##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2463322 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815236903660992 33.0 31.0 34.0 30.0 34.0 2 32.00530056565889 34.0 31.0 34.0 30.0 34.0 3 32.086858315721614 34.0 31.0 34.0 30.0 34.0 4 35.604564486494255 37.0 35.0 37.0 33.0 37.0 5 35.61104516583703 37.0 35.0 37.0 33.0 37.0 6 35.63424676108117 37.0 35.0 37.0 33.0 37.0 7 35.57695948804095 37.0 35.0 37.0 33.0 37.0 8 35.543300063897455 37.0 35.0 37.0 33.0 37.0 9 37.17356683373104 39.0 37.0 39.0 34.0 39.0 10-11 37.08698619181739 39.0 37.0 39.0 33.0 39.0 12-13 36.98144781721594 39.0 37.0 39.0 33.0 39.0 14-15 38.156590977549826 40.0 38.0 41.0 33.0 41.0 16-17 38.00017212528448 40.0 38.0 41.0 33.0 41.0 18-19 38.13808588564548 40.0 38.0 41.0 33.0 41.0 20-21 38.11397637012132 40.0 38.0 41.0 33.0 41.0 22-23 38.02399422406003 40.0 38.0 41.0 33.0 41.0 24-25 37.909233547217944 40.0 38.0 41.0 33.0 41.0 26-27 37.72959950018715 40.0 38.0 41.0 32.0 41.0 28-29 37.51258909716229 40.0 37.5 41.0 32.0 41.0 30-31 37.29763039505188 40.0 37.0 41.0 31.5 41.0 32-33 37.03868170706063 40.0 36.5 41.0 31.0 41.0 34-35 36.80493394692208 39.5 36.0 41.0 30.0 41.0 36-37 36.53937670349228 39.0 35.5 41.0 30.0 41.0 38-39 36.240850769814095 39.0 35.0 40.5 30.0 41.0 40-41 36.02410850063451 39.0 35.0 40.0 29.0 41.0 42-43 35.843379184694484 39.0 35.0 40.0 28.5 41.0 44-45 35.560199194421195 39.0 35.0 40.0 27.0 41.0 46-47 35.16507829670664 38.5 35.0 40.0 25.5 41.0 48-49 34.759427918883524 38.0 34.0 40.0 24.0 41.0 50-51 34.03625916546842 37.5 33.5 39.5 23.0 40.5 52-53 34.15370158671907 38.0 33.5 40.0 23.0 40.5 54-55 34.24685018848531 38.0 34.0 40.0 23.0 41.0 56-57 33.92209098120343 38.0 33.5 40.0 22.0 41.0 58-59 33.57136196567075 37.0 33.0 40.0 20.5 41.0 60-61 33.124274455389916 37.0 33.0 40.0 18.5 41.0 62-63 32.56361754573702 36.0 32.0 39.0 15.0 41.0 64-65 32.02306925363391 35.5 31.0 39.0 10.0 40.0 66-67 31.470014273408026 35.0 31.0 38.5 7.5 40.0 68-69 30.840798726272894 35.0 30.0 38.0 4.0 40.0 70-71 30.276613654244144 35.0 29.5 37.0 2.0 39.5 72-73 29.62070163786951 34.0 29.0 36.5 2.0 39.0 74-75 28.93441884577006 34.0 28.0 36.0 2.0 39.0 76-77 28.26759108228644 34.0 26.5 35.0 2.0 37.5 78-79 27.546330321411492 33.0 26.0 35.0 2.0 37.0 80-81 26.88978136029313 33.0 25.0 35.0 2.0 36.5 82-83 26.160040587466845 32.0 23.0 35.0 2.0 36.0 84-85 25.504088990395896 32.0 20.0 35.0 2.0 35.5 86-87 24.816796180117745 31.0 18.5 34.0 2.0 35.0 88-89 23.994599366221713 31.0 9.5 34.0 2.0 35.0 90-91 23.164048183712886 30.0 3.5 34.0 2.0 35.0 92-93 22.27300775132118 29.5 2.0 34.0 2.0 35.0 94-95 21.30855872679252 29.0 2.0 34.0 2.0 35.0 96-97 20.096122228437856 28.0 2.0 34.0 2.0 35.0 98-99 18.60256292112846 25.5 2.0 33.0 2.0 35.0 100-101 15.000647296618144 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3750777417281448 1101 2 0.33951923607862255 1101 3 0.34553555986671824 1101 4 0.38147405017057423 1101 5 0.3472267018832369 1101 6 0.40850156281022976 1101 7 0.27230515459297067 1101 8 0.30693313243722997 1101 9 0.4306493823063491 1101 10-11 0.40603704142818486 1101 12-13 0.5390886684637124 1101 14-15 0.6516043575585115 1101 16-17 0.7019510781000875 1101 18-19 0.5730529539584879 1101 20-21 0.5624568121711206 1101 22-23 0.5916360998630665 1101 24-25 0.616766679116914 1101 26-27 0.7018790048531969 1101 28-29 0.7255037848207238 1101 30-31 0.8462135433120537 1101 32-33 0.6985822798747563 1101 34-35 0.8155916675557151 1101 36-37 0.862187955802014 1101 38-39 0.9785444878238181 1101 40-41 1.0963959589887935 1101 42-43 1.0750488251678973 1101 44-45 1.0237552851196412 1101 46-47 1.1004119568661395 1101 48-49 1.133085104006554 1101 50-51 1.139797578053809 1101 52-53 1.1441777148606107 1101 54-55 1.214034454728683 1101 56-57 1.1763743379663296 1101 58-59 1.2323001812532581 1101 60-61 1.204578191421838 1101 62-63 1.1657119942474026 1101 64-65 1.163117953247017 1101 66-67 1.1716719044709798 1101 68-69 1.5469326591551429 1101 70-71 1.8702150460240325 1101 72-73 1.9334399584001964 1101 74-75 2.0635976479015525 1101 76-77 2.210397394806378 1101 78-79 2.3205824152668058 1101 80-81 2.5704916209172133 1101 82-83 2.868274323860497 1101 84-85 2.942977743933181 1101 86-87 3.0427628023046083 1101 88-89 3.3682622100620385 1101 90-91 3.601926853806315 1101 92-93 3.9204371728044656 1101 94-95 3.9979975629484343 1101 96-97 4.258050766729827 1101 98-99 4.649104114434035 1101 100-101 4.270815642328364 1102 1 0.33793557136588603 1102 2 0.24734767783674272 1102 3 0.11588444609176207 1102 4 0.08132633632369846 1102 5 0.1494166076985053 1102 6 0.17136398032162958 1102 7 0.0657165366761916 1102 8 0.09646776127629408 1102 9 0.28773456892497506 1102 10-11 0.16907160223339446 1102 12-13 0.27645476235199595 1102 14-15 0.36599049810194373 1102 16-17 0.442780722508509 1102 18-19 0.33665139188553894 1102 20-21 0.4404314306423771 1102 22-23 0.4827964436947809 1102 24-25 0.4178358889556577 1102 26-27 0.3922983090570611 1102 28-29 0.4036556246168814 1102 30-31 0.49665462619140754 1102 32-33 0.4831099504387595 1102 34-35 0.4551105721440223 1102 36-37 0.6532516797986716 1102 38-39 0.757706208801892 1102 40-41 0.8282035694477372 1102 42-43 0.9751747368117236 1102 44-45 0.9518720296429208 1102 46-47 0.936961605305612 1102 48-49 1.0177659585744863 1102 50-51 0.9646884395976656 1102 52-53 0.8848469797538456 1102 54-55 0.9823538930788374 1102 56-57 1.1167915835089417 1102 58-59 1.0677381526131668 1102 60-61 1.0810275379635428 1102 62-63 1.0636649399171816 1102 64-65 1.2141810851334895 1102 66-67 1.2816647050384624 1102 68-69 1.4775352861637607 1102 70-71 1.6761609302896296 1102 72-73 1.6634910406482213 1102 74-75 1.7147336760028686 1102 76-77 2.00818872576421 1102 78-79 2.136628973949829 1102 80-81 2.185241301432594 1102 82-83 2.4649208234893507 1102 84-85 2.5518841595451285 1102 86-87 2.7465299759509207 1102 88-89 3.1722219763706576 1102 90-91 3.1814778382189175 1102 92-93 3.408693882765757 1102 94-95 3.813103215404851 1102 96-97 3.917381344986211 1102 98-99 3.891697345320541 1102 100-101 3.8041876196744635 1103 1 0.550756619777065 1103 2 0.5648787680660519 1103 3 0.47936968264292545 1103 4 0.4215814600841483 1103 5 0.47211713634805363 1103 6 0.41882856061463514 1103 7 0.44455074127893823 1103 8 0.4768103904865555 1103 9 0.5098206528668427 1103 10-11 0.6058074891408083 1103 12-13 0.5638147903148578 1103 14-15 0.7739001944964485 1103 16-17 0.7544618776215657 1103 18-19 0.6660137787478533 1103 20-21 0.7261234531416392 1103 22-23 0.6840658708532459 1103 24-25 0.7369747037395342 1103 26-27 0.7054100843485216 1103 28-29 0.8655542349316434 1103 30-31 0.8673714690045031 1103 32-33 0.9106347742572893 1103 34-35 0.9540382495392876 1103 36-37 1.1144370828466421 1103 38-39 1.1413481663706762 1103 40-41 1.1533878818318186 1103 42-43 1.1961519324036445 1103 44-45 1.3005014099972314 1103 46-47 1.3620123417373833 1103 48-49 1.6232399090441376 1103 50-51 1.5755172273298896 1103 52-53 1.5307891633526722 1103 54-55 1.7169631022260248 1103 56-57 1.837626254470969 1103 58-59 1.8186371091926858 1103 60-61 1.9259462545315884 1103 62-63 2.0164351981577866 1103 64-65 2.186031068848294 1103 66-67 2.1757240448176454 1103 68-69 2.4274669678001892 1103 70-71 2.513741614417203 1103 72-73 2.7047377853623153 1103 74-75 2.73351059505028 1103 76-77 2.922228616772589 1103 78-79 2.999116421268287 1103 80-81 3.157692225062288 1103 82-83 3.301432923363137 1103 84-85 3.3187254305657596 1103 86-87 3.348761140620141 1103 88-89 3.7525481568367454 1103 90-91 3.984419780226812 1103 92-93 4.193069772084403 1103 94-95 4.391971672648161 1103 96-97 4.774639773916185 1103 98-99 4.942862519216948 1103 100-101 4.4898446418629785 1104 1 0.23346202208960776 1104 2 0.24289151655015928 1104 3 0.2940450243544319 1104 4 0.2326873643272549 1104 5 0.26443904851711153 1104 6 0.20685308064783925 1104 7 0.23182055312401673 1104 8 0.3041004487056682 1104 9 0.3973484204322517 1104 10-11 0.36618375583370977 1104 12-13 0.36021553561616315 1104 14-15 0.4394673777649629 1104 16-17 0.550437802905023 1104 18-19 0.5626998805527634 1104 20-21 0.4508176431399633 1104 22-23 0.3434941998973926 1104 24-25 0.43554408079675255 1104 26-27 0.47162034458261104 1104 28-29 0.4877962490057186 1104 30-31 0.5228422187762263 1104 32-33 0.49447081487431177 1104 34-35 0.5786132137672411 1104 36-37 0.7291669672196477 1104 38-39 0.8067326058326358 1104 40-41 0.8669230489032458 1104 42-43 0.7264205204909118 1104 44-45 0.7800945148382326 1104 46-47 0.8906709719916464 1104 48-49 1.006821748063885 1104 50-51 1.0571004489025526 1104 52-53 1.0337160848164189 1104 54-55 1.2687346168032363 1104 56-57 1.3313680881745356 1104 58-59 1.2815107738899414 1104 60-61 1.2372545362133636 1104 62-63 1.4250015891768868 1104 64-65 1.3527428281425529 1104 66-67 1.4063926843407124 1104 68-69 1.537716931984992 1104 70-71 1.6949833402436703 1104 72-73 1.8533600653491256 1104 74-75 1.8605742839637927 1104 76-77 2.018470308078385 1104 78-79 2.139603689181495 1104 80-81 2.265498341434924 1104 82-83 2.4735456575211643 1104 84-85 2.7510426910742787 1104 86-87 2.9109501309422683 1104 88-89 3.139164794419745 1104 90-91 3.0954956514854217 1104 92-93 3.4158271366382316 1104 94-95 3.6138604107967858 1104 96-97 3.7387926452593696 1104 98-99 3.821324079585832 1104 100-101 4.048759562215158 1105 1 0.29517436334644387 1105 2 0.15908775045269152 1105 3 0.13654929937365523 1105 4 0.1323506547594988 1105 5 0.170133590901969 1105 6 0.23026941077496588 1105 7 0.20245521973627945 1105 8 0.2344169977800803 1105 9 0.3379753849964544 1105 10-11 0.21809737685468633 1105 12-13 0.20582916186535982 1105 14-15 0.33033484005489555 1105 16-17 0.4188067412083001 1105 18-19 0.26401758110886675 1105 20-21 0.39784534118398085 1105 22-23 0.40543400023071996 1105 24-25 0.3280086979195218 1105 26-27 0.3676409424604614 1105 28-29 0.37086124332482484 1105 30-31 0.4170334208440565 1105 32-33 0.5239917194313932 1105 34-35 0.49212281646143197 1105 36-37 0.5204185117735776 1105 38-39 0.6071805424106032 1105 40-41 0.7741897208406243 1105 42-43 0.5921578194438624 1105 44-45 0.6085466964688564 1105 46-47 0.6241303774802489 1105 48-49 0.5857347455250164 1105 50-51 0.6946476781417701 1105 52-53 0.7222995838160458 1105 54-55 0.8582122674745492 1105 56-57 0.8302201132138478 1105 58-59 0.888167402815732 1105 60-61 1.0775285327474435 1105 62-63 1.0047974849033992 1105 64-65 1.193258948248598 1105 66-67 1.331492020357853 1105 68-69 1.4819405234601994 1105 70-71 1.657406845772183 1105 72-73 1.6229591635464118 1105 74-75 1.7930305742034882 1105 76-77 1.847619850227332 1105 78-79 1.8163521433523933 1105 80-81 1.9545987638999094 1105 82-83 2.137533609681757 1105 84-85 2.428917442256047 1105 86-87 2.586190851823364 1105 88-89 2.7104758449459467 1105 90-91 2.8898477804513725 1105 92-93 3.229129778670245 1105 94-95 3.279389569552066 1105 96-97 3.419573995081546 1105 98-99 3.831023208769839 1105 100-101 3.9544874185082026 1106 1 0.17833722723314693 1106 2 0.1350908975809375 1106 3 0.13222199803612256 1106 4 0.17286993092000102 1106 5 0.09027521167300989 1106 6 -0.07342846491346933 1106 7 0.10410746206515142 1106 8 0.11010543208377754 1106 9 0.011854220559008866 1106 10-11 0.07568982374689881 1106 12-13 0.28411397697157525 1106 14-15 0.2431987267582869 1106 16-17 0.07242593869060698 1106 18-19 0.23038264798534414 1106 20-21 0.14764864566863167 1106 22-23 0.22762125436132408 1106 24-25 0.11341389068113017 1106 26-27 0.16268421547383838 1106 28-29 0.19265510642474482 1106 30-31 0.12258023437670573 1106 32-33 0.289333969628089 1106 34-35 0.31116294234655584 1106 36-37 0.20786933789474205 1106 38-39 0.1378650428039947 1106 40-41 0.30074361541183237 1106 42-43 0.2552184016625887 1106 44-45 0.36307069332172404 1106 46-47 0.5145709754346157 1106 48-49 0.4930911577988155 1106 50-51 0.510934066812105 1106 52-53 0.5960210629663223 1106 54-55 0.22052540673497845 1106 56-57 0.4922972178558567 1106 58-59 0.699339708087166 1106 60-61 0.663484472952014 1106 62-63 0.4507062181842798 1106 64-65 0.7150921504515821 1106 66-67 0.8017909975411719 1106 68-69 0.605662002610476 1106 70-71 0.8485948866848467 1106 72-73 1.236649171414232 1106 74-75 1.263526246902149 1106 76-77 1.4975018329181289 1106 78-79 1.6861397121958248 1106 80-81 1.7897679220431044 1106 82-83 1.881042657675465 1106 84-85 1.9961873085030994 1106 86-87 1.948897382114474 1106 88-89 2.186085601043505 1106 90-91 2.502751006257821 1106 92-93 2.8203964977890514 1106 94-95 2.9776586490170516 1106 96-97 2.931965812548107 1106 98-99 3.1463261198634633 1106 100-101 3.30027813447988 1107 1 0.30256630529434503 1107 2 0.12396166585277513 1107 3 0.1325943922142372 1107 4 0.12324147022626164 1107 5 0.16506195114076405 1107 6 0.21182607543093468 1107 7 0.19974857546225877 1107 8 0.1800678447181241 1107 9 0.058801833111886026 1107 10-11 0.10950420497115232 1107 12-13 0.13509279024746235 1107 14-15 0.21406881833155467 1107 16-17 0.2736995177810968 1107 18-19 0.18672584214053956 1107 20-21 0.2523492260516349 1107 22-23 0.2562302908667533 1107 24-25 0.272240682545025 1107 26-27 0.2686964992325045 1107 28-29 0.3188254934837218 1107 30-31 0.3414132563067156 1107 32-33 0.43774564771754854 1107 34-35 0.3988233020586378 1107 36-37 0.3703119377490225 1107 38-39 0.4096063087882271 1107 40-41 0.416709457132157 1107 42-43 0.5852352321397021 1107 44-45 0.47229729525409425 1107 46-47 0.611502495399769 1107 48-49 0.6972989720474629 1107 50-51 0.5370490521758029 1107 52-53 0.5211880554341448 1107 54-55 0.528369185952478 1107 56-57 0.7421917086716903 1107 58-59 0.6507124223173051 1107 60-61 0.6558842122310935 1107 62-63 0.7076848220915579 1107 64-65 0.8318556476033621 1107 66-67 1.0825804072086633 1107 68-69 1.2394203522338678 1107 70-71 1.3596286577225705 1107 72-73 1.4772575042251432 1107 74-75 1.5764252433425803 1107 76-77 1.8257891976421554 1107 78-79 1.9102534425476456 1107 80-81 2.1739766541541634 1107 82-83 2.296810709044564 1107 84-85 2.3801342548558395 1107 86-87 2.3798175121869747 1107 88-89 2.793072363727781 1107 90-91 3.0822252180734715 1107 92-93 3.2768737718456684 1107 94-95 3.545057120986101 1107 96-97 3.6088875419808666 1107 98-99 3.742893944346573 1107 100-101 3.763326298560086 1108 1 0.2875857709213214 1108 2 0.12929507370478177 1108 3 0.16924489003984178 1108 4 0.11356468831513666 1108 5 0.136554709380043 1108 6 0.08018793734459706 1108 7 0.02216796484066208 1108 8 0.17039106280699912 1108 9 0.1586493930008004 1108 10-11 0.21508544919156236 1108 12-13 0.22056882056553917 1108 14-15 0.37020444725822443 1108 16-17 0.32351857712922794 1108 18-19 0.23756402037570723 1108 20-21 0.2145785243794407 1108 22-23 0.22709251563122734 1108 24-25 0.3263286210420304 1108 26-27 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2316 52-53 -1.721011469338471 2316 54-55 -1.609401947238993 2316 56-57 -1.5670719300033227 2316 58-59 -1.4014211980778768 2316 60-61 -1.717213960616327 2316 62-63 -1.912674992929304 2316 64-65 -1.8345149284859303 2316 66-67 -1.9541097296931476 2316 68-69 -2.1498066303294294 2316 70-71 -2.138188639879136 2316 72-73 -2.030012030720197 2316 74-75 -2.0991089588029332 2316 76-77 -2.3668552533453635 2316 78-79 -3.0327228209991333 2316 80-81 -3.00863672942571 2316 82-83 -2.963625110744111 2316 84-85 -2.8994265335533065 2316 86-87 -2.9084711387518354 2316 88-89 -3.133032898950688 2316 90-91 -3.370564365075115 2316 92-93 -3.6799165839693835 2316 94-95 -3.700668297356259 2316 96-97 -3.841146670747289 2316 98-99 -3.6645372504277987 2316 100-101 -3.1180503766110927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7430.0 3 2287.0 4 2426.0 5 2651.0 6 3255.0 7 3872.0 8 4781.0 9 5988.0 10 7536.0 11 9311.0 12 11722.0 13 14082.0 14 15990.0 15 19607.0 16 23396.0 17 28611.0 18 28371.0 19 24338.0 20 24795.0 21 26591.0 22 29109.0 23 32433.0 24 36224.0 25 40668.0 26 46085.0 27 52564.0 28 60775.0 29 70535.0 30 83613.0 31 99748.0 32 122388.0 33 153848.0 34 200909.0 35 269164.0 36 347776.0 37 367933.0 38 173363.0 39 9147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.457268516215024 17.759603847656553 14.489927756520633 23.293199879607794 2 15.856569757194297 19.11592341788037 40.03642126176442 24.991085563160915 3 19.879715287458286 22.909863232699376 29.04164595173339 28.168775528108945 4 13.313132010634126 15.939452898896578 35.5733513141353 35.17406377633399 5 14.521060997094137 36.522684574229494 33.363656900284084 15.592597528392286 6 33.64138580545239 34.73378976454829 16.561456711964436 15.063367718034884 7 29.75539460815373 32.24730391440111 19.291633767761347 18.705667709683812 8 27.23815480336354 34.232917797899105 18.76488708658827 19.76404031214908 9 26.674061314928753 16.741973753737092 19.457415314557533 37.126549616776614 10-11 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28.20688824638357 22.828594794569675 36-37 23.3734074625526 25.295438567419943 28.257337887008582 23.073816083018876 38-39 22.91564303204298 25.663701002586663 28.19577037557408 23.224885589796273 40-41 23.288255016796764 25.515526396681338 28.262821311930065 22.933397274591837 42-43 23.019062527282212 25.451285893242538 28.05817634005161 23.471475239423636 44-45 23.050375649225153 25.755947373378763 28.1896281511172 23.00404882627888 46-47 23.525327166465832 26.016269241428596 27.51651948278715 22.94188410931842 48-49 23.334951516432476 26.100603072679974 27.320148694174968 23.244296716712583 50-51 23.27229570584679 26.292771103415596 27.416037227552643 23.018895963184967 52-53 23.54084252333925 26.093391113198976 27.26604818444515 23.09971817901662 54-55 23.142775995431382 26.19540990369239 27.374269125756555 23.287544975119673 56-57 23.250091405674116 26.387584825582138 27.23541939927846 23.126904369465286 58-59 23.21493353168702 25.99301525960534 27.244547726589417 23.547503482118223 60-61 22.99426461461503 26.184142596326193 27.54747157633467 23.274121212724104 62-63 23.02471118314925 26.194824541707657 27.647039477557488 23.133424797585604 64-65 22.831065894750218 26.319043053147855 27.500508044563375 23.34938300753855 66-67 22.8298344676836 26.267902353309914 27.381262677196556 23.521000501809926 68-69 23.114472687350943 25.9273860555307 27.65138258333949 23.306758673778866 70-71 23.13061495621725 26.064400395965308 27.19790794537368 23.607076702443763 72-73 23.062493528956775 26.15970230155975 27.202499497542526 23.57530467194095 74-75 23.045044682923898 26.632671062586947 26.927254969669693 23.395029284819458 76-77 23.303582466645732 26.208823922871723 27.001068384092914 23.486525226389634 78-79 23.342564765514044 26.307023819287505 27.040388767670798 23.31002264752765 80-81 23.496935168246505 26.37458612641119 26.705299266969963 23.423179438372348 82-83 23.43873232931747 26.327147304494524 26.824276246806413 23.409844119381592 84-85 23.332394770492964 26.50364770722361 26.752999560898754 23.410957961384668 86-87 23.610724029725954 26.36342581268653 26.596879022277413 23.428971135310096 88-89 23.42870462188935 26.476740304704077 26.382530396907967 23.712024676498608 90-91 23.471251013453628 26.395601535109403 26.47564229051383 23.657505160923137 92-93 23.5894012765129 26.105336371238323 26.5846732780282 23.720589074220584 94-95 23.50428053281938 25.904233750818022 26.749720962823943 23.841764753538648 96-97 23.693077854543418 25.609392118469042 26.714313791257922 23.983216235729618 98-99 23.551618485244884 25.850853650390622 26.35284568064536 24.244682183719135 100-101 24.514757708176464 24.67750801351116 26.385471435146258 24.422262843166116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 860.0 1 589.0 2 474.0 3 608.5 4 488.0 5 464.0 6 900.0 7 2080.5 8 3508.5 9 4112.5 10 3481.5 11 2610.5 12 2447.0 13 2541.5 14 2637.0 15 2850.5 16 3050.0 17 3267.0 18 3578.5 19 3856.5 20 4224.5 21 4851.0 22 5662.0 23 6748.5 24 8167.5 25 10086.0 26 12331.0 27 15178.0 28 17843.5 29 19932.0 30 22503.0 31 26270.0 32 31825.5 33 34655.5 34 35227.5 35 37788.0 36 41418.0 37 45003.0 38 49186.0 39 52947.5 40 56915.5 41 62471.0 42 66369.5 43 69439.5 44 73588.0 45 79479.5 46 84749.0 47 90147.5 48 95371.0 49 96252.5 50 94759.0 51 94192.0 52 95912.5 53 98621.0 54 100166.5 55 96364.5 56 89408.5 57 82309.0 58 76600.0 59 69004.0 60 57342.0 61 48119.0 62 40490.0 63 31101.5 64 22502.0 65 16787.0 66 12585.0 67 9730.0 68 7362.0 69 5374.5 70 4072.0 71 2890.0 72 2030.5 73 1534.5 74 1107.5 75 770.0 76 529.5 77 365.5 78 241.5 79 141.0 80 84.5 81 55.0 82 36.0 83 30.5 84 23.0 85 17.0 86 14.0 87 11.0 88 8.0 89 6.5 90 4.5 91 2.5 92 3.5 93 3.0 94 2.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46019156245103154 2 0.04156988002380525 3 0.05399212932779393 4 0.05833585702559389 5 0.08484477465796189 6 0.08334273797741425 7 0.050094953075562186 8 0.04209762264129497 9 0.04794338701964258 10-11 0.05157669196312947 12-13 0.04707058192148651 14-15 0.040879755062472545 16-17 0.04126541312909965 18-19 0.04972959280191546 20-21 0.05713828723975184 22-23 0.04952661487211173 24-25 0.06641437863178261 26-27 0.05750364751339858 28-29 0.045852714342664094 30-31 0.04711117750744726 32-33 0.0469284973706239 34-35 0.0510895449316005 36-37 0.0460759900654482 38-39 0.02809214548483714 40-41 0.026184152944682015 42-43 0.025859388256996043 44-45 0.015669896180848464 46-47 0.02839661237954275 48-49 0.027747083004170794 50-51 0.016847168173710136 52-53 0.016684785829867148 54-55 0.017557590928023212 56-57 0.0162585321772793 58-59 0.0154872160440251 60-61 0.018410098233198908 62-63 0.0174155063771606 64-65 0.018166524717434424 66-67 0.00966174945865786 68-69 0.01315296985128213 70-71 0.01948588126115871 72-73 0.017334315205239103 74-75 0.014472326395006417 76-77 0.010412767798931686 78-79 0.014614410945869034 80-81 0.018775458506845633 82-83 0.015406024872103605 84-85 0.01366041467579147 86-87 0.0035521137715653905 88-89 0.029878351267110023 90-91 0.03355225179655766 92-93 0.01788235561570919 94-95 0.033958207656165126 96-97 0.028579292516366112 98-99 0.025717303706133423 100-101 0.15125915328974449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2463322.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.42121059046598 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83071485115268 40.50968587289004 2 13.32251325486645 14.2340957236499 3 4.427457958639763 7.09560491966788 4 2.0340802551434902 4.346521186717167 5 1.107708073621917 2.958755313685791 6 0.6982225252281017 2.2379935535530424 7 0.4710281012674044 1.7614023974283344 8 0.3423532401016278 1.463113962863794 9 0.2506483549296717 1.2050944697566668 >10 1.3829813185865825 13.415275318394729 >50 0.08905956433977881 3.24401846704538 >100 0.037893103447811646 3.6727253863951024 >500 0.003051084956996838 1.1310875086727008 >1k 0.002059482345971506 1.692637943033783 >5k 1.5255424784974117E-4 0.5910928525397351 >10k+ 7.627712392487058E-5 0.4408951237060481 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10820 0.43924424009528595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9014 0.3659286118501763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5492 0.22295095809642426 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2546 0.10335636185606267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.119117192149463E-5 0.0 0.0 0.0 0.0 7 1.2178675788224195E-4 0.0 0.0 0.0 0.0 8 1.2178675788224195E-4 0.0 0.0 0.0 0.0 9 1.2178675788224195E-4 0.0 0.0 0.0 0.0 10-11 1.2178675788224195E-4 0.0 0.0 0.0 0.0 12-13 1.8268013682336292E-4 0.0 0.0 0.0 4.059558596074732E-5 14-15 2.0297792980373657E-4 0.0 0.0 0.0 4.059558596074732E-5 16-17 2.0297792980373657E-4 0.0 0.0 0.0 1.4208455086261562E-4 18-19 2.0297792980373657E-4 0.0 0.0 0.0 2.0297792980373657E-4 20-21 2.0297792980373657E-4 0.0 0.0 0.0 2.0297792980373657E-4 22-23 2.0297792980373657E-4 0.0 0.0 4.059558596074732E-5 2.0297792980373657E-4 24-25 2.0297792980373657E-4 0.0 0.0 3.044668947056049E-4 2.0297792980373657E-4 26-27 2.0297792980373657E-4 0.0 0.0 7.10422754313078E-4 2.0297792980373657E-4 28-29 3.044668947056049E-4 0.0 0.0 0.0029431799821541807 2.0297792980373657E-4 30-31 3.2476468768597854E-4 0.0 0.0 0.011366764069009248 2.2327572278411022E-4 32-33 3.6536027364672584E-4 0.0 0.0 0.028863461618091342 2.435735157644839E-4 34-35 3.856580666270995E-4 0.0 0.0 0.05476344546104813 2.435735157644839E-4 36-37 4.0595585960747315E-4 4.059558596074732E-5 0.0 0.0953184358358347 2.435735157644839E-4 38-39 4.0595585960747315E-4 4.059558596074732E-5 0.0 0.1551157339560155 2.435735157644839E-4 40-41 4.0595585960747315E-4 6.0893378941120976E-5 0.0 0.22256530002979716 2.638713087448576E-4 42-43 4.262536525878468E-4 8.119117192149463E-5 0.0 0.28954801686503023 2.8416910172523123E-4 44-45 4.4655144556822045E-4 8.119117192149463E-5 0.0 0.3692371521059772 2.8416910172523123E-4 46-47 4.4655144556822045E-4 8.119117192149463E-5 0.0 0.4606990072755409 2.8416910172523123E-4 48-49 4.4655144556822045E-4 8.119117192149463E-5 0.0 0.5560580386973364 4.0595585960747315E-4 50-51 4.4655144556822045E-4 8.119117192149463E-5 0.0 0.6593129115884971 4.4655144556822045E-4 52-53 4.4655144556822045E-4 8.119117192149463E-5 0.0 0.7581225678169561 4.4655144556822045E-4 54-55 4.871470315289678E-4 8.119117192149463E-5 0.0 0.8631839442833702 4.4655144556822045E-4 56-57 6.089337894112098E-4 8.119117192149463E-5 0.0 0.9883401358003541 4.4655144556822045E-4 58-59 6.089337894112098E-4 8.119117192149463E-5 0.0 1.1179618417730204 4.4655144556822045E-4 60-61 6.089337894112098E-4 8.119117192149463E-5 0.0 1.2558244516957182 4.6684923854859416E-4 62-63 6.089337894112098E-4 8.119117192149463E-5 0.0 1.3903988191555956 5.277426174897151E-4 64-65 6.495293753719571E-4 8.119117192149463E-5 0.0 1.5271856460503337 5.277426174897151E-4 66-67 7.307205472934517E-4 8.119117192149463E-5 0.0 1.6894665009284209 5.277426174897151E-4 68-69 7.71316133254199E-4 8.119117192149463E-5 0.0 1.8671330828856316 5.277426174897151E-4 70-71 7.71316133254199E-4 8.119117192149463E-5 0.0 2.049995899845818 5.277426174897151E-4 72-73 7.71316133254199E-4 8.119117192149463E-5 0.0 2.239374308352704 5.683382034504625E-4 74-75 7.71316133254199E-4 8.119117192149463E-5 0.0 2.425931323635319 6.495293753719571E-4 76-77 7.71316133254199E-4 8.119117192149463E-5 0.0 2.6262705403516065 6.495293753719571E-4 78-79 7.71316133254199E-4 8.119117192149463E-5 0.0 2.847455590458738 6.495293753719571E-4 80-81 8.119117192149463E-4 8.119117192149463E-5 0.0 3.0838233897151897 6.495293753719571E-4 82-83 8.119117192149463E-4 8.119117192149463E-5 0.0 3.3273563098937124 6.901249613327044E-4 84-85 8.728050981560673E-4 8.119117192149463E-5 0.0 3.570889230072236 6.901249613327044E-4 86-87 9.336984770971883E-4 1.2178675788224195E-4 0.0 3.821932333653497 7.510183402738254E-4 88-89 9.336984770971883E-4 1.2178675788224195E-4 0.0 4.0921771493941925 8.119117192149463E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 44.46548 1 GTATCAA 9970 0.0 39.115875 1 TATCAAC 12365 0.0 31.555395 2 ATCAACG 12405 0.0 31.22568 3 TCAACGC 12460 0.0 30.973412 4 CAACGCA 12595 0.0 30.716892 5 AACGCAG 13150 0.0 29.416292 6 ACGCAGA 14840 0.0 25.931385 7 CGCAGAG 15100 0.0 25.452904 8 GTAAGGT 1430 0.0 23.265535 4 GTACATG 8550 0.0 23.169044 1 GTGTAAG 930 0.0 23.097006 1 GCAGAGT 16140 0.0 23.047039 9 TACATGG 8470 0.0 23.005148 2 GAGTACT 9505 0.0 22.66615 12-13 AGGTAAG 1390 0.0 22.565956 2 TAAGGTG 1540 0.0 22.529587 5 GTACTTT 9810 0.0 21.791946 14-15 AGAGTAC 13330 0.0 21.596418 10-11 ACATGGG 8760 0.0 21.105545 3 >>END_MODULE