202944975 72-73 0.050553123515020226 74-75 0.04288118382405063 76-77 0.020769661824982345 78-79 0.021615617152123477 80-81 0.02497026758733832 82-83 0.021003028811779897 84-85 0.019384045340871865 86-87 0.02437226468366959 88-89 0.06465724077959743 90-91 0.10953662942810212 92-93 0.0801761454016348 94-95 0.07581509983585549 96-97 0.06782228053803926 98-99 0.055862222464664595 100-101 0.13191068928731764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3428077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.36736139909537 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61832985271886 33.450039515342596 2 14.70871297761934 13.93425846652919 3 5.895175735289471 8.377167586939027 4 2.930840504586257 5.553047255353772 5 1.6242424046112245 3.8468038489477783 6 0.9765506595948733 2.77539768105332 7 0.6942956428504801 2.3020866842901158 8 0.47601053359742956 1.8037890379748542 9 0.32911323406781806 1.4030302949383864 >10 1.570139354609799 13.000079907530932 >50 0.10306133384116711 3.3597454127664066 >100 0.06623996247908781 5.98125109073199 >500 0.004693839954665767 1.536684615241956 >1k 0.0024086810238260364 1.7253474032326355 >5k 6.176105189297529E-5 0.20855590548256878 >10k+ 1.2352210378595058E-4 0.7427152936444741 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14089 0.41098843462384305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11299 0.32960169797819594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7129 0.2079591561099707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.9170873349694305E-5 0.0 9 0.0 0.0 0.0 8.751262004908292E-5 0.0 10-11 0.0 0.0 0.0 1.0209805672393006E-4 0.0 12-13 0.0 0.0 0.0 1.3126893007362438E-4 2.9170873349694305E-5 14-15 0.0 0.0 0.0 1.7502524009816584E-4 4.375631002454146E-5 16-17 0.0 0.0 0.0 2.0419611344786012E-4 5.834174669938861E-5 18-19 0.0 0.0 0.0 2.3336698679755444E-4 1.1668349339877722E-4 20-21 0.0 0.0 0.0 2.6253786014724875E-4 1.6043980342331866E-4 22-23 0.0 0.0 0.0 4.2297766357056736E-4 1.7502524009816584E-4 24-25 0.0 0.0 0.0 5.542465936441917E-4 1.7502524009816584E-4 26-27 0.0 0.0 0.0 0.0011084931872883835 1.7502524009816584E-4 28-29 0.0 0.0 0.0 0.004156849452331439 1.7502524009816584E-4 30-31 0.0 0.0 0.0 0.012747671653816411 1.7502524009816584E-4 32-33 0.0 0.0 0.0 0.032525523784909145 2.0419611344786012E-4 34-35 0.0 0.0 0.0 0.06160888451455437 2.0419611344786012E-4 36-37 0.0 0.0 0.0 0.1087927721576849 2.0419611344786012E-4 38-39 0.0 0.0 0.0 0.17795691286981008 2.0419611344786012E-4 40-41 0.0 0.0 0.0 0.24879837879954272 2.0419611344786012E-4 42-43 0.0 0.0 0.0 0.3218130747938276 2.0419611344786012E-4 44-45 0.0 0.0 0.0 0.4139055219588125 2.0419611344786012E-4 46-47 0.0 0.0 0.0 0.5194019854279819 2.0419611344786012E-4 48-49 0.0 0.0 0.0 0.6278592925421453 2.6253786014724875E-4 50-51 0.0 0.0 0.0 0.7420486762695238 2.6253786014724875E-4 52-53 0.0 0.0 0.0 0.850228860086865 2.6253786014724875E-4 54-55 0.0 0.0 0.0 0.971798474771716 2.9170873349694304E-4 56-57 0.0 0.0 0.0 1.1099517309558682 2.9170873349694304E-4 58-59 0.0 0.0 0.0 1.2561269773111863 3.062941701717902E-4 60-61 0.0 0.0 0.0 1.4095074293838792 3.354650435214845E-4 62-63 0.0 0.0 0.0 1.5608605057587681 3.5005048019633167E-4 64-65 0.0 0.0 0.0 1.7224817295527493 3.5005048019633167E-4 66-67 0.0 0.0 0.0 1.901722160849946 3.5005048019633167E-4 68-69 0.0 0.0 0.0 2.0948187569882473 3.5005048019633167E-4 70-71 0.0 0.0 0.0 2.3026612296048192 4.0839222689572025E-4 72-73 2.9170873349694305E-5 0.0 0.0 2.5106787274614892 4.521485369202617E-4 74-75 2.9170873349694305E-5 0.0 0.0 2.7264265067558284 5.104902836196503E-4 76-77 2.9170873349694305E-5 0.0 0.0 2.957284798445309 5.250757202944975E-4 78-79 2.9170873349694305E-5 0.0 0.0 3.2082855781827537 5.542465936441917E-4 80-81 2.9170873349694305E-5 0.0 0.0 3.468927331562272 5.542465936441917E-4 82-83 2.9170873349694305E-5 0.0 0.0 3.7368034615325154 5.834174669938861E-4 84-85 2.9170873349694305E-5 0.0 0.0 4.011286794316463 5.834174669938861E-4 86-87 2.9170873349694305E-5 0.0 0.0 4.293704604651529 5.980029036687332E-4 88-89 2.9170873349694305E-5 0.0 0.0 4.591276683691761 6.125883403435804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7085 0.0 46.565655 1 GTATCAA 12120 0.0 42.972145 1 ATCAACG 15025 0.0 34.252907 3 TATCAAC 15205 0.0 33.877705 2 TCAACGC 15300 0.0 33.668335 4 CAACGCA 15510 0.0 33.12048 5 AACGCAG 16085 0.0 31.899931 6 ACGCAGA 17945 0.0 28.398867 7 CGCAGAG 18175 0.0 27.776299 8 GCAGAGT 19635 0.0 25.105322 9 GTACATG 10125 0.0 23.697788 1 TACATGG 10100 0.0 22.981077 2 GAGTACT 12490 0.0 22.930784 12-13 ACATGGG 9940 0.0 22.251087 3 GTGGTAT 3235 0.0 22.074419 1 AGAGTAC 17735 0.0 21.37384 10-11 GTACTTT 13350 0.0 21.238398 14-15 AGTACTT 13300 0.0 20.998125 12-13 CAGAGTA 18845 0.0 20.947226 10-11 GGACCGA 1525 0.0 19.645365 6 >>END_MODULE