##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2541196 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936229633605592 33.0 31.0 34.0 30.0 34.0 2 32.05393051145996 33.0 31.0 34.0 30.0 34.0 3 32.07712431469277 34.0 31.0 34.0 30.0 34.0 4 35.59195827476511 37.0 35.0 37.0 33.0 37.0 5 35.59084383888531 37.0 35.0 37.0 33.0 37.0 6 35.62083247415784 37.0 35.0 37.0 33.0 37.0 7 35.55134747575551 37.0 35.0 37.0 33.0 37.0 8 35.545658028739226 37.0 35.0 37.0 33.0 37.0 9 37.17329123766919 39.0 37.0 39.0 34.0 39.0 10-11 37.082331508470816 39.0 37.0 39.0 33.0 39.0 12-13 36.93202865894642 39.0 37.0 39.0 33.0 39.0 14-15 38.10768256364326 40.0 38.0 41.0 33.0 41.0 16-17 37.92803290261751 40.0 38.0 41.0 33.0 41.0 18-19 38.095733072143986 40.0 38.0 41.0 33.0 41.0 20-21 38.09044658499384 40.0 38.0 41.0 33.0 41.0 22-23 37.97889556728407 40.0 38.0 41.0 33.0 41.0 24-25 37.85371533718769 40.0 38.0 41.0 32.5 41.0 26-27 37.667996093178175 40.0 38.0 41.0 32.0 41.0 28-29 37.49023117461227 40.0 37.0 41.0 32.0 41.0 30-31 37.26268595574682 40.0 37.0 41.0 31.0 41.0 32-33 36.97361104771139 40.0 36.0 41.0 30.5 41.0 34-35 36.73012766429666 39.0 36.0 41.0 30.0 41.0 36-37 36.46719831921662 39.0 35.5 41.0 30.0 41.0 38-39 36.16175336337693 39.0 35.0 40.5 29.5 41.0 40-41 36.05043491332428 39.0 35.0 41.0 29.0 41.0 42-43 35.83568583454405 39.0 35.0 40.5 28.5 41.0 44-45 35.493184311639084 39.0 35.0 40.0 26.5 41.0 46-47 35.10703641120165 38.0 34.5 40.0 25.5 41.0 48-49 34.685449489138186 38.0 34.0 40.0 24.0 41.0 50-51 33.93435945121904 37.5 33.5 39.5 23.0 40.5 52-53 33.99730127074024 38.0 33.5 39.5 23.0 40.5 54-55 34.06013290592304 38.0 33.5 40.0 22.5 41.0 56-57 33.721249954745716 38.0 33.0 40.0 20.5 41.0 58-59 33.291752780974 37.0 33.0 40.0 19.0 41.0 60-61 32.76335217747864 36.5 32.0 39.5 15.0 41.0 62-63 32.20318503570759 36.0 31.0 39.0 10.0 41.0 64-65 31.6419624853809 35.0 31.0 39.0 7.5 40.0 66-67 31.05785641878863 35.0 30.0 38.0 3.5 40.0 68-69 30.365785834701455 35.0 29.0 37.5 2.0 40.0 70-71 29.758063525993272 34.0 29.0 37.0 2.0 39.0 72-73 29.064238059559358 34.0 28.0 36.0 2.0 39.0 74-75 28.38862094856123 34.0 26.0 36.0 2.0 38.5 76-77 27.65050846137016 33.5 26.0 35.0 2.0 37.5 78-79 26.893950525658 33.0 24.5 35.0 2.0 37.0 80-81 26.186433671389377 32.0 22.5 35.0 2.0 36.0 82-83 25.461989551376597 32.0 20.0 35.0 2.0 36.0 84-85 24.747594833298965 31.0 17.5 34.0 2.0 35.0 86-87 24.01526131789913 31.0 9.0 34.0 2.0 35.0 88-89 23.13811331357361 30.0 3.5 34.0 2.0 35.0 90-91 22.234361300741853 29.5 2.0 34.0 2.0 35.0 92-93 21.30193578141946 29.0 2.0 34.0 2.0 35.0 94-95 20.212516665381184 28.0 2.0 34.0 2.0 35.0 96-97 18.94136815892989 25.5 2.0 33.0 2.0 35.0 98-99 17.377175550410122 21.0 2.0 33.0 2.0 34.0 100-101 13.497561384481951 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29346166143326613 1101 2 0.28695543602243134 1101 3 0.22285959022005386 1101 4 0.07477978430544852 1101 5 0.21587827917129943 1101 6 0.278849013997565 1101 7 0.22250837680363844 1101 8 0.12829844680853597 1101 9 0.22198355780277268 1101 10-11 0.29044915687563133 1101 12-13 0.3352181402310137 1101 14-15 0.4068855898550936 1101 16-17 0.5151309184192954 1101 18-19 0.4994066609697114 1101 20-21 0.4197671728913406 1101 22-23 0.29629534109029265 1101 24-25 0.3676332567943561 1101 26-27 0.42430138949673335 1101 28-29 0.46071131048926617 1101 30-31 0.31463561918910443 1101 32-33 0.21519960861831322 1101 34-35 0.4711103427148018 1101 36-37 0.24510309518102957 1101 38-39 0.19606542023822016 1101 40-41 0.40961109335489 1101 42-43 0.40281941810874144 1101 44-45 0.5059159457341877 1101 46-47 0.4483158071893101 1101 48-49 0.5338962110681322 1101 50-51 0.48632184760377584 1101 52-53 0.49694266146296684 1101 54-55 0.5813071442393749 1101 56-57 0.43088010589728754 1101 58-59 0.5235832842900976 1101 60-61 0.5288508062375854 1101 62-63 0.49913841966322536 1101 64-65 0.4142682512391396 1101 66-67 0.34269373898845146 1101 68-69 0.31776288171741385 1101 70-71 0.2886911962063685 1101 72-73 0.2803101446442753 1101 74-75 0.23946651070732727 1101 76-77 0.10315196551718486 1101 78-79 -0.114837650857325 1101 80-81 -0.019349217920957784 1101 82-83 -0.1703653100244118 1101 84-85 -0.06083701144697429 1101 86-87 0.3952734569463594 1101 88-89 0.14970450507636102 1101 90-91 -0.19623813829003822 1101 92-93 -0.14694057489066026 1101 94-95 -0.2939384466651198 1101 96-97 -0.2438670403121428 1101 98-99 -0.36941636367512487 1101 100-101 -0.5792700460765694 1102 1 0.1651408802510339 1102 2 0.27693575356298794 1102 3 0.13075125790087583 1102 4 0.0815079401299954 1102 5 0.12469302533544635 1102 6 0.1916222904756566 1102 7 0.1381386523736694 1102 8 0.17385811360314563 1102 9 0.28510043869074053 1102 10-11 0.24538671987042449 1102 12-13 0.24503514718657726 1102 14-15 0.29442769690021464 1102 16-17 0.3494288716931564 1102 18-19 0.19015937657182036 1102 20-21 0.2528918237870812 1102 22-23 0.3402250420230004 1102 24-25 0.29973715802476164 1102 26-27 0.2986617875770108 1102 28-29 0.29369807495194067 1102 30-31 0.3478592284669304 1102 32-33 0.21193363095473927 1102 34-35 0.28145701300122994 1102 36-37 0.1262143701176086 1102 38-39 0.08891254689558536 1102 40-41 0.21277840518184377 1102 42-43 0.4065926696229454 1102 44-45 0.25674755499953505 1102 46-47 0.3015505214933043 1102 48-49 0.13733209914257571 1102 50-51 0.11517125845619347 1102 52-53 0.07771407657173057 1102 54-55 0.05433581681623423 1102 56-57 0.029362711533508445 1102 58-59 0.09194930860491723 1102 60-61 0.1660296693200678 1102 62-63 -0.06641634016864373 1102 64-65 -0.05454971682581444 1102 66-67 -0.020852618157306324 1102 68-69 -0.08104173847274865 1102 70-71 -0.46461349497483084 1102 72-73 -0.43205700070479836 1102 74-75 -0.4650057099546494 1102 76-77 -0.54918358102017 1102 78-79 -0.5168582234351256 1102 80-81 -0.680030794265182 1102 82-83 -0.7035260220254891 1102 84-85 -0.7678806093038233 1102 86-87 -0.8001316093171411 1102 88-89 -0.6840363441012371 1102 90-91 -0.8434677911672814 1102 92-93 -1.1727744855137274 1102 94-95 -1.1422361635622345 1102 96-97 -1.3610546134485126 1102 98-99 -1.2856294873430656 1102 100-101 -1.5042035602420114 1103 1 0.3722087696994727 1103 2 0.39314526930321847 1103 3 0.3844163215062153 1103 4 0.3464247639205382 1103 5 0.30987942596553353 1103 6 0.3563651841008024 1103 7 0.3060543544190679 1103 8 0.26149296169020886 1103 9 0.30605857611645604 1103 10-11 0.4317597538330489 1103 12-13 0.382900907705924 1103 14-15 0.44018221968118354 1103 16-17 0.37882993138431686 1103 18-19 0.3649442759727677 1103 20-21 0.4927737422099554 1103 22-23 0.36406138059910376 1103 24-25 0.37046378634524757 1103 26-27 0.36159959635526207 1103 28-29 0.34360827567271457 1103 30-31 0.41317701648096516 1103 32-33 0.36735201802842 1103 34-35 0.3090173366366358 1103 36-37 0.26266452405922536 1103 38-39 0.3095868171645648 1103 40-41 0.44643777056048606 1103 42-43 0.5467198206289439 1103 44-45 0.5344585385552278 1103 46-47 0.6576978937456275 1103 48-49 0.5011608898058384 1103 50-51 0.5813600398113579 1103 52-53 0.6979213010878311 1103 54-55 0.764421379717497 1103 56-57 0.6169759302260829 1103 58-59 0.7291639497658053 1103 60-61 0.4970459226912709 1103 62-63 0.53524330564629 1103 64-65 0.5748954966637747 1103 66-67 0.42274612524946065 1103 68-69 0.2622078020890797 1103 70-71 0.1437326171042166 1103 72-73 0.0760875850848386 1103 74-75 0.027120444607508176 1103 76-77 -0.0826188957054832 1103 78-79 -0.14758106288237016 1103 80-81 0.04963757490830645 1103 82-83 -0.12068469526350967 1103 84-85 -0.08402180599109599 1103 86-87 -0.44328165466428615 1103 88-89 -0.4810340349769149 1103 90-91 -0.5966786374881963 1103 92-93 -0.7489994550834069 1103 94-95 -0.7227265988948197 1103 96-97 -0.9341209455805419 1103 98-99 -1.1350329635596843 1103 100-101 -1.4477575531774036 1104 1 0.18749550689240735 1104 2 0.08987839449554258 1104 3 0.1306506740466915 1104 4 0.16147228414332915 1104 5 0.09791720749334587 1104 6 0.14451431919621882 1104 7 0.12487489883613989 1104 8 0.18386125887285942 1104 9 0.25271459686685915 1104 10-11 0.20217767747190152 1104 12-13 0.24285307133735756 1104 14-15 0.2754674604507841 1104 16-17 0.16169117711107361 1104 18-19 0.24694427035239386 1104 20-21 0.11933769779779624 1104 22-23 0.1649839466630354 1104 24-25 0.24643725187903698 1104 26-27 0.33955308288113173 1104 28-29 0.20196074168001843 1104 30-31 0.163570354516672 1104 32-33 0.28173306782910856 1104 34-35 0.22462731532139202 1104 36-37 0.16705879226606868 1104 38-39 -0.006348370141267878 1104 40-41 0.13957702083132517 1104 42-43 0.21057273134796617 1104 44-45 0.030457274674674295 1104 46-47 -0.07893651668465651 1104 48-49 -0.15109957005278574 1104 50-51 -0.20008004283617709 1104 52-53 -0.1254451561264034 1104 54-55 -0.021550652622110533 1104 56-57 -0.03687913379695118 1104 58-59 -0.13154810536439498 1104 60-61 -0.22662018802719786 1104 62-63 -0.12476325262218779 1104 64-65 -0.27371941600271654 1104 66-67 -0.37413469271099586 1104 68-69 -0.2748101458578702 1104 70-71 -0.3371813989728487 1104 72-73 -0.48053140094432933 1104 74-75 -0.6206939518280521 1104 76-77 -0.7093943176898385 1104 78-79 -0.9206393306564351 1104 80-81 -0.828965433113364 1104 82-83 -0.7142050943940426 1104 84-85 -0.7977093900331802 1104 86-87 -0.8397397857228697 1104 88-89 -0.951529027930377 1104 90-91 -1.0572697492956102 1104 92-93 -1.2877112418409737 1104 94-95 -1.424906213253177 1104 96-97 -1.6051270935620572 1104 98-99 -1.8661979642552033 1104 100-101 -1.6949520228744817 1105 1 0.14657696813438648 1105 2 0.22129500497877075 1105 3 0.34423825532215346 1105 4 0.2413053440844095 1105 5 0.20854624762254304 1105 6 0.2287764816700033 1105 7 0.2379032006500026 1105 8 0.2566976921229056 1105 9 0.30700317638452645 1105 10-11 0.3167372006920388 1105 12-13 0.3856152273141973 1105 14-15 0.31317626505200025 1105 16-17 0.35618475065238897 1105 18-19 0.34639759001638737 1105 20-21 0.2999881054518809 1105 22-23 0.361025518514154 1105 24-25 0.3040098280235597 1105 26-27 0.3669879255820305 1105 28-29 0.3497076117595128 1105 30-31 0.19674807932841532 1105 32-33 0.13326125458959837 1105 34-35 0.06882860066247076 1105 36-37 0.2848591362583832 1105 38-39 0.24438672962455854 1105 40-41 0.2229624256160676 1105 42-43 0.2672918236451167 1105 44-45 0.22997059644070816 1105 46-47 0.41262815490663485 1105 48-49 0.4995949361856802 1105 50-51 0.434384618508048 1105 52-53 0.48275041059328316 1105 54-55 0.3962770607500943 1105 56-57 0.46535778667411165 1105 58-59 0.3736519123335924 1105 60-61 0.36084554062415464 1105 62-63 0.3229014435258861 1105 64-65 0.13727516324817302 1105 66-67 0.13423287016579621 1105 68-69 -0.03489327048049162 1105 70-71 -0.2563697685654027 1105 72-73 -0.22875777795391983 1105 74-75 -0.24410723916854593 1105 76-77 -0.36560431320448217 1105 78-79 -0.30298331737833806 1105 80-81 -0.35164862908717254 1105 82-83 -0.470914334372349 1105 84-85 -0.3701936727884778 1105 86-87 -0.35268209768215186 1105 88-89 -0.16941429266086772 1105 90-91 -0.3582581720808129 1105 92-93 -0.5892378413748034 1105 94-95 -0.9197207519861976 1105 96-97 -1.009044944631512 1105 98-99 -0.9693046191429495 1105 100-101 -0.8840867738122995 1106 1 0.27461832296888744 1106 2 0.25468063357146775 1106 3 0.31772413114716613 1106 4 0.24976976305283216 1106 5 0.2275544814529198 1106 6 0.2458847652889773 1106 7 0.3314343174579193 1106 8 0.21407141279280495 1106 9 0.5077031036439834 1106 10-11 0.44839036764994233 1106 12-13 0.554092448048074 1106 14-15 0.6267614258385379 1106 16-17 0.17705296481680222 1106 18-19 0.5934663171780485 1106 20-21 0.673322976042499 1106 22-23 0.7343873861463237 1106 24-25 0.6565532344550249 1106 26-27 0.5081032437568709 1106 28-29 0.5410098064853415 1106 30-31 0.4981250531863708 1106 32-33 0.6818757093937009 1106 34-35 0.6231029896828915 1106 36-37 0.6849979705492899 1106 38-39 0.6958882819099017 1106 40-41 0.7013163224385366 1106 42-43 0.41540917648807607 1106 44-45 0.6608915822479275 1106 46-47 0.659233121007972 1106 48-49 0.7121817588495034 1106 50-51 0.7439120259402756 1106 52-53 0.8483889859620746 1106 54-55 0.8363443412979947 1106 56-57 0.5833259483413045 1106 58-59 0.5662306305390032 1106 60-61 0.9383670553951902 1106 62-63 0.748127835548587 1106 64-65 0.6086790593043894 1106 66-67 0.5295918916148139 1106 68-69 0.44744416010681576 1106 70-71 0.4273491086570438 1106 72-73 0.36954566736753947 1106 74-75 0.39525131112418066 1106 76-77 0.1275171435451874 1106 78-79 0.15237890094807582 1106 80-81 0.30545918408830985 1106 82-83 0.446529068958462 1106 84-85 0.16247978632196336 1106 86-87 0.16465597263703202 1106 88-89 -0.17920584342439483 1106 90-91 -0.16285572370753343 1106 92-93 -0.21909886808922607 1106 94-95 -0.4456020940189305 1106 96-97 -0.40591009806202294 1106 98-99 -0.4477449421380477 1106 100-101 -0.8756690427969236 1107 1 -0.14204489754732563 1107 2 -0.10111339559543353 1107 3 -0.021303227348973053 1107 4 -0.014538489525705245 1107 5 0.024534537759734576 1107 6 -0.07401645819289371 1107 7 0.07942685276783124 1107 8 0.0552764101704426 1107 9 -0.046891796447191325 1107 10-11 0.01752818675526413 1107 12-13 -0.005610959833695972 1107 14-15 -0.06048204108071076 1107 16-17 -0.04026210326272661 1107 18-19 0.07008156071586313 1107 20-21 -0.05642974132918255 1107 22-23 0.0065705848172186165 1107 24-25 0.10157269371791244 1107 26-27 0.01721978862673268 1107 28-29 0.04843822994043734 1107 30-31 0.04397313112056622 1107 32-33 0.06593145432342595 1107 34-35 0.10580659645678026 1107 36-37 0.07863368553361028 1107 38-39 -0.12308018351601646 1107 40-41 -0.11062806785211166 1107 42-43 -0.017189536030116415 1107 44-45 -0.021966740271054164 1107 46-47 -0.03213062903387254 1107 48-49 0.09409728024550645 1107 50-51 -0.0042245912790548346 1107 52-53 -0.01385623685288806 1107 54-55 -0.14608439395165362 1107 56-57 -0.25150875787273463 1107 58-59 -0.24333279799341057 1107 60-61 -0.11662365969088029 1107 62-63 -0.029284322076364333 1107 64-65 -0.10209373829367152 1107 66-67 -0.1636569027248349 1107 68-69 -0.12058580367906657 1107 70-71 -0.13533899372185232 1107 72-73 -0.16394960221660426 1107 74-75 -0.25499182299540024 1107 76-77 -0.5407222805472784 1107 78-79 -0.5789782456130972 1107 80-81 -0.6351885871713208 1107 82-83 -0.7620418026025284 1107 84-85 -0.758196722550398 1107 86-87 -0.7258371813389388 1107 88-89 -0.8841106673129779 1107 90-91 -1.0016873080374609 1107 92-93 -1.044855265293183 1107 94-95 -0.8836224217117348 1107 96-97 -0.9505835309989514 1107 98-99 -1.305488836051751 1107 100-101 -1.324915615846452 1108 1 -0.0464368499077743 1108 2 0.05102657326191462 1108 3 -0.03605513694605378 1108 4 -0.0016323490913805472 1108 5 -0.05275261156965172 1108 6 -0.09412588209684003 1108 7 0.012261333928783813 1108 8 -0.04898939133895652 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2316 54-55 -0.517584284359188 2316 56-57 -0.47121267717353277 2316 58-59 -0.34278185119273274 2316 60-61 -0.4921348058324426 2316 62-63 -0.3219435589604913 2316 64-65 -0.2090537898804179 2316 66-67 -0.0862690833410582 2316 68-69 -0.1485795862632564 2316 70-71 -0.38431829150808383 2316 72-73 -0.35494445234890293 2316 74-75 -0.2175734547098962 2316 76-77 -0.46243937442888594 2316 78-79 -0.24521547124051324 2316 80-81 0.06744446983242014 2316 82-83 -0.0188657687149707 2316 84-85 -0.21037428584005013 2316 86-87 -0.14148008796615485 2316 88-89 -0.3532343336117272 2316 90-91 -0.2642675698460515 2316 92-93 -0.40854669203061533 2316 94-95 -0.46180123944380114 2316 96-97 -0.37734799426981525 2316 98-99 -0.40712252223370626 2316 100-101 -0.37393904417297463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8702.0 3 2531.0 4 2543.0 5 2773.0 6 3197.0 7 3993.0 8 5226.0 9 6537.0 10 7967.0 11 10072.0 12 12556.0 13 15393.0 14 17330.0 15 21448.0 16 25536.0 17 31416.0 18 31028.0 19 27485.0 20 28126.0 21 30199.0 22 32853.0 23 36825.0 24 41006.0 25 45703.0 26 51375.0 27 59240.0 28 67742.0 29 78085.0 30 91337.0 31 108852.0 32 132106.0 33 164231.0 34 210096.0 35 273698.0 36 342107.0 37 344171.0 38 158734.0 39 8977.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.398668278772384 17.744539044433267 14.504813459716537 23.35197921707781 2 15.860845969006624 19.06223755656376 40.038026122189926 25.038890352239697 3 19.861176742296124 22.891807007926058 29.03417651725615 28.212839732521665 4 13.306621454269926 15.93630599850982 35.57328029308729 35.18379225413297 5 14.557005696252192 36.431607713656774 33.428448298635494 15.582938291455543 6 33.63988868113644 34.779022277178925 16.492872233293298 15.08821680839134 7 29.757448861504308 32.19001573492175 19.35083987903976 18.70169552453418 8 27.236924128659197 34.21056465900683 18.7972628082875 19.755248404046483 9 26.67487207288418 16.74036823467672 19.468127355048768 37.11663233739033 10-11 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28.189259199520087 22.84792764496973 36-37 23.42822696644437 25.258827671933414 28.200833573590323 23.112111788031893 38-39 22.9543841620724 25.632964906426654 28.152520668030917 23.260130263470025 40-41 23.30906870118395 25.511766253116587 28.210460081681 22.968704964018468 42-43 23.03549387972399 25.488034201328148 27.988988602510563 23.4874833164373 44-45 23.052444482936448 25.762751898465087 28.13836257632243 23.046441042276033 46-47 23.507511974334538 26.041064816628452 27.434400765538484 23.017022443498522 48-49 23.3356114391356 26.13754378726691 27.253297282571467 23.273547491026022 50-51 23.310241185024665 26.337549581252624 27.327078091567902 23.02513114215481 52-53 23.582887963652716 26.101257925382576 27.207992151128174 23.10786195983653 54-55 23.154842063799684 26.192363636435218 27.3157844073079 23.3370098924572 56-57 23.293016426900635 26.4125432125007 27.156157406177332 23.138282954421328 58-59 23.33305707862019 26.0110282718811 27.143659472035903 23.512255177462812 60-61 23.045941423022402 26.239905866161617 27.428605396476595 23.28554731433939 62-63 23.027769548086603 26.17282613938024 27.60470053712269 23.19470377541047 64-65 22.867460919992496 26.288837655063734 27.44655894965978 23.39714247528399 66-67 22.882417652281795 26.269191274321674 27.313084797379155 23.535306276017376 68-69 23.201836506070826 25.940471057506976 27.503371587313772 23.354320849108422 70-71 23.245045641304174 26.05169695410317 27.096518527894947 23.606738876697708 72-73 23.090171692610067 26.1768038590678 27.085525031024986 23.64749941729714 74-75 23.054587357231622 26.639645255896017 26.88609375003076 23.41967363684161 76-77 23.28875121524976 26.216174728315416 26.954160975820958 23.540913080613862 78-79 23.353012233613565 26.40214624523198 26.906589446761682 23.338252074392777 80-81 23.508436525363503 26.39497931632783 26.619519033524693 23.477065124783977 82-83 23.416528249010085 26.375648463760815 26.777026867457547 23.430796419771553 84-85 23.36227835157452 26.538417486034245 26.635399434560604 23.46390472783063 86-87 23.64074628989396 26.39148041924833 26.515388425819232 23.45238486503848 88-89 23.49148377773359 26.468332174998476 26.27772866612983 23.76245538113811 90-91 23.50366786548803 26.456396590775018 26.340617983581026 23.699317560155926 92-93 23.594544945792677 26.147547649611404 26.520265447220993 23.73764195737493 94-95 23.504855384044664 25.997260262643817 26.6236350293359 23.874249323975615 96-97 23.726108473314834 25.61640261904785 26.631003092177608 24.026485815459704 98-99 23.503624257632065 25.912177186716157 26.25205161980761 24.33214693584417 100-101 24.574957036168424 24.64160687783811 26.23574578966271 24.547690296330753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 835.0 1 572.0 2 434.5 3 587.5 4 514.0 5 489.5 6 899.5 7 2040.0 8 3425.0 9 3994.5 10 3402.5 11 2618.5 12 2488.0 13 2646.5 14 2783.0 15 2969.5 16 3210.0 17 3362.5 18 3579.5 19 3934.5 20 4384.5 21 5004.5 22 5858.0 23 6960.5 24 8460.0 25 10420.0 26 12883.5 27 15905.5 28 18486.5 29 20691.5 30 23357.0 31 27514.0 32 33325.0 33 35943.5 34 36196.0 35 38596.5 36 42662.5 37 46684.0 38 50804.0 39 54797.5 40 59183.5 41 64522.0 42 68016.5 43 71121.0 44 75418.5 45 81632.0 46 87162.0 47 92541.0 48 97977.0 49 99108.5 50 97893.5 51 96563.5 52 97700.0 53 101201.5 54 103240.0 55 99274.5 56 92169.5 57 84829.5 58 78864.5 59 71545.5 60 59862.5 61 50366.5 62 42213.0 63 32391.5 64 23620.5 65 17665.0 66 13247.5 67 10100.5 68 7740.5 69 5690.5 70 4243.0 71 3093.0 72 2182.5 73 1650.5 74 1217.5 75 849.0 76 594.0 77 394.5 78 256.0 79 173.5 80 113.5 81 62.5 82 46.0 83 39.0 84 31.0 85 18.0 86 10.0 87 13.0 88 10.5 89 7.0 90 5.5 91 5.0 92 3.5 93 4.0 94 3.0 95 2.0 96 2.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14670257626723796 2 0.053636161870237474 3 0.15225114473657286 4 0.12785318409127042 5 0.19545914600841494 6 0.1990401370063545 7 0.16385985181780546 8 0.07102954671737245 9 0.11931389786541456 10-11 0.1016647279469982 12-13 0.14928010275476586 14-15 0.13558576355385418 16-17 0.13235893650076577 18-19 0.13054876522708206 20-21 0.13275245199504485 22-23 0.19288161952088703 24-25 0.21387567114067552 26-27 0.14729284950865656 28-29 0.12742031704756343 30-31 0.10085802118372608 32-33 0.12354418942891457 34-35 0.09371571496256093 36-37 0.08846228311393532 38-39 0.05286880665639329 40-41 0.06138841710753519 42-43 0.06475297458362125 44-45 0.03880062773591648 46-47 0.050547065240146766 48-49 0.04226356408557231 50-51 0.05113733848156537 52-53 0.0276838150225327 54-55 0.04842208157103978 56-57 0.027487057275393162 58-59 0.049956791998728156 60-61 0.03868257308763275 62-63 0.050114198196439794 64-65 0.03897770970834206 66-67 0.03724624153351414 68-69 0.06260831513980031 70-71 0.052593345810397936 72-73 0.051471826651702586 74-75 0.04373924718911882 76-77 0.02227297697619546 78-79 0.02254843782219081 80-81 0.02618845614427223 82-83 0.02266649247047453 84-85 0.02034475105422801 86-87 0.023669956980886166 88-89 0.0641823771169166 90-91 0.10744940571290054 92-93 0.07907693857537947 94-95 0.07482697123716549 96-97 0.06782239543899801 98-99 0.056449797654332834 100-101 0.13007654663394716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2541196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.4705991964014 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26645409587496 40.78012999091277 2 12.997801365615159 13.900004545104938 3 4.376557716470835 7.020514905519894 4 1.9987835640658795 4.275046193380854 5 1.0689165374384224 2.857780387388754 6 0.709746899423051 2.277035519396315 7 0.477043751214417 1.7855470654233738 8 0.3288133275591092 1.4065476518678541 9 0.25984068216024275 1.250445327363888 >10 1.3779658688946346 13.415967725634244 >50 0.09406896666821467 3.421253314220091 >100 0.038699842347897306 3.7425122483238744 >500 0.002801118418754497 1.0172649969236371 >1k 0.002285122920559497 1.8519591284202952 >5k 1.474272851973869E-4 0.5633998165759949 >10k+ 7.371364259869344E-5 0.43459118354322934 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11026 0.4338901839921045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8958 0.3525111797751925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5336 0.2099798677473127 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2664 0.10483252767594471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.935154942790717E-5 2 0.0 0.0 0.0 0.0 3.935154942790717E-5 3 0.0 0.0 0.0 0.0 3.935154942790717E-5 4 0.0 0.0 0.0 0.0 3.935154942790717E-5 5 0.0 0.0 0.0 0.0 3.935154942790717E-5 6 3.935154942790717E-5 0.0 0.0 0.0 3.935154942790717E-5 7 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 3.935154942790717E-5 8 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 3.935154942790717E-5 9 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 3.935154942790717E-5 10-11 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 3.935154942790717E-5 12-13 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 9.837887356976794E-5 14-15 7.870309885581434E-5 0.0 0.0 3.935154942790717E-5 1.5740619771162872E-4 16-17 7.870309885581434E-5 0.0 0.0 5.902732414186076E-5 3.9351549427907176E-4 18-19 9.837887356976794E-5 0.0 0.0 1.1805464828372152E-4 3.9351549427907176E-4 20-21 1.1805464828372152E-4 0.0 0.0 1.574061977116287E-4 3.9351549427907176E-4 22-23 1.574061977116287E-4 0.0 0.0 1.9675774713953588E-4 4.1319126899302537E-4 24-25 2.3610929656744305E-4 0.0 0.0 4.3286704370697893E-4 4.3286704370697893E-4 26-27 2.3610929656744305E-4 0.0 0.0 0.0010428160598395402 4.3286704370697893E-4 28-29 2.3610929656744305E-4 0.0 0.0 0.0031677997289465275 4.722185931348861E-4 30-31 2.754608459953502E-4 0.0 0.0 0.01058556679610703 5.115701425627932E-4 32-33 2.754608459953502E-4 0.0 0.0 0.027723166571960604 5.509216919907004E-4 34-35 2.754608459953502E-4 0.0 0.0 0.052435939612686305 6.296247908465148E-4 36-37 2.754608459953502E-4 0.0 0.0 0.09432566397869349 6.296247908465148E-4 38-39 3.5416394485116454E-4 0.0 0.0 0.15496640164709846 6.68976340274422E-4 40-41 3.5416394485116454E-4 0.0 0.0 0.220447379895136 6.68976340274422E-4 42-43 3.7383971956511815E-4 0.0 0.0 0.28622349476388287 7.083278897023291E-4 44-45 4.3286704370697893E-4 0.0 0.0 0.3649069178449832 7.280036644162827E-4 46-47 4.3286704370697893E-4 0.0 0.0 0.459527718444386 7.476794391302363E-4 48-49 4.3286704370697893E-4 0.0 0.0 0.5596577359636958 7.476794391302363E-4 50-51 4.3286704370697893E-4 0.0 0.0 0.6632900413820895 7.870309885581435E-4 52-53 4.3286704370697893E-4 0.0 0.0 0.7620427546714224 8.263825379860506E-4 54-55 4.3286704370697893E-4 3.935154942790717E-5 0.0 0.8659701967105253 8.263825379860506E-4 56-57 4.3286704370697893E-4 3.935154942790717E-5 0.0 0.988196109233605 8.657340874139579E-4 58-59 4.3286704370697893E-4 5.902732414186076E-5 0.0 1.117957843472129 8.657340874139579E-4 60-61 4.3286704370697893E-4 7.870309885581434E-5 0.0 1.2550389659042436 9.05085636841865E-4 62-63 4.3286704370697893E-4 9.837887356976794E-5 0.0 1.3893654798764046 9.444371862697722E-4 64-65 4.3286704370697893E-4 1.1805464828372152E-4 0.0 1.530814624295017 9.444371862697722E-4 66-67 4.3286704370697893E-4 1.1805464828372152E-4 0.0 1.6913886217355922 9.444371862697722E-4 68-69 4.3286704370697893E-4 1.1805464828372152E-4 0.0 1.869965953039435 9.444371862697722E-4 70-71 4.525428184209325E-4 1.1805464828372152E-4 0.0 2.0528326032309194 9.444371862697722E-4 72-73 4.918943678488397E-4 1.1805464828372152E-4 0.0 2.241680688935446 9.837887356976794E-4 74-75 5.115701425627933E-4 1.574061977116287E-4 0.0 2.431197750980247 0.0010231402851255866 76-77 5.115701425627933E-4 1.574061977116287E-4 0.0 2.636278350823785 0.0010428160598395402 78-79 5.115701425627933E-4 1.574061977116287E-4 0.0 2.8621759203146864 0.0010624918345534936 80-81 5.70597466704654E-4 1.574061977116287E-4 0.0 3.0979704044867065 0.0010624918345534936 82-83 6.296247908465148E-4 1.574061977116287E-4 0.0 3.3433273151697076 0.0010624918345534936 84-85 6.296247908465148E-4 1.574061977116287E-4 0.0 3.588566171204425 0.0010624918345534936 86-87 6.296247908465148E-4 1.574061977116287E-4 0.0 3.841793391773008 0.0010624918345534936 88-89 6.296247908465148E-4 1.574061977116287E-4 0.0 4.108911709289641 0.0010624918345534936 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5775 0.0 43.61264 1 GTATCAA 9825 0.0 39.784992 1 TATCAAC 12245 0.0 31.772312 2 ATCAACG 12405 0.0 31.363132 3 TCAACGC 12465 0.0 31.212166 4 CAACGCA 12705 0.0 30.585127 5 AACGCAG 13090 0.0 29.78869 6 ACGCAGA 14620 0.0 26.43681 7 CGCAGAG 14820 0.0 26.077467 8 TACATGG 8875 0.0 25.66974 2 GTACATG 9205 0.0 24.982277 1 TAAGGTG 1575 0.0 24.460613 5 GTAAGGT 1550 0.0 23.627728 4 GCAGAGT 16000 0.0 23.560053 9 ACATGGG 9100 0.0 23.519823 3 ACCGTAT 170 3.1307918E-6 22.369904 8 GAGTACT 9295 0.0 22.193672 12-13 AGAGTAC 13190 0.0 21.766117 10-11 GTACTTT 9525 0.0 21.656479 14-15 AGGTAAG 1600 0.0 21.105356 2 >>END_MODULE