FastQCFastQC Report
Thu 26 May 2016
SRR938178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718058
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106420.39152954057639683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89320.32861697579669014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57730.21239429033523197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55050.048.321571
GTATCAA92150.040.413931
TATCAAC119700.031.032942
ATCAACG119700.030.791443
TCAACGC122300.030.3304624
CAACGCA124600.029.7705885
AACGCAG129100.028.6960936
CGCAGAG146200.025.1123058
ACGCAGA146650.025.0352447
GCAGAGT161500.022.4097429
TGGTATC28950.022.3151952
GTGGTAT31700.021.8778151
GTACATG90100.021.5629841
GAGTACT98350.021.53847512-13
AGAGTAC142350.021.2537910-11
ATTCGCG4500.021.10998380-81
AGTACTT101700.021.01580212-13
CGCGGAA4300.020.9872582-83
TACATGG90150.020.918722
TTTACGC1150.00372611820.650693
Produced by FastQC (version 0.11.5)