##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938177_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2749671 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51336287141262 34.0 31.0 34.0 31.0 34.0 2 32.72974803167361 34.0 31.0 34.0 31.0 34.0 3 32.79006106548747 34.0 31.0 34.0 31.0 34.0 4 36.24347130984034 37.0 35.0 37.0 35.0 37.0 5 36.20223474008345 37.0 37.0 37.0 35.0 37.0 6 36.23983014695212 37.0 37.0 37.0 35.0 37.0 7 36.2072386114557 37.0 37.0 37.0 35.0 37.0 8 36.2003097825158 37.0 37.0 37.0 35.0 37.0 9 37.912541536787494 39.0 38.0 39.0 35.0 39.0 10-11 37.899533616930896 39.0 38.0 39.0 35.0 39.0 12-13 37.84900557193933 39.0 38.0 39.0 35.0 39.0 14-15 39.183513227582495 40.5 39.0 41.0 36.0 41.0 16-17 39.13605227679966 40.0 38.5 41.0 36.0 41.0 18-19 39.160667585322024 40.0 39.0 41.0 36.0 41.0 20-21 39.089246677147926 40.0 39.0 41.0 36.0 41.0 22-23 38.94676617675351 40.0 38.0 41.0 35.0 41.0 24-25 38.91069695247177 40.0 38.0 41.0 35.0 41.0 26-27 38.88246539313249 40.0 38.0 41.0 35.0 41.0 28-29 38.82877551532529 40.0 38.0 41.0 35.0 41.0 30-31 38.644916246343655 40.0 38.0 41.0 34.5 41.0 32-33 38.48037874349331 40.0 38.0 41.0 34.0 41.0 34-35 38.28838959279128 40.0 38.0 41.0 34.0 41.0 36-37 38.162251411168825 40.0 38.0 41.0 34.0 41.0 38-39 38.16564381702393 40.0 38.0 41.0 34.0 41.0 40-41 38.1095745636478 40.0 38.0 41.0 34.0 41.0 42-43 37.9636947474807 40.0 38.0 41.0 33.0 41.0 44-45 37.771599038575886 40.0 37.5 41.0 33.0 41.0 46-47 37.46162995500188 40.0 37.0 41.0 32.5 41.0 48-49 37.13451409277692 40.0 37.0 41.0 31.5 41.0 50-51 36.85242198066605 40.0 36.0 41.0 31.0 41.0 52-53 36.44436879903087 39.0 36.0 41.0 30.0 41.0 54-55 36.229371804845016 39.0 35.0 41.0 30.0 41.0 56-57 35.990152640079486 39.0 35.0 40.5 29.0 41.0 58-59 35.66076759728709 38.5 35.0 40.0 28.5 41.0 60-61 35.31171601984383 38.0 35.0 40.0 28.0 41.0 62-63 34.95021658954835 37.5 34.0 40.0 27.5 41.0 64-65 34.5649839562624 37.0 34.0 40.0 26.5 41.0 66-67 34.1461818523016 36.5 34.0 39.0 26.0 41.0 68-69 33.68790775332758 36.0 33.0 39.0 26.0 40.5 70-71 33.181490076449144 35.5 33.0 38.5 25.0 40.0 72-73 32.7638021057792 35.0 32.5 37.5 24.0 40.0 74-75 32.3169826499243 35.0 32.0 37.0 23.5 39.0 76-77 31.00705866265455 34.0 30.5 35.5 21.5 38.5 78-79 31.72568463645287 35.0 32.0 36.0 23.0 39.0 80-81 31.566573964667043 35.0 32.0 36.0 22.5 37.5 82-83 31.199445497297674 35.0 31.5 35.0 20.5 37.0 84-85 30.821632115260336 34.0 31.0 35.0 19.5 36.5 86-87 30.393180311389983 34.0 31.0 35.0 17.5 36.0 88-89 29.934783288618895 34.0 30.0 35.0 13.0 36.0 90-91 29.599770481632163 34.0 30.0 35.0 7.0 35.0 92-93 29.194407803697246 34.0 29.5 35.0 2.0 35.0 94-95 28.77885481572159 34.0 29.0 35.0 2.0 35.0 96-97 28.20273043575031 34.0 29.0 35.0 2.0 35.0 98-99 27.52140128764496 33.5 28.0 35.0 2.0 35.0 100-101 25.074679843515824 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15417961686085135 1101 2 0.14874867657776747 1101 3 0.13626427938089591 1101 4 0.1017477246100853 1101 5 0.07534459599309429 1101 6 0.12725223700891064 1101 7 0.13188033984547332 1101 8 0.11604215221709069 1101 9 0.06488223667985693 1101 10-11 0.11382480954129193 1101 12-13 0.1681991093081905 1101 14-15 0.17706240474981172 1101 16-17 0.2136921694071674 1101 18-19 0.27053930272877125 1101 20-21 0.2687638416376714 1101 22-23 0.3634095685166656 1101 24-25 0.25697058504101733 1101 26-27 0.34522116259352487 1101 28-29 0.26806397528324055 1101 30-31 0.2674386989097499 1101 32-33 0.3205954879255586 1101 34-35 0.250092437916976 1101 36-37 0.28492462338671487 1101 38-39 0.3646514981441342 1101 40-41 0.4278121987511767 1101 42-43 0.5340108806318469 1101 44-45 0.5191545572610323 1101 46-47 0.4784452666801329 1101 48-49 0.4883271347584639 1101 50-51 0.44025346784120956 1101 52-53 0.48357315644398824 1101 54-55 0.4909796724685691 1101 56-57 0.525454394883397 1101 58-59 0.4390356647430096 1101 60-61 0.41395323555693864 1101 62-63 0.5788771324301862 1101 64-65 0.558103770352794 1101 66-67 0.4475813763404517 1101 68-69 0.3915616759026008 1101 70-71 0.5095164867284083 1101 72-73 0.4294918413923554 1101 74-75 0.5224665221161473 1101 76-77 0.3616438745596966 1101 78-79 0.43573960635673004 1101 80-81 0.540566649204969 1101 82-83 0.49682700996970297 1101 84-85 0.5258892675265479 1101 86-87 0.6067573824552 1101 88-89 0.5492319880840135 1101 90-91 0.44052085738787383 1101 92-93 0.547220672420778 1101 94-95 0.5348271178614397 1101 96-97 0.6601623237525445 1101 98-99 0.6009729464103977 1101 100-101 0.6981187398117292 1102 1 0.21059264500867414 1102 2 0.158187669558977 1102 3 0.1757682921159187 1102 4 0.07642973537317488 1102 5 0.07991195671448281 1102 6 0.04442676262640788 1102 7 0.046173080298068214 1102 8 0.08378279385360798 1102 9 0.061898534522079274 1102 10-11 0.10347965942577986 1102 12-13 0.1900325236819711 1102 14-15 0.23526498022510367 1102 16-17 0.20050978171558143 1102 18-19 0.1654530201313591 1102 20-21 0.14707626719305011 1102 22-23 0.18091404217950213 1102 24-25 0.20657920735012425 1102 26-27 0.07510661118116957 1102 28-29 0.07491746566080337 1102 30-31 0.09815835452237565 1102 32-33 0.057976191619658834 1102 34-35 0.1729705343442518 1102 36-37 0.22105414804914147 1102 38-39 0.04617158023005885 1102 40-41 0.19829948690766486 1102 42-43 0.13876017962375897 1102 44-45 0.10881331944299433 1102 46-47 0.059032758966502286 1102 48-49 0.12688065646727864 1102 50-51 0.1784425212328884 1102 52-53 0.09355441358024308 1102 54-55 0.07837595959964005 1102 56-57 0.17346984089681428 1102 58-59 0.209796567148949 1102 60-61 0.20144348358015662 1102 62-63 0.19938082593488815 1102 64-65 0.2886538985545073 1102 66-67 0.2624521176484933 1102 68-69 0.4142654120809297 1102 70-71 0.4191286892828501 1102 72-73 0.3083050574619719 1102 74-75 0.2146693132609414 1102 76-77 0.2933995985960962 1102 78-79 0.2764758119739348 1102 80-81 0.332089686883684 1102 82-83 0.4253245956876519 1102 84-85 0.5225577325349917 1102 86-87 0.5322210472390303 1102 88-89 0.4430090022514612 1102 90-91 0.41556968713422293 1102 92-93 0.40579761202443976 1102 94-95 0.5095651829706185 1102 96-97 0.536983226993538 1102 98-99 0.5024476524209689 1102 100-101 0.6564017310820454 1103 1 0.18925040948433036 1103 2 0.17673262178644222 1103 3 0.10920906568561861 1103 4 0.07851330852847127 1103 5 0.12854784942773279 1103 6 0.10970821767418215 1103 7 0.06495977305736744 1103 8 0.09773467597631935 1103 9 0.1521598445255279 1103 10-11 0.12369952819520336 1103 12-13 0.16498360000291257 1103 14-15 0.2341195905623934 1103 16-17 0.3124644265872334 1103 18-19 0.20659495635790392 1103 20-21 0.2804364605854843 1103 22-23 0.2908484268922962 1103 24-25 0.2874241480661297 1103 26-27 0.304552910248745 1103 28-29 0.27574629034135967 1103 30-31 0.24057806213144062 1103 32-33 0.3499699754345542 1103 34-35 0.3100719502530822 1103 36-37 0.388816321411646 1103 38-39 0.27935910181403045 1103 40-41 0.34384455424967797 1103 42-43 0.34273163121809347 1103 44-45 0.3538864171752323 1103 46-47 0.279195070466443 1103 48-49 0.33706829316578535 1103 50-51 0.45124095567516065 1103 52-53 0.36906955114211115 1103 54-55 0.43523103039507305 1103 56-57 0.4693775190365699 1103 58-59 0.5062280164409927 1103 60-61 0.5833589895085609 1103 62-63 0.5892852808675499 1103 64-65 0.47453510035028756 1103 66-67 0.49250852377258525 1103 68-69 0.4809685316849368 1103 70-71 0.537713931598951 1103 72-73 0.41999493889398565 1103 74-75 0.3019491567051702 1103 76-77 0.43892739875725795 1103 78-79 0.43517847111978725 1103 80-81 0.41875942327138205 1103 82-83 0.517617677303857 1103 84-85 0.5822561324429003 1103 86-87 0.5839189866887828 1103 88-89 0.5576515716079733 1103 90-91 0.6162661300949637 1103 92-93 0.7460243876662034 1103 94-95 0.5143194134299236 1103 96-97 0.6041813391150974 1103 98-99 0.8113114719213748 1103 100-101 0.7902741841728691 1104 1 0.110051353100026 1104 2 0.08916767202565268 1104 3 0.08614250792818012 1104 4 0.031243781235630763 1104 5 0.07501609121513297 1104 6 0.018652276336510454 1104 7 0.05783226379103468 1104 8 0.02333144080745342 1104 9 0.04417961764452372 1104 10-11 0.04737108215098118 1104 12-13 0.0995502881987207 1104 14-15 0.1361364427537879 1104 16-17 0.07359929242895191 1104 18-19 0.11286320751011658 1104 20-21 0.19254515476733758 1104 22-23 0.20164010232298324 1104 24-25 0.17162705814244106 1104 26-27 0.17921739377597135 1104 28-29 0.14770679761655003 1104 30-31 0.11923299355652972 1104 32-33 0.027523204323046002 1104 34-35 0.08324222695337369 1104 36-37 0.12337914476687217 1104 38-39 0.09153580232530345 1104 40-41 0.0395895134198625 1104 42-43 0.11091656086684765 1104 44-45 0.09014575689695192 1104 46-47 0.082310652240146 1104 48-49 -0.00391217229479679 1104 50-51 0.16257697903663626 1104 52-53 0.037751187129131836 1104 54-55 0.026864170808451604 1104 56-57 0.073457155563176 1104 58-59 0.23458591257043082 1104 60-61 0.18696139254751643 1104 62-63 0.12266271302286924 1104 64-65 0.16956164511591965 1104 66-67 0.18020785106735104 1104 68-69 0.3195944916704576 1104 70-71 0.30531133115582776 1104 72-73 0.024881881158478336 1104 74-75 -0.030605361702242817 1104 76-77 0.058303182409428445 1104 78-79 0.04891508074784312 1104 80-81 0.050011936346813 1104 82-83 0.1717549519817929 1104 84-85 0.35532540302113347 1104 86-87 0.20498519215114186 1104 88-89 0.19588829755287307 1104 90-91 0.253728624418482 1104 92-93 0.4017691387295912 1104 94-95 0.2973337363804589 1104 96-97 0.22973886094959184 1104 98-99 0.3747960121874385 1104 100-101 0.4815122189608978 1105 1 0.1661127399292468 1105 2 0.1392866056125328 1105 3 0.06627802861785881 1105 4 0.08518463493312112 1105 5 0.10050017730955574 1105 6 0.08045839027249002 1105 7 0.04018586435348226 1105 8 0.07876306920204001 1105 9 0.02349624452810417 1105 10-11 0.07393788164656456 1105 12-13 0.1477421886877437 1105 14-15 0.25715502194393736 1105 16-17 0.1580741466388318 1105 18-19 0.09913371155102624 1105 20-21 0.1644778813248351 1105 22-23 0.16781777900070693 1105 24-25 0.1398164285128658 1105 26-27 0.13400143097231876 1105 28-29 0.13555412255652044 1105 30-31 0.1338918854892981 1105 32-33 0.18802535111343843 1105 34-35 0.2669652091733994 1105 36-37 0.2888590158444444 1105 38-39 0.2680717789905742 1105 40-41 0.3242220358635777 1105 42-43 0.213765743213159 1105 44-45 0.20008786207478124 1105 46-47 0.11727222173396257 1105 48-49 0.16084545107307235 1105 50-51 0.06910586277907527 1105 52-53 0.04259693233842654 1105 54-55 0.054484863077163936 1105 56-57 0.021795002049820766 1105 58-59 0.14638620002848057 1105 60-61 0.21133976416263778 1105 62-63 0.19567166834023197 1105 64-65 0.27602954828913795 1105 66-67 0.17116544830268765 1105 68-69 0.14093728906990322 1105 70-71 0.2309646414474429 1105 72-73 0.10334146847202419 1105 74-75 0.19607545940299076 1105 76-77 0.11033619366762437 1105 78-79 0.11936232562743854 1105 80-81 0.19019948935494568 1105 82-83 0.35064174968163186 1105 84-85 0.4970326899151054 1105 86-87 0.3957587669052707 1105 88-89 0.1756965378415316 1105 90-91 0.20883765576409985 1105 92-93 0.24181070417188621 1105 94-95 0.17251485255073362 1105 96-97 0.39131353692883053 1105 98-99 0.4676917550896995 1105 100-101 0.6265052652915806 1106 1 -0.1591822614722176 1106 2 -0.02345031947255194 1106 3 -0.024287606370563708 1106 4 -0.06977368960527741 1106 5 -0.08574069895959013 1106 6 -0.034985848142163434 1106 7 -0.06457156572972877 1106 8 -0.1316052192469499 1106 9 -0.18241548660381568 1106 10-11 -0.10984950388824899 1106 12-13 -0.22431384037822255 1106 14-15 -0.36123751332585385 1106 16-17 -0.22368971169709084 1106 18-19 -0.1493330631293759 1106 20-21 -0.10656471981783966 1106 22-23 -0.12060927910084018 1106 24-25 -0.1287621147599438 1106 26-27 -0.18337502138139428 1106 28-29 -0.1513805257863794 1106 30-31 -0.190911215753637 1106 32-33 -0.10917559961174561 1106 34-35 -0.17684867081194255 1106 36-37 -0.27018676917617057 1106 38-39 -0.14031551498000283 1106 40-41 -0.08669133185345146 1106 42-43 -0.1885231247586674 1106 44-45 -0.11045257822076593 1106 46-47 -0.15634314668525917 1106 48-49 -0.1685339463412987 1106 50-51 -0.2588646712918816 1106 52-53 -0.3403651731210573 1106 54-55 -0.6797548486936478 1106 56-57 -0.4442389221537013 1106 58-59 -0.4441441011005409 1106 60-61 -0.45090862903836637 1106 62-63 -0.39058700034335203 1106 64-65 -0.5086593856216197 1106 66-67 -0.5591242021655347 1106 68-69 -0.676901343373693 1106 70-71 -0.7523031580337616 1106 72-73 -0.5024686464429351 1106 74-75 -0.6148963181556368 1106 76-77 -0.4496241769323852 1106 78-79 -0.45423658447820614 1106 80-81 -0.6381173825827346 1106 82-83 -0.7281602198210884 1106 84-85 -0.4971260617469113 1106 86-87 -0.31432302008188273 1106 88-89 -0.3021290547568256 1106 90-91 -0.4938927071298238 1106 92-93 -0.3303661997155274 1106 94-95 -0.507477874618246 1106 96-97 -0.4253553136125099 1106 98-99 -0.17240739370111768 1106 100-101 -0.013601874895474708 1107 1 0.07322007306997591 1107 2 0.08137614583897346 1107 3 0.05390328166117797 1107 4 0.06912539245659133 1107 5 0.07508986337955292 1107 6 0.06784434598692002 1107 7 0.035638962013749165 1107 8 0.09720807188037384 1107 9 0.06983678601328336 1107 10-11 0.07898970008480433 1107 12-13 0.04999574692259756 1107 14-15 0.10861553921339606 1107 16-17 0.038410233473264555 1107 18-19 0.10312817999901824 1107 20-21 0.11383631518899051 1107 22-23 0.09538585630678398 1107 24-25 0.0633735864704974 1107 26-27 0.022133599531279913 1107 28-29 0.12628935505772887 1107 30-31 0.10396149684183342 1107 32-33 0.119984426841107 1107 34-35 0.06929447951396384 1107 36-37 0.1526088619336221 1107 38-39 0.058912499727306056 1107 40-41 0.0032540218555112688 1107 42-43 0.1806850704017009 1107 44-45 0.19989456399446226 1107 46-47 0.16772327378802032 1107 48-49 0.12334454785536764 1107 50-51 0.048552613935029854 1107 52-53 0.10394556703391089 1107 54-55 0.10538666980363587 1107 56-57 0.08192797847453903 1107 58-59 0.06274570940188795 1107 60-61 0.08069759567073476 1107 62-63 0.1412724001775203 1107 64-65 0.16371039272144827 1107 66-67 0.10968811694750968 1107 68-69 0.20446927461892273 1107 70-71 0.11258491088029388 1107 72-73 0.035089972673894465 1107 74-75 0.10828042041245567 1107 76-77 0.1261068493865949 1107 78-79 0.2681278816938182 1107 80-81 0.29484923513158634 1107 82-83 0.2706333647842136 1107 84-85 0.43188826303094174 1107 86-87 0.42797690635145713 1107 88-89 0.5993440492062554 1107 90-91 0.567860841789745 1107 92-93 0.6443003328282408 1107 94-95 0.6572303842128306 1107 96-97 0.8202600731474483 1107 98-99 0.8133560506754272 1107 100-101 1.0044419250222951 1108 1 0.03693971689549613 1108 2 0.0024889686741005335 1108 3 -0.01955521654498682 1108 4 0.008230021317217506 1108 5 -0.02335197219446883 1108 6 -0.04039592059601205 1108 7 -0.051294117330598965 1108 8 0.018030984699109354 1108 9 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94-95 -0.2974721048450455 2316 96-97 -0.29544712453956734 2316 98-99 -0.2245796683346093 2316 100-101 -0.25625821079821876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 15.0 5 43.0 6 80.0 7 199.0 8 515.0 9 1035.0 10 1775.0 11 2882.0 12 4421.0 13 6489.0 14 8726.0 15 11743.0 16 15155.0 17 21489.0 18 21622.0 19 15303.0 20 13290.0 21 13325.0 22 13688.0 23 14833.0 24 16942.0 25 19689.0 26 23507.0 27 28882.0 28 35600.0 29 43478.0 30 53723.0 31 66306.0 32 83452.0 33 109449.0 34 153233.0 35 233486.0 36 388119.0 37 660184.0 38 597404.0 39 69570.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.719401702967374 18.859165332870734 15.134392441859406 23.287040522302487 2 15.695600475624257 19.73335069577689 40.01605541044416 24.554993418154687 3 19.37833290537331 23.429138185502847 29.691526900742616 27.501002008381224 4 13.161138187077654 16.744294135552945 36.329000815006594 33.7655668623628 5 14.051426516117745 36.87728459150203 34.10466197592367 14.966626916456551 6 32.88407958624868 35.80839307684446 16.888384101225203 14.41914323568165 7 29.131885232815126 32.48315889428226 19.96235185954974 18.42260401335287 8 26.5934964346514 35.14404139273896 19.016053251655386 19.246408920954256 9 26.14517711631466 17.053041428364647 19.993402825510916 36.80837862980977 10-11 25.249147988977587 26.412032566805266 26.734471142183917 21.604348302033223 12-13 26.005493020801396 23.60195092431058 27.43346385803974 22.959092196848278 14-15 22.754722292230596 25.177503054001733 25.772792454079053 26.294982199688615 16-17 22.37097566958599 28.44195048452257 26.502769155011762 22.68430469087967 18-19 22.267864046280447 26.814062482384255 28.853561026028206 22.064512445307095 20-21 22.755176892071816 25.961378652209667 29.278811901496578 22.004632554221942 22-23 22.5707730124804 25.758263443153744 29.213822308196146 22.457141236169708 24-25 22.377501568452733 26.065702257662686 29.25478037115502 22.302015802729564 26-27 22.187087133403562 26.43006439991386 29.012717746206718 22.370130720475856 28-29 22.06841585870593 26.556409779429856 29.096642341925094 22.278532019939124 30-31 22.316015261462187 26.224791984204654 29.008525019902383 22.450667734430773 32-33 22.128687323546174 26.245346237730743 29.06724365984557 22.55872277887751 34-35 22.63314900888933 26.38890808682576 28.78378604493481 22.194156859350095 36-37 22.685082643861637 26.099490501965946 28.790475889130263 22.424950965042157 38-39 22.24777741928561 26.417128230385256 28.773476639668203 22.56161771066093 40-41 22.676873821495917 26.18235968526666 28.811296761878157 22.329469731359268 42-43 22.465798165851687 26.215983884779888 28.6676374060926 22.65058054327583 44-45 22.43217195228397 26.41904276985743 28.670388420133836 22.47839685772476 46-47 22.64563651433208 26.748763761191796 28.04468243655332 22.56091728792281 48-49 22.646636633982755 26.6051829473417 28.080595824009492 22.667584594666053 50-51 22.62528862543919 26.824063678891036 27.96429100063244 22.58635669503733 52-53 22.77597938080592 26.79860608778287 27.877244223036136 22.548170308375074 54-55 22.626870632886625 26.748054585439498 27.8833540449021 22.741720736771782 56-57 22.601976745581563 26.96548059749694 27.874389336033296 22.55815332088821 58-59 22.672257881033765 26.734725718095003 27.758157248630837 22.834859152240394 60-61 22.492581839791015 26.85983159439802 27.96163613755973 22.685950428251232 62-63 22.427628614477875 26.84948490201191 28.118964050608238 22.603922432901975 64-65 22.27779250681263 26.870978382504674 27.962308945324732 22.888920165357966 66-67 22.22140394250803 26.952115362165145 27.788397230068618 23.038083465258207 68-69 22.407345918390725 26.69321658690538 28.007995888950752 22.89144160575314 70-71 22.42411910370368 26.72614287309282 27.769267668750185 23.080470354453315 72-73 22.491909032026015 26.81455345021277 27.59764713669381 23.095890381067406 74-75 22.357547502955807 27.134464450474255 27.451265987821817 23.056722058748118 76-77 22.476379901450027 26.92329373223197 27.559660773961685 23.040665592356323 78-79 22.506547146913213 26.997302586382155 27.60128393542355 22.89486633128109 80-81 22.571464004239054 27.145520318612665 27.36692862527917 22.916087051869113 82-83 22.530311830260295 27.125410367575153 27.41433605281218 22.929941749352377 84-85 22.604210555793873 27.103591281534932 27.26592460438185 23.026273558289336 86-87 22.575465639049455 27.049506448428932 27.284353495084122 23.09067441743749 88-89 22.529598557208423 27.20856088340799 27.1247508109611 23.137089748422493 90-91 22.59732050946579 27.104361277530405 27.211447069269724 23.08687114373408 92-93 22.52151535605805 26.884575715432867 27.34008591595191 23.25382301255717 94-95 22.420245913056508 26.861304497883566 27.323759824357168 23.394689764702758 96-97 22.540551215036274 26.69964879434667 27.333633732908407 23.42616625770865 98-99 22.39778868090037 26.723215250115377 27.136464689775615 23.74253137920864 100-101 22.46254188228337 26.635913896607992 27.266734820274863 23.634809400833774 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 792.0 1 533.0 2 371.0 3 506.0 4 432.0 5 399.5 6 840.0 7 1990.5 8 3357.5 9 3886.0 10 3263.0 11 2535.0 12 2455.0 13 2662.0 14 2922.0 15 3077.0 16 3205.0 17 3428.5 18 3571.0 19 3891.5 20 4470.0 21 5097.0 22 6114.0 23 7497.5 24 9304.5 25 11885.5 26 15188.5 27 18920.5 28 22304.0 29 25749.5 30 30213.0 31 35494.0 32 42005.5 33 46564.0 34 49504.0 35 53868.5 36 59791.5 37 65631.5 38 69343.0 39 72520.0 40 75808.0 41 80334.0 42 84595.0 43 87759.0 44 92005.5 45 96366.0 46 98355.0 47 101297.5 48 103932.0 49 103900.0 50 103005.5 51 100411.5 52 97837.5 53 97800.0 54 100397.0 55 95548.0 56 85501.5 57 78137.0 58 72733.5 59 64427.0 60 52236.5 61 43059.0 62 35697.5 63 27384.0 64 20677.5 65 15932.5 66 11394.5 67 8693.5 68 6266.0 69 4006.5 70 2871.5 71 1867.5 72 1260.5 73 1002.5 74 733.5 75 512.5 76 342.0 77 180.0 78 93.0 79 55.0 80 25.0 81 15.5 82 10.5 83 5.0 84 2.0 85 2.0 86 2.0 87 0.5 88 0.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016074650385446112 3 0.0070190215483961526 4 0.0 5 0.0 6 0.0 7 0.0 8 3.6367987297389396E-4 9 3.2731188567650457E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0002393013564168E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005309726145418851 26-27 0.02178442439113625 28-29 5.455198094608409E-5 30-31 0.0 32-33 5.091518221634515E-4 34-35 0.019384137229508547 36-37 0.0037095347043337185 38-39 0.01825672962328948 40-41 0.015383658626795714 42-43 0.008019141199074362 44-45 0.0025821270981146475 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.909438983791152E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.27359745947788E-5 84-85 0.002963990964737236 86-87 3.2731188567650457E-4 88-89 5.455198094608409E-5 90-91 2.363919174330311E-4 92-93 2.5457591108172574E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2749671.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91922305829251 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8721537961501 40.370483613893995 2 14.150195906715869 15.259351388255011 3 4.724906633906226 7.6428988416958745 4 2.1234986453449687 4.579895884893493 5 1.1175314029593102 3.012821249540481 6 0.6610905346966486 2.1387292801220674 7 0.4316403782474797 1.6291599684984122 8 0.3088758407932797 1.332347628564039 9 0.23447514410657164 1.1378445837036764 >10 1.2341067273478343 12.126293998877717 >50 0.09208292708390362 3.4089443058656417 >100 0.044518627509409915 4.208053669769182 >500 0.003574548799153398 1.2965010841605709 >1k 0.0011465533883647286 0.9111000269725458 >5k 1.348886339252622E-4 0.5424634430569756 >10k+ 6.74443169626311E-5 0.4031110321302255 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11085 0.4031391391915615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9125 0.3318578840886782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5792 0.2106433824264794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.63679872973894E-5 0.0 6 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 7 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 8 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 9 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 10-11 0.0 0.0 0.0 3.63679872973894E-5 3.63679872973894E-5 12-13 0.0 0.0 0.0 9.091996824347349E-5 3.63679872973894E-5 14-15 0.0 0.0 0.0 1.272879555408629E-4 7.27359745947788E-5 16-17 0.0 0.0 0.0 1.454719491895576E-4 1.0910396189216819E-4 18-19 0.0 0.0 0.0 1.8183993648694698E-4 1.0910396189216819E-4 20-21 0.0 0.0 0.0 2.545759110817258E-4 1.0910396189216819E-4 22-23 0.0 0.0 0.0 4.1823185391997805E-4 1.454719491895576E-4 24-25 0.0 0.0 0.0 6.182557840556198E-4 1.8183993648694698E-4 26-27 0.0 0.0 0.0 0.00120014358081385 1.8183993648694698E-4 28-29 0.0 0.0 0.0 0.0035276947678467715 1.8183993648694698E-4 30-31 0.0 0.0 0.0 0.011437732005028966 1.8183993648694698E-4 32-33 0.0 0.0 0.0 0.02869434197764023 2.1820792378433637E-4 34-35 0.0 0.0 0.0 0.055152052736491014 2.1820792378433637E-4 36-37 0.0 0.0 0.0 0.09781170183632878 2.1820792378433637E-4 38-39 0.0 0.0 0.0 0.16336499893987316 2.1820792378433637E-4 40-41 0.0 0.0 0.0 0.2308639833638279 2.1820792378433637E-4 42-43 1.81839936486947E-5 0.0 0.0 0.29719919219426616 2.363919174330311E-4 44-45 3.63679872973894E-5 0.0 0.0 0.37844527581663406 2.545759110817258E-4 46-47 3.63679872973894E-5 0.0 0.0 0.4726019949295752 3.2731188567650457E-4 48-49 3.63679872973894E-5 0.0 0.0 0.5717411283022586 4.0004786027128335E-4 50-51 3.63679872973894E-5 0.0 0.0 0.6754626280744133 4.0004786027128335E-4 52-53 3.63679872973894E-5 0.0 0.0 0.7714559305458726 4.1823185391997805E-4 54-55 3.63679872973894E-5 0.0 0.0 0.8784505491747923 4.3641584756867275E-4 56-57 3.63679872973894E-5 0.0 0.0 1.0029563536874049 4.3641584756867275E-4 58-59 3.63679872973894E-5 0.0 0.0 1.1347357556594952 4.3641584756867275E-4 60-61 3.63679872973894E-5 0.0 0.0 1.2755162344876896 4.3641584756867275E-4 62-63 3.63679872973894E-5 0.0 0.0 1.4118598188656026 4.7278383486606214E-4 64-65 3.63679872973894E-5 0.0 0.0 1.5532585534778525 4.7278383486606214E-4 66-67 3.63679872973894E-5 0.0 0.0 1.7171690722271866 4.7278383486606214E-4 68-69 3.63679872973894E-5 0.0 0.0 1.8992999526125125 4.7278383486606214E-4 70-71 3.63679872973894E-5 0.0 0.0 2.0878497827558276 4.7278383486606214E-4 72-73 3.63679872973894E-5 0.0 0.0 2.2765632688419815 5.091518221634516E-4 74-75 3.63679872973894E-5 0.0 0.0 2.46493125904881 5.091518221634516E-4 76-77 3.63679872973894E-5 0.0 0.0 2.6717923707963607 5.091518221634516E-4 78-79 3.63679872973894E-5 0.0 0.0 2.9025290662046475 5.091518221634516E-4 80-81 3.63679872973894E-5 0.0 0.0 3.140521175078764 5.637038031095357E-4 82-83 3.63679872973894E-5 0.0 0.0 3.3872961528851997 6.182557840556198E-4 84-85 3.63679872973894E-5 0.0 0.0 3.6362895779167763 6.182557840556198E-4 86-87 3.63679872973894E-5 0.0 0.0 3.8971389668073018 6.546237713530091E-4 88-89 3.63679872973894E-5 0.0 0.0 4.17606324538463 6.546237713530091E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5785 0.0 49.020657 1 GTATCAA 9510 0.0 38.410854 1 ATCAACG 11795 0.0 30.726915 3 TCAACGC 11970 0.0 30.27659 4 TATCAAC 12065 0.0 30.237783 2 CAACGCA 12180 0.0 29.793575 5 AACGCAG 12750 0.0 28.57339 6 ACGCAGA 14565 0.0 24.882305 7 CGCAGAG 14655 0.0 24.535032 8 GTACATG 9080 0.0 22.07673 1 GCAGAGT 16290 0.0 21.897146 9 GAGTACT 9675 0.0 21.62498 12-13 TACATGG 9045 0.0 21.16472 2 ACATGGG 8955 0.0 20.792793 3 TGGTATC 2975 0.0 20.757376 2 GTACTTT 10100 0.0 20.597452 14-15 TAAGGTG 1370 0.0 20.4554 5 GTAAGGT 1100 0.0 20.294659 4 AGAGTAC 14105 0.0 20.288258 10-11 CAGAGTA 15370 0.0 19.947254 10-11 >>END_MODULE