##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1096246 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45920897316843 33.0 31.0 34.0 31.0 34.0 2 32.66362294594462 34.0 31.0 34.0 31.0 34.0 3 32.63021256177902 34.0 31.0 34.0 31.0 34.0 4 36.080418081343055 37.0 35.0 37.0 35.0 37.0 5 36.07816949845199 37.0 35.0 37.0 35.0 37.0 6 36.18103144732113 37.0 36.0 37.0 35.0 37.0 7 36.166552033029085 37.0 36.0 37.0 35.0 37.0 8 36.20117199971539 37.0 37.0 37.0 35.0 37.0 9 37.85930986293223 39.0 38.0 39.0 35.0 39.0 10-11 37.84331254116321 39.0 38.0 39.0 35.0 39.0 12-13 37.796480899360176 39.0 38.0 39.0 35.0 39.0 14-15 38.85614086619245 40.0 38.0 41.0 35.0 41.0 16-17 38.82588077858436 40.0 38.0 41.0 35.0 41.0 18-19 38.96608471091343 40.0 38.5 41.0 35.5 41.0 20-21 39.02983363223218 40.0 38.5 41.0 35.0 41.0 22-23 39.098942664328995 40.0 39.0 41.0 35.0 41.0 24-25 39.028071710181834 40.0 38.5 41.0 35.0 41.0 26-27 38.6324839497704 40.0 38.0 41.0 34.5 41.0 28-29 38.47122498052444 40.0 38.0 41.0 34.5 41.0 30-31 38.37512474389872 40.0 38.0 41.0 34.5 41.0 32-33 38.22541838237038 40.0 37.0 41.0 34.0 41.0 34-35 38.066778350844615 40.0 37.0 41.0 34.0 41.0 36-37 37.889039047804964 40.0 37.0 41.0 33.5 41.0 38-39 37.70165090682201 40.0 36.5 41.0 33.0 41.0 40-41 37.50284835702935 40.0 36.5 41.0 33.0 41.0 42-43 37.48943850194208 40.0 37.0 41.0 33.0 41.0 44-45 37.332560392466654 40.0 36.5 41.0 33.0 41.0 46-47 36.96924504171509 40.0 36.0 41.0 32.0 41.0 48-49 36.65057979687042 40.0 36.0 41.0 31.0 41.0 50-51 36.39296380556919 40.0 35.5 41.0 30.5 41.0 52-53 35.668755461821526 39.0 35.0 40.5 28.0 41.0 54-55 35.51023538512341 39.0 35.0 40.0 28.0 41.0 56-57 35.46622975135143 39.0 35.0 40.0 28.0 41.0 58-59 35.242772607608146 38.5 35.0 40.0 27.5 41.0 60-61 34.832324587729396 38.0 34.5 40.0 26.5 41.0 62-63 34.31931610240767 38.0 34.0 40.0 25.5 41.0 64-65 33.64615880012333 37.0 33.0 39.5 22.0 41.0 66-67 33.15993901003972 36.0 33.0 39.0 22.0 40.5 68-69 32.74979657850518 36.0 32.5 39.0 21.5 40.0 70-71 32.14333735311235 35.5 31.5 37.5 19.0 39.5 72-73 31.60301474304125 35.0 31.0 37.0 17.5 39.0 74-75 31.074804833951504 35.0 31.0 37.0 13.0 39.0 76-77 29.58709404640929 33.5 29.0 35.0 9.5 37.0 78-79 30.209523227450774 34.0 30.0 36.0 7.5 37.5 80-81 30.19902239095969 34.0 31.0 35.5 6.5 37.0 82-83 29.8477271524822 34.0 30.0 35.0 2.0 37.0 84-85 29.446049518082617 34.0 30.0 35.0 2.0 36.0 86-87 28.895403951302903 34.0 29.0 35.0 2.0 36.0 88-89 28.153853240969635 33.5 28.0 35.0 2.0 35.0 90-91 27.60406970698183 33.0 26.5 35.0 2.0 35.0 92-93 27.254254063412773 33.0 26.0 35.0 2.0 35.0 94-95 26.697814176745002 33.0 25.0 35.0 2.0 35.0 96-97 25.895580006677335 32.0 24.0 34.5 2.0 35.0 98-99 24.99664673804967 32.0 19.0 34.0 2.0 35.0 100-101 21.666368223920543 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.030473691902997757 1101 2 0.040938737418123594 1101 3 0.14462054627679066 1101 4 0.01742626641288325 1101 5 -0.017045455434946177 1101 6 0.08026898738139465 1101 7 1.9169428049536918E-4 1101 8 0.08719255676120241 1101 9 0.06575975818569901 1101 10-11 0.09662851810976036 1101 12-13 2.1139687704874177E-4 1101 14-15 0.11051466136645871 1101 16-17 0.23749486147524834 1101 18-19 0.2939260634385974 1101 20-21 0.1923853801482096 1101 22-23 0.06415063056432047 1101 24-25 0.11669570513319627 1101 26-27 0.18142983837382332 1101 28-29 0.13052755820484663 1101 30-31 0.14680605058585883 1101 32-33 0.10512665406412935 1101 34-35 0.039813093119448695 1101 36-37 0.0836047535732618 1101 38-39 0.2856423475645897 1101 40-41 0.2561657843350318 1101 42-43 0.06873182980083925 1101 44-45 0.3061991632122485 1101 46-47 0.15678547892892425 1101 48-49 0.06542649247601418 1101 50-51 -0.09771777258492165 1101 52-53 0.16076209500621275 1101 54-55 0.212491354255107 1101 56-57 0.2876305621735824 1101 58-59 0.1613682727854595 1101 60-61 0.08509818960219206 1101 62-63 0.13845655141692248 1101 64-65 0.23967257022380295 1101 66-67 0.20629169129930602 1101 68-69 0.2909001931979276 1101 70-71 0.3419715158358585 1101 72-73 0.25339292728442686 1101 74-75 0.19475811760895922 1101 76-77 0.20909378222163966 1101 78-79 0.235241862826431 1101 80-81 0.5419088059010662 1101 82-83 0.44655160691888796 1101 84-85 0.5596529782059214 1101 86-87 0.7588746788976586 1101 88-89 0.6931145015836897 1101 90-91 0.6094949035859472 1101 92-93 0.8102598037438504 1101 94-95 1.0635076883246448 1101 96-97 1.4343019923211742 1101 98-99 1.6374701248093224 1101 100-101 2.3365796434562576 1102 1 -0.004010256891952224 1102 2 0.09318610574146646 1102 3 0.10771704418196748 1102 4 0.06709672665461142 1102 5 0.06214929173225414 1102 6 0.043163738880572566 1102 7 0.13674678156985465 1102 8 0.08945508700769267 1102 9 0.026130823047786578 1102 10-11 0.0789206295777447 1102 12-13 0.1274044607613476 1102 14-15 0.0718078186977138 1102 16-17 0.0029411480417849134 1102 18-19 0.06436402641875105 1102 20-21 0.09986732508258456 1102 22-23 0.2098171053862501 1102 24-25 0.06874937215260957 1102 26-27 0.04475159913723559 1102 28-29 -0.09058712163103166 1102 30-31 -0.009945956048255766 1102 32-33 2.84740320005028E-4 1102 34-35 -0.06488834076527894 1102 36-37 0.12747098209128893 1102 38-39 0.22485974478840376 1102 40-41 0.007676097537036242 1102 42-43 -0.0773486583511982 1102 44-45 0.0015410491079492772 1102 46-47 -0.0596766564887119 1102 48-49 0.09895167055125853 1102 50-51 0.242780033577624 1102 52-53 0.2610442924330201 1102 54-55 0.27963887048455405 1102 56-57 0.32841366003296457 1102 58-59 0.2208481878786941 1102 60-61 0.08822649660328352 1102 62-63 -0.03212251027663626 1102 64-65 -0.027258965957500436 1102 66-67 -0.10934489287412674 1102 68-69 0.13149887256347625 1102 70-71 0.1592554893736633 1102 72-73 0.1669161250268445 1102 74-75 0.010067946867138744 1102 76-77 0.011122385653802525 1102 78-79 0.23578831091386476 1102 80-81 0.31699560017542794 1102 82-83 0.41903240697335065 1102 84-85 0.558060666848089 1102 86-87 0.6180197939735628 1102 88-89 0.5515374140344136 1102 90-91 0.624991007899979 1102 92-93 0.8700510024021746 1102 94-95 1.2562268709422746 1102 96-97 1.3132801405862153 1102 98-99 1.4211261905068646 1102 100-101 1.383434936823999 1103 1 0.2691935480058447 1103 2 0.2926801352901407 1103 3 0.404843132018442 1103 4 0.19532987783858147 1103 5 0.017259593290638975 1103 6 0.15733091151768974 1103 7 0.20859458565809064 1103 8 0.21145468223132724 1103 9 0.1682680422341889 1103 10-11 0.3450003705208715 1103 12-13 0.3344374500347769 1103 14-15 0.4882316195477401 1103 16-17 0.3376304821846716 1103 18-19 0.2973634192542036 1103 20-21 0.3301648357079614 1103 22-23 0.3328893579666996 1103 24-25 0.33569938227201845 1103 26-27 0.41090217594355494 1103 28-29 0.35397875572172666 1103 30-31 0.4974715707681767 1103 32-33 0.593221392819494 1103 34-35 0.5601268383482605 1103 36-37 0.4685760215569914 1103 38-39 0.8349083179517294 1103 40-41 0.7893397283810017 1103 42-43 0.6601842297281308 1103 44-45 0.8409460278571927 1103 46-47 0.8959092572939298 1103 48-49 0.9581805715834335 1103 50-51 1.0664392118816224 1103 52-53 1.0475348813826244 1103 54-55 1.1954150291568837 1103 56-57 1.1126071431335518 1103 58-59 1.2828396875751125 1103 60-61 1.1970405018653736 1103 62-63 0.9999661357464404 1103 64-65 1.1557100686508832 1103 66-67 1.1309809520965288 1103 68-69 1.4324399776029395 1103 70-71 1.3238780354169748 1103 72-73 1.0732508750774379 1103 74-75 0.9533183010464512 1103 76-77 0.7275158282767542 1103 78-79 1.1704270480116143 1103 80-81 1.2619156568441205 1103 82-83 1.3712485575501532 1103 84-85 1.591707701861246 1103 86-87 1.5119380291486273 1103 88-89 1.7801955803974963 1103 90-91 1.8328335498955255 1103 92-93 2.265284760750692 1103 94-95 2.0801289150628968 1103 96-97 2.1402686044599264 1103 98-99 2.622219086681728 1103 100-101 2.9668188672024627 1104 1 0.05746515294411125 1104 2 0.08213692541359308 1104 3 0.17141103325300833 1104 4 0.08244098894969198 1104 5 -0.036320653622937016 1104 6 0.07554078806089848 1104 7 0.011063721460566 1104 8 -0.0011132190032370204 1104 9 0.012119894086040972 1104 10-11 0.14104358039741527 1104 12-13 0.07438806731872205 1104 14-15 0.19349087880700466 1104 16-17 0.13809961798714454 1104 18-19 0.1705115674023503 1104 20-21 0.23807224311536856 1104 22-23 0.1369263950037407 1104 24-25 0.16223024646954798 1104 26-27 0.13666143520281793 1104 28-29 0.15490468164765048 1104 30-31 -0.0010716391083676058 1104 32-33 0.14517818294294926 1104 34-35 0.07675646797788716 1104 36-37 0.0747797252606901 1104 38-39 0.1683897994332142 1104 40-41 0.13241380245506917 1104 42-43 0.14249287170343905 1104 44-45 0.3076312130423773 1104 46-47 0.1404462943309639 1104 48-49 0.31866205306491935 1104 50-51 0.17026637237544406 1104 52-53 0.35983117767892026 1104 54-55 0.5648656464408361 1104 56-57 0.5603972665903427 1104 58-59 0.6072470949825188 1104 60-61 0.46192321791475877 1104 62-63 0.5383365061168064 1104 64-65 0.5735989575397653 1104 66-67 0.6153081125903554 1104 68-69 0.6516436813066449 1104 70-71 0.8868101341824044 1104 72-73 0.8700718627317663 1104 74-75 0.749398547960034 1104 76-77 0.7578491616100855 1104 78-79 0.9007418628264325 1104 80-81 0.8729218296836265 1104 82-83 1.052674483476082 1104 84-85 1.0424978253180335 1104 86-87 1.0925553130992434 1104 88-89 1.23026965447157 1104 90-91 1.1160866363152806 1104 92-93 1.3019143903803183 1104 94-95 1.391196816297466 1104 96-97 1.5610834192747411 1104 98-99 2.048385753348395 1104 100-101 2.3164731881901197 1105 1 0.08547267174110829 1105 2 0.0567384291729951 1105 3 0.2614035144560134 1105 4 0.1056891092504415 1105 5 0.02116054938458234 1105 6 -0.0020907908864700175 1105 7 0.06858251845304153 1105 8 -0.020105700206244137 1105 9 -0.037774842756064686 1105 10-11 0.05152478340492905 1105 12-13 0.0372790447623359 1105 14-15 -0.018580549764429577 1105 16-17 0.09498683603225544 1105 18-19 0.13413938695122596 1105 20-21 0.07760983710032576 1105 22-23 -0.014942026048892387 1105 24-25 0.07373776526654297 1105 26-27 0.09835692392461937 1105 28-29 0.1739836290160781 1105 30-31 0.040853172921707426 1105 32-33 -0.012175200515699203 1105 34-35 0.05188052812826527 1105 36-37 0.1596857402982863 1105 38-39 0.01826949868133454 1105 40-41 0.04800026862049123 1105 42-43 0.10955302207938189 1105 44-45 0.20883797995967512 1105 46-47 0.12815306124826265 1105 48-49 -0.013439450694484378 1105 50-51 0.29052953027018447 1105 52-53 0.3008800498593658 1105 54-55 0.42043707501226635 1105 56-57 0.39937846959785617 1105 58-59 0.5375215310727413 1105 60-61 0.5386224660350649 1105 62-63 0.4591522955904921 1105 64-65 0.5704448222014236 1105 66-67 0.602815631387351 1105 68-69 0.6118955610058947 1105 70-71 0.5435394574906738 1105 72-73 0.22512825370920453 1105 74-75 0.28231208179462186 1105 76-77 0.26357472551986305 1105 78-79 0.46503509590913694 1105 80-81 0.6818729575031739 1105 82-83 0.7146556864835993 1105 84-85 0.7301707576488639 1105 86-87 0.8133071927984936 1105 88-89 1.0296455943211953 1105 90-91 1.0821918994731767 1105 92-93 1.1298767963953544 1105 94-95 1.284715613289947 1105 96-97 1.506023268898801 1105 98-99 1.7211225954536573 1105 100-101 2.045356646836737 1106 1 0.02768574117941114 1106 2 0.06062221953123981 1106 3 0.037940445017710545 1106 4 0.06452922424380603 1106 5 0.03511316990647373 1106 6 -0.051481270762629094 1106 7 0.056468133225266115 1106 8 0.015857369232058716 1106 9 -0.03337275297278808 1106 10-11 0.002517845103298555 1106 12-13 -0.06322957974010279 1106 14-15 -0.03256426825182501 1106 16-17 -0.055672440836389114 1106 18-19 0.04008877328470817 1106 20-21 -0.05829172747286293 1106 22-23 -0.020698604996262304 1106 24-25 0.05777068764601978 1106 26-27 0.04120922932046511 1106 28-29 -0.11798134776411473 1106 30-31 0.0888511550092872 1106 32-33 0.07099803588412357 1106 34-35 -0.09461853202211046 1106 36-37 -0.03709527473930763 1106 38-39 0.21537877002144512 1106 40-41 0.28234762598448526 1106 42-43 0.18218404817402956 1106 44-45 0.30168268363060946 1106 46-47 -0.03727429390432491 1106 48-49 -0.004455593993910156 1106 50-51 0.030854607669560608 1106 52-53 0.10403338356126568 1106 54-55 0.19453108761730675 1106 56-57 0.35266932541387774 1106 58-59 0.36028385968840126 1106 60-61 0.24623939438534848 1106 62-63 0.14147621199916216 1106 64-65 0.16408425165742102 1106 66-67 0.14107649494329877 1106 68-69 0.05333118130664616 1106 70-71 0.27919984006475573 1106 72-73 0.23933656861411734 1106 74-75 0.24419634207768226 1106 76-77 0.48584916161008707 1106 78-79 0.413550686355844 1106 80-81 0.41640344733068346 1106 82-83 0.40186198347608126 1106 84-85 0.35419635472980104 1106 86-87 0.74141560721689 1106 88-89 0.6724681838833355 1106 90-91 0.5355829598446924 1106 92-93 0.2460173315567893 1106 94-95 0.5262776986504072 1106 96-97 0.7932268016276822 1106 98-99 0.9385181062895676 1106 100-101 1.2884805411312925 1107 1 0.1596390659875908 1107 2 0.1129426042068502 1107 3 0.2178493651074902 1107 4 0.1273815390827835 1107 5 0.13792956820492464 1107 6 0.09601549968468248 1107 7 0.06039823787582321 1107 8 -0.019031586350173768 1107 9 0.0517969127195812 1107 10-11 0.08427649965207706 1107 12-13 0.051706612127951246 1107 14-15 0.131208713767073 1107 16-17 0.12146607761446404 1107 18-19 0.16365708647955302 1107 20-21 0.23837082341705695 1107 22-23 0.07445168337995511 1107 24-25 0.15239617371000946 1107 26-27 0.05441165703067696 1107 28-29 0.08860033382156729 1107 30-31 0.07758319673902037 1107 32-33 0.2060304455871389 1107 34-35 0.2699694227250049 1107 36-37 0.29987511124117106 1107 38-39 0.2446506689984318 1107 40-41 0.3285424626147915 1107 42-43 0.3293260571515333 1107 44-45 0.4322536620219708 1107 46-47 0.4785097370993796 1107 48-49 0.46401875226634104 1107 50-51 0.3930081647445647 1107 52-53 0.4233786488413003 1107 54-55 0.42936564644083575 1107 56-57 0.4352357759071097 1107 58-59 0.1991202094456952 1107 60-61 0.3080469978615241 1107 62-63 0.32841104027829715 1107 64-65 0.2080860915237963 1107 66-67 0.16478149324697 1107 68-69 0.35073995459856633 1107 70-71 0.4348292113785064 1107 72-73 0.3981131227140118 1107 74-75 0.4157006775075054 1107 76-77 0.32535670375738235 1107 78-79 0.6367502922851713 1107 80-81 0.7256897977404115 1107 82-83 0.799481493236506 1107 84-85 0.8605208954156431 1107 86-87 0.8998667594169021 1107 88-89 0.8532048807359907 1107 90-91 0.8150089965637299 1107 92-93 1.0211344436190046 1107 94-95 1.0234811996160111 1107 96-97 1.2766007218479416 1107 98-99 1.5131093558328637 1107 100-101 1.8618378731945526 1108 1 -0.08234486432704102 1108 2 -0.03792179652077721 1108 3 0.10020550648271609 1108 4 -0.027928613813692493 1108 5 -0.1394985465417662 1108 6 -0.04833658672320951 1108 7 -0.06112971549971036 1108 8 -0.109987139555912 1108 9 -0.05345523544764319 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 7.0 5 28.0 6 60.0 7 135.0 8 290.0 9 538.0 10 919.0 11 1601.0 12 2501.0 13 3773.0 14 4893.0 15 6288.0 16 7851.0 17 10682.0 18 10860.0 19 8343.0 20 7180.0 21 6990.0 22 7667.0 23 8477.0 24 9593.0 25 11021.0 26 11912.0 27 13741.0 28 16409.0 29 19878.0 30 24508.0 31 30012.0 32 38257.0 33 50345.0 34 71623.0 35 119556.0 36 164586.0 37 236613.0 38 175765.0 39 13340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.808296677935424 17.69155828162657 13.888762193887139 19.611382846550864 2 13.493741122118594 22.825540564334474 40.53559682626858 23.145121487278345 3 23.546787424100888 28.301337721858943 30.486538854507238 17.665335999532928 4 11.817694203673264 16.561884832419 39.134281903879234 32.4861390600285 5 12.264080376413238 41.0423063520646 33.78140957927173 12.912203692250435 6 26.100120046267072 37.45644217893096 18.7970013974991 17.646436377302862 7 24.44515190933422 37.99794936537967 19.44910175270879 18.107796972577322 8 21.41234722863299 32.611658332162676 21.377409814950294 24.598584624254045 9 24.696281674003828 21.568972657596927 21.68619087321641 32.04855479518283 10-11 22.54297849205379 25.576376105363213 27.55681662692498 24.32382877565802 12-13 31.13890495381511 23.9180804308522 25.253866376707418 19.689148238625272 14-15 26.254964670338595 25.6510856139954 29.209958348764786 18.883991366901228 16-17 18.947261357059162 29.386483228437427 31.44697059189753 20.219284822605886 18-19 17.657168190351435 32.75501119274323 31.625064082331882 17.962756534573444 20-21 20.110723323049754 30.101090448676665 27.98194930699861 21.80623692127497 22-23 18.76107187620297 29.218989168489557 26.553027331456626 25.46691162385085 24-25 20.649170042249327 29.188478153089154 29.630127970349303 20.53222383431222 26-27 23.82066175423998 26.113735816554072 30.635057062252052 19.430545366953893 28-29 19.563956166652833 26.86562194695884 33.909634998809565 19.66078688757876 30-31 18.768432239143625 32.51499436713509 31.133140858111098 17.583432535610196 32-33 18.129546936932417 31.454016356429683 28.95890823340525 21.457528473232653 34-35 19.88756059219548 27.68564956019623 29.331777453179896 23.09501239442839 36-37 22.300577201498697 27.908016322568695 31.124543972500142 18.666862503432466 38-39 24.697340538370472 24.394966206429334 32.47444667732671 18.43324657787348 40-41 19.818927525388467 26.86131919669334 35.14749491318193 18.17225836473626 42-43 18.10326186624624 30.802287165759303 32.05156980335374 19.042881164640715 44-45 18.67534866748013 32.27455497143378 28.602479159776944 20.447617201309143 46-47 18.374106704663838 29.000786337074057 26.30306634969924 26.32204060856286 48-49 20.61285682097856 27.91739605178779 28.791381108020758 22.67836601921289 50-51 24.22157070584522 28.96297911235252 28.833081260957847 17.982368920844408 52-53 19.36093723489071 28.573696049974185 33.06388347141061 19.001483243724493 54-55 17.05771332346937 28.139806211379565 33.63902810135681 21.163452363794256 56-57 20.117519242943647 32.228532646869404 27.393076006662735 20.260872103524207 58-59 18.19158291113491 29.446857730837788 29.176526071702884 23.18503328632442 60-61 19.132977452141215 26.57834099280636 30.430760978831394 23.85792057622103 62-63 24.980022732123995 26.068783831366314 28.454744646730752 20.49644878977894 64-65 22.808429859721265 26.824727296610433 31.44157424519679 18.92526859847151 66-67 18.440930322505903 28.068900750608783 32.65205221285235 20.838116714032967 68-69 17.86815611677515 32.096108209535565 30.430556714817463 19.605178958871818 70-71 18.553545463335787 30.506838793482483 27.246302381034916 23.693313362146817 72-73 18.159017227884984 28.88005976760691 26.198544852159095 26.762378152349015 74-75 20.058818914732644 28.459214446392505 28.452646577501767 23.029320061373085 76-77 22.450116123570805 25.583856178266558 29.868113543857856 22.09791415430478 78-79 19.264334829956052 25.86919359340878 32.37494139089219 22.49153018574298 80-81 18.60498210252462 30.78830990181018 29.753138899734 20.8535690959312 82-83 18.45457406486748 29.935004811924447 27.889822893813825 23.720598229394245 84-85 19.34216593907974 26.624299229112115 28.17223261921714 25.861302212591003 86-87 21.193829981030554 26.71307696833667 29.66476668127726 22.428326369355517 88-89 23.452649727022703 23.889139745221186 30.558178144484128 22.100032383271987 90-91 19.32748923445467 25.830877513385946 32.86901954058796 21.972613711571416 92-93 18.018204980029438 28.737314006030623 30.73163367278197 22.51284734115797 94-95 18.243300872479516 29.947634809237183 28.115833754088392 23.69323056419491 96-97 17.84355096776077 27.59358791215808 26.330454406964886 28.232406713116266 98-99 19.724541126127544 26.573840574653353 27.891613496454028 25.81000480276508 100-101 22.321564184349125 26.86388189891841 28.329472315383818 22.485081601348643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5874.0 1 3511.5 2 1638.0 3 2108.0 4 1476.5 5 1413.0 6 3507.0 7 6837.0 8 8774.0 9 8280.5 10 6051.5 11 3981.0 12 3495.5 13 3286.5 14 2800.0 15 2255.0 16 1843.0 17 1712.0 18 1784.5 19 1951.0 20 2155.5 21 2417.5 22 2816.0 23 3298.5 24 3921.5 25 4746.0 26 5782.0 27 7005.0 28 8372.5 29 9795.0 30 11407.5 31 13125.5 32 14820.0 33 16667.5 34 18494.5 35 20202.5 36 21704.5 37 22735.0 38 23368.5 39 23930.0 40 24891.5 41 25807.0 42 25921.5 43 25832.0 44 26850.0 45 28249.0 46 34388.5 47 50328.0 48 151330.0 49 154848.5 50 57601.5 51 45392.5 52 34447.5 53 22180.0 54 17153.5 55 14466.5 56 12089.5 57 9741.0 58 7706.0 59 5795.0 60 4027.0 61 2960.0 62 2181.5 63 1535.5 64 1085.0 65 763.5 66 617.5 67 509.0 68 356.5 69 220.5 70 162.0 71 131.5 72 98.5 73 67.0 74 42.0 75 24.0 76 14.0 77 8.0 78 5.0 79 3.5 80 4.5 81 2.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011220109355017032 3 0.004925901668056258 4 0.0 5 3.6488160504120426E-4 6 1.8244080252060213E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.561020063015053E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014139162195346664 26-27 0.022805100315075266 28-29 2.736612037809032E-4 30-31 9.122040126030107E-5 32-33 0.003740036451672343 34-35 0.0172406558381969 36-37 0.008665938119728602 38-39 0.02312437171948632 40-41 0.025176830747843094 42-43 0.01979482707348533 44-45 0.009897413536742665 46-47 0.0021892896302472256 48-49 2.736612037809032E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2805100315075266E-4 68-69 2.2805100315075266E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8244080252060213E-4 82-83 0.0010034244138633116 84-85 0.003876867053562795 86-87 8.665938119728602E-4 88-89 9.122040126030107E-5 90-91 0.0036944262510421933 92-93 6.84153009452258E-4 94-95 2.2805100315075266E-4 96-97 3.6488160504120426E-4 98-99 2.2805100315075266E-4 100-101 0.0029646630409597844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1096246.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.12399927865552 #Duplication Level Percentage of deduplicated Percentage of total 1 71.53705930690305 27.27278797413508 2 15.382350750090287 11.728734578009366 3 5.942058147520682 6.796050615894229 4 2.8153537265405273 4.293301737591648 5 1.5447854260809892 2.944669923479459 6 0.8750757963021834 2.0016833416196 7 0.5144318958514251 1.3728540858449985 8 0.3391058978571891 1.0342458404236246 9 0.21990860561488917 0.7545415969648959 >10 0.7164023886970171 4.297172103928189 >50 0.04749864929637093 1.3009641847006257 >100 0.04749797466282268 3.6348865052494546 >500 0.006476996541950073 1.7477086702349134 >1k 0.007436551585201937 6.244468879904221 >5k 0.002398887608129657 7.063998832493182 >10k+ 0.0021589988473166913 17.511931129526502 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 44223 4.034039804934294 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35183 3.2094073775411722 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28518 2.6014234031412657 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 17632 1.6083981150216284 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15740 1.4358091158371387 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14687 1.3397540333100417 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12919 1.1784763638818294 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12038 1.0981111903715042 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10541 0.9615542496848335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9674 0.8824661617921525 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9116 0.8315651778889046 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8915 0.813229877235584 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7847 0.7158064886895824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7548 0.6885315887127524 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7334 0.669010422843048 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7102 0.6478472897506582 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6766 0.617197234927197 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6499 0.5928413877906966 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6439 0.5873681637150786 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4779 0.4359422976229788 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4387 0.4001839003289408 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4015 0.3662499110601088 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3538 0.3227377796589452 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2980 0.2718367957556972 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2904 0.2649040452599143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2852 0.2601605843943786 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2604 0.237537924881824 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2582 0.23553107605409734 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2532 0.2309700559910823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 0.22522317071168332 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2259 0.2060668864470201 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2147 0.1958502015058664 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2089 0.19055941823276892 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2081 0.18982965502268653 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1980 0.18061639449539613 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1823 0.16629479149752885 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1813 0.16538258748492582 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.16374062026224043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1688 0.1539800373273882 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1622 0.1479594908442083 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1567 0.14294236877489178 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1546 0.14102674034842544 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1483 0.13527985506902648 RNA PCR Primer, Index 31 (95% over 22bp) TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1453 0.13254324303121745 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1415 0.129076867783326 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1355 0.12360364370770795 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1209 0.11028546512370399 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1131 0.1031702738254005 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1113 0.10152830660271508 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.122040126030107E-5 0.0 12-13 0.0 0.0 0.0 1.8244080252060213E-4 0.0 14-15 0.0 0.0 0.0 3.6488160504120426E-4 0.0 16-17 0.0 0.0 0.0 6.84153009452258E-4 0.0 18-19 0.0 0.0 0.0 0.001459526420164817 0.0 20-21 0.0 0.0 0.0 0.002736612037809032 0.0 22-23 0.0 0.0 0.0 0.006567868890741676 0.0 24-25 0.0 0.0 0.0 0.01295329697896275 0.0 26-27 0.0 0.0 0.0 0.02326120232137677 0.0 28-29 0.0 0.0 0.0 0.04971511868686408 0.0 30-31 0.0 0.0 0.0 0.10380881663422262 0.0 32-33 0.0 0.0 0.0 0.19211016505419404 0.0 34-35 0.0 0.0 0.0 0.38923745217770467 0.0 36-37 0.0 0.0 0.0 0.6644037925794029 0.0 38-39 0.0 0.0 0.0 0.9159896592553132 0.0 40-41 0.0 0.0 0.0 1.1694455441570597 0.0 42-43 0.0 0.0 0.0 1.4184772395976815 0.0 44-45 0.0 0.0 0.0 1.6904964761558994 0.0 46-47 0.0 0.0 0.0 2.049083873510143 0.0 48-49 0.0 0.0 0.0 2.471799212950378 0.0 50-51 0.0 0.0 0.0 2.8092690874128614 0.0 52-53 0.0 0.0 0.0 3.086305446040396 0.0 54-55 0.0 0.0 0.0 3.376523152650044 0.0 56-57 0.0 0.0 0.0 3.6693406406956104 0.0 58-59 0.0 0.0 0.0 4.116548657874236 0.0 60-61 0.0 4.561020063015053E-5 0.0 4.7538143810786995 0.0 62-63 0.0 9.122040126030107E-5 0.0 5.213884474835028 0.0 64-65 0.0 9.122040126030107E-5 0.0 5.5494843310716755 0.0 66-67 0.0 9.122040126030107E-5 0.0 5.942370599299792 0.0 68-69 0.0 9.122040126030107E-5 0.0 6.350581894939639 0.0 70-71 0.0 9.122040126030107E-5 0.0 6.810059056087776 0.0 72-73 0.0 9.122040126030107E-5 0.0 7.363766891737804 0.0 74-75 0.0 9.122040126030107E-5 0.0 7.889743725404699 0.0 76-77 0.0 9.122040126030107E-5 0.0 8.33882176080916 0.0 78-79 0.0 9.122040126030107E-5 0.0 8.81695349401503 0.0 80-81 0.0 9.122040126030107E-5 0.0 9.303249453133695 0.0 82-83 0.0 9.122040126030107E-5 0.0 9.794699364923567 0.0 84-85 0.0 9.122040126030107E-5 0.0 10.611213176604522 0.0 86-87 0.0 9.122040126030107E-5 0.0 11.480041888408259 0.0 88-89 0.0 9.122040126030107E-5 0.0 12.044696172209523 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCCGA 25 0.005022455 56.99644 6 CCTACGC 40 4.496601E-4 29.685648 94-95 TAGCCCT 895 0.0 22.289112 4 GTAGCCC 650 0.0 21.922709 3 GAGTACT 6320 0.0 21.907257 12-13 CATGGGG 3135 0.0 21.81682 4 ATAGGAC 240 5.9280865E-9 21.770466 3 AGTACTC 635 0.0 21.69156 5 GTGTAGC 685 0.0 21.49697 1 GTATGAC 160 4.9896626E-5 20.7809 3 GCAACGC 115 0.0037235322 20.651827 3 GTACTTT 6780 0.0 20.420923 14-15 GTCCATA 165 6.320972E-5 20.152096 1 ATGGGGA 2125 0.0 20.116392 5 GCGCACG 190 8.255129E-6 19.998753 7 AACGCCG 60 0.004766574 19.790432 94-95 TAGGACT 320 3.965397E-10 19.295671 4 TACACTG 520 0.0 19.181496 5 AGTACTT 6370 0.0 19.125572 12-13 TGTAGCC 820 0.0 19.116405 2 >>END_MODULE