##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2541196 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51142178722145 34.0 31.0 34.0 31.0 34.0 2 32.724651699436016 34.0 31.0 34.0 31.0 34.0 3 32.778833667296816 34.0 31.0 34.0 31.0 34.0 4 36.234726089605054 37.0 35.0 37.0 35.0 37.0 5 36.20662868979803 37.0 37.0 37.0 35.0 37.0 6 36.23622066145232 37.0 37.0 37.0 35.0 37.0 7 36.19816141690763 37.0 37.0 37.0 35.0 37.0 8 36.18344865960752 37.0 37.0 37.0 35.0 37.0 9 37.87066523007277 39.0 38.0 39.0 35.0 39.0 10-11 37.84962690795987 39.0 38.0 39.0 35.0 39.0 12-13 37.765944264039454 39.0 38.0 39.0 35.0 39.0 14-15 39.0476989968503 40.0 38.0 41.0 36.0 41.0 16-17 38.93178172797376 40.0 38.0 41.0 35.5 41.0 18-19 39.178409890461026 40.0 39.0 41.0 36.0 41.0 20-21 39.154308640498414 40.0 39.0 41.0 36.0 41.0 22-23 39.05988971334757 40.0 39.0 41.0 35.5 41.0 24-25 38.95741021156967 40.0 38.0 41.0 35.0 41.0 26-27 38.78278043094669 40.0 38.0 41.0 35.0 41.0 28-29 38.665813262731405 40.0 38.0 41.0 34.5 41.0 30-31 38.427467224094485 40.0 38.0 41.0 34.0 41.0 32-33 38.21642702884783 40.0 38.0 41.0 34.0 41.0 34-35 38.06177858772011 40.0 38.0 41.0 33.0 41.0 36-37 38.070023327598506 40.0 38.0 41.0 33.0 41.0 38-39 37.92459652856371 40.0 37.5 41.0 33.0 41.0 40-41 37.74736836513201 40.0 37.0 41.0 33.0 41.0 42-43 37.514820777303285 40.0 37.0 41.0 33.0 41.0 44-45 37.233527047893986 40.0 36.5 41.0 32.0 41.0 46-47 36.83876883168398 39.5 36.0 41.0 31.0 41.0 48-49 36.42562793267422 39.0 35.5 41.0 30.0 41.0 50-51 36.029353107749266 39.0 35.0 40.5 29.5 41.0 52-53 35.491718466422896 38.0 35.0 40.0 28.0 41.0 54-55 35.16408081076784 38.0 34.0 40.0 27.0 41.0 56-57 34.794701786088126 38.0 34.0 40.0 26.0 41.0 58-59 34.32559314590453 37.5 33.5 40.0 25.5 41.0 60-61 33.82253297266327 37.0 33.0 39.5 24.5 41.0 62-63 33.33384064039137 36.0 32.5 39.0 22.5 40.5 64-65 32.819573932904035 35.5 32.0 39.0 22.0 40.0 66-67 32.24794880048607 35.0 31.0 38.0 20.0 40.0 68-69 31.65080969748103 35.0 31.0 38.0 18.0 40.0 70-71 31.01788606624597 34.5 30.0 37.0 13.5 39.0 72-73 30.427483161472 34.0 29.5 36.5 8.0 39.0 74-75 29.82341188951974 34.0 29.0 36.0 7.0 38.5 76-77 28.49708286964091 32.5 27.0 34.5 4.5 36.5 78-79 29.17868102263659 34.0 29.0 35.0 2.0 37.0 80-81 28.856794202414925 34.0 29.0 35.0 2.0 36.5 82-83 28.295601559265798 33.5 27.5 35.0 2.0 36.0 84-85 27.709586352253034 33.0 26.0 35.0 2.0 36.0 86-87 27.062691740424587 32.5 25.5 35.0 2.0 35.0 88-89 26.3028959592255 32.0 24.5 34.5 2.0 35.0 90-91 25.745065118943995 32.0 23.0 34.0 2.0 35.0 92-93 24.91608754303092 31.0 19.0 34.0 2.0 35.0 94-95 24.062206535820142 31.0 12.0 34.0 2.0 35.0 96-97 22.98019534896167 30.5 2.0 34.0 2.0 35.0 98-99 21.50401680940785 30.0 2.0 34.0 2.0 35.0 100-101 17.15948730440312 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16254446102090014 1101 2 0.20178943223788792 1101 3 0.1834504629035436 1101 4 0.09837819216294008 1101 5 0.10727432945910209 1101 6 0.13086035464010592 1101 7 0.14297248266802853 1101 8 0.132099129215824 1101 9 0.09831657363621815 1101 10-11 0.16218476703428308 1101 12-13 0.1783016494307006 1101 14-15 0.2114434163039718 1101 16-17 0.147423840324322 1101 18-19 0.23243854885680548 1101 20-21 0.21574585219365616 1101 22-23 0.26481156804138095 1101 24-25 0.3307374458597039 1101 26-27 0.27798551420332984 1101 28-29 0.2699584509188355 1101 30-31 0.3837219144934991 1101 32-33 0.3114675831883247 1101 34-35 0.19242469179648225 1101 36-37 0.24007315125597728 1101 38-39 0.22484944851310473 1101 40-41 0.19100790183978233 1101 42-43 0.254352564103165 1101 44-45 0.37991061367241485 1101 46-47 0.3890149636848719 1101 48-49 0.4069681449751741 1101 50-51 0.5531404378689615 1101 52-53 0.25632268485151144 1101 54-55 0.2920035416797191 1101 56-57 0.3816569160863068 1101 58-59 0.38973170136724633 1101 60-61 0.2617120856960824 1101 62-63 0.3059802049366027 1101 64-65 0.4470011114562098 1101 66-67 0.31543442283048506 1101 68-69 0.27656380404459924 1101 70-71 0.3088708129070419 1101 72-73 0.3528914598878501 1101 74-75 0.3515732702667371 1101 76-77 0.26693757787805694 1101 78-79 0.17206896234605296 1101 80-81 0.24776958420704176 1101 82-83 0.12483010847445897 1101 84-85 0.16765821997335095 1101 86-87 0.17939850559700687 1101 88-89 -0.0012148155079501066 1101 90-91 -0.11475872947336185 1101 92-93 -0.255081058285203 1101 94-95 -0.26376012848623276 1101 96-97 -0.11144272762749807 1101 98-99 -0.22772253297585365 1101 100-101 -0.5903715629960331 1102 1 0.10615222541179747 1102 2 0.07548701979089856 1102 3 0.06771420786006388 1102 4 0.06873943511454428 1102 5 0.10508179865211531 1102 6 0.015224345178793897 1102 7 0.02960721117023013 1102 8 0.05726248660809574 1102 9 0.09221895082172438 1102 10-11 0.12321907520713893 1102 12-13 0.1526216177265738 1102 14-15 0.15247980349999324 1102 16-17 0.12463298771419318 1102 18-19 0.1424046564885728 1102 20-21 0.14024483881613747 1102 22-23 0.13939186280239824 1102 24-25 0.13008313865419296 1102 26-27 0.15792212154957497 1102 28-29 0.07999170748824014 1102 30-31 0.12117444411470757 1102 32-33 0.07172666536005323 1102 34-35 0.13420687491903038 1102 36-37 0.14767461382731994 1102 38-39 0.11930138212166952 1102 40-41 0.17095634752617883 1102 42-43 0.20902829889938346 1102 44-45 0.1057172980634391 1102 46-47 0.1766789256711192 1102 48-49 0.20201692985829567 1102 50-51 0.15419588342741264 1102 52-53 0.07932770025110614 1102 54-55 0.16208731500144324 1102 56-57 0.13284718610187696 1102 58-59 0.31118109646122605 1102 60-61 0.07828392862334255 1102 62-63 0.0693576268230558 1102 64-65 0.08191342972695992 1102 66-67 -0.04277766015569995 1102 68-69 -0.08275858652779178 1102 70-71 -0.034312916571870744 1102 72-73 0.10561827624418285 1102 74-75 -0.022132508559526087 1102 76-77 -0.1333474199220035 1102 78-79 -0.20044362100990654 1102 80-81 -0.2522188114891861 1102 82-83 -0.07913932403413426 1102 84-85 -0.07813653636357643 1102 86-87 -0.2631655540604285 1102 88-89 -0.48331288533192307 1102 90-91 -0.7076119800826355 1102 92-93 -0.6801173534269189 1102 94-95 -0.6810614237995765 1102 96-97 -0.9987558690822063 1102 98-99 -0.9549731243077701 1102 100-101 -1.2054158504800796 1103 1 0.1509474472548007 1103 2 0.1491522623981183 1103 3 0.15470464915937754 1103 4 0.12924207240899221 1103 5 0.1043270073683118 1103 6 0.14770513230975268 1103 7 0.08243663430682346 1103 8 0.09141306299417096 1103 9 0.22643008951239096 1103 10-11 0.17134522380256811 1103 12-13 0.2296744710166294 1103 14-15 0.22347866385465665 1103 16-17 0.24717618683589393 1103 18-19 0.20328991031325216 1103 20-21 0.2394290377230277 1103 22-23 0.26588802022858715 1103 24-25 0.309837577900943 1103 26-27 0.2947451960859624 1103 28-29 0.24140367436096 1103 30-31 0.28243127602000584 1103 32-33 0.2374324399608767 1103 34-35 0.2532468579735152 1103 36-37 0.3704648355509903 1103 38-39 0.2971129050697101 1103 40-41 0.35609277679656515 1103 42-43 0.2836959520653295 1103 44-45 0.26889840023771683 1103 46-47 0.20658939489472772 1103 48-49 0.12552867834870796 1103 50-51 0.04713261819436099 1103 52-53 0.1389987246192277 1103 54-55 0.3225289424865849 1103 56-57 0.32021442637014275 1103 58-59 0.3365352218580142 1103 60-61 0.19048529381105794 1103 62-63 0.23409977460701725 1103 64-65 0.3223957503074004 1103 66-67 0.2440825572719234 1103 68-69 0.19013351710236748 1103 70-71 -0.023635454102599596 1103 72-73 -0.2687127042088093 1103 74-75 -0.0985528885208602 1103 76-77 -0.04498388499276729 1103 78-79 -0.09645098395412788 1103 80-81 -0.02496174688143782 1103 82-83 0.08399926519234313 1103 84-85 -0.1914521882884408 1103 86-87 -0.27557858897773 1103 88-89 -0.31236832221585686 1103 90-91 -0.5160836918180003 1103 92-93 -0.6310954495628636 1103 94-95 -0.626255521510739 1103 96-97 -0.8124290772733325 1103 98-99 -1.1514597648003857 1103 100-101 -1.3361125854856901 1104 1 0.045993723970873646 1104 2 0.06966935042856193 1104 3 0.09814037391302577 1104 4 0.057927840369231376 1104 5 0.001030590494281114 1104 6 0.09821286242523541 1104 7 -0.009567231102195706 1104 8 0.059268765631664166 1104 9 0.1021018992853655 1104 10-11 0.045628977984485175 1104 12-13 0.020819702062986778 1104 14-15 0.08149900116112008 1104 16-17 0.03191050846433541 1104 18-19 0.0770288399420096 1104 20-21 0.1562624127407659 1104 22-23 0.08270323442788907 1104 24-25 0.15910787832374496 1104 26-27 0.16196238963883758 1104 28-29 0.005209321286542945 1104 30-31 0.0381745909419422 1104 32-33 0.15600900765752357 1104 34-35 0.13342532577652833 1104 36-37 0.07369545897640961 1104 38-39 0.20550984224943392 1104 40-41 0.19985441271747106 1104 42-43 0.11013367767046134 1104 44-45 -0.009310145674383818 1104 46-47 0.014251925437925195 1104 48-49 -0.10629390555988749 1104 50-51 -0.24601170797253502 1104 52-53 -0.06823808050992142 1104 54-55 -0.07522301515830776 1104 56-57 -0.15029312459606814 1104 58-59 -0.05382331555327369 1104 60-61 -0.23817860739971053 1104 62-63 -0.38610288888600763 1104 64-65 -0.3853506642482287 1104 66-67 -0.49142312492147866 1104 68-69 -0.5344771669387356 1104 70-71 -0.540786450242809 1104 72-73 -0.7543725074677816 1104 74-75 -0.7847607304816968 1104 76-77 -0.6967957691232343 1104 78-79 -0.6452365802120852 1104 80-81 -0.7112233336241118 1104 82-83 -0.7682239245920748 1104 84-85 -0.7347763662742999 1104 86-87 -0.8456514219389462 1104 88-89 -0.8584241753387474 1104 90-91 -0.995488638726588 1104 92-93 -0.9137604214539898 1104 94-95 -0.8634478414165407 1104 96-97 -1.016008339234535 1104 98-99 -0.9361611545772135 1104 100-101 -1.130464325060256 1105 1 0.16952617667028846 1105 2 0.13899891740187797 1105 3 0.10540970752619927 1105 4 0.044781820615583 1105 5 0.015101313695602414 1105 6 0.06535003351363144 1105 7 0.10209819401878661 1105 8 0.12735355215461652 1105 9 0.21411585635327413 1105 10-11 0.15781794118336023 1105 12-13 0.14274815120108997 1105 14-15 0.10438812540181885 1105 16-17 0.06256725680108133 1105 18-19 0.11578825700711803 1105 20-21 0.11418232013226515 1105 22-23 0.18232251385330756 1105 24-25 0.09686976044230988 1105 26-27 0.16554409406367654 1105 28-29 0.09670504309389827 1105 30-31 0.21529716118988773 1105 32-33 0.2600940685321902 1105 34-35 0.3477997767235834 1105 36-37 0.28554745313343943 1105 38-39 0.3162000687327051 1105 40-41 0.19710250709972854 1105 42-43 0.29092652452513335 1105 44-45 0.2799171742958819 1105 46-47 0.234204038891896 1105 48-49 0.36056225239432393 1105 50-51 0.2039746978012218 1105 52-53 0.2321286922076098 1105 54-55 0.22025082298766563 1105 56-57 0.301438408104012 1105 58-59 0.29130826553664946 1105 60-61 0.272625718475517 1105 62-63 0.21665909932179517 1105 64-65 0.21152197290172836 1105 66-67 0.07625860471805623 1105 68-69 0.11849422827626199 1105 70-71 -0.1136140838424744 1105 72-73 -0.06734802782728977 1105 74-75 -0.044051310891827455 1105 76-77 0.06734522792156383 1105 78-79 -0.11096079061616493 1105 80-81 -0.03923531825449089 1105 82-83 0.08070688499056544 1105 84-85 -0.11875248254284898 1105 86-87 -0.3456963248278129 1105 88-89 -0.2980550374067761 1105 90-91 -0.7230962835338808 1105 92-93 -0.5190243887607409 1105 94-95 -0.445055470104851 1105 96-97 -0.48126435889443187 1105 98-99 -1.0033001842833542 1105 100-101 -1.6710066170846325 1106 1 0.21174990605408084 1106 2 0.21475297239315694 1106 3 0.2325251440246987 1106 4 0.14684542814025292 1106 5 0.1918775853809933 1106 6 0.14630462751911466 1106 7 0.1490614634300158 1106 8 0.15514820927080564 1106 9 0.18084213628925028 1106 10-11 0.22930138495805608 1106 12-13 0.20865300828071298 1106 14-15 0.20128522978646401 1106 16-17 0.31843238635302384 1106 18-19 0.30762789778786725 1106 20-21 0.35406511198632273 1106 22-23 0.40471362845867986 1106 24-25 0.4485739420078332 1106 26-27 0.3948055547978626 1106 28-29 0.3732320866452099 1106 30-31 0.3717530359065009 1106 32-33 0.44703682019184043 1106 34-35 0.5495696956397893 1106 36-37 0.5574957806177139 1106 38-39 0.5013076452608445 1106 40-41 0.46354308171081726 1106 42-43 0.41121571897261333 1106 44-45 0.3876416070283355 1106 46-47 0.38644008890575066 1106 48-49 0.29083486370856093 1106 50-51 0.3356504959424669 1106 52-53 0.3373724264882938 1106 54-55 0.18562769634177556 1106 56-57 0.24953441279134125 1106 58-59 0.2999683462319638 1106 60-61 0.17044494557249124 1106 62-63 0.3261901954587074 1106 64-65 0.3859725786511845 1106 66-67 0.2391101944072247 1106 68-69 0.09414565886998716 1106 70-71 -0.4359495827602764 1106 72-73 -0.3762946870191364 1106 74-75 -0.3583840135220022 1106 76-77 -0.43392176194928567 1106 78-79 -0.4621511374007383 1106 80-81 -0.4300396804061748 1106 82-83 -0.3448320668399454 1106 84-85 -0.463895848416378 1106 86-87 -0.3053518746533044 1106 88-89 -0.3363453067463702 1106 90-91 -0.6527609713678935 1106 92-93 -0.671991140879431 1106 94-95 -0.5103464340613932 1106 96-97 -0.857838161678103 1106 98-99 -1.0588426323643887 1106 100-101 -1.2829116708951709 1107 1 -0.018479857001743483 1107 2 -0.009155894322212532 1107 3 -0.02621380121695438 1107 4 -0.02873364000753753 1107 5 -0.02789918283880155 1107 6 -0.007612595290595436 1107 7 0.02772710084641261 1107 8 -0.013423358084374115 1107 9 -0.04971780080045818 1107 10-11 0.04970984896612407 1107 12-13 -0.004268217971208799 1107 14-15 0.0039342035884999405 1107 16-17 -0.07797961307721124 1107 18-19 0.08068953661948086 1107 20-21 -0.01445308926755473 1107 22-23 0.013899161978571328 1107 24-25 0.07240610726027086 1107 26-27 0.008788786157957418 1107 28-29 -0.007879168876016251 1107 30-31 -0.12268930802718359 1107 32-33 -0.08685787464005301 1107 34-35 0.07671830480628472 1107 36-37 0.0697465172733942 1107 38-39 0.01708461434198938 1107 40-41 -0.012675854524893282 1107 42-43 0.021567856472046287 1107 44-45 -0.04823980994996191 1107 46-47 -0.1791081542467552 1107 48-49 -0.21164094923832266 1107 50-51 -0.13252496103348932 1107 52-53 -0.13777245952289974 1107 54-55 -0.06504052864129761 1107 56-57 -0.2089601511524748 1107 58-59 -0.058386008619123686 1107 60-61 -0.403550140545164 1107 62-63 -0.45404517989928195 1107 64-65 -0.5739657582300381 1107 66-67 -0.4237947528863657 1107 68-69 -0.21577124623746968 1107 70-71 -0.40663876033811164 1107 72-73 -0.7714491697388226 1107 74-75 -0.7372633122664851 1107 76-77 -0.7512034187796566 1107 78-79 -0.7806676648101067 1107 80-81 -0.3820218405057929 1107 82-83 -0.4048603974787177 1107 84-85 -0.7743556042713884 1107 86-87 -0.8943830075047003 1107 88-89 -1.154063147762649 1107 90-91 -1.3086106640697324 1107 92-93 -1.0315038945899495 1107 94-95 -0.9978584053316553 1107 96-97 -1.3225003503184105 1107 98-99 -1.710280431974354 1107 100-101 -1.8002456435714613 1108 1 -0.03893542981099074 1108 2 -0.04074681941591507 1108 3 -0.004043608830187395 1108 4 0.024521691572481075 1108 5 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-0.03387718047676458 2316 52-53 0.002423221750881055 2316 54-55 -0.02694356767970163 2316 56-57 -2.2685421949830697E-4 2316 58-59 0.0525235306228069 2316 60-61 0.4100658435169606 2316 62-63 0.3696047533667297 2316 64-65 0.4343616796153711 2316 66-67 0.39116190426923936 2316 68-69 0.616558386392903 2316 70-71 0.8997684608273246 2316 72-73 1.0544516901706835 2316 74-75 1.37667812229898 2316 76-77 1.152731195611402 2316 78-79 1.09418910663479 2316 80-81 1.256313214994968 2316 82-83 1.3952250748648432 2316 84-85 1.4983704008481489 2316 86-87 1.754019388656431 2316 88-89 1.8501533492313875 2316 90-91 2.0199862195900273 2316 92-93 1.9221414250286664 2316 94-95 2.44692362306845 2316 96-97 2.7864834926199222 2316 98-99 3.301613042185309 2316 100-101 3.4905243462912345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 20.0 5 40.0 6 83.0 7 221.0 8 582.0 9 1133.0 10 1945.0 11 3237.0 12 5051.0 13 7297.0 14 10036.0 15 13946.0 16 18587.0 17 25744.0 18 26314.0 19 21555.0 20 20638.0 21 21627.0 22 23653.0 23 25448.0 24 28691.0 25 32341.0 26 37105.0 27 44349.0 28 53118.0 29 63682.0 30 76784.0 31 93927.0 32 117114.0 33 150711.0 34 203618.0 35 287303.0 36 405022.0 37 477775.0 38 232045.0 39 10443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61255949024051 18.175156426913734 14.918280982446419 23.294003100399337 2 16.07873912862094 19.40845686830292 39.4394229323619 25.07338107071424 3 19.939833621545294 22.831424732600432 29.267826178671474 27.9609154671828 4 13.595520209035925 16.217874302984033 35.26517104191343 34.921434446066606 5 14.475615039802422 36.55947617973876 33.5091573707774 15.45575140968142 6 33.743279122947925 34.89720594896246 16.380936340214603 14.978578587875013 7 29.790224760309712 32.15178994457728 19.227599917519154 18.83038537759386 8 27.318121073699153 34.40836519497118 18.609269021358447 19.66424470997121 9 26.452741150230047 16.76627855545184 19.420225751968758 37.36075454234935 10-11 25.81697751767278 25.915494121665546 26.013833643685885 22.253694716975787 12-13 26.67833190157758 23.055176574245948 26.85576992321251 23.410721600963967 14-15 23.28130140296144 24.55707863541419 25.164745261679933 26.996874699944435 16-17 22.87783837856355 27.76098187833402 26.0315147260303 23.32966501707213 18-19 22.88619217093054 26.093284421980833 28.443260574941874 22.57726283214675 20-21 23.395558626725368 25.087950712971374 28.877052380060412 22.639438280242846 22-23 23.168401807652774 25.02449633951887 28.81145334716409 22.995648505664263 24-25 23.093669271110546 25.263709043170383 28.761891231985448 22.880730453733623 26-27 22.62653432487344 25.611053515299364 28.716169676474223 23.046242483352973 28-29 22.712352131638113 25.99511929625305 28.532656284250425 22.759872287858414 30-31 22.838501463331706 25.508998201186174 28.434894233603387 23.21760610187874 32-33 22.656418473820988 25.45254281842093 28.537763320892996 23.353275386865082 34-35 23.22511938010306 25.6837188306604 28.30399082858558 22.787170960650958 36-37 23.371302690254733 25.275797938903782 28.282599524943606 23.070299845897875 38-39 22.880963071780585 25.596754815496638 28.300524296579578 23.221757816143203 40-41 23.289073635512906 25.4844286300075 28.268378183317864 22.958119551161722 42-43 23.00321808502789 25.440147652155602 28.08078760933963 23.47584665347688 44-45 22.980069813597577 25.695504340378378 28.26014370008132 23.064282145942727 46-47 23.45848394345723 25.980922728570437 27.53515427703894 23.02543905093339 48-49 23.318458353993027 26.066532083520254 27.30825836815967 23.306751194327045 50-51 23.21233984796354 26.313849784010944 27.416973980963373 23.056836387062145 52-53 23.54199593232292 26.06341656539452 27.227678936862855 23.166908565419707 54-55 23.16694913236494 26.178991753890617 27.32384854643801 23.33021056730643 56-57 23.23572386500828 26.40493383540172 27.150991460442437 23.20835083914756 58-59 23.18832222858978 26.048922010693303 27.20659546195943 23.556160298757487 60-61 22.950293247428736 26.24272784352214 27.469001286247153 23.337977622801972 62-63 22.96685669664285 26.278668080690682 27.564455151738304 23.19002007092817 64-65 22.82896465270145 26.336123555649145 27.371126463292356 23.463785328357048 66-67 22.778718612830872 26.333284400290136 27.252195788321167 23.635801198557825 68-69 23.092874039280396 26.005652084833624 27.447683576145206 23.453790299740778 70-71 23.06304501534254 26.12033596352976 27.052262633414557 23.764356387713136 72-73 23.029717141422992 26.245343753068333 27.006773313110926 23.71816579239775 74-75 22.972495630830192 26.719679850693545 26.714622417339008 23.593202101137255 76-77 23.12806423255273 26.22934850182361 27.01781060418061 23.624776661443054 78-79 23.163100733391914 26.37664245643487 27.01051034355175 23.44974646662147 80-81 23.320356436983996 26.44242689476199 26.698239145886184 23.53897752236783 82-83 23.215711961542805 26.398401343419476 26.83672107709743 23.549165617940286 84-85 23.115678364447845 26.580241512609444 26.72858671966003 23.575493403282685 86-87 23.360214762775897 26.46325346317769 26.61690568013297 23.559626093913447 88-89 23.14424428009714 26.61784790745821 26.34719308534296 23.89071472710169 90-91 23.146898605168904 26.545462346173093 26.512674280084475 23.79496476857352 92-93 23.209672842147192 26.276165169471145 26.60797145914866 23.906190529233 94-95 23.103519049868932 26.115242787855582 26.708267894802194 24.07297026747329 96-97 23.218574198164557 25.806794397797912 26.75629649512094 24.218334908916596 98-99 23.052845174897115 26.108846648361183 26.401020720194918 24.43728745654678 100-101 23.580525750735152 25.37694177355473 26.291452575703193 24.751079900006925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 846.0 1 576.0 2 425.5 3 580.0 4 487.0 5 449.5 6 876.5 7 2097.0 8 3487.5 9 4004.5 10 3416.5 11 2596.0 12 2387.5 13 2517.5 14 2717.5 15 2945.0 16 3154.0 17 3357.5 18 3594.0 19 3878.5 20 4244.0 21 4848.0 22 5682.5 23 6735.5 24 8161.5 25 10064.0 26 12701.5 27 15798.5 28 18298.5 29 20397.5 30 23086.5 31 27510.5 32 33722.5 33 36048.0 34 36010.0 35 38680.5 36 42487.5 37 46404.5 38 50828.0 39 54913.5 40 59080.5 41 64632.0 42 68707.5 43 71933.5 44 76049.0 45 82059.5 46 87307.5 47 92724.0 48 98535.0 49 99875.5 50 98371.0 51 97177.5 52 98808.5 53 102876.0 54 105760.0 55 101276.5 56 93061.5 57 85627.0 58 79525.0 59 71779.5 60 59921.0 61 49903.5 62 41285.5 63 30932.5 64 21806.5 65 16104.5 66 11933.5 67 9121.0 68 6904.0 69 5104.5 70 3841.5 71 2608.0 72 1832.0 73 1367.0 74 977.0 75 664.0 76 438.5 77 280.5 78 168.5 79 99.5 80 64.5 81 29.5 82 18.0 83 13.0 84 4.5 85 2.5 86 2.0 87 3.5 88 3.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009168911016702372 2 0.01377304229976751 3 0.009523074961553536 4 2.3610929656744305E-4 5 7.476794391302363E-4 6 0.0018495228231116372 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010624918345534936 14-15 0.0 16-17 0.002616878036955827 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0035613152232255997 26-27 0.019754477812809403 28-29 0.006650411853316313 30-31 0.0030300693059488524 32-33 0.0 34-35 0.010133023977686097 36-37 9.444371862697722E-4 38-39 0.0030300693059488524 40-41 0.002282389866818616 42-43 0.003482612124369785 44-45 1.9675774713953586E-5 46-47 1.7708197242558227E-4 48-49 6.296247908465148E-4 50-51 0.0037383971956511815 52-53 0.007398091292446549 54-55 0.007063603122309337 56-57 0.01469780371132333 58-59 0.021249836691069876 60-61 0.003246502827802342 62-63 0.017963982313839624 64-65 0.018829716401253586 66-67 0.012159628773223317 68-69 0.0282347367145234 70-71 0.015130670755030308 72-73 0.004741861706062814 74-75 0.015012616106746587 76-77 0.01930193499438847 78-79 0.023748660079741978 80-81 0.010624918345534938 82-83 0.03199280968488853 84-85 0.01969545048866754 86-87 0.028274088263951305 88-89 0.01991188401052103 90-91 0.025086612760290825 92-93 0.021918813031344297 94-95 0.012808929338783786 96-97 0.013300823706632625 98-99 0.009641129609837259 100-101 0.008657340874139578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2541196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.96464198884946 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90275987604794 37.66427699070692 2 14.18008992655577 14.453664129532108 3 4.8730726876685235 7.450632147380003 4 2.22518870445522 4.536237827207682 5 1.2454728307470044 3.173753846292999 6 0.7427333456245717 2.271188343176201 7 0.5113254114916628 1.8241661575531607 8 0.37355009798107724 1.523027760680419 9 0.2778328816887581 1.2743688013198133 >10 1.511439424305982 14.049137886029522 >50 0.10705038125843197 3.7338749757577423 >100 0.04353051576845301 4.011427025837315 >500 0.003247590767337583 1.1027848155068465 >1k 0.002474354870349783 1.9271542964795878 >5k 1.5464717939686143E-4 0.5826427064484941 >10k+ 7.732358969843072E-5 0.4216622900912335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10700 0.4210615788786068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9067 0.3568004986628343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5718 0.22501215962877322 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2591 0.1019598645677075 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2560 0.10073996653544237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.935154942790717E-5 0.0 8 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 0.0 9 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 0.0 10-11 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 0.0 12-13 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 7.870309885581434E-5 14-15 3.935154942790717E-5 0.0 0.0 3.935154942790717E-5 7.870309885581434E-5 16-17 5.902732414186076E-5 0.0 0.0 3.935154942790717E-5 1.1805464828372152E-4 18-19 7.870309885581434E-5 0.0 0.0 7.870309885581434E-5 1.574061977116287E-4 20-21 7.870309885581434E-5 0.0 0.0 1.1805464828372152E-4 1.7708197242558227E-4 22-23 7.870309885581434E-5 0.0 0.0 1.574061977116287E-4 1.9675774713953588E-4 24-25 1.1805464828372152E-4 0.0 0.0 3.9351549427907176E-4 1.9675774713953588E-4 26-27 1.1805464828372152E-4 0.0 0.0 0.001003464510411633 1.9675774713953588E-4 28-29 1.377304229976751E-4 0.0 0.0 0.0031284481795186203 1.9675774713953588E-4 30-31 1.574061977116287E-4 0.0 0.0 0.010447836373109354 1.9675774713953588E-4 32-33 1.7708197242558227E-4 0.0 0.0 0.027526408824821068 2.1643352185348946E-4 34-35 1.9675774713953588E-4 0.0 0.0 0.052514642711542124 2.3610929656744305E-4 36-37 2.3610929656744305E-4 0.0 0.0 0.09471917947297256 2.3610929656744305E-4 38-39 2.3610929656744305E-4 0.0 0.0 0.15567472953680078 2.754608459953502E-4 40-41 2.3610929656744305E-4 0.0 0.0 0.22168695370211505 2.754608459953502E-4 42-43 2.754608459953502E-4 0.0 0.0 0.28785658406514103 2.754608459953502E-4 44-45 3.9351549427907176E-4 0.0 0.0 0.36695319841523444 2.951366207093038E-4 46-47 5.312459172767468E-4 0.0 0.0 0.46206589338248605 3.148123954232574E-4 48-49 5.509216919907004E-4 0.0 0.0 0.5621959109017959 3.148123954232574E-4 50-51 5.509216919907004E-4 0.0 0.0 0.6668710323800289 4.3286704370697893E-4 52-53 5.509216919907004E-4 0.0 0.0 0.7658401791912155 5.115701425627933E-4 54-55 5.509216919907004E-4 0.0 0.0 0.8702791913728811 5.115701425627933E-4 56-57 5.509216919907004E-4 0.0 0.0 0.9939217596753656 5.312459172767468E-4 58-59 5.509216919907004E-4 0.0 0.0 1.1247263099737288 5.509216919907004E-4 60-61 5.509216919907004E-4 0.0 0.0 1.2626731664932576 5.509216919907004E-4 62-63 5.509216919907004E-4 0.0 0.0 1.3982195784976836 5.509216919907004E-4 64-65 5.509216919907004E-4 0.0 0.0 1.5406328358772798 5.509216919907004E-4 66-67 5.902732414186076E-4 0.0 0.0 1.7024070555754063 5.509216919907004E-4 68-69 5.902732414186076E-4 0.0 0.0 1.8819878513896606 5.509216919907004E-4 70-71 5.902732414186076E-4 0.0 0.0 2.066212130036408 6.099490161325613E-4 72-73 5.902732414186076E-4 0.0 0.0 2.2564768715203396 6.296247908465148E-4 74-75 6.099490161325613E-4 0.0 0.0 2.4484730811790985 6.296247908465148E-4 76-77 6.296247908465148E-4 0.0 0.0 2.6561508832848784 6.296247908465148E-4 78-79 6.493005655604684E-4 0.0 0.0 2.885196576730012 6.296247908465148E-4 80-81 7.476794391302363E-4 0.0 0.0 3.1258706530310922 6.296247908465148E-4 82-83 7.476794391302363E-4 0.0 0.0 3.376067804293726 6.68976340274422E-4 84-85 7.673552138441899E-4 0.0 0.0 3.6262256040069323 6.68976340274422E-4 86-87 7.870309885581435E-4 0.0 0.0 3.885296529665559 7.083278897023291E-4 88-89 7.870309885581435E-4 0.0 0.0 4.158809473964228 7.673552138441899E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6720 0.0 45.09949 1 GTATCAA 10770 0.0 37.314266 1 TATCAAC 13065 0.0 30.502123 2 ATCAACG 13080 0.0 30.392122 3 TCAACGC 13110 0.0 30.284561 4 CAACGCA 13400 0.0 29.629147 5 AACGCAG 13835 0.0 28.800531 6 ACGCAGA 15605 0.0 25.32129 7 CGCAGAG 15800 0.0 24.888544 8 GAGTACT 9840 0.0 23.795984 12-13 TAAGGTG 1500 0.0 23.429237 5 GTACTTT 10065 0.0 23.027184 14-15 GTAAGGT 1500 0.0 22.479404 4 GCAGAGT 17260 0.0 22.398031 9 GTACATG 9150 0.0 22.064196 1 GTGTAAG 1110 0.0 21.825663 1 TACATGG 8885 0.0 21.811234 2 AGAGTAC 14110 0.0 20.919796 10-11 CAGAGTA 16190 0.0 20.637665 10-11 AGTACTT 10065 0.0 20.267551 12-13 >>END_MODULE