##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938178_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718058 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50040212534096 33.0 31.0 34.0 31.0 34.0 2 32.73038250103566 34.0 31.0 34.0 31.0 34.0 3 32.69433580887531 34.0 31.0 34.0 31.0 34.0 4 36.21725106675428 37.0 35.0 37.0 35.0 37.0 5 36.17723977928359 37.0 35.0 37.0 35.0 37.0 6 36.23580806590588 37.0 37.0 37.0 35.0 37.0 7 36.19841923903022 37.0 37.0 37.0 35.0 37.0 8 36.19423720906618 37.0 37.0 37.0 35.0 37.0 9 37.900963114105735 39.0 38.0 39.0 35.0 39.0 10-11 37.868174814518305 39.0 38.0 39.0 35.0 39.0 12-13 37.80796638629492 39.0 38.0 39.0 35.0 39.0 14-15 39.09708273333388 40.0 38.5 41.0 36.0 41.0 16-17 39.03379968345047 40.0 38.0 41.0 36.0 41.0 18-19 39.02385913030554 40.0 38.5 41.0 36.0 41.0 20-21 39.02520991089962 40.0 38.5 41.0 35.5 41.0 22-23 39.0516994118595 40.0 39.0 41.0 35.5 41.0 24-25 39.00398464639092 40.0 38.5 41.0 35.0 41.0 26-27 38.841728358997486 40.0 38.0 41.0 35.0 41.0 28-29 38.764631034363504 40.0 38.0 41.0 35.0 41.0 30-31 38.55907029945645 40.0 38.0 41.0 34.0 41.0 32-33 38.372180063854415 40.0 38.0 41.0 34.0 41.0 34-35 38.16585315692306 40.0 38.0 41.0 34.0 41.0 36-37 38.09120279994025 40.0 38.0 41.0 34.0 41.0 38-39 38.054477866182395 40.0 38.0 41.0 33.0 41.0 40-41 37.93926270153176 40.0 38.0 41.0 33.0 41.0 42-43 37.75779490356718 40.0 37.0 41.0 33.0 41.0 44-45 37.51203046439774 40.0 37.0 41.0 33.0 41.0 46-47 37.16780951694187 40.0 37.0 41.0 31.5 41.0 48-49 36.8105251985057 40.0 36.0 41.0 31.0 41.0 50-51 36.51672738403669 39.0 36.0 41.0 30.5 41.0 52-53 36.05980427937888 39.0 35.0 40.5 29.5 41.0 54-55 35.74362302055364 39.0 35.0 40.0 28.5 41.0 56-57 35.4701818357077 38.0 35.0 40.0 28.0 41.0 58-59 35.089156669945964 38.0 34.0 40.0 27.5 41.0 60-61 34.67360299154764 38.0 34.0 40.0 26.0 41.0 62-63 34.241516737317596 37.0 34.0 40.0 26.0 41.0 64-65 33.802212461985725 36.5 33.0 39.0 25.5 41.0 66-67 33.27682006785727 36.0 32.5 39.0 23.5 40.0 68-69 32.74599659021257 35.0 32.0 38.5 22.0 40.0 70-71 32.15016272647603 35.0 31.0 38.0 20.5 40.0 72-73 31.676460914373422 35.0 31.0 37.0 19.5 39.0 74-75 31.11485884407176 34.5 30.5 36.5 18.0 39.0 76-77 29.907503445474674 33.5 29.5 35.0 17.5 37.5 78-79 30.59672953998774 34.0 30.0 36.0 17.5 38.0 80-81 30.401603461000462 34.0 30.0 35.0 14.0 37.0 82-83 29.937399054766306 34.0 30.0 35.0 7.5 37.0 84-85 29.427166197336483 34.0 29.0 35.0 4.5 36.0 86-87 28.877493232300417 34.0 29.0 35.0 2.0 36.0 88-89 28.244455048420598 33.5 27.5 35.0 2.0 35.0 90-91 27.70545440899348 33.0 27.0 35.0 2.0 35.0 92-93 27.06215908564129 33.0 25.5 35.0 2.0 35.0 94-95 26.3036877800253 32.0 24.5 34.5 2.0 35.0 96-97 25.46850453522331 32.0 21.5 34.0 2.0 35.0 98-99 24.44141478952988 32.0 10.0 34.0 2.0 35.0 100-101 20.9302836804807 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12115990570474366 1101 2 0.05639613633388052 1101 3 0.1632505053826918 1101 4 0.04183024649778844 1101 5 0.04582627453625321 1101 6 0.05419595307898106 1101 7 0.11561189509092884 1101 8 0.11436411343770203 1101 9 0.10417472158174945 1101 10-11 0.0975074132488416 1101 12-13 0.13012919134185807 1101 14-15 0.23895748540530093 1101 16-17 0.3254033196423123 1101 18-19 0.3443333457838804 1101 20-21 0.21625454232004415 1101 22-23 0.18886574800754374 1101 24-25 0.24952538123023515 1101 26-27 0.2543779494761509 1101 28-29 0.28203279020644345 1101 30-31 0.30656613437929536 1101 32-33 0.22522844243036388 1101 34-35 0.16152168828607216 1101 36-37 0.2840977009680259 1101 38-39 0.34363865446768216 1101 40-41 0.3932254308701246 1101 42-43 0.4207650183914282 1101 44-45 0.23072783477908843 1101 46-47 0.35795435826656075 1101 48-49 0.4058067299459722 1101 50-51 0.49250211784723064 1101 52-53 0.4451124430337643 1101 54-55 0.4163649258847002 1101 56-57 0.49432523982139287 1101 58-59 0.5376852787813107 1101 60-61 0.5816685976922429 1101 62-63 0.6494936225277073 1101 64-65 0.7007896754112011 1101 66-67 0.7036823510566634 1101 68-69 0.6914121991813218 1101 70-71 0.7576648886672643 1101 72-73 0.7808377658881049 1101 74-75 0.8209260341875577 1101 76-77 0.8926229449986955 1101 78-79 0.9740009473973821 1101 80-81 1.1536448398004495 1101 82-83 1.1696485931749976 1101 84-85 1.2919743016612308 1101 86-87 1.4357636950139003 1101 88-89 1.6520071625494062 1101 90-91 1.845641989948124 1101 92-93 1.9548814512488804 1101 94-95 2.049107187492801 1101 96-97 2.1848370705109623 1101 98-99 2.286125218052092 1101 100-101 2.5990159417995393 1102 1 0.05321265929443797 1102 2 0.060711562815662035 1102 3 0.12552681624811157 1102 4 0.06268805743981432 1102 5 -0.0015789524326095261 1102 6 0.03227121560368573 1102 7 0.05858758534066055 1102 8 0.04663554343236598 1102 9 0.018713772315180677 1102 10-11 0.135146210394673 1102 12-13 0.0733310090685464 1102 14-15 0.15247222303966623 1102 16-17 0.16554927693344013 1102 18-19 0.12247030988226726 1102 20-21 0.16675908035776388 1102 22-23 0.2741199090963775 1102 24-25 0.07637084013673245 1102 26-27 0.09575090576850442 1102 28-29 0.033890911749331565 1102 30-31 0.06831475865187286 1102 32-33 0.13695952738179074 1102 34-35 0.1223747606811969 1102 36-37 0.03990355114790134 1102 38-39 0.21042344992738293 1102 40-41 0.18509672992685466 1102 42-43 0.10950711837141824 1102 44-45 0.1191744034767197 1102 46-47 0.18414496003689607 1102 48-49 0.25982289960939653 1102 50-51 0.29097456436904423 1102 52-53 0.3488095142601537 1102 54-55 0.3483585732179293 1102 56-57 0.45298752961116406 1102 58-59 0.3935854812997661 1102 60-61 0.3761452603487285 1102 62-63 0.33890690502631315 1102 64-65 0.41457323767662047 1102 66-67 0.445396800861964 1102 68-69 0.4974408699017161 1102 70-71 0.6519497490099013 1102 72-73 0.7616823993162818 1102 74-75 0.7469144523752078 1102 76-77 0.8700038345897205 1102 78-79 0.9688104804160993 1102 80-81 0.9839532890649316 1102 82-83 1.037911547365976 1102 84-85 1.0355156873429472 1102 86-87 1.122561316363818 1102 88-89 1.2207768733557245 1102 90-91 1.3688737626586125 1102 92-93 1.46956232350988 1102 94-95 1.5652482187044185 1102 96-97 1.8262752446132104 1102 98-99 1.9196756371120998 1102 100-101 2.3525272804055533 1103 1 0.2438156374782423 1103 2 0.25424031410629766 1103 3 0.31801442023525084 1103 4 0.18812765951111032 1103 5 0.16378542949414765 1103 6 0.1655539703255542 1103 7 0.1760110990950352 1103 8 0.22125168906735837 1103 9 0.2654523352021272 1103 10-11 0.29162639051471473 1103 12-13 0.25095674566455983 1103 14-15 0.32134959378939243 1103 16-17 0.3506691541314524 1103 18-19 0.4167503792920897 1103 20-21 0.4088332876214551 1103 22-23 0.43053040458356406 1103 24-25 0.5263329552518243 1103 26-27 0.5502648014731832 1103 28-29 0.5356398978870018 1103 30-31 0.6019122719901091 1103 32-33 0.47674003060298276 1103 34-35 0.46655746789973307 1103 36-37 0.4086976929276602 1103 38-39 0.4711422223785888 1103 40-41 0.5205129140356064 1103 42-43 0.5081351162828369 1103 44-45 0.5682356238803834 1103 46-47 0.6156952173793044 1103 48-49 0.623942772881513 1103 50-51 0.536945222231239 1103 52-53 0.5927546066953511 1103 54-55 0.6248248949292972 1103 56-57 0.6401487739627854 1103 58-59 0.6883370506762176 1103 60-61 0.5712402473736446 1103 62-63 0.6011695356113833 1103 64-65 0.6787500364234802 1103 66-67 0.6728425351809619 1103 68-69 0.7239309432766845 1103 70-71 0.6558060573339759 1103 72-73 0.6651052888533862 1103 74-75 0.8262397088342865 1103 76-77 0.5484034632000707 1103 78-79 0.8573604119092337 1103 80-81 1.0439089657125322 1103 82-83 1.1289759163372715 1103 84-85 1.2073188311995118 1103 86-87 1.2414221002079735 1103 88-89 1.3961738794683392 1103 90-91 1.349261660695273 1103 92-93 1.6017507350535816 1103 94-95 1.566420098305077 1103 96-97 1.8222810392339532 1103 98-99 2.200492341009337 1103 100-101 2.6499038993464232 1104 1 0.041430350839313235 1104 2 0.0753467056920627 1104 3 0.12950309337441013 1104 4 0.0840274514667314 1104 5 0.008145115115965496 1104 6 0.07984618599823534 1104 7 0.04473003691287403 1104 8 0.04440746229898451 1104 9 0.05680476816559832 1104 10-11 0.09605166790723274 1104 12-13 0.019005679437100298 1104 14-15 0.13444952733077287 1104 16-17 0.11104320059470041 1104 18-19 0.16730985975903678 1104 20-21 0.2708886649908493 1104 22-23 0.2335506600158297 1104 24-25 0.13817703754492783 1104 26-27 0.15972215237470522 1104 28-29 0.13803854848801933 1104 30-31 0.16693589369607054 1104 32-33 0.28128919553594756 1104 34-35 0.2805870997767599 1104 36-37 0.21951275013987015 1104 38-39 0.2986625933910716 1104 40-41 0.27531491068378955 1104 42-43 0.2816394360932861 1104 44-45 0.33213667277081527 1104 46-47 0.16188648818374674 1104 48-49 0.2873786114159529 1104 50-51 0.33097261474163986 1104 52-53 0.3720841692242445 1104 54-55 0.45110386222010135 1104 56-57 0.5454510162189123 1104 58-59 0.4821033041436209 1104 60-61 0.6103031085618014 1104 62-63 0.3503120749086577 1104 64-65 0.5236934021192781 1104 66-67 0.6106278738309854 1104 68-69 0.7389050822040986 1104 70-71 0.6751823576113694 1104 72-73 0.6857161302773385 1104 74-75 0.6562197712475992 1104 76-77 0.6645222074210579 1104 78-79 0.8172042341468675 1104 80-81 0.9461024614360625 1104 82-83 0.9533167479369027 1104 84-85 1.1715146096322435 1104 86-87 1.155995320272698 1104 88-89 1.3487527629634855 1104 90-91 1.2159496374636483 1104 92-93 1.356840237480764 1104 94-95 1.4148276196874185 1104 96-97 1.796453918337047 1104 98-99 2.0296228888595422 1104 100-101 2.48060561571258 1105 1 0.022143342557740198 1105 2 0.048218082503652226 1105 3 0.07008280351933394 1105 4 0.05497724442739127 1105 5 0.04266892463977712 1105 6 0.01527255763384261 1105 7 0.040922454097142236 1105 8 0.03863947575653981 1105 9 0.10127617085711194 1105 10-11 0.08896704057804072 1105 12-13 0.04609710024455893 1105 14-15 0.058807381885635834 1105 16-17 0.05845197388662626 1105 18-19 0.13279090271969807 1105 20-21 0.08739843465959041 1105 22-23 0.0764692676762877 1105 24-25 0.13487734810393448 1105 26-27 0.009321013658343702 1105 28-29 0.05315080262880656 1105 30-31 0.0761313517706057 1105 32-33 -0.06095168179324162 1105 34-35 0.04348338600864565 1105 36-37 0.14201695562640992 1105 38-39 0.14423389256291586 1105 40-41 0.12252149712270466 1105 42-43 0.1813499045197844 1105 44-45 0.20686655113519947 1105 46-47 0.2611117081630354 1105 48-49 0.321438199925268 1105 50-51 0.3288634014911267 1105 52-53 0.2906352173608866 1105 54-55 0.298611302696294 1105 56-57 0.26829004313402294 1105 58-59 0.3990465624251982 1105 60-61 0.42035907388271454 1105 62-63 0.48039424364571914 1105 64-65 0.5454325325540594 1105 66-67 0.45678794111876897 1105 68-69 0.579740356531218 1105 70-71 0.6936120936362187 1105 72-73 0.6879806230309562 1105 74-75 0.5717283116202694 1105 76-77 0.60579582284549 1105 78-79 0.8329812786603235 1105 80-81 1.0099336596762285 1105 82-83 0.9309581169845202 1105 84-85 1.1341585666715837 1105 86-87 1.2979790159248772 1105 88-89 1.2833995020939213 1105 90-91 1.3671999609626155 1105 92-93 1.6101350744372844 1105 94-95 1.6734343096460087 1105 96-97 1.7753132609871507 1105 98-99 1.810167661116271 1105 100-101 2.059626707845087 1106 1 0.06996942951426632 1106 2 0.06742098105437577 1106 3 0.1548654122149884 1106 4 0.06548449080420227 1106 5 0.04230660579919743 1106 6 -0.00900280468499659 1106 7 0.01266158453192645 1106 8 -0.024404002504333278 1106 9 0.05671095346581012 1106 10-11 0.058532257969339696 1106 12-13 0.05714782488223591 1106 14-15 0.044133468842154855 1106 16-17 0.11805342316198164 1106 18-19 -0.005252575541170756 1106 20-21 -0.004087072586784757 1106 22-23 0.11505622419802819 1106 24-25 0.09321068143727018 1106 26-27 0.13540797018008988 1106 28-29 0.0343102229186556 1106 30-31 0.1350081633648017 1106 32-33 0.25263527472849745 1106 34-35 0.10181671934197567 1106 36-37 0.190929999104668 1106 38-39 0.18517592154842077 1106 40-41 0.21237656958648188 1106 42-43 0.146929614664721 1106 44-45 0.18748249316418253 1106 46-47 -0.09649698748913238 1106 48-49 0.09698167818613967 1106 50-51 0.1448054304766302 1106 52-53 0.013823623157989573 1106 54-55 0.10694463602962401 1106 56-57 0.2760798982064898 1106 58-59 0.11245235952664245 1106 60-61 0.15948950866532385 1106 62-63 0.1483290262544159 1106 64-65 0.22568615574245854 1106 66-67 0.21838214401731904 1106 68-69 -0.03964370143979323 1106 70-71 0.17042368783911144 1106 72-73 0.13272699984255 1106 74-75 0.3905688913304175 1106 76-77 0.39710017067157466 1106 78-79 0.6416769308342403 1106 80-81 0.6961655437341996 1106 82-83 0.6438204358251021 1106 84-85 0.8803542188454934 1106 86-87 1.0740659724466148 1106 88-89 1.1828560238330503 1106 90-91 1.1088666276292827 1106 92-93 1.2831423208140968 1106 94-95 1.604774889356154 1106 96-97 1.7715089131610675 1106 98-99 2.223211139377142 1106 100-101 2.699300620888568 1107 1 0.1213354437789036 1107 2 0.11143340558712822 1107 3 0.21782068693859458 1107 4 0.12646393025551816 1107 5 0.08384590959955318 1107 6 0.08049146968883036 1107 7 0.07062765658508852 1107 8 0.07674074254182273 1107 9 0.037964280107836146 1107 10-11 0.0697960777908051 1107 12-13 0.05064585551014744 1107 14-15 0.161833485570412 1107 16-17 0.17988790466205273 1107 18-19 0.17707402086681867 1107 20-21 0.0792907427028382 1107 22-23 0.08984445967105614 1107 24-25 0.20979142569263587 1107 26-27 0.24180785916528436 1107 28-29 0.21931638195876246 1107 30-31 0.19000820138357 1107 32-33 0.16706168560490653 1107 34-35 0.21335560493589867 1107 36-37 0.3461575794383265 1107 38-39 0.3929813643149771 1107 40-41 0.4198153213543492 1107 42-43 0.32701443453179735 1107 44-45 0.3739054215216271 1107 46-47 0.30100289845456274 1107 48-49 0.4307672447417943 1107 50-51 0.44608685298678097 1107 52-53 0.42511044433292255 1107 54-55 0.4785955629271399 1107 56-57 0.4952883094782905 1107 58-59 0.6665867102421572 1107 60-61 0.79294279120559 1107 62-63 0.7554147737793926 1107 64-65 0.6497645808530947 1107 66-67 0.707761221529978 1107 68-69 0.8161131001174553 1107 70-71 0.813209436884847 1107 72-73 0.67368176511469 1107 74-75 0.7347335810212492 1107 76-77 0.8496126408268445 1107 78-79 1.0500150165133313 1107 80-81 1.0731708435501872 1107 82-83 1.0629929193629764 1107 84-85 1.2700433393953965 1107 86-87 1.3529883685413289 1107 88-89 1.4144389731768463 1107 90-91 1.4150825057867387 1107 92-93 1.6841741501250453 1107 94-95 1.7731187538876867 1107 96-97 1.8672122223144605 1107 98-99 1.9641833932816688 1107 100-101 2.202792834645276 1108 1 0.09198804173235686 1108 2 0.05956078911121665 1108 3 0.16503347442487382 1108 4 0.0955935222822859 1108 5 -0.002104504814397501 1108 6 -0.004456095032615792 1108 7 0.021441193182802465 1108 8 0.030676297201637226 1108 9 0.06553832402357784 1108 10-11 0.06653573563318815 1108 12-13 0.0573814104581416 1108 14-15 0.17975041834885985 1108 16-17 0.11090182388438308 1108 18-19 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Count 2 5.0 3 7.0 4 29.0 5 36.0 6 106.0 7 196.0 8 539.0 9 1057.0 10 1856.0 11 3063.0 12 4798.0 13 7059.0 14 9767.0 15 13090.0 16 17087.0 17 23799.0 18 23938.0 19 18341.0 20 16717.0 21 17227.0 22 18212.0 23 19884.0 24 22692.0 25 26255.0 26 31248.0 27 37450.0 28 45047.0 29 54629.0 30 66886.0 31 82443.0 32 103447.0 33 135001.0 34 187201.0 35 276343.0 36 426628.0 37 603591.0 38 395631.0 39 26752.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.751663135959575 18.87884658826265 15.120464684712395 23.249025591065386 2 15.704365302032217 19.70010004514726 40.070307564318284 24.52522708850224 3 19.606525795184577 23.4304098287974 29.5536134451308 27.409450930887218 4 13.153324910653122 16.75185003410523 36.33822383481147 33.75660122043018 5 14.06909309381787 36.84426626068829 34.110497883955475 14.976142761538359 6 32.8547309415968 35.77097282503321 16.92034702781734 14.453949205552652 7 29.10449298727253 32.47016803909262 19.95038369306321 18.474955280571642 8 26.518474968874813 35.15692833407406 19.04938676760155 19.275209929449577 9 26.093004637870127 17.035030157561025 20.039454640040795 36.83251056452805 10-11 25.269549067753523 26.40445862450323 26.699135927195076 21.62685638054817 12-13 26.000751271680002 23.59002272946346 27.437641139372303 22.971584859484235 14-15 22.75203472479248 25.201209834374396 25.724929342935287 26.321826097897837 16-17 22.36876887262319 28.466487555910792 26.483637671388482 22.681105900077537 18-19 22.28440305541677 26.790911010728983 28.87883555097058 22.04585038288366 20-21 22.719732250010853 25.96169765325096 29.31300582989767 22.005564266840516 22-23 22.53222705328584 25.784199601333015 29.220826045654658 22.462747299726495 24-25 22.386390352069693 26.066873116500915 29.26148624013204 22.285250291297356 26-27 22.175000699184384 26.45568348585366 29.03791860904995 22.33139720591201 28-29 22.043497344236734 26.587562190702087 29.089520395312668 22.279420069748507 30-31 22.337347416195144 26.190359247631257 29.043722617143025 22.42857071903057 32-33 22.136739652563975 26.259562290652426 29.04114397226163 22.562554084521967 34-35 22.612650205179836 26.37064986370407 28.82798747137197 22.188712459744128 36-37 22.714933342065788 26.069097011550756 28.80874491460286 22.4072247317806 38-39 22.230769768598027 26.381436618277455 28.826128923167392 22.561664689957126 40-41 22.635333367556864 26.198665392668108 28.796617831895116 22.369383407879916 42-43 22.445929118350982 26.231406004833772 28.655616983570457 22.66704789324478 44-45 22.397239671534894 26.438865365917373 28.687344600443193 22.47655036210454 46-47 22.687236663375035 26.75947128848964 28.036106642963 22.517185405172324 48-49 22.63253241659836 26.62883197230958 28.094937109370978 22.643698501721087 50-51 22.589584181058683 26.832245669518457 27.96689768945328 22.61127245996958 52-53 22.805878314590785 26.75652984594148 27.874331599987933 22.563260239479803 54-55 22.603859078798173 26.73539343163391 27.904205870514904 22.756541619053014 56-57 22.58018408731528 26.982371237111202 27.829593776144584 22.60785089942893 58-59 22.672474244478963 26.705776697921824 27.79558052109263 22.82616853650658 60-61 22.457633354402297 26.903031502639017 27.936397972375865 22.70293717058282 62-63 22.394279297939924 26.88452564294066 28.094249644415243 22.626945414704174 64-65 22.28197485116212 26.878510318764352 27.981374937547322 22.858139892526207 66-67 22.277584614413897 26.898341292775836 27.77867813714278 23.04539595566749 68-69 22.421474509027703 26.7151769255207 27.965999895881254 22.89734866957034 70-71 22.441242975683373 26.68795147123424 27.73399243136092 23.136813121721463 72-73 22.500237301779432 26.7659115441981 27.606290962150183 23.127560191872284 74-75 22.403293086460994 27.11148915880382 27.382804193288003 23.10241356144718 76-77 22.45608813351297 26.913130624879972 27.565655331858263 23.065125909748797 78-79 22.531987911957728 26.99624143414158 27.563263918577164 22.908506735323527 80-81 22.590122414831498 27.119062799732014 27.38546672930955 22.90534805612694 82-83 22.572897019697542 27.109786703933864 27.440614607015856 22.87670166935274 84-85 22.59516036250095 27.09816565895308 27.30727535057193 22.999398627974045 86-87 22.632471620122626 26.988357397899588 27.30233422306924 23.076836758908552 88-89 22.53039579376003 27.23432584379209 27.12969227650487 23.10558608594301 90-91 22.651037268828954 27.071309127634564 27.19007489086464 23.087578712671846 92-93 22.56032694105153 26.84320541595453 27.281001941286075 23.315465701707865 94-95 22.443834858442948 26.864347165768475 27.253945466907233 23.437872508881348 96-97 22.555602321074065 26.6568667560444 27.27526920014775 23.512261722733783 98-99 22.389261725212865 26.641625457634564 27.10109037608906 23.86802244106351 100-101 22.699409413475323 26.42679699894333 27.1070458311626 23.766747756418745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 758.0 1 520.5 2 374.0 3 498.5 4 432.5 5 405.0 6 808.0 7 1998.0 8 3318.0 9 3790.5 10 3238.0 11 2515.0 12 2406.0 13 2567.5 14 2792.0 15 2962.0 16 3136.5 17 3389.5 18 3629.0 19 4008.5 20 4494.5 21 5067.0 22 6097.5 23 7581.5 24 9279.5 25 11662.5 26 15031.0 27 18719.0 28 21761.0 29 25286.5 30 29912.5 31 35081.0 32 41556.5 33 45992.0 34 49065.0 35 53500.5 36 59198.0 37 64932.0 38 68692.5 39 71924.0 40 75329.0 41 79422.5 42 82858.0 43 86454.0 44 90684.0 45 94770.5 46 97539.0 47 100435.5 48 102383.5 49 101942.0 50 101111.0 51 98986.5 52 96629.0 53 96378.0 54 98881.5 55 94328.5 56 84663.0 57 77561.5 58 71947.5 59 63639.0 60 51870.5 61 42726.5 62 35718.5 63 27636.0 64 20649.0 65 15766.5 66 11211.0 67 8620.5 68 6351.0 69 4110.0 70 2972.0 71 2007.0 72 1380.5 73 1032.5 74 747.5 75 521.0 76 345.0 77 193.5 78 103.0 79 63.5 80 35.5 81 22.5 82 12.5 83 6.5 84 4.0 85 3.5 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010485427463284448 3 0.006144092583749133 4 0.0 5 5.518646033307604E-4 6 1.8395486777692015E-4 7 0.0 8 7.358194711076805E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8395486777692013E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016555938099922813 26-27 0.02229532997456272 28-29 2.575368148876882E-4 30-31 9.197743388846008E-5 32-33 0.0033111876199845625 34-35 0.01626161031147974 36-37 0.008351550997072174 38-39 0.02130197368856735 40-41 0.023159917853114245 42-43 0.01891056040746739 44-45 0.009768003478954458 46-47 0.0019867125719907376 48-49 2.2074584133230417E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.759323016653802E-4 68-69 2.759323016653802E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8395486777692015E-4 82-83 0.001048542746328445 84-85 0.003918238683648398 86-87 8.461923917738327E-4 88-89 3.679097355538403E-5 90-91 0.003789470276204555 92-93 5.334691165530684E-4 94-95 7.358194711076805E-5 96-97 2.2074584133230417E-4 98-99 1.8395486777692015E-4 100-101 0.0030168598315414904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.31564443732114 #Duplication Level Percentage of deduplicated Percentage of total 1 74.99404826268533 40.73350060351318 2 14.163420214245374 15.38590592746637 3 4.704049740101281 7.665104792964423 4 2.0729378504267504 4.503718208977768 5 1.077558219569263 2.9264134557318457 6 0.6860438088374383 2.235774695354389 7 0.44475967506689323 1.6910185859694302 8 0.29889638915946404 1.2987799997747695 9 0.22171306576398878 1.0838239242430694 >10 1.1970738411115076 11.872967094219556 >50 0.09070514788567853 3.3527324262930964 >100 0.04424729485007192 4.213346021079782 >500 0.003257187078478487 1.2334377379864803 >1k 0.0010857290261457291 0.8692483374389091 >5k 1.3571612826821614E-4 0.5419901968300185 >10k+ 6.785806413410807E-5 0.3922379921567533 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10642 0.39152954057639683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8932 0.32861697579669014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5773 0.21239429033523197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1037292066615209E-4 7 0.0 0.0 0.0 0.0 1.1037292066615209E-4 8 0.0 0.0 0.0 0.0 1.1037292066615209E-4 9 0.0 0.0 0.0 3.679097355538403E-5 1.471638942215361E-4 10-11 0.0 0.0 0.0 7.358194711076805E-5 1.471638942215361E-4 12-13 0.0 0.0 0.0 1.8395486777692013E-4 1.471638942215361E-4 14-15 0.0 0.0 0.0 2.3914132810999618E-4 2.0235035455461214E-4 16-17 0.0 0.0 0.0 2.575368148876882E-4 2.575368148876882E-4 18-19 0.0 0.0 0.0 2.575368148876882E-4 2.575368148876882E-4 20-21 0.0 0.0 0.0 3.3111876199845623E-4 2.575368148876882E-4 22-23 0.0 0.0 0.0 5.518646033307604E-4 3.1272327522076425E-4 24-25 0.0 0.0 0.0 7.910059314407566E-4 3.6790973555384025E-4 26-27 0.0 0.0 0.0 0.001416452481882285 3.6790973555384025E-4 28-29 0.0 0.0 0.0 0.0036790973555384026 3.6790973555384025E-4 30-31 0.0 0.0 0.0 0.012950422691495177 3.6790973555384025E-4 32-33 0.0 0.0 0.0 0.029727106632750293 4.7828265621999237E-4 34-35 0.0 0.0 0.0 0.05513127387274297 4.7828265621999237E-4 36-37 0.0 0.0 0.0 0.09681544691099307 4.7828265621999237E-4 38-39 0.0 0.0 0.0 0.16188028364368973 4.7828265621999237E-4 40-41 0.0 0.0 0.0 0.22797526763593712 4.7828265621999237E-4 42-43 0.0 0.0 0.0 0.2970135295126153 4.7828265621999237E-4 44-45 0.0 0.0 0.0 0.3806945988643362 4.7828265621999237E-4 46-47 0.0 0.0 0.0 0.4745299769173432 5.150736297753764E-4 48-49 0.0 0.0 0.0 0.5700761352406756 5.518646033307605E-4 50-51 0.0 0.0 0.0 0.6723734372114208 5.518646033307605E-4 52-53 0.0 0.0 0.0 0.7718562297051792 5.702600901084524E-4 54-55 0.0 0.0 0.0 0.8782189342537945 5.886555768861444E-4 56-57 0.0 0.0 0.0 0.9999786612353379 5.886555768861444E-4 58-59 0.0 0.0 0.0 1.1334379178074934 5.886555768861444E-4 60-61 0.0 0.0 0.0 1.2706130627087429 5.886555768861444E-4 62-63 0.0 0.0 0.0 1.4063717551281099 5.886555768861444E-4 64-65 0.0 0.0 0.0 1.5451289118922409 5.886555768861444E-4 66-67 0.0 0.0 0.0 1.7083152750971466 5.886555768861444E-4 68-69 0.0 0.0 0.0 1.8874321298515337 5.886555768861444E-4 70-71 0.0 0.0 0.0 2.073704828962443 6.254465504415285E-4 72-73 0.0 0.0 0.0 2.2624793142751187 8.094014182184487E-4 74-75 0.0 0.0 0.0 2.449561414804246 8.094014182184487E-4 76-77 0.0 0.0 0.0 2.6503481529827546 8.277969049961406E-4 78-79 0.0 0.0 0.0 2.880861997794013 8.829833653292167E-4 80-81 0.0 0.0 0.0 3.1265889101704234 9.197743388846007E-4 82-83 0.0 0.0 0.0 3.370145155107066 9.933562859953688E-4 84-85 0.0 0.0 0.0 3.6182634807645755 0.0010117517727730608 86-87 0.0 0.0 0.0 3.873703210159607 0.0010301472595507528 88-89 0.0 0.0 0.0 4.153259422720192 0.0010301472595507528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5505 0.0 48.32157 1 GTATCAA 9215 0.0 40.41393 1 TATCAAC 11970 0.0 31.03294 2 ATCAACG 11970 0.0 30.79144 3 TCAACGC 12230 0.0 30.330462 4 CAACGCA 12460 0.0 29.770588 5 AACGCAG 12910 0.0 28.696093 6 CGCAGAG 14620 0.0 25.112305 8 ACGCAGA 14665 0.0 25.035244 7 GCAGAGT 16150 0.0 22.409742 9 TGGTATC 2895 0.0 22.315195 2 GTGGTAT 3170 0.0 21.877815 1 GTACATG 9010 0.0 21.562984 1 GAGTACT 9835 0.0 21.538475 12-13 AGAGTAC 14235 0.0 21.25379 10-11 ATTCGCG 450 0.0 21.109983 80-81 AGTACTT 10170 0.0 21.015802 12-13 CGCGGAA 430 0.0 20.98725 82-83 TACATGG 9015 0.0 20.91872 2 TTTACGC 115 0.003726118 20.65069 3 >>END_MODULE