FastQCFastQC Report
Thu 26 May 2016
SRR938187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470434
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106110.4295196714423457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.3694492546653746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.23085012592929016No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25950.10504227192469015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63800.044.210151
GTATCAA102500.037.727211
ATCAACG124750.030.724563
TCAACGC125100.030.6386014
TATCAAC126700.030.4467562
CAACGCA128100.029.80975
AACGCAG132250.028.8745376
TAAGGTG12600.026.4191425
ACGCAGA150200.025.3605757
CGCAGAG152100.024.9813218
GAGTACT95150.022.86138512-13
GTACATG87850.022.6319771
TACATGG84600.022.6022932
GCAGAGT165750.022.4651019
GTAAGGT13400.022.3576894
GTACTTT97350.021.88126214-15
AGAGTAC134400.021.04400310-11
AGTACTT97550.020.52183512-13
ACATGGG88100.020.5115683
CAGAGTA155300.020.4444710-11
Produced by FastQC (version 0.11.5)