FastQCFastQC Report
Thu 26 May 2016
SRR938177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749671
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110850.4031391391915615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91250.3318578840886782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.2106433824264794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.049.0206571
GTATCAA95100.038.4108541
ATCAACG117950.030.7269153
TCAACGC119700.030.276594
TATCAAC120650.030.2377832
CAACGCA121800.029.7935755
AACGCAG127500.028.573396
ACGCAGA145650.024.8823057
CGCAGAG146550.024.5350328
GTACATG90800.022.076731
GCAGAGT162900.021.8971469
GAGTACT96750.021.6249812-13
TACATGG90450.021.164722
ACATGGG89550.020.7927933
TGGTATC29750.020.7573762
GTACTTT101000.020.59745214-15
TAAGGTG13700.020.45545
GTAAGGT11000.020.2946594
AGAGTAC141050.020.28825810-11
CAGAGTA153700.019.94725410-11
Produced by FastQC (version 0.11.5)