##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112752 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07170870059097 33.0 31.0 34.0 31.0 34.0 2 32.2057682214905 34.0 31.0 34.0 31.0 34.0 3 32.27307881720275 34.0 31.0 34.0 30.0 34.0 4 35.71992681208391 37.0 35.0 37.0 35.0 37.0 5 35.7626038865803 37.0 35.0 37.0 35.0 37.0 6 35.821566710282255 37.0 36.0 37.0 35.0 37.0 7 35.80874175018333 37.0 36.0 37.0 35.0 37.0 8 35.82910927142795 37.0 36.0 37.0 35.0 37.0 9 37.483898478726616 39.0 38.0 39.0 35.0 39.0 10-11 37.422478683480236 39.0 37.5 39.0 35.0 39.0 12-13 37.353316821717684 39.0 37.5 39.0 35.0 39.0 14-15 38.457881900010065 40.0 38.0 41.0 34.0 41.0 16-17 38.43191025493552 40.0 38.0 41.0 34.5 41.0 18-19 38.549104382647705 40.0 38.0 41.0 35.0 41.0 20-21 38.48391465483773 40.0 38.0 41.0 34.0 41.0 22-23 38.36191712079601 40.0 38.0 41.0 34.0 41.0 24-25 38.30906617107855 40.0 38.0 41.0 34.0 41.0 26-27 38.154347958934245 40.0 38.0 41.0 34.0 41.0 28-29 38.03637153651488 40.0 38.0 41.0 34.0 41.0 30-31 37.927729628884066 40.0 37.5 41.0 34.0 41.0 32-33 37.76408759543906 40.0 37.0 41.0 34.0 41.0 34-35 37.60278301005076 40.0 37.0 41.0 33.0 41.0 36-37 37.294551256704096 40.0 36.0 41.0 33.0 41.0 38-39 36.93609133032338 39.5 35.5 41.0 32.0 41.0 40-41 36.677134258127595 39.0 35.0 41.0 31.0 41.0 42-43 36.59601959825729 39.0 35.0 41.0 31.0 41.0 44-45 36.48766481659885 39.0 35.0 41.0 31.0 41.0 46-47 36.27360319280487 39.5 35.0 41.0 30.0 41.0 48-49 36.030868513379446 40.0 35.0 41.0 29.5 41.0 50-51 35.371640311587846 38.5 35.0 40.5 27.5 40.5 52-53 35.255844069478194 38.5 35.0 40.0 27.5 41.0 54-55 35.45802838368297 39.0 35.0 41.0 28.0 41.0 56-57 35.42482197291041 39.0 35.0 41.0 27.5 41.0 58-59 35.28306217378176 39.0 35.0 41.0 26.5 41.0 60-61 34.895560286568795 38.5 35.0 40.5 25.5 41.0 62-63 34.41213720577451 38.0 34.5 40.0 24.0 41.0 64-65 33.815205005248245 37.5 34.0 40.0 22.0 41.0 66-67 33.41323089062073 37.0 33.5 39.5 21.5 41.0 68-69 33.03803273325953 36.0 33.0 39.0 20.5 41.0 70-71 32.488518106460376 36.0 33.0 38.5 18.0 40.0 72-73 31.83816474829971 35.0 32.0 37.5 12.5 40.0 74-75 31.32276284383223 35.0 31.0 37.0 7.5 39.0 76-77 30.650264389549513 35.0 30.5 36.5 4.5 39.0 78-79 30.12069176240528 34.0 30.0 36.0 2.0 38.5 80-81 29.722236401282586 34.0 30.0 35.5 2.0 37.0 82-83 29.259441007520095 34.0 29.0 35.0 2.0 37.0 84-85 28.80768625893281 34.0 29.0 35.0 2.0 36.0 86-87 28.187586272592632 34.0 28.0 35.0 2.0 36.0 88-89 27.484953071304297 33.5 26.0 35.0 2.0 35.5 90-91 26.794627194559077 33.0 25.0 35.0 2.0 35.0 92-93 26.38669263232059 33.0 25.0 35.0 2.0 35.0 94-95 25.95621845658332 33.0 24.5 35.0 2.0 35.0 96-97 25.036229995542584 32.0 18.5 35.0 2.0 35.0 98-99 23.948136691733648 32.0 2.0 34.0 2.0 35.0 100-101 21.206070175564726 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3701597628769022 1101 2 0.13711953456659387 1101 3 0.33889742027014336 1101 4 0.09838720303808657 1101 5 -0.0010581140991661186 1101 6 0.15513287921513808 1101 7 0.29526607176283903 1101 8 0.13026563486746312 1101 9 0.235093565630919 1101 10-11 0.28245083321055375 1101 12-13 0.14500347553504866 1101 14-15 0.3323359109937911 1101 16-17 0.46516581102931553 1101 18-19 0.2585157064953876 1101 20-21 0.45436789430505087 1101 22-23 0.2683165393123943 1101 24-25 0.4682563062019369 1101 26-27 0.42749359612350446 1101 28-29 0.22123265659456592 1101 30-31 0.19007024383833482 1101 32-33 0.17136663537490193 1101 34-35 0.1807282640092609 1101 36-37 0.2769030476652148 1101 38-39 0.4141130975784151 1101 40-41 0.3316593280748279 1101 42-43 0.4240522295366844 1101 44-45 0.27872324106515833 1101 46-47 0.2262024025851872 1101 48-49 0.2922760224507357 1101 50-51 0.46087937794435874 1101 52-53 0.20486103630212682 1101 54-55 0.4047714975196257 1101 56-57 0.2958227280303376 1101 58-59 0.22359261360280414 1101 60-61 0.18043514184949316 1101 62-63 0.3061270129393847 1101 64-65 0.11517557273777612 1101 66-67 0.08452243381489666 1101 68-69 0.2533007650557906 1101 70-71 0.3046107926469901 1101 72-73 0.405925799028509 1101 74-75 0.5414528071480191 1101 76-77 0.41934727842754427 1101 78-79 0.31645411097035137 1101 80-81 0.18103853004894432 1101 82-83 0.29027994461469575 1101 84-85 0.4199578505364805 1101 86-87 0.5664940556711784 1101 88-89 0.3867331014241948 1101 90-91 0.4506732746387705 1101 92-93 0.4979958040128274 1101 94-95 0.4738864020701463 1101 96-97 0.4122599462166967 1101 98-99 0.5635840314083147 1101 100-101 0.5958041813531345 1102 1 0.22432382539604845 1102 2 0.20708470639130638 1102 3 0.10996650554648113 1102 4 0.16383892765775698 1102 5 0.01833512565642792 1102 6 0.21227951784279497 1102 7 0.02713323940621848 1102 8 0.12599483293708857 1102 9 0.18284149885690226 1102 10-11 0.26937249800223384 1102 12-13 0.07180609708439789 1102 14-15 0.33079119195985385 1102 16-17 0.26419241132392557 1102 18-19 0.2837146633657994 1102 20-21 0.34740544549952546 1102 22-23 0.23412438876448505 1102 24-25 0.21242546672281293 1102 26-27 0.20804913125437707 1102 28-29 0.23136064584954852 1102 30-31 0.2221318953457967 1102 32-33 0.3118392544487776 1102 34-35 0.12937998960931907 1102 36-37 0.3351385263259061 1102 38-39 0.3675919935669256 1102 40-41 0.3090136612537506 1102 42-43 0.33008049403474615 1102 44-45 0.27696406282382924 1102 46-47 0.31975624900298527 1102 48-49 0.3265674369746492 1102 50-51 0.36235620532062995 1102 52-53 0.41620935972132145 1102 54-55 0.4689070553464063 1102 56-57 0.4084720222757241 1102 58-59 0.32932590477958 1102 60-61 0.31841084300855726 1102 62-63 0.22504305032146021 1102 64-65 0.1545570233643616 1102 66-67 0.17719025733580196 1102 68-69 0.27612841583286496 1102 70-71 0.28312136732413506 1102 72-73 0.4330401829913768 1102 74-75 0.15077553271621724 1102 76-77 0.25850532383407554 1102 78-79 0.09874125101589115 1102 80-81 0.15259511174074447 1102 82-83 0.18488488624502608 1102 84-85 -0.0552494396059906 1102 86-87 -0.1939519459513548 1102 88-89 0.06458718583043677 1102 90-91 0.19172052184038435 1102 92-93 0.27055507441034976 1102 94-95 0.1938700786607619 1102 96-97 0.1986463208266862 1102 98-99 -0.2842755431536368 1102 100-101 -0.31531779569114704 1103 1 0.41129631530013455 1103 2 0.31874359655182616 1103 3 0.3779455846004751 1103 4 0.32462298909328524 1103 5 0.27136389985783893 1103 6 0.365499262267754 1103 7 0.2790728512806808 1103 8 0.27300578776563356 1103 9 0.3956389868986889 1103 10-11 0.29816419373204184 1103 12-13 0.22878655536779036 1103 14-15 0.40695354825250973 1103 16-17 0.48627260978849307 1103 18-19 0.48605732173452054 1103 20-21 0.3661604821698745 1103 22-23 0.38949819153242515 1103 24-25 0.4623865277587953 1103 26-27 0.3360996322690397 1103 28-29 0.400974822792719 1103 30-31 0.4427933611212751 1103 32-33 0.5246045755202928 1103 34-35 0.4711596921382011 1103 36-37 0.2878401003629847 1103 38-39 0.3807810157136018 1103 40-41 0.46780111320081375 1103 42-43 0.29512007633817205 1103 44-45 0.3674480572166132 1103 46-47 0.376941785830212 1103 48-49 0.29326390041378403 1103 50-51 0.09324563897879301 1103 52-53 0.37369196134073235 1103 54-55 0.5579266060923445 1103 56-57 0.5785670107746199 1103 58-59 0.46473925599098465 1103 60-61 0.441238443849187 1103 62-63 0.3102685915730561 1103 64-65 0.5114049763035169 1103 66-67 0.542760538215525 1103 68-69 0.4872729730686203 1103 70-71 0.5342314099651233 1103 72-73 0.5921419683146638 1103 74-75 0.4139402885519843 1103 76-77 0.4030777965824406 1103 78-79 0.5086421316008085 1103 80-81 0.42358603501179815 1103 82-83 0.47444292336006555 1103 84-85 0.5440116230101175 1103 86-87 0.429512222301085 1103 88-89 0.3694053465117868 1103 90-91 0.6310665955512782 1103 92-93 0.6489627980949457 1103 94-95 0.5478388055936563 1103 96-97 0.2648854489550665 1103 98-99 0.7324055704330128 1103 100-101 0.3936540802188375 1104 1 0.35821084116202684 1104 2 0.44876835815553306 1104 3 0.2615275471298091 1104 4 0.3558181518673962 1104 5 0.16506731987704626 1104 6 0.2579868915974899 1104 7 0.2905863891100111 1104 8 0.24261102052876282 1104 9 0.32037951690237776 1104 10-11 0.4177524054255173 1104 12-13 0.2983571380962644 1104 14-15 0.3803956137998412 1104 16-17 0.4769728241729112 1104 18-19 0.4796004600145025 1104 20-21 0.43092767851601366 1104 22-23 0.42965778432359514 1104 24-25 0.5516083406648136 1104 26-27 0.4787280906869782 1104 28-29 0.7122770156738554 1104 30-31 0.5815079866780053 1104 32-33 0.631894051230816 1104 34-35 0.7872906003743765 1104 36-37 0.8076433043767111 1104 38-39 0.8469749424338389 1104 40-41 0.9004442826860881 1104 42-43 1.0203564463113821 1104 44-45 0.9649017862492926 1104 46-47 1.0119259602880675 1104 48-49 0.7940592301564848 1104 50-51 0.8965914949562332 1104 52-53 0.9145171995343944 1104 54-55 0.9615701281569144 1104 56-57 0.9142437806619128 1104 58-59 1.1782918774383404 1104 60-61 1.0369677764206315 1104 62-63 0.8375955136368276 1104 64-65 0.8433249529603373 1104 66-67 0.7398716169055533 1104 68-69 0.7183076514717257 1104 70-71 0.8444089964585046 1104 72-73 0.8283326124000396 1104 74-75 0.704946882190157 1104 76-77 0.6663825993609258 1104 78-79 0.5824831791260685 1104 80-81 0.6383625524198955 1104 82-83 0.7914735167064499 1104 84-85 0.9456268009410884 1104 86-87 0.8199218627107285 1104 88-89 0.8538037124890998 1104 90-91 0.8736928250196137 1104 92-93 0.6555088177988608 1104 94-95 0.7242956267873204 1104 96-97 0.8284854862393125 1104 98-99 0.9066496525718328 1104 100-101 0.7604500259621503 1105 1 0.27961110414472756 1105 2 0.23610277218914888 1105 3 0.211432663120668 1105 4 0.05078773181431728 1105 5 0.1355619892214932 1105 6 0.11831928992221918 1105 7 0.24578619878872843 1105 8 0.12668101892430883 1105 9 0.14450753378048375 1105 10-11 0.21020539219707501 1105 12-13 0.17052237458705122 1105 14-15 0.19764799788735843 1105 16-17 0.2647738204089052 1105 18-19 0.18225158746520975 1105 20-21 0.2493774084331548 1105 22-23 0.30537679118585714 1105 24-25 0.3198834249907847 1105 26-27 0.279364707984449 1105 28-29 0.3695788427212392 1105 30-31 0.35304960747023273 1105 32-33 0.46685719492522537 1105 34-35 0.45829574384379157 1105 36-37 0.5845409384545519 1105 38-39 0.46127743892525785 1105 40-41 0.5141842986568435 1105 42-43 0.44680658564057296 1105 44-45 0.507833803562626 1105 46-47 0.6950059929672676 1105 48-49 0.7741949078348611 1105 50-51 0.6858148292301038 1105 52-53 0.7797754518613047 1105 54-55 0.7003053031068021 1105 56-57 0.7733534754286353 1105 58-59 0.6905622516815271 1105 60-61 0.8018956405990991 1105 62-63 0.6956392617119391 1105 64-65 0.7682158225183358 1105 66-67 0.7723004631196915 1105 68-69 0.7754699649080052 1105 70-71 0.8168045733329876 1105 72-73 0.775429014184482 1105 74-75 0.803159669493219 1105 76-77 0.8033678144221277 1105 78-79 0.9124720586095414 1105 80-81 0.9136488181209401 1105 82-83 1.0562409356216556 1105 84-85 1.08068575074385 1105 86-87 0.9179631395599515 1105 88-89 0.9069498227979871 1105 90-91 0.7698183485414454 1105 92-93 0.7842002114780549 1105 94-95 1.0155798528049012 1105 96-97 0.9633554057031688 1105 98-99 0.6942975803382687 1105 100-101 0.5372148777531436 1106 1 -0.12278241009097712 1106 2 -0.07114999389629162 1106 3 -0.14107862943298244 1106 4 0.0015053314100086368 1106 5 -0.1886527782734433 1106 6 0.001287688259509423 1106 7 0.04746394961632916 1106 8 0.0341223002684643 1106 9 -0.03264697003791639 1106 10-11 -0.5463410537306927 1106 12-13 -0.4075129746470765 1106 14-15 -0.3263212450962172 1106 16-17 -0.13348521144807535 1106 18-19 -0.14216253753894392 1106 20-21 -0.09782205574981617 1106 22-23 -0.1662947690664538 1106 24-25 -0.03754141810261302 1106 26-27 -0.031847228284100026 1106 28-29 -0.1664419114447213 1106 30-31 -0.12844992823661983 1106 32-33 -0.15016610017460152 1106 34-35 -0.361354785940037 1106 36-37 -0.589958962741072 1106 38-39 -0.3215960022826394 1106 40-41 -0.10170230889041676 1106 42-43 -0.10392246826813789 1106 44-45 -0.10877503776803366 1106 46-47 0.16909826051903565 1106 48-49 0.04196308303163221 1106 50-51 0.3302480330976394 1106 52-53 0.16941751769474678 1106 54-55 -0.24283889707611905 1106 56-57 -0.2374489910084847 1106 58-59 0.05947988030646911 1106 60-61 0.09700027706018943 1106 62-63 0.1118583940870792 1106 64-65 0.13117720836780222 1106 66-67 -0.042609205194885647 1106 68-69 -0.43779360495543784 1106 70-71 -0.14782345878105474 1106 72-73 -0.04042803948640028 1106 74-75 -0.0634058902729393 1106 76-77 0.22964444569992182 1106 78-79 0.3600069330580311 1106 80-81 0.32456923775100677 1106 82-83 0.21469255732504422 1106 84-85 0.15033759209024566 1106 86-87 0.30034220911998233 1106 88-89 0.05845234886747974 1106 90-91 -0.3001351521939064 1106 92-93 -0.5082410786098528 1106 94-95 -0.4985832741001417 1106 96-97 -0.35690237754625187 1106 98-99 -0.3230338445258063 1106 100-101 -0.6002242001659148 1107 1 0.2113714466418699 1107 2 0.24321634210517118 1107 3 0.19392904097365715 1107 4 0.16092781609784623 1107 5 0.18634578915327893 1107 6 0.2325361226615641 1107 7 0.04953831572311884 1107 8 0.18731186327631377 1107 9 0.06641915590737568 1107 10-11 0.16032073945811476 1107 12-13 0.0782366049949843 1107 14-15 0.16950628290444314 1107 16-17 0.2779723459080614 1107 18-19 0.29671897473452447 1107 20-21 0.22394863756652938 1107 22-23 0.2930082799406506 1107 24-25 0.3622279785302709 1107 26-27 0.32251246057735017 1107 28-29 0.2224517981669436 1107 30-31 0.21798353733004916 1107 32-33 0.19404646750425059 1107 34-35 0.34780664296584973 1107 36-37 0.3833632483662015 1107 38-39 0.3488957714722929 1107 40-41 0.4783462021338565 1107 42-43 0.34549582511959187 1107 44-45 0.32592786290634024 1107 46-47 0.2950656567884522 1107 48-49 0.2938835498376804 1107 50-51 0.3311620148491201 1107 52-53 0.40594641720014835 1107 54-55 0.44750793300759284 1107 56-57 0.5258935871977428 1107 58-59 0.5422419307035824 1107 60-61 0.5743963172639255 1107 62-63 0.3192401576748338 1107 64-65 0.5556965595676431 1107 66-67 0.5717256960558004 1107 68-69 0.5348115008994441 1107 70-71 0.580635141488429 1107 72-73 0.744639717136593 1107 74-75 0.6903900196075625 1107 76-77 0.8222218333084079 1107 78-79 0.7357938250944009 1107 80-81 0.7794006115969694 1107 82-83 0.8396604267529497 1107 84-85 0.7595929978031855 1107 86-87 0.7875595624121985 1107 88-89 0.8354051918296115 1107 90-91 0.5150563460157862 1107 92-93 0.6774740374122068 1107 94-95 0.729650207511348 1107 96-97 0.6128199815663038 1107 98-99 0.3949641056664852 1107 100-101 0.5120048183658561 1108 1 0.29930776613880994 1108 2 0.23719083960639864 1108 3 0.22346992488921558 1108 4 0.18796760636460164 1108 5 0.13890461818389355 1108 6 0.27964881254295904 1108 7 0.26006068567165386 1108 8 0.1904080873668832 1108 9 0.1996340814696964 1108 10-11 0.20125566619523738 1108 12-13 0.13271615955917326 1108 14-15 0.29905497307644424 1108 16-17 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-0.45125226267556684 2316 56-57 -0.43265085318297025 2316 58-59 -0.6018739099372539 2316 60-61 -0.5092503182834136 2316 62-63 -0.48637842425203104 2316 64-65 -0.38271102740152685 2316 66-67 -0.57027112961066 2316 68-69 -0.3704025181685111 2316 70-71 -0.6911355345799031 2316 72-73 -0.7141728140275205 2316 74-75 -0.8958225451834565 2316 76-77 -0.7358596882454513 2316 78-79 -0.558623011006798 2316 80-81 -0.584716072605378 2316 82-83 -0.5911392175645389 2316 84-85 -0.7279006249295286 2316 86-87 -0.7446211538870848 2316 88-89 -0.8751076069052743 2316 90-91 -0.6517417660242053 2316 92-93 -0.7182556924112156 2316 94-95 -0.8276807754053763 2316 96-97 -0.901915478119836 2316 98-99 -0.9818730239825655 2316 100-101 -0.8315789429593927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5437.0 3 895.0 4 687.0 5 711.0 6 734.0 7 796.0 8 971.0 9 1161.0 10 1582.0 11 2130.0 12 3073.0 13 4468.0 14 5278.0 15 6740.0 16 8879.0 17 12120.0 18 10604.0 19 7980.0 20 7642.0 21 7560.0 22 8005.0 23 8675.0 24 9822.0 25 11022.0 26 12534.0 27 14798.0 28 17221.0 29 20584.0 30 24940.0 31 30637.0 32 38888.0 33 50797.0 34 71810.0 35 119339.0 36 157924.0 37 215895.0 38 184671.0 39 25741.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.71363229501519 17.37842356407438 13.700036941930719 19.207907198979715 2 13.448492580914026 22.85364399716238 40.87389037393488 22.823973047988712 3 23.347983951458737 28.727230547617804 30.070513592884257 17.854271908039195 4 11.69840334887569 16.210123064886712 39.60036510298872 32.49110848324888 5 12.344392089213553 41.12824673134571 33.36361322445764 13.163747954983096 6 25.981038680156832 37.71049889971085 18.90617043595921 17.40229198417311 7 24.8530777898583 38.176662772722246 19.564687589648404 17.405571847771053 8 21.304472531197487 32.44232769790027 21.08418623093737 25.16901353996488 9 25.132979679913685 21.899388599172813 21.671731702931126 31.295900017982376 10-11 22.61016078778234 25.72721431131324 27.491621561220274 24.171003339684145 12-13 31.550492312245932 24.0930042585043 25.119552548917472 19.236950880332294 14-15 26.42052913440201 25.80011257413528 29.32412359417204 18.455234697290667 16-17 18.832140745586525 29.35348480658947 31.5538260597845 20.260548388039503 18-19 17.54044422783469 32.99777805153734 31.735241937006727 17.726535783621244 20-21 20.31762705599372 30.06961497938441 28.031170027201153 21.581587937420714 22-23 18.89198982580948 29.286588170547635 26.60644344417122 25.214978559471668 24-25 20.668110659763325 29.193321771793922 29.72006742632217 20.418500142120582 26-27 23.95520368053913 25.96795355441628 30.81520776323256 19.26163500181203 28-29 19.451351387808508 27.08013902815185 33.86643944946291 19.60207013457673 30-31 18.78378062401862 32.871593609088144 30.868048498409106 17.476577268484128 32-33 18.41587087891211 31.357162674350477 28.85403415715746 21.372932289579957 34-35 20.139414122537296 27.829359344352522 29.215901231992042 22.815325301118136 36-37 22.559977337772853 27.84229967647872 31.048420276398236 18.549302709350194 38-39 24.88240815159639 24.493581894451427 32.41390532597575 18.21010462797643 40-41 19.798052358760497 27.083028962880817 35.12276936728856 17.996149311070123 42-43 17.97255406834152 30.99539151448895 31.75278882599596 19.279265591173573 44-45 18.696967245532665 32.35910365656348 28.426275482687952 20.517653615215906 46-47 18.465703257170286 29.08472202495779 26.27081239583689 26.178762322035027 48-49 20.846285366015906 28.115005060956204 28.665507650763463 22.373201922264432 50-51 24.490050914007107 29.195461605948598 28.563036646579125 17.751450833465174 52-53 19.502019114735607 28.855385541925 32.81523246224132 18.827362881098075 54-55 17.24719257669908 28.412391224425775 33.47595544077871 20.864460758096428 56-57 20.173063629850756 32.34109166808242 27.514762049868718 19.971082652198106 58-59 18.439984054412744 29.359070985278198 29.11026948851428 23.090675471794782 60-61 19.413076317697286 26.21169361816117 30.747221364514637 23.62800869962691 62-63 25.514678123932622 25.4113559961889 28.804582307153005 20.269383572725474 64-65 23.11227583057796 26.394813233158672 31.83654098093209 18.656369955331282 66-67 18.69611095581739 27.537981299439956 33.23065971470096 20.535248030041693 68-69 18.05530712749348 31.850809535922398 30.971344235005855 19.122539101578266 70-71 18.96891191244018 30.068165614933502 27.809134480095587 23.15378799253073 72-73 18.588015301145788 28.66245330768159 26.79359185856779 25.955939532604837 74-75 20.545237899396223 28.357216089161092 29.05309042006412 22.044455591378565 76-77 23.14183525706309 25.286626894703772 30.39256311634333 21.17897473188981 78-79 19.898615171322835 25.804955543022267 32.81444014767311 21.481989137981785 80-81 19.180886390366076 30.98937278918107 30.075676239260773 19.75406458119208 82-83 19.198878577422068 29.78331537852731 28.305043839838635 22.712762204211987 84-85 20.314735143312078 26.525756715738417 28.611002876713005 24.548505264236496 86-87 22.331275937455764 26.580491213563985 30.076820930164626 21.01141191881562 88-89 24.665826362766747 23.744713637377103 31.00532592043844 20.58413407941771 90-91 20.572805560363527 25.768112879695547 33.222722501084604 20.436359058856322 92-93 19.223136625093773 28.96083050494726 31.141013867969097 20.675019001989874 94-95 19.511862870584046 30.085334980598326 28.526457620466367 21.876344528351254 96-97 19.55740142698908 27.27507300372571 26.60104722585842 26.566478343426787 98-99 21.58635553547871 26.503504833051284 28.06095869937994 23.849180932090068 100-101 24.63690620864728 26.429330164645727 28.74467759503812 20.18908603166887 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6801.0 1 4009.0 2 1699.5 3 2128.5 4 1496.0 5 1439.5 6 3407.0 7 6714.5 8 8936.5 9 8592.0 10 6157.0 11 3981.0 12 3560.5 13 3374.0 14 2900.0 15 2341.5 16 2023.0 17 1974.0 18 2033.0 19 2174.5 20 2346.0 21 2666.5 22 3141.0 23 3641.0 24 4278.5 25 5125.0 26 6211.5 27 7609.0 28 9145.5 29 10614.0 30 11983.0 31 13617.0 32 15359.0 33 17160.0 34 18953.5 35 20548.5 36 21864.0 37 22874.5 38 23454.0 39 23964.5 40 24825.0 41 25675.5 42 25830.0 43 25824.0 44 26915.5 45 28799.0 46 36131.0 47 53822.5 48 157947.0 49 163396.0 50 58602.0 51 35291.5 52 26226.0 53 20707.5 54 16992.0 55 14516.5 56 12320.5 57 9850.5 58 7783.0 59 6098.5 60 4495.0 61 3280.5 62 2456.5 63 1872.0 64 1394.5 65 1038.0 66 823.0 67 686.0 68 515.0 69 337.5 70 289.5 71 263.0 72 201.0 73 162.5 74 132.0 75 96.5 76 62.5 77 48.5 78 40.5 79 29.5 80 18.0 81 13.0 82 14.5 83 9.5 84 6.0 85 6.0 86 4.0 87 0.5 88 1.0 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5040655959279337 2 0.04969660804923289 3 0.056256919780867616 4 0.0662321883043122 5 0.08115015744748155 6 0.11017729017786533 7 0.06910794139215207 8 0.05059528088918286 9 0.0496067407652379 10-11 0.07373610651789438 12-13 0.10887421455993788 14-15 0.05356090126101773 16-17 0.060615483054624934 18-19 0.05976174385667247 20-21 0.07643212503774426 22-23 0.06492911268638474 24-25 0.09166462967489611 26-27 0.06659165744029218 28-29 0.06713086114426214 30-31 0.07171409262800696 32-33 0.07220836268997945 34-35 0.0730621018879319 36-37 0.06915287503414957 38-39 0.03864293211784836 40-41 0.04345083181158066 42-43 0.04097948150171826 44-45 0.020849209886839117 46-47 0.028218327174428803 48-49 0.028218327174428803 50-51 0.0258817777905589 52-53 0.02332056019670151 54-55 0.022916157418724027 56-57 0.02435403396264397 58-59 0.019636001552906665 60-61 0.026106446000546395 62-63 0.01905186420693919 64-65 0.02053467439285663 66-67 0.01482810185917437 68-69 0.018063324082994235 70-71 0.04142881792169324 72-73 0.03747465742591341 74-75 0.03042007563230621 76-77 0.021837750010784075 78-79 0.014333831797201893 80-81 0.016490646613081802 82-83 0.0212536126648166 84-85 0.017748788589011747 86-87 0.00894179475750212 88-89 0.04097948150171826 90-91 0.053426100335025234 92-93 0.0331160941521561 94-95 0.054414640458970195 96-97 0.04313629631759817 98-99 0.032307288596201134 100-101 0.16913022847858283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1112752.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.38118625645573 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75370159990739 28.651270735525536 2 14.839275736904915 11.687765634119133 3 5.633882608393483 6.656069410444511 4 2.6645770716567156 4.197368238143779 5 1.4483729945405819 2.8519323333411593 6 0.8423562218288096 1.990379235967481 7 0.5017189788641077 1.383080198853234 8 0.32152847749851676 1.0129738287298977 9 0.21545244270168637 0.763629549789912 >10 0.6700662087253487 4.179368503708852 >50 0.048017912356479 1.3191917463038232 >100 0.04458689360896677 3.595331627990222 >500 0.005487617671017648 1.482236874024019 >1k 0.006630871352479658 5.716440417382955 >5k 0.002057856626631618 6.145146435074976 >10k+ 0.00228650736292402 18.36781523060054 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 47292 4.25000359469136 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35578 3.1972982299739745 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28499 2.5611277265733965 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16405 1.4742727939379126 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15155 1.3619386889441671 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14497 1.3028060160754598 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13072 1.1747451363825903 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12212 1.0974592721468934 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 11250 1.0110069449437071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10024 0.9008296547658418 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 9130 0.8204883028743152 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9074 0.8154557349705955 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7807 0.7015938861489353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7737 0.6953031762692855 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7404 0.6653773706989519 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7089 0.637069176240528 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7010 0.6299696608049233 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6646 0.5972579694307447 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6348 0.5704775188002358 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4509 0.40521158353343784 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4404 0.3957755187139632 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4054 0.36432196931571453 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3408 0.306267703854947 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2983 0.2680741081570736 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2973 0.2671754353171237 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2666 0.23958617913065983 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2662 0.23922670999467988 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2597 0.2333853365350051 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2546 0.2288021050512603 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2177 0.1956410772571067 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2104 0.18908076552547198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.18099270996592232 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2002 0.17991430255798235 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1987 0.17856629329805743 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1809 0.1625699167469481 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1795 0.16131177477101816 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1782 0.1601435000790832 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1764 0.15852588896717326 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1614 0.14504579636792383 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1523 0.1368678735243792 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1505 0.13525026241246926 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1505 0.13525026241246926 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1434 0.12886968524882453 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.11835521302140997 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1202 0.10802047536198542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.986728399499619E-5 0.0 9 0.0 0.0 0.0 8.986728399499619E-5 0.0 10-11 0.0 0.0 0.0 1.348009259924943E-4 0.0 12-13 0.0 0.0 0.0 1.7973456798999238E-4 0.0 14-15 0.0 0.0 0.0 2.2466820998749047E-4 0.0 16-17 0.0 0.0 0.0 5.392037039699772E-4 0.0 18-19 0.0 0.0 0.0 0.0010334737659424562 0.0 20-21 0.0 0.0 0.0 0.002022013889887414 0.0 22-23 0.0 0.0 0.0 0.00660524537363222 0.0 24-25 0.0 0.0 0.0 0.013255424389261937 0.0 26-27 0.0 0.0 0.0 0.024533768530633958 0.0 28-29 0.0 0.0 0.0 0.046101916689433045 0.0 30-31 0.0 0.0 0.0 0.09961788430845328 0.0 32-33 0.0 0.0 0.0 0.19056357571138943 0.0 34-35 0.0 0.0 0.0 0.38521611284455115 0.0 36-37 0.0 0.0 0.0 0.6568399787194271 0.0 38-39 0.0 0.0 0.0 0.909591714955354 0.0 40-41 0.0 0.0 0.0 1.1597372999554258 0.0 42-43 0.0 0.0 0.0 1.4094335485355227 0.0 44-45 0.0 0.0 0.0 1.6810574144103987 0.0 46-47 0.0 0.0 0.0 2.0398076121184237 0.0 48-49 0.0 0.0 0.0 2.4568367434972034 0.0 50-51 0.0 0.0 0.0 2.790873438106604 0.0 52-53 0.0 0.0 0.0 3.0641149150933904 0.0 54-55 0.0 0.0 0.0 3.3460735186276906 0.0 56-57 0.0 0.0 0.0 3.636389779573526 0.0 58-59 0.0 0.0 0.0 4.094937596157994 0.0 60-61 0.0 0.0 0.0 4.729400621162666 0.0 62-63 0.0 0.0 0.0 5.200215322012452 0.0 64-65 0.0 0.0 0.0 5.530163055200081 0.0 66-67 0.0 0.0 0.0 5.917491049218514 0.0 68-69 0.0 0.0 0.0 6.326207456827756 0.0 70-71 0.0 0.0 0.0 6.7798125727925 0.0 72-73 0.0 0.0 0.0 7.325756323062102 0.0 74-75 0.0 0.0 0.0 7.846851769307087 0.0 76-77 0.0 0.0 0.0 8.288459602858499 0.0 78-79 0.0 0.0 0.0 8.753208262038623 0.0 80-81 0.0 4.4933641997498096E-5 0.0 9.241232547773448 0.0 82-83 0.0 8.986728399499619E-5 0.0 9.716046342761011 0.0 84-85 0.0 8.986728399499619E-5 0.0 10.52655937711188 0.0 86-87 0.0 8.986728399499619E-5 0.0 11.409415575078723 0.0 88-89 0.0 8.986728399499619E-5 0.0 11.981960041410844 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGT 55 0.0025722615 34.5655 4 ACGACGA 30 0.0039331615 31.697165 94-95 AATGGCG 60 0.0039396933 31.685041 5 TACACGG 60 0.0039396933 31.685041 2 AACGACG 30 0.003957053 31.657976 92-93 ATAGCCC 340 0.0 29.355257 3 CCGACGC 35 0.0084007885 27.137848 86-87 GTATAGC 430 0.0 25.547018 1 TACCACG 75 1.0519925E-6 25.357733 94-95 TAGCCCT 805 0.0 23.616179 4 GCCGTAT 485 0.0 23.037628 94-95 GTAATAC 150 3.0195431E-5 22.179527 3 TATAGCC 500 0.0 21.862679 2 GAGTACT 6460 0.0 21.48788 12-13 AGTACTT 6545 0.0 20.845654 12-13 CCGTATC 370 0.0 20.560324 94-95 AGTACTC 395 0.0 20.4549 5 GCGTTGT 280 0.0 20.376749 94-95 CATGGGG 3105 0.0 20.35802 4 GTATAGG 235 1.06800144E-7 20.324175 1 >>END_MODULE