##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2463322 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.477437785234734 33.0 31.0 34.0 31.0 34.0 2 32.70930231614056 34.0 31.0 34.0 31.0 34.0 3 32.673324884038706 34.0 31.0 34.0 31.0 34.0 4 36.20383084306477 37.0 35.0 37.0 35.0 37.0 5 36.161527400802655 37.0 35.0 37.0 35.0 37.0 6 36.22300495022575 37.0 37.0 37.0 35.0 37.0 7 36.1839491548405 37.0 37.0 37.0 35.0 37.0 8 36.17651366731592 37.0 37.0 37.0 35.0 37.0 9 37.87818198351657 39.0 38.0 39.0 35.0 39.0 10-11 37.84621864295451 39.0 38.0 39.0 35.0 39.0 12-13 37.787438670218506 39.0 38.0 39.0 35.0 39.0 14-15 39.06376166006717 40.0 38.0 41.0 36.0 41.0 16-17 38.99521276552558 40.0 38.0 41.0 36.0 41.0 18-19 38.98546515640261 40.0 38.0 41.0 35.5 41.0 20-21 38.983917855643725 40.0 38.5 41.0 35.5 41.0 22-23 39.01047305224408 40.0 38.5 41.0 35.0 41.0 24-25 38.959074980859185 40.0 38.0 41.0 35.0 41.0 26-27 38.787286639749084 40.0 38.0 41.0 35.0 41.0 28-29 38.70643099034556 40.0 38.0 41.0 34.5 41.0 30-31 38.489592306649314 40.0 38.0 41.0 34.0 41.0 32-33 38.285945158611014 40.0 38.0 41.0 34.0 41.0 34-35 38.07807485176522 40.0 38.0 41.0 33.5 41.0 36-37 38.00477221410762 40.0 37.5 41.0 33.0 41.0 38-39 37.96542230370207 40.0 38.0 41.0 33.0 41.0 40-41 37.83910061291216 40.0 37.0 41.0 33.0 41.0 42-43 37.64650926675441 40.0 37.0 41.0 33.0 41.0 44-45 37.3876902816603 40.0 37.0 41.0 32.5 41.0 46-47 37.02193663678561 40.0 36.5 41.0 31.5 41.0 48-49 36.63407889833323 39.5 36.0 41.0 30.5 41.0 50-51 36.31364088819895 39.0 35.0 41.0 30.0 41.0 52-53 35.83192676393911 39.0 35.0 40.0 28.5 41.0 54-55 35.49738625319792 38.0 35.0 40.0 28.0 41.0 56-57 35.200164046762865 38.0 34.5 40.0 28.0 41.0 58-59 34.79164112527717 38.0 34.0 40.0 26.5 41.0 60-61 34.35177333698152 37.0 34.0 40.0 26.0 41.0 62-63 33.894764468469816 36.5 33.0 39.5 25.5 41.0 64-65 33.42805589362658 36.0 33.0 39.0 24.5 40.5 66-67 32.891776836321036 35.5 32.0 39.0 22.5 40.0 68-69 32.339210018016324 35.0 31.5 38.0 21.5 40.0 70-71 31.725950159987203 35.0 31.0 37.0 19.5 39.5 72-73 31.24910060479304 35.0 30.5 37.0 18.0 39.0 74-75 30.691211096235083 34.0 30.0 36.0 15.5 39.0 76-77 29.512830234942896 33.0 28.5 35.0 14.0 37.0 78-79 30.20738985808595 34.0 30.0 35.0 13.5 37.0 80-81 30.026169132577877 34.0 30.0 35.0 8.5 37.0 82-83 29.575903799828037 34.0 29.5 35.0 6.5 36.5 84-85 29.073024760871704 34.0 29.0 35.0 2.0 36.0 86-87 28.538245710467407 34.0 28.5 35.0 2.0 35.5 88-89 27.899576060295814 33.0 27.0 35.0 2.0 35.0 90-91 27.35443478359711 33.0 26.0 35.0 2.0 35.0 92-93 26.708460160709805 32.5 25.0 35.0 2.0 35.0 94-95 25.932203341666252 32.0 23.5 34.0 2.0 35.0 96-97 25.079062339393715 32.0 19.5 34.0 2.0 35.0 98-99 24.02628726573302 31.0 6.0 34.0 2.0 35.0 100-101 20.521825404880076 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.060601307020398565 1101 2 0.043455243303654356 1101 3 0.2328803924846241 1101 4 0.07873480768589758 1101 5 -0.012874252037370582 1101 6 0.04992488037969878 1101 7 0.05130886661621048 1101 8 0.12899701971016242 1101 9 0.08422486387701866 1101 10-11 0.14176232821495205 1101 12-13 0.17723543965124833 1101 14-15 0.21125971632695695 1101 16-17 0.2525222409623282 1101 18-19 0.20293984123410524 1101 20-21 0.26826056194062886 1101 22-23 0.1233982950982977 1101 24-25 0.17101670652875356 1101 26-27 0.20270936344520152 1101 28-29 0.10756424605502701 1101 30-31 0.17473169930384103 1101 32-33 0.19842176646154286 1101 34-35 0.1716885153498353 1101 36-37 0.26510364653797325 1101 38-39 0.3330609890860643 1101 40-41 0.3225543027531188 1101 42-43 0.3928328084188948 1101 44-45 0.38738298651413317 1101 46-47 0.32333135343133534 1101 48-49 0.34828013782646394 1101 50-51 0.3908596877427044 1101 52-53 0.39683023821946506 1101 54-55 0.3807874832549629 1101 56-57 0.38784873778642037 1101 58-59 0.40029374910997007 1101 60-61 0.5053995361971886 1101 62-63 0.6094702535736118 1101 64-65 0.4358771133226753 1101 66-67 0.3843032822917891 1101 68-69 0.6300329101393132 1101 70-71 0.7602802818770478 1101 72-73 0.6878558571130284 1101 74-75 0.6718358358487535 1101 76-77 0.7296693989640239 1101 78-79 0.8667591930917773 1101 80-81 0.9175362518366974 1101 82-83 0.9760181458280179 1101 84-85 1.1162721717964175 1101 86-87 1.204452559124217 1101 88-89 1.4544021929898783 1101 90-91 1.4925169964197131 1101 92-93 1.6643686435895866 1101 94-95 1.881496585097544 1101 96-97 2.023092197719752 1101 98-99 2.368867016641449 1101 100-101 2.6063685893929254 1102 1 0.0435646106972456 1102 2 0.06126187893747215 1102 3 0.16279670104226796 1102 4 0.03793567067670267 1102 5 -0.00921846176828467 1102 6 -0.00743569629641172 1102 7 0.038933175445201584 1102 8 -0.01372506376482363 1102 9 0.0127457922273706 1102 10-11 0.07459546568137654 1102 12-13 0.12675794787707417 1102 14-15 0.1722582807538373 1102 16-17 0.1649270866980146 1102 18-19 0.2480464818479149 1102 20-21 0.25852230993618264 1102 22-23 0.2068309343294814 1102 24-25 0.10913952508305869 1102 26-27 0.20520779396107258 1102 28-29 0.17641950491501746 1102 30-31 0.22611166896113133 1102 32-33 0.20622585719792852 1102 34-35 0.10026826259328203 1102 36-37 0.11378194679743814 1102 38-39 0.13716505774574728 1102 40-41 0.1583458736920349 1102 42-43 0.12235363834564339 1102 44-45 0.25399604956288613 1102 46-47 0.2639507399032155 1102 48-49 0.3776193938302086 1102 50-51 0.3708758707808286 1102 52-53 0.33804810692863185 1102 54-55 0.33042115918874515 1102 56-57 0.4599830519051835 1102 58-59 0.5515581984612155 1102 60-61 0.5923734649188859 1102 62-63 0.46840666682103205 1102 64-65 0.5006503410016023 1102 66-67 0.5144131246140233 1102 68-69 0.5927341834899167 1102 70-71 0.7395212319041242 1102 72-73 0.608337909312052 1102 74-75 0.7216781816089508 1102 76-77 0.9062861636817807 1102 78-79 0.9838630017719545 1102 80-81 1.0857876955464292 1102 82-83 1.0080484642225294 1102 84-85 1.1197427805837243 1102 86-87 1.1820423647905187 1102 88-89 1.4191814398021911 1102 90-91 1.417601234877779 1102 92-93 1.5608743503946592 1102 94-95 1.4553681152239903 1102 96-97 1.6827649058709895 1102 98-99 1.987220726799979 1102 100-101 2.332827993965349 1103 1 0.27382226251751973 1103 2 0.29684784909328243 1103 3 0.3714354693303932 1103 4 0.20770236486697513 1103 5 0.05877232880415306 1103 6 0.08663897072165838 1103 7 0.1693055946395745 1103 8 0.13935970661398045 1103 9 0.28728584655495837 1103 10-11 0.2962582982018489 1103 12-13 0.2608642787276736 1103 14-15 0.4251787506084739 1103 16-17 0.41930668165703366 1103 18-19 0.45581923777425715 1103 20-21 0.3858240242972002 1103 22-23 0.3621191121217109 1103 24-25 0.498408518592818 1103 26-27 0.45381572552682314 1103 28-29 0.44894832643259264 1103 30-31 0.507218487199232 1103 32-33 0.6442542789455956 1103 34-35 0.6572729743173582 1103 36-37 0.6160916635659461 1103 38-39 0.5423632814682975 1103 40-41 0.6470388807794762 1103 42-43 0.7494125935419689 1103 44-45 0.7095599073728422 1103 46-47 0.7045780165005411 1103 48-49 0.6784043073411183 1103 50-51 0.7229010756775125 1103 52-53 0.8682218561348165 1103 54-55 0.884392082295065 1103 56-57 0.8538884183546998 1103 58-59 1.0156616180710145 1103 60-61 1.120949419565214 1103 62-63 0.9918075755206885 1103 64-65 0.9285451447405109 1103 66-67 0.8661817851768916 1103 68-69 1.025892118069713 1103 70-71 1.1545834450733032 1103 72-73 1.1072720119980097 1103 74-75 0.9929071084078309 1103 76-77 0.7857298363125231 1103 78-79 1.071743104445762 1103 80-81 1.3200828832139813 1103 82-83 1.4124651389500151 1103 84-85 1.5604729786555787 1103 86-87 1.7348068519518485 1103 88-89 2.021676859154045 1103 90-91 2.046965000257007 1103 92-93 2.1785165163765043 1103 94-95 2.3490716107223157 1103 96-97 2.5990892797789833 1103 98-99 2.68184114632707 1103 100-101 2.890338901772477 1104 1 0.1347294139925026 1104 2 0.11750260267609747 1104 3 0.20467775439174574 1104 4 0.14955660823425632 1104 5 0.06556642853092143 1104 6 0.10309626951360684 1104 7 0.12535948486038961 1104 8 0.054683519146017545 1104 9 0.10617227872269552 1104 10-11 0.11303012346511565 1104 12-13 0.09899210187136731 1104 14-15 0.19161922057289615 1104 16-17 0.12187678882646225 1104 18-19 0.12846013821145874 1104 20-21 0.15225866889824857 1104 22-23 0.18107199658275164 1104 24-25 0.10263743034589368 1104 26-27 0.143543279754482 1104 28-29 0.08245119941550882 1104 30-31 0.11908121402162664 1104 32-33 0.20652223530456126 1104 34-35 0.19247305613135524 1104 36-37 0.2030923867740242 1104 38-39 0.2585336703264005 1104 40-41 0.28654576161240186 1104 42-43 0.30434339685525913 1104 44-45 0.2867492083810177 1104 46-47 0.3831799897021355 1104 48-49 0.2429836384340618 1104 50-51 0.17037981525874102 1104 52-53 0.19990639587933146 1104 54-55 0.29145855012266253 1104 56-57 0.34876943422903395 1104 58-59 0.36013865578312476 1104 60-61 0.3660710013714379 1104 62-63 0.24345550609272237 1104 64-65 0.3715728751962004 1104 66-67 0.49540021127590705 1104 68-69 0.40866897817296177 1104 70-71 0.28769191938043903 1104 72-73 0.4527941630501111 1104 74-75 0.42331192369634607 1104 76-77 0.4380311569008235 1104 78-79 0.5374543683683832 1104 80-81 0.7630353154542178 1104 82-83 0.7221869800064837 1104 84-85 0.9402996158415924 1104 86-87 1.0096690713203635 1104 88-89 1.214629075640083 1104 90-91 1.2935893100593212 1104 92-93 1.5277802767478086 1104 94-95 1.486980831711577 1104 96-97 1.6851879346810037 1104 98-99 2.0404285086543155 1104 100-101 2.5109098995752355 1105 1 0.15806349542763343 1105 2 0.10995485061573618 1105 3 0.17753600482817689 1105 4 0.06463706530745839 1105 5 0.08451200029959693 1105 6 0.08889625599677231 1105 7 0.10730398420813714 1105 8 0.06552536319994573 1105 9 0.03432326436806932 1105 10-11 0.03434952637785926 1105 12-13 0.0738252772957182 1105 14-15 0.17789038367141785 1105 16-17 0.23068112875201763 1105 18-19 0.19781714471611878 1105 20-21 0.19961558486559028 1105 22-23 0.20733419521142338 1105 24-25 0.1815902769794988 1105 26-27 0.13839907038119037 1105 28-29 0.1249168685564328 1105 30-31 0.08755111418829387 1105 32-33 0.09700650795196708 1105 34-35 0.05208234934092815 1105 36-37 0.21850509030765863 1105 38-39 0.232076210752588 1105 40-41 0.248469877311436 1105 42-43 0.23147727222234948 1105 44-45 0.34339895136979237 1105 46-47 0.3005689890352201 1105 48-49 0.46455794853330445 1105 50-51 0.4073139091905702 1105 52-53 0.43546218663356484 1105 54-55 0.447403982897427 1105 56-57 0.4493885947312961 1105 58-59 0.4869830348820088 1105 60-61 0.4913327307362678 1105 62-63 0.5286273421003003 1105 64-65 0.6183427012752816 1105 66-67 0.693414415433736 1105 68-69 0.6728198975093136 1105 70-71 0.83159725942458 1105 72-73 0.7471686075526485 1105 74-75 0.7183046679590248 1105 76-77 0.7944198399908728 1105 78-79 0.9507615372663913 1105 80-81 1.0200510013636759 1105 82-83 0.9912895575147154 1105 84-85 1.1683532419887719 1105 86-87 1.3757682007594028 1105 88-89 1.4645823724099394 1105 90-91 1.568855598169474 1105 92-93 1.755817644432618 1105 94-95 1.8253988131884036 1105 96-97 1.9467484984097148 1105 98-99 2.287294450937413 1105 100-101 2.650137648116118 1106 1 0.015655942319845906 1106 2 -0.01907740744878339 1106 3 0.10475866415153945 1106 4 -0.005386538154382947 1106 5 0.02747030085034652 1106 6 0.00195683821549153 1106 7 0.05026228475888672 1106 8 0.032087125591765187 1106 9 0.011113193557683587 1106 10-11 -0.012857397304280482 1106 12-13 -0.022358829706639938 1106 14-15 -0.06011118989270159 1106 16-17 -0.059444756377800445 1106 18-19 -0.13754870894241122 1106 20-21 -0.14184782976855814 1106 22-23 -0.050730320917601546 1106 24-25 -0.1110926498497733 1106 26-27 -0.1159282309563352 1106 28-29 -0.17110988203365451 1106 30-31 -0.16048193065828542 1106 32-33 -0.07372519936511424 1106 34-35 -0.105274062932871 1106 36-37 -0.01182535815811292 1106 38-39 0.042658429477995696 1106 40-41 0.13458317392827723 1106 42-43 0.11700048229316451 1106 44-45 0.20453191753816213 1106 46-47 -0.10364029499309879 1106 48-49 0.04815354255377002 1106 50-51 0.11836819715280456 1106 52-53 0.06626470433615594 1106 54-55 0.2381199545732784 1106 56-57 0.4059188858406628 1106 58-59 0.32667618987807856 1106 60-61 0.2757544459211587 1106 62-63 0.29790350260384457 1106 64-65 0.36834270127528157 1106 66-67 0.47370552479644346 1106 68-69 0.20802839475557988 1106 70-71 0.33317082354732364 1106 72-73 0.3632189616045771 1106 74-75 0.4659443217749164 1106 76-77 0.47931362441258685 1106 78-79 0.7312493421444373 1106 80-81 0.810373582008836 1106 82-83 0.6541534442180996 1106 84-85 0.7499818808558025 1106 86-87 0.8826525437963824 1106 88-89 1.1870450002620245 1106 90-91 1.068855598169474 1106 92-93 1.1415375500187714 1106 94-95 1.4347615197186947 1106 96-97 1.3340419681186013 1106 98-99 1.61321891985952 1106 100-101 1.9331824946936145 1107 1 0.06733114620678293 1107 2 0.030863156460689822 1107 3 0.13825329330663294 1107 4 0.07663541675457708 1107 5 0.04788884011131245 1107 6 -0.010730209995976736 1107 7 -0.03325839549114562 1107 8 -0.03285913896317538 1107 9 -0.04489381099233469 1107 10-11 0.016452334166977778 1107 12-13 0.009500594565658105 1107 14-15 0.1337563493631606 1107 16-17 0.09792545382769191 1107 18-19 0.08562623713316952 1107 20-21 0.037185658111610564 1107 22-23 0.050316357185344884 1107 24-25 0.02404324327985563 1107 26-27 0.08240083951600496 1107 28-29 0.10805834564902028 1107 30-31 0.029009114634028776 1107 32-33 0.07005382315941233 1107 34-35 0.12639778445720395 1107 36-37 0.20815034248435182 1107 38-39 0.22525733290238037 1107 40-41 0.33986422729837784 1107 42-43 0.34443838935089843 1107 44-45 0.3094309181779735 1107 46-47 0.3453928449392194 1107 48-49 0.5152013070938608 1107 50-51 0.51209397765831 1107 52-53 0.3724462827175543 1107 54-55 0.4006849360825484 1107 56-57 0.4861557333372275 1107 58-59 0.645653250216732 1107 60-61 0.5709986888091478 1107 62-63 0.5332467246548518 1107 64-65 0.5048727854156709 1107 66-67 0.5997503806725462 1107 68-69 0.7612830868772278 1107 70-71 0.8966508328939149 1107 72-73 0.6243069495151907 1107 74-75 0.6551441107265532 1107 76-77 0.830854028506014 1107 78-79 1.0186532103046027 1107 80-81 1.0795583312149333 1107 82-83 1.1166631140185963 1107 84-85 1.323265320668174 1107 86-87 1.454598634077449 1107 88-89 1.3833913679415097 1107 90-91 1.4635266119845056 1107 92-93 1.6892057295586547 1107 94-95 1.8488862674190152 1107 96-97 1.8265078474832883 1107 98-99 1.8620076264324048 1107 100-101 2.163976973601226 1108 1 0.10707036697148453 1108 2 0.053885006495093535 1108 3 0.20328884698274408 1108 4 0.10027176068980737 1108 5 0.03016489700517866 1108 6 0.062384584023099876 1108 7 0.04999512016961205 1108 8 0.024641236719290305 1108 9 0.02651693868737226 1108 10-11 0.0577012709214344 1108 12-13 0.046991566067681845 1108 14-15 0.1150756634910195 1108 16-17 0.07400645695548036 1108 18-19 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-0.8936450666029856 2316 58-59 -1.0086211037132742 2316 60-61 -1.1291808007540922 2316 62-63 -1.270714343028743 2316 64-65 -1.1800206206369026 2316 66-67 -1.1497722620182387 2316 68-69 -1.2503152824857438 2316 70-71 -1.4220543935088763 2316 72-73 -1.2404484214410694 2316 74-75 -1.2794123923843408 2316 76-77 -1.31137373449328 2316 78-79 -1.6214460360776783 2316 80-81 -1.8752659313694977 2316 82-83 -2.0393930077154856 2316 84-85 -2.0535169494762755 2316 86-87 -2.029044024827545 2316 88-89 -2.1465268188964224 2316 90-91 -2.273433301789776 2316 92-93 -2.657512340146898 2316 94-95 -2.8188551620514595 2316 96-97 -2.965534424659811 2316 98-99 -3.7211593094440474 2316 100-101 -4.258346134939924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 13.0 5 38.0 6 69.0 7 238.0 8 566.0 9 1032.0 10 1779.0 11 2947.0 12 4458.0 13 6488.0 14 9110.0 15 12401.0 16 16347.0 17 22695.0 18 22888.0 19 17952.0 20 16379.0 21 16682.0 22 17736.0 23 19348.0 24 21791.0 25 24971.0 26 29080.0 27 35714.0 28 43063.0 29 51932.0 30 63496.0 31 78802.0 32 98688.0 33 128640.0 34 178594.0 35 264796.0 36 402262.0 37 530445.0 38 304190.0 39 17685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69363810334175 18.128446057803245 14.953099919539547 23.22481591931546 2 16.08237754004182 19.426970625850064 39.46383548852033 25.02681634558779 3 20.154996587704126 22.88828415310308 29.15330810007182 27.803411159120976 4 13.56249812245415 16.305541865821844 35.29672531646289 34.835234695261114 5 14.466736464265809 36.52369796286546 33.502644609109765 15.506920963758969 6 33.7098849154332 34.90217872715633 16.413789687815505 14.974146669594965 7 29.777471235997567 32.16039153630747 19.25054864934426 18.811588578350698 8 27.323331639140086 34.39846938029875 18.617992981818006 19.66020599874316 9 26.462882237888508 16.705124218433482 19.43424367581664 37.39774986786137 10-11 25.791248565960927 25.97624265118405 25.991141231231644 22.241367551623377 12-13 26.679865644848704 23.07095864852423 26.881503920315737 23.36767178631133 14-15 23.2833141586849 24.593455504396097 25.164229443004203 26.9590008939148 16-17 22.842125966910938 27.776317464041945 26.108630156477748 23.27292641256937 18-19 22.93469956424698 26.019639332576087 28.503439663998453 22.542221439178476 20-21 23.402624585823535 25.120203530029773 28.90257546516452 22.57459641898217 22-23 23.14419308559742 25.059858191499124 28.837094785009835 22.958853937893625 24-25 23.063840169497272 25.25759813777093 28.815287068952046 22.863274623779752 26-27 22.57726910425538 25.635712194589143 28.766167138151093 23.02085156300438 28-29 22.66863041526138 26.04962172940865 28.58804960467922 22.693698250650748 30-31 22.778801463552657 25.523612229181662 28.487761034798144 23.209825272467537 32-33 22.605077394252753 25.476244218977346 28.579951056173353 23.33872733059655 34-35 23.217161954157536 25.640978792186324 28.355061668844982 22.786797584811154 36-37 23.321881714056502 25.26921184385957 28.360560363377118 23.048346078706807 38-39 22.840870424614604 25.608433984435592 28.34126063120077 23.209434959749036 40-41 23.285616775157074 25.491394330779126 28.305945783086056 22.917043110977744 42-43 22.947857930859186 25.501303582181308 28.124526712979257 23.42631177398025 44-45 22.991965120001495 25.691904849774073 28.316653969116228 22.999476061108204 46-47 23.429998219845615 25.98785881934559 27.631791811655198 22.950351149153594 48-49 23.20894955866419 26.088887463529865 27.438551351693675 23.263611626112272 50-51 23.21225158546061 26.27009380016092 27.53018078838252 22.987473825995952 52-53 23.55016924299787 26.05053257349222 27.308813058138565 23.090485125371348 54-55 23.163658669065594 26.159166361523177 27.42286635689528 23.254308612515945 56-57 23.174436797138174 26.419871214563095 27.270023975753066 23.13566801254566 58-59 23.212454563390413 26.04334715477717 27.248853377674536 23.495344904157882 60-61 22.91949651730468 26.26292867923885 27.521249759471157 23.296325043985316 62-63 22.92166838115358 26.2471572940931 27.660817384004204 23.17035694074912 64-65 22.71954295865502 26.388328444271597 27.456296821933957 23.435831775139427 66-67 22.74148398773929 26.329595214254937 27.34306669202544 23.585854105980335 68-69 23.04167669717158 26.03556421234927 27.524713607941152 23.39804548253799 70-71 23.02226424316431 26.14355735872127 27.1327500018268 23.701428396287618 72-73 22.965917569850795 26.265851561428022 27.103257308626315 23.66497356009486 74-75 22.950673927322534 26.724581682784464 26.806503575253256 23.518240814639743 76-77 23.08679092704892 26.27819261956009 27.10435338944726 23.53066306394373 78-79 23.090891081230954 26.422124269583918 27.08320308916171 23.403781560023415 80-81 23.254236861537734 26.51095817394485 26.77300278770002 23.461802176817397 82-83 23.163211537071902 26.45374445762463 26.935984904765732 23.447059100537736 84-85 23.076534279467516 26.60411701529281 26.805317356391534 23.514031348848142 86-87 23.2744233666368 26.52381534254561 26.703756373038356 23.49800491777923 88-89 23.117688860345677 26.650439254478155 26.444010657967304 23.78786122720887 90-91 23.1028318983576 26.541942592309915 26.615362630438806 23.73986287889368 92-93 23.158547261577073 26.31284413855929 26.737842002203138 23.7907665976605 94-95 23.060944007037662 26.13565805639831 26.85302367517639 23.950374261387637 96-97 23.17180452211388 25.836077938811776 26.92176849345821 24.070349045616133 98-99 22.98313148832824 26.155215476689463 26.568195127099937 24.293457907882356 100-101 23.257571882441873 25.63886933484028 26.7416364830643 24.36192229965355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 553.0 2 444.0 3 595.5 4 492.5 5 467.0 6 944.5 7 2180.5 8 3626.5 9 4291.0 10 3608.0 11 2719.0 12 2629.5 13 2708.0 14 2848.0 15 2981.0 16 3086.0 17 3268.5 18 3513.5 19 3885.5 20 4257.0 21 4776.0 22 5498.5 23 6522.0 24 7905.0 25 9751.0 26 12331.0 27 15107.0 28 17449.0 29 19763.5 30 22555.5 31 26609.0 32 32458.5 33 35048.5 34 35114.0 35 37368.0 36 41230.0 37 45078.5 38 49153.5 39 53176.5 40 57451.0 41 62713.5 42 66536.0 43 69743.0 44 73969.5 45 79899.5 46 84993.0 47 90816.5 48 96664.5 49 97132.0 50 95516.5 51 94750.5 52 96066.5 53 99969.0 54 102420.5 55 97787.5 56 90095.0 57 82916.0 58 77042.5 59 69427.0 60 57529.5 61 47742.5 62 39295.0 63 29218.0 64 20517.0 65 15032.0 66 11061.0 67 8452.5 68 6355.0 69 4626.5 70 3407.5 71 2341.0 72 1653.5 73 1241.5 74 900.0 75 612.0 76 394.0 77 251.0 78 160.0 79 94.5 80 59.5 81 32.0 82 16.0 83 8.5 84 4.5 85 2.5 86 2.0 87 2.0 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01104199938132327 3 0.006941845199287791 4 0.0 5 3.6536027364672584E-4 6 1.2178675788224194E-4 7 0.0 8 2.4357351576448388E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.029779298037366E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015223344735280245 26-27 0.02163744731707832 28-29 2.4357351576448388E-4 30-31 4.059558596074732E-5 32-33 0.0036536027364672584 34-35 0.016603594657945652 36-37 0.008362690707913947 38-39 0.021272087043431594 40-41 0.02374841778703718 42-43 0.020013623878648426 44-45 0.010088003111245707 46-47 0.002131268262939234 48-49 2.0297792980373657E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.6387130874485753E-4 68-69 1.2178675788224194E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.059558596074732E-5 82-83 0.001136676406900925 84-85 0.004282834318858842 86-87 6.089337894112097E-4 88-89 2.029779298037366E-5 90-91 0.003978367424153237 92-93 6.292315823915834E-4 94-95 1.4208455086261562E-4 96-97 2.0297792980373657E-4 98-99 1.8268013682336292E-4 100-101 0.0025981175014878283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2463322.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.52476163272499 #Duplication Level Percentage of deduplicated Percentage of total 1 74.27431897305166 38.269665805194705 2 13.996910752344263 14.423749802181272 3 4.789715470982083 7.4036684379278075 4 2.234667644499253 4.605628708447481 5 1.186571878782767 3.056891660718836 6 0.7688238764369286 2.3768080182576212 7 0.4990911199182722 1.8000885690759119 8 0.3585367851576134 1.4778817913447646 9 0.26701985719773225 1.2382321043985678 >10 1.474636335301103 13.858678015539738 >50 0.10123200605244255 3.5549039288512234 >100 0.04278920882613271 3.9636323758477072 >500 0.003158939694350958 1.1077691620807333 >1k 0.002290231278402451 1.8100534688805765 >5k 1.579469847174104E-4 0.6084103919857557 >10k+ 7.89734923587052E-5 0.4439377592672951 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10910 0.44289784283175326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9190 0.3730734349792678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5762 0.23391176630582602 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2609 0.10591388377158975 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2491 0.10112360462822156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.059558596074732E-5 2 0.0 0.0 0.0 0.0 4.059558596074732E-5 3 0.0 0.0 0.0 0.0 4.059558596074732E-5 4 0.0 0.0 0.0 0.0 4.059558596074732E-5 5 0.0 0.0 0.0 0.0 4.059558596074732E-5 6 0.0 0.0 0.0 0.0 4.059558596074732E-5 7 4.059558596074732E-5 0.0 0.0 0.0 4.059558596074732E-5 8 4.059558596074732E-5 0.0 0.0 0.0 4.059558596074732E-5 9 4.059558596074732E-5 0.0 0.0 0.0 4.059558596074732E-5 10-11 4.059558596074732E-5 0.0 0.0 0.0 4.059558596074732E-5 12-13 4.059558596074732E-5 0.0 0.0 0.0 1.4208455086261562E-4 14-15 4.059558596074732E-5 0.0 0.0 0.0 1.6238234384298927E-4 16-17 4.059558596074732E-5 0.0 0.0 0.0 1.6238234384298927E-4 18-19 4.059558596074732E-5 0.0 0.0 0.0 1.6238234384298927E-4 20-21 4.059558596074732E-5 0.0 0.0 0.0 2.638713087448576E-4 22-23 4.059558596074732E-5 0.0 0.0 4.059558596074732E-5 3.2476468768597854E-4 24-25 4.059558596074732E-5 0.0 0.0 3.044668947056049E-4 3.450624806663522E-4 26-27 4.059558596074732E-5 0.0 0.0 7.10422754313078E-4 4.0595585960747315E-4 28-29 1.0148896490186829E-4 0.0 0.0 0.0030446689470560485 4.0595585960747315E-4 30-31 1.6238234384298927E-4 0.0 0.0 0.011691528756695227 4.0595585960747315E-4 32-33 1.6238234384298927E-4 0.0 0.0 0.02928971527067919 4.0595585960747315E-4 34-35 2.0297792980373657E-4 0.0 0.0 0.055108507941714485 4.4655144556822045E-4 36-37 2.8416910172523123E-4 0.0 0.0 0.09580558286736367 4.4655144556822045E-4 38-39 3.2476468768597854E-4 0.0 0.0 0.15568407215946595 4.4655144556822045E-4 40-41 3.2476468768597854E-4 0.0 0.0 0.2230727448543065 4.4655144556822045E-4 42-43 3.2476468768597854E-4 0.0 0.0 0.29005546168953955 4.4655144556822045E-4 44-45 3.2476468768597854E-4 0.0 0.0 0.3700287660322118 4.4655144556822045E-4 46-47 3.856580666270995E-4 0.0 0.0 0.46167330133859885 4.4655144556822045E-4 48-49 4.0595585960747315E-4 0.0 0.0 0.557722457721727 5.074448245093415E-4 50-51 4.0595585960747315E-4 0.0 0.0 0.6610788195777897 5.277426174897151E-4 52-53 4.0595585960747315E-4 0.0 0.0 0.7596652000834645 5.886359964308362E-4 54-55 4.0595585960747315E-4 0.0 0.0 0.8653558081322701 6.089337894112098E-4 56-57 4.871470315289678E-4 0.0 0.0 0.9910194444737636 6.089337894112098E-4 58-59 4.871470315289678E-4 0.0 0.0 1.1213312754077625 6.089337894112098E-4 60-61 4.871470315289678E-4 0.0 0.0 1.260046392635636 6.089337894112098E-4 62-63 4.871470315289678E-4 0.0 0.0 1.3947019512674348 6.495293753719571E-4 64-65 4.871470315289678E-4 0.0 0.0 1.5316714582989963 6.495293753719571E-4 66-67 4.871470315289678E-4 0.0 0.0 1.694459758001593 6.495293753719571E-4 68-69 4.871470315289678E-4 0.0 0.0 1.872085744372843 6.495293753719571E-4 70-71 5.074448245093415E-4 0.0 0.0 2.055861962017146 6.698271683523308E-4 72-73 5.277426174897151E-4 0.0 0.0 2.2475137233378337 7.10422754313078E-4 74-75 5.277426174897151E-4 0.0 0.0 2.4359178377816626 7.307205472934517E-4 76-77 5.277426174897151E-4 0.0 0.0 2.6387333852415558 7.71316133254199E-4 78-79 5.480404104700888E-4 0.0 0.0 2.864241865253507 7.71316133254199E-4 80-81 6.495293753719571E-4 0.0 0.0 3.105420241446307 7.71316133254199E-4 82-83 6.495293753719571E-4 0.0 0.0 3.3538652275260805 8.119117192149463E-4 84-85 7.307205472934517E-4 0.0 0.0 3.6023508091918153 8.119117192149463E-4 86-87 8.119117192149463E-4 0.0 0.0 3.859787717561894 8.525073051756936E-4 88-89 8.119117192149463E-4 0.0 0.0 4.137826885807052 8.931028911364409E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6220 0.0 45.287334 1 GTATCAA 10165 0.0 38.22596 1 TATCAAC 12755 0.0 30.092077 2 TCAACGC 12720 0.0 30.059183 4 ATCAACG 12770 0.0 29.906113 3 CAACGCA 12875 0.0 29.660412 5 AACGCAG 13380 0.0 28.611938 6 CGCAGAG 15190 0.0 24.88993 8 ACGCAGA 15250 0.0 24.823149 7 GCAGAGT 16575 0.0 22.46581 9 GAGTACT 9540 0.0 22.105185 12-13 TACATGG 8575 0.0 21.937256 2 GTACATG 8880 0.0 21.664791 1 GTACTTT 9625 0.0 21.613888 14-15 AGAGTAC 13645 0.0 21.285433 10-11 CAGAGTA 15645 0.0 20.446625 10-11 ACATGGG 8845 0.0 20.245956 3 TAAGGTG 1555 0.0 20.1596 5 GTATAGG 475 0.0 20.000868 1 AGTACTT 9785 0.0 19.804272 12-13 >>END_MODULE