FastQCFastQC Report
Thu 26 May 2016
SRR938176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732955
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.3929080427595771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85730.3136897607168797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.1984664950575476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56050.050.1168141
GTATCAA98650.041.216191
ATCAACG121050.033.277863
TATCAAC121500.033.1937522
TAGCGCG1456.566552E-1032.7996184
TCAACGC123700.032.526514
CAACGCA125300.032.187085
AACGCAG129800.031.0660636
ACGCAGA146850.027.3549777
CGCAGAG147800.027.048018
GCAGAGT158400.024.6373379
GTACATG92550.024.127011
TACATGG91150.023.5840572
GAGTACT94450.022.82427812-13
TAAGGTG13850.022.6637085
TGGTATC29800.022.5029582
GTAAGGT12450.022.1562064
ACATGGG91650.022.0542983
GTACTTT99250.021.55141814-15
CGCGCGT2005.3488657E-721.3927257
Produced by FastQC (version 0.11.5)