FastQCFastQC Report
Thu 26 May 2016
SRR938179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738068
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109040.39823700507072873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89530.3269823831986642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54040.19736544161795835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.050.4760441
GTATCAA94700.041.917021
ATCAACG119150.033.028053
TATCAAC120450.032.9083372
TCAACGC121400.032.4159164
CAACGCA123250.031.8907875
AACGCAG126900.031.0421856
ACGCAGA144450.026.8685847
CGCAGAG145350.026.7022178
GCAGAGT155700.024.1631229
GTACATG89500.024.1486151
TGGTATC26200.023.582442
TACATGG91050.023.1765982
GAGTACT92500.022.85234612-13
ACATGGG93750.021.34313
AGAGTAC137000.021.34106310-11
GTACTTT100350.021.04237214-15
CAGAGTA148850.020.9185910-11
AGTACTT98150.020.18172512-13
GTGGTAT31350.020.0887741
Produced by FastQC (version 0.11.5)