FastQCFastQC Report
Thu 26 May 2016
SRR938188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428077
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139430.4067294871147877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114970.3353775309014354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74690.21787725304886676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.046.5380061
GTATCAA128300.040.4373361
ATCAACG158100.032.5363663
TATCAAC159900.032.2639242
TCAACGC159800.032.1850744
CAACGCA162150.031.718625
AACGCAG168200.030.6624326
ACGCAGA188750.027.2478127
CGCAGAG188800.027.0896768
GCAGAGT207300.024.2597759
GAGTACT129150.023.82846512-13
GTACATG104250.022.4676361
GTACTTT136800.022.14879214-15
TACATGG102550.021.9109552
ACATGGG100550.021.1153343
AGAGTAC185100.021.02606610-11
CAGAGTA200100.020.94513110-11
AGTACTT136100.020.69245512-13
GTGTAGC17000.020.6808951
GGACCGA16050.020.416296
Produced by FastQC (version 0.11.5)