FastQCFastQC Report
Thu 26 May 2016
SRR938186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463322
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.44289784283175326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91900.3730734349792678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57620.23391176630582602No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26090.10591388377158975No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24910.10112360462822156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.045.2873341
GTATCAA101650.038.225961
TATCAAC127550.030.0920772
TCAACGC127200.030.0591834
ATCAACG127700.029.9061133
CAACGCA128750.029.6604125
AACGCAG133800.028.6119386
CGCAGAG151900.024.889938
ACGCAGA152500.024.8231497
GCAGAGT165750.022.465819
GAGTACT95400.022.10518512-13
TACATGG85750.021.9372562
GTACATG88800.021.6647911
GTACTTT96250.021.61388814-15
AGAGTAC136450.021.28543310-11
CAGAGTA156450.020.44662510-11
ACATGGG88450.020.2459563
TAAGGTG15550.020.15965
GTATAGG4750.020.0008681
AGTACTT97850.019.80427212-13
Produced by FastQC (version 0.11.5)