##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3428077 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56255475008292 34.0 31.0 34.0 31.0 34.0 2 32.7745739083457 34.0 31.0 34.0 31.0 34.0 3 32.829845420625034 34.0 31.0 34.0 31.0 34.0 4 36.27266248686946 37.0 37.0 37.0 35.0 37.0 5 36.24579844618426 37.0 37.0 37.0 35.0 37.0 6 36.2765372539765 37.0 37.0 37.0 35.0 37.0 7 36.23750487518221 37.0 37.0 37.0 35.0 37.0 8 36.226114524265355 37.0 37.0 37.0 35.0 37.0 9 37.92775366480975 39.0 38.0 39.0 35.0 39.0 10-11 37.90496085706359 39.0 38.0 39.0 35.0 39.0 12-13 37.82796258660468 39.0 38.0 39.0 35.0 39.0 14-15 39.13606505921541 40.0 38.5 41.0 36.0 41.0 16-17 39.03246368153341 40.0 38.0 41.0 36.0 41.0 18-19 39.273258301957625 40.5 39.0 41.0 36.0 41.0 20-21 39.25526249847947 41.0 39.0 41.0 36.0 41.0 22-23 39.16143817656371 40.5 39.0 41.0 36.0 41.0 24-25 39.07758635526565 40.5 39.0 41.0 35.5 41.0 26-27 38.91725565090866 40.0 38.0 41.0 35.0 41.0 28-29 38.807402955067815 40.0 38.0 41.0 35.0 41.0 30-31 38.58182940464873 40.0 38.0 41.0 34.5 41.0 32-33 38.38076463860059 40.0 38.0 41.0 34.0 41.0 34-35 38.2297589873273 40.0 38.0 41.0 34.0 41.0 36-37 38.227004381756885 40.0 38.0 41.0 34.0 41.0 38-39 38.088638324051644 40.0 38.0 41.0 34.0 41.0 40-41 37.925668530782715 40.0 38.0 41.0 33.0 41.0 42-43 37.708618126138944 40.0 37.0 41.0 33.0 41.0 44-45 37.44800568948714 40.0 37.0 41.0 33.0 41.0 46-47 37.0733462229699 40.0 37.0 41.0 31.5 41.0 48-49 36.70466576450879 40.0 36.0 41.0 31.0 41.0 50-51 36.3490351879494 39.0 36.0 41.0 30.0 41.0 52-53 35.858143793152834 39.0 35.0 40.0 29.0 41.0 54-55 35.563511700583156 38.5 35.0 40.0 28.0 41.0 56-57 35.241998502367366 38.0 35.0 40.0 27.0 41.0 58-59 34.83912219591333 38.0 34.0 40.0 26.5 41.0 60-61 34.38990926983262 37.5 34.0 40.0 26.0 41.0 62-63 33.94551026712644 37.0 33.0 40.0 25.0 41.0 64-65 33.48701414816529 36.5 33.0 39.0 23.0 40.5 66-67 32.95682725329682 36.0 32.0 39.0 22.0 40.0 68-69 32.401459914698535 35.0 31.5 38.5 20.5 40.0 70-71 31.816014780298108 35.0 31.0 38.0 18.5 40.0 72-73 31.247568388924755 35.0 30.5 37.0 15.5 39.0 74-75 30.657172519753786 34.0 30.0 36.5 10.0 39.0 76-77 29.266440047875236 32.5 28.5 35.0 8.0 37.5 78-79 29.95747455497645 34.0 29.5 36.0 7.0 38.0 80-81 29.635989506653438 34.0 29.0 35.0 4.5 37.0 82-83 29.077852831193695 34.0 29.0 35.0 2.0 37.0 84-85 28.4883536746695 34.0 28.0 35.0 2.0 36.0 86-87 27.85684656441498 33.0 26.5 35.0 2.0 36.0 88-89 27.138152964475417 33.0 25.5 35.0 2.0 35.0 90-91 26.605241218327357 32.0 25.0 35.0 2.0 35.0 92-93 25.79943420174051 32.0 23.5 34.0 2.0 35.0 94-95 24.9931638058305 31.5 19.5 34.0 2.0 35.0 96-97 23.96110209893185 31.0 7.5 34.0 2.0 35.0 98-99 22.517689801016722 31.0 2.0 34.0 2.0 35.0 100-101 18.035874194191088 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09550785182587873 1101 2 0.08700508855099542 1101 3 0.12401693143379333 1101 4 0.09201815081699039 1101 5 0.08759714007375408 1101 6 0.06429305055370804 1101 7 0.08130966131520267 1101 8 0.054221682317674436 1101 9 6.407268806967181E-4 1101 10-11 0.08448423528678717 1101 12-13 0.24119828828104772 1101 14-15 0.22195206992912375 1101 16-17 0.17794487985566576 1101 18-19 0.21771696388729112 1101 20-21 0.2540731394721547 1101 22-23 0.18749322091667153 1101 24-25 0.21103162373687923 1101 26-27 0.231464898778043 1101 28-29 0.17741491622162897 1101 30-31 0.21238853679981418 1101 32-33 0.22996876725078863 1101 34-35 0.23014515737799712 1101 36-37 0.28842844573457427 1101 38-39 0.293274832772461 1101 40-41 0.18397124813310484 1101 42-43 0.17116217452520743 1101 44-45 0.27366245172472503 1101 46-47 0.3133959457913704 1101 48-49 0.29550163399628815 1101 50-51 0.16654991740073655 1101 52-53 -0.028034526117671987 1101 54-55 0.03468895533694649 1101 56-57 0.11386627107385294 1101 58-59 0.2287110454242125 1101 60-61 0.08126335101309934 1101 62-63 0.02196106419326327 1101 64-65 0.04611936247749071 1101 66-67 -0.11152612715684995 1101 68-69 -0.09292898738416255 1101 70-71 -0.0038357119973291276 1101 72-73 -0.024945591964581126 1101 74-75 -0.0388113053326542 1101 76-77 -0.23612564156537985 1101 78-79 -0.0821578944877075 1101 80-81 -0.21987974734483018 1101 82-83 -0.20979864736744602 1101 84-85 -0.20565980837728048 1101 86-87 -0.42119124534809416 1101 88-89 -0.5542484082249892 1101 90-91 -0.5188772616233024 1101 92-93 -0.5749384627494436 1101 94-95 -0.5357028977048337 1101 96-97 -0.6853247590433149 1101 98-99 -0.6455985596827993 1101 100-101 -0.7527996225438685 1102 1 0.1408093136267965 1102 2 0.08290061716310504 1102 3 0.05634297970706825 1102 4 0.04644947867375038 1102 5 0.11196644349201534 1102 6 0.0247594794967938 1102 7 0.03393863765637661 1102 8 0.12215584935157864 1102 9 0.19939830102403278 1102 10-11 0.15823708778140855 1102 12-13 0.13471658110505302 1102 14-15 0.1980710737715512 1102 16-17 0.20524903097855685 1102 18-19 0.28171104285908655 1102 20-21 0.20878087883284735 1102 22-23 0.2233274945441579 1102 24-25 0.1901306739725257 1102 26-27 0.17485515555502218 1102 28-29 0.11510002233899996 1102 30-31 0.15022947077690674 1102 32-33 0.07456206245580432 1102 34-35 0.07608860198080691 1102 36-37 0.24324584572306662 1102 38-39 0.2517345475579802 1102 40-41 0.1861684424783192 1102 42-43 0.13567463940879065 1102 44-45 0.02619209856693061 1102 46-47 -0.016493032239374372 1102 48-49 0.06819118734177465 1102 50-51 0.024246796700325035 1102 52-53 -0.08091580799552389 1102 54-55 0.026390960383054107 1102 56-57 0.023705379884852107 1102 58-59 0.06022852144334934 1102 60-61 0.028929565154804493 1102 62-63 0.18882122429348414 1102 64-65 0.17218786754700943 1102 66-67 0.10073837556879539 1102 68-69 0.029657066345244232 1102 70-71 -0.015085019892413953 1102 72-73 -0.09613062086752677 1102 74-75 -0.11312828050727575 1102 76-77 -0.21475667014225053 1102 78-79 -0.1766405336176149 1102 80-81 -0.24712207311868184 1102 82-83 -0.11620787521779974 1102 84-85 -0.44461494383613953 1102 86-87 -0.4650878350532288 1102 88-89 -0.5205934646991359 1102 90-91 -0.6705927939912115 1102 92-93 -0.7284609203097574 1102 94-95 -0.508906317093242 1102 96-97 -0.6843765551262031 1102 98-99 -0.6357731180943027 1102 100-101 -1.0802903796810917 1103 1 0.051051704338100024 1103 2 0.1249791866326575 1103 3 0.04301123355836722 1103 4 0.06424230894683092 1103 5 0.055881507208034975 1103 6 0.03256322687197155 1103 7 0.01998599455550476 1103 8 0.03156315382119601 1103 9 0.1464281305868127 1103 10-11 0.09118555907969039 1103 12-13 0.12311734912233163 1103 14-15 0.10677225915051736 1103 16-17 0.1063860082042325 1103 18-19 0.12498707660967057 1103 20-21 0.07239279969147105 1103 22-23 0.16760657925738087 1103 24-25 0.2199274783892804 1103 26-27 0.19600914495401156 1103 28-29 0.2853357041377862 1103 30-31 0.2334378267597046 1103 32-33 0.1461056697320302 1103 34-35 0.058810027592464564 1103 36-37 0.09193930617631452 1103 38-39 0.06278865015978852 1103 40-41 0.125873048737418 1103 42-43 0.09761758724407343 1103 44-45 0.20632886926373573 1103 46-47 0.1663513934616887 1103 48-49 0.1990582729593271 1103 50-51 0.04683529996673741 1103 52-53 0.08691702275734059 1103 54-55 0.12319549809150487 1103 56-57 0.10769247772682178 1103 58-59 0.07377450727903323 1103 60-61 0.008394456808787254 1103 62-63 -0.07330141071658147 1103 64-65 -0.07425943770478227 1103 66-67 -0.010322903634154557 1103 68-69 0.03693279932367233 1103 70-71 -0.09897283469900842 1103 72-73 -0.339271851438923 1103 74-75 -0.5277812570681135 1103 76-77 -0.1466033517353047 1103 78-79 -0.35243102564921713 1103 80-81 -0.24794954934090185 1103 82-83 -0.2881631190302265 1103 84-85 -0.4297665359278078 1103 86-87 -0.482137856870299 1103 88-89 -0.5441607878198589 1103 90-91 -0.5948418035043375 1103 92-93 -0.48058031073006546 1103 94-95 -0.5348831941247028 1103 96-97 -0.7883451953951415 1103 98-99 -0.9021081760629919 1103 100-101 -1.336809621287717 1104 1 0.12053485744581849 1104 2 0.07897813994719627 1104 3 0.0707629275906001 1104 4 0.041362542200459984 1104 5 0.007555102224280574 1104 6 0.0648518087728931 1104 7 0.04223850054354017 1104 8 0.0478112184682189 1104 9 0.10996810587933936 1104 10-11 0.1266865069949148 1104 12-13 0.102998241743542 1104 14-15 0.17555079030191933 1104 16-17 0.1308384492050223 1104 18-19 0.13284303891128957 1104 20-21 0.14887973916797392 1104 22-23 0.07071629735375495 1104 24-25 0.06069946616890576 1104 26-27 0.16529843320886073 1104 28-29 7.431489214653197E-4 1104 30-31 -0.01816364144806215 1104 32-33 0.10170678885220497 1104 34-35 0.10763302603745473 1104 36-37 0.06632837161359362 1104 38-39 0.1913723427079148 1104 40-41 0.19526907427700735 1104 42-43 0.15527732248210668 1104 44-45 0.07930211893535954 1104 46-47 0.09910045730940453 1104 48-49 0.16253243350133317 1104 50-51 0.1904470787672139 1104 52-53 0.22566697741347497 1104 54-55 0.10659093687912247 1104 56-57 0.16594204398933243 1104 58-59 0.26287866364410917 1104 60-61 0.14748498355483264 1104 62-63 0.24960942559549437 1104 64-65 0.16149939049244466 1104 66-67 0.06274248668820803 1104 68-69 0.2813821574229749 1104 70-71 -0.018059853249436486 1104 72-73 -0.17947382959554403 1104 74-75 -0.16019913993652324 1104 76-77 -0.29957444029684765 1104 78-79 -0.3040519399989208 1104 80-81 -0.3194233099909347 1104 82-83 -0.3253592778735488 1104 84-85 -0.3835466359527544 1104 86-87 -0.5273929068293697 1104 88-89 -0.5327732002316345 1104 90-91 -0.5052027868267501 1104 92-93 -0.5251518109994322 1104 94-95 -0.39378108379012033 1104 96-97 -0.5883005395095857 1104 98-99 -0.5579195944795963 1104 100-101 -1.160343628991317 1105 1 0.13907531879033286 1105 2 0.16267380249106367 1105 3 0.131186110663279 1105 4 0.09266732260184085 1105 5 0.11383797387237848 1105 6 0.11160665561605754 1105 7 0.10372693285810186 1105 8 0.0932375021576135 1105 9 0.07308889364042415 1105 10-11 0.15330165572230214 1105 12-13 0.15321392200522155 1105 14-15 0.19385901204712042 1105 16-17 0.20938504363753196 1105 18-19 0.17492597189027492 1105 20-21 0.14084403096667586 1105 22-23 0.21917682411786643 1105 24-25 0.21428343836557673 1105 26-27 0.27008019749384715 1105 28-29 0.14763564951774555 1105 30-31 0.17034349678492333 1105 32-33 0.20814557722659544 1105 34-35 0.21023672633388202 1105 36-37 0.22302141055870095 1105 38-39 0.23789794835035138 1105 40-41 0.1530581641948885 1105 42-43 0.1522215454539264 1105 44-45 0.06962001733053569 1105 46-47 0.011066520889080778 1105 48-49 0.08613405536238616 1105 50-51 0.03786947816752928 1105 52-53 -0.024505771232135487 1105 54-55 0.03858398604604929 1105 56-57 0.030054620227026874 1105 58-59 0.09386942993938163 1105 60-61 -0.1004585920354728 1105 62-63 -0.07720811503621405 1105 64-65 1.5174662042483078E-4 1105 66-67 -0.10782019006398258 1105 68-69 -0.07775757476551348 1105 70-71 -0.2605057287477486 1105 72-73 -0.21191227724282413 1105 74-75 -0.2305566989674972 1105 76-77 -0.07287233801056558 1105 78-79 -0.18631423172947592 1105 80-81 -0.2624771789472895 1105 82-83 -0.22298904379402273 1105 84-85 -0.29237337621647796 1105 86-87 -0.5238452561428062 1105 88-89 -0.7440552200619521 1105 90-91 -0.8703941050999404 1105 92-93 -0.7456468193472503 1105 94-95 -0.8576452018179914 1105 96-97 -1.1510720138357975 1105 98-99 -1.13912917572598 1105 100-101 -1.7388290288345836 1106 1 0.16461873511635616 1106 2 0.22633439954296364 1106 3 0.22307016863429396 1106 4 0.18771402147462624 1106 5 0.19439142364321782 1106 6 0.13995844553306114 1106 7 0.16939669626699327 1106 8 0.2386813272536088 1106 9 0.23818687456449084 1106 10-11 0.21524814401661274 1106 12-13 0.2047672479063749 1106 14-15 0.28686582488001733 1106 16-17 0.35515142540391764 1106 18-19 0.2802419634671338 1106 20-21 0.31355925455145695 1106 22-23 0.3328599654531814 1106 24-25 0.41854802402146873 1106 26-27 0.3771742145878605 1106 28-29 0.37037861991288423 1106 30-31 0.41236108628077517 1106 32-33 0.42690019137251056 1106 34-35 0.5151070347694073 1106 36-37 0.48299688436026145 1106 38-39 0.46583551802705614 1106 40-41 0.43691125474363446 1106 42-43 0.400229473114031 1106 44-45 0.4854468475051945 1106 46-47 0.4695473830221175 1106 48-49 0.4412476438672783 1106 50-51 0.32883318434863185 1106 52-53 0.22368449261031031 1106 54-55 0.1372922729282493 1106 56-57 0.24799342860061557 1106 58-59 0.3547890385111643 1106 60-61 0.1939538940291854 1106 62-63 0.4012244379180814 1106 64-65 0.39798823863083754 1106 66-67 0.028626979513624917 1106 68-69 -0.060578830804161043 1106 70-71 -0.28923259608331264 1106 72-73 -0.2555240696372252 1106 74-75 -0.22222671011576622 1106 76-77 -0.27006792824963455 1106 78-79 -0.3039678920787878 1106 80-81 -0.2624801518198261 1106 82-83 -0.27088053394638223 1106 84-85 -0.20205230598236668 1106 86-87 -0.3275477211496174 1106 88-89 -0.5571752497906779 1106 90-91 -0.6977507259348243 1106 92-93 -0.7171052610665605 1106 94-95 -0.8959895595536551 1106 96-97 -0.8866213759068948 1106 98-99 -1.0795243200341638 1106 100-101 -1.5262146351767107 1107 1 0.06256811207622803 1107 2 0.0860644246403055 1107 3 0.09353969577548327 1107 4 0.031504031231648355 1107 5 -0.00831564772951765 1107 6 0.025828148044446664 1107 7 0.02418743276687252 1107 8 -0.02298289558002864 1107 9 0.026143445729459813 1107 10-11 0.01137828407478736 1107 12-13 0.003000457308999671 1107 14-15 0.057810480757211735 1107 16-17 0.04214986757477135 1107 18-19 0.03922117349581811 1107 20-21 0.04368292897797943 1107 22-23 0.08692139627623163 1107 24-25 0.13928179632944904 1107 26-27 0.0804140781725522 1107 28-29 0.013442619048852578 1107 30-31 -0.006599396847626338 1107 32-33 0.08921326560462717 1107 34-35 0.0745125884028468 1107 36-37 0.0028068511717336264 1107 38-39 -0.009660891277455619 1107 40-41 -0.12115055684141396 1107 42-43 -0.09716798200456367 1107 44-45 -0.07888572979590691 1107 46-47 -0.057551979384591334 1107 48-49 -0.1509569864183149 1107 50-51 -0.14311939247651395 1107 52-53 -0.04456050576961701 1107 54-55 -0.019857174326148197 1107 56-57 0.05879755034926859 1107 58-59 0.03796503293153819 1107 60-61 -0.008335986671490048 1107 62-63 -0.14561862406741 1107 64-65 -0.2310108149559298 1107 66-67 -0.19824420028109557 1107 68-69 -0.028268795345702813 1107 70-71 -0.16728624325240204 1107 72-73 -0.28487633489653774 1107 74-75 -0.32179807827784046 1107 76-77 -0.40394695943001935 1107 78-79 -0.21665468055268278 1107 80-81 -0.11500919865172321 1107 82-83 -0.2105216108438306 1107 84-85 -0.4958282202230464 1107 86-87 -0.594503895077306 1107 88-89 -0.846946298332341 1107 90-91 -0.9375351377582177 1107 92-93 -0.9808590069042609 1107 94-95 -1.1169570062332426 1107 96-97 -1.138835195736906 1107 98-99 -1.1826011698876258 1107 100-101 -1.5218354145306243 1108 1 -2.7054398719883466E-4 1108 2 0.02162994943638097 1108 3 -0.02509715501106058 1108 4 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10 2355.0 11 4121.0 12 6315.0 13 9895.0 14 12993.0 15 17813.0 16 23043.0 17 32751.0 18 33536.0 19 25655.0 20 23551.0 21 24966.0 22 26863.0 23 29535.0 24 33421.0 25 38743.0 26 44452.0 27 52584.0 28 63479.0 29 76743.0 30 92858.0 31 114519.0 32 142847.0 33 186744.0 34 252494.0 35 363646.0 36 532660.0 37 706489.0 38 427721.0 39 22793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12313580880808 19.30575652904521 15.311777954115808 23.2593297080309 2 15.346694394749402 20.0798407735855 40.48370099884116 24.089763832823934 3 18.769201595787354 24.081860682220974 30.260533436412835 26.888404285578837 4 12.813339027489473 17.20300250052799 37.384877114743084 32.59878135723945 5 13.47046708906547 37.28952987713095 34.75822919219146 14.481773841612117 6 31.70622705664631 36.8463550843522 17.271313252477825 14.176104606523673 7 28.2120559135632 32.885404849424326 20.7644110677794 18.138128169233074 8 25.826141011418358 35.42989261909811 19.719743751380147 19.024222618103387 9 25.880836398949032 16.952886414161643 20.61826499229743 36.54801219459189 10-11 24.691598234228696 26.69219507029743 27.459622406381186 21.156584289092688 12-13 25.497426230569836 24.054621787455595 27.911454513737567 22.53649746823701 14-15 22.26641933655516 25.581499482071145 26.383596401131015 25.768484780242684 16-17 21.778521654469277 28.83624867360222 27.156473003475075 22.22875666845343 18-19 21.653918508831627 27.27085768493531 29.604060818937267 21.471162987295795 20-21 22.222706782840643 26.411775464786817 29.9490063962974 21.41651135607514 22-23 22.04769612817915 26.28371533078166 29.71237227168468 21.95621626935451 24-25 21.93102367910468 26.47536041125346 29.73065022072901 21.86296568891285 26-27 21.538674527619953 26.907839445271197 29.66445892219608 21.88902710491277 28-29 21.621187969538763 27.058969050991998 29.667323252714834 21.652519726754406 30-31 21.808470759697393 26.59083821646537 29.59178017693921 22.008910846898022 32-33 21.591434498116584 26.690415647023098 29.50811781649012 22.2100320383702 34-35 22.17066299314453 26.80841175888835 29.26258535789855 21.75833989006857 36-37 22.255817589698175 26.448286082008092 29.24733930523808 22.048557023055658 38-39 21.752120707716465 26.766512552716858 29.290606166950067 22.190760572616608 40-41 22.23544824600657 26.52543490297275 29.243983236902153 21.99513361411853 42-43 22.017943564266897 26.508312499817677 29.131424720753095 22.342319215162327 44-45 21.962488007124694 26.81316668207861 29.161640768279124 22.06270454251757 46-47 22.190338420447873 27.042816766154047 28.54901281273765 22.21783200066043 48-49 22.123486184498688 26.90420491057384 28.625051286423204 22.347257618504273 50-51 22.282478768926662 27.193189603479993 28.36474023504818 22.159591392545163 52-53 22.470437032618925 27.101337719806857 28.169731086359644 22.25849416121457 54-55 22.162086785237722 27.181737770208336 28.352745099068855 22.303430345485086 56-57 22.185968232674657 27.327447534518807 28.272446907971677 22.214137324834855 58-59 22.287460278232544 26.967721021674055 28.169199629910274 22.575619070183123 60-61 22.11525698834612 27.306614406190043 28.288015281405222 22.290113324058616 62-63 21.986667417480284 27.422023094521116 28.277279553293127 22.314029934705474 64-65 21.79695496342945 27.27890485588274 28.23697797830859 22.687162202379216 66-67 21.818157152278495 27.21365773876339 28.21706403370614 22.75112107525197 68-69 22.009674020834762 27.070173490708456 28.31487241004449 22.605280078412296 70-71 22.019043217697227 27.209998367624166 27.97518730382132 22.79577111085728 72-73 22.121248675029108 27.252195254507456 27.73116120033064 22.895394870132797 74-75 21.88476425311993 27.55073892315482 27.788345018404698 22.77615180532055 76-77 22.022305863130406 27.233547784731645 27.936350241440177 22.80779611069777 78-79 22.015395712169862 27.390137337082177 28.00799080762421 22.58647614312375 80-81 22.038659396051962 27.645981435251084 27.694162726439668 22.621196442257283 82-83 22.171155437080614 27.53379526888121 27.670946063261532 22.624103230776637 84-85 22.127702719459872 27.42717008988033 27.66817366846252 22.776953522197278 86-87 22.160365543354434 27.425439333669487 27.56563451267111 22.848560610304965 88-89 22.10432810223259 27.64589962658892 27.390394016086027 22.859378255092462 90-91 22.1523028081888 27.539960666682227 27.454772520366955 22.852964004762015 92-93 22.141744630093882 27.20488858699458 27.56605299645663 23.087313786454907 94-95 21.92672381096867 27.234550324135398 27.60799164425046 23.23073422064547 96-97 22.038708024780323 27.12696619575803 27.570751537975436 23.26357424148621 98-99 21.971997196364608 27.238907807511904 27.271057715941556 23.51803728018193 100-101 22.28568669558573 26.75920515256903 27.276705525264994 23.67840262658024 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1012.0 1 692.5 2 506.0 3 654.0 4 534.0 5 492.5 6 1026.5 7 2418.5 8 4024.5 9 4631.5 10 3891.5 11 3117.0 12 3265.0 13 3573.0 14 3870.5 15 4259.5 16 4451.0 17 4677.5 18 5091.0 19 5616.0 20 6323.0 21 7445.0 22 8940.5 23 10954.0 24 13569.0 25 17065.0 26 21834.5 27 27304.5 28 32124.0 29 37478.5 30 44010.5 31 50844.0 32 59070.5 33 65171.5 34 69059.0 35 73361.0 36 79419.0 37 86070.5 38 90443.0 39 93832.0 40 97735.5 41 105042.0 42 110881.5 43 117102.0 44 125080.5 45 130025.5 46 130384.0 47 131152.5 48 131853.0 49 127236.5 50 123942.5 51 119595.0 52 114428.5 53 111772.5 54 110446.0 55 103750.5 56 93666.5 57 85233.5 58 77803.0 59 69260.0 60 56311.0 61 45939.0 62 38517.0 63 29343.5 64 22308.0 65 17772.5 66 12926.5 67 10080.0 68 7792.0 69 5287.0 70 3882.5 71 2764.0 72 2004.5 73 1521.0 74 1223.5 75 1071.0 76 676.0 77 310.5 78 153.5 79 90.5 80 46.0 81 23.0 82 12.5 83 5.0 84 2.5 85 1.0 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009247166851853094 2 0.014731291041595622 3 0.00880960375160768 4 2.91708733496943E-5 5 0.0010793223139386892 6 0.0020127902611289067 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.042970738405235E-4 14-15 0.0 16-17 0.00261079316479764 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003442163055263928 26-27 0.01961741232766942 28-29 0.007146863970675104 30-31 0.0035005048019633167 32-33 0.0 34-35 0.008867945498307068 36-37 7.292718337423576E-4 38-39 0.0027420620948712645 40-41 0.0020711320078282956 42-43 0.00312128344841729 44-45 0.0 46-47 1.166834933987772E-4 48-49 7.438572704172047E-4 50-51 0.004127678578981744 52-53 0.007774037747693531 54-55 0.007555256197570825 56-57 0.01637944538585335 58-59 0.023336698679755442 60-61 0.0035005048019633167 62-63 0.019952877371190902 64-65 0.021265566671927146 66-67 0.013199820190736672 68-69 0.03102322380739989 70-71 0.016262761892454573 72-73 0.005002804779472574 74-75 0.016204420145755186 76-77 0.02120722492522776 78-79 0.025932906407878237 80-81 0.011610007593178333 82-83 0.03477168103283561 84-85 0.021644788025473174 86-87 0.031183663610823212 88-89 0.0220240093790192 90-91 0.02791652579565745 92-93 0.02418265400689658 94-95 0.01449792405479807 96-97 0.015139683268491343 98-99 0.01121620080295746 100-101 0.010209805672393006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3428077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.76394670125958 #Duplication Level Percentage of deduplicated Percentage of total 1 67.8982694730023 30.39394515797235 2 15.956215077240433 14.285263225428505 3 6.371708834512364 8.556685039921687 4 3.2317607228704186 5.786654589991821 5 1.8211603452117187 4.076116231375243 6 1.1122909575899993 2.9874319885111027 7 0.7591121805490136 2.378660003326109 8 0.49857003084316104 1.7854369830006882 9 0.3871203042652332 1.559612940039386 >10 1.7656289572889454 13.82116274991879 >50 0.11593528032654303 3.579978763605749 >100 0.07381340518543456 6.220624746958082 >500 0.005218251857145682 1.5920879612502628 >1k 0.0030004948132031314 2.0154398157708515 >5k 6.522814811311156E-5 0.2180850414196666 >10k+ 1.3045629622622312E-4 0.7428147615097495 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13943 0.4067294871147877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11497 0.3353775309014354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7469 0.21787725304886676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9170873349694305E-5 2 0.0 0.0 0.0 0.0 2.9170873349694305E-5 3 0.0 0.0 0.0 0.0 2.9170873349694305E-5 4 0.0 0.0 0.0 0.0 2.9170873349694305E-5 5 0.0 0.0 0.0 0.0 5.834174669938861E-5 6 0.0 0.0 0.0 0.0 5.834174669938861E-5 7 0.0 0.0 0.0 0.0 5.834174669938861E-5 8 0.0 0.0 0.0 0.0 5.834174669938861E-5 9 0.0 0.0 0.0 0.0 5.834174669938861E-5 10-11 0.0 0.0 0.0 0.0 5.834174669938861E-5 12-13 0.0 0.0 0.0 1.4585436674847152E-5 7.292718337423576E-5 14-15 0.0 0.0 0.0 5.834174669938861E-5 1.0209805672393006E-4 16-17 0.0 0.0 0.0 8.751262004908292E-5 1.4585436674847152E-4 18-19 0.0 0.0 0.0 1.1668349339877722E-4 1.6043980342331866E-4 20-21 0.0 0.0 0.0 1.4585436674847152E-4 2.3336698679755444E-4 22-23 0.0 0.0 0.0 3.062941701717902E-4 2.3336698679755444E-4 24-25 0.0 0.0 0.0 4.375631002454146E-4 2.3336698679755444E-4 26-27 0.0 0.0 0.0 9.918096938896063E-4 2.3336698679755444E-4 28-29 0.0 0.0 0.0 0.003967238775558425 2.3336698679755444E-4 30-31 0.0 0.0 0.0 0.012455962920319467 2.3336698679755444E-4 32-33 0.0 0.0 0.0 0.03240884029151037 2.3336698679755444E-4 34-35 0.0 0.0 0.0 0.061652640824578914 2.3336698679755444E-4 36-37 0.0 0.0 0.0 0.10925950613128002 2.3336698679755444E-4 38-39 0.0 0.0 0.0 0.17871535557690216 2.3336698679755444E-4 40-41 0.0 0.0 0.0 0.25002355548022986 2.3336698679755444E-4 42-43 0.0 0.0 0.0 0.32362166894150857 2.3336698679755444E-4 44-45 0.0 0.0 0.0 0.4164142170668862 2.3336698679755444E-4 46-47 0.0 0.0 0.0 0.5225961960597735 2.479524234724016E-4 48-49 0.0 0.0 0.0 0.6319432148111026 3.062941701717902E-4 50-51 0.0 0.0 0.0 0.7468035286255239 3.5005048019633167E-4 52-53 0.0 0.0 0.0 0.85596093670008 3.7922135354602596E-4 54-55 0.0 0.0 0.0 0.9783035795286978 3.7922135354602596E-4 56-57 0.0 0.0 0.0 1.1170694240531938 3.7922135354602596E-4 58-59 0.0 0.0 0.0 1.2643969199058247 3.7922135354602596E-4 60-61 0.0 0.0 0.0 1.4195276243794992 3.7922135354602596E-4 62-63 0.0 0.0 0.0 1.5718579250116027 4.3756310024541453E-4 64-65 0.0 0.0 0.0 1.7345147148094981 4.3756310024541453E-4 66-67 0.0 0.0 0.0 1.9154178858876274 4.521485369202617E-4 68-69 0.0 0.0 0.0 2.110527272287058 4.667339735951089E-4 70-71 0.0 0.0 0.0 2.320528389531507 4.8131941026995605E-4 72-73 0.0 0.0 0.0 2.5310108261862263 4.959048469448032E-4 74-75 0.0 0.0 0.0 2.7496756928155346 5.104902836196503E-4 76-77 0.0 0.0 0.0 2.983669853390108 5.250757202944975E-4 78-79 0.0 0.0 0.0 3.2384482612263374 5.396611569693446E-4 80-81 0.0 0.0 0.0 3.503407303861611 5.834174669938861E-4 82-83 0.0 0.0 0.0 3.777948978392259 6.125883403435804E-4 84-85 0.0 0.0 0.0 4.058645707199693 6.125883403435804E-4 86-87 0.0 0.0 0.0 4.349245947509347 6.125883403435804E-4 88-89 0.0 0.0 0.0 4.658369692396057 6.125883403435804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 46.538006 1 GTATCAA 12830 0.0 40.437336 1 ATCAACG 15810 0.0 32.536366 3 TATCAAC 15990 0.0 32.263924 2 TCAACGC 15980 0.0 32.185074 4 CAACGCA 16215 0.0 31.71862 5 AACGCAG 16820 0.0 30.662432 6 ACGCAGA 18875 0.0 27.247812 7 CGCAGAG 18880 0.0 27.089676 8 GCAGAGT 20730 0.0 24.259775 9 GAGTACT 12915 0.0 23.828465 12-13 GTACATG 10425 0.0 22.467636 1 GTACTTT 13680 0.0 22.148792 14-15 TACATGG 10255 0.0 21.910955 2 ACATGGG 10055 0.0 21.115334 3 AGAGTAC 18510 0.0 21.026066 10-11 CAGAGTA 20010 0.0 20.945131 10-11 AGTACTT 13610 0.0 20.692455 12-13 GTGTAGC 1700 0.0 20.680895 1 GGACCGA 1605 0.0 20.41629 6 >>END_MODULE