##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938180_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1090737 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49246060232668 33.0 31.0 34.0 31.0 34.0 2 32.67974314614797 34.0 31.0 34.0 31.0 34.0 3 32.74114841616265 34.0 31.0 34.0 31.0 34.0 4 36.12219077559485 37.0 35.0 37.0 35.0 37.0 5 36.12437828734149 37.0 35.0 37.0 35.0 37.0 6 36.19824852370461 37.0 36.0 37.0 35.0 37.0 7 36.1830661286818 37.0 36.0 37.0 35.0 37.0 8 36.21433397785167 37.0 37.0 37.0 35.0 37.0 9 37.854667990542175 39.0 38.0 39.0 35.0 39.0 10-11 37.8507385373376 39.0 38.0 39.0 35.0 39.0 12-13 37.784633234226035 39.0 38.0 39.0 35.0 39.0 14-15 38.86348771518707 40.0 38.0 41.0 35.0 41.0 16-17 38.785107225664845 40.0 38.0 41.0 35.0 41.0 18-19 39.19260967584303 40.5 38.5 41.0 36.0 41.0 20-21 39.214248714401364 41.0 39.0 41.0 36.0 41.0 22-23 39.15610912621466 40.5 39.0 41.0 35.5 41.0 24-25 39.04538490946947 40.0 38.5 41.0 35.0 41.0 26-27 38.655672265633235 40.0 38.0 41.0 34.5 41.0 28-29 38.475007724135146 40.0 38.0 41.0 34.5 41.0 30-31 38.35416924519843 40.0 38.0 41.0 34.0 41.0 32-33 38.19230712811613 40.0 37.0 41.0 34.0 41.0 34-35 38.07915152782018 40.0 37.0 41.0 34.0 41.0 36-37 37.97240306324989 40.0 37.0 41.0 34.0 41.0 38-39 37.69925426569375 40.0 36.5 41.0 33.0 41.0 40-41 37.4718969834158 40.0 36.0 41.0 33.0 41.0 42-43 37.42794871724348 40.0 36.5 41.0 33.0 41.0 44-45 37.24642099791242 40.0 36.0 41.0 33.0 41.0 46-47 36.851648930952194 40.0 36.0 41.0 31.5 41.0 48-49 36.514581883625475 40.0 36.0 41.0 31.0 41.0 50-51 36.203888746783136 39.0 35.0 41.0 30.0 41.0 52-53 35.44460717844906 39.0 35.0 40.0 28.0 41.0 54-55 35.28967615474674 38.0 35.0 40.0 27.5 41.0 56-57 35.183316876570615 38.0 35.0 40.0 27.0 41.0 58-59 34.90533144103482 38.0 34.5 40.0 26.5 41.0 60-61 34.44150743946524 38.0 34.0 40.0 25.0 41.0 62-63 33.911099559288814 37.5 33.5 40.0 23.5 41.0 64-65 33.20723510800495 36.5 32.0 39.0 22.0 41.0 66-67 32.68726466600106 36.0 32.0 39.0 20.0 40.0 68-69 32.26138335822476 35.5 32.0 39.0 18.5 40.0 70-71 31.699521516185847 35.0 31.0 37.5 15.0 39.5 72-73 31.087899282778526 35.0 30.5 37.0 9.5 39.0 74-75 30.533878469328535 34.5 30.0 36.5 7.0 39.0 76-77 28.901572514730866 32.5 28.5 35.0 4.0 37.0 78-79 29.519280541505424 34.0 29.0 35.0 2.0 37.0 80-81 29.395071864253254 34.0 29.0 35.0 2.0 37.0 82-83 28.93779389532032 34.0 29.0 35.0 2.0 36.5 84-85 28.48120124282939 34.0 29.0 35.0 2.0 36.0 86-87 27.85342846167316 33.0 27.0 35.0 2.0 35.5 88-89 26.9959009367061 33.0 25.5 35.0 2.0 35.0 90-91 26.441148049438134 32.0 25.0 35.0 2.0 35.0 92-93 25.92194131124185 32.0 24.0 34.0 2.0 35.0 94-95 25.279382197541665 32.0 20.5 34.0 2.0 35.0 96-97 24.245616954407893 31.0 9.5 34.0 2.0 35.0 98-99 22.94668971530259 31.0 2.0 34.0 2.0 35.0 100-101 18.707834702591 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11503957510664264 1101 2 0.056618913792114256 1101 3 0.0711677095574359 1101 4 -0.016315168428704396 1101 5 0.10606653222572504 1101 6 0.1093328543354275 1101 7 0.025918854371397515 1101 8 -0.009507493394863786 1101 9 -0.05150392321845487 1101 10-11 0.04630974093749529 1101 12-13 0.1057702684076105 1101 14-15 0.1658972163181076 1101 16-17 0.08544451857343915 1101 18-19 0.06158635954377445 1101 20-21 0.08472810676664722 1101 22-23 0.21438991770581595 1101 24-25 0.21960045393335292 1101 26-27 0.2164026368646077 1101 28-29 0.2388983889234524 1101 30-31 0.3596397594859937 1101 32-33 0.21455648721116916 1101 34-35 0.14467692069920446 1101 36-37 0.22027303740389925 1101 38-39 0.33396482242834225 1101 40-41 0.23526878960494457 1101 42-43 0.423406288215034 1101 44-45 0.3274703376060657 1101 46-47 0.401974200063151 1101 48-49 0.4043987994891296 1101 50-51 0.15487940634582031 1101 52-53 0.2683324002163303 1101 54-55 0.14408039946255968 1101 56-57 0.12274816794845833 1101 58-59 0.15923919399905628 1101 60-61 0.14960908478430213 1101 62-63 0.25335794710635184 1101 64-65 0.4041070802385889 1101 66-67 0.029140667348784177 1101 68-69 0.2000366104183442 1101 70-71 -0.34220051781589333 1101 72-73 -0.11301743138641385 1101 74-75 -0.020960411362231923 1101 76-77 -0.05313988013851301 1101 78-79 -0.24515809356465113 1101 80-81 -0.3666706666723485 1101 82-83 -0.4034019983349175 1101 84-85 -0.41602293204941887 1101 86-87 -0.5967236939238312 1101 88-89 -0.1978896049883474 1101 90-91 -0.4479419431868976 1101 92-93 -0.37674034865590755 1101 94-95 -0.32979521276293866 1101 96-97 -0.189441407180027 1101 98-99 -0.43747648943841355 1101 100-101 -0.9993225326785726 1102 1 0.11587106411347037 1102 2 0.08638350617744806 1102 3 0.19507560919646494 1102 4 0.08472925084900851 1102 5 0.0715560374527513 1102 6 0.09572184808568807 1102 7 0.01162069771097407 1102 8 0.1323131961241799 1102 9 0.2412924705372319 1102 10-11 0.22349462017420052 1102 12-13 0.30286523826921297 1102 14-15 0.22853646605349098 1102 16-17 0.42661099648963585 1102 18-19 0.4159314447528857 1102 20-21 0.2568037697989354 1102 22-23 0.21638290062944776 1102 24-25 0.3568991509382613 1102 26-27 0.32423775530860866 1102 28-29 0.21555007891868883 1102 30-31 0.21537148214863322 1102 32-33 0.2544266318211754 1102 34-35 0.20385327779042228 1102 36-37 0.2432062197368552 1102 38-39 0.3763220320795355 1102 40-41 0.2849169488560648 1102 42-43 0.13809823017364664 1102 44-45 0.11221707888469012 1102 46-47 0.113514749339366 1102 48-49 0.12325188747152538 1102 50-51 0.2284174537530319 1102 52-53 0.2512029864777574 1102 54-55 0.2633515759084659 1102 56-57 0.19177470897886195 1102 58-59 0.17842820847486252 1102 60-61 0.3260377284702898 1102 62-63 0.043127548164335394 1102 64-65 -0.15941300503220646 1102 66-67 -0.3073452923301616 1102 68-69 -0.3467081224565618 1102 70-71 -0.16246997453528422 1102 72-73 -0.3588926784274733 1102 74-75 -0.46436055989528313 1102 76-77 -0.31535129547097185 1102 78-79 -0.14671978782836348 1102 80-81 -0.08123307399340263 1102 82-83 -0.07691549584415114 1102 84-85 -0.28272562168195847 1102 86-87 -0.3426199132395418 1102 88-89 -0.32468427655019383 1102 90-91 -0.5080676041219654 1102 92-93 -0.28714555767546557 1102 94-95 -0.7651167177333669 1102 96-97 -1.1125946750305147 1102 98-99 -1.3796910876219677 1102 100-101 -1.639439533297871 1103 1 0.1643046428895687 1103 2 0.2016175041366921 1103 3 0.1689249238871824 1103 4 0.18107242947066737 1103 5 0.16501043749688193 1103 6 0.17014753959998785 1103 7 0.060633411094499934 1103 8 0.1292037948390501 1103 9 0.15814528761342928 1103 10-11 0.2508794137540846 1103 12-13 0.14474425429194326 1103 14-15 0.20214447465777852 1103 16-17 0.2831510808778219 1103 18-19 0.1981527017507716 1103 20-21 0.22879847485396 1103 22-23 0.3000264846453362 1103 24-25 0.25720181771524864 1103 26-27 0.36572516602535643 1103 28-29 0.3206285378690765 1103 30-31 0.45236163686030295 1103 32-33 0.43280698536905504 1103 34-35 0.3619328950100211 1103 36-37 0.44151845602377904 1103 38-39 0.3472998043417448 1103 40-41 0.4139157354311678 1103 42-43 0.4847585539374748 1103 44-45 0.3816697966806615 1103 46-47 0.3933884428392602 1103 48-49 0.12656467531520832 1103 50-51 -0.11567468380523138 1103 52-53 0.07289502475580178 1103 54-55 0.1623718731975643 1103 56-57 0.2001687135567849 1103 58-59 0.13369695451054753 1103 60-61 0.07900924056317393 1103 62-63 0.03773263351774858 1103 64-65 -0.0625217168713732 1103 66-67 0.07772061870372937 1103 68-69 -0.17463964668369414 1103 70-71 -0.527793600469483 1103 72-73 -0.2874109211676057 1103 74-75 -0.21905223966142273 1103 76-77 0.030836757809414195 1103 78-79 -0.2842987293909225 1103 80-81 -0.005321626432660764 1103 82-83 -0.018412558804357104 1103 84-85 -0.0695385887815938 1103 86-87 -0.1761428442122117 1103 88-89 -0.47993716532877073 1103 90-91 -0.38149222010056505 1103 92-93 -0.1802779864556996 1103 94-95 -0.16947677399215877 1103 96-97 -0.5694008855597303 1103 98-99 -0.569316304797443 1103 100-101 -1.4031284828580048 1104 1 0.07789279794967285 1104 2 0.09202758992709903 1104 3 0.04202714576947386 1104 4 0.07298034181913238 1104 5 0.03629614631939404 1104 6 0.05482445522454782 1104 7 0.03947581424566948 1104 8 0.006060143711536625 1104 9 0.2185746220685445 1104 10-11 0.03407061290764801 1104 12-13 0.1331807145772288 1104 14-15 0.04369354600530784 1104 16-17 0.20883657554301038 1104 18-19 0.2312530752513453 1104 20-21 0.20683141429077523 1104 22-23 0.1821284133360379 1104 24-25 0.16728920155401283 1104 26-27 0.09546148481383909 1104 28-29 0.10783200007923455 1104 30-31 0.007583633877395357 1104 32-33 0.16128608590827298 1104 34-35 0.09045820509199132 1104 36-37 0.16943985518499005 1104 38-39 0.22900815520283402 1104 40-41 0.2025676277916162 1104 42-43 0.21371249736424858 1104 44-45 0.0793398796666267 1104 46-47 0.16693937531596958 1104 48-49 0.2749148352958315 1104 50-51 0.36487428556262813 1104 52-53 -0.08851490806508622 1104 54-55 0.23885822704156823 1104 56-57 0.395595243570682 1104 58-59 0.4561089014301203 1104 60-61 0.2596152548148325 1104 62-63 0.33991914160600345 1104 64-65 0.2538622748682684 1104 66-67 0.3931644852633198 1104 68-69 0.1790242920265186 1104 70-71 -0.07542767188075672 1104 72-73 -0.1258496122584063 1104 74-75 -0.04907282680943226 1104 76-77 -0.13990750264669316 1104 78-79 -0.3977893663815024 1104 80-81 -0.26083012261479865 1104 82-83 -0.15052882838764248 1104 84-85 -0.40306472744847 1104 86-87 -0.38410968806857326 1104 88-89 -0.5157208791737844 1104 90-91 -0.4524445828422863 1104 92-93 -0.22820684288105397 1104 94-95 -0.2719982215937229 1104 96-97 -0.5354352782069967 1104 98-99 -0.6900251910518556 1104 100-101 -1.2640042971020158 1105 1 0.09288600253073298 1105 2 0.10819809001799285 1105 3 -0.04828700168678779 1105 4 -0.03243088923376547 1105 5 0.0414295010056378 1105 6 0.09322795017631336 1105 7 0.1461913040496583 1105 8 0.07397970672714393 1105 9 0.1520458836503309 1105 10-11 0.06058284610450215 1105 12-13 0.11221158107249352 1105 14-15 0.19340340614375862 1105 16-17 0.06629581024321851 1105 18-19 0.08689298441491644 1105 20-21 0.07417646863970617 1105 22-23 0.20971117749893864 1105 24-25 0.18774326827319499 1105 26-27 0.10085597980312144 1105 28-29 0.2396587991339345 1105 30-31 0.28429857543103054 1105 32-33 0.1752477141541391 1105 34-35 0.3161223815478138 1105 36-37 0.2467878816279736 1105 38-39 0.18519862314894908 1105 40-41 0.12928242506883691 1105 42-43 0.2450955021801846 1105 44-45 0.3224817004926379 1105 46-47 0.36301534550269565 1105 48-49 0.351956995748516 1105 50-51 0.31336760700758504 1105 52-53 0.3733571649272349 1105 54-55 0.5195753962229688 1105 56-57 0.6246470301454679 1105 58-59 0.6356464666791268 1105 60-61 0.4826882269377464 1105 62-63 0.4925145299548177 1105 64-65 0.42713680113666896 1105 66-67 0.3357136185949159 1105 68-69 0.39127653336056056 1105 70-71 0.30013248128308234 1105 72-73 0.3322135634962322 1105 74-75 0.35338044927501855 1105 76-77 0.3436276733849546 1105 78-79 0.4921753436084835 1105 80-81 0.6989917825840593 1105 82-83 0.790736244890347 1105 84-85 0.3623418290487308 1105 86-87 0.3851519341176761 1105 88-89 0.29133971980416007 1105 90-91 0.20284156056269254 1105 92-93 0.27692795456929176 1105 94-95 0.17916854173813945 1105 96-97 0.18475505123375768 1105 98-99 -0.26874384339476265 1105 100-101 -1.2781707978508123 1106 1 0.08541373201455116 1106 2 0.11685465634764114 1106 3 0.203668146516776 1106 4 0.10880724004327647 1106 5 0.08849238681044369 1106 6 0.033549739401948386 1106 7 0.014686006521834827 1106 8 0.08557126702930873 1106 9 0.16141020050448418 1106 10-11 0.10791681752389337 1106 12-13 0.05849725381915505 1106 14-15 0.2055431954542044 1106 16-17 0.14965539395263505 1106 18-19 0.2238694660971987 1106 20-21 0.2752543494670334 1106 22-23 0.2080402400980077 1106 24-25 0.28965581009710917 1106 26-27 0.34207573381690537 1106 28-29 0.33088491381577256 1106 30-31 0.3320616395364624 1106 32-33 0.3824015474157818 1106 34-35 0.4670691114576968 1106 36-37 0.5064197503922472 1106 38-39 0.4679934199205533 1106 40-41 0.35243434811080476 1106 42-43 0.5311832067059541 1106 44-45 0.42695819731937235 1106 46-47 0.6149230411033315 1106 48-49 0.5487252715632422 1106 50-51 0.6567972388359635 1106 52-53 0.5106107900032129 1106 54-55 0.5402576636900775 1106 56-57 0.6296820542049488 1106 58-59 0.6687613161130272 1106 60-61 0.4543983985546234 1106 62-63 0.6846637492080561 1106 64-65 0.8152481067698005 1106 66-67 0.3368474329159099 1106 68-69 0.423460504073212 1106 70-71 0.055671310637464444 1106 72-73 0.06850026606533532 1106 74-75 0.019024360904136728 1106 76-77 0.022887453164493365 1106 78-79 0.011247251465622554 1106 80-81 0.1970260332670719 1106 82-83 0.379564488679609 1106 84-85 0.32790991747615195 1106 86-87 0.08338316358432607 1106 88-89 0.22482126679939896 1106 90-91 -0.15814452471871832 1106 92-93 -0.22394285117590584 1106 94-95 -0.17538216288253494 1106 96-97 -0.2702220200723602 1106 98-99 -0.5937295571323915 1106 100-101 -1.2183810229780754 1107 1 -0.03976353977724756 1107 2 -0.031219208458061587 1107 3 0.0070620360189153075 1107 4 0.030648621547143762 1107 5 -0.17823477366028584 1107 6 0.04816367043900982 1107 7 -0.052261925151348976 1107 8 -0.15561940071003022 1107 9 -0.05076027976642905 1107 10-11 -0.03710460476650468 1107 12-13 0.013026805354265036 1107 14-15 -0.04748586028117785 1107 16-17 -0.1702916583776215 1107 18-19 -0.0680892234947379 1107 20-21 -0.15589216403181183 1107 22-23 -0.14623205685797558 1107 24-25 -0.05504100368799669 1107 26-27 -0.09278366486221756 1107 28-29 -0.12975232916352297 1107 30-31 -0.18113785905097757 1107 32-33 -0.1855978538826477 1107 34-35 -0.11130279447132096 1107 36-37 -0.08113006344898821 1107 38-39 -0.1633467361160399 1107 40-41 -0.14476859301063882 1107 42-43 0.03120752895707568 1107 44-45 -0.2875313160485078 1107 46-47 -0.3380517573026296 1107 48-49 -0.18283097831982786 1107 50-51 -0.20285869485783792 1107 52-53 -0.14188317620756408 1107 54-55 -0.30105835718964613 1107 56-57 -0.03707504134408168 1107 58-59 0.030997279367717567 1107 60-61 0.06020299733102519 1107 62-63 0.1455096022642408 1107 64-65 -0.08570139649498998 1107 66-67 -0.3091740080839216 1107 68-69 -0.13397555647048165 1107 70-71 -0.2513868195427058 1107 72-73 -0.4134909779242619 1107 74-75 -0.5637975815082541 1107 76-77 -0.48524833983443827 1107 78-79 -0.16560077188373512 1107 80-81 0.032907901964335906 1107 82-83 -0.1279277001358956 1107 84-85 -0.6109121371927841 1107 86-87 -0.5412365673741562 1107 88-89 -0.716958747793953 1107 90-91 -0.6550406312876014 1107 92-93 -0.8374164428240185 1107 94-95 -1.1474667668975727 1107 96-97 -0.9522431991402556 1107 98-99 -1.0236178618714895 1107 100-101 -1.4492964750913053 1108 1 -0.10570223855046379 1108 2 -0.02401042726621938 1108 3 -0.08564942282873744 1108 4 0.034180848369274486 1108 5 -0.008904377519144191 1108 6 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1.350622667384087 2316 98-99 1.8975068883426154 2316 100-101 2.3726133165158885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 6.0 5 29.0 6 71.0 7 126.0 8 271.0 9 522.0 10 959.0 11 1570.0 12 2555.0 13 3929.0 14 4835.0 15 6356.0 16 8304.0 17 11377.0 18 11541.0 19 9086.0 20 8034.0 21 8079.0 22 8966.0 23 9881.0 24 11217.0 25 12672.0 26 13794.0 27 15824.0 28 19025.0 29 23138.0 30 28096.0 31 34792.0 32 43477.0 33 56953.0 34 79750.0 35 126489.0 36 163449.0 37 218245.0 38 139080.0 39 8236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.75712073409233 17.72158514211211 13.877099315731034 19.644194808064526 2 13.475447836739484 22.90688590249556 40.50593447866081 23.111731782104137 3 23.397550216526824 28.18562166533563 30.638064159423887 17.778763958713657 4 11.799636392640938 16.506545574231 39.11740410383071 32.576413929297345 5 12.22127381796078 41.14966155414629 33.735391543758666 12.893673084134255 6 26.108306638379492 37.43561580536857 18.779556218931013 17.67652133732092 7 24.4640091974509 37.999169368967955 19.4352992517903 18.101522181790845 8 21.454851169438648 32.6305057956226 21.24930207740271 24.66534095753605 9 24.666624493347157 21.611717581781857 21.680020023158654 32.04163790171233 10-11 22.557270909485972 25.580731193679135 27.546420447825643 24.315577449009247 12-13 31.208172908765768 23.845321302197483 25.265981220756277 19.680524568280468 14-15 26.252616350229246 25.599892549716387 29.238716574206247 18.90877452584812 16-17 18.99459523885927 29.394474216217954 31.392356319994864 20.218574224927913 18-19 17.67360051048053 32.72204940329337 31.635628020320205 17.968722065905894 20-21 20.04108231406838 30.07828651636462 28.022933117699317 21.857698051867683 22-23 18.80127840166786 29.206719860057923 26.523259044114212 25.468742694160003 24-25 20.631786636203064 29.170060892059734 29.666209926556377 20.531942545180826 26-27 23.842867478695922 26.05479592744372 30.641694459243716 19.460642134616638 28-29 19.60292886607296 26.82492543512604 33.88394744104722 19.688198257753783 30-31 18.819006684236413 32.54701909460938 31.108081457742067 17.525892763412138 32-33 18.2238706489282 31.37804988736973 28.931630631398768 21.466448832303296 34-35 19.874037147030318 27.713569751310928 29.38158272352263 23.03081037813612 36-37 22.30381382018748 27.915190782691052 31.096636611885337 18.68435878523613 38-39 24.727093528312917 24.392633255784634 32.509941895779136 18.370331320123316 40-41 19.823272376544622 26.8744533417437 35.120397756340324 18.18187652537136 42-43 18.08799246727123 30.778705921938705 32.0212944447959 19.112007165994164 44-45 18.588440659847425 32.27336195618192 28.612580301209185 20.52561708276147 46-47 18.451227841776415 29.02918674105076 26.214708430553706 26.304876986619114 48-49 20.663546432205955 27.876422212646577 28.71975649335766 22.74027486178981 50-51 24.199461348919833 28.965308578304967 28.827780642943097 18.0074494298321 52-53 19.40622428419815 28.536855400117823 33.01832168505406 19.03859863062997 54-55 17.143865713414677 28.184045789103312 33.56408205179927 21.108006445682744 56-57 20.13125300589465 32.239695827531676 27.352827500961656 20.276223665612015 58-59 18.178233492725564 29.51953536760549 29.07836017813904 23.223870961529908 60-61 19.167751982334224 26.620100092005426 30.333782129125748 23.878365796534602 62-63 25.03100552345347 25.97843345194522 28.400921935771684 20.58963908882962 64-65 22.772824244502754 26.877550094060332 31.319132508377855 19.03049315305906 66-67 18.43788539388688 28.069282645870867 32.64385364086906 20.84897831937319 68-69 17.965549892587013 32.06487423395167 30.387993589611316 19.581582283849993 70-71 18.55548535761673 30.480520683277597 27.170591077852162 23.793402881253513 72-73 18.15391639959236 28.88826338515591 26.16211131348296 26.795708901768766 74-75 20.046626993223725 28.475338584409986 28.396205653924095 23.081828768442193 76-77 22.40150205638672 25.574894200393395 29.846997491987658 22.17660625123223 78-79 19.222157876613245 25.86028627223878 32.34980840751037 22.567747443637604 80-81 18.608674921947706 30.769382412675416 29.711126291129318 20.910816374247556 82-83 18.479288136448158 29.913860108081835 27.797620548846613 23.809231206623394 84-85 19.383409582969968 26.595533962954505 28.107688512668993 25.913367941406534 86-87 21.275133286837125 26.62421480331049 29.585849012047287 22.514802897805094 88-89 23.44865309985108 23.9025258045821 30.454954773205785 22.193866322361036 90-91 19.366970550393006 25.83959304463615 32.699674167926865 22.09376223704398 92-93 18.045253092878514 28.802445508391187 30.583664759571167 22.568636639159127 94-95 18.262675944303005 29.93289347544127 27.971963529481343 23.83246705077438 96-97 17.89353419364363 27.609000935744476 26.23857770507406 28.25888716553783 98-99 19.75840170473092 26.581130594565256 27.675103519858574 25.98536418084525 100-101 22.188429085224264 26.706766090008227 28.242311157160245 22.86249366760726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5707.0 1 3370.5 2 1525.5 3 2006.0 4 1449.5 5 1408.5 6 3411.0 7 6678.0 8 8541.0 9 8078.5 10 5905.0 11 3874.0 12 3478.5 13 3241.0 14 2793.0 15 2307.5 16 1884.5 17 1755.0 18 1832.5 19 1975.5 20 2126.0 21 2332.0 22 2686.0 23 3189.5 24 3796.0 25 4629.0 26 5763.0 27 7057.0 28 8424.5 29 9886.0 30 11390.5 31 12999.5 32 14783.5 33 16687.0 34 18595.5 35 20207.5 36 21575.0 37 22621.5 38 23155.0 39 23904.5 40 24889.0 41 25533.5 42 25642.5 43 25830.5 44 26996.0 45 28388.0 46 34601.0 47 51328.0 48 147244.5 49 151551.5 50 59677.5 51 45950.0 52 34643.0 53 22271.5 54 17160.5 55 14410.5 56 12128.5 57 9794.5 58 7707.5 59 5800.0 60 4091.5 61 2933.5 62 2165.0 63 1562.0 64 1112.0 65 800.0 66 620.0 67 509.0 68 371.0 69 242.0 70 176.0 71 140.0 72 95.5 73 68.5 74 53.5 75 28.5 76 12.5 77 12.0 78 9.0 79 4.5 80 1.5 81 1.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007701214866645213 2 0.014760661827736658 3 0.010084924230130635 4 0.0 5 0.0014668980698371835 6 0.0017419414579316552 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010543329876954756 14-15 0.0 16-17 0.002933796139674367 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003346361221816075 26-27 0.019986486201531626 28-29 0.006967765831726621 30-31 0.003346361221816075 32-33 0.0 34-35 0.00898475067775275 36-37 5.959273408713559E-4 38-39 0.0022461876694381874 40-41 0.0017419414579316552 42-43 0.002750433880944719 44-45 0.0 46-47 1.8336225872964793E-4 48-49 6.417679055537677E-4 50-51 0.004538215903558786 52-53 0.008205461078151746 54-55 0.007747055431327626 56-57 0.01572331368606731 58-59 0.022049311612240163 60-61 0.00371308573927537 62-63 0.018840472084471326 64-65 0.01957392111938992 66-67 0.012101909076156764 68-69 0.029292120832061258 70-71 0.015585791992020075 72-73 0.00554670832657185 74-75 0.015310748603925603 76-77 0.02127002201263916 78-79 0.024891426622549707 80-81 0.011185097782508525 82-83 0.032959366006654216 84-85 0.022003471047557753 86-87 0.029842207608250203 88-89 0.021361703142003984 90-91 0.027320976550717545 92-93 0.022736920082476346 94-95 0.014439777874959776 96-97 0.014806502392419072 98-99 0.011460141170602995 100-101 0.010497489312272344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1090737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.995278507308825 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37250548893586 27.118182238165456 2 15.496727961258742 11.776049896800517 3 5.944370776553808 6.7757406961760855 4 2.864837334721806 4.354011696435653 5 1.5417255401014947 2.92891456389937 6 0.8490560966763945 1.9356073716928879 7 0.5284186651241868 1.4054190044897683 8 0.34378276349575726 1.0449697476026862 9 0.23265343539809868 0.7955758866269647 >10 0.7124628106986779 4.25044383756752 >50 0.04803941651386443 1.3140079392439132 >100 0.0470732234426098 3.68975390352026 >500 0.006276429790121886 1.6677636277105938 >1k 0.00748343551899148 6.2891281100401475 >5k 0.002414011457739187 7.077745591811895 >10k+ 0.0021726103119652685 17.57668588821627 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 44478 4.07779327188864 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35117 3.219566219904523 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 28664 2.627947892113314 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 17526 1.6068034732479048 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15747 1.443702744107883 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14813 1.3580725692811375 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13036 1.1951552023998453 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12044 1.1042075220699399 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10207 0.9357892874267582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9547 0.8752797420459745 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9341 0.8563934293968206 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8821 0.8087192421271122 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7796 0.7147460845281677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7494 0.6870583834599908 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7177 0.6579954654513417 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7001 0.6418595866831326 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6890 0.6316829813236371 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6575 0.6028034255737176 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 6524 0.5981276879761115 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4746 0.4351186399654545 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4456 0.40853111244965556 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4041 0.37048344376325365 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3728 0.34178725027206375 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2998 0.27486002583574226 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2991 0.2742182579301885 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.2625747545008559 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2633 0.24139641361758152 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2621 0.24029624006520361 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2557 0.23442864778585487 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2334 0.21398375593749916 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2123 0.1946390376415213 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2109 0.19335550183041375 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2080 0.19069674907883385 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.18684614164551125 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2036 0.18666277938678158 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1979 0.18143695501298662 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1852 0.16979345158365397 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.164567627209859 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1648 0.1510905011932299 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1626 0.14907351634720378 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1620 0.14852342957101483 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1549 0.14201406938611233 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1529 0.14018044679881583 RNA PCR Primer, Index 31 (95% over 22bp) TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1461 0.1339461300020078 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1395 0.12789517546392945 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1291 0.11836033800998774 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1187 0.10882550055604605 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1108 0.10158269133622497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.168112936482397E-5 0.0 9 0.0 0.0 0.0 9.168112936482397E-5 0.0 10-11 0.0 0.0 0.0 1.3752169404723596E-4 0.0 12-13 0.0 0.0 0.0 2.750433880944719E-4 0.0 14-15 0.0 0.0 0.0 3.6672451745929587E-4 0.0 16-17 0.0 0.0 0.0 5.959273408713558E-4 0.0 18-19 0.0 0.0 0.0 0.0013293763757899475 0.0 20-21 0.0 0.0 0.0 0.002062825410708539 0.0 22-23 0.0 0.0 0.0 0.00531750550315979 0.0 24-25 0.0 0.0 0.0 0.011368460041238171 0.0 26-27 0.0 0.0 0.0 0.02131586257732157 0.0 28-29 0.0 0.0 0.0 0.04460286943598686 0.0 30-31 0.0 0.0 0.0 0.0979612867263144 0.0 32-33 0.0 0.0 0.0 0.1928512556189072 0.0 34-35 0.0 0.0 0.0 0.3874444526957461 0.0 36-37 0.0 0.0 0.0 0.6571244947223758 0.0 38-39 0.0 0.0 0.0 0.9151151927549904 0.0 40-41 0.0 0.0 0.0 1.1572450554074905 0.0 42-43 0.0 0.0 0.0 1.4141814204524097 0.0 44-45 0.0 0.0 0.0 1.6930295754155218 0.0 46-47 0.0 0.0 0.0 2.053198892125233 0.0 48-49 0.0 0.0 0.0 2.4707605958173238 0.0 50-51 0.0 0.0 0.0 2.806680253810039 0.0 52-53 0.0 0.0 0.0 3.0785148023767412 0.0 54-55 0.0 0.0 0.0 3.3684563739929976 0.0 56-57 0.0 0.0 0.0 3.6592689163382186 0.0 58-59 0.0 0.0 0.0 4.108598131355222 0.0 60-61 0.0 0.0 0.0 4.750274355779624 0.0 62-63 0.0 0.0 0.0 5.223440664431481 0.0 64-65 0.0 0.0 0.0 5.562752524210694 0.0 66-67 0.0 0.0 0.0 5.95514775789214 0.0 68-69 0.0 0.0 0.0 6.374451403042164 0.0 70-71 0.0 0.0 0.0 6.846059132494817 0.0 72-73 0.0 0.0 0.0 7.394358126661148 0.0 74-75 0.0 0.0 0.0 7.910752087808518 0.0 76-77 0.0 0.0 0.0 8.35380114546403 0.0 78-79 0.0 0.0 0.0 8.826509048469063 0.0 80-81 0.0 0.0 0.0 9.318928394287532 0.0 82-83 0.0 0.0 0.0 9.810797653329812 0.0 84-85 0.0 0.0 0.0 10.613832665436306 0.0 86-87 0.0 0.0 0.0 11.48054022188667 0.0 88-89 0.0 0.0 0.0 12.052722150252535 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGACG 25 0.0016160136 38.00058 92-93 GTGTAGC 685 0.0 29.123863 1 TAGCCCT 875 0.0 28.761974 4 GCCCGCG 35 0.008397265 27.140158 94-95 ACCACTC 1150 0.0 26.845158 94-95 ATAGCCC 425 0.0 26.818403 3 GTATAGC 430 0.0 25.40678 1 GTAGCCC 625 0.0 24.315351 3 CAACGGC 50 0.0016539738 23.75036 28-29 CATGGGG 3035 0.0 23.624853 4 TATAGCC 530 0.0 22.402407 2 GTACCCT 260 6.2755134E-10 21.915873 6 CGCAGCG 110 5.6510544E-8 21.588762 94-95 GAGTACT 6625 0.0 21.50631 12-13 TCGTATC 100 5.395832E-7 21.372875 94-95 AGTACTT 6680 0.0 21.151493 12-13 TACCACG 80 4.995747E-5 20.779182 94-95 ATGGGGA 2255 0.0 20.425657 5 ATGGGGG 930 0.0 20.423395 5 GTACTTT 7135 0.0 19.968153 14-15 >>END_MODULE