FastQCFastQC Report
Thu 26 May 2016
SRR938178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718058
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108670.39980750962635825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87790.3229879568427164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52770.19414596745176152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.051.053681
GTATCAA94500.039.8663521
ATCAACG117700.031.415513
TATCAAC118950.031.165292
TCAACGC118850.031.151524
CAACGCA121950.030.203755
AACGCAG125900.029.4427156
ACGCAGA143900.025.6196967
CGCAGAG145500.025.4028038
GTACATG91550.024.356781
TACATGG89900.023.5784992
GCAGAGT155700.023.5228989
GGACCGA7400.022.4773626
GAGTACT94400.022.16822812-13
ACATGGG93500.021.8573653
CAGAGTA152050.020.65133310-11
GTACTTT101100.020.51077814-15
AGAGTAC138250.020.47925810-11
TGGTATC27650.020.2827972
ACCGAGT7150.019.933358
Produced by FastQC (version 0.11.5)