FastQCFastQC Report
Thu 26 May 2016
SRR938184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541196
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107000.4210615788786068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.3568004986628343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57180.22501215962877322No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25910.1019598645677075No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25600.10073996653544237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67200.045.099491
GTATCAA107700.037.3142661
TATCAAC130650.030.5021232
ATCAACG130800.030.3921223
TCAACGC131100.030.2845614
CAACGCA134000.029.6291475
AACGCAG138350.028.8005316
ACGCAGA156050.025.321297
CGCAGAG158000.024.8885448
GAGTACT98400.023.79598412-13
TAAGGTG15000.023.4292375
GTACTTT100650.023.02718414-15
GTAAGGT15000.022.4794044
GCAGAGT172600.022.3980319
GTACATG91500.022.0641961
GTGTAAG11100.021.8256631
TACATGG88850.021.8112342
AGAGTAC141100.020.91979610-11
CAGAGTA161900.020.63766510-11
AGTACTT100650.020.26755112-13
Produced by FastQC (version 0.11.5)