##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1096246 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04956369282077 33.0 31.0 34.0 31.0 34.0 2 32.18902600328758 34.0 31.0 34.0 31.0 34.0 3 32.26284520080347 34.0 31.0 34.0 30.0 34.0 4 35.70727282015168 37.0 35.0 37.0 35.0 37.0 5 35.738293229804256 37.0 35.0 37.0 35.0 37.0 6 35.80017167679517 37.0 36.0 37.0 35.0 37.0 7 35.78395633826714 37.0 36.0 37.0 35.0 37.0 8 35.79485626401373 37.0 36.0 37.0 35.0 37.0 9 37.41757780644126 39.0 38.0 39.0 35.0 39.0 10-11 37.35800723560223 39.0 37.5 39.0 35.0 39.0 12-13 37.28976616562341 39.0 37.0 39.0 34.5 39.0 14-15 38.34407423151373 40.0 38.0 41.0 34.0 41.0 16-17 38.246791322385675 40.0 38.0 41.0 34.0 41.0 18-19 38.5813476172319 40.0 38.0 41.0 35.0 41.0 20-21 38.577909064206395 40.0 38.0 41.0 34.5 41.0 22-23 38.53412418380546 40.0 38.0 41.0 34.5 41.0 24-25 38.430758242219355 40.0 38.0 41.0 34.0 41.0 26-27 38.09319213023355 40.0 37.5 41.0 33.5 41.0 28-29 37.85716344689057 40.0 37.5 41.0 33.5 41.0 30-31 37.739606347480404 40.0 37.0 41.0 33.5 41.0 32-33 37.554322205052515 40.0 37.0 41.0 33.0 41.0 34-35 37.392924580796645 40.0 36.0 41.0 33.0 41.0 36-37 37.062930218217446 39.0 35.5 41.0 32.5 41.0 38-39 36.657195100369805 39.0 35.0 40.5 31.0 41.0 40-41 36.40180306245131 39.0 35.0 40.0 30.5 41.0 42-43 36.45527144454803 39.0 35.0 41.0 31.0 41.0 44-45 36.30481525132133 39.0 35.0 41.0 30.0 41.0 46-47 35.90048583985711 39.0 35.0 41.0 29.5 41.0 48-49 35.548216367494156 39.0 35.0 40.5 27.5 41.0 50-51 34.83669860596983 38.5 34.5 39.5 26.5 40.5 52-53 34.75518040658757 38.0 34.5 40.0 26.0 40.5 54-55 34.93868255847684 38.0 35.0 40.0 26.0 41.0 56-57 34.84986900750379 38.0 35.0 40.0 25.5 41.0 58-59 34.66715180716737 38.0 35.0 40.0 24.5 41.0 60-61 34.24863671110317 38.0 34.0 40.0 22.5 41.0 62-63 33.678054013423996 37.5 33.5 40.0 21.5 41.0 64-65 32.971455768139634 36.5 32.0 39.5 18.0 41.0 66-67 32.47298507816676 36.0 32.0 39.0 13.0 40.5 68-69 32.040907788945184 36.0 31.5 39.0 10.5 40.0 70-71 31.479969368189256 35.0 31.0 37.5 7.0 40.0 72-73 30.729015202792073 35.0 30.5 37.0 2.0 39.0 74-75 30.077213964748786 34.5 29.5 37.0 2.0 39.0 76-77 29.29929139992301 34.0 28.5 36.0 2.0 38.5 78-79 28.620920851706643 34.0 27.5 35.5 2.0 37.0 80-81 28.11137874163281 34.0 26.5 35.0 2.0 37.0 82-83 27.47108860602456 33.0 26.0 35.0 2.0 36.0 84-85 26.89540668791494 33.0 25.5 35.0 2.0 36.0 86-87 26.100066955774523 32.0 23.5 35.0 2.0 35.0 88-89 25.162643694937085 31.5 19.0 34.5 2.0 35.0 90-91 24.26550290719419 31.0 14.0 34.0 2.0 35.0 92-93 23.550246021422197 31.0 4.5 34.0 2.0 35.0 94-95 22.724696372894407 30.5 2.0 34.0 2.0 35.0 96-97 21.426194941646308 29.5 2.0 34.0 2.0 35.0 98-99 19.93227706190034 29.0 2.0 34.0 2.0 35.0 100-101 16.23943348482001 13.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35944934333412704 1101 2 0.29550405818831393 1101 3 0.21147386665515455 1101 4 0.1335423143267107 1101 5 0.19649056743899962 1101 6 0.24693460634713205 1101 7 0.3012233167146121 1101 8 0.2859938148961234 1101 9 0.31783311011807314 1101 10-11 0.347203200560827 1101 12-13 0.3980037435102801 1101 14-15 0.6128036737379361 1101 16-17 0.47086360828594565 1101 18-19 0.46863269474239644 1101 20-21 0.4826734152136751 1101 22-23 0.4843287869727746 1101 24-25 0.5090022710649578 1101 26-27 0.5442701946433246 1101 28-29 0.4517753865912013 1101 30-31 0.3797747280550894 1101 32-33 0.41416872088553447 1101 34-35 0.5837402015872399 1101 36-37 0.6604353500894788 1101 38-39 0.6680227515474684 1101 40-41 0.757970447888944 1101 42-43 0.9284852018746719 1101 44-45 0.6607412810780104 1101 46-47 0.757335411153214 1101 48-49 0.9158026175975849 1101 50-51 1.042588291856191 1101 52-53 0.8482395177231155 1101 54-55 0.818865417802094 1101 56-57 0.9619897825260679 1101 58-59 0.8848588699217856 1101 60-61 0.9171067128925827 1101 62-63 0.9288802697114633 1101 64-65 0.9422208301749251 1101 66-67 0.8225491765067332 1101 68-69 1.1272338355696263 1101 70-71 1.3752921179503126 1101 72-73 1.5641362364895457 1101 74-75 1.6713937464452506 1101 76-77 1.4849700074443994 1101 78-79 1.6323455855889755 1101 80-81 2.0356612273319747 1101 82-83 1.8355689159798771 1101 84-85 2.0920124581636124 1101 86-87 2.2149745913961425 1101 88-89 2.7292486576928106 1101 90-91 2.8972965639298813 1101 92-93 3.1836172421835656 1101 94-95 3.611553507143597 1101 96-97 3.754760969376914 1101 98-99 4.1273413779917725 1101 100-101 4.063175013106797 1102 1 0.29923361082758504 1102 2 0.3035541111003326 1102 3 0.20149917778400805 1102 4 0.02787299277635924 1102 5 0.2652402966903651 1102 6 0.22570172539607825 1102 7 0.13012874963393273 1102 8 0.18476538969391498 1102 9 0.3813733263456669 1102 10-11 0.351077845493343 1102 12-13 0.38114120955066966 1102 14-15 0.49020126887126736 1102 16-17 0.28824474190270877 1102 18-19 0.474120073214948 1102 20-21 0.419149908044254 1102 22-23 0.5139236232549464 1102 24-25 0.5063572890426684 1102 26-27 0.47918593767038686 1102 28-29 0.5966998554597396 1102 30-31 0.41493615612258594 1102 32-33 0.45680342993132683 1102 34-35 0.35060861465991167 1102 36-37 0.5006367251830781 1102 38-39 0.44447102463536226 1102 40-41 0.5417137720013514 1102 42-43 0.5876247472645204 1102 44-45 0.5785797478053212 1102 46-47 0.5321257743586258 1102 48-49 0.7070117964402058 1102 50-51 0.6397694118581683 1102 52-53 0.6058962889142236 1102 54-55 0.7052809535130748 1102 56-57 0.8011409623712353 1102 58-59 0.7127623782051487 1102 60-61 0.7219634791328602 1102 62-63 0.4362916652891329 1102 64-65 0.5512492912706648 1102 66-67 0.6153989373196609 1102 68-69 0.8541861652455296 1102 70-71 0.9422644984960193 1102 72-73 1.0943307423036757 1102 74-75 1.1690866587103166 1102 76-77 0.9745806863891282 1102 78-79 1.42968575107054 1102 80-81 1.5319252056968367 1102 82-83 1.6186655376558576 1102 84-85 1.4139922959000906 1102 86-87 1.5864806963909146 1102 88-89 2.249023518605373 1102 90-91 2.2364087559726578 1102 92-93 2.615078542148293 1102 94-95 2.913842648112354 1102 96-97 3.221076745822092 1102 98-99 2.913230887797514 1102 100-101 2.977359146927789 1103 1 0.5645847543208049 1103 2 0.44235192530798173 1103 3 0.4643002302025465 1103 4 0.2658410154438329 1103 5 0.31863029871424686 1103 6 0.4932791388751312 1103 7 0.42480594048598874 1103 8 0.38755834658523014 1103 9 0.5643929579991749 1103 10-11 0.556643520457925 1103 12-13 0.5769517742136898 1103 14-15 0.8225732583470844 1103 16-17 0.7705560149243595 1103 18-19 0.7654679784972771 1103 20-21 0.802139181470686 1103 22-23 0.8076394702494838 1103 24-25 0.8610729336490266 1103 26-27 0.8915619749098127 1103 28-29 0.8848297886716452 1103 30-31 0.8441610093911649 1103 32-33 1.001349068466034 1103 34-35 0.937888517513592 1103 36-37 1.0010313821351176 1103 38-39 1.145786547808811 1103 40-41 1.2065184674071432 1103 42-43 1.3072766395940079 1103 44-45 1.2381324699263558 1103 46-47 1.6068677294284512 1103 48-49 1.5543706306842822 1103 50-51 1.4013763740115763 1103 52-53 1.4100958436602227 1103 54-55 1.4425102593216081 1103 56-57 1.4240614238162905 1103 58-59 1.5373930197756778 1103 60-61 1.3464119713409204 1103 62-63 1.173824553368469 1103 64-65 1.341312031606627 1103 66-67 1.515550728455061 1103 68-69 1.7361736171783377 1103 70-71 1.6555614019568559 1103 72-73 1.9311755105692043 1103 74-75 2.122092123191191 1103 76-77 2.251943568396822 1103 78-79 2.117633534919964 1103 80-81 2.0561105932702013 1103 82-83 2.252160781533618 1103 84-85 2.1922962829472503 1103 86-87 2.3575189477570326 1103 88-89 2.7892076918496542 1103 90-91 3.2518104965110126 1103 92-93 3.4087539115067216 1103 94-95 3.5928651132003893 1103 96-97 3.954025541612417 1103 98-99 3.8969791169009333 1103 100-101 3.8881970339951835 1104 1 0.12290574197512427 1104 2 0.12968525864131664 1104 3 0.15451010674576082 1104 4 0.13331015124630596 1104 5 0.19580313822041973 1104 6 0.04605691665290834 1104 7 0.12423803925141641 1104 8 0.2287435317704123 1104 9 0.1919361678757241 1104 10-11 0.2121188290999001 1104 12-13 0.21984066310258044 1104 14-15 0.35064115958164876 1104 16-17 0.43710539764041556 1104 18-19 0.3439124229417203 1104 20-21 0.2664231320879651 1104 22-23 0.3243061369161566 1104 24-25 0.3776223163650698 1104 26-27 0.4391422218233956 1104 28-29 0.41995941830128203 1104 30-31 0.28229681186029865 1104 32-33 0.27837993266356165 1104 34-35 0.40775888788395775 1104 36-37 0.3550684191721487 1104 38-39 0.4069655601544895 1104 40-41 0.5150061217281277 1104 42-43 0.3168815778656153 1104 44-45 0.1894349390621528 1104 46-47 -0.010002640941920049 1104 48-49 0.25231507512872753 1104 50-51 0.09411094191281677 1104 52-53 0.1272378189688652 1104 54-55 0.3850596420376604 1104 56-57 0.3599688312236964 1104 58-59 0.4468498098991347 1104 60-61 0.18997987257548488 1104 62-63 0.3617998620104501 1104 64-65 0.37600338963131463 1104 66-67 0.5728470247513542 1104 68-69 0.49840201224006364 1104 70-71 0.7512836241790772 1104 72-73 0.8113853871124199 1104 74-75 1.1814254565245257 1104 76-77 0.8284003585202804 1104 78-79 1.0345409423273715 1104 80-81 1.1297093587023 1104 82-83 1.5059879420274456 1104 84-85 1.6811234434410771 1104 86-87 1.5453522810903664 1104 88-89 1.9154360869113845 1104 90-91 2.165285805152987 1104 92-93 2.44746378804993 1104 94-95 2.590383631718911 1104 96-97 2.8268527021062475 1104 98-99 2.963244548999697 1104 100-101 3.376252589550738 1105 1 0.15198844874203843 1105 2 0.14410631127289975 1105 3 0.1238634902044069 1105 4 0.010791354253825602 1105 5 0.12799862694222952 1105 6 0.14286142793110201 1105 7 0.20527563323638276 1105 8 0.013908945304251574 1105 9 0.058793310732866644 1105 10-11 0.016381986994637998 1105 12-13 0.10146096385445702 1105 14-15 0.23731033251397804 1105 16-17 0.19506404425695223 1105 18-19 0.1808710695582647 1105 20-21 0.1203629817120273 1105 22-23 0.1169753098484776 1105 24-25 0.21039299305680714 1105 26-27 0.2771347030263982 1105 28-29 0.2110759596546572 1105 30-31 0.2184659847926227 1105 32-33 0.2931468499568055 1105 34-35 0.1282776848764442 1105 36-37 0.14229774248041593 1105 38-39 0.1486084172973534 1105 40-41 0.2610587533070756 1105 42-43 0.34824248012125025 1105 44-45 0.28652892402455876 1105 46-47 0.1972116447723664 1105 48-49 0.614510563850537 1105 50-51 0.5062763554466443 1105 52-53 0.4821776685929251 1105 54-55 0.5912664089549509 1105 56-57 0.6679349966372286 1105 58-59 0.6856618399743226 1105 60-61 0.8036302485153399 1105 62-63 0.5454051251683367 1105 64-65 0.6525635400072574 1105 66-67 0.918613942044594 1105 68-69 1.0182027641197635 1105 70-71 1.1098324963595267 1105 72-73 1.3089718532778392 1105 74-75 1.408057035471895 1105 76-77 1.3894981028811806 1105 78-79 1.5898905663875205 1105 80-81 1.810295824867712 1105 82-83 2.0437323029296977 1105 84-85 1.9494693081027314 1105 86-87 2.1512846119174327 1105 88-89 2.30666916961815 1105 90-91 2.4636166322206563 1105 92-93 2.573110404591283 1105 94-95 2.99456032344823 1105 96-97 3.137702326166398 1105 98-99 3.5827746241876675 1105 100-101 3.4241097324078815 1106 1 0.0549351537398266 1106 2 0.08083231746484643 1106 3 -0.027784010901299894 1106 4 0.08081015124630397 1106 5 -0.020601273544279763 1106 6 -0.11111220099414965 1106 7 -0.0744678431015231 1106 8 0.03028029647629893 1106 9 0.06942881493454678 1106 10-11 0.060935005570485146 1106 12-13 0.12769728074963638 1106 14-15 0.27533968899341943 1106 16-17 0.08136642705218122 1106 18-19 0.16597859941231263 1106 20-21 0.09920254385267668 1106 22-23 0.08882819573968703 1106 24-25 0.10570687518860211 1106 26-27 0.20900251594103736 1106 28-29 0.17337485947774667 1106 30-31 0.06577842950735402 1106 32-33 -0.009667861454076387 1106 34-35 0.2232405055310167 1106 36-37 0.09257209564273694 1106 38-39 0.09155747191920227 1106 40-41 0.32116420996342754 1106 42-43 -0.05282798095791463 1106 44-45 0.041673909650384644 1106 46-47 0.2258355943521977 1106 48-49 0.30184816336402776 1106 50-51 0.21796020661869875 1106 52-53 0.2330760542629804 1106 54-55 -0.4491609461976296 1106 56-57 -0.05042455112924671 1106 58-59 0.20891966284030872 1106 60-61 0.33631075492842655 1106 62-63 -0.1960567556366115 1106 64-65 0.15304383080778905 1106 66-67 0.278942612986647 1106 68-69 0.08356377694595096 1106 70-71 0.17907038888496274 1106 72-73 0.3728228871124202 1106 74-75 0.5755541329951122 1106 76-77 0.7315584467555709 1106 78-79 0.7617137364450137 1106 80-81 0.642069652819945 1106 82-83 0.8545357361450918 1106 84-85 1.1446712375587218 1106 86-87 0.9190691928550692 1106 88-89 1.3810831457349124 1106 90-91 1.5524255110353415 1106 92-93 1.8492983468734607 1106 94-95 2.101640984660083 1106 96-97 2.1008857903415397 1106 98-99 2.2895864607644043 1106 100-101 2.8102047954330907 1107 1 0.27116838616323236 1107 2 0.09600469076199403 1107 3 0.1813344190793842 1107 4 0.18490287529244398 1107 5 0.16712789420977714 1107 6 0.12846596722965842 1107 7 0.20620964528513497 1107 8 0.2588127420632276 1107 9 0.12656172244537345 1107 10-11 0.15939966317265686 1107 12-13 0.14809443417622248 1107 14-15 0.22027336898715788 1107 16-17 0.2895335254132618 1107 18-19 0.19398518248031849 1107 20-21 0.32150831398681134 1107 22-23 0.3533212212107415 1107 24-25 0.3474417484857497 1107 26-27 0.30323450221380455 1107 28-29 0.4628622576978998 1107 30-31 0.430244460484964 1107 32-33 0.43481604623943326 1107 34-35 0.5090131026133307 1107 36-37 0.5491154466788046 1107 38-39 0.5163990117960182 1107 40-41 0.5173020400954798 1107 42-43 0.6270625894006585 1107 44-45 0.46865543595656334 1107 46-47 0.32886648061975166 1107 48-49 0.2728168497516208 1107 50-51 0.06800579550642283 1107 52-53 0.10795698489610572 1107 54-55 0.31690036963304635 1107 56-57 0.1435806324659339 1107 58-59 0.10956320830197086 1107 60-61 0.218246953320822 1107 62-63 0.3607421867664371 1107 64-65 0.41143373568278463 1107 66-67 0.5257334488862284 1107 68-69 0.5185763689392715 1107 70-71 0.6423798974709989 1107 72-73 0.9173756266865105 1107 74-75 1.1699773642441365 1107 76-77 1.0326763123800795 1107 78-79 1.0270373930815815 1107 80-81 0.939052304576304 1107 82-83 1.2938140289839666 1107 84-85 1.6054668329708015 1107 86-87 1.6750581373459141 1107 88-89 1.9303060957844984 1107 90-91 1.6967139329258352 1107 92-93 2.051298304465192 1107 94-95 2.7496711206275144 1107 96-97 2.949627324998879 1107 98-99 2.9653984975356344 1107 100-101 3.071569803392798 1108 1 0.24187638100794118 1108 2 0.12888042271730882 1108 3 0.04343411365422156 1108 4 0.18281792326703084 1108 5 0.1971692867523771 1108 6 0.2289152931641354 1108 7 0.2593226679215377 1108 8 0.23232902399839617 1108 9 0.29395862038003884 1108 10-11 0.1594996581154433 1108 12-13 0.21290456638409694 1108 14-15 0.3169857191671426 1108 16-17 0.3463636014400677 1108 18-19 0.26103504815760914 1108 20-21 0.30940586093080213 1108 22-23 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-2.39777427597841 2316 86-87 -2.0125340354265653 2316 88-89 -2.453521411360903 2316 90-91 -2.538211430507001 2316 92-93 -2.5999021414593955 2316 94-95 -2.713529982655661 2316 96-97 -2.8296179267811077 2316 98-99 -2.8444074897010623 2316 100-101 -2.9898686958673686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5503.0 3 906.0 4 768.0 5 734.0 6 777.0 7 879.0 8 1031.0 9 1217.0 10 1599.0 11 2291.0 12 3446.0 13 4902.0 14 5789.0 15 7556.0 16 9405.0 17 12145.0 18 11847.0 19 9225.0 20 9074.0 21 9188.0 22 10185.0 23 11314.0 24 13019.0 25 14569.0 26 17226.0 27 19931.0 28 23682.0 29 27907.0 30 33309.0 31 40769.0 32 50715.0 33 64585.0 34 88598.0 35 130016.0 36 152221.0 37 181268.0 38 111048.0 39 7602.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.69625266792705 17.370645058710434 13.653829764031075 19.279272509331445 2 13.436718916112216 22.979231324481013 40.82246108668018 22.761588672726592 3 23.416119761506454 28.581441639264366 30.178578110468912 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29.226070640458644 26.643172779092968 25.256628817262396 24-25 20.65359763483201 29.198214258005216 29.76650446754968 20.381683639613097 26-27 23.914115888788746 25.983411862685816 30.868857882787808 19.233614365737626 28-29 19.560871685993003 27.006176853204256 33.91697911405026 19.515972346752484 30-31 18.79575291566109 32.83363609026948 30.89225001562837 17.47836097844106 32-33 18.40163940036495 31.405915402597177 28.899724530728033 21.29272066630984 34-35 20.117337407333384 27.762795362721988 29.256139063582324 22.863728166362304 36-37 22.582920763842942 27.89851931281513 31.05591932650407 18.462640596837854 38-39 24.86883812937168 24.489312702673605 32.45738493796386 18.18446422999086 40-41 19.768039696844863 27.04628120362425 35.22105024012175 17.96462885940914 42-43 17.96863880058832 30.984467209973687 31.83747504571151 19.209418943726483 44-45 18.64238387939194 32.417931553558596 28.421079040700658 20.5186055263488 46-47 18.442638656474287 29.104218864908365 26.306134589801655 26.147007888815693 48-49 20.835553544186453 28.113663238417796 28.65121278257505 22.399570434820692 50-51 24.484189725123187 29.14557276722462 28.611584257146532 17.75865325050566 52-53 19.45766045709208 28.819098205186922 32.914881174488166 18.808360163232834 54-55 17.210638364841557 28.407032454922703 33.517564031039434 20.8647651491963 56-57 20.115490180962595 32.29478232349749 27.548363052812654 20.04136444272726 58-59 18.418965083886167 29.357033149849215 29.10222028403469 23.121781482229927 60-61 19.337555664428685 26.24254500368131 30.794095670285937 23.625803661604067 62-63 25.473807231037103 25.42312729422509 28.82132881790758 20.281736656830226 64-65 23.12915395107176 26.4206113521182 31.79496302783974 18.6552716689703 66-67 18.711846872509767 27.542960256203823 33.17549423737534 20.569698633911063 68-69 18.042334703618756 31.775477547207338 31.02583471547875 19.156353033695154 70-71 18.845316748924205 30.09677026448167 27.878485415120398 23.179427571473735 72-73 18.580434733024656 28.637456378441257 26.76988344775677 26.01222544077732 74-75 20.578713597757876 28.21640474644958 29.111301989273823 22.093579666518718 76-77 23.141835644606708 25.261027469805658 30.33606745068417 21.26106943490347 78-79 19.921915804272235 25.76550382734808 32.80256941615665 21.510010952223034 80-81 19.193807445644314 30.855162050943786 30.117129926771558 19.833900576640342 82-83 19.199124177490905 29.75661713555862 28.24609700190446 22.798161685046015 84-85 20.309532899901516 26.444130310531865 28.61128938894872 24.6350474006179 86-87 22.44162492810446 26.444467453043835 30.02093123707762 21.092976381774086 88-89 24.730664262635536 23.683980009799832 30.92098359668485 20.664372130879784 90-91 20.656662458578772 25.638706987053173 33.188719282821374 20.51591127154668 92-93 19.294704237431173 28.841628917993 31.0760310747994 20.78763576977643 94-95 19.59630367059708 29.966246858740504 28.388362785587713 22.049086685074705 96-97 19.687351014980052 27.18668379020172 26.46936582851069 26.656599366307542 98-99 21.654287430935636 26.328560929243327 27.908230788917948 24.10892085090309 100-101 24.800343130268914 25.908573506250526 28.62625476417559 20.66482859930497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6663.0 1 3926.5 2 1695.0 3 2099.0 4 1465.0 5 1440.0 6 3429.0 7 6717.5 8 8853.0 9 8418.0 10 5999.5 11 4001.0 12 3544.5 13 3296.0 14 2840.0 15 2265.0 16 1916.0 17 1877.5 18 1937.5 19 2081.0 20 2309.5 21 2571.0 22 2966.5 23 3548.0 24 4299.5 25 5032.5 26 6110.5 27 7504.0 28 8844.0 29 10343.0 30 11761.5 31 13355.0 32 15239.0 33 17002.0 34 18687.0 35 20215.5 36 21565.0 37 22630.0 38 23233.0 39 23856.0 40 24653.5 41 25250.0 42 25231.0 43 25210.0 44 26488.0 45 28472.5 46 35926.5 47 54860.5 48 147358.5 49 156084.5 50 64390.5 51 36967.5 52 26386.0 53 20688.5 54 16995.0 55 14445.0 56 12172.0 57 9858.5 58 7820.0 59 6036.0 60 4506.5 61 3410.0 62 2609.0 63 1918.0 64 1401.5 65 1060.0 66 874.0 67 710.5 68 511.5 69 362.0 70 286.5 71 243.5 72 217.0 73 176.0 74 130.0 75 97.0 76 70.0 77 41.5 78 26.5 79 21.5 80 19.0 81 14.0 82 14.0 83 12.5 84 6.0 85 2.5 86 2.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4600244835556983 2 0.03767402572050434 3 0.048802914674261066 4 0.053728816342317326 5 0.077263679867475 6 0.07452706782966596 7 0.04159650297469729 8 0.03493741368269531 9 0.040501858159573675 10-11 0.04602069243582189 12-13 0.041186011169025934 14-15 0.03525668508710636 16-17 0.03680743190853148 18-19 0.044606776216287225 20-21 0.05222367972152236 22-23 0.04561020063015053 24-25 0.061938652455744425 26-27 0.05322710413538567 28-29 0.042098215181628935 30-31 0.04401384360809526 32-33 0.043740182404314355 34-35 0.04716094745157565 36-37 0.042964808993601805 38-39 0.024811949142801888 40-41 0.021847286101842105 42-43 0.022075337104992855 44-45 0.012177923568250192 46-47 0.02408218593271948 48-49 0.02316998192011647 50-51 0.014139162195346663 52-53 0.013044517380223053 54-55 0.014275992797237114 56-57 0.012360364370770795 58-59 0.013455009185894408 60-61 0.01541624781299088 62-63 0.013819890790935612 64-65 0.01377428059030546 66-67 0.007981785110276344 68-69 0.01131132975627733 70-71 0.01792480884764916 72-73 0.015598688615511483 74-75 0.012998907179592902 76-77 0.008848378922249203 78-79 0.01108327875312658 80-81 0.014184772395976816 82-83 0.012177923568250192 84-85 0.010079854339263268 86-87 0.00342076504726129 88-89 0.02818710398943303 90-91 0.031060546629132515 92-93 0.016510892628114494 94-95 0.03174469963858477 96-97 0.026317085763596856 98-99 0.023489253324527525 100-101 0.14759460923916712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1096246.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.467379026058694 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68826376835953 28.68815256891976 2 14.896586748755581 11.758584708153998 3 5.768232498692391 6.829710550089667 4 2.657063628591974 4.194693493039772 5 1.383270387433825 2.729702833818692 6 0.8252298438945722 1.9541795419561396 7 0.4934836273454393 1.3633553754517733 8 0.3206586814459857 1.0124446174899948 9 0.20996579611231844 0.745811969190573 >10 0.6498788255113033 4.097279748224814 >50 0.045915118126013864 1.2898248204547944 >100 0.044986825259742784 3.6130755680235915 >500 0.005565380441018939 1.5760872976464624 >1k 0.006492943847855429 5.628556344303898 >5k 0.002318908517091225 7.013272726244027 >10k+ 0.0020870176653821023 17.505267836991976 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 45904 4.18738129945286 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35616 3.2489058112868827 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 27976 2.5519819456581825 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16605 1.514714762927299 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 14852 1.3548053995179914 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14197 1.2950560366924941 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12865 1.1735504622137733 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12168 1.1099698425353433 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 11087 1.0113605887729578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9713 0.8860237574413043 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8945 0.8159664892733931 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8887 0.8106757060002955 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7641 0.6970150860299604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7446 0.6792271077842017 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7357 0.6711084920720349 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7071 0.6450194573115888 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6706 0.6117240108515789 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6447 0.5880979269251609 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6417 0.5853613148873519 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4464 0.40720787122598395 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4232 0.3860447381335941 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3958 0.36105034818827164 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3389 0.30914593987116035 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2979 0.27174557535443683 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2916 0.2659986900750379 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.24574776099525109 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.2418252837410581 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2512 0.22914564796587628 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2495 0.22759490114445113 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2230 0.20342149481047136 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.19466433628948246 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2025 0.18472131255210963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1971 0.1797954108840534 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1885 0.1719504563756675 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1846 0.16839286072651574 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.1618249918357741 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1740 0.15872349819292386 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1682 0.15343271491982638 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1527 0.1392935527244797 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1495 0.1363744998841501 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1473 0.13436765105642345 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1439 0.13126615741357323 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1389 0.1267051373505582 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.11758309722452807 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1172 0.10691031027707285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.122040126030107E-5 0.0 12-13 0.0 0.0 0.0 9.122040126030107E-5 0.0 14-15 0.0 0.0 0.0 2.736612037809032E-4 0.0 16-17 0.0 0.0 0.0 5.473224075618064E-4 0.0 18-19 0.0 0.0 0.0 0.0012770856176442147 0.0 20-21 0.0 0.0 0.0 0.0025541712352884294 0.0 22-23 0.0 0.0 0.0 0.0062942076869607734 0.0 24-25 0.0 0.0 0.0 0.012588415373921547 0.0 26-27 0.0 0.0 0.0 0.022850710515705416 0.0 28-29 0.0 0.0 0.0 0.049259016680562574 0.0 30-31 0.0 0.0 0.0 0.1031702738254005 0.0 32-33 0.0 0.0 0.0 0.19087868963717997 0.0 34-35 0.0 0.0 0.0 0.3868201115443066 0.0 36-37 0.0 0.0 0.0 0.6597971623157576 0.0 38-39 0.0 0.0 0.0 0.9100603331733936 0.0 40-41 0.0 0.0 0.0 1.1626952344637973 0.0 42-43 0.0 0.0 0.0 1.4105866748886655 0.0 44-45 0.0 0.0 0.0 1.6808271136223074 0.0 46-47 0.0 0.0 0.0 2.036997170343153 0.0 48-49 0.0 0.0 0.0 2.457888101758182 0.0 50-51 0.0 0.0 0.0 2.795403586421296 0.0 52-53 0.0 0.0 0.0 3.0716645716381175 0.0 54-55 0.0 0.0 0.0 3.360513972228861 0.0 56-57 0.0 0.0 0.0 3.6512333910454404 0.0 58-59 0.0 0.0 0.0 4.095932847189408 0.0 60-61 0.0 0.0 0.0 4.731921484776228 0.0 62-63 0.0 0.0 0.0 5.19053205211239 0.0 64-65 0.0 0.0 0.0 5.525584585941477 0.0 66-67 0.0 0.0 0.0 5.917604260357621 0.0 68-69 0.0 0.0 0.0 6.32640848860566 0.0 70-71 0.0 0.0 0.0 6.782738545910316 0.0 72-73 0.0 0.0 0.0 7.332295853303 0.0 74-75 0.0 0.0 0.0 7.853164344499318 0.0 76-77 0.0 0.0 0.0 8.295902562016188 0.0 78-79 0.0 0.0 0.0 8.767968138538247 0.0 80-81 0.0 0.0 0.0 9.246282312546636 0.0 82-83 0.0 0.0 0.0 9.727424318994094 0.0 84-85 0.0 0.0 0.0 10.527928950253866 0.0 86-87 0.0 0.0 0.0 11.378696022608064 0.0 88-89 0.0 0.0 0.0 11.929667246220284 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGC 405 0.0 29.466383 1 GTAGCCC 625 0.0 27.367664 3 GGACGCA 35 0.008401356 27.137455 72-73 TAGCCCT 900 0.0 26.924206 4 GTGTAGC 680 0.0 23.867773 1 CGCCGGA 80 1.8607388E-6 23.750149 92-93 GTCCTAC 165 2.3274715E-6 23.144506 1 GAGTACT 6595 0.0 23.084446 12-13 CATGGGG 3005 0.0 22.768438 4 TGTAGCC 755 0.0 22.65535 2 CCGGATC 85 3.1211512E-6 22.377588 94-95 TACCACG 110 5.57975E-8 21.614716 94-95 GTACTTT 7185 0.0 21.05759 14-15 AGTACTT 6585 0.0 20.81116 12-13 CGTATCA 290 0.0 20.496712 94-95 GTATTAC 140 4.692345E-4 20.45809 1 ATGGGGA 2085 0.0 20.053577 5 AGTACTC 570 0.0 20.005602 5 AGCCCTG 1245 0.0 19.844915 5 CCGTATC 360 0.0 19.81349 94-95 >>END_MODULE