##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938178_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718058 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.833995080311016 33.0 31.0 34.0 30.0 34.0 2 32.03293932653387 34.0 31.0 34.0 30.0 34.0 3 32.11512925772739 34.0 31.0 34.0 30.0 34.0 4 35.62803810661877 37.0 35.0 37.0 33.0 37.0 5 35.63554604059222 37.0 35.0 37.0 33.0 37.0 6 35.65361776680262 37.0 35.0 37.0 33.0 37.0 7 35.59268823549755 37.0 35.0 37.0 33.0 37.0 8 35.56533782575648 37.0 35.0 37.0 33.0 37.0 9 37.19763485547402 39.0 37.0 39.0 34.0 39.0 10-11 37.10847174710768 39.0 37.0 39.0 33.5 39.0 12-13 37.00429295474931 39.0 37.0 39.0 33.0 39.0 14-15 38.19482476091386 40.0 38.0 41.0 33.0 41.0 16-17 38.040360433809724 40.0 38.0 41.0 33.0 41.0 18-19 38.18220343348082 40.0 38.0 41.0 33.5 41.0 20-21 38.15947323419883 40.0 38.0 41.0 33.0 41.0 22-23 38.070621009559034 40.0 38.0 41.0 33.0 41.0 24-25 37.96137002963145 40.0 38.0 41.0 33.0 41.0 26-27 37.79040421506826 40.0 38.0 41.0 32.5 41.0 28-29 37.58191970149276 40.0 37.5 41.0 32.0 41.0 30-31 37.36812477879427 40.0 37.0 41.0 31.5 41.0 32-33 37.12224316037406 40.0 36.5 41.0 31.0 41.0 34-35 36.8918229485905 40.0 36.0 41.0 30.5 41.0 36-37 36.631882947310174 39.0 36.0 41.0 30.0 41.0 38-39 36.34861231805944 39.0 35.0 41.0 30.0 41.0 40-41 36.133670068850634 39.0 35.0 40.0 30.0 41.0 42-43 35.957213753348896 39.0 35.0 40.5 28.5 41.0 44-45 35.69204446704228 39.0 35.0 40.0 27.5 41.0 46-47 35.30859569589759 39.0 35.0 40.0 26.0 41.0 48-49 34.92053977508942 38.0 34.5 40.0 24.5 41.0 50-51 34.23148898956535 37.5 33.5 39.5 23.5 40.5 52-53 34.35493098381271 38.0 34.0 40.0 24.0 40.5 54-55 34.45343660804883 38.0 34.0 40.0 23.5 41.0 56-57 34.15060900098526 38.0 34.0 40.0 22.5 41.0 58-59 33.8242451044091 38.0 33.5 40.0 21.5 41.0 60-61 33.404638164454184 37.0 33.0 40.0 19.5 41.0 62-63 32.85367990675696 36.5 32.0 39.5 17.0 41.0 64-65 32.32712970069071 36.0 31.5 39.0 12.0 40.5 66-67 31.789244195672055 35.5 31.0 39.0 8.0 40.0 68-69 31.172433590453185 35.0 30.5 38.0 4.5 40.0 70-71 30.61133298112108 35.0 29.5 37.5 2.0 40.0 72-73 29.954512927980197 34.5 29.0 37.0 2.0 39.0 74-75 29.253363982667036 34.0 28.5 36.0 2.0 39.0 76-77 28.578930434891383 34.0 27.5 36.0 2.0 38.5 78-79 27.844234376161214 33.5 26.0 35.0 2.0 37.0 80-81 27.17178901259649 33.0 25.5 35.0 2.0 37.0 82-83 26.439716150280823 32.5 23.5 35.0 2.0 36.0 84-85 25.76176189029079 32.0 21.0 35.0 2.0 36.0 86-87 25.06296940683385 31.5 19.0 34.5 2.0 35.0 88-89 24.2487023823627 31.0 12.0 34.0 2.0 35.0 90-91 23.406577784580023 30.5 4.5 34.0 2.0 35.0 92-93 22.495947842172612 30.0 2.0 34.0 2.0 35.0 94-95 21.53476563046116 29.0 2.0 34.0 2.0 35.0 96-97 20.322758013257996 28.5 2.0 34.0 2.0 35.0 98-99 18.824600873123387 26.0 2.0 33.5 2.0 35.0 100-101 15.167568720019956 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28528156388352244 1101 2 0.16249025717552712 1101 3 0.25052164167735214 1101 4 0.11321462156554674 1101 5 0.2119297865081009 1101 6 0.20037286110171948 1101 7 0.15670643700732967 1101 8 0.08160316001137602 1101 9 0.18989217077735532 1101 10-11 0.1994910620697965 1101 12-13 0.23965357581239743 1101 14-15 0.384334000330675 1101 16-17 0.510535769866884 1101 18-19 0.4266476616084489 1101 20-21 0.5299677254052568 1101 22-23 0.5947711629063974 1101 24-25 0.4826590902635388 1101 26-27 0.6622614746700819 1101 28-29 0.6986343542112721 1101 30-31 0.7445083861958253 1101 32-33 0.6634345648412321 1101 34-35 0.7048500703720038 1101 36-37 0.7633738950005196 1101 38-39 0.8520537429736734 1101 40-41 0.9111089735433708 1101 42-43 1.0448104672184826 1101 44-45 1.1109460415562893 1101 46-47 1.124554108348697 1101 48-49 1.1986037423923577 1101 50-51 1.10434473237531 1101 52-53 1.1101314778461528 1101 54-55 1.2533629026222357 1101 56-57 1.151741790660239 1101 58-59 1.427658979824436 1101 60-61 1.2992555371218586 1101 62-63 1.333943641164339 1101 64-65 1.453781289331559 1101 66-67 1.5358048146215033 1101 68-69 1.6698731242832139 1101 70-71 2.08077827519557 1101 72-73 2.2131320065129927 1101 74-75 2.3727333767794683 1101 76-77 2.350150559971322 1101 78-79 2.499429110011004 1101 80-81 2.6766821369903475 1101 82-83 2.804566877352652 1101 84-85 2.85550436161898 1101 86-87 3.0958243688102023 1101 88-89 3.5754824007457806 1101 90-91 3.8291600437980122 1101 92-93 4.08925030056778 1101 94-95 4.362464618318683 1101 96-97 4.819113708958387 1101 98-99 5.159419909018517 1101 100-101 4.91996822832413 1102 1 0.30923383013962535 1102 2 0.22316760147951697 1102 3 0.10673855661541154 1102 4 0.08137711186449792 1102 5 0.23118302165472215 1102 6 0.18385726701268368 1102 7 -0.0031733077139222132 1102 8 0.04338133741219252 1102 9 0.22186932528830994 1102 10-11 0.29280569237523224 1102 12-13 0.28742122627171796 1102 14-15 0.324659034509331 1102 16-17 0.36230791106218874 1102 18-19 0.31984541891950613 1102 20-21 0.4514844957590327 1102 22-23 0.38312530482336626 1102 24-25 0.35871921455412803 1102 26-27 0.2803724353644199 1102 28-29 0.42107807140876474 1102 30-31 0.4972565341021209 1102 32-33 0.5362883002980396 1102 34-35 0.5128640750385571 1102 36-37 0.603800230270302 1102 38-39 0.528035337988662 1102 40-41 0.7350963184417694 1102 42-43 0.9222104449489876 1102 44-45 0.7435598022630145 1102 46-47 0.7195839084506659 1102 48-49 0.9661236823819195 1102 50-51 0.7578523035853664 1102 52-53 0.8809956933612995 1102 54-55 0.9868079183155345 1102 56-57 1.044093474560647 1102 58-59 0.9207766171816019 1102 60-61 0.9888929378195854 1102 62-63 1.0501288764884436 1102 64-65 1.0451884452071525 1102 66-67 1.1852170589932207 1102 68-69 1.3525644514865718 1102 70-71 1.5305548686156811 1102 72-73 1.6616959170365035 1102 74-75 1.7364052985448843 1102 76-77 1.933670044524579 1102 78-79 2.067212181677764 1102 80-81 2.203038336742704 1102 82-83 2.3936422662944246 1102 84-85 2.3591972468498703 1102 86-87 2.6698648339915074 1102 88-89 3.151501586000361 1102 90-91 3.1499894736905425 1102 92-93 3.1285601471175575 1102 94-95 3.587464471808836 1102 96-97 3.7769343624927494 1102 98-99 3.917634464729513 1102 100-101 3.744995709384593 1103 1 0.4788030417021716 1103 2 0.5111465115725977 1103 3 0.45109351246872365 1103 4 0.36985696613528773 1103 5 0.4844548623890219 1103 6 0.42619549070231244 1103 7 0.3497414728874517 1103 8 0.33295494597692255 1103 9 0.4672756357716281 1103 10-11 0.5049077538625966 1103 12-13 0.6275558452047392 1103 14-15 0.7305429693551773 1103 16-17 0.695980703433392 1103 18-19 0.6877021551661144 1103 20-21 0.7676443621215725 1103 22-23 0.7825563445180919 1103 24-25 0.8362511222641871 1103 26-27 0.8527462080315544 1103 28-29 0.9610617396863503 1103 30-31 0.9693837605752194 1103 32-33 1.1101922886145772 1103 34-35 1.0439895505625643 1103 36-37 1.0679111721317227 1103 38-39 1.0920781655751384 1103 40-41 1.157833530820902 1103 42-43 1.2722200633912735 1103 44-45 1.2093716380506834 1103 46-47 1.2633046359974927 1103 48-49 1.499476121736663 1103 50-51 1.5575076100210907 1103 52-53 1.5067605558175714 1103 54-55 1.6472833834359264 1103 56-57 1.6666863121581557 1103 58-59 1.8338410136341565 1103 60-61 1.7949031883309274 1103 62-63 1.905535029935976 1103 64-65 2.006533103438379 1103 66-67 2.1888325136707216 1103 68-69 2.3521968895850236 1103 70-71 2.4497344351183727 1103 72-73 2.495346724396768 1103 74-75 2.433713999505553 1103 76-77 2.566069472914293 1103 78-79 2.7919611207046877 1103 80-81 2.764023088567267 1103 82-83 2.90043965871509 1103 84-85 3.0375512369330586 1103 86-87 3.1606909992952374 1103 88-89 3.53782435736802 1103 90-91 3.751624314436615 1103 92-93 3.983862755089479 1103 94-95 4.220396938566324 1103 96-97 4.470338859212667 1103 98-99 4.616802469471992 1103 100-101 4.523596441956563 1104 1 0.21263987133693973 1104 2 0.23543280375937314 1104 3 0.23405554436885723 1104 4 0.196230925913369 1104 5 0.12853498837144883 1104 6 0.17458675861724515 1104 7 0.1381408314172674 1104 8 0.1503078701563041 1104 9 0.3187644533860521 1104 10-11 0.26025225513397743 1104 12-13 0.3228575095598032 1104 14-15 0.48344114318782516 1104 16-17 0.5663943361194796 1104 18-19 0.40838776254013 1104 20-21 0.4097635975584666 1104 22-23 0.3574151199457347 1104 24-25 0.4293448433691367 1104 26-27 0.453470390301554 1104 28-29 0.5553712201532974 1104 30-31 0.5085369188045235 1104 32-33 0.4778836461041749 1104 34-35 0.5083745915935367 1104 36-37 0.5804805202593215 1104 38-39 0.6028188180254332 1104 40-41 0.7840644600858084 1104 42-43 0.7748473461004721 1104 44-45 0.5891050089475911 1104 46-47 0.715420115077471 1104 48-49 0.6758876450838684 1104 50-51 0.7082606873442501 1104 52-53 0.7167437966867425 1104 54-55 0.905148616907951 1104 56-57 0.885372743041188 1104 58-59 0.8690781697830232 1104 60-61 0.857074507101153 1104 62-63 0.9347038637316274 1104 64-65 1.0991471666607566 1104 66-67 1.0866151795283265 1104 68-69 1.1088337221092956 1104 70-71 1.3262929620485693 1104 72-73 1.5838569676931158 1104 74-75 1.848522714253587 1104 76-77 1.8845077675282553 1104 78-79 2.110342347588645 1104 80-81 2.106990755002773 1104 82-83 2.267459928594885 1104 84-85 2.5708495350144283 1104 86-87 2.6688267627025404 1104 88-89 2.929095884182633 1104 90-91 3.119898268435694 1104 92-93 3.4006099667168463 1104 94-95 3.8512489092089517 1104 96-97 4.137938113720292 1104 98-99 4.336975550840464 1104 100-101 4.162697589090175 1105 1 0.3153022678172661 1105 2 0.28604098181320836 1105 3 0.10657754229846716 1105 4 0.0776556153543666 1105 5 0.10877243661161629 1105 6 0.18173932072905075 1105 7 0.2353296254131294 1105 8 0.16524705235092085 1105 9 0.18870169458687513 1105 10-11 0.21201694198698107 1105 12-13 0.1371432238455199 1105 14-15 0.19986195064123535 1105 16-17 0.3628390824962864 1105 18-19 0.2834281999107304 1105 20-21 0.3899832533555667 1105 22-23 0.3098850345420061 1105 24-25 0.28607523933186485 1105 26-27 0.21823973553964748 1105 28-29 0.40844284282410825 1105 30-31 0.4204918230488275 1105 32-33 0.44055671805034535 1105 34-35 0.4500364057757338 1105 36-37 0.5755374560771287 1105 38-39 0.5119916311724353 1105 40-41 0.7187435491127232 1105 42-43 0.6687235106967506 1105 44-45 0.7190205757177779 1105 46-47 0.7792642532762315 1105 48-49 0.706212438044524 1105 50-51 0.8021449394146458 1105 52-53 0.7292049768109621 1105 54-55 0.9892842276739984 1105 56-57 1.053113426270997 1105 58-59 0.9991393110873759 1105 60-61 1.0058290360866593 1105 62-63 1.0574943658020217 1105 64-65 1.0586208338450263 1105 66-67 1.3623058498181884 1105 68-69 1.5186050083411828 1105 70-71 1.5275432855475302 1105 72-73 1.562045049991255 1105 74-75 1.8045139150817455 1105 76-77 1.813478717321214 1105 78-79 1.95740012450376 1105 80-81 2.1532608119385905 1105 82-83 2.372191100955135 1105 84-85 2.326829413378814 1105 86-87 2.5725842031787316 1105 88-89 2.9366107651350113 1105 90-91 2.933300830547495 1105 92-93 3.258919037627823 1105 94-95 3.486015342956364 1105 96-97 3.430474992602278 1105 98-99 3.6426238427659285 1105 100-101 3.3970645016160574 1106 1 0.0816066156433557 1106 2 0.1215482281900151 1106 3 -0.04595868958558924 1106 4 0.05482952839784616 1106 5 0.11312026269857256 1106 6 0.027753813482675582 1106 7 0.06685136454355955 1106 8 0.13481226974222693 1106 9 0.06297705690571576 1106 10-11 0.04045897097248741 1106 12-13 0.18134612239624914 1106 14-15 0.24152861730789965 1106 16-17 0.1467158940904909 1106 18-19 0.10643544628754142 1106 20-21 0.11715716639904628 1106 22-23 0.1542690925130259 1106 24-25 0.14024190599853625 1106 26-27 0.19831219930775745 1106 28-29 0.17202979934584306 1106 30-31 0.2078106636285426 1106 32-33 0.3026943992097628 1106 34-35 0.296775536210518 1106 36-37 0.34111716622205535 1106 38-39 0.049853950013009296 1106 40-41 0.3754464476634567 1106 42-43 0.3426365541750016 1106 44-45 0.29601332934096547 1106 46-47 0.6600613547255065 1106 48-49 0.699328380073517 1106 50-51 0.6271449394146487 1106 52-53 0.6616325130428464 1106 54-55 0.21627698129719164 1106 56-57 0.43680907844490946 1106 58-59 0.6386320647105634 1106 60-61 0.6750319346373885 1106 62-63 0.7218059600049145 1106 64-65 0.8908672106566158 1106 66-67 1.019189907789201 1106 68-69 0.7207789213846567 1106 70-71 0.9818911116344893 1106 72-73 1.2182044702811083 1106 74-75 1.358318262907833 1106 76-77 1.4935511810893338 1106 78-79 1.9246102694312945 1106 80-81 1.9452897974458345 1106 82-83 2.113676608201512 1106 84-85 2.4204888336686707 1106 86-87 2.1303740582511956 1106 88-89 2.566139750642261 1106 90-91 2.7452573522866253 1106 92-93 3.058013240526371 1106 94-95 3.2825733139708575 1106 96-97 3.317793833181991 1106 98-99 3.632841234070277 1106 100-101 3.5581152262537366 1107 1 0.29084099373238104 1107 2 0.1736495253903172 1107 3 0.15404739341698104 1107 4 0.12028263605178324 1107 5 0.15396572401827768 1107 6 0.2408721887126859 1107 7 0.2729161181059183 1107 8 0.26037304654165183 1107 9 0.24721447649602624 1107 10-11 0.2467440432841812 1107 12-13 0.2558604326597873 1107 14-15 0.4381939913365258 1107 16-17 0.4243893737200395 1107 18-19 0.31332045472770886 1107 20-21 0.42391709309886494 1107 22-23 0.43139301087912685 1107 24-25 0.518731078253964 1107 26-27 0.5414165456130959 1107 28-29 0.5969100402327499 1107 30-31 0.6540167709631177 1107 32-33 0.5605644738786211 1107 34-35 0.5276665440119643 1107 36-37 0.6643291436541148 1107 38-39 0.7297654043068533 1107 40-41 0.7774656243290536 1107 42-43 0.8076326762608659 1107 44-45 0.6640119834766409 1107 46-47 0.90025251308127 1107 48-49 1.0519471126799331 1107 50-51 0.967017690604635 1107 52-53 0.8510112103678438 1107 54-55 0.9475616734212124 1107 56-57 0.9764807103624236 1107 58-59 0.9384602198879577 1107 60-61 1.096961158902488 1107 62-63 1.2706226259112157 1107 64-65 1.3439355151717223 1107 66-67 1.4986350619172804 1107 68-69 1.6532726102694895 1107 70-71 1.8599396463912399 1107 72-73 1.9538151277016098 1107 74-75 1.9761100949561303 1107 76-77 2.1084600501070803 1107 78-79 2.3298775793754842 1107 80-81 2.4071788358752038 1107 82-83 2.5992674958636606 1107 84-85 2.702411480687239 1107 86-87 2.8154051195830654 1107 88-89 3.107920663700945 1107 90-91 3.433462030115603 1107 92-93 3.480149476972681 1107 94-95 3.8138735937889763 1107 96-97 4.021382234416841 1107 98-99 4.092689805325048 1107 100-101 4.048636767686588 1108 1 0.21687542562559514 1108 2 0.16861870685387714 1108 3 0.10708240057993379 1108 4 0.16006990511988306 1108 5 0.10529365334619456 1108 6 0.1682585225081823 1108 7 0.06639464130888939 1108 8 0.043579564776479174 1108 9 0.061026281521009196 1108 10-11 0.15388696989784023 1108 12-13 0.15607130486165488 1108 14-15 0.2836915600383989 1108 16-17 0.3009543901874494 1108 18-19 0.2761053294201261 1108 20-21 0.2605255375687676 1108 22-23 0.18688319869371384 1108 24-25 0.18401774593644404 1108 26-27 0.21222789207929793 1108 28-29 0.24293309640997762 1108 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-1.4611043974155322 2316 56-57 -1.3246811152928757 2316 58-59 -1.323160650890145 2316 60-61 -1.494529303657437 2316 62-63 -1.514858650795233 2316 64-65 -1.422601699336223 2316 66-67 -1.6943162509679048 2316 68-69 -1.69428317462652 2316 70-71 -1.829747192916713 2316 72-73 -1.9430205608884812 2316 74-75 -1.927543012525227 2316 76-77 -2.145819436378016 2316 78-79 -2.9891937689095087 2316 80-81 -3.0908044761922078 2316 82-83 -3.005545964770697 2316 84-85 -2.8866252381849726 2316 86-87 -2.750036297126332 2316 88-89 -3.027986550301229 2316 90-91 -3.0923130556149765 2316 92-93 -3.2274428302484885 2316 94-95 -3.3505439323175317 2316 96-97 -3.5472609678189713 2316 98-99 -3.276801995718124 2316 100-101 -3.038962127381831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8360.0 3 2536.0 4 2699.0 5 2936.0 6 3447.0 7 4195.0 8 5220.0 9 6586.0 10 8090.0 11 10023.0 12 12361.0 13 14988.0 14 17559.0 15 21394.0 16 25267.0 17 30531.0 18 30360.0 19 25835.0 20 26513.0 21 28231.0 22 31373.0 23 34623.0 24 38667.0 25 43456.0 26 49468.0 27 56290.0 28 65200.0 29 76085.0 30 88895.0 31 106125.0 32 130660.0 33 164471.0 34 213709.0 35 286996.0 36 377644.0 37 426545.0 38 227329.0 39 13390.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46919609246883 18.43196839794696 14.75395718658051 23.344878323003705 2 15.492081158600973 19.369334056189157 40.667927615779305 24.47065716943056 3 19.342956203828756 23.493026161235903 29.435264643662624 27.728752991272714 4 12.858191991606924 16.420758137694296 36.65488077599139 34.06616909470739 5 14.056468301016988 36.78389165862747 34.10275198279734 15.056888057558195 6 32.782733503545515 35.594327471642146 17.115124459372584 14.507814565439759 7 29.12801791535261 32.42217981902078 20.09396431652812 18.35583794909849 8 26.48694427646488 35.00618896836173 19.1849185717267 19.32194818344669 9 26.287989852809048 17.12570362826122 20.06654125001702 36.51976526891271 10-11 25.25037936267071 26.36098026025798 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36-37 22.729300468445018 26.074613312937245 28.72946021161922 22.46662600699852 38-39 22.2931005169037 26.398374288917005 28.686039331197417 22.62248586298188 40-41 22.74122070617069 26.1973152670611 28.734720012953545 22.326744013814665 42-43 22.463755849332674 26.25023735889106 28.581377667803537 22.704629123972733 44-45 22.434385940122915 26.446239633445856 28.63019817974017 22.489176246691056 46-47 22.705483882067472 26.717960239568733 28.008579841861376 22.56797603650242 48-49 22.700523276204386 26.599720384513613 28.02035694951943 22.67939938976257 50-51 22.62491083612669 26.868458057884347 27.881573081626733 22.62505802436223 52-53 22.774224920835756 26.76572921900663 27.83832629375192 22.621719566405694 54-55 22.631205164860845 26.76697594748298 27.88973338558542 22.712085502070764 56-57 22.62723576996444 26.98810950121282 27.779607393495514 22.605047335327228 58-59 22.720643551395785 26.701020856288814 27.74412046792298 22.834215124392426 60-61 22.468066864329366 26.865304492855348 27.964328721101488 22.702299921713795 62-63 22.430736028304466 26.819786263025087 28.12182106036869 22.627656648301762 64-65 22.40487699780705 26.770186712521298 28.01876379792488 22.806172491746775 66-67 22.372423420569366 26.82459225277513 27.79585613211279 23.007128194542716 68-69 22.50689916547694 26.617642729935387 28.029892704178504 22.845565400409164 70-71 22.521857346900138 26.56352774699552 27.870506845375704 23.04410806072864 72-73 22.553590385652072 26.65288001751542 27.69285823362851 23.100671363203997 74-75 22.47639902210853 26.970611741468105 27.539110507329035 23.01387872909433 76-77 22.635810428817717 26.77844635698941 27.53728079817896 23.048462416013916 78-79 22.699555924009044 26.82738180986586 27.571715503618822 22.901346762506275 80-81 22.77828088265652 26.942811755653672 27.38269019112302 22.896217170566782 82-83 22.78038103203674 26.928181030233706 27.36881981111959 22.922618126609965 84-85 22.84468746061694 26.890759549213993 27.23573907692081 23.028813913248257 86-87 22.823982391156576 26.76821982724128 27.25559037547566 23.152207406126486 88-89 22.853183556711446 26.877172890205724 27.138793747154793 23.130849805928037 90-91 22.95264803354918 26.83161557556386 27.100826032632796 23.11491035825416 92-93 22.98711231568948 26.472878864010063 27.261241513404595 23.27876730689586 94-95 22.8553416205987 26.50502487688527 27.248147988669142 23.391485513846888 96-97 23.054928919196417 26.22728928764633 27.189557396414866 23.528224396742388 98-99 22.969209940520415 26.164466588135802 26.982195868628985 23.8841276027148 100-101 23.775567520804135 25.260333813118223 27.00428711413716 23.959811551940476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 797.0 1 544.5 2 392.5 3 507.0 4 440.0 5 415.5 6 756.0 7 1734.0 8 2977.5 9 3588.0 10 3093.0 11 2443.5 12 2406.0 13 2536.5 14 2746.5 15 2958.0 16 3201.0 17 3424.0 18 3603.0 19 3979.0 20 4476.5 21 5139.5 22 6217.5 23 7654.0 24 9455.5 25 11904.0 26 15364.0 27 19167.0 28 22168.5 29 25485.5 30 29822.5 31 34862.0 32 41149.0 33 45801.0 34 48995.0 35 53406.0 36 59028.5 37 64452.5 38 68420.0 39 71665.0 40 74639.0 41 78853.5 42 83113.5 43 86623.5 44 90334.0 45 94326.0 46 96342.5 47 98439.0 48 100819.0 49 101280.5 50 100362.0 51 97962.0 52 96086.5 53 95584.0 54 96584.5 55 92437.0 56 84260.0 57 77675.0 58 72027.5 59 63747.0 60 52944.5 61 44372.0 62 36941.5 63 29020.0 64 22366.0 65 17406.0 66 12816.0 67 9734.5 68 7270.5 69 4937.5 70 3561.5 71 2526.5 72 1732.0 73 1271.5 74 921.0 75 645.0 76 473.0 77 294.0 78 170.5 79 118.5 80 75.5 81 52.5 82 34.5 83 24.5 84 18.0 85 13.0 86 10.5 87 10.0 88 7.0 89 3.0 90 4.5 91 5.0 92 2.0 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4566127727958712 2 0.038888059048040914 3 0.05169131784531456 4 0.056400562460403716 5 0.0808665598747341 6 0.08211745297561715 7 0.04716602809800233 8 0.0414634271969178 9 0.04587834402356388 10-11 0.04895039031543845 12-13 0.045160920039233894 14-15 0.03883287258770784 16-17 0.03886966356126322 18-19 0.04611748535167388 20-21 0.055168064846298354 22-23 0.04624625375911772 24-25 0.06583744717735972 26-27 0.055885488830628334 28-29 0.043744467557351605 30-31 0.04578636658967542 32-33 0.04459065994912544 34-35 0.04917113615677075 36-37 0.04403879534579468 38-39 0.026636664854098033 40-41 0.02411648316555423 42-43 0.024061296705221155 44-45 0.015599372787482827 46-47 0.027905953441758783 48-49 0.027777185034314943 50-51 0.01653754261314512 52-53 0.016500751639589736 54-55 0.017034220756142804 56-57 0.015672954734593594 58-59 0.014293293226266697 60-61 0.017126198190031267 62-63 0.017126198190031267 64-65 0.017825226687583563 66-67 0.009528862150844463 68-69 0.012435349061719803 70-71 0.018156345449582018 72-73 0.016666311020588964 74-75 0.013759824109713627 76-77 0.009988749320286764 78-79 0.014440457120488229 80-81 0.01799078606858279 82-83 0.01517627659159591 84-85 0.013263145966715942 86-87 0.0038814477100930153 88-89 0.02821867671697955 90-91 0.03138270044274257 92-93 0.017052616242920496 94-95 0.03226568380807179 96-97 0.026783828748319572 98-99 0.024098087678776536 100-101 0.1469615438669815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.98041094981359 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43238794066647 42.78716486794086 2 13.385128271183191 14.986099624736058 3 4.390512944398127 7.373481567236495 4 1.9648764985211034 4.3997837541136855 5 1.030428186736824 2.8841896673899323 6 0.6235278360211025 2.0943206699465575 7 0.41055557310744045 1.6088148790203525 8 0.28870038951804633 1.292925315727319 9 0.21392916062242218 1.0778258093212683 >10 1.1352072078475008 11.570859501728044 >50 0.08073763134131086 3.10780401704103 >100 0.03952789540498847 3.8358660641472064 >500 0.0033603484735341997 1.3038491713981974 >1k 9.224486005479792E-4 0.7577293141923362 >5k 1.3177837150685415E-4 0.5184560794571133 >10k+ 6.588918575342708E-5 0.4008296966036177 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10867 0.39980750962635825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8779 0.3229879568427164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5277 0.19414596745176152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.358194711076805E-5 0.0 12-13 0.0 0.0 0.0 1.8395486777692013E-4 0.0 14-15 0.0 0.0 0.0 2.2074584133230417E-4 3.679097355538403E-5 16-17 0.0 0.0 0.0 2.2074584133230417E-4 3.679097355538403E-5 18-19 0.0 3.679097355538403E-5 0.0 2.2074584133230417E-4 7.358194711076805E-5 20-21 0.0 3.679097355538403E-5 0.0 2.759323016653802E-4 7.358194711076805E-5 22-23 0.0 3.679097355538403E-5 0.0 4.7828265621999237E-4 1.287684074438441E-4 24-25 0.0 3.679097355538403E-5 0.0 7.726104446630646E-4 1.8395486777692013E-4 26-27 0.0 3.679097355538403E-5 0.0 0.001361266021549209 1.8395486777692013E-4 28-29 0.0 3.679097355538403E-5 0.0 0.0036790973555384026 1.8395486777692013E-4 30-31 0.0 3.679097355538403E-5 0.0 0.012932027204717485 1.8395486777692013E-4 32-33 0.0 3.679097355538403E-5 0.0 0.029487965304640297 2.575368148876882E-4 34-35 0.0 3.679097355538403E-5 0.0 0.05465299121652297 2.943277884430722E-4 36-37 0.0 3.679097355538403E-5 0.0 0.09589567257210846 2.943277884430722E-4 38-39 0.0 3.679097355538403E-5 0.0 0.1602798762940305 3.1272327522076425E-4 40-41 0.0 3.679097355538403E-5 0.0 0.22630127833916716 4.047007091092243E-4 42-43 0.0 3.679097355538403E-5 0.0 0.29521077180840144 4.047007091092243E-4 44-45 0.0 3.679097355538403E-5 0.0 0.37854232691134626 4.2309619588691636E-4 46-47 0.0 3.679097355538403E-5 0.0 0.4723225185040202 4.4149168266460835E-4 48-49 0.0 3.679097355538403E-5 0.0 0.5676663264727979 4.7828265621999237E-4 50-51 0.0 3.679097355538403E-5 0.0 0.6695957187079893 4.7828265621999237E-4 52-53 0.0 3.679097355538403E-5 0.0 0.7685634375719723 5.150736297753764E-4 54-55 0.0 3.679097355538403E-5 0.0 0.8739879722949253 5.518646033307605E-4 56-57 0.0 3.679097355538403E-5 0.0 0.9950118798053611 5.518646033307605E-4 58-59 0.0 3.679097355538403E-5 0.0 1.1275881530121874 5.886555768861445E-4 60-61 0.0 3.679097355538403E-5 0.0 1.2638251280877744 6.254465504415285E-4 62-63 0.0 3.679097355538403E-5 0.0 1.3984616958137024 6.622375239969125E-4 64-65 0.0 3.679097355538403E-5 0.0 1.5367037789480578 6.990284975522965E-4 66-67 0.0 3.679097355538403E-5 0.0 1.699614209851298 6.990284975522965E-4 68-69 0.0 3.679097355538403E-5 0.0 1.8775721489386907 6.990284975522965E-4 70-71 0.0 3.679097355538403E-5 0.0 2.0613430618478343 8.829833653292167E-4 72-73 0.0 3.679097355538403E-5 0.0 2.2486091172447384 9.381698256622926E-4 74-75 0.0 3.679097355538403E-5 0.0 2.433998832990319 9.749607992176768E-4 76-77 0.0 3.679097355538403E-5 0.0 2.6330931863852793 9.933562859953688E-4 78-79 0.0 3.679097355538403E-5 0.0 2.860700544285663 9.933562859953688E-4 80-81 0.0 3.679097355538403E-5 0.0 3.1019573533750937 9.933562859953688E-4 82-83 0.0 3.679097355538403E-5 0.0 3.340712376262758 9.933562859953688E-4 84-85 0.0 3.679097355538403E-5 0.0 3.5828337732307407 9.933562859953688E-4 86-87 0.0 3.679097355538403E-5 0.0 3.8318350822535794 9.933562859953688E-4 88-89 0.0 3.679097355538403E-5 0.0 4.102119969478208 0.0010301472595507528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5490 0.0 51.05368 1 GTATCAA 9450 0.0 39.866352 1 ATCAACG 11770 0.0 31.41551 3 TATCAAC 11895 0.0 31.16529 2 TCAACGC 11885 0.0 31.15152 4 CAACGCA 12195 0.0 30.20375 5 AACGCAG 12590 0.0 29.442715 6 ACGCAGA 14390 0.0 25.619696 7 CGCAGAG 14550 0.0 25.402803 8 GTACATG 9155 0.0 24.35678 1 TACATGG 8990 0.0 23.578499 2 GCAGAGT 15570 0.0 23.522898 9 GGACCGA 740 0.0 22.477362 6 GAGTACT 9440 0.0 22.168228 12-13 ACATGGG 9350 0.0 21.857365 3 CAGAGTA 15205 0.0 20.651333 10-11 GTACTTT 10110 0.0 20.510778 14-15 AGAGTAC 13825 0.0 20.479258 10-11 TGGTATC 2765 0.0 20.282797 2 ACCGAGT 715 0.0 19.93335 8 >>END_MODULE