FastQCFastQC Report
Thu 26 May 2016
SRR938177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749671
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112770.41012179275266025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89090.32400239883244214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56820.20664290382376654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.048.101491
GTATCAA97650.042.3047521
ATCAACG122250.033.194543
TATCAAC123250.033.0023162
TCAACGC123700.032.8822634
CAACGCA125450.032.3856855
AACGCAG130600.031.1813766
ACGCAGA148100.027.34897
CGCAGAG149500.026.9973958
GCAGAGT163100.024.4543119
TGGTATC29500.023.1951982
GTACATG89950.022.7772561
GAGTACT96500.022.53614212-13
TACATGG87350.022.1949862
GTGGTAT34300.021.024621
GTACTTT103750.020.94759414-15
CAGAGTA155050.020.92561710-11
AGAGTAC145250.020.89739610-11
AGTACTT100850.020.7156612-13
ACATGGG88450.020.4147193
Produced by FastQC (version 0.11.5)