##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938177_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2749671 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.871145311566366 33.0 31.0 34.0 30.0 34.0 2 32.064160039510185 34.0 31.0 34.0 30.0 34.0 3 32.13718695800334 34.0 31.0 34.0 30.0 34.0 4 35.64536993698519 37.0 35.0 37.0 33.0 37.0 5 35.664902091923 37.0 35.0 37.0 35.0 37.0 6 35.692687234218205 37.0 36.0 37.0 35.0 37.0 7 35.63088129452578 37.0 35.0 37.0 33.0 37.0 8 35.61267111592623 37.0 35.0 37.0 33.0 37.0 9 37.27391640672648 39.0 37.0 39.0 34.0 39.0 10-11 37.193105829752 39.0 37.0 39.0 34.0 39.0 12-13 37.096418444242964 39.0 37.0 39.0 33.5 39.0 14-15 38.35537797067359 40.0 38.0 41.0 33.5 41.0 16-17 38.272947563544875 40.0 38.0 41.0 33.0 41.0 18-19 38.20566951464375 40.0 38.0 41.0 33.0 41.0 20-21 38.09950917764344 40.0 38.0 41.0 33.0 41.0 22-23 37.94947995596564 40.0 38.0 41.0 33.0 41.0 24-25 37.90268217543117 40.0 38.0 41.0 33.0 41.0 26-27 37.919978972029746 40.0 38.0 41.0 33.0 41.0 28-29 37.83300438488823 40.0 38.0 41.0 33.0 41.0 30-31 37.65419681118214 40.0 37.5 41.0 32.5 41.0 32-33 37.44106149426604 40.0 37.0 41.0 32.0 41.0 34-35 37.23689815981621 40.0 37.0 41.0 31.0 41.0 36-37 37.02150148144996 40.0 36.5 41.0 31.0 41.0 38-39 36.79010597995178 40.0 36.0 41.0 30.0 41.0 40-41 36.56609790771333 39.0 36.0 41.0 30.0 41.0 42-43 36.28188863322194 39.0 35.0 41.0 30.0 41.0 44-45 36.060511239344635 39.0 35.0 41.0 29.0 41.0 46-47 35.923115529094204 39.0 35.0 41.0 28.0 41.0 48-49 35.67743486402556 39.0 35.0 41.0 27.0 41.0 50-51 35.03875681854302 38.5 34.5 40.5 26.5 40.5 52-53 35.1104841270101 38.5 35.0 40.0 26.0 41.0 54-55 35.24509186735432 39.0 35.0 40.5 26.0 41.0 56-57 35.03957127961854 39.0 35.0 40.5 26.0 41.0 58-59 34.7771753420682 38.0 34.5 40.0 25.5 41.0 60-61 34.40526866668776 38.0 34.0 40.0 24.0 41.0 62-63 33.98889267115956 37.5 34.0 40.0 23.0 41.0 64-65 33.56168938029313 37.0 33.5 40.0 22.0 41.0 66-67 33.127434700369605 36.0 33.0 39.0 20.5 41.0 68-69 32.64102378066322 36.0 32.5 39.0 19.0 40.5 70-71 32.16353101880189 35.0 32.0 38.5 17.5 40.0 72-73 31.6773184864662 35.0 31.0 37.5 13.0 40.0 74-75 31.15189089894755 35.0 31.0 37.0 8.0 39.0 76-77 30.61278967556482 35.0 30.5 36.5 7.0 39.0 78-79 30.05430849727113 34.0 30.0 36.0 2.0 38.5 80-81 29.53145885453205 34.0 29.0 35.5 2.0 37.0 82-83 29.000070735735292 34.0 29.0 35.0 2.0 37.0 84-85 28.506141280174973 34.0 28.5 35.0 2.0 36.0 86-87 28.014644661124912 34.0 27.0 35.0 2.0 36.0 88-89 27.468776446345764 33.0 26.0 35.0 2.0 35.5 90-91 26.87058597192173 33.0 25.0 35.0 2.0 35.0 92-93 26.260529896122115 32.5 24.5 35.0 2.0 35.0 94-95 25.647445094340377 32.0 21.5 35.0 2.0 35.0 96-97 24.80318790866253 32.0 17.0 34.0 2.0 35.0 98-99 23.74099883222393 31.0 2.0 34.0 2.0 35.0 100-101 20.841266282402515 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5264122850769155 1101 2 0.4709597994346879 1101 3 0.3680850308280199 1101 4 0.34319923434067334 1101 5 0.31610065901347895 1101 6 0.29800619741569534 1101 7 0.3686489718353556 1101 8 0.27578466096790777 1101 9 0.24170802004569936 1101 10-11 0.3594495849258905 1101 12-13 0.27423422630072025 1101 14-15 0.3946527653564118 1101 16-17 0.4514471222617473 1101 18-19 0.42945518759788825 1101 20-21 0.5433745899525135 1101 22-23 0.44764819327517813 1101 24-25 0.44107510190627863 1101 26-27 0.38196798827588196 1101 28-29 0.46457868020262794 1101 30-31 0.4894276556184636 1101 32-33 0.5527903273993928 1101 34-35 0.5944926836273154 1101 36-37 0.5035406734406394 1101 38-39 0.6123846928358816 1101 40-41 0.6241467158969272 1101 42-43 0.6431796443323563 1101 44-45 0.6522760738229536 1101 46-47 0.6430078681500575 1101 48-49 0.6711046470096491 1101 50-51 0.7413019019909655 1101 52-53 0.7043748197576676 1101 54-55 0.6963772554812024 1101 56-57 0.6206270823500475 1101 58-59 0.6082698834104718 1101 60-61 0.627135474250025 1101 62-63 0.6642818287352199 1101 64-65 0.6446785229122796 1101 66-67 0.5483694888776967 1101 68-69 0.6112257323633443 1101 70-71 0.7138068644413167 1101 72-73 0.8502976068011101 1101 74-75 0.8302606277438009 1101 76-77 0.8340634725187925 1101 78-79 0.8576661776520176 1101 80-81 0.8652038159744215 1101 82-83 0.8159508426999729 1101 84-85 0.8209267103858942 1101 86-87 0.9540254319029948 1101 88-89 0.8864634686064612 1101 90-91 0.9349896286273278 1101 92-93 1.0404304390638366 1101 94-95 1.2838170189255003 1101 96-97 1.3463995387808758 1101 98-99 1.6900176120583055 1101 100-101 1.4660424586696301 1102 1 0.5030941702519698 1102 2 0.3705768216765932 1102 3 0.34870009001713953 1102 4 0.24969565360219548 1102 5 0.28995286819580457 1102 6 0.27540807683080004 1102 7 0.22468595915589873 1102 8 0.291226400447691 1102 9 0.4672664347564677 1102 10-11 0.5185655174778816 1102 12-13 0.359910664176887 1102 14-15 0.3623290513433872 1102 16-17 0.38731700516569134 1102 18-19 0.4376295057649813 1102 20-21 0.47166513623088946 1102 22-23 0.433415731792401 1102 24-25 0.49648030250028796 1102 26-27 0.5144976403674377 1102 28-29 0.5512336855927273 1102 30-31 0.6134692660253123 1102 32-33 0.45110642854182004 1102 34-35 0.4755946759072671 1102 36-37 0.5288576763840638 1102 38-39 0.5658591746290682 1102 40-41 0.5786186691623953 1102 42-43 0.7049072327034267 1102 44-45 0.5803889228927588 1102 46-47 0.6201967081735873 1102 48-49 0.5035323985465467 1102 50-51 0.6637620565563083 1102 52-53 0.5571258059689512 1102 54-55 0.5557366545786095 1102 56-57 0.595251559326833 1102 58-59 0.5810250054790842 1102 60-61 0.5852396998271843 1102 62-63 0.5540037794527635 1102 64-65 0.5494282164215818 1102 66-67 0.6580119542037295 1102 68-69 0.6294385455649447 1102 70-71 0.7228816685552104 1102 72-73 0.7527838389660495 1102 74-75 0.772419364446506 1102 76-77 0.6516720001437193 1102 78-79 0.6083595419681167 1102 80-81 0.7410947266553549 1102 82-83 0.8086388737984649 1102 84-85 0.8879169963814171 1102 86-87 0.6211047801997118 1102 88-89 0.6693121373239599 1102 90-91 0.6807454434027278 1102 92-93 0.8735724314621471 1102 94-95 1.0595596896964636 1102 96-97 1.0189203503952058 1102 98-99 1.0264328659417572 1102 100-101 1.1124887939482626 1103 1 0.39443755692170157 1103 2 0.3662485150114634 1103 3 0.37197624979611277 1103 4 0.17754186057813826 1103 5 0.18433527869424893 1103 6 0.2180465020639062 1103 7 0.29504396537208066 1103 8 0.3462971958891572 1103 9 0.3919124575491395 1103 10-11 0.38588449986536233 1103 12-13 0.33773384824503694 1103 14-15 0.46036634845400926 1103 16-17 0.4358953406094628 1103 18-19 0.5187679885553607 1103 20-21 0.4733573199536849 1103 22-23 0.5339395029446976 1103 24-25 0.6788332436767632 1103 26-27 0.5667999311467611 1103 28-29 0.5630962388332037 1103 30-31 0.6545133548937372 1103 32-33 0.7219594272755643 1103 34-35 0.7033948370676129 1103 36-37 0.7258186525553612 1103 38-39 0.7226739553358712 1103 40-41 0.8848175870857276 1103 42-43 0.7445699471042602 1103 44-45 0.7416759034383986 1103 46-47 0.8382581793373944 1103 48-49 0.9781611541583786 1103 50-51 0.8057270617364622 1103 52-53 0.9807703322726198 1103 54-55 0.9846131366782984 1103 56-57 0.8903937257824523 1103 58-59 0.9524190425459693 1103 60-61 0.8769054072060243 1103 62-63 0.8477127698982585 1103 64-65 0.8033708893811777 1103 66-67 0.819713347089646 1103 68-69 0.8592244325224669 1103 70-71 1.0638221543385669 1103 72-73 1.2318387486011382 1103 74-75 1.1045305650911494 1103 76-77 1.1634827518416593 1103 78-79 1.0961861794724364 1103 80-81 0.9858397479515446 1103 82-83 0.9526414063183211 1103 84-85 1.220845510252719 1103 86-87 1.2695277110158756 1103 88-89 1.2495297038026045 1103 90-91 1.4038546871002069 1103 92-93 1.3529899518949762 1103 94-95 1.4974323730163697 1103 96-97 1.2881387226756225 1103 98-99 1.5705390015466811 1103 100-101 1.4634845347103216 1104 1 0.41452774149014004 1104 2 0.36663380988764516 1104 3 0.2299910744326894 1104 4 0.23440867891502393 1104 5 0.24226626601980428 1104 6 0.325154018212622 1104 7 0.3288250503963468 1104 8 0.335613438699248 1104 9 0.24098701404010825 1104 10-11 0.41407249165703064 1104 12-13 0.2867380943670028 1104 14-15 0.345923885153276 1104 16-17 0.34926865522309214 1104 18-19 0.42844488045056295 1104 20-21 0.36574841506369893 1104 22-23 0.3590439465451496 1104 24-25 0.40369420232073594 1104 26-27 0.36750912939302083 1104 28-29 0.4443533228258474 1104 30-31 0.351710007644499 1104 32-33 0.43838559432735025 1104 34-35 0.46813634240815816 1104 36-37 0.5402802914112712 1104 38-39 0.4298479913368638 1104 40-41 0.4657341191642743 1104 42-43 0.545417164753502 1104 44-45 0.520099464695754 1104 46-47 0.6636166618820596 1104 48-49 0.5851006240657668 1104 50-51 0.5806718205295311 1104 52-53 0.6857965574456699 1104 54-55 0.6396072911069197 1104 56-57 0.6310502135491234 1104 58-59 0.6456899091547115 1104 60-61 0.6728661285339257 1104 62-63 0.5528274404614777 1104 64-65 0.5801336097235392 1104 66-67 0.6549954767357207 1104 68-69 0.8071957999996826 1104 70-71 0.8195345002001773 1104 72-73 0.7292250015118391 1104 74-75 0.7515783099938318 1104 76-77 0.7580725696218593 1104 78-79 0.650956173147474 1104 80-81 0.743723911247006 1104 82-83 0.8380464304624233 1104 84-85 0.8645127088304569 1104 86-87 0.801277450696567 1104 88-89 0.935261381299501 1104 90-91 0.8616561279425028 1104 92-93 0.8153190534756227 1104 94-95 0.7752726406417132 1104 96-97 0.7019830275728971 1104 98-99 1.021448019483259 1104 100-101 0.7965353878367161 1105 1 0.37922191790813287 1105 2 0.36974103384734747 1105 3 0.2828101406083192 1105 4 0.25720510288474685 1105 5 0.2642752494649869 1105 6 0.29515022026351545 1105 7 0.2015720012639619 1105 8 0.18352559896743514 1105 9 0.34049979300958455 1105 10-11 0.33668259303774306 1105 12-13 0.20843161663987786 1105 14-15 0.3483315160796323 1105 16-17 0.3271273462982478 1105 18-19 0.27619949718955894 1105 20-21 0.4049976072201176 1105 22-23 0.2741802909782578 1105 24-25 0.2654334745693916 1105 26-27 0.29618805138513693 1105 28-29 0.4029875465179842 1105 30-31 0.4270495541325161 1105 32-33 0.3526627930676085 1105 34-35 0.39296618639018277 1105 36-37 0.4604691829158867 1105 38-39 0.47646023043224517 1105 40-41 0.5546503291463907 1105 42-43 0.3926504468147485 1105 44-45 0.3828025652549414 1105 46-47 0.3574043477976261 1105 48-49 0.39421426089231915 1105 50-51 0.328818467090116 1105 52-53 0.3093942364429054 1105 54-55 0.36475479937656985 1105 56-57 0.4131704917291543 1105 58-59 0.35473846401498577 1105 60-61 0.34211187191422 1105 62-63 0.37274784287503593 1105 64-65 0.3420486185228384 1105 66-67 0.4011893179060735 1105 68-69 0.25054054196015585 1105 70-71 0.44709072016974716 1105 72-73 0.5121591853123455 1105 74-75 0.46721341756474644 1105 76-77 0.43894944554337556 1105 78-79 0.5390759500148476 1105 80-81 0.6590718378868665 1105 82-83 0.5966347351347068 1105 84-85 0.6127588419820356 1105 86-87 0.8118342527596809 1105 88-89 0.7977427847199827 1105 90-91 0.5667830682601114 1105 92-93 0.6565340895852323 1105 94-95 0.8249140940161013 1105 96-97 0.7695383718387632 1105 98-99 0.9007538801995381 1105 100-101 0.7775504226438628 1106 1 0.0118681320601155 1106 2 0.06833172140066068 1106 3 0.007602040687594069 1106 4 0.1325687774581894 1106 5 0.007996059404220546 1106 6 0.02143552077039601 1106 7 0.07894145024860677 1106 8 0.0423926540996149 1106 9 0.05338015375045302 1106 10-11 -0.45854804922482373 1106 12-13 -0.23695082252447008 1106 14-15 0.06661511739112314 1106 16-17 0.08795799976197571 1106 18-19 0.07038009640426424 1106 20-21 0.12067165441452232 1106 22-23 -0.03358403072655136 1106 24-25 -0.006503964818179497 1106 26-27 0.046906946823597195 1106 28-29 -0.003937916342366066 1106 30-31 0.15092198133222468 1106 32-33 0.07367859331282034 1106 34-35 -0.22752280252251467 1106 36-37 -0.3037314621549143 1106 38-39 -0.08989556871927817 1106 40-41 0.07753848050791845 1106 42-43 0.08022443327455164 1106 44-45 0.12132216504581805 1106 46-47 0.21766765175520675 1106 48-49 0.2302909106665325 1106 50-51 0.37820991896433753 1106 52-53 0.2865346823918671 1106 54-55 0.04279802213099515 1106 56-57 0.10722028301501751 1106 58-59 0.3641868701078934 1106 60-61 0.10724651967592536 1106 62-63 0.20852458413423136 1106 64-65 0.24827453701165325 1106 66-67 -0.0609395874100116 1106 68-69 -0.21200886930510165 1106 70-71 0.005733699193186226 1106 72-73 0.2924086014120668 1106 74-75 0.36151676726745663 1106 76-77 0.35850996630238186 1106 78-79 0.5210378496793666 1106 80-81 0.24831149550110965 1106 82-83 0.3961916287244449 1106 84-85 0.41082108029683084 1106 86-87 0.5173372338806992 1106 88-89 0.2826811822825661 1106 90-91 0.4108241680770064 1106 92-93 0.5184088817098633 1106 94-95 0.45394641386427637 1106 96-97 0.6591072485601828 1106 98-99 0.5378675490270943 1106 100-101 0.4726846662126363 1107 1 0.3160664048887014 1107 2 0.32458407137703915 1107 3 0.249962121492878 1107 4 0.21171899585999654 1107 5 0.2773944629398457 1107 6 0.2347964221365615 1107 7 0.09240673640383079 1107 8 0.1704620654633473 1107 9 0.200343689149733 1107 10-11 0.20495574075177103 1107 12-13 0.15486185349614345 1107 14-15 0.25255994164114526 1107 16-17 0.32002207584734066 1107 18-19 0.43477782555788735 1107 20-21 0.4268618030121516 1107 22-23 0.4026526489731381 1107 24-25 0.37533137395391947 1107 26-27 0.37235628993534675 1107 28-29 0.3880015987506056 1107 30-31 0.44555972768572616 1107 32-33 0.4763811571951351 1107 34-35 0.5375202486679811 1107 36-37 0.48474862398514773 1107 38-39 0.5156801262018647 1107 40-41 0.5407069255588439 1107 42-43 0.5581405577355767 1107 44-45 0.5069678876932286 1107 46-47 0.5417561120871071 1107 48-49 0.6147287109203674 1107 50-51 0.5598199865205871 1107 52-53 0.4857134056774939 1107 54-55 0.6089657591436222 1107 56-57 0.5811437395279242 1107 58-59 0.5805812974269671 1107 60-61 0.549218711527935 1107 62-63 0.6797029166048105 1107 64-65 0.7604443064634339 1107 66-67 0.6676312652725613 1107 68-69 0.6341153681848581 1107 70-71 0.7085001472696675 1107 72-73 0.6051665022932333 1107 74-75 0.5488636656000345 1107 76-77 0.8196014529391924 1107 78-79 0.746166528546194 1107 80-81 0.7928470170972837 1107 82-83 0.7284404251974657 1107 84-85 0.7274822966508872 1107 86-87 0.7924776171054937 1107 88-89 0.9292407163988834 1107 90-91 1.0398566773080056 1107 92-93 0.8813369729978291 1107 94-95 0.9447874397989686 1107 96-97 0.9580155157802217 1107 98-99 1.1521707140010236 1107 100-101 1.1655297243740002 1108 1 0.17951267244111335 1108 2 0.143779135856839 1108 3 0.06295325549784536 1108 4 0.049703898238377064 1108 5 0.03661316383843172 1108 6 0.12577586837965526 1108 7 0.05841448724044085 1108 8 0.07095506835680965 1108 9 0.09740537080287481 1108 10-11 0.05161174621138542 1108 12-13 -0.10892287021229663 1108 14-15 -0.03217323863216848 1108 16-17 0.04706657991237506 1108 18-19 0.09306575704032838 1108 20-21 0.13992660149771297 1108 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-0.8205757724394616 2316 64-65 -0.7563382831502494 2316 66-67 -0.990974501605514 2316 68-69 -0.889032750040851 2316 70-71 -0.9516146338370959 2316 72-73 -0.8969295901816672 2316 74-75 -0.850561751764122 2316 76-77 -0.9184857830410245 2316 78-79 -0.8959191796456025 2316 80-81 -0.8310880475859683 2316 82-83 -0.9937058282276716 2316 84-85 -0.9499704516293512 2316 86-87 -0.9666405621301912 2316 88-89 -1.0771828275470376 2316 90-91 -1.0301159708473087 2316 92-93 -1.2231741885676435 2316 94-95 -1.4172183949739754 2316 96-97 -1.5308853967994445 2316 98-99 -1.4930702896981067 2316 100-101 -1.5362071346912067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8175.0 3 2312.0 4 2219.0 5 2592.0 6 2835.0 7 3521.0 8 4328.0 9 5452.0 10 6619.0 11 8218.0 12 10211.0 13 12477.0 14 14549.0 15 17293.0 16 21122.0 17 27515.0 18 25160.0 19 19982.0 20 19935.0 21 20707.0 22 22495.0 23 24181.0 24 26884.0 25 29965.0 26 33836.0 27 39494.0 28 45848.0 29 54655.0 30 65268.0 31 80159.0 32 100360.0 33 130740.0 34 178142.0 35 260778.0 36 394560.0 37 552394.0 38 424061.0 39 50626.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47295186398853 18.44307381030524 14.762280336914802 23.321693988791438 2 15.504805942951624 19.39908247533716 40.599520278983384 24.49659130272783 3 19.340733718939397 23.46929009790561 29.44820973379354 27.74176644936146 4 12.911910649656914 16.367394694368475 36.65591274585264 34.064781910121965 5 14.064311361770972 36.793240191234304 34.0951767388618 15.047271708132918 6 32.77336899357511 35.59923441645371 17.07308104416159 14.554315545809587 7 29.091971784750637 32.48262022451955 20.04375241562684 18.381655575102975 8 26.437789978615584 34.9674685428578 19.238297062544596 19.356444415982022 9 26.24973028320173 17.125497543348448 20.09156362241759 36.53320855103224 10-11 25.26161606253927 26.34573155230292 26.814543168902294 21.57810921625551 12-13 26.103456166209664 23.61701744220655 27.403216114467565 22.876310277116225 14-15 22.918109697842688 25.148233074847674 25.75806782924263 26.175589398067007 16-17 22.444013420767252 28.36643643694641 26.496462856352665 22.693087285933665 18-19 22.322733412712044 26.803761124421765 28.834304703625318 22.039200759240877 20-21 22.802120060963126 25.95085644093628 29.260844839744323 21.98617865835627 22-23 22.62314280239889 25.780094411771504 29.154870350762497 22.441892435067114 24-25 22.431339959409232 26.07486268173887 29.19641311724966 22.29738424160224 26-27 22.249409752391696 26.36246434631499 29.01965672381871 22.368469177474605 28-29 22.0907467951045 26.574407018589675 29.082460313084503 22.25238587322132 30-31 22.337434801684115 26.21209317188044 28.99044264522599 22.460029381209456 32-33 22.11863788384421 26.249503899743804 29.016656048619915 22.61520216779207 34-35 22.62334096542919 26.392347959515316 28.749690184950033 22.234620890105464 36-37 22.704802304864682 26.07573787095531 28.72049839987466 22.498961424305346 38-39 22.253000486626856 26.36855389960933 28.732887334512757 22.64555827925105 40-41 22.705093945469585 26.216099048565734 28.724531591587123 22.354275414377554 42-43 22.482884559164496 26.23126297427094 28.589412612878775 22.69643985368579 44-45 22.39991677274686 26.48987422972504 28.606076280614793 22.504132716913304 46-47 22.683505816756924 26.769741404916093 28.011669346212127 22.53508343211486 48-49 22.67343565637052 26.637245292085932 28.03361770680981 22.655701344733735 50-51 22.61003686842139 26.86498373024029 27.894389342898148 22.630590058440177 52-53 22.77513536912984 26.82635100847046 27.818397105819827 22.580116516579878 54-55 22.62684120665185 26.760249307151728 27.893470247220918 22.719439238975493 56-57 22.592704148837374 26.996617299390646 27.805297005769763 22.605381546002217 58-59 22.72003887858844 26.706130907495663 27.76965382123634 22.80417639267956 60-61 22.484006178529828 26.826438752740213 27.987768810065504 22.70178625866446 62-63 22.442961344938247 26.816702420499634 28.1474361364399 22.592900098122218 64-65 22.36106351912034 26.77566020700879 28.065463783646933 22.797812490223937 66-67 22.330628215214933 26.844214368152862 27.84820348075263 22.97695393587957 68-69 22.50718307968699 26.582450915167456 28.05907894349165 22.851287061653903 70-71 22.49219480230774 26.612003904690905 27.868949084387822 23.026852208613533 72-73 22.53780822874706 26.692329353621908 27.746062734300413 23.02379968333062 74-75 22.43610662120615 27.02010252626731 27.62264716550269 22.921143687023854 76-77 22.607313189765275 26.802952165361422 27.564874630460967 23.02486001441234 78-79 22.638643822676453 26.932585785905065 27.61711618811919 22.81165420329929 80-81 22.693971961155047 27.07977925102194 27.406876071680852 22.819372716142162 82-83 22.685104855193217 27.063719932180874 27.46501825885561 22.786156953770302 84-85 22.757184267088903 27.03342503383752 27.336389531810905 22.873001167262665 86-87 22.766183413228973 26.921332499218014 27.373173251085685 22.939310836467328 88-89 22.76612891489114 27.030376790080336 27.250844102922343 22.952650192106184 90-91 22.87029080576943 26.973396340006794 27.215593478507277 22.940719375716498 92-93 22.835241222409643 26.679760040906338 27.380013631806964 23.104985104877056 94-95 22.793051557672044 26.641232637672797 27.387478619750595 23.178237184904564 96-97 22.906180034161203 26.478079652108043 27.377462738401192 23.238277575329562 98-99 22.80535519290689 26.430800926912084 27.148304660045223 23.615539220135798 100-101 23.245630572269214 25.980355994995286 27.30825619792711 23.46575723480839 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 856.0 1 571.5 2 419.0 3 546.0 4 442.0 5 422.0 6 829.0 7 1925.0 8 3252.5 9 3853.0 10 3325.5 11 2660.0 12 2566.5 13 2688.0 14 2920.5 15 3063.0 16 3225.0 17 3483.5 18 3743.5 19 4059.0 20 4520.5 21 5287.0 22 6361.5 23 7755.5 24 9640.5 25 12128.0 26 15313.5 27 19099.0 28 22396.0 29 25777.0 30 30256.5 31 35586.0 32 41797.0 33 46145.0 34 49654.5 35 54150.5 36 59858.0 37 65356.5 38 69291.0 39 72934.5 40 76231.5 41 80395.5 42 84266.0 43 87800.5 44 91556.5 45 95470.0 46 97772.5 47 100392.0 48 102646.5 49 102905.0 50 102317.5 51 99469.0 52 97085.0 53 96849.0 54 98980.0 55 94564.5 56 84910.5 57 77747.5 58 72273.0 59 64381.5 60 52762.5 61 43749.0 62 36366.0 63 28038.0 64 21592.5 65 16941.5 66 12312.0 67 9360.5 68 6873.0 69 4601.5 70 3389.5 71 2358.0 72 1659.5 73 1260.0 74 901.0 75 620.5 76 419.0 77 241.0 78 143.0 79 97.5 80 64.0 81 46.5 82 28.5 83 18.5 84 14.0 85 11.5 86 7.0 87 7.5 88 8.5 89 7.5 90 6.5 91 4.5 92 3.5 93 3.0 94 2.5 95 1.0 96 0.5 97 1.0 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49274985989232895 2 0.051169758127426884 3 0.058770667472581264 4 0.06677162467800693 5 0.08204617934291049 6 0.10939490579054731 7 0.07044479139504327 8 0.05120612611472427 9 0.05156980598769816 10-11 0.0758454375087056 12-13 0.1080674742541926 14-15 0.05635219631730487 16-17 0.06149826651988547 18-19 0.06047996287555856 20-21 0.07668190121654554 22-23 0.06598971295111306 24-25 0.09071994431333785 26-27 0.06849910407463293 28-29 0.06846273608733554 30-31 0.07260868663923793 32-33 0.0723904787154536 34-35 0.07506352578181172 36-37 0.06973561564274416 38-39 0.04213231328402562 40-41 0.045150856229708936 42-43 0.04373250472511075 44-45 0.020784304740458042 46-47 0.027621486352367247 48-49 0.027730590314259417 50-51 0.025675799031956912 52-53 0.025675799031956912 54-55 0.025184831203442157 56-57 0.024875703311414348 58-59 0.021820792378433637 60-61 0.02898528587601935 62-63 0.019947841032618083 64-65 0.022148104264110142 66-67 0.015929178436256554 68-69 0.01825672962328948 70-71 0.042986960985514265 72-73 0.03865917049712493 74-75 0.033167604415219126 76-77 0.023202775895734433 78-79 0.01505634674111921 80-81 0.017638473839233858 82-83 0.021602584454649302 84-85 0.018329465597884256 86-87 0.00912836481164474 88-89 0.04331427287119077 90-91 0.054424692990543234 92-93 0.0346405079007634 94-95 0.055115684749193636 96-97 0.04478717635673504 98-99 0.035440603621305966 100-101 0.1694930047994833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2749671.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.02297212712371 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53062293328382 42.874729554627734 2 13.36238116438012 14.972006150481404 3 4.378687069968381 7.359211910227067 4 1.964854369919033 4.403079263993249 5 1.0201826167745873 2.8576831152069415 6 0.6014164524958631 2.021588229298161 7 0.4058882187098488 1.5917345054456828 8 0.2855883981043695 1.27996086934648 9 0.1977359640557506 0.9969980762542727 >10 1.1267196673321342 11.55102542142134 >50 0.08157068180241828 3.162757357696646 >100 0.04005366721117755 3.945763287271707 >500 0.0031263970636391156 1.2320711720301227 >1k 9.769990823526701E-4 0.8074782456729426 >5k 1.3026654431368937E-4 0.5324196792721725 >10k+ 6.513327215684468E-5 0.41149316175397777 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11277 0.41012179275266025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8909 0.32400239883244214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5682 0.20664290382376654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 3.63679872973894E-5 9 0.0 0.0 0.0 0.0 3.63679872973894E-5 10-11 0.0 0.0 0.0 0.0 3.63679872973894E-5 12-13 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 14-15 0.0 0.0 0.0 3.63679872973894E-5 5.45519809460841E-5 16-17 0.0 0.0 0.0 3.63679872973894E-5 7.27359745947788E-5 18-19 0.0 0.0 0.0 7.27359745947788E-5 7.27359745947788E-5 20-21 0.0 0.0 0.0 1.454719491895576E-4 7.27359745947788E-5 22-23 0.0 0.0 0.0 3.091278920278099E-4 9.091996824347349E-5 24-25 0.0 0.0 0.0 5.091518221634516E-4 1.0910396189216819E-4 26-27 0.0 0.0 0.0 0.001018303644326903 1.0910396189216819E-4 28-29 0.0 0.0 0.0 0.0033458548313598244 1.0910396189216819E-4 30-31 0.0 0.0 0.0 0.011219524081244629 1.0910396189216819E-4 32-33 0.0 0.0 0.0 0.028348846098315034 1.8183993648694698E-4 34-35 0.0 0.0 0.0 0.05458834893338149 1.8183993648694698E-4 36-37 0.0 0.0 0.0 0.09697523812848882 1.8183993648694698E-4 38-39 0.0 0.0 0.0 0.1618375434733828 1.8183993648694698E-4 40-41 0.0 0.0 0.0 0.2284818801958489 1.8183993648694698E-4 42-43 0.0 0.0 0.0 0.29387152135655503 1.8183993648694698E-4 44-45 0.0 0.0 0.0 0.3745902691631108 1.8183993648694698E-4 46-47 0.0 0.0 0.0 0.46798326054280676 2.1820792378433637E-4 48-49 0.0 0.0 0.0 0.5665586901123807 2.1820792378433637E-4 50-51 0.0 0.0 0.0 0.6702256379035891 2.1820792378433637E-4 52-53 0.0 0.0 0.0 0.7660189164449128 2.363919174330311E-4 54-55 0.0 0.0 0.0 0.872449831270723 2.727599047304205E-4 56-57 0.0 0.0 0.0 0.996628323897659 2.909438983791152E-4 58-59 0.0 0.0 0.0 1.128025862003127 2.909438983791152E-4 60-61 0.0 0.0 0.0 1.268279005015509 2.909438983791152E-4 62-63 0.0 0.0 0.0 1.40394978162842 2.909438983791152E-4 64-65 0.0 0.0 0.0 1.5439483487297208 2.909438983791152E-4 66-67 0.0 0.0 0.0 1.7073497156568913 2.909438983791152E-4 68-69 0.0 0.0 0.0 1.8890623641882973 2.909438983791152E-4 70-71 0.0 0.0 0.0 2.0770303065348545 3.2731188567650457E-4 72-73 0.0 0.0 0.0 2.2639981292307336 3.6367987297389396E-4 74-75 0.0 0.0 0.0 2.450693192021882 3.6367987297389396E-4 76-77 0.0 0.0 0.0 2.655535880474428 3.6367987297389396E-4 78-79 0.0 0.0 0.0 2.8834176888798697 3.6367987297389396E-4 80-81 0.0 0.0 0.0 3.1181003109099237 3.6367987297389396E-4 82-83 0.0 0.0 0.0 3.36040202627878 4.5459984121736744E-4 84-85 0.0 0.0 0.0 3.604976740853724 4.909678285147568E-4 86-87 0.0 0.0 0.0 3.8610255554209942 5.091518221634516E-4 88-89 0.0 0.0 0.0 4.1350583397068235 5.091518221634516E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5560 0.0 48.10149 1 GTATCAA 9765 0.0 42.304752 1 ATCAACG 12225 0.0 33.19454 3 TATCAAC 12325 0.0 33.002316 2 TCAACGC 12370 0.0 32.882263 4 CAACGCA 12545 0.0 32.385685 5 AACGCAG 13060 0.0 31.181376 6 ACGCAGA 14810 0.0 27.3489 7 CGCAGAG 14950 0.0 26.997395 8 GCAGAGT 16310 0.0 24.454311 9 TGGTATC 2950 0.0 23.195198 2 GTACATG 8995 0.0 22.777256 1 GAGTACT 9650 0.0 22.536142 12-13 TACATGG 8735 0.0 22.194986 2 GTGGTAT 3430 0.0 21.02462 1 GTACTTT 10375 0.0 20.947594 14-15 CAGAGTA 15505 0.0 20.925617 10-11 AGAGTAC 14525 0.0 20.897396 10-11 AGTACTT 10085 0.0 20.71566 12-13 ACATGGG 8845 0.0 20.414719 3 >>END_MODULE