FastQCFastQC Report
Thu 26 May 2016
SRR938186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463322
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108200.43924424009528595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.3659286118501763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.22295095809642426No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25460.10335636185606267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.044.465481
GTATCAA99700.039.1158751
TATCAAC123650.031.5553952
ATCAACG124050.031.225683
TCAACGC124600.030.9734124
CAACGCA125950.030.7168925
AACGCAG131500.029.4162926
ACGCAGA148400.025.9313857
CGCAGAG151000.025.4529048
GTAAGGT14300.023.2655354
GTACATG85500.023.1690441
GTGTAAG9300.023.0970061
GCAGAGT161400.023.0470399
TACATGG84700.023.0051482
GAGTACT95050.022.6661512-13
AGGTAAG13900.022.5659562
TAAGGTG15400.022.5295875
GTACTTT98100.021.79194614-15
AGAGTAC133300.021.59641810-11
ACATGGG87600.021.1055453
Produced by FastQC (version 0.11.5)