FastQCFastQC Report
Thu 26 May 2016
SRR938187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470434
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109110.44166328669375504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88450.3580342563290499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54800.2218233719257426No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25910.10488035705467137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.045.6834951
GTATCAA97250.039.7848241
TATCAAC122150.031.479512
ATCAACG122650.031.2349193
TCAACGC123850.030.9322764
CAACGCA126300.030.3322455
AACGCAG130850.029.343626
ACGCAGA147050.025.9117937
GTAAGGT12400.025.6818334
CGCAGAG148450.025.6034168
TAAGGTG13450.025.4438675
GCAGAGT158700.023.4992759
GTACATG88100.023.2309841
TACATGG86300.022.9116022
GAGTACT94000.022.79069912-13
AGAGTAC133350.021.60681210-11
GTACTTT99300.021.33639114-15
AGTACTT97750.020.87158412-13
ACATGGG89800.020.6424483
CAGAGTA150200.020.44803410-11
Produced by FastQC (version 0.11.5)