##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112752 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.475566882827444 33.0 31.0 34.0 31.0 34.0 2 32.66736793103944 34.0 31.0 34.0 31.0 34.0 3 32.73314628955958 34.0 31.0 34.0 31.0 34.0 4 36.11688228823673 37.0 35.0 37.0 35.0 37.0 5 36.112223568234434 37.0 35.0 37.0 35.0 37.0 6 36.18918231555639 37.0 36.0 37.0 35.0 37.0 7 36.17905247530447 37.0 36.0 37.0 35.0 37.0 8 36.21486458797648 37.0 37.0 37.0 35.0 37.0 9 37.87665355802551 39.0 38.0 39.0 35.0 39.0 10-11 37.882818453707564 39.0 38.0 39.0 35.0 39.0 12-13 37.84091918055416 39.0 38.0 39.0 35.0 39.0 14-15 38.94691899003551 40.0 38.0 41.0 35.5 41.0 16-17 38.929774109594945 40.0 38.0 41.0 35.5 41.0 18-19 39.09791804463168 40.0 38.5 41.0 36.0 41.0 20-21 39.08925528779099 40.0 39.0 41.0 35.0 41.0 22-23 38.98159697758351 40.0 38.5 41.0 35.0 41.0 24-25 38.92690284987131 40.0 38.0 41.0 35.0 41.0 26-27 38.67110461270795 40.0 38.0 41.0 34.5 41.0 28-29 38.53371595827282 40.0 38.0 41.0 34.5 41.0 30-31 38.45209444692078 40.0 38.0 41.0 35.0 41.0 32-33 38.323976501502585 40.0 37.5 41.0 34.0 41.0 34-35 38.175141451105006 40.0 37.0 41.0 34.0 41.0 36-37 37.943823062101885 40.0 37.0 41.0 34.0 41.0 38-39 37.78902396940199 40.0 37.0 41.0 33.0 41.0 40-41 37.65449534128 40.0 37.0 41.0 33.0 41.0 42-43 37.658486347362214 40.0 37.0 41.0 33.0 41.0 44-45 37.54134479201116 40.0 37.0 41.0 33.0 41.0 46-47 37.20198480883431 40.0 37.0 41.0 33.0 41.0 48-49 36.906371320833394 40.0 36.5 41.0 31.5 41.0 50-51 36.65382717802349 40.0 36.0 41.0 31.0 41.0 52-53 35.98974614289617 39.0 35.0 41.0 29.0 41.0 54-55 35.90532256962918 39.0 35.0 41.0 28.5 41.0 56-57 35.875915298287495 39.0 35.0 41.0 29.0 41.0 58-59 35.6853517225761 39.0 35.0 41.0 28.0 41.0 60-61 35.32074172861518 39.0 35.0 40.5 27.5 41.0 62-63 34.85175223230333 38.0 35.0 40.0 26.5 41.0 64-65 34.24347518584554 37.5 34.0 40.0 26.0 41.0 66-67 33.8310584928178 37.0 34.0 39.5 24.5 41.0 68-69 33.45861881173882 36.0 34.0 39.0 24.0 40.5 70-71 32.90928436884409 36.0 33.0 38.5 22.5 40.0 72-73 32.39660454440882 35.0 33.0 37.5 21.5 39.5 74-75 31.953587142507942 35.0 32.0 37.0 20.0 39.0 76-77 30.40928257149841 33.5 30.5 35.5 18.0 38.0 78-79 31.044470376148503 34.5 31.0 36.0 18.0 38.0 80-81 31.018419647864036 35.0 32.0 36.0 18.0 37.0 82-83 30.725150797302543 35.0 31.5 35.0 13.0 37.0 84-85 30.416333558600655 34.5 31.0 35.0 7.0 36.5 86-87 29.923670773002428 34.0 31.0 35.0 3.5 36.0 88-89 29.32452828662631 34.0 29.5 35.0 2.0 36.0 90-91 28.93071232403986 34.0 29.0 35.0 2.0 35.0 92-93 28.780673052036754 34.0 29.0 35.0 2.0 35.0 94-95 28.561105259752395 34.0 29.0 35.0 2.0 35.0 96-97 28.001453153982197 34.0 29.0 35.0 2.0 35.0 98-99 27.399352236616963 34.0 27.5 35.0 2.0 35.0 100-101 25.144675093821444 31.0 22.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12545295138018986 1101 2 0.13885740432792204 1101 3 0.12402270132275106 1101 4 0.0689243997356428 1101 5 0.0451932267850097 1101 6 0.03758389674391793 1101 7 0.10720110700970764 1101 8 0.11005092567208408 1101 9 0.061067665062566334 1101 10-11 0.1446765366778351 1101 12-13 0.16865305777950113 1101 14-15 0.24568257724547493 1101 16-17 0.11914662900448292 1101 18-19 0.1840725000309007 1101 20-21 0.24678005398953218 1101 22-23 0.2912786536433387 1101 24-25 0.2665741436210993 1101 26-27 0.14980661565085285 1101 28-29 0.19642594017635417 1101 30-31 0.1767921183572838 1101 32-33 0.15973701700763598 1101 34-35 0.012139087196040066 1101 36-37 0.27165940254416654 1101 38-39 0.35240490426993176 1101 40-41 0.34198876359149466 1101 42-43 0.21136260054591105 1101 44-45 0.2260939835345397 1101 46-47 0.0760310249550642 1101 48-49 0.09042043494425656 1101 50-51 0.11863054579391275 1101 52-53 0.18949883701529302 1101 54-55 0.24206961735165322 1101 56-57 -0.007800952964153396 1101 58-59 0.01588405381995983 1101 60-61 -0.12114746431382173 1101 62-63 -0.2701156135834779 1101 64-65 -0.04700960491574335 1101 66-67 -0.10459236485456103 1101 68-69 -0.2615978531579657 1101 70-71 -0.19887838699755633 1101 72-73 -0.3502960765532137 1101 74-75 -0.061219707995949335 1101 76-77 -0.09426047586778452 1101 78-79 0.04831926724085989 1101 80-81 0.029924431717962108 1101 82-83 0.00883125510944538 1101 84-85 0.19864414745423176 1101 86-87 0.12668077304934044 1101 88-89 0.0960680165465817 1101 90-91 0.2139082827879868 1101 92-93 0.18238385855605088 1101 94-95 0.053760703681536626 1101 96-97 -0.17155406883219726 1101 98-99 0.07217337976824112 1101 100-101 0.1837265928919436 1102 1 0.20931140340137944 1102 2 0.16843561821360709 1102 3 0.22211756827226026 1102 4 0.06041833482925796 1102 5 0.0012290892664097441 1102 6 0.07300765588293956 1102 7 0.07299057424401667 1102 8 0.15268664158593737 1102 9 0.17031808724083675 1102 10-11 0.1941853638188178 1102 12-13 0.20351861023867457 1102 14-15 0.1928876223064222 1102 16-17 0.21186408451338679 1102 18-19 0.24801749553338226 1102 20-21 0.25254900666880786 1102 22-23 0.2319598496149453 1102 24-25 0.14893285176785298 1102 26-27 -0.020198261764264203 1102 28-29 -0.0040425612807482025 1102 30-31 3.9942026437955747E-4 1102 32-33 -0.11878605757760141 1102 34-35 -0.12433172306761264 1102 36-37 0.018995819188162955 1102 38-39 -0.04732928886100751 1102 40-41 -0.11064455037923437 1102 42-43 -0.16513401835149466 1102 44-45 -0.17403665275979563 1102 46-47 -0.18042052074435588 1102 48-49 -0.3628697369417324 1102 50-51 -0.6760940028000917 1102 52-53 -0.7050785312883718 1102 54-55 -0.5227280679594841 1102 56-57 -0.44139335302003957 1102 58-59 -0.4669775918051897 1102 60-61 -0.6794062271170347 1102 62-63 -0.5363544647684009 1102 64-65 -0.44476648415437836 1102 66-67 -0.3781216036101611 1102 68-69 -0.43848503290909235 1102 70-71 -0.15150312259999765 1102 72-73 -0.12070524021132911 1102 74-75 -0.18157360245260534 1102 76-77 -0.21528348305155376 1102 78-79 -0.19008216608193607 1102 80-81 -0.2765298100400635 1102 82-83 -0.3276086730283403 1102 84-85 -0.44449573141920595 1102 86-87 -0.4270663807706079 1102 88-89 -0.45336054157676386 1102 90-91 -0.5956985093189324 1102 92-93 -0.5174406525382267 1102 94-95 -0.33033573678581263 1102 96-97 -0.41294138706270545 1102 98-99 -0.41425319062462407 1102 100-101 -0.13117979260032087 1103 1 0.16571895101413503 1103 2 0.13111628936333375 1103 3 0.12863358065146002 1103 4 0.11586854825992532 1103 5 0.12843309106325052 1103 6 0.09352453959201057 1103 7 0.11050967690970026 1103 8 0.07005477393778392 1103 9 0.09694804970141746 1103 10-11 0.017810479789204692 1103 12-13 0.1619690426304743 1103 14-15 0.21963756192280925 1103 16-17 0.19174392677108898 1103 18-19 0.23861345355604868 1103 20-21 0.11896014343825101 1103 22-23 0.1727731886367465 1103 24-25 0.13044643751597107 1103 26-27 0.16162243390008513 1103 28-29 0.2429512378303187 1103 30-31 0.29138126906381956 1103 32-33 0.27299804674721884 1103 34-35 0.25890443215551784 1103 36-37 0.30052960805094386 1103 38-39 0.4260076787756333 1103 40-41 0.25278408747936965 1103 42-43 0.24381709011100838 1103 44-45 0.3028375529417673 1103 46-47 0.28067663253520436 1103 48-49 0.21520514325040807 1103 50-51 0.2771532810660915 1103 52-53 0.26296668757117914 1103 54-55 0.3687913642765608 1103 56-57 0.423718689616436 1103 58-59 0.40665079729551223 1103 60-61 0.4116449217516944 1103 62-63 0.441551905646115 1103 64-65 0.4593940030679988 1103 66-67 0.41962941726789893 1103 68-69 0.6406773166115585 1103 70-71 0.5609992193811806 1103 72-73 0.4103347574410492 1103 74-75 0.4600220147130436 1103 76-77 0.40178566577655417 1103 78-79 0.36528847642225415 1103 80-81 0.4351198250623405 1103 82-83 0.5617730665162668 1103 84-85 0.5694128150626341 1103 86-87 0.4897165384553084 1103 88-89 0.21981909007192257 1103 90-91 0.18271621209253297 1103 92-93 0.5515874083465135 1103 94-95 0.4787617596081013 1103 96-97 0.43439642933048717 1103 98-99 0.47161162683628177 1103 100-101 0.7626043072098376 1104 1 0.14098368943676576 1104 2 0.11835656607729561 1104 3 0.10879998187049722 1104 4 0.07834316862928148 1104 5 0.0452284289112157 1104 6 0.12189298901309087 1104 7 0.11789155271262786 1104 8 0.10816981205282161 1104 9 0.12781089635373633 1104 10-11 0.14359517136337274 1104 12-13 0.1266888231397303 1104 14-15 0.2237560528834095 1104 16-17 0.2205804556076103 1104 18-19 0.24213620707880779 1104 20-21 0.23484323300554166 1104 22-23 0.2610345268980865 1104 24-25 0.22671983642094062 1104 26-27 0.14902886867493947 1104 28-29 0.21686847490545347 1104 30-31 0.2437212608774928 1104 32-33 0.2849036770739062 1104 34-35 0.489957347418958 1104 36-37 0.436072033067056 1104 38-39 0.3873749164586613 1104 40-41 0.2713248954938621 1104 42-43 0.27421489682460276 1104 44-45 0.4901473323568126 1104 46-47 0.579668944685416 1104 48-49 0.5066767768273053 1104 50-51 0.647124466826746 1104 52-53 0.5236869219756244 1104 54-55 0.5768506381253147 1104 56-57 0.6161226320203781 1104 58-59 0.5871683593920167 1104 60-61 0.647736276264105 1104 62-63 0.5643639889845176 1104 64-65 0.6037347000402704 1104 66-67 0.47369056343431026 1104 68-69 0.5540431115563038 1104 70-71 0.6174281179153382 1104 72-73 0.37125620520460245 1104 74-75 0.32826981717137116 1104 76-77 0.2689473618856155 1104 78-79 0.3774899491500463 1104 80-81 0.2872563745673098 1104 82-83 0.4172672246420035 1104 84-85 0.5901379936825464 1104 86-87 0.4931481076763511 1104 88-89 0.4590360398151887 1104 90-91 0.36245465498886986 1104 92-93 0.4034818128724922 1104 94-95 0.30813566792937763 1104 96-97 0.4894879581062277 1104 98-99 0.5595072147318696 1104 100-101 0.7245500586292692 1105 1 0.17053881344989463 1105 2 0.08161460078879657 1105 3 0.12329445941830386 1105 4 0.1423497634828621 1105 5 0.1267189469649992 1105 6 0.12470170321950036 1105 7 0.04633204319564044 1105 8 0.08420260927766776 1105 9 0.0259807138334196 1105 10-11 0.1169077759063697 1105 12-13 0.20002245154481102 1105 14-15 0.11299626157289566 1105 16-17 0.14541138871668835 1105 18-19 0.1534943485693958 1105 20-21 0.12280541218417795 1105 22-23 0.13327952497089512 1105 24-25 0.27755009455339774 1105 26-27 0.14219104195213106 1105 28-29 0.07753190684807265 1105 30-31 0.25226819849379467 1105 32-33 0.257564357472468 1105 34-35 0.18373188678430097 1105 36-37 0.22943098231655057 1105 38-39 0.223089109963027 1105 40-41 0.26456821350191007 1105 42-43 0.4225714393951563 1105 44-45 0.5132145662989132 1105 46-47 0.4986473140820493 1105 48-49 0.7773613063999534 1105 50-51 0.8772610666043335 1105 52-53 0.6934214127278864 1105 54-55 0.7682420120715747 1105 56-57 0.6159211533420788 1105 58-59 0.7535279670203607 1105 60-61 0.7738185902167558 1105 62-63 0.7566387339779723 1105 64-65 0.3982227597775463 1105 66-67 0.31622414167718915 1105 68-69 0.42546421398124323 1105 70-71 0.5901519621100775 1105 72-73 0.43057945814933163 1105 74-75 0.37714614706896654 1105 76-77 0.36973372809547556 1105 78-79 0.31885366011988836 1105 80-81 0.25123041931862744 1105 82-83 0.3334221262447947 1105 84-85 0.4311690112559141 1105 86-87 0.41018684137594263 1105 88-89 0.08895751384610762 1105 90-91 0.2098730538189102 1105 92-93 0.4355920738081309 1105 94-95 0.48958358499946186 1105 96-97 0.4894722824195874 1105 98-99 0.22811858864125867 1105 100-101 0.31071884704623187 1106 1 -0.1757408081905396 1106 2 -0.08577051070276553 1106 3 -0.14738757060074192 1106 4 -0.04038325309353752 1106 5 -0.05531456957905334 1106 6 -0.06121316412905742 1106 7 -0.008181743075624581 1106 8 -0.19651287433042341 1106 9 -0.13110425968176287 1106 10-11 -0.09040042555663774 1106 12-13 -0.22134456388091195 1106 14-15 -0.3150339288485 1106 16-17 -0.37170406551239665 1106 18-19 -0.2589933883206612 1106 20-21 -0.1453142617788572 1106 22-23 -0.11328942737041103 1106 24-25 -0.15962431004092537 1106 26-27 -0.16656783514046225 1106 28-29 -0.03540062462504778 1106 30-31 -0.1603270865262445 1106 32-33 -0.042306060146536595 1106 34-35 -0.06943378804961498 1106 36-37 -0.23678578924597105 1106 38-39 -0.4714112459076176 1106 40-41 -0.5277908853322018 1106 42-43 -0.5693388427306942 1106 44-45 -0.4941590384171164 1106 46-47 -0.4549990047115955 1106 48-49 -0.42518898328049914 1106 50-51 -0.4445592248423509 1106 52-53 -0.49772134298746096 1106 54-55 -0.6417756153292373 1106 56-57 -0.4956901974449508 1106 58-59 -0.4042704965278219 1106 60-61 -0.41252952996433834 1106 62-63 -0.2818101596752811 1106 64-65 -0.5976296422181164 1106 66-67 -0.39004005598751945 1106 68-69 -0.5141055410363577 1106 70-71 -0.5748093817693842 1106 72-73 -0.5470264855024496 1106 74-75 -0.5312171627553433 1106 76-77 -0.4624566513308288 1106 78-79 -0.3023084892338588 1106 80-81 -0.36884488185985376 1106 82-83 -0.4080103099214547 1106 84-85 -0.18024516795949097 1106 86-87 -0.29606074316300734 1106 88-89 -0.33834301060404215 1106 90-91 -0.5737331136728763 1106 92-93 -0.4408276926637811 1106 94-95 -0.5067899445203103 1106 96-97 -0.4944553927745652 1106 98-99 -0.35397604671812033 1106 100-101 -0.5883262479721054 1107 1 0.07840178442327783 1107 2 0.10195225444198996 1107 3 0.11442952171755394 1107 4 0.06055586008629632 1107 5 0.04242704004391129 1107 6 0.008226527810379025 1107 7 0.05055376132933276 1107 8 0.0210720854511095 1107 9 -0.10612018171048021 1107 10-11 -0.05571004966577675 1107 12-13 -0.013517883963629629 1107 14-15 0.05220745893026191 1107 16-17 0.15758632773305692 1107 18-19 0.05905875407221117 1107 20-21 0.16253632164684717 1107 22-23 0.16430848287354394 1107 24-25 0.13502981765293498 1107 26-27 0.0950147244668571 1107 28-29 0.2243912183845609 1107 30-31 0.23753575972671825 1107 32-33 0.26590430382531594 1107 34-35 0.1734176712923201 1107 36-37 0.16051507897530115 1107 38-39 0.14621202497643537 1107 40-41 0.22187757628056914 1107 42-43 0.3109938089706361 1107 44-45 0.33849593736012196 1107 46-47 0.2175184276586677 1107 48-49 0.19195908258185312 1107 50-51 0.007426239859860573 1107 52-53 0.07789314908754363 1107 54-55 0.25546917788086176 1107 56-57 0.018650270376184608 1107 58-59 0.1216189677552677 1107 60-61 0.022595775173762433 1107 62-63 0.036981599835776535 1107 64-65 0.08235436207795743 1107 66-67 0.18283863043282622 1107 68-69 0.25124837005240863 1107 70-71 0.39930524701404124 1107 72-73 0.1853389467499298 1107 74-75 0.08835481709366988 1107 76-77 -0.14927280224382855 1107 78-79 0.17026141624905122 1107 80-81 0.2574360433041569 1107 82-83 0.22576747586054324 1107 84-85 0.2769401284358253 1107 86-87 0.2529676447090736 1107 88-89 0.2480923214009536 1107 90-91 0.49796676959286756 1107 92-93 0.5855263346680744 1107 94-95 0.498795863344597 1107 96-97 0.727403137749068 1107 98-99 0.6744073229889338 1107 100-101 0.8630763640592178 1108 1 0.008792390946211981 1108 2 0.006541251175733009 1108 3 -0.017966906263161775 1108 4 -0.0320943562474838 1108 5 -0.007852207525715471 1108 6 0.04176174175591285 1108 7 -0.018504556816580475 1108 8 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Count 2 6.0 3 5.0 4 10.0 5 19.0 6 57.0 7 119.0 8 248.0 9 530.0 10 883.0 11 1574.0 12 2321.0 13 3669.0 14 4793.0 15 5973.0 16 7632.0 17 10230.0 18 10573.0 19 7723.0 20 6426.0 21 6191.0 22 6359.0 23 7167.0 24 8231.0 25 9215.0 26 10135.0 27 11362.0 28 13842.0 29 16920.0 30 20468.0 31 25033.0 32 31547.0 33 40785.0 34 58107.0 35 101632.0 36 150750.0 37 252898.0 38 247664.0 39 31650.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.75443944382935 17.722637209369204 13.882967633398996 19.639955713402447 2 13.479300364917579 22.800068309694584 40.54926387315969 23.171367452228154 3 23.319648705292785 28.291141060714452 30.589906927753074 17.799303306239686 4 11.80298934533481 16.49801573036939 39.224822781715964 32.47417214257984 5 12.2448777848773 41.039729463285134 33.79902601750077 12.916366734336792 6 26.193167929601564 37.44625936417099 18.778847398162394 17.58172530806505 7 24.464121385537837 38.00163917926007 19.48691172875897 18.047327706443124 8 21.364694759904992 32.60453634114493 21.364155553778172 24.666613345171903 9 24.7443942883795 21.58842650049562 21.68341647577306 31.983762735351817 10-11 22.55951910218989 25.567961234848376 27.58682976979597 24.285689893165774 12-13 31.193114009231167 23.833073317324974 25.30231354335917 19.67149913008469 14-15 26.254906753706127 25.60826671172013 29.24088206536587 18.895944469207873 16-17 18.95477729244299 29.362115518466215 31.470678235018962 20.212428954071832 18-19 17.621311846664845 32.76740909025551 31.62137655110932 17.98990251197032 20-21 20.038202582426273 30.052653241692667 28.037918601808848 21.87122557407221 22-23 18.80423490589098 29.225290091592736 26.524014335629143 25.446460666887138 24-25 20.635004086900217 29.148203718607373 29.709585185245103 20.507207009247313 26-27 23.819323535148612 25.98845502863322 30.735520022579387 19.456701413638775 28-29 19.587266153883483 26.820151138934044 33.92335751664119 19.66922519054128 30-31 18.80603225157088 32.555367233669315 31.066895408860194 17.571705105899607 32-33 18.185032992660062 31.371402766575525 28.92915958022822 21.514404660536197 34-35 19.918798388731364 27.666343532072347 29.381473646553673 23.033384432642617 36-37 22.329601929689282 27.878941474839085 31.08968310030035 18.701773495171285 38-39 24.697159406012677 24.426699408295725 32.483243434671195 18.392897751020403 40-41 19.830017252637667 26.888903169964323 35.16167550570361 18.119404071694394 42-43 18.10869739104332 30.778795452727547 32.005757337999604 19.10674981822953 44-45 18.61205910701407 32.23129652138552 28.662992511643687 20.493651859956728 46-47 18.40168267578161 28.97178750152213 26.27599243856333 26.350537384132934 48-49 20.625979553395545 27.931336002990783 28.728863214804377 22.713821228809294 50-51 24.252618732655616 28.938298920154715 28.838681035846264 17.97040131134341 52-53 19.39021453118035 28.552453736322196 33.023261247789264 19.03407048470818 54-55 17.10435029548363 28.07849368053259 33.65435424964412 21.162801774339655 56-57 20.102143154988713 32.24357269184868 27.436661538240326 20.21762261492228 58-59 18.16954721267632 29.508012567040993 29.149127568406975 23.17331265187571 60-61 19.139439875192316 26.574519749234334 30.458763498066055 23.8272768775073 62-63 25.016265978403098 25.952323608494975 28.455981206953574 20.57542920614836 64-65 22.79672379829468 26.849783240111 31.457907961522423 18.895585000071893 66-67 18.44948380232073 28.010329345622388 32.76466813809366 20.77551871396322 68-69 17.901860035632524 32.071966012055746 30.460684027598354 19.56548992471338 70-71 18.577320013803615 30.47435547183919 27.2682951816757 23.680029332681496 72-73 18.137150056796123 28.899431319826878 26.245425755244657 26.717992868132338 74-75 20.047863315455736 28.44443326096021 28.55258853724819 22.95511488633586 76-77 22.42889700490316 25.502762520310007 29.95883179720189 22.109508677584945 78-79 19.302144592865254 25.797302543603607 32.45925417343667 22.44129869009447 80-81 18.59354105856471 30.763143988957108 29.778153622729953 20.865161329748226 82-83 18.501075936541085 29.92941371006914 27.867587686918416 23.701922666471358 84-85 19.39469757121423 26.676696175530783 28.13897340427683 25.789632848978155 86-87 21.266925425230667 26.6467100846642 29.62784060017129 22.458523889933847 88-89 23.482466660346 23.87136750658166 30.64367920420687 22.002486628865476 90-91 19.395435548727637 25.78603578606724 32.915255562450376 21.903273102754746 92-93 18.056399935475145 28.744519768375543 30.757955856192254 22.44112443995706 94-95 18.240048096970394 30.024839317296216 28.18121198613887 23.553900599594517 96-97 17.932342516571527 27.485189871597626 26.404670582483785 28.17779702934706 98-99 19.764287100809526 26.600671128876876 27.870810387220153 25.76423138309345 100-101 22.441927761082432 26.97474369850605 28.421157634405507 22.16217090600601 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6196.0 1 3643.0 2 1599.0 3 2077.0 4 1458.0 5 1423.0 6 3621.5 7 6982.5 8 8841.0 9 8372.5 10 6060.5 11 3894.5 12 3411.0 13 3248.5 14 2802.5 15 2259.5 16 1899.0 17 1769.0 18 1859.0 19 1998.5 20 2129.5 21 2406.0 22 2793.0 23 3292.5 24 4025.0 25 4838.5 26 5833.5 27 7201.5 28 8564.5 29 10016.5 30 11530.0 31 13150.5 32 15106.5 33 17225.0 34 19099.5 35 20455.0 36 21952.0 37 23208.0 38 23724.5 39 24389.5 40 25269.5 41 25945.5 42 26083.0 43 26219.5 44 27560.5 45 28973.0 46 35003.0 47 50096.0 48 157128.0 49 159036.0 50 55211.5 51 45438.5 52 34732.0 53 22154.5 54 17130.0 55 14615.0 56 12386.0 57 9898.0 58 7716.0 59 5857.5 60 4111.0 61 2927.0 62 2204.0 63 1626.0 64 1146.0 65 770.0 66 602.5 67 516.0 68 356.0 69 204.5 70 149.5 71 138.0 72 109.0 73 69.0 74 46.0 75 24.5 76 13.0 77 9.0 78 9.0 79 6.5 80 2.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015457172847139345 3 0.006110975311659741 4 0.0 5 8.986728399499619E-5 6 0.0 7 0.0 8 4.493364199749809E-4 9 2.696018519849886E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1453549398248665E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0045382978417473074 26-27 0.02093907717083411 28-29 8.986728399499619E-5 30-31 0.0 32-33 4.0440277797748283E-4 34-35 0.01909679784893669 36-37 0.0030554876558298704 38-39 0.018243058650984228 40-41 0.015142637353156857 42-43 0.007638719139574676 44-45 0.0022017484578774065 46-47 4.4933641997498096E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.0440277797748283E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.4933641997498096E-5 84-85 0.003190288581822365 86-87 2.696018519849886E-4 88-89 4.4933641997498096E-5 90-91 4.4933641997498096E-5 92-93 2.2466820998749045E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1112752.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.68152011152244 #Duplication Level Percentage of deduplicated Percentage of total 1 70.89255167084572 26.713391115421164 2 15.678200891324654 11.81556884397878 3 6.072704305790251 6.864861882898928 4 2.9254324090940416 4.409389606327068 5 1.5334821631380793 2.889196948547424 6 0.8923192371577355 2.0174367168514546 7 0.5808610509952156 1.5321409162553392 8 0.342842819048855 1.0335070864864384 9 0.2335257965956048 0.7919646300878923 >10 0.7342063900808107 4.315718732359778 >50 0.04883732589174535 1.330461155120229 >100 0.046692469777106047 3.6578465858813463 >500 0.006432125935384986 1.6970548171191109 >1k 0.007385033481367947 6.214694633375994 >5k 0.0023822688649574025 7.0824771732908545 >10k+ 0.002144041978461662 17.634289155998236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 45609 4.098756955727781 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 36002 3.2354019583878526 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 29536 2.654320100076207 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 17807 1.6002667260988972 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15956 1.4339223834241592 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 15124 1.3591528031403224 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13184 1.1848102721900298 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12341 1.109052151782248 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10885 0.9782053862855334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9930 0.8923821300703122 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 9147 0.8220160467022302 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9054 0.8136583892906954 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 8041 0.7226228306037643 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7683 0.6904503429335557 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7354 0.660884006499202 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7171 0.6444382935281177 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 7071 0.635451565128618 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6746 0.6062446978302444 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6701 0.6022006700504695 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4824 0.43351977799186164 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4406 0.39595525328195325 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4138 0.3718708211712942 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3658 0.3287345248536961 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 3114 0.27984672236041813 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2993 0.2689727809970236 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2950 0.26510848778523877 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2681 0.2409341883905848 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2598 0.23347520381900008 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2580 0.23185759270709017 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2464 0.2214329877636706 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2269 0.20390886738464636 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2109 0.18953010194544695 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2100 0.188721296389492 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2082 0.18710368527758206 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2017 0.1812623118179073 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1865 0.1676024846506679 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1851 0.16634434267473797 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.16373819143888305 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1671 0.15016823155563863 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1638 0.14720261118380376 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1558 0.14001322846420408 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1520 0.13659827167239422 RNA PCR Primer, Index 31 (95% over 22bp) GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1464 0.1315657037686744 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1434 0.12886968524882453 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1397 0.12554459574100968 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1332 0.11970322228133491 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1314 0.11808561116942498 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1146 0.10298790745826562 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1125 0.10110069449437072 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.986728399499619E-5 0.0 9 0.0 0.0 0.0 8.986728399499619E-5 0.0 10-11 0.0 0.0 0.0 1.348009259924943E-4 0.0 12-13 0.0 0.0 0.0 1.7973456798999238E-4 0.0 14-15 0.0 0.0 0.0 2.2466820998749047E-4 0.0 16-17 0.0 0.0 0.0 7.189382719599694E-4 0.0 18-19 0.0 0.0 0.0 0.0012132083339324484 0.0 20-21 0.0 0.0 0.0 0.0022017484578774065 0.0 22-23 0.0 0.0 0.0 0.006919780867614707 0.0 24-25 0.0 0.0 0.0 0.013704760809236919 0.0 26-27 0.0 0.0 0.0 0.025072972234603936 0.0 28-29 0.0 0.0 0.0 0.04668605403540052 0.0 30-31 0.0 0.0 0.0 0.10002228708643077 0.0 32-33 0.0 0.0 0.0 0.19146224855133936 0.0 34-35 0.0 0.0 0.0 0.38687865759845863 0.0 36-37 0.0 0.0 0.0 0.6607492055732095 0.0 38-39 0.0 0.0 0.0 0.9157476239090112 0.0 40-41 0.0 0.0 0.0 1.1676456209469857 0.0 42-43 0.0 0.0 0.0 1.4189145469969948 0.0 44-45 0.0 0.0 0.0 1.6915718866378133 0.0 46-47 0.0 0.0 0.0 2.0515352926797705 0.0 48-49 0.0 0.0 0.0 2.47054150430644 0.0 50-51 0.0 0.0 0.0 2.806285677311746 0.0 52-53 0.0 0.0 0.0 3.0813694336204294 0.0 54-55 0.0 0.0 0.0 3.36602405567458 0.0 56-57 0.0 0.0 0.0 3.65876673328828 0.0 58-59 0.0 0.0 0.0 4.118437890922685 0.0 60-61 0.0 0.0 0.0 4.755012797101241 0.0 62-63 0.0 0.0 0.0 5.226501502580988 0.0 64-65 0.0 0.0 0.0 5.557976979596532 0.0 66-67 0.0 0.0 0.0 5.944541101701008 0.0 68-69 0.0 0.0 0.0 6.353796713014221 0.0 70-71 0.0 0.0 0.0 6.808749838238889 0.0 72-73 0.0 0.0 0.0 7.357659208880325 0.0 74-75 0.0 0.0 0.0 7.880102664385236 0.0 76-77 0.0 0.0 0.0 8.324316649172502 0.0 78-79 0.0 0.0 0.0 8.7931093361324 0.0 80-81 0.0 0.0 0.0 9.284773246869023 0.0 82-83 0.0 0.0 0.0 9.765203747106273 0.0 84-85 0.0 0.0 0.0 10.583355500596719 0.0 86-87 0.0 0.0 0.0 11.477579909988929 0.0 88-89 0.0 0.0 0.0 12.059380706572533 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 50 0.0016166089 37.9961 4 CCGTTCA 50 0.0016166089 37.9961 9 TATCACG 60 0.003951145 31.666264 2 CTTCGTA 85 6.410896E-4 27.938309 9 ACGAGAT 35 0.008397433 27.14007 92-93 AGTACTC 575 0.0 24.780066 5 ATAGCCC 380 0.0 23.747562 3 CATGGGG 3045 0.0 23.084658 4 GTATAGC 465 0.0 22.47283 1 GTACTAG 175 4.0495015E-6 21.714008 1 AGTACTT 6895 0.0 21.66384 12-13 GAGTACT 6875 0.0 21.381441 12-13 AGAGCCG 115 0.0037254198 20.650055 5 AAGTACT 610 0.0 20.243822 4 GTACTTT 7295 0.0 20.11788 14-15 ATGGGGA 2015 0.0 19.799456 5 ACTGATC 145 6.150117E-4 19.653156 8 TACTTTT 7645 0.0 19.538544 14-15 AAAGTAC 590 0.0 18.515049 3 ACTTTTT 8015 0.0 18.429176 16-17 >>END_MODULE