FastQCFastQC Report
Thu 26 May 2016
SRR938185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474890
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107840.4357365377855177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92000.37173369321464794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59530.2405359430116086No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26530.10719668348896315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.039.1622621
GTATCAA108800.035.1929471
TATCAAC134000.028.2909532
ATCAACG134050.028.2043953
TCAACGC135500.027.8669594
CAACGCA136750.027.6122325
AACGCAG141600.026.733566
TAAGGTG12600.025.633045
GTAAGGT12900.024.6687344
ACGCAGA160100.023.6147567
CGCAGAG161150.023.3429998
GAGTACT95700.021.81222212-13
GTACATG93200.021.3063891
TACATGG91250.021.1369652
AGAGTAC139050.021.12621310-11
GCAGAGT175900.021.0075559
GTACTTT99500.020.57345214-15
AGTACTT99600.020.36205112-13
ACATGGG91700.020.0970783
AGGTAAG14750.019.968672
Produced by FastQC (version 0.11.5)