FastQCFastQC Report
Thu 26 May 2016
SRR938179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738068
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105280.3845046945510484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88860.32453540233478495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55680.2033550664190955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60250.050.1278881
GTATCAA101050.040.2432021
ATCAACG125850.032.1558273
TATCAAC126850.031.9087492
TCAACGC127150.031.827064
CAACGCA129550.031.2007355
AACGCAG134450.030.1675976
CCGTCGT1153.8711496E-628.9091939
ACGCAGA152550.026.4014247
CGCAGAG154350.026.0319948
GGACCGA8050.025.9597476
AGGACCG9900.025.9383225
GCAGAGT168000.023.6337559
TAGCGCG1252.2398423E-422.8257264
GAGTACT101450.022.7520212-13
TGGTATC29700.022.5804882
GTACATG88950.022.2973291
ACCGAGT10250.021.7779858
CGCGGAA4800.021.76992282-83
TACATGG87750.021.6270082
Produced by FastQC (version 0.11.5)