##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938187_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2470434 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46484423384717 33.0 31.0 34.0 31.0 34.0 2 32.692397773022876 34.0 31.0 34.0 31.0 34.0 3 32.755546191478906 34.0 31.0 34.0 31.0 34.0 4 36.22073085134029 37.0 35.0 37.0 35.0 37.0 5 36.14660743820721 37.0 35.0 37.0 35.0 37.0 6 36.19767619778549 37.0 37.0 37.0 35.0 37.0 7 36.16847404140325 37.0 37.0 37.0 35.0 37.0 8 36.163448203837866 37.0 37.0 37.0 35.0 37.0 9 37.85118930519901 39.0 38.0 39.0 35.0 39.0 10-11 37.824568071844865 39.0 38.0 39.0 35.0 39.0 12-13 37.74027579769385 39.0 38.0 39.0 35.0 39.0 14-15 38.97984848006463 40.0 38.0 41.0 35.5 41.0 16-17 38.887511870383904 40.0 38.0 41.0 35.0 41.0 18-19 39.133361182690976 40.0 38.5 41.0 36.0 41.0 20-21 39.102401035607514 40.0 39.0 41.0 36.0 41.0 22-23 38.995892624534804 40.0 38.5 41.0 35.0 41.0 24-25 38.88171612761158 40.0 38.0 41.0 35.0 41.0 26-27 38.7173014943933 40.0 38.0 41.0 35.0 41.0 28-29 38.590756320549346 40.0 38.0 41.0 34.0 41.0 30-31 38.34732257570937 40.0 38.0 41.0 34.0 41.0 32-33 38.1168760225936 40.0 38.0 41.0 34.0 41.0 34-35 38.02789489620042 40.0 37.5 41.0 33.0 41.0 36-37 37.97866953741731 40.0 37.5 41.0 33.0 41.0 38-39 37.78529845363204 40.0 37.0 41.0 33.0 41.0 40-41 37.56283005334285 40.0 37.0 41.0 33.0 41.0 42-43 37.2974752614318 40.0 36.5 41.0 32.0 41.0 44-45 37.0145219422984 40.0 36.0 41.0 31.0 41.0 46-47 36.62902651922698 39.0 36.0 41.0 30.5 41.0 48-49 36.194445996128614 39.0 35.0 40.5 30.0 41.0 50-51 35.791396774817706 39.0 35.0 40.0 28.5 41.0 52-53 35.23145811626621 38.0 34.5 40.0 27.0 41.0 54-55 34.83035794520315 38.0 34.0 40.0 26.0 41.0 56-57 34.41320796264948 38.0 34.0 40.0 25.5 41.0 58-59 33.87310488764322 37.0 33.0 40.0 23.5 41.0 60-61 33.36824622718113 36.5 32.5 39.0 22.5 41.0 62-63 32.78323383664571 36.0 32.0 39.0 21.0 40.0 64-65 32.18135214298378 35.0 31.0 38.5 18.5 40.0 66-67 31.589409391224375 35.0 31.0 38.0 15.0 40.0 68-69 30.939879187219734 35.0 30.0 37.5 9.0 39.5 70-71 30.301885012916756 34.0 29.0 37.0 7.0 39.0 72-73 29.61745547543468 34.0 28.5 36.0 3.5 39.0 74-75 28.950183044760557 34.0 27.5 35.5 2.0 38.5 76-77 27.676790798701767 31.5 26.0 34.5 2.0 36.0 78-79 28.330635629205233 33.0 27.0 35.0 2.0 37.0 80-81 28.05930779773918 33.0 27.0 35.0 2.0 36.5 82-83 27.50793119751428 33.0 26.0 35.0 2.0 36.0 84-85 26.854628377038203 33.0 25.0 35.0 2.0 35.5 86-87 26.137813032042146 32.0 23.5 35.0 2.0 35.0 88-89 25.31815644538571 31.5 20.0 34.0 2.0 35.0 90-91 24.646019889622632 31.0 17.5 34.0 2.0 35.0 92-93 23.694810506979746 31.0 6.0 34.0 2.0 35.0 94-95 22.767134843513325 30.0 2.0 34.0 2.0 35.0 96-97 21.695281274464325 29.5 2.0 34.0 2.0 35.0 98-99 20.259633934766118 29.0 2.0 34.0 2.0 35.0 100-101 16.45199446736889 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2116325031554851 1101 2 0.16546905347773588 1101 3 0.16919337891381048 1101 4 0.09638620018828448 1101 5 0.06292651988608355 1101 6 0.08481180012524447 1101 7 0.12458130144532475 1101 8 0.17663184064592485 1101 9 0.0341098172240919 1101 10-11 0.17341568456642165 1101 12-13 0.2732577036003647 1101 14-15 0.3831628920787864 1101 16-17 0.4135015161671802 1101 18-19 0.3023235195365501 1101 20-21 0.20256644980088367 1101 22-23 0.3556286146286425 1101 24-25 0.38982973902737683 1101 26-27 0.37859728236048795 1101 28-29 0.3810515276846971 1101 30-31 0.5693846098120758 1101 32-33 0.49118083274039037 1101 34-35 0.3611767149099663 1101 36-37 0.545329853244084 1101 38-39 0.6554256346887328 1101 40-41 0.6746873559277446 1101 42-43 0.780348833061133 1101 44-45 0.7121405109200722 1101 46-47 0.8053828515911476 1101 48-49 0.9257753101469035 1101 50-51 0.9229355586795762 1101 52-53 1.0969526080004712 1101 54-55 1.2396477849297014 1101 56-57 1.2326022890100035 1101 58-59 1.418169031772031 1101 60-61 1.32934344582101 1101 62-63 1.387515197574217 1101 64-65 1.5555999447937765 1101 66-67 1.827369441566006 1101 68-69 2.0285620232716433 1101 70-71 1.968370950226511 1101 72-73 2.085121594661082 1101 74-75 2.1617777794255133 1101 76-77 2.199638287579038 1101 78-79 2.4423279103909046 1101 80-81 2.480686212934444 1101 82-83 2.3949562681161325 1101 84-85 2.620058094307403 1101 86-87 2.9073528159592747 1101 88-89 3.242507671183514 1101 90-91 3.4611208055368934 1101 92-93 3.7132096809369166 1101 94-95 3.914644796141719 1101 96-97 4.112697468842935 1101 98-99 4.456629212783941 1101 100-101 4.361462525469257 1102 1 0.14261351677509282 1102 2 0.13524765321397325 1102 3 0.09074008870555161 1102 4 0.1276758782608738 1102 5 -0.0570069114663454 1102 6 0.016030648091458488 1102 7 0.08015247217709032 1102 8 0.06572903977715328 1102 9 0.1829279490654656 1102 10-11 0.13133242631494113 1102 12-13 0.1849676846943069 1102 14-15 0.30536016099972585 1102 16-17 0.27421350855315296 1102 18-19 0.2170238045632189 1102 20-21 0.1923132508977048 1102 22-23 0.2990792851217421 1102 24-25 0.29447460428163197 1102 26-27 0.22505710874766294 1102 28-29 0.07586397147733948 1102 30-31 0.23325726044360806 1102 32-33 0.17093272345061905 1102 34-35 0.17143588568168155 1102 36-37 0.2735302441829859 1102 38-39 0.4119476642251385 1102 40-41 0.46131634225404383 1102 42-43 0.48496200787707977 1102 44-45 0.4623128003680108 1102 46-47 0.5771138986074433 1102 48-49 0.6122751001951485 1102 50-51 0.7775722672353353 1102 52-53 0.753902156739457 1102 54-55 1.01606269121298 1102 56-57 1.1238031672700401 1102 58-59 1.158710070577527 1102 60-61 0.993810058081948 1102 62-63 1.287839093413183 1102 64-65 1.4138962435253717 1102 66-67 1.491715888179371 1102 68-69 1.6650371143374336 1102 70-71 1.620632894275726 1102 72-73 1.9547252207297277 1102 74-75 2.2026878880302903 1102 76-77 2.234112511371375 1102 78-79 2.3545920059774232 1102 80-81 2.4539230863720327 1102 82-83 2.539113741503865 1102 84-85 2.604486707173429 1102 86-87 2.7000614557756997 1102 88-89 2.90205082115898 1102 90-91 3.07873093033022 1102 92-93 3.2611014656849733 1102 94-95 3.5657317178328363 1102 96-97 3.75082866101517 1102 98-99 3.9968288354950197 1102 100-101 3.7088562106032192 1103 1 0.22070002540038303 1103 2 0.18613051962518057 1103 3 0.16043589295063754 1103 4 0.12854394465355767 1103 5 0.019990235251171384 1103 6 0.04577465811117065 1103 7 0.14079046372396675 1103 8 0.12863938790983553 1103 9 0.22412797886686775 1103 10-11 0.2378772562758158 1103 12-13 0.2749456558231884 1103 14-15 0.37154463567961216 1103 16-17 0.4553680509506748 1103 18-19 0.34567311661405853 1103 20-21 0.3022055027356245 1103 22-23 0.35336796804090653 1103 24-25 0.37246687605427553 1103 26-27 0.40673307492710364 1103 28-29 0.4679950936006563 1103 30-31 0.455320765611539 1103 32-33 0.4850856690570282 1103 34-35 0.5780851212154303 1103 36-37 0.6182359654472904 1103 38-39 0.6414577936799901 1103 40-41 0.6545225296685899 1103 42-43 0.7406074598414492 1103 44-45 0.863216910343219 1103 46-47 0.805070529519142 1103 48-49 0.8661153526210441 1103 50-51 1.0126825424754102 1103 52-53 1.2184897396245873 1103 54-55 1.138240569313183 1103 56-57 1.2291299657743266 1103 58-59 1.3315232419899345 1103 60-61 1.4105856273792554 1103 62-63 1.461516203321942 1103 64-65 1.6639781973694454 1103 66-67 1.7655930500392216 1103 68-69 1.8440488758215459 1103 70-71 1.8258889144601333 1103 72-73 2.058889865398399 1103 74-75 2.1369983581876113 1103 76-77 2.107750557285666 1103 78-79 2.3332460500069736 1103 80-81 2.5144367376272143 1103 82-83 2.7078813731585853 1103 84-85 2.869341395154809 1103 86-87 3.095356157369064 1103 88-89 3.1596213830160096 1103 90-91 3.5983897848691946 1103 92-93 3.636717579381333 1103 94-95 3.6823147862089236 1103 96-97 3.98585668036997 1103 98-99 4.289522358322287 1103 100-101 3.9131197234228488 1104 1 0.23441578036010924 1104 2 0.19155422063591487 1104 3 0.22490764717029066 1104 4 0.13023333167138418 1104 5 -0.01032450535153373 1104 6 0.07994864761663933 1104 7 0.12305867131809833 1104 8 0.09705889454092187 1104 9 0.11311825683273469 1104 10-11 0.1897864715129174 1104 12-13 0.27311578855938023 1104 14-15 0.3733923371633807 1104 16-17 0.2966655238321678 1104 18-19 0.2980684335577095 1104 20-21 0.3452843948395454 1104 22-23 0.3647118671163909 1104 24-25 0.43085954552227435 1104 26-27 0.4499313704715959 1104 28-29 0.38354834543700633 1104 30-31 0.435582575775328 1104 32-33 0.41920615590262145 1104 34-35 0.48522386381709026 1104 36-37 0.6657783717890311 1104 38-39 0.694329770684341 1104 40-41 0.6317934362382545 1104 42-43 0.802639645363115 1104 44-45 0.8828437078323148 1104 46-47 0.9526374459920461 1104 48-49 0.864424058200008 1104 50-51 1.0934227293627714 1104 52-53 0.9794456728883603 1104 54-55 1.0795997466883307 1104 56-57 1.1425138325765545 1104 58-59 1.4335999272283502 1104 60-61 1.4512438340769123 1104 62-63 1.567180427876707 1104 64-65 1.5608070441725417 1104 66-67 1.7943548783233325 1104 68-69 1.9172464274787977 1104 70-71 2.0541214383551214 1104 72-73 2.2414978768733356 1104 74-75 2.2796099365065388 1104 76-77 2.126199914776894 1104 78-79 2.0686025341287966 1104 80-81 2.2940953315277213 1104 82-83 2.5160916948802843 1104 84-85 2.820014613115678 1104 86-87 2.849131480530854 1104 88-89 2.925645286592964 1104 90-91 3.357628769138838 1104 92-93 3.61547091972119 1104 94-95 3.9395513785949525 1104 96-97 4.1598670719026245 1104 98-99 4.544980392590691 1104 100-101 3.955197705695447 1105 1 0.19488471859683898 1105 2 0.2076542431964583 1105 3 0.19690635952517255 1105 4 0.11256120499707123 1105 5 0.04378619467620126 1105 6 0.04272589482042832 1105 7 0.12923562264886357 1105 8 0.10707523978675937 1105 9 0.27285366537916644 1105 10-11 0.18940445823876217 1105 12-13 0.2289702489923826 1105 14-15 0.3836184151871862 1105 16-17 0.33640658588971206 1105 18-19 0.25829544788856396 1105 20-21 0.22228334350050005 1105 22-23 0.26519732002594054 1105 24-25 0.3238662236208043 1105 26-27 0.3213827444798909 1105 28-29 0.43400227600939445 1105 30-31 0.41031601807696916 1105 32-33 0.5105761692873543 1105 34-35 0.5169693095750318 1105 36-37 0.4966069500201584 1105 38-39 0.4983346680160423 1105 40-41 0.6328422784723102 1105 42-43 0.6195697709976926 1105 44-45 0.6916005863451389 1105 46-47 0.5900753710642945 1105 48-49 0.6180157766087859 1105 50-51 0.7212835709512078 1105 52-53 0.8969194808134446 1105 54-55 1.0506753494694294 1105 56-57 1.059281040785379 1105 58-59 1.1911339441549913 1105 60-61 1.1850335502153655 1105 62-63 1.252394356201961 1105 64-65 1.426387594667581 1105 66-67 1.4781420530569562 1105 68-69 1.7929202787415157 1105 70-71 1.8116749839715531 1105 72-73 1.988180273498351 1105 74-75 1.936992717552478 1105 76-77 1.846569408288289 1105 78-79 1.9744334977135871 1105 80-81 2.0608944187621105 1105 82-83 2.1360511750782827 1105 84-85 2.5217334945051952 1105 86-87 2.5879908980408644 1105 88-89 2.47771983090124 1105 90-91 2.911100016041221 1105 92-93 2.981867573270641 1105 94-95 3.331263277809075 1105 96-97 3.3512161298439764 1105 98-99 3.4241713698009804 1105 100-101 3.553119817433437 1106 1 0.1399757904775214 1106 2 0.13564039427826913 1106 3 0.10842957407214726 1106 4 0.1406778029360254 1106 5 0.13338224745417193 1106 6 0.10540766844212612 1106 7 0.12820536405921246 1106 8 0.12286132446244835 1106 9 0.20555324950863252 1106 10-11 0.21125513907673366 1106 12-13 0.17445721700968164 1106 14-15 0.10883819930596417 1106 16-17 0.0627339855993796 1106 18-19 0.004515423866713775 1106 20-21 0.03329580910379093 1106 22-23 -0.011328202966076617 1106 24-25 0.02621707047408961 1106 26-27 -0.09520906198568468 1106 28-29 0.05651570397582617 1106 30-31 0.1532277776552604 1106 32-33 0.277094780085271 1106 34-35 0.18096562657579085 1106 36-37 0.31422726293492076 1106 38-39 0.34411923709023995 1106 40-41 0.342297879671122 1106 42-43 0.2775282995851711 1106 44-45 0.40251107737374525 1106 46-47 0.6124928693406346 1106 48-49 0.8258960283212815 1106 50-51 0.9627821416426272 1106 52-53 1.038347265030339 1106 54-55 1.0941107362237688 1106 56-57 1.1316558306380244 1106 58-59 1.341114276378356 1106 60-61 1.2379220851718813 1106 62-63 1.1875176762277846 1106 64-65 1.288925867985725 1106 66-67 1.4795760048082656 1106 68-69 1.4394588814620626 1106 70-71 1.342832859711507 1106 72-73 1.6357627138971012 1106 74-75 1.8901164773655594 1106 76-77 1.8075672873222643 1106 78-79 1.9656317077268959 1106 80-81 1.7704634710556206 1106 82-83 2.1992895161166537 1106 84-85 2.3738011516568633 1106 86-87 2.4618935543734075 1106 88-89 2.6497831777873806 1106 90-91 2.7579780083685996 1106 92-93 2.9413595751874837 1106 94-95 3.1775467688788517 1106 96-97 3.4370902858598207 1106 98-99 3.73673526817333 1106 100-101 3.6690550092621557 1107 1 0.20424153623149977 1107 2 0.14230799736341027 1107 3 0.1332158237126677 1107 4 0.04913303121605139 1107 5 0.041187879205622835 1107 6 0.10758326511992067 1107 7 0.10731266535605499 1107 8 0.14803165746430125 1107 9 0.22418173038450107 1107 10-11 0.1696951476376114 1107 12-13 0.1807750642933783 1107 14-15 0.36795155346234765 1107 16-17 0.2950246426533951 1107 18-19 0.18624517559789666 1107 20-21 0.1795996066076171 1107 22-23 0.16428441262234372 1107 24-25 0.21522338919707806 1107 26-27 0.28741353906957556 1107 28-29 0.3296264709550982 1107 30-31 0.372060980392078 1107 32-33 0.40531522186694957 1107 34-35 0.42201138766507995 1107 36-37 0.5209188427697953 1107 38-39 0.4453936827789562 1107 40-41 0.5797318034968626 1107 42-43 0.7687993790713392 1107 44-45 0.8249937550915476 1107 46-47 0.8588382101975398 1107 48-49 0.8984777130268498 1107 50-51 0.9677162029537598 1107 52-53 1.006251872244242 1107 54-55 1.244982813871303 1107 56-57 1.1820908098610943 1107 58-59 1.2413960425943529 1107 60-61 1.2923031575158177 1107 62-63 1.297854860436125 1107 64-65 1.4164889194816155 1107 66-67 1.4209035496276243 1107 68-69 1.65326011928585 1107 70-71 1.848298034927435 1107 72-73 1.7666360980936027 1107 74-75 1.6400954604921623 1107 76-77 1.7633583817023855 1107 78-79 1.8808373806013599 1107 80-81 1.9738763310702794 1107 82-83 2.2455874989521725 1107 84-85 2.2975611298553567 1107 86-87 2.5168597715008296 1107 88-89 2.605640387748398 1107 90-91 2.75244426560322 1107 92-93 3.0339717413651 1107 94-95 2.998654119915635 1107 96-97 3.2443163422992356 1107 98-99 3.4473516615447473 1107 100-101 3.2892856239111268 1108 1 0.20860775929067188 1108 2 0.2157411375992595 1108 3 0.17478106157278717 1108 4 0.12781870047393795 1108 5 0.013297947641362384 1108 6 0.07708608694586871 1108 7 0.1964876042117112 1108 8 0.170269325252427 1108 9 0.1767195078305619 1108 10-11 0.16791230910461508 1108 12-13 0.22969448867532805 1108 14-15 0.3235479835511086 1108 16-17 0.29857632541470736 1108 18-19 0.26265569386081467 1108 20-21 0.22948643215389808 1108 22-23 0.28537354270766 1108 24-25 0.2917036995692257 1108 26-27 0.38001959436932964 1108 28-29 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2316 54-55 0.28187533061863945 2316 56-57 0.42279741240865576 2316 58-59 0.5368005479857061 2316 60-61 0.625718476440241 2316 62-63 0.5936029630145185 2316 64-65 1.0135300723465335 2316 66-67 1.0678358134463082 2316 68-69 1.2577512071713564 2316 70-71 1.3882459543301202 2316 72-73 1.4880011910175845 2316 74-75 1.8115951373081032 2316 76-77 1.9053665138355633 2316 78-79 1.9131239535027795 2316 80-81 2.0433666138506226 2316 82-83 2.2708847883172503 2316 84-85 2.451989151169517 2316 86-87 2.6278082596161063 2316 88-89 2.91398181971644 2316 90-91 3.162975383774384 2316 92-93 3.2235638728039433 2316 94-95 3.692433797462453 2316 96-97 4.182226317837738 2316 98-99 4.550467361438084 2316 100-101 4.842247697176838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 16.0 5 48.0 6 82.0 7 254.0 8 588.0 9 1164.0 10 2134.0 11 3389.0 12 5258.0 13 7843.0 14 10304.0 15 14670.0 16 19524.0 17 26846.0 18 27996.0 19 23970.0 20 23121.0 21 24929.0 22 27491.0 23 29911.0 24 33172.0 25 37364.0 26 42206.0 27 49354.0 28 58964.0 29 70661.0 30 84420.0 31 100917.0 32 122878.0 33 152520.0 34 196877.0 35 269497.0 36 366884.0 37 427612.0 38 200016.0 39 7542.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6131060372388 18.159643204392424 14.932558408765424 23.29469234960335 2 16.044413118436196 19.43543551810649 39.47133717477281 25.0488141886845 3 19.92367904719844 22.832044113462143 29.28649583845574 27.95778100088368 4 13.52117944082536 16.27378014249566 35.31744569989123 34.887594716787746 5 14.381150141877253 36.565022414255644 33.55439600652611 15.499431437340991 6 33.721038489593326 34.902328902532915 16.355304371620534 15.021328236253225 7 29.755419493093115 32.19102392535077 19.269772031958755 18.783784549597357 8 27.272856069011553 34.45121025636459 18.629734859601193 19.646198815022668 9 26.440293486893395 16.7014378850032 19.425696051786854 37.432572576316545 10-11 25.82315495981678 25.952282068656764 25.988672435693484 22.235890535832976 12-13 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28.309168047337756 23.0366776135365 38-39 22.88287467715949 25.617526090371058 28.30007772461198 23.199521507857472 40-41 23.233180733148853 25.48370453090371 28.27746798260289 23.005646753344543 42-43 23.01069575325383 25.448334894897524 28.076843126455937 23.464126225392715 44-45 22.98556208255923 25.708795424751013 28.263832989486648 23.041809503203105 46-47 23.478976463037764 25.994992376843985 27.560284460196964 22.96574669992129 48-49 23.269919374490474 26.074042050910894 27.36442260752564 23.291615967072993 50-51 23.21442305279153 26.299387071259545 27.436515203401505 23.049674672547415 52-53 23.541369654076977 26.055927824827542 27.28419378943133 23.118508731664154 54-55 23.16991670289512 26.17250653124107 27.365171463799477 23.292405302064335 56-57 23.26051278044926 26.407086043805673 27.163410794097476 23.168990381647596 58-59 23.215617174957924 26.02724865347546 27.21843611284495 23.538698058721668 60-61 22.966733780380288 26.21733671087752 27.530547264164916 23.285382244577267 62-63 22.971449550969584 26.181168976787074 27.653440650509182 23.193940821734156 64-65 22.778750616288473 26.330717598608178 27.394457815914127 23.49607396918922 66-67 22.790230945297658 26.34565957755997 27.253071171517064 23.61103830562531 68-69 23.123372743699846 25.943869372607693 27.472134247835182 23.46062363585728 70-71 23.07592512084921 26.154635177462744 27.047089701647565 23.72235000004048 72-73 23.027096869253285 26.198949738770487 27.05444754016806 23.719505851808165 74-75 22.999035796949038 26.7040123314365 26.742527831142223 23.55442404047224 76-77 23.133627532652156 26.25127811550521 27.084492036621903 23.530602315220726 78-79 23.17121202185527 26.371317752265394 27.044357388216 23.41311283766334 80-81 23.35020284909626 26.46095369940284 26.7044130936587 23.4844303578422 82-83 23.22792731818211 26.399640703631498 26.899421304542127 23.47301067364426 84-85 23.116460017362066 26.56347121445589 26.77332184768139 23.546746920500656 86-87 23.322398341616214 26.43231322436393 26.70105980633568 23.544228627684177 88-89 23.16619266088469 26.644083590170798 26.32221706793219 23.867506681012323 90-91 23.154005908832666 26.521477859891 26.570336947569988 23.754179283706346 92-93 23.195944518250638 26.29452962515898 26.63129231543931 23.87823354115107 94-95 23.07342945010641 26.099883320025945 26.779339245253613 24.04734798461403 96-97 23.23741824049766 25.80192974385244 26.831426007966623 24.129226007683275 98-99 22.985799256325002 26.140002121084798 26.464337845091183 24.409860777499016 100-101 23.48884851811463 25.35475548021117 26.57840687101942 24.577989130654775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 723.0 1 512.0 2 415.0 3 555.5 4 467.0 5 456.0 6 930.0 7 2087.5 8 3409.5 9 4034.0 10 3444.0 11 2619.5 12 2502.0 13 2593.5 14 2722.0 15 2864.0 16 3036.0 17 3213.0 18 3480.0 19 3832.0 20 4186.5 21 4856.0 22 5693.0 23 6672.5 24 8177.5 25 10059.0 26 12512.5 27 15330.5 28 17694.0 29 20008.0 30 22625.5 31 26476.0 32 32373.0 33 34980.0 34 35161.0 35 37614.5 36 41514.5 37 45508.0 38 49547.0 39 53393.0 40 57564.0 41 62791.0 42 66414.5 43 69613.5 44 73908.5 45 79815.0 46 85193.5 47 90702.0 48 96079.0 49 97139.0 50 95269.5 51 93994.0 52 95578.5 53 99371.5 54 101764.0 55 97756.5 56 90519.0 57 83306.0 58 77244.0 59 69518.0 60 58047.5 61 48467.0 62 40111.0 63 30304.0 64 21579.5 65 15815.5 66 11733.0 67 8896.0 68 6742.5 69 5019.0 70 3742.5 71 2594.5 72 1772.0 73 1330.0 74 977.0 75 690.5 76 465.0 77 276.5 78 159.5 79 95.5 80 73.5 81 37.5 82 18.0 83 16.0 84 8.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014288987279158238 3 0.0031168612478617115 4 0.0017405848527019948 5 0.11366423875319073 6 0.0 7 0.0 8 2.833510225328829E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.0478717504697554E-4 24-25 5.059839688087194E-4 26-27 0.00595037147319054 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011172126031296525 36-37 6.881381975798584E-4 38-39 0.007043296845817375 40-41 0.01026135488744083 42-43 0.006253961854475772 44-45 0.003987153674212709 46-47 2.0239358752348775E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.0239358752348775E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0239358752348775E-5 68-69 7.28616915084556E-4 70-71 0.0 72-73 4.047871750469755E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.071807625704633E-5 82-83 0.0020239358752348778 84-85 0.004270504696745592 86-87 0.0031168612478617115 88-89 0.0 90-91 0.0026108772790529922 92-93 0.0 94-95 6.071807625704633E-5 96-97 1.0119679376174389E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2470434.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.54955091703256 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4990380376404 38.4039195459129 2 13.941047238458248 14.373094489113193 3 4.683763306983657 7.243376852300486 4 2.126325259447322 4.384444489122086 5 1.2032689859747745 3.1013987929696385 6 0.7665326160774478 2.370864727323034 7 0.5002616865079543 1.8051785696337659 8 0.36636007279027144 1.510855778101588 9 0.27056400885344695 1.255270783563649 >10 1.4906359605328043 13.921380333242228 >50 0.10319777450341654 3.6256175884834727 >100 0.043192416385708886 4.003246244971703 >500 0.0032205144544367997 1.1089873733045947 >1k 0.002356473991049014 1.8622144478691218 >5k 1.5709826606993428E-4 0.6004922866253333 >10k+ 7.854913303496714E-5 0.42965769746334537 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10611 0.4295196714423457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9127 0.3694492546653746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.23085012592929016 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2595 0.10504227192469015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.095743500939511E-5 2 0.0 0.0 0.0 0.0 8.095743500939511E-5 3 0.0 0.0 0.0 0.0 8.095743500939511E-5 4 0.0 0.0 0.0 0.0 8.095743500939511E-5 5 0.0 0.0 0.0 0.0 1.2143615251409267E-4 6 4.047871750469756E-5 0.0 0.0 0.0 1.2143615251409267E-4 7 4.047871750469756E-5 0.0 0.0 0.0 1.2143615251409267E-4 8 4.047871750469756E-5 0.0 0.0 0.0 1.2143615251409267E-4 9 4.047871750469756E-5 0.0 0.0 0.0 1.2143615251409267E-4 10-11 4.047871750469756E-5 0.0 0.0 0.0 1.6191487001879023E-4 12-13 4.047871750469756E-5 0.0 0.0 0.0 3.845478162946268E-4 14-15 1.2143615251409267E-4 0.0 0.0 0.0 4.250265337993243E-4 16-17 1.2143615251409267E-4 0.0 0.0 0.0 4.452658925516731E-4 18-19 1.2143615251409267E-4 0.0 0.0 4.047871750469756E-5 5.262233275610683E-4 20-21 1.2143615251409267E-4 0.0 0.0 1.011967937617439E-4 5.869414038181145E-4 22-23 1.6191487001879023E-4 0.0 0.0 2.4287230502818534E-4 6.071807625704633E-4 24-25 2.4287230502818534E-4 0.0 0.0 5.667020450657658E-4 6.274201213228121E-4 26-27 2.4287230502818534E-4 0.0 0.0 0.0010119679376174389 6.881381975798584E-4 28-29 3.440690987899292E-4 0.0 0.0 0.003379972911642246 7.28616915084556E-4 30-31 3.64308457542278E-4 0.0 0.0 0.011556673847591152 7.28616915084556E-4 32-33 3.845478162946268E-4 0.0 0.0 0.028355341612040636 7.690956325892536E-4 34-35 4.0478717504697554E-4 0.0 0.0 0.0529461624961444 7.690956325892536E-4 36-37 4.0478717504697554E-4 0.0 0.0 0.09239267270447217 7.690956325892536E-4 38-39 4.857446100563707E-4 0.0 0.0 0.14940694630983867 7.690956325892536E-4 40-41 4.857446100563707E-4 0.0 0.0 0.21168345319081588 7.690956325892536E-4 42-43 5.059839688087194E-4 0.0 0.0 0.27393972071304074 7.893349913416024E-4 44-45 5.667020450657658E-4 0.0 0.0 0.35242795395464926 8.095743500939511E-4 46-47 6.071807625704633E-4 0.0 0.0 0.4432217173176859 8.095743500939511E-4 48-49 6.071807625704633E-4 0.0 0.0 0.5369097089823084 8.095743500939511E-4 50-51 6.071807625704633E-4 0.0 0.0 0.6351313170074571 8.095743500939511E-4 52-53 6.071807625704633E-4 0.0 0.0 0.7332719675975963 8.905317851033462E-4 54-55 6.071807625704633E-4 0.0 0.0 0.8400143456574837 8.905317851033462E-4 56-57 6.476594800751609E-4 0.0 0.0 0.9649316678769804 9.10771143855695E-4 58-59 6.476594800751609E-4 0.0 0.0 1.092986090703091 9.310105026080437E-4 60-61 6.476594800751609E-4 0.0 0.0 1.2332246074981157 9.310105026080437E-4 62-63 6.476594800751609E-4 0.0 0.0 1.3643756522133357 9.714892201127414E-4 64-65 6.881381975798584E-4 0.0 0.0 1.5019020949355457 9.714892201127414E-4 66-67 8.095743500939511E-4 0.0 0.0 1.6621775768953957 9.714892201127414E-4 68-69 8.095743500939511E-4 2.023935875234878E-5 0.0 1.8364789344706234 9.714892201127414E-4 70-71 8.095743500939511E-4 4.047871750469756E-5 0.0 2.0206166203994926 9.9172857886509E-4 72-73 8.905317851033462E-4 4.047871750469756E-5 0.0 2.2093284014063928 0.001072686013874485 74-75 9.310105026080437E-4 4.047871750469756E-5 0.0 2.3958138529505346 0.0011334040901315315 76-77 9.310105026080437E-4 4.047871750469756E-5 0.0 2.594847706921132 0.0011334040901315315 78-79 9.310105026080437E-4 4.047871750469756E-5 0.0 2.8182902275470623 0.0011334040901315315 80-81 0.0010322072963697877 4.047871750469756E-5 0.0 3.051083331916578 0.0011334040901315315 82-83 0.001092925372626834 4.047871750469756E-5 0.0 3.2912233235131962 0.0012143615251409266 84-85 0.0011131647313791827 4.047871750469756E-5 0.0 3.5346825699452 0.0012143615251409266 86-87 0.0011334040901315315 4.047871750469756E-5 0.0 3.785731575909334 0.0012750796013979728 88-89 0.0011334040901315315 4.047871750469756E-5 0.0 4.060258238026194 0.0013357976776550192 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6380 0.0 44.21015 1 GTATCAA 10250 0.0 37.72721 1 ATCAACG 12475 0.0 30.72456 3 TCAACGC 12510 0.0 30.638601 4 TATCAAC 12670 0.0 30.446756 2 CAACGCA 12810 0.0 29.8097 5 AACGCAG 13225 0.0 28.874537 6 TAAGGTG 1260 0.0 26.419142 5 ACGCAGA 15020 0.0 25.360575 7 CGCAGAG 15210 0.0 24.981321 8 GAGTACT 9515 0.0 22.861385 12-13 GTACATG 8785 0.0 22.631977 1 TACATGG 8460 0.0 22.602293 2 GCAGAGT 16575 0.0 22.465101 9 GTAAGGT 1340 0.0 22.357689 4 GTACTTT 9735 0.0 21.881262 14-15 AGAGTAC 13440 0.0 21.044003 10-11 AGTACTT 9755 0.0 20.521835 12-13 ACATGGG 8810 0.0 20.511568 3 CAGAGTA 15530 0.0 20.44447 10-11 >>END_MODULE