FastQCFastQC Report
Thu 26 May 2016
SRR938184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541196
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110260.4338901839921045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89580.3525111797751925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.2099798677473127No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26640.10483252767594471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.043.612641
GTATCAA98250.039.7849921
TATCAAC122450.031.7723122
ATCAACG124050.031.3631323
TCAACGC124650.031.2121664
CAACGCA127050.030.5851275
AACGCAG130900.029.788696
ACGCAGA146200.026.436817
CGCAGAG148200.026.0774678
TACATGG88750.025.669742
GTACATG92050.024.9822771
TAAGGTG15750.024.4606135
GTAAGGT15500.023.6277284
GCAGAGT160000.023.5600539
ACATGGG91000.023.5198233
ACCGTAT1703.1307918E-622.3699048
GAGTACT92950.022.19367212-13
AGAGTAC131900.021.76611710-11
GTACTTT95250.021.65647914-15
AGGTAAG16000.021.1053562
Produced by FastQC (version 0.11.5)