FastQCFastQC Report
Thu 26 May 2016
SRR938185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474890
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111140.4490704637377823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88350.35698556299471895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.23039407812064377No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25540.10319650570328379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.043.4453771
GTATCAA103800.037.7118341
ATCAACG130400.029.847833
TATCAAC130650.029.827092
TCAACGC131300.029.679434
CAACGCA133600.029.061775
AACGCAG137850.028.26926
ACGCAGA152300.025.314937
CGCAGAG153300.025.0252488
GAGTACT94100.022.53001612-13
GCAGAGT167600.022.2092959
GTACTTT97200.021.77247614-15
AGAGTAC137050.021.55781610-11
GTACATG91500.021.28631
TACATGG89250.021.1924552
ACCGTAT2257.098788E-821.1282988
AGTACTT97400.020.35135712-13
CAGAGTA158150.020.06433310-11
TGGTATC36250.019.9270062
CCGTATG2151.0714284E-619.8999089
Produced by FastQC (version 0.11.5)