##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938180_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1090737 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12106493132625 33.0 31.0 34.0 31.0 34.0 2 32.186824138174465 34.0 31.0 34.0 30.0 34.0 3 32.21047420230541 34.0 31.0 34.0 30.0 34.0 4 35.65705023300759 37.0 35.0 37.0 35.0 37.0 5 35.682287297487846 37.0 35.0 37.0 35.0 37.0 6 35.752677318180275 37.0 36.0 37.0 35.0 37.0 7 35.73475274057816 37.0 36.0 37.0 35.0 37.0 8 35.757313632892256 37.0 36.0 37.0 35.0 37.0 9 37.38795053252984 39.0 38.0 39.0 35.0 39.0 10-11 37.31386576232401 39.0 37.5 39.0 35.0 39.0 12-13 37.20460752683736 39.0 37.0 39.0 34.5 39.0 14-15 38.258791991103266 40.0 38.0 41.0 34.0 41.0 16-17 38.14432489225175 40.0 38.0 41.0 33.5 41.0 18-19 38.507801147297656 40.0 38.0 41.0 34.0 41.0 20-21 38.52846515704519 40.0 38.0 41.0 34.5 41.0 22-23 38.45367719257713 40.0 38.0 41.0 34.0 41.0 24-25 38.333387883605305 40.0 38.0 41.0 34.0 41.0 26-27 37.99630020802448 40.0 37.5 41.0 33.5 41.0 28-29 37.80392156862745 40.0 37.5 41.0 33.5 41.0 30-31 37.682349640655815 40.0 37.0 41.0 33.0 41.0 32-33 37.47468638177673 40.0 36.5 41.0 33.0 41.0 34-35 37.29837623551782 40.0 36.0 41.0 33.0 41.0 36-37 36.955398047375304 39.0 35.0 41.0 32.0 41.0 38-39 36.53205493166547 39.0 35.0 40.5 30.5 41.0 40-41 36.41085614589035 39.0 35.0 41.0 30.5 41.0 42-43 36.41421259203639 39.0 35.0 41.0 30.5 41.0 44-45 36.198064244634594 39.0 35.0 41.0 30.0 41.0 46-47 35.79069656571657 39.0 35.0 41.0 29.0 41.0 48-49 35.42832690190211 39.0 35.0 40.5 27.0 41.0 50-51 34.69378411111019 38.5 34.5 39.5 25.0 40.5 52-53 34.56932560278051 38.0 34.5 40.0 25.0 40.5 54-55 34.743484451338865 38.0 35.0 40.0 24.5 41.0 56-57 34.61462020633755 38.0 35.0 40.0 23.5 41.0 58-59 34.34101116951199 38.0 34.0 40.0 22.5 41.0 60-61 33.849651657548975 38.0 34.0 40.0 20.5 41.0 62-63 33.278467219870606 37.0 33.0 40.0 18.5 41.0 64-65 32.59138545772262 36.0 32.0 39.0 13.0 41.0 66-67 32.140863929618234 36.0 31.0 39.0 8.0 40.5 68-69 31.63320534647674 35.5 31.0 39.0 4.5 40.0 70-71 31.009447740381045 35.0 30.5 37.5 2.0 40.0 72-73 30.177374564170833 35.0 29.5 37.0 2.0 39.0 74-75 29.537329347037826 34.0 29.0 36.5 2.0 39.0 76-77 28.68865180148835 34.0 27.5 36.0 2.0 38.5 78-79 27.987494693954638 34.0 26.0 35.0 2.0 37.0 80-81 27.433071858752385 33.0 26.0 35.0 2.0 37.0 82-83 26.76964703682006 33.0 25.0 35.0 2.0 36.0 84-85 26.10326595687136 32.0 23.5 35.0 2.0 36.0 86-87 25.25458474407671 32.0 19.5 35.0 2.0 35.0 88-89 24.250985342937845 31.0 11.5 34.0 2.0 35.0 90-91 23.318898597920487 30.5 4.5 34.0 2.0 35.0 92-93 22.580576710976157 30.0 2.0 34.0 2.0 35.0 94-95 21.624355367059152 29.5 2.0 34.0 2.0 35.0 96-97 20.26409391081443 28.5 2.0 34.0 2.0 35.0 98-99 18.701011334538023 26.0 2.0 34.0 2.0 35.0 100-101 14.702653801970595 10.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0777381348481967 1101 2 0.12598362405263686 1101 3 0.057177479440326806 1101 4 -0.15747550083963802 1101 5 0.009206597671230554 1101 6 0.07217542984002989 1101 7 -0.013524556370789753 1101 8 -0.05746903633699674 1101 9 -0.14020861497097314 1101 10-11 -0.030398900036438192 1101 12-13 0.14270698714231855 1101 14-15 -0.04675337321499029 1101 16-17 0.056078983288266215 1101 18-19 0.1958605432247822 1101 20-21 0.27430946881487017 1101 22-23 0.13785499679721624 1101 24-25 0.24354325870906024 1101 26-27 0.12173977786811463 1101 28-29 0.14925831330888428 1101 30-31 0.21958408893186032 1101 32-33 0.09680013904839768 1101 34-35 0.21613988588205046 1101 36-37 0.12523781721208138 1101 38-39 0.019042608680791773 1101 40-41 0.130526668194463 1101 42-43 0.26816045972189784 1101 44-45 0.30542860787119963 1101 46-47 0.2725951321011095 1101 48-49 0.33064474616571005 1101 50-51 0.41301779631068314 1101 52-53 0.3029747230326123 1101 54-55 0.3667197512970617 1101 56-57 0.3550710525013585 1101 58-59 0.3054117218425816 1101 60-61 0.41255967541700755 1101 62-63 0.14350417294479456 1101 64-65 0.0120247672939513 1101 66-67 0.06472685045725513 1101 68-69 0.049123163341800336 1101 70-71 -0.15857997940871726 1101 72-73 -0.23695537484827867 1101 74-75 -0.2193347738995115 1101 76-77 -0.26159273586916143 1101 78-79 -0.33384458242893444 1101 80-81 -0.32905420195521273 1101 82-83 -0.5786376516663339 1101 84-85 -0.6624250296768466 1101 86-87 -0.817197026160688 1101 88-89 -0.9389713590461106 1101 90-91 -1.3215178325337256 1101 92-93 -1.369053338242793 1101 94-95 -1.7684181161061119 1101 96-97 -1.935578224862116 1101 98-99 -1.8564297127257383 1101 100-101 -2.072054760834746 1102 1 0.04989312242520327 1102 2 0.1638398134643566 1102 3 0.09610325485933657 1102 4 -0.01942858709490025 1102 5 0.01947314294543645 1102 6 0.055872570455427706 1102 7 -0.022673402772319662 1102 8 0.12639859653469898 1102 9 0.12350663582041932 1102 10-11 0.09740305754035461 1102 12-13 0.02831001405838407 1102 14-15 0.06351329542553685 1102 16-17 0.12982675344200345 1102 18-19 0.24858237437576491 1102 20-21 0.28645858415497827 1102 22-23 0.34119291935770946 1102 24-25 0.12101212721783838 1102 26-27 0.054061630731567334 1102 28-29 -0.09209185706000511 1102 30-31 0.06756038779416684 1102 32-33 0.17974116747939917 1102 34-35 0.12110278962918386 1102 36-37 0.1427595358529743 1102 38-39 -0.12460575289109954 1102 40-41 0.15145761512967937 1102 42-43 0.2373762446894645 1102 44-45 0.22183928942077813 1102 46-47 0.13295185815631783 1102 48-49 -0.1784529032124027 1102 50-51 0.008786454849968095 1102 52-53 0.011263336074414099 1102 54-55 0.0797135558403923 1102 56-57 -0.08678146552863097 1102 58-59 -0.18154537381813185 1102 60-61 -0.045744975246577724 1102 62-63 -0.3925897285644666 1102 64-65 -0.3568467022703459 1102 66-67 -0.36394829394302164 1102 68-69 -0.47087081638171213 1102 70-71 -0.4036761681295822 1102 72-73 -0.6649106767626769 1102 74-75 -0.5416218868068334 1102 76-77 -0.5053541009915286 1102 78-79 -0.7378254902570234 1102 80-81 -0.8062289651535579 1102 82-83 -0.7551026884171606 1102 84-85 -0.5462694696801016 1102 86-87 -0.6391707886114375 1102 88-89 -0.9313941995803354 1102 90-91 -0.9622454964443854 1102 92-93 -0.6799870485836053 1102 94-95 -0.8788402263117128 1102 96-97 -0.7507649891362149 1102 98-99 -1.0141942656338507 1102 100-101 -0.9878905553261923 1103 1 0.24029051907725574 1103 2 0.2612723167047548 1103 3 0.2596398368620427 1103 4 0.25503985006899654 1103 5 0.30599998997116273 1103 6 0.2666362008020826 1103 7 0.2818066170837241 1103 8 0.26867266509320586 1103 9 0.2043028080164433 1103 10-11 0.31004542813134606 1103 12-13 0.22962478750644522 1103 14-15 0.3427768292497575 1103 16-17 0.22871398766058348 1103 18-19 0.23439012926393588 1103 20-21 0.21372892970529023 1103 22-23 0.1916727737467241 1103 24-25 0.29428368619304024 1103 26-27 0.35807627456378555 1103 28-29 0.20768614134048136 1103 30-31 0.30092060951999855 1103 32-33 0.4632585828843787 1103 34-35 0.21588782037847665 1103 36-37 0.23638974461721318 1103 38-39 0.1595942140154989 1103 40-41 0.061402183674388766 1103 42-43 0.3343330302166123 1103 44-45 0.3011255749617163 1103 46-47 0.22339337934988635 1103 48-49 0.2852753447675198 1103 50-51 0.16111953998276363 1103 52-53 0.2051320931412235 1103 54-55 0.09786322076966059 1103 56-57 0.039207889425725284 1103 58-59 0.1446452993569025 1103 60-61 0.20619926236408048 1103 62-63 0.23508876899489906 1103 64-65 -0.08383299608463801 1103 66-67 -0.04871636432167037 1103 68-69 0.040291313730531186 1103 70-71 -0.03757002102939211 1103 72-73 -0.08237710074436322 1103 74-75 -0.17716696278377952 1103 76-77 -0.17238962124745072 1103 78-79 0.009644243065277891 1103 80-81 0.0053937148607836605 1103 82-83 -0.008329295515697055 1103 84-85 -0.18537553404678064 1103 86-87 -0.02838371710210197 1103 88-89 -0.11951297733053323 1103 90-91 -0.2683146892410555 1103 92-93 -0.4328241187140449 1103 94-95 -1.1719409405777306 1103 96-97 -1.1831579181784342 1103 98-99 -1.5003812158006973 1103 100-101 -1.7865792291079412 1104 1 -0.008775729472723981 1104 2 0.028809634894102487 1104 3 0.06798814846095524 1104 4 0.07572770301429443 1104 5 0.082482838263644 1104 6 0.14148163784874868 1104 7 0.042903281073428445 1104 8 0.19506883065721325 1104 9 0.15672610017548294 1104 10-11 0.1754115351845158 1104 12-13 0.149272766396642 1104 14-15 0.3156327064662179 1104 16-17 0.19539850077134702 1104 18-19 0.18877812370604374 1104 20-21 0.16826192650567862 1104 22-23 0.402005864878312 1104 24-25 0.4454366939562391 1104 26-27 0.2630391794549851 1104 28-29 0.2925746648021814 1104 30-31 0.2504040187007135 1104 32-33 0.26628323647215524 1104 34-35 0.2901768307931505 1104 36-37 0.04825157489753451 1104 38-39 0.08793596067247478 1104 40-41 0.06265462162613034 1104 42-43 0.1417444035004891 1104 44-45 0.2695819850220147 1104 46-47 0.016986505919419415 1104 48-49 -0.043220327469789765 1104 50-51 0.028201773847662537 1104 52-53 0.19048685495493345 1104 54-55 0.16029231302302804 1104 56-57 0.037181043274820524 1104 58-59 0.027741529932960418 1104 60-61 0.04147339649285442 1104 62-63 0.2049410259327047 1104 64-65 0.10492820574589956 1104 66-67 0.008256815022349429 1104 68-69 -0.21462283212590805 1104 70-71 -0.14821638099217083 1104 72-73 0.0451644241068081 1104 74-75 -0.007625743871646051 1104 76-77 -0.23332407380777553 1104 78-79 -0.42135694244850797 1104 80-81 -0.4129301216148029 1104 82-83 -0.4956838151276628 1104 84-85 -0.5472074077959341 1104 86-87 -0.4019448475468579 1104 88-89 -0.3937219974332784 1104 90-91 -0.4648980512561742 1104 92-93 -0.5737170145545534 1104 94-95 -0.5829174597562847 1104 96-97 -1.2865366324696126 1104 98-99 -1.1979939352163562 1104 100-101 -1.7616993739190878 1105 1 0.03942124822503246 1105 2 0.0482465529191316 1105 3 0.14685829103490278 1105 4 0.0348628524990815 1105 5 -0.06543799438437503 1105 6 0.02020577269525603 1105 7 0.05171279869967549 1105 8 0.21748361379534487 1105 9 0.28726739641246013 1105 10-11 0.17984370651563353 1105 12-13 0.16769830678394015 1105 14-15 0.1829878601638839 1105 16-17 0.18145418569475424 1105 18-19 0.23043216618679452 1105 20-21 0.2486599562670193 1105 22-23 0.2995941404000959 1105 24-25 0.31740580726227563 1105 26-27 0.4174668782173825 1105 28-29 0.39445545208969435 1105 30-31 0.3524406245115941 1105 32-33 0.40727735865118575 1105 34-35 0.1782486342771321 1105 36-37 0.011550541589883778 1105 38-39 0.016858187030621252 1105 40-41 0.1393736029192496 1105 42-43 0.24710369504560248 1105 44-45 0.1957672878726271 1105 46-47 0.21783802529429863 1105 48-49 0.2819022353409437 1105 50-51 0.4407067874529247 1105 52-53 0.5428646744766965 1105 54-55 0.7320395292096862 1105 56-57 0.6216072704178259 1105 58-59 0.75564054798771 1105 60-61 0.7118724938996266 1105 62-63 0.7318788745405769 1105 64-65 0.650806661526623 1105 66-67 0.7616595741644261 1105 68-69 0.46953157825968006 1105 70-71 0.37568877746661045 1105 72-73 0.1849841363042266 1105 74-75 0.17658637707815217 1105 76-77 0.21298057448961671 1105 78-79 0.0908618267582888 1105 80-81 0.07571821040561844 1105 82-83 -0.12435096079201458 1105 84-85 0.0297554006309646 1105 86-87 0.12082197003293516 1105 88-89 0.14138766706635408 1105 90-91 0.24901481156292604 1105 92-93 0.43709505964969253 1105 94-95 0.023456094954774187 1105 96-97 0.1405850242852651 1105 98-99 0.2785063230384175 1105 100-101 0.01757791668534381 1106 1 0.13015769242985442 1106 2 0.18195991092781583 1106 3 0.10846812563733721 1106 4 0.10902420346459252 1106 5 0.14426414461742354 1106 6 0.13777918792975896 1106 7 0.14554779691263064 1106 8 0.04149806361774466 1106 9 0.2362851275318789 1106 10-11 0.25118667575625153 1106 12-13 0.2861218303517248 1106 14-15 0.2495517465118624 1106 16-17 -0.21489267062078454 1106 18-19 0.1557099658398826 1106 20-21 0.3343255345527112 1106 22-23 0.3559264378531921 1106 24-25 0.32422989541508684 1106 26-27 0.245279555533628 1106 28-29 0.24745385081675408 1106 30-31 0.24759258390023575 1106 32-33 0.4510831785614897 1106 34-35 0.3201152581354947 1106 36-37 0.35795757184445165 1106 38-39 0.21453462489497355 1106 40-41 0.21159040679461327 1106 42-43 -0.07673154547851624 1106 44-45 0.2595269273136154 1106 46-47 0.5180004747370859 1106 48-49 0.5105253425373846 1106 50-51 0.3226881254547891 1106 52-53 0.28413339553816996 1106 54-55 0.31127679424358234 1106 56-57 0.16816079796356576 1106 58-59 0.3414075940732815 1106 60-61 0.6510347232431073 1106 62-63 0.5302467069258725 1106 64-65 0.18430466548841196 1106 66-67 0.10918476947731648 1106 68-69 0.021810211867030915 1106 70-71 0.21256490194185673 1106 72-73 0.2695421506450053 1106 74-75 0.3767040999186051 1106 76-77 0.0027928035302089427 1106 78-79 -0.05724632882970582 1106 80-81 0.0779399008894508 1106 82-83 -0.06721146427422653 1106 84-85 -0.07845521219416085 1106 86-87 -0.07039939938860584 1106 88-89 -0.357237217078616 1106 90-91 -0.41585017063729524 1106 92-93 -0.44800351051251397 1106 94-95 -0.9036839669557288 1106 96-97 -1.1371792917611856 1106 98-99 -1.571429868355093 1106 100-101 -1.7921679392889391 1107 1 -0.0898913262608545 1107 2 0.007013700521639521 1107 3 0.09394774171995834 1107 4 0.06291857005704315 1107 5 0.018559267070600072 1107 6 0.12485284693180176 1107 7 0.07736130137482888 1107 8 0.11075473820474002 1107 9 0.09878957102006325 1107 10-11 0.08430609800924316 1107 12-13 0.022010247347374445 1107 14-15 -0.01529885873799941 1107 16-17 0.046256275103175426 1107 18-19 0.04789503946146567 1107 20-21 0.13392421740925897 1107 22-23 0.200574607136069 1107 24-25 0.09477097195533446 1107 26-27 0.1082695085729739 1107 28-29 -0.00642274182295921 1107 30-31 -0.05943654667283482 1107 32-33 0.010277924955246931 1107 34-35 -0.06510616789679347 1107 36-37 -0.17404209138592108 1107 38-39 -0.1581935833034933 1107 40-41 -0.024087529181734624 1107 42-43 -0.10026425890627166 1107 44-45 -0.2050149724412904 1107 46-47 -0.08505477419179641 1107 48-49 -0.12124793666334455 1107 50-51 -0.2510868750129447 1107 52-53 -0.2708910202600592 1107 54-55 -0.2874649969966683 1107 56-57 -0.4643674064930252 1107 58-59 -0.5309997475046728 1107 60-61 -0.6682303705846522 1107 62-63 -0.47151852722257104 1107 64-65 -0.481973468807837 1107 66-67 -0.3974570760202596 1107 68-69 -0.37124484996761353 1107 70-71 -0.4646629227134227 1107 72-73 -0.44637769818284667 1107 74-75 -0.36924156791427976 1107 76-77 -0.5239420278043347 1107 78-79 -0.8011152506056085 1107 80-81 -0.7217841351074803 1107 82-83 -0.6800723222884066 1107 84-85 -0.4706346729885631 1107 86-87 -0.3916916592999229 1107 88-89 -0.7109033286990432 1107 90-91 -1.1167753516509755 1107 92-93 -1.0542992316672581 1107 94-95 -1.3287284018375765 1107 96-97 -1.2651190099972602 1107 98-99 -1.3771119204047082 1107 100-101 -1.4347122338501084 1108 1 0.03569263059279848 1108 2 0.06531063546029969 1108 3 -0.1279862474174891 1108 4 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2316 84-85 -0.5051814589732508 2316 86-87 -0.5241805818383725 2316 88-89 -0.4610106900911397 2316 90-91 -0.2108903338401511 2316 92-93 -0.3918217279127134 2316 94-95 -0.38007010894576965 2316 96-97 -0.3742459694978173 2316 98-99 -0.4855326529445172 2316 100-101 -0.44922029526330753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5916.0 3 1078.0 4 783.0 5 730.0 6 815.0 7 912.0 8 1183.0 9 1329.0 10 1761.0 11 2597.0 12 3746.0 13 5200.0 14 6251.0 15 8269.0 16 9612.0 17 12630.0 18 12524.0 19 9878.0 20 9776.0 21 10535.0 22 11246.0 23 12862.0 24 14468.0 25 16628.0 26 18987.0 27 22111.0 28 25539.0 29 30298.0 30 35968.0 31 43539.0 32 52990.0 33 67219.0 34 88937.0 35 128667.0 36 143296.0 37 165323.0 38 99938.0 39 7196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.744791853171996 17.345155819746562 13.626434771111484 19.28361755596996 2 13.444758375224747 22.914174586284076 40.82697317726488 22.814093861226286 3 23.459235405010556 28.59568364613722 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18.858266975136402 29.22883944003281 26.640973103515115 25.271920481315675 24-25 20.64367131728244 29.159034704353054 29.743129625979865 20.45416435238464 26-27 23.95711864313404 26.0007822987463 30.76008770560592 19.28201135251373 28-29 19.560296110248192 27.01464394431776 33.91539156394241 19.50966838149164 30-31 18.792371617497924 32.80507335159666 30.92450085580687 17.478054175098546 32-33 18.35605661420055 31.43156669463472 28.85812300681698 21.35425368434774 34-35 20.141390587022844 27.778519588153 29.201648379129036 22.87844144569512 36-37 22.568647720093214 27.891206090212556 31.009264981782493 18.530881207911733 38-39 24.892585165928857 24.51080844568625 32.37704166154513 18.219564726839764 40-41 19.799203989866626 27.082967337164003 35.14228325649516 17.97554541647421 42-43 17.9853013084973 31.017385775974766 31.746219595992397 19.251093319535535 44-45 18.697067029626872 32.4134132628447 28.38463753813192 20.504882169396513 46-47 18.50156033000842 29.11648262539054 26.232073633633412 26.14988341096762 48-49 20.808134636824622 28.158548738525006 28.634860855499856 22.398455769150512 50-51 24.59239105507743 29.185662732608165 28.493456791708937 17.728489420605463 52-53 19.477708712007967 28.872890044808173 32.81799957080994 18.831401672373914 54-55 17.243487489577323 28.35729440610693 33.43438781622333 20.96483028809242 56-57 20.1119644398958 32.39801895664622 27.459349956966733 20.03066664649124 58-59 18.42722746302391 29.418601237978876 28.99416968456122 23.160001614435995 60-61 19.41568054121182 26.262641085798876 30.681861956499805 23.6398164164895 62-63 25.53818762048078 25.396419894813942 28.71147309554943 20.35391938915585 64-65 23.1467413859915 26.457061228420553 31.69308517590302 18.703112209684928 66-67 18.716135869712268 27.558746742279265 33.13911975159005 20.58599763641842 68-69 18.083230086607983 31.818767708858452 30.86241481285088 19.235587391682678 70-71 18.8968920192014 30.18307925025387 27.720710117793402 23.199318612751334 72-73 18.598433806444746 28.631144631195998 26.695784743326435 26.074636819032822 74-75 20.50733364580018 28.28184203791978 28.993972936457634 22.21685137982241 76-77 23.137118068912773 25.374589234517003 30.15333869175865 21.33495400481158 78-79 19.96791356561992 25.883637844202433 32.65746103593607 21.490987554241574 80-81 19.179459667657092 30.9409504420234 29.9762022669748 19.903387623344702 82-83 19.166207391743324 29.792423018339516 28.181583090857572 22.859786499059595 84-85 20.32338228618851 26.52848909450209 28.49083681413657 24.657291805172832 86-87 22.504852347857746 26.44134104084409 29.920679645082792 21.13312696621537 88-89 24.726576494133372 23.735953832103313 30.769026115539923 20.768443558223392 90-91 20.69874486588192 25.77673756912416 32.9640439641128 20.560473600881117 92-93 19.284582087943093 28.937998490621435 30.936250547665857 20.841168873769618 94-95 19.56944406213585 30.004679457182963 28.268921981520734 22.15695449916045 96-97 19.693163614878316 27.240857938166652 26.386699719806746 26.679278727148287 98-99 21.653122733854726 26.380098239189355 27.746172464864284 24.220606562091636 100-101 24.749767508471137 25.87766125915392 28.616241633288563 20.756329599086378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6455.0 1 3825.5 2 1638.5 3 2064.0 4 1464.5 5 1389.5 6 3238.0 7 6416.5 8 8662.0 9 8304.0 10 5946.0 11 3951.0 12 3545.5 13 3311.0 14 2809.5 15 2294.0 16 1941.0 17 1866.5 18 1989.5 19 2122.5 20 2307.0 21 2631.0 22 3062.5 23 3549.5 24 4183.0 25 5050.0 26 6216.5 27 7511.5 28 8784.5 29 10307.5 30 11754.0 31 13329.0 32 15066.0 33 16941.0 34 18803.0 35 20205.5 36 21622.0 37 22460.0 38 22787.5 39 23594.5 40 24588.0 41 25151.5 42 25136.5 43 25200.0 44 26530.5 45 28543.0 46 36067.0 47 56251.5 48 143741.0 49 152364.0 50 65789.0 51 37283.5 52 26660.5 53 21050.5 54 17277.0 55 14473.5 56 12169.5 57 9844.0 58 7670.0 59 6015.5 60 4527.0 61 3403.0 62 2558.5 63 1862.0 64 1446.0 65 1130.5 66 874.5 67 713.5 68 538.5 69 372.0 70 304.0 71 268.5 72 224.0 73 154.0 74 96.5 75 79.5 76 58.5 77 36.5 78 31.0 79 21.5 80 16.5 81 15.0 82 8.0 83 5.0 84 4.0 85 3.0 86 2.0 87 1.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14907351634720378 2 0.05656725681809639 3 0.15732481799003792 4 0.13229586967344098 5 0.19867300733357354 6 0.2027069770256258 7 0.1683265535138168 8 0.07334490349185917 9 0.12111077189093246 10-11 0.1052499365108179 12-13 0.15067793611108818 14-15 0.13710912896509425 16-17 0.13522966581311535 18-19 0.1345420573428792 20-21 0.13610063654208118 22-23 0.19596841401731124 24-25 0.2187970152291524 26-27 0.1502653710289465 28-29 0.12954543579249625 30-31 0.1028662271473325 32-33 0.12826189998138873 34-35 0.09722783769139581 36-37 0.09062679637712849 38-39 0.05234992486731449 40-41 0.061930602885938595 42-43 0.06491023959029536 44-45 0.04061474030861702 46-47 0.05345009841969237 48-49 0.04451118830662203 50-51 0.05491699648952956 52-53 0.028512831232460255 54-55 0.051891519220490366 56-57 0.027641860503494424 58-59 0.05253328712604414 60-61 0.04061474030861702 62-63 0.05257912769072654 64-65 0.039835450709016014 66-67 0.04056889974393461 68-69 0.06513944241370744 70-71 0.055742126653812965 72-73 0.052991692772868255 74-75 0.0447403911300341 76-77 0.02223267387096981 78-79 0.022461876694381874 80-81 0.02722929542135272 82-83 0.02292028234120599 84-85 0.02127002201263916 86-87 0.026174962433657245 88-89 0.06559784806053155 90-91 0.11111752879016665 92-93 0.0810919589231868 94-95 0.07948753915930237 96-97 0.07105287525773857 98-99 0.058905125616899395 100-101 0.13293763757899474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1090737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.85804673531674 #Duplication Level Percentage of deduplicated Percentage of total 1 73.43660815153063 29.27039759786851 2 14.328630092215317 11.422224157371678 3 5.635862417475522 6.739034028886637 4 2.665968381213234 4.250411693330953 5 1.384992954744763 2.76015569591506 6 0.7963300609702042 1.9044096472132823 7 0.4871082065839508 1.3590627164225604 8 0.30593653463976334 0.975522615657003 9 0.20888381259671737 0.7493130688257995 >10 0.6467392586948253 4.119598725481067 >50 0.04398597529318787 1.28441725980152 >100 0.04237329822484953 3.4452986880284113 >500 0.005757241603157097 1.6063068341960183 >1k 0.006448110595535949 5.614822591691693 >5k 0.0023028966412628384 6.986792206019 >10k+ 0.002072606977136555 17.512232473290855 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 45886 4.206880302034312 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35326 3.2387275759417715 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 27996 2.566704897697612 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16500 1.5127386345195954 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15090 1.3834682421151938 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14286 1.3097566141058752 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12797 1.1732434124816524 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12004 1.100540276895347 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10903 0.9995993534646758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9476 0.8687703818610719 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 9139 0.8378738412651262 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8767 0.8037684611414118 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7567 0.693751105903623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7391 0.6776152271354139 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7330 0.6720226782441597 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6879 0.630674488900624 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6690 0.6133467554506724 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6571 0.6024367010562584 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6308 0.5783245640333096 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4512 0.41366525569408574 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4152 0.38066004912274914 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3932 0.3604902006624878 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3213 0.29457146864917944 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3064 0.2809109803738206 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2916 0.26734217322782666 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.24433020975725586 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2569 0.23552882133823275 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 0.23287006858665288 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2533 0.23222830068109912 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2115 0.19390558860660267 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.18987161891455043 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2040 0.1870295039042409 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1976 0.18116191162489215 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1951 0.17886988339077156 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1770 0.16227559897573843 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1766 0.16190887445827915 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1765 0.1618171933289143 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1665 0.15264908039243189 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1557 0.14274751842103092 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1473 0.1350463035543857 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1470 0.13477126016629123 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1463 0.13412949226073748 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1384 0.12688668304091638 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.12001059833855457 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1194 0.1094672684615998 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.168112936482397E-5 0.0 9 0.0 0.0 0.0 9.168112936482397E-5 0.0 10-11 0.0 0.0 0.0 1.3752169404723596E-4 0.0 12-13 0.0 0.0 0.0 2.750433880944719E-4 0.0 14-15 0.0 0.0 0.0 3.6672451745929587E-4 0.0 16-17 0.0 0.0 0.0 5.959273408713558E-4 0.0 18-19 0.0 0.0 0.0 0.0012835358111075354 0.0 20-21 0.0 0.0 0.0 0.001971144281343715 0.0 22-23 0.0 0.0 0.0 0.005271664938477378 0.0 24-25 0.0 0.0 0.0 0.01132261947655576 0.0 26-27 0.0 0.0 0.0 0.021361703142003984 0.0 28-29 0.0 0.0 0.0 0.0447403911300341 0.0 30-31 0.0 0.0 0.0 0.09773208390290235 0.0 32-33 0.0 0.0 0.0 0.192163647148671 0.0 34-35 0.0 0.0 0.0 0.38538162728503755 0.0 36-37 0.0 0.0 0.0 0.6529071627715939 0.0 38-39 0.0 0.0 0.0 0.9084224703113584 0.0 40-41 0.0 0.0 0.0 1.1493146377174333 0.0 42-43 0.0 0.0 0.0 1.4049674669512449 0.0 44-45 0.0 0.0 0.0 1.6822112021504725 0.0 46-47 0.0 0.0 0.0 2.041280345307806 0.0 48-49 0.0 0.0 0.0 2.4573751509300594 0.0 50-51 0.0 0.0 0.0 2.7918279108529367 0.0 52-53 0.0 0.0 0.0 3.0615996340089318 0.0 54-55 0.0 0.0 0.0 3.348790771744243 0.0 56-57 0.0 0.0 0.0 3.6364861556910606 0.0 58-59 0.0 0.0 0.0 4.080772908592997 0.0 60-61 0.0 0.0 0.0 4.7175900331610645 0.0 62-63 0.0 0.0 0.0 5.1884643135788 0.0 64-65 0.0 0.0 0.0 5.526859362064366 0.0 66-67 0.0 0.0 0.0 5.91673336468828 0.0 68-69 0.0 0.0 0.0 6.333836662733546 0.0 70-71 0.0 0.0 0.0 6.8022355526584315 0.0 72-73 0.0 0.0 0.0 7.346408896003345 0.0 74-75 0.0 0.0 0.0 7.85757703277692 0.0 76-77 0.0 0.0 0.0 8.29452929532967 0.0 78-79 0.0 0.0 0.0 8.764165880500983 0.0 80-81 0.0 0.0 0.0 9.249479938793678 0.0 82-83 0.0 0.0 0.0 9.731355954735193 0.0 84-85 0.0 4.584056468241198E-5 0.0 10.517521639038558 0.0 86-87 0.0 9.168112936482397E-5 0.0 11.366168013004051 0.0 88-89 0.0 9.168112936482397E-5 0.0 11.924826974788607 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCG 25 0.0016152587 38.00419 94-95 TACCACG 185 0.0 25.678505 94-95 ACCACTC 960 0.0 24.989733 94-95 TCGCCGG 60 1.7102227E-4 23.752617 94-95 CGGCGGT 50 0.0016530583 23.752617 94-95 GTAGCCC 590 0.0 23.380093 3 GAGTACT 6600 0.0 22.182352 12-13 CATGGGG 3055 0.0 22.109423 4 AACGACG 65 2.9468845E-4 21.918447 92-93 TGACGCG 55 0.0028763446 21.593288 94-95 CGCCGTC 55 0.0028763446 21.593288 94-95 CGCCGGA 55 0.0028763446 21.593288 94-95 GTGTAGC 610 0.0 21.076265 1 GTACTTT 6990 0.0 20.910707 14-15 GTCTAAT 230 8.788629E-8 20.702934 1 ACAACGA 115 0.0037029942 20.671535 8 TGTAGCC 700 0.0 20.382786 2 GTAAGCG 70 4.85005E-4 20.359388 94-95 AGTACTT 6610 0.0 20.243132 12-13 GGAACGC 260 1.4739271E-8 20.114994 8 >>END_MODULE