##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938183_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1104529 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4405244226272 33.0 31.0 34.0 31.0 34.0 2 32.640780821508535 34.0 31.0 34.0 31.0 34.0 3 32.71081248206249 34.0 31.0 34.0 31.0 34.0 4 36.10062298047403 37.0 35.0 37.0 35.0 37.0 5 36.05702340092474 37.0 35.0 37.0 35.0 37.0 6 36.147855782872156 37.0 36.0 37.0 35.0 37.0 7 36.14655930265299 37.0 36.0 37.0 35.0 37.0 8 36.1864885394589 37.0 37.0 37.0 35.0 37.0 9 37.82219479977439 39.0 38.0 39.0 35.0 39.0 10-11 37.81366039280091 39.0 38.0 39.0 35.0 39.0 12-13 37.74589938335707 39.0 37.5 39.0 35.0 39.0 14-15 38.78188033089217 40.0 38.0 41.0 35.0 41.0 16-17 38.733461049913586 40.0 38.0 41.0 34.5 41.0 18-19 39.13818378693543 40.0 38.5 41.0 36.0 41.0 20-21 39.15259898110416 40.0 39.0 41.0 35.5 41.0 22-23 39.089549482177475 40.0 39.0 41.0 35.0 41.0 24-25 38.96662016117277 40.0 38.0 41.0 35.0 41.0 26-27 38.59171963796333 40.0 38.0 41.0 34.5 41.0 28-29 38.39795831526379 40.0 38.0 41.0 34.0 41.0 30-31 38.27922218429756 40.0 37.5 41.0 34.0 41.0 32-33 38.10989616388524 40.0 37.0 41.0 34.0 41.0 34-35 38.05700438829582 40.0 37.0 41.0 34.0 41.0 36-37 37.911393906361894 40.0 37.0 41.0 33.5 41.0 38-39 37.60047585894078 40.0 36.5 41.0 33.0 41.0 40-41 37.33514104201882 40.0 36.0 41.0 33.0 41.0 42-43 37.27312184650653 40.0 36.0 41.0 33.0 41.0 44-45 37.105990879370296 40.0 36.0 41.0 32.5 41.0 46-47 36.73181011996969 40.0 36.0 41.0 31.0 41.0 48-49 36.37037959166305 39.0 35.0 41.0 30.5 41.0 50-51 36.068222292035784 39.0 35.0 41.0 30.0 41.0 52-53 35.30215639426398 38.0 35.0 40.0 27.5 41.0 54-55 35.10110282301325 38.0 35.0 40.0 27.0 41.0 56-57 34.96996638386136 38.0 35.0 40.0 27.0 41.0 58-59 34.660698813702496 38.0 34.0 40.0 26.0 41.0 60-61 34.201611275032164 38.0 33.5 40.0 24.0 41.0 62-63 33.59355299860846 37.0 33.0 39.5 22.5 41.0 64-65 32.8277863234012 36.0 32.0 39.0 20.0 40.0 66-67 32.30752655656846 36.0 31.0 39.0 18.0 40.0 68-69 31.886752181246486 35.0 31.0 38.0 15.0 40.0 70-71 31.29193212672551 35.0 30.5 37.5 10.0 39.5 72-73 30.593138342225508 34.5 30.0 37.0 7.0 39.0 74-75 29.986267902427187 34.0 29.0 36.0 2.0 39.0 76-77 28.400544485477518 32.5 27.0 35.0 2.0 37.0 78-79 29.00779653589901 34.0 29.0 35.0 2.0 37.0 80-81 28.90360506605078 34.0 29.0 35.0 2.0 37.0 82-83 28.436175962785946 34.0 28.5 35.0 2.0 36.0 84-85 27.917446712580656 33.5 27.0 35.0 2.0 36.0 86-87 27.226712924694596 33.0 26.0 35.0 2.0 35.5 88-89 26.32177787998323 32.5 24.5 35.0 2.0 35.0 90-91 25.66743697992538 32.0 22.0 34.0 2.0 35.0 92-93 24.99994205674998 31.0 19.5 34.0 2.0 35.0 94-95 24.275007718221975 31.0 12.0 34.0 2.0 35.0 96-97 23.238857920434864 31.0 2.0 34.0 2.0 35.0 98-99 21.926211534509278 30.5 2.0 34.0 2.0 35.0 100-101 18.0668090199533 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02800808175726388 1101 2 0.12499428312052174 1101 3 0.06223282154729759 1101 4 0.023350139939545045 1101 5 -0.03909049173695678 1101 6 0.09082161107178877 1101 7 0.028095619741975497 1101 8 -0.041106607437399134 1101 9 -0.019096371649190758 1101 10-11 -0.02796172868235658 1101 12-13 0.10606070447678917 1101 14-15 0.35576505193142083 1101 16-17 0.22421127364580684 1101 18-19 0.04858818912291696 1101 20-21 0.12632362534950659 1101 22-23 0.050631518463809755 1101 24-25 0.16187255030739323 1101 26-27 0.24142251134973947 1101 28-29 0.26305872727979107 1101 30-31 0.1993443324619406 1101 32-33 0.115186114795506 1101 34-35 0.1510318044070047 1101 36-37 0.13313021607629594 1101 38-39 0.24699113041253185 1101 40-41 0.18152792319053646 1101 42-43 0.2997127579126513 1101 44-45 0.31589111409825676 1101 46-47 0.36864480123067267 1101 48-49 0.60969428814645 1101 50-51 0.731387401544886 1101 52-53 0.8694275253571533 1101 54-55 0.681641077393401 1101 56-57 0.6762610397890896 1101 58-59 0.7717036512883553 1101 60-61 0.5566185676841187 1101 62-63 0.7047513508688397 1101 64-65 0.8915356306450661 1101 66-67 0.8896015245842221 1101 68-69 1.1594513894346683 1101 70-71 1.242941367557055 1101 72-73 1.253605212121382 1101 74-75 1.2766435416210626 1101 76-77 1.2762677062578867 1101 78-79 1.4935801667436728 1101 80-81 1.486254952373553 1101 82-83 1.578704570678024 1101 84-85 1.6286294823478862 1101 86-87 1.914420631727733 1101 88-89 2.1759697356567393 1101 90-91 2.455827927076033 1101 92-93 2.4777148651972 1101 94-95 2.6599549519836074 1101 96-97 2.855722246386893 1101 98-99 3.248594806174303 1101 100-101 3.226736388453503 1102 1 0.24977376330129175 1102 2 0.23867430813950818 1102 3 0.27038046708185703 1102 4 0.252830940100381 1102 5 0.00887001400401033 1102 6 0.07553936561765084 1102 7 0.18423811627953057 1102 8 0.26233517873960466 1102 9 0.21006471042202435 1102 10-11 0.23648352552842766 1102 12-13 0.35681532293722285 1102 14-15 0.4539715591020368 1102 16-17 0.4308008170492812 1102 18-19 0.4157955842442149 1102 20-21 0.37098754439521286 1102 22-23 0.4471559454843188 1102 24-25 0.4539769096426056 1102 26-27 0.3735505493696678 1102 28-29 0.4094533124560442 1102 30-31 0.44769059413374634 1102 32-33 0.3582912336356898 1102 34-35 0.5252736955744126 1102 36-37 0.35498874151970483 1102 38-39 0.4538054537829481 1102 40-41 0.5166490251429181 1102 42-43 0.42779824689982604 1102 44-45 0.34436975860524655 1102 46-47 0.3151729978531108 1102 48-49 0.48823352551409016 1102 50-51 0.5738536426653766 1102 52-53 0.6526419068073608 1102 54-55 0.8160748552605313 1102 56-57 0.8697260858061568 1102 58-59 0.7984461846842308 1102 60-61 0.6168958986942599 1102 62-63 0.8362029547646586 1102 64-65 0.7881696553513109 1102 66-67 0.7560605839149659 1102 68-69 0.9328403732616728 1102 70-71 1.0003297873399255 1102 72-73 1.3212786417331408 1102 74-75 1.27164183831945 1102 76-77 1.2111680770750226 1102 78-79 1.2202149174473327 1102 80-81 1.4146653252011454 1102 82-83 1.4118529142870138 1102 84-85 1.3645737536698697 1102 86-87 1.4465585824045881 1102 88-89 1.6259687583525348 1102 90-91 1.7293097548024328 1102 92-93 1.9889894653401612 1102 94-95 2.1441679987155027 1102 96-97 2.1502070395334734 1102 98-99 2.2223995631327043 1102 100-101 2.674714485670137 1103 1 0.3172079738276068 1103 2 0.29837216389000076 1103 3 0.26313144174072534 1103 4 0.17991427343370958 1103 5 0.11035636877983279 1103 6 0.13048575937983742 1103 7 0.22017866208849313 1103 8 0.150980402911145 1103 9 0.24539609443761634 1103 10-11 0.30796866198067363 1103 12-13 0.37323223326860955 1103 14-15 0.4294832550084706 1103 16-17 0.4060079320590262 1103 18-19 0.30459309886409613 1103 20-21 0.3729977783133407 1103 22-23 0.4504454191685241 1103 24-25 0.43707266987652815 1103 26-27 0.5049772062897375 1103 28-29 0.4743290434501901 1103 30-31 0.4326747559271169 1103 32-33 0.5659240309066433 1103 34-35 0.6784169704282093 1103 36-37 0.7423851840148359 1103 38-39 0.7487006779544885 1103 40-41 0.7385362083787825 1103 42-43 0.7482173502136718 1103 44-45 0.80081469037912 1103 46-47 0.8965874715373232 1103 48-49 1.0574952214205169 1103 50-51 1.0695590520221074 1103 52-53 0.8520205617781258 1103 54-55 0.8414159858648205 1103 56-57 1.0398345166053744 1103 58-59 1.0658377538850132 1103 60-61 1.3377779649710604 1103 62-63 1.3551478865385391 1103 64-65 1.2318526475540423 1103 66-67 1.4083205741683713 1103 68-69 1.2127806754059272 1103 70-71 1.4082245241820281 1103 72-73 1.449720405865694 1103 74-75 1.584720542023156 1103 76-77 1.7017869854570904 1103 78-79 1.693625005166627 1103 80-81 1.7854925669165453 1103 82-83 1.9369199220842823 1103 84-85 2.163154406691312 1103 86-87 2.467026418661895 1103 88-89 2.5586868382745607 1103 90-91 2.7458789555821568 1103 92-93 2.798047701207608 1103 94-95 2.8724513807817793 1103 96-97 3.244901240313201 1103 98-99 3.718409553386117 1103 100-101 3.9609596123757917 1104 1 0.19383888935171 1104 2 0.06235933770689428 1104 3 0.20302713686088225 1104 4 0.060516514330068105 1104 5 0.08224380796603725 1104 6 0.07995502076860106 1104 7 0.11430736620172155 1104 8 0.21364625872715948 1104 9 0.21626997030374895 1104 10-11 0.26644481560001054 1104 12-13 0.1977982438833834 1104 14-15 0.29813005583406493 1104 16-17 0.33473600223446454 1104 18-19 0.2104238522180637 1104 20-21 0.3171826891197682 1104 22-23 0.22315384464398136 1104 24-25 0.34935797873691854 1104 26-27 0.3460596919155705 1104 28-29 0.39025396605081397 1104 30-31 0.30620969621902105 1104 32-33 0.3476070988706752 1104 34-35 0.41109885897310505 1104 36-37 0.5064000004677354 1104 38-39 0.4357537886724643 1104 40-41 0.3318577475207505 1104 42-43 0.4394306776499022 1104 44-45 0.2989488124490052 1104 46-47 0.3696635440715994 1104 48-49 0.4869788569800093 1104 50-51 0.5579952159613626 1104 52-53 0.6352516173582501 1104 54-55 0.5788063367420122 1104 56-57 0.5677351505431645 1104 58-59 0.6176815177063375 1104 60-61 0.6074226649563244 1104 62-63 0.6751482551070183 1104 64-65 0.7513222775112922 1104 66-67 0.9119555808026121 1104 68-69 1.018862976747517 1104 70-71 1.2865692831382454 1104 72-73 1.3793192190751888 1104 74-75 1.1794334271772158 1104 76-77 1.280476355942124 1104 78-79 1.3783469202484504 1104 80-81 1.2386567273175473 1104 82-83 1.3803354461886634 1104 84-85 1.8289226876879212 1104 86-87 1.7416974712934739 1104 88-89 1.8994330051623685 1104 90-91 1.921838155051418 1104 92-93 2.350679280154978 1104 94-95 2.7854738266022103 1104 96-97 3.0457729047684587 1104 98-99 3.330938117443317 1104 100-101 3.265857887475306 1105 1 0.0713463439464519 1105 2 0.12360561040951978 1105 3 0.07518930818102376 1105 4 -0.03312336097489066 1105 5 -0.02021451407245678 1105 6 0.0072896643034354724 1105 7 0.07544181998323296 1105 8 0.06057293262252017 1105 9 0.15925079166455447 1105 10-11 0.111334182637151 1105 12-13 0.1196774794485691 1105 14-15 0.369406146915658 1105 16-17 0.21074406675059265 1105 18-19 0.08907067384994605 1105 20-21 0.20639301034503177 1105 22-23 0.12054304225851098 1105 24-25 0.03383802075371989 1105 26-27 0.15257907386190084 1105 28-29 0.1209228465062182 1105 30-31 0.04579020584223059 1105 32-33 0.08842862909566662 1105 34-35 -0.0992782757517503 1105 36-37 -0.03666299085783464 1105 38-39 -0.19419335147392047 1105 40-41 -0.15744745036484886 1105 42-43 -0.33840545680387635 1105 44-45 -0.21738352693836305 1105 46-47 -0.34762942150172904 1105 48-49 -0.09549183290884855 1105 50-51 -0.10485379461060518 1105 52-53 -0.10284644444711688 1105 54-55 0.00773778835491612 1105 56-57 -0.026573944062420196 1105 58-59 0.032783848961415174 1105 60-61 -0.06252091731529674 1105 62-63 -0.010496567338087459 1105 64-65 -0.03924596591511431 1105 66-67 -0.05446062277559349 1105 68-69 0.28866847959381303 1105 70-71 0.6626281744367013 1105 72-73 0.8539100634774677 1105 74-75 0.6596533377220766 1105 76-77 0.5846746481622382 1105 78-79 0.5053291647591571 1105 80-81 0.6794689392991167 1105 82-83 1.0072456860910748 1105 84-85 1.2232580427977062 1105 86-87 1.469520051938634 1105 88-89 1.3790302876237384 1105 90-91 1.415764354563482 1105 92-93 1.5119696027356255 1105 94-95 1.9383734945339022 1105 96-97 2.3027234672515142 1105 98-99 2.3466901843991366 1105 100-101 2.226107108131309 1106 1 -0.04807779919870825 1106 2 -0.06814716176789659 1106 3 -1.6351439962392078E-4 1106 4 0.0054351067670452835 1106 5 -0.1950507035885849 1106 6 -0.1311301945675325 1106 7 -0.08764411751676704 1106 8 0.07270120883219988 1106 9 0.025241215051650556 1106 10-11 0.09030658687102289 1106 12-13 0.026809535900184756 1106 14-15 -0.06469163534240607 1106 16-17 -0.16672884151553546 1106 18-19 -0.1754045025613422 1106 20-21 -0.27473728199367287 1106 22-23 -0.2598753045156812 1106 24-25 -0.1463377604962801 1106 26-27 -0.30431041706552264 1106 28-29 -0.04101326740507005 1106 30-31 -0.026412415125513178 1106 32-33 -0.007069732799493522 1106 34-35 0.08148029279663405 1106 36-37 0.07017609684378101 1106 38-39 0.04851393179220764 1106 40-41 0.15470602745772766 1106 42-43 0.004856261946123652 1106 44-45 0.008848201892284635 1106 46-47 0.3659542478531108 1106 48-49 0.4443424664863116 1106 50-51 0.2118202225264909 1106 52-53 0.29077124708513935 1106 54-55 0.4811500867420122 1106 56-57 0.4658813230746759 1106 58-59 0.7171021453323831 1106 60-61 0.6507319808701837 1106 62-63 0.556019939718361 1106 64-65 0.46700718428650134 1106 66-67 0.6971774820631111 1106 68-69 0.542070697335749 1106 70-71 0.5691774435899255 1106 72-73 0.8876487227516598 1106 74-75 1.1254106461898203 1106 76-77 1.18641040925095 1106 78-79 1.423990959113997 1106 80-81 1.076756615710405 1106 82-83 1.5798578332684947 1106 84-85 1.7415449631606101 1106 86-87 1.5156232525434739 1106 88-89 1.491980766454386 1106 90-91 1.633317277950578 1106 92-93 1.3091753739049778 1106 94-95 1.6342215288080943 1106 96-97 1.92107654184829 1106 98-99 2.408796409197521 1106 100-101 2.389413559744213 1107 1 0.22565790964275578 1107 2 0.23955958365893082 1107 3 0.17081971730769396 1107 4 0.1877598018889941 1107 5 0.026521323850438705 1107 6 0.14395174140807399 1107 7 0.21275889315396768 1107 8 0.09486679572244583 1107 9 0.2341455595638422 1107 10-11 0.21720111812102516 1107 12-13 0.22357401760750406 1107 14-15 0.439923456121015 1107 16-17 0.3526628315027551 1107 18-19 0.2348284433228045 1107 20-21 0.13215963111608886 1107 22-23 0.29096692109407485 1107 24-25 0.313792289046404 1107 26-27 0.3002403641844751 1107 28-29 0.22785439646688133 1107 30-31 0.2654892617037561 1107 32-33 0.355712755767577 1107 34-35 0.1827703080405385 1107 36-37 0.3228280594962172 1107 38-39 0.19806471304220707 1107 40-41 0.43562542532358606 1107 42-43 0.3586677139278365 1107 44-45 0.30476045417886866 1107 46-47 0.3199443393165211 1107 48-49 0.48249843066314213 1107 50-51 0.43098609523990916 1107 52-53 0.5471879772680666 1107 54-55 0.40685511722981715 1107 56-57 0.3327110677393037 1107 58-59 0.39276811332018724 1107 60-61 0.30202914169336026 1107 62-63 0.40018104871226257 1107 64-65 0.4773639868779611 1107 66-67 0.5385532442582388 1107 68-69 0.6757101790430688 1107 70-71 0.6545980800228541 1107 72-73 0.769050209032148 1107 74-75 0.8959603794215276 1107 76-77 0.7574598566594872 1107 78-79 0.7200675597237556 1107 80-81 0.9513483649482097 1107 82-83 1.2251484201587388 1107 84-85 1.2295980309959766 1107 86-87 1.544563324952012 1107 88-89 1.6061895126434074 1107 90-91 1.7616957078286255 1107 92-93 2.0956183045452192 1107 94-95 2.420068518137363 1107 96-97 2.7325370982507273 1107 98-99 2.630796695020692 1107 100-101 2.616684901207627 1108 1 0.16204495790630347 1108 2 0.2069894440847051 1108 3 0.12084744417455795 1108 4 0.16185068961868154 1108 5 0.040554878058813415 1108 6 0.08026265827447077 1108 7 0.10578864079248262 1108 8 0.020751470754163392 1108 9 0.16086486028286373 1108 10-11 0.11914716821736704 1108 12-13 0.12755164813525255 1108 14-15 0.28331083334737883 1108 16-17 0.32823311206105643 1108 18-19 0.2647137762801819 1108 20-21 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74-75 0.8155406478432567 2316 76-77 1.2144749553819025 2316 78-79 1.1105092296166426 2316 80-81 0.974767838428658 2316 82-83 1.1349845280436277 2316 84-85 1.3600267959979107 2316 86-87 1.506909111505113 2316 88-89 1.4686440229027973 2316 90-91 1.7513004955431732 2316 92-93 1.7759438304195285 2316 94-95 2.4193829458054488 2316 96-97 2.7316389178403533 2316 98-99 3.249763981122122 2316 100-101 3.9867184539241087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 6.0 5 26.0 6 78.0 7 186.0 8 364.0 9 566.0 10 965.0 11 1687.0 12 2607.0 13 4127.0 14 4932.0 15 6552.0 16 8458.0 17 11299.0 18 11978.0 19 9559.0 20 8689.0 21 8883.0 22 9909.0 23 11091.0 24 12615.0 25 14547.0 26 16338.0 27 18486.0 28 22226.0 29 26958.0 30 32300.0 31 38907.0 32 48321.0 33 61680.0 34 83972.0 35 127522.0 36 162042.0 37 209770.0 38 121039.0 39 5833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.75136823025923 17.703111461989682 13.903573378335924 19.641946929415163 2 13.47130741550325 22.818604769088395 40.57890438099101 23.131183434417338 3 23.280042444391327 28.263093660140658 30.62415346617124 17.832710429296768 4 11.793965490704508 16.539763189964095 39.24024503084615 32.426026288485254 5 12.217885288609537 41.01157918972866 33.84687622939377 12.923659292268031 6 26.13666096589587 37.411874201582755 18.805934475237862 17.645530357283512 7 24.339967533672723 38.05866572991746 19.503607419995312 18.097759316414507 8 21.392253328577144 32.60719983051553 21.35884533274907 24.64170150815825 9 24.771282601000063 21.509711379239477 21.685895073827847 32.033110945932606 10-11 22.535125831915686 25.58538526376401 27.55228699291734 24.32720191140296 12-13 31.178448008155513 23.869223895434164 25.300195830077797 19.65213226633253 14-15 26.27984416887198 25.58782974462418 29.209554479782785 18.922771606721057 16-17 18.975192140722424 29.399137550938004 31.426019597493593 20.199650710845983 18-19 17.712934653594427 32.71407993814558 31.61863563564198 17.954349772618013 20-21 20.096846710226714 30.077843134947113 27.946120020388783 21.879190134437394 22-23 18.840994997849755 29.244381068785223 26.448020642357573 25.466603291007445 24-25 20.657233639664526 29.18932915415561 29.594798118826805 20.558639087353054 26-27 23.86830232446542 26.0833486498942 30.607558926506766 19.44079009913361 28-29 19.629679256950247 26.863758217303484 33.83365217210232 19.672910353643953 30-31 18.861704853380942 32.5746087246238 31.02521527275427 17.53847114924099 32-33 18.16443932210019 31.40917984045688 28.90431124941038 21.522069588032547 34-35 19.86744982850603 27.735799683451468 29.30201772526919 23.09473276277331 36-37 22.32077662519324 27.904139571624842 31.007697896602377 18.76738590657954 38-39 24.749095594250374 24.380044628366313 32.414282344813664 18.456577432569652 40-41 19.845891961391498 26.883069846616326 35.08742145198384 18.18361674000833 42-43 18.08349012101776 30.82122944481768 31.937379692991907 19.157900741172657 44-45 18.649421905132684 32.312288929732276 28.533123885232097 20.505165279902943 46-47 18.42242258917602 29.04830927933988 26.167398049304275 26.361870082179824 48-49 20.684020066471774 27.936930583081114 28.632747533111395 22.746301817335716 50-51 24.26350960454637 28.919747693360698 28.77570439526712 18.041038306825804 52-53 19.40578291742453 28.621385223928026 32.899090924729 19.07374093391844 54-55 17.082801809640127 28.225062447432343 33.48997627042839 21.202159472499137 56-57 20.138185660216102 32.22949883140181 27.33786407503292 20.294451433349163 58-59 18.21061284945891 29.530279422269583 29.03373293050703 23.225374797764477 60-61 19.173693040200845 26.700159072328567 30.301332060996135 23.824815826474453 62-63 25.059278660859064 26.049655554539537 28.3312615603574 20.559804224244 64-65 22.86055866346651 26.84261798467944 31.27482392947582 19.021999422378226 66-67 18.470604638361362 28.093402792867177 32.56920603189779 20.86678653687367 68-69 17.9489489339954 32.11499278194223 30.337299907697584 19.59875837636479 70-71 18.61671354939526 30.441663369635386 27.18511691408736 23.75650616688199 72-73 18.154805421498857 28.95511523695405 26.073025729983428 26.81705361156367 74-75 20.087883613739432 28.52238374909124 28.3136069763673 23.076125660802024 76-77 22.426255897310075 25.604126283691965 29.833259244438125 22.136358574559836 78-79 19.268031894137682 25.893887801949973 32.30852245617815 22.529557847734193 80-81 18.63221213043037 30.82026892935263 29.66606550490345 20.881453435313546 82-83 18.526453978565204 29.946197983273166 27.809599257591017 23.717748780570613 84-85 19.395131773667178 26.677371471047458 28.111247876279382 25.816248879005983 86-87 21.312106212862123 26.65652028657337 29.596993750107515 22.434379750456994 88-89 23.538404152358154 23.980040361095092 30.359094238358615 22.12246124818814 90-91 19.37986952579403 25.9000035762481 32.77821512626193 21.941911771695942 92-93 18.008354692362083 28.84745443532945 30.590188215972603 22.554002656335868 94-95 18.27500072429128 29.939032781423375 27.974256877145713 23.811709617139627 96-97 17.91041060072719 27.684087682702476 26.202414062839512 28.203087653730822 98-99 19.835739939829555 26.60450744163349 27.719869736331056 25.8398828822059 100-101 22.197470596064022 26.6572448527834 28.41206523323516 22.73321931791741 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5936.0 1 3523.0 2 1543.0 3 1988.0 4 1470.0 5 1465.0 6 3427.0 7 6514.0 8 8367.5 9 8068.5 10 6019.0 11 4008.0 12 3475.0 13 3272.5 14 2851.5 15 2299.0 16 1906.5 17 1784.5 18 1876.0 19 1991.5 20 2103.0 21 2423.5 22 2825.5 23 3216.0 24 3882.0 25 4768.5 26 5837.5 27 7154.0 28 8587.5 29 10016.5 30 11462.0 31 13170.5 32 15017.5 33 17105.0 34 19110.5 35 20444.5 36 21664.0 37 22800.5 38 23479.5 39 24196.0 40 25203.0 41 25807.0 42 25974.5 43 26186.5 44 27298.0 45 28661.5 46 35271.0 47 52871.5 48 147389.0 49 152211.0 50 61590.0 51 46812.0 52 35155.0 53 22496.0 54 17316.5 55 14633.5 56 12376.5 57 9916.5 58 7710.0 59 5834.5 60 4271.5 61 3074.5 62 2270.5 63 1656.0 64 1159.5 65 837.0 66 645.0 67 549.0 68 386.5 69 228.5 70 185.5 71 166.0 72 117.0 73 73.0 74 45.0 75 23.0 76 17.5 77 12.5 78 5.5 79 1.5 80 2.0 81 3.5 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015119566801777045 3 0.0022634082038588393 4 0.0013580449223153036 5 0.12159028871129685 6 0.0 7 0.0 8 2.716089844630607E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.621453126174143E-4 24-25 4.979498048489447E-4 26-27 0.005432179689261215 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010592750394059368 36-37 5.884861330032982E-4 38-39 0.006744956447499341 40-41 0.009868459768824538 42-43 0.005703788673724276 44-45 0.00353091679801979 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.5268164077176787E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.053632815435357E-5 68-69 6.790224611576518E-4 70-71 0.0 72-73 4.5268164077176787E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.053632815435357E-5 82-83 0.001493849414546834 84-85 0.00366672129025132 86-87 0.0027160898446306073 88-89 0.0 90-91 0.001765458399009895 92-93 0.0 94-95 9.053632815435357E-5 96-97 9.053632815435357E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1104529.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.02059442574139 #Duplication Level Percentage of deduplicated Percentage of total 1 71.24770634561047 27.08880146730777 2 15.54093758535499 11.817513698570858 3 5.917840138818297 6.7499939938315086 4 2.9270837953187656 4.451578633278984 5 1.536884984736553 2.921664034184012 6 0.8922301784005868 2.0353873048425357 7 0.5226824767717022 1.391088892394522 8 0.3374827367820081 1.0265035408702372 9 0.23845947736426273 0.8159733968256804 >10 0.7262048610315696 4.336620370904363 >50 0.04827725903833584 1.3304381540952264 >100 0.04566055877902846 3.5672942029567984 >500 0.0066588314848262 1.770400741985869 >1k 0.007372277715343292 6.206167830869565 >5k 0.002378154101723643 6.987386544391419 >10k+ 0.0021403386915512784 17.503187192690632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 44963 4.0707849228042 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35576 3.2209204104192826 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28927 2.618944364520986 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 17782 1.6099169872407153 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15875 1.437264209450363 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14809 1.340752483637822 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13135 1.1891946703074343 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11907 1.078016059333888 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10605 0.9601377600769196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9486 0.858827608872198 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9254 0.837823180740388 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8854 0.8016086494786465 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8031 0.7270972514076135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7345 0.6649893302937271 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7162 0.6484211822414803 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7031 0.63656092325326 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6827 0.6180915123097718 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6645 0.6016139005856795 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6643 0.6014328279293708 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4850 0.4391011915486148 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4577 0.4143847739624763 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4018 0.36377496652419267 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3662 0.33154403370124275 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 3035 0.2747777559484631 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3004 0.27197112977567817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2775 0.25123831062833113 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2582 0.23376479929454094 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2568 0.23249729070038 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2566 0.2323162180440713 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2461 0.22280990358786415 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2168 0.19628275943863854 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2167 0.1961922231104842 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2047 0.18532786373196178 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2026 0.18342660084072035 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1927 0.17446350435343932 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1904 0.1723811688058892 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1823 0.16504772622538658 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1778 0.16097359145844067 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1757 0.15907232856719924 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1661 0.1503808410643813 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1568 0.14196096254602641 RNA PCR Primer, Index 31 (95% over 22bp) GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1564 0.141598817233409 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1520 0.13761521879461744 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1457 0.13191143012089315 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1454 0.13163982113643008 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.11398523714633114 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1221 0.11054485667646571 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1113 0.10076693323579552 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.1687714854023753E-4 0.0 12-13 0.0 0.0 0.0 5.432179689261214E-4 0.0 14-15 0.0 0.0 0.0 6.337542970804751E-4 0.0 16-17 0.0 0.0 0.0 8.148269533891821E-4 0.0 18-19 0.0 0.0 0.0 0.0011769722660065966 0.0 20-21 0.0 0.0 0.0 0.0018559947271642482 0.0 22-23 0.0 0.0 0.0 0.0046173527358720325 0.0 24-25 0.0 0.0 0.0 0.011498113675602904 0.0 26-27 0.0 0.0 0.0 0.022317204890048157 0.0 28-29 0.0 0.0 0.0 0.04599245470241162 0.0 30-31 0.0 0.0 0.0 0.10094800589210423 0.0 32-33 0.0 0.0 0.0 0.19162013853868934 0.0 34-35 0.0 0.0 0.0 0.39229390989281404 0.0 36-37 0.0 0.0 0.0 0.6636765535354889 0.0 38-39 0.0 0.0 0.0 0.9172235405317561 0.0 40-41 0.0 0.0 0.0 1.1700915050668654 0.0 42-43 0.0 0.0 0.0 1.4239101010475959 0.0 44-45 0.0 0.0 0.0 1.6942515769164956 0.0 46-47 0.0 0.0 0.0 2.0555820625805206 0.0 48-49 0.0 0.0 0.0 2.4805595869370567 0.0 50-51 0.0 0.0 0.0 2.816132487241168 0.0 52-53 0.0 0.0 0.0 3.0886921031498495 0.0 54-55 0.0 0.0 0.0 3.3735193915234456 0.0 56-57 0.0 0.0 0.0 3.6653632453290044 0.0 58-59 0.0 0.0 0.0 4.11777327711631 0.0 60-61 0.0 0.0 0.0 4.744873154077439 0.0 62-63 0.0 0.0 0.0 5.203982874148166 0.0 64-65 0.0 0.0 0.0 5.536884952771724 0.0 66-67 0.0 0.0 0.0 5.9236108784830455 0.0 68-69 0.0 0.0 0.0 6.329983187403862 0.0 70-71 0.0 0.0 0.0 6.783389118800865 0.0 72-73 0.0 0.0 0.0 7.316557555301853 0.0 74-75 0.0 0.0 0.0 7.842935767191264 0.0 76-77 0.0 0.0 0.0 8.282399104052496 0.0 78-79 0.0 0.0 0.0 8.749973970805655 0.0 80-81 0.0 0.0 0.0 9.239141751823627 0.0 82-83 0.0 0.0 0.0 9.724552275223196 4.5268164077176787E-5 84-85 0.0 0.0 0.0 10.508642145203975 9.053632815435357E-5 86-87 0.0 0.0 0.0 11.373807297046977 9.053632815435357E-5 88-89 0.0 0.0 0.0 11.93861818023791 9.053632815435357E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 455 0.0 24.039997 5 TATACTG 300 0.0 23.778694 5 GTGTAGC 645 0.0 23.595432 1 ACCACTC 1105 0.0 23.425112 94-95 GAGTACT 6715 0.0 22.774944 12-13 CATGGGG 3025 0.0 21.6956 4 AGTACTT 6710 0.0 21.376268 12-13 GACGCTC 100 5.3962503E-7 21.372728 94-95 CTCCGAC 190 0.0 21.247742 94-95 GTATAGC 430 0.0 21.01468 1 GTACTTT 7305 0.0 20.805454 14-15 GAGAGTA 445 0.0 20.278744 9 ACTTTTT 7775 0.0 19.792109 16-17 GTCTAGC 145 6.0941925E-4 19.679811 1 ATGGGGG 1115 0.0 19.620089 5 TAGCCCT 945 0.0 19.123606 4 GTCTAGA 250 2.1434062E-7 19.023817 1 GTACATG 9500 0.0 18.923693 1 ATGGGGA 2090 0.0 18.886427 5 TACTTTT 7605 0.0 18.798134 14-15 >>END_MODULE