##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474890 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85163461810424 33.0 31.0 34.0 30.0 34.0 2 32.039926218943066 34.0 31.0 34.0 30.0 34.0 3 32.10986185244597 34.0 31.0 34.0 30.0 34.0 4 35.6270795873756 37.0 35.0 37.0 33.0 37.0 5 35.64402175450222 37.0 35.0 37.0 33.0 37.0 6 35.672355134975696 37.0 35.0 37.0 33.0 37.0 7 35.6114837427118 37.0 35.0 37.0 33.0 37.0 8 35.59041815999903 37.0 35.0 37.0 33.0 37.0 9 37.24869711381112 39.0 37.0 39.0 34.0 39.0 10-11 37.16955925313852 39.0 37.0 39.0 34.0 39.0 12-13 37.067638157655495 39.0 37.0 39.0 33.0 39.0 14-15 38.312395096347714 40.0 38.0 41.0 33.5 41.0 16-17 38.232319012158115 40.0 38.0 41.0 33.0 41.0 18-19 38.166032429724154 40.0 38.0 41.0 33.0 41.0 20-21 38.06102069182873 40.0 38.0 41.0 33.0 41.0 22-23 37.90829188367968 40.0 38.0 41.0 33.0 41.0 24-25 37.85220716880346 40.0 38.0 41.0 33.0 41.0 26-27 37.85905272557568 40.0 38.0 41.0 33.0 41.0 28-29 37.768107875501535 40.0 38.0 41.0 33.0 41.0 30-31 37.581405638230386 40.0 37.5 41.0 32.0 41.0 32-33 37.35690838784754 40.0 37.0 41.0 31.5 41.0 34-35 37.146887942494416 40.0 36.5 41.0 31.0 41.0 36-37 36.92804225642352 40.0 36.0 41.0 30.5 41.0 38-39 36.684324353809664 39.5 36.0 41.0 30.0 41.0 40-41 36.45253142563912 39.0 35.5 41.0 30.0 41.0 42-43 36.15869978059631 39.0 35.0 41.0 29.5 41.0 44-45 35.93151432992981 39.0 35.0 40.5 29.0 41.0 46-47 35.7883804936785 39.0 35.0 41.0 27.5 41.0 48-49 35.520690616552656 39.0 35.0 41.0 26.5 41.0 50-51 34.85615946567322 38.5 34.5 40.0 25.5 40.5 52-53 34.92589205984912 38.5 34.5 40.0 26.0 41.0 54-55 35.05058103592483 39.0 35.0 40.0 26.0 41.0 56-57 34.82191673165272 38.0 34.5 40.0 25.5 41.0 58-59 34.53667173086481 38.0 34.0 40.0 25.0 41.0 60-61 34.142292586741235 37.5 34.0 40.0 23.5 41.0 62-63 33.70110166512451 37.0 33.5 40.0 22.5 41.0 64-65 33.258371685206214 36.0 33.0 39.0 21.5 41.0 66-67 32.814526706237444 36.0 32.5 39.0 20.0 41.0 68-69 32.31160192978274 35.0 32.0 39.0 18.0 40.0 70-71 31.817277939625598 35.0 31.5 38.0 14.5 40.0 72-73 31.334417893320513 35.0 31.0 37.0 10.0 39.5 74-75 30.81208740590491 35.0 31.0 36.5 7.0 39.0 76-77 30.28849363001992 34.5 30.0 36.0 4.5 39.0 78-79 29.740592511182314 34.0 29.5 35.5 2.0 37.5 80-81 29.229733038640102 34.0 29.0 35.0 2.0 37.0 82-83 28.713800613360593 34.0 29.0 35.0 2.0 36.5 84-85 28.231172900613764 34.0 27.5 35.0 2.0 36.0 86-87 27.753126199548262 33.5 26.5 35.0 2.0 35.5 88-89 27.2119570970831 33.0 25.5 35.0 2.0 35.0 90-91 26.611852647996475 33.0 25.0 35.0 2.0 35.0 92-93 26.00136511117666 32.0 23.5 35.0 2.0 35.0 94-95 25.395497173611755 32.0 21.0 34.0 2.0 35.0 96-97 24.5559085454303 32.0 12.5 34.0 2.0 35.0 98-99 23.482047484938725 31.0 2.0 34.0 2.0 35.0 100-101 20.61954793950438 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3686601425269309 1101 2 0.3094738760815048 1101 3 0.28911685295411615 1101 4 0.1450446128850018 1101 5 0.14745475208897574 1101 6 0.0717659936706525 1101 7 0.20240058325232013 1101 8 0.1605980914533447 1101 9 0.1431376718386943 1101 10-11 0.15528699881864583 1101 12-13 0.07122336653854688 1101 14-15 0.16713001285780393 1101 16-17 0.2991793546331323 1101 18-19 0.1612354107758236 1101 20-21 0.33108152604915375 1101 22-23 0.35333092544266975 1101 24-25 0.33810498755198637 1101 26-27 0.352232838458292 1101 28-29 0.2875655423734287 1101 30-31 0.40506673669005977 1101 32-33 0.4376209598318823 1101 34-35 0.340225757670062 1101 36-37 0.43689914924845397 1101 38-39 0.3623158492144114 1101 40-41 0.4786692417095324 1101 42-43 0.3784094181313833 1101 44-45 0.4419494544325673 1101 46-47 0.6081221213791039 1101 48-49 0.7167358013076779 1101 50-51 0.7759539546634571 1101 52-53 0.8681190773712899 1101 54-55 0.8923640290900536 1101 56-57 0.6776735289140703 1101 58-59 0.6683076486943804 1101 60-61 0.6500112447502104 1101 62-63 0.6081974818120344 1101 64-65 0.5587987426860579 1101 66-67 0.5017322804746627 1101 68-69 0.574901258700713 1101 70-71 0.5556592589804374 1101 72-73 0.535457594944166 1101 74-75 0.5906642440024399 1101 76-77 0.6738261265347703 1101 78-79 0.6352445359451124 1101 80-81 0.7756608972891996 1101 82-83 0.6740083608700473 1101 84-85 0.5288297736501875 1101 86-87 0.6835984854595161 1101 88-89 0.8693594121988681 1101 90-91 0.8935141137005118 1101 92-93 1.0643308278906183 1101 94-95 0.9097232908530763 1101 96-97 0.8176095837501904 1101 98-99 0.7812006169519563 1101 100-101 0.8135386272069773 1102 1 0.45354858139350185 1102 2 0.28855480899652974 1102 3 0.19273883978069506 1102 4 0.14570069828143062 1102 5 0.1839280430825312 1102 6 0.2284641293691081 1102 7 0.1352941308777318 1102 8 0.21384956398578225 1102 9 0.3785552185774108 1102 10-11 0.3595393485397267 1102 12-13 0.1772200364916685 1102 14-15 0.329725502655684 1102 16-17 0.2682296524856369 1102 18-19 0.36471608543921263 1102 20-21 0.4197395566978983 1102 22-23 0.4590286402274515 1102 24-25 0.3482349574796615 1102 26-27 0.3404972558921031 1102 28-29 0.3696277379762307 1102 30-31 0.3757210312071919 1102 32-33 0.46908587247613553 1102 34-35 0.30961293854849004 1102 36-37 0.31000518032914925 1102 38-39 0.3535399703432489 1102 40-41 0.390319671957819 1102 42-43 0.32620367081570123 1102 44-45 0.3082578438465333 1102 46-47 0.22155056016201513 1102 48-49 0.16483813465989527 1102 50-51 0.26778092910787876 1102 52-53 0.34810124842650225 1102 54-55 0.24991792752248188 1102 56-57 0.16917987512867683 1102 58-59 0.19866476371331032 1102 60-61 0.2559833899232089 1102 62-63 0.24731600798302367 1102 64-65 0.11999605385761214 1102 66-67 0.16187101468421616 1102 68-69 0.14246664765801853 1102 70-71 0.21215169070933726 1102 72-73 0.21898431915978378 1102 74-75 0.2979805752626348 1102 76-77 0.1080180616463835 1102 78-79 0.1916620815431429 1102 80-81 0.3338907100681716 1102 82-83 0.48254958132196535 1102 84-85 0.4681918746139502 1102 86-87 0.3879243144636213 1102 88-89 0.33119020414394384 1102 90-91 0.433440835938935 1102 92-93 0.5518064599550385 1102 94-95 0.6165073963620991 1102 96-97 0.7341875936536404 1102 98-99 0.8043060967003832 1102 100-101 0.7274956225297764 1103 1 0.36834815841890034 1103 2 0.3463072225990018 1103 3 0.34927637146356716 1103 4 0.21716395346001605 1103 5 0.13210524486861885 1103 6 0.15654638125545972 1103 7 0.26120293392689575 1103 8 0.24791020789587037 1103 9 0.2599746113485182 1103 10-11 0.23858444535439816 1103 12-13 0.15203242561074148 1103 14-15 0.3291454117132204 1103 16-17 0.3542017627807965 1103 18-19 0.29258626499866125 1103 20-21 0.3283755074912733 1103 22-23 0.3927785250502396 1103 24-25 0.370267444035008 1103 26-27 0.23661454066428433 1103 28-29 0.4724630718585132 1103 30-31 0.4489945796452446 1103 32-33 0.5210574869608209 1103 34-35 0.4733037063819481 1103 36-37 0.5273259316794565 1103 38-39 0.4614628462195185 1103 40-41 0.5704094809318434 1103 42-43 0.4831536466102051 1103 44-45 0.46591826193615304 1103 46-47 0.6519754799767412 1103 48-49 0.7304454513014278 1103 50-51 0.7152443978213583 1103 52-53 0.6991112977296581 1103 54-55 0.7659771044770949 1103 56-57 0.8045140133618105 1103 58-59 0.8289285451237873 1103 60-61 0.8423524045474338 1103 62-63 0.883778700073016 1103 64-65 0.6732592985057053 1103 66-67 0.7894974207603056 1103 68-69 0.8403789418564571 1103 70-71 1.014534652379833 1103 72-73 1.0514246939281442 1103 74-75 0.8777806495610818 1103 76-77 0.886261890707317 1103 78-79 0.9489628542176973 1103 80-81 0.9776219983417391 1103 82-83 0.9996599788625105 1103 84-85 1.1182037945412944 1103 86-87 1.152881080960288 1103 88-89 1.289761509441803 1103 90-91 1.6089350741532016 1103 92-93 1.5416463672867486 1103 94-95 1.4973509030730945 1103 96-97 1.2816100739053198 1103 98-99 1.5412190402789072 1103 100-101 1.118415855546079 1104 1 0.3864156024122316 1104 2 0.4005274431374062 1104 3 0.2933256049044388 1104 4 0.26560272952485064 1104 5 0.269335772656774 1104 6 0.23910672245906994 1104 7 0.2745821995740485 1104 8 0.2741948643977068 1104 9 0.31251158734276174 1104 10-11 0.3748872621831296 1104 12-13 0.20109386111251126 1104 14-15 0.3534324838584695 1104 16-17 0.4863208930115732 1104 18-19 0.45247970423226036 1104 20-21 0.38666245097650176 1104 22-23 0.3952014786851663 1104 24-25 0.5160103455454248 1104 26-27 0.48469986289570954 1104 28-29 0.5407278748126032 1104 30-31 0.38313114365222134 1104 32-33 0.5153014992819109 1104 34-35 0.580481370039891 1104 36-37 0.6125829730632617 1104 38-39 0.6204501611675397 1104 40-41 0.594380407208277 1104 42-43 0.6105889846317183 1104 44-45 0.6377505938542285 1104 46-47 0.7227890584934045 1104 48-49 0.8136362781862871 1104 50-51 0.8823759950012899 1104 52-53 0.9743632339941044 1104 54-55 0.9584314181309495 1104 56-57 0.9039129061976467 1104 58-59 0.8998963264540549 1104 60-61 0.9217893002495643 1104 62-63 0.9132932733999368 1104 64-65 0.8460377459577231 1104 66-67 1.0078944442417992 1104 68-69 1.075137632083731 1104 70-71 0.887575425220593 1104 72-73 0.8361290992608623 1104 74-75 0.806937572123573 1104 76-77 0.9186963411811213 1104 78-79 0.8746456983333566 1104 80-81 0.9813687913016302 1104 82-83 0.9966004333557379 1104 84-85 1.0144475756871643 1104 86-87 1.0590340054299787 1104 88-89 1.0068854351822694 1104 90-91 1.1959959358450583 1104 92-93 1.261192472637969 1104 94-95 1.2650232507330976 1104 96-97 1.2883058129195106 1104 98-99 1.4122542305134012 1104 100-101 1.0200979304537263 1105 1 0.33673912311173737 1105 2 0.20097890253942552 1105 3 0.26720247142375086 1105 4 0.12422429890845876 1105 5 0.11323378859593447 1105 6 0.11810807400990342 1105 7 0.12673187552732657 1105 8 0.15238498956163937 1105 9 0.16472422169648127 1105 10-11 0.23937546956513955 1105 12-13 0.1998549943111243 1105 14-15 0.24410801598845921 1105 16-17 0.3219372491766137 1105 18-19 0.29538550893103377 1105 20-21 0.31884992498356013 1105 22-23 0.2568849139848126 1105 24-25 0.3085611010297882 1105 26-27 0.3686629281774856 1105 28-29 0.3445005953157718 1105 30-31 0.3392502892196063 1105 32-33 0.3389775400105677 1105 34-35 0.34072978120328656 1105 36-37 0.41299719988643346 1105 38-39 0.3711930045398617 1105 40-41 0.41931298582984056 1105 42-43 0.3942647426229158 1105 44-45 0.2873424656124044 1105 46-47 0.24152682639948608 1105 48-49 0.13025103293947637 1105 50-51 0.09971307822243602 1105 52-53 0.0602643824013569 1105 54-55 0.06528711123061015 1105 56-57 0.3163865105183987 1105 58-59 0.1813246503699233 1105 60-61 0.15511559802273922 1105 62-63 0.2230769595140103 1105 64-65 0.17347756479534127 1105 66-67 0.3141458846412277 1105 68-69 0.3446669826982358 1105 70-71 0.24566217696493808 1105 72-73 0.26281839807523966 1105 74-75 0.27243480704980527 1105 76-77 0.3510955395217792 1105 78-79 0.33201119009435587 1105 80-81 0.5341922043307612 1105 82-83 0.4506112122284094 1105 84-85 0.4128099504319991 1105 86-87 0.5026596893012218 1105 88-89 0.5751264235009366 1105 90-91 0.4960487682991115 1105 92-93 0.5839985203265741 1105 94-95 0.7499194811522933 1105 96-97 0.805351840934847 1105 98-99 0.8879295210014924 1105 100-101 0.48986499423502394 1106 1 0.047369349101785474 1106 2 0.10952437471224385 1106 3 -8.102299046441885E-5 1106 4 -0.019053454764140554 1106 5 0.06614953542477764 1106 6 0.20094719814946416 1106 7 0.22496689945503334 1106 8 0.048135219620426994 1106 9 0.06704626585823803 1106 10-11 -0.3692888861828294 1106 12-13 -0.06896135122684655 1106 14-15 0.006160871498821052 1106 16-17 0.14753464708851993 1106 18-19 0.020549251983567274 1106 20-21 -0.02364610560830016 1106 22-23 -0.05052409160494875 1106 24-25 0.04886422235937715 1106 26-27 0.0502555230658146 1106 28-29 -0.03232719991839872 1106 30-31 0.1077652140352825 1106 32-33 0.03146323981047061 1106 34-35 -0.1672482126204855 1106 36-37 -0.19829364051555132 1106 38-39 -0.02393650385748458 1106 40-41 0.23818066745707966 1106 42-43 0.08547594056565799 1106 44-45 0.32972247346380357 1106 46-47 0.3343510583051028 1106 48-49 0.40500074963737376 1106 50-51 0.3771036632154505 1106 52-53 0.3733116163715451 1106 54-55 0.1349107684263231 1106 56-57 0.1319309353078495 1106 58-59 0.2697497131890856 1106 60-61 0.04823497270785282 1106 62-63 0.18897159815085018 1106 64-65 0.15776101311833202 1106 66-67 -0.05586772021710118 1106 68-69 -0.26414931515915185 1106 70-71 0.020817019642866086 1106 72-73 0.09721707446238881 1106 74-75 0.18318733372311513 1106 76-77 0.1416783869105558 1106 78-79 0.33044154545770255 1106 80-81 0.18101945918504114 1106 82-83 0.10820950971389465 1106 84-85 0.06371619928965444 1106 86-87 0.21290092124021598 1106 88-89 0.14677761781995713 1106 90-91 0.038248464097023316 1106 92-93 -0.006814306384722357 1106 94-95 -0.010238627645247078 1106 96-97 0.29037862947307147 1106 98-99 0.12712462515971268 1106 100-101 -0.04180579680166119 1107 1 0.2223630157503571 1107 2 0.18492970920367924 1107 3 0.20451472823548755 1107 4 0.07160168520292842 1107 5 0.20725570049000908 1107 6 0.13408826902871596 1107 7 0.15231792998178406 1107 8 0.12569434597983786 1107 9 0.24913780992931578 1107 10-11 0.23964453219836912 1107 12-13 0.19237760974161233 1107 14-15 0.17487682973489171 1107 16-17 0.2545087979670413 1107 18-19 0.40318274998404036 1107 20-21 0.4406261018361022 1107 22-23 0.5357474644090203 1107 24-25 0.5126539641395951 1107 26-27 0.47301487380681095 1107 28-29 0.506928947956311 1107 30-31 0.5420720739596447 1107 32-33 0.5361600984681658 1107 34-35 0.5526690466669137 1107 36-37 0.5900434928286487 1107 38-39 0.545695754615636 1107 40-41 0.45935079937174805 1107 42-43 0.5589986863449354 1107 44-45 0.49442489642311216 1107 46-47 0.5013499997306496 1107 48-49 0.39821184928813835 1107 50-51 0.3741765238998127 1107 52-53 0.33633049880643995 1107 54-55 0.3486554981731729 1107 56-57 0.3386607997741322 1107 58-59 0.3498240848301464 1107 60-61 0.23134980541690453 1107 62-63 0.2638618954564649 1107 64-65 0.4245179300428603 1107 66-67 0.43286796188484544 1107 68-69 0.39527493795775825 1107 70-71 0.4805364521745794 1107 72-73 0.5396048384274295 1107 74-75 0.3927504681892131 1107 76-77 0.47726820288145433 1107 78-79 0.35413777982789796 1107 80-81 0.3747146078378485 1107 82-83 0.41618998489620296 1107 84-85 0.5668896607376404 1107 86-87 0.6359493442357085 1107 88-89 0.6326836489800449 1107 90-91 0.6942219454498257 1107 92-93 0.7035479613169962 1107 94-95 0.7824723634337758 1107 96-97 0.7691107829935078 1107 98-99 0.6163185704757481 1107 100-101 0.9535373803693226 1108 1 0.29129000602603483 1108 2 0.2548354461878546 1108 3 0.1582517584400165 1108 4 0.09192240843094623 1108 5 0.16958206663392872 1108 6 0.22679847974399792 1108 7 0.18415714759816382 1108 8 0.14241743000274454 1108 9 0.2150880764954195 1108 10-11 0.27712157576366536 1108 12-13 0.12738360556106443 1108 14-15 0.14158793392169855 1108 16-17 0.3634448262323744 1108 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2316 64-65 -0.9556358862490768 2316 66-67 -1.2070363337213053 2316 68-69 -1.248791270785567 2316 70-71 -1.3066809108841575 2316 72-73 -1.2536194014039772 2316 74-75 -1.2026431636657726 2316 76-77 -1.1290904350738913 2316 78-79 -1.1170704881444067 2316 80-81 -0.9276253170563855 2316 82-83 -0.9169266667176181 2316 84-85 -1.1472770455417702 2316 86-87 -1.2254492233674021 2316 88-89 -1.0988294786839106 2316 90-91 -1.0345949406714539 2316 92-93 -1.1301569395719149 2316 94-95 -1.2694296425556715 2316 96-97 -1.3513971316330995 2316 98-99 -1.272213143488539 2316 100-101 -1.1669368634233628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7579.0 3 2002.0 4 2093.0 5 2389.0 6 2660.0 7 3288.0 8 3977.0 9 4943.0 10 6246.0 11 7539.0 12 9212.0 13 11317.0 14 12854.0 15 15991.0 16 19209.0 17 25341.0 18 22964.0 19 18701.0 20 18543.0 21 19310.0 22 20990.0 23 22720.0 24 25054.0 25 28257.0 26 31770.0 27 37269.0 28 43020.0 29 51253.0 30 60963.0 31 75572.0 32 94423.0 33 122331.0 34 168983.0 35 248530.0 36 372400.0 37 489438.0 38 330366.0 39 35386.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.447959108262566 17.748623663965294 14.491667268987102 23.31174995878504 2 15.833409467414478 19.117469901435335 40.029049531350935 25.020071099799246 3 19.91620019130678 22.894837061767188 28.994903606771583 28.194059140154454 4 13.328810396071027 15.947477862742332 35.58008489806612 35.143626843120515 5 14.53807144367762 36.529895845843605 33.39467660128372 15.537356109195061 6 33.732286751375845 34.70542838213131 16.54575371455429 15.016531151938558 7 29.782789768637947 32.17432617116425 19.348773643649068 18.694110416548735 8 27.23227081599181 34.20197599360606 18.8145493737647 19.75120381663743 9 26.675097014355174 16.75353724910586 19.474507429041765 37.0968583074972 10-11 25.805557465013123 25.872882692362126 26.095379427189684 22.226180415435067 12-13 26.742622886167805 23.122550947868497 26.794842365732535 23.339983800231163 14-15 23.420493505385682 24.59263499679307 25.175602913950172 26.811268583871072 16-17 22.93920743031302 27.748065393255654 25.98972158360305 23.323005592828274 18-19 22.98029094103737 26.052044982396094 28.419865075452105 22.547799001114434 20-21 23.513404229509522 25.138651894383568 28.807630099874654 22.54031377623226 22-23 23.20890694806639 25.04510077425354 28.74740736248408 22.998584915195988 24-25 23.081651548855312 25.254580384435034 28.73787456088789 22.92589350582177 26-27 22.65666789983443 25.577071974544186 28.67717487329707 23.089085252324317 28-29 22.710994298902012 26.05507510121617 28.48074562865618 22.753184971225636 30-31 22.83024063264069 25.5024787834654 28.451970310396817 23.215310273497092 32-33 22.602555697006345 25.508874099565315 28.523211306251202 23.36535889717714 34-35 23.292497570920432 25.681749535715255 28.223114648107945 22.80263824525637 36-37 23.408222853551308 25.29554550451619 28.258288485405224 23.03794315652727 38-39 22.90354350800158 25.63186701582934 28.206951845826772 23.25763763034231 40-41 23.281820123965595 25.518168880944398 28.25412725159333 22.94588374349668 42-43 23.02807429212988 25.485244347700426 28.04266168664033 23.44401967352936 44-45 23.05236637223077 25.736015642176323 28.196231231328323 23.015386754264576 46-47 23.46979337494329 26.053147169704555 27.483022067018503 22.994037388333652 48-49 23.305426841603126 26.14793209238465 27.298668171826712 23.247972894185512 50-51 23.251293347344596 26.348476275159648 27.411203621372564 22.98902675612319 52-53 23.51798708422695 26.09728195665656 27.288546501653123 23.096184457463366 54-55 23.163046050375474 26.21300463427838 27.351174351967916 23.27277496337823 56-57 23.24580255408408 26.415138187083276 27.20423882171426 23.134820437118385 58-59 23.208477957718117 26.06621606029334 27.26767364373025 23.457632338258293 60-61 22.99346356358551 26.21071595652345 27.54158654842005 23.254233931470985 62-63 22.97053527459868 26.201929343098264 27.70520361684642 23.12233176545664 64-65 22.781700970409208 26.350786904022407 27.506156244329244 23.361355881239145 66-67 22.832150593255957 26.360103941030033 27.37381187158514 23.433933594128867 68-69 23.066403226908037 26.002417513759664 27.628318777195517 23.30286048213679 70-71 23.08036513696274 26.107846612725304 27.218957274706835 23.59283097560512 72-73 23.012368803396434 26.217513979854136 27.22220010335462 23.54791711339481 74-75 22.96790595501054 26.69031803034192 26.98773510742138 23.35404090722616 76-77 23.225400479399212 26.256361779491723 27.087914508353112 23.430323232755956 78-79 23.234007472171314 26.408730599733687 27.07500257626242 23.282259351832582 80-81 23.38717711060555 26.511882224642942 26.793760369630142 23.307180295121366 82-83 23.288815028504594 26.51101021768247 26.903317170087515 23.296857583725426 84-85 23.201371469031013 26.64891710282825 26.859248626045545 23.290462802095192 86-87 23.4866239065406 26.508597654422882 26.730244488378695 23.27453395065782 88-89 23.347823969191918 26.606246461806304 26.501694796043406 23.544234772958365 90-91 23.352194274771435 26.59131992429751 26.58747941537752 23.46900638555354 92-93 23.43039097975631 26.32300215090097 26.75888143448907 23.487725434853647 94-95 23.407394830248784 26.13354112596114 26.913511371997316 23.54555267179276 96-97 23.546817265143623 25.80587526471757 26.910746472217745 23.736560997921067 98-99 23.362524531742068 26.143627324869485 26.559254804947514 23.934593338440934 100-101 23.88523773089159 25.404282246103993 26.779791968648585 23.930688054355834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 903.0 1 604.5 2 442.5 3 610.0 4 527.5 5 510.0 6 985.0 7 2190.5 8 3689.5 9 4284.0 10 3590.5 11 2783.5 12 2571.0 13 2679.5 14 2919.0 15 3036.0 16 3127.5 17 3289.0 18 3517.0 19 3776.5 20 4103.0 21 4751.0 22 5615.5 23 6754.0 24 8233.0 25 10057.0 26 12374.0 27 15130.5 28 17540.5 29 19863.0 30 22623.5 31 26561.5 32 32398.0 33 35110.5 34 35095.5 35 37435.0 36 41468.5 37 45581.5 38 49963.0 39 53597.5 40 57689.5 41 63520.0 42 67154.5 43 70115.0 44 74705.5 45 80592.5 46 85961.5 47 91589.0 48 96937.0 49 97656.0 50 96223.0 51 95162.0 52 95924.5 53 99381.0 54 101786.5 55 97109.5 56 89341.5 57 81909.0 58 75874.0 59 68631.0 60 57184.5 61 47794.0 62 39821.5 63 30143.0 64 21422.0 65 15866.5 66 11734.0 67 8927.5 68 6843.5 69 5024.0 70 3777.0 71 2662.5 72 1901.0 73 1430.0 74 1038.5 75 749.0 76 501.5 77 316.0 78 205.5 79 132.5 80 87.5 81 58.0 82 42.0 83 29.0 84 17.0 85 14.0 86 12.5 87 8.0 88 7.5 89 8.0 90 8.5 91 6.5 92 3.0 93 2.0 94 1.5 95 1.5 96 1.0 97 1.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49262795518184643 2 0.04784051008327643 3 0.055598430637321256 4 0.06347756870002304 5 0.07761961137666724 6 0.10457030413472922 7 0.06909397993446173 8 0.052487181248459525 9 0.052002311213831726 10-11 0.07353862191854992 12-13 0.10636836384647398 14-15 0.05416402345154733 16-17 0.05877028878051145 18-19 0.0579217662199128 20-21 0.0723264468319804 22-23 0.06222498777723454 24-25 0.08665031577161005 26-27 0.0640432504070888 28-29 0.06442710585116915 30-31 0.06873032740849089 32-33 0.06907377701635224 34-35 0.06994250249506039 36-37 0.06563928093773864 38-39 0.03925426988674244 40-41 0.042769577637794 42-43 0.04070887999062585 44-45 0.02238483326531684 46-47 0.029193216668215557 48-49 0.02967808670284336 50-51 0.02788002699109859 52-53 0.02539506806363111 54-55 0.02466776301168941 56-57 0.024950603865222295 58-59 0.021031237751980895 60-61 0.027576983219456216 62-63 0.021192861096856832 64-65 0.023152544153477526 66-67 0.016566392849783222 68-69 0.01806140878988561 70-71 0.03915325529619498 72-73 0.0356985562994719 74-75 0.03050640634533252 76-77 0.021940369066908022 78-79 0.015152188582118802 80-81 0.016829030785206617 82-83 0.022304021592878875 84-85 0.018707902169389348 86-87 0.009394356920913656 88-89 0.040143198283560076 90-91 0.05089115071780968 92-93 0.0327489302554861 94-95 0.05131541199810901 96-97 0.041496793796896025 98-99 0.032304466057077284 100-101 0.16406789796718238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.133027413165834 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62773461099891 40.183304962818355 2 13.339512528775971 14.175373697394456 3 4.5025732569261905 7.177060448701399 4 2.0671235261582304 4.3933012392706114 5 1.1376786728466666 3.0224156055868026 6 0.7068366302809449 2.2533822032008324 7 0.5117109781552409 1.903212740095822 8 0.33574723652565625 1.4271413697769892 9 0.2553801286299793 1.2212207437747056 >10 1.3790412111438575 13.289360696857976 >50 0.09205666922903263 3.3210350748262645 >100 0.03917973627225061 3.7431312070829357 >500 0.003056233271606913 1.1122286542841369 >1k 0.00213936329012277 1.7364845953321317 >5k 1.5281166358019785E-4 0.590154686546575 >10k+ 7.640583179009893E-5 0.45119207444993015 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11114 0.4490704637377823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8835 0.35698556299471895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5702 0.23039407812064377 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2554 0.10319650570328379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.040583621898347E-5 0.0 0.0 0.0 0.0 6 4.040583621898347E-5 0.0 0.0 0.0 0.0 7 1.2121750865695041E-4 0.0 0.0 0.0 0.0 8 1.2121750865695041E-4 0.0 0.0 0.0 0.0 9 1.2121750865695041E-4 0.0 0.0 0.0 0.0 10-11 1.2121750865695041E-4 0.0 0.0 4.040583621898347E-5 0.0 12-13 1.4142042676644216E-4 0.0 0.0 4.040583621898347E-5 6.060875432847521E-5 14-15 2.0202918109491735E-4 0.0 0.0 4.040583621898347E-5 1.0101459054745868E-4 16-17 2.0202918109491735E-4 0.0 0.0 4.040583621898347E-5 1.8182626298542562E-4 18-19 2.0202918109491735E-4 0.0 0.0 4.040583621898347E-5 2.4243501731390082E-4 20-21 2.0202918109491735E-4 0.0 0.0 1.0101459054745868E-4 3.232466897518678E-4 22-23 2.0202918109491735E-4 0.0 0.0 1.4142042676644216E-4 3.232466897518678E-4 24-25 2.4243501731390082E-4 0.0 0.0 3.434496078613595E-4 3.232466897518678E-4 26-27 2.4243501731390082E-4 0.0 0.0 0.0010909575779125538 3.6365252597085124E-4 28-29 3.232466897518678E-4 0.0 0.0 0.003454698996723087 4.040583621898347E-4 30-31 3.6365252597085124E-4 0.0 0.0 0.011152010796439439 4.2426128029932644E-4 32-33 3.6365252597085124E-4 0.0 0.0 0.02769820072811317 5.252758708467852E-4 34-35 4.040583621898347E-4 0.0 0.0 0.052588195839006986 5.252758708467852E-4 36-37 4.040583621898347E-4 0.0 0.0 0.09452945383431183 5.252758708467852E-4 38-39 4.040583621898347E-4 0.0 0.0 0.15491597606358262 6.060875432847521E-4 40-41 4.040583621898347E-4 0.0 0.0 0.22049464824699277 6.060875432847521E-4 42-43 4.2426128029932644E-4 0.0 0.0 0.28552784164144673 6.060875432847521E-4 44-45 4.8487003462780164E-4 0.0 0.0 0.3657132236180194 6.464933795037356E-4 46-47 5.252758708467852E-4 0.0 0.0 0.45797995062406816 6.86899215722719E-4 48-49 5.252758708467852E-4 0.0 0.0 0.5537013766268399 7.475079700511942E-4 50-51 5.252758708467852E-4 0.0 0.0 0.6614233359866499 8.889283968176364E-4 52-53 5.252758708467852E-4 0.0 0.0 0.7635692899482401 9.293342330366198E-4 54-55 5.252758708467852E-4 0.0 0.0 0.8704225238293419 9.293342330366198E-4 56-57 5.252758708467852E-4 0.0 0.0 0.9921046996028107 9.293342330366198E-4 58-59 5.252758708467852E-4 0.0 0.0 1.1238075227585873 9.293342330366198E-4 60-61 5.656817070657686E-4 0.0 0.0 1.262298526399153 9.293342330366198E-4 62-63 5.858846251752604E-4 0.0 0.0 1.3965267143186162 9.293342330366198E-4 64-65 6.060875432847521E-4 0.0 0.0 1.5335631078552987 9.293342330366198E-4 66-67 6.86899215722719E-4 0.0 0.0 1.6930853492478453 9.495371511461115E-4 68-69 7.273050519417025E-4 0.0 0.0 1.8707094052664968 9.697400692556033E-4 70-71 7.273050519417025E-4 0.0 0.0 2.0576874123698428 9.697400692556033E-4 72-73 7.273050519417025E-4 0.0 0.0 2.2453927245251304 0.0010101459054745869 74-75 7.475079700511942E-4 0.0 0.0 2.4346334584567395 0.0010707546598030622 76-77 7.67710888160686E-4 0.0 0.0 2.6386223226082777 0.0010909575779125538 78-79 7.67710888160686E-4 0.0 0.0 2.8619655823087085 0.0010909575779125538 80-81 9.293342330366198E-4 4.040583621898347E-5 0.0 3.1022590903030034 0.0010909575779125538 82-83 9.293342330366198E-4 4.040583621898347E-5 0.0 3.3443506580090427 0.0010909575779125538 84-85 9.495371511461115E-4 4.040583621898347E-5 0.0 3.5877958212284184 0.0010909575779125538 86-87 9.697400692556033E-4 4.040583621898347E-5 0.0 3.8427768506883133 0.0011111604960220454 88-89 9.697400692556033E-4 4.040583621898347E-5 0.0 4.116930449434117 0.0011313634141315373 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6395 0.0 43.445377 1 GTATCAA 10380 0.0 37.711834 1 ATCAACG 13040 0.0 29.84783 3 TATCAAC 13065 0.0 29.82709 2 TCAACGC 13130 0.0 29.67943 4 CAACGCA 13360 0.0 29.06177 5 AACGCAG 13785 0.0 28.2692 6 ACGCAGA 15230 0.0 25.31493 7 CGCAGAG 15330 0.0 25.025248 8 GAGTACT 9410 0.0 22.530016 12-13 GCAGAGT 16760 0.0 22.209295 9 GTACTTT 9720 0.0 21.772476 14-15 AGAGTAC 13705 0.0 21.557816 10-11 GTACATG 9150 0.0 21.2863 1 TACATGG 8925 0.0 21.192455 2 ACCGTAT 225 7.098788E-8 21.128298 8 AGTACTT 9740 0.0 20.351357 12-13 CAGAGTA 15815 0.0 20.064333 10-11 TGGTATC 3625 0.0 19.927006 2 CCGTATG 215 1.0714284E-6 19.899908 9 >>END_MODULE