##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938176_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2732955 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9603725637634 33.0 31.0 34.0 30.0 34.0 2 32.08383818979822 34.0 31.0 34.0 30.0 34.0 3 32.10576829841691 34.0 31.0 34.0 30.0 34.0 4 35.61420586873915 37.0 35.0 37.0 33.0 37.0 5 35.6159859931832 37.0 35.0 37.0 33.0 37.0 6 35.64301790552717 37.0 35.0 37.0 33.0 37.0 7 35.57579652793405 37.0 35.0 37.0 33.0 37.0 8 35.56918573485476 37.0 35.0 37.0 33.0 37.0 9 37.20017124321476 39.0 37.0 39.0 34.0 39.0 10-11 37.108588871752374 39.0 37.0 39.0 34.0 39.0 12-13 36.95870349127593 39.0 37.0 39.0 33.0 39.0 14-15 38.14605363059399 40.0 38.0 41.0 33.0 41.0 16-17 37.96476304951966 40.0 38.0 41.0 33.0 41.0 18-19 38.13605785678871 40.0 38.0 41.0 33.0 41.0 20-21 38.13616433494148 40.0 38.0 41.0 33.0 41.0 22-23 38.02232601707675 40.0 38.0 41.0 33.0 41.0 24-25 37.903244656425 40.0 38.0 41.0 33.0 41.0 26-27 37.723518499206904 40.0 38.0 41.0 32.0 41.0 28-29 37.544140316982904 40.0 37.5 41.0 32.0 41.0 30-31 37.32424628286964 40.0 37.0 41.0 31.5 41.0 32-33 37.04082376036195 40.0 36.5 41.0 31.0 41.0 34-35 36.80073967555265 39.5 36.0 41.0 30.0 41.0 36-37 36.53624099189339 39.0 36.0 41.0 30.0 41.0 38-39 36.24663304006103 39.0 35.0 40.5 30.0 41.0 40-41 36.13318459323333 39.0 35.0 41.0 29.5 41.0 42-43 35.915065194999556 39.0 35.0 41.0 28.5 41.0 44-45 35.588936334480444 39.0 35.0 40.0 27.0 41.0 46-47 35.20922646000392 38.5 35.0 40.0 25.5 41.0 48-49 34.80551399492491 38.0 34.0 40.0 24.0 41.0 50-51 34.07499702702752 37.5 33.5 39.5 23.5 40.5 52-53 34.14090938196934 38.0 33.5 40.0 23.0 40.5 54-55 34.21372525343447 38.0 34.0 40.0 22.5 41.0 56-57 33.89222361875699 38.0 33.5 40.0 21.5 41.0 58-59 33.480865583224016 37.5 33.0 40.0 19.0 41.0 60-61 32.96931599678736 37.0 32.5 40.0 16.0 41.0 62-63 32.42100528548769 36.0 31.5 39.0 11.0 41.0 64-65 31.876803130677235 36.0 31.0 39.0 7.5 40.5 66-67 31.283332327096495 35.0 30.5 39.0 4.0 40.0 68-69 30.595562678492694 35.0 29.5 38.0 2.0 40.0 70-71 30.00442451485663 34.5 29.0 37.5 2.0 39.5 72-73 29.292765523032763 34.0 28.0 37.0 2.0 39.0 74-75 28.60509247316549 34.0 26.5 36.0 2.0 39.0 76-77 27.854136639644636 33.5 26.0 35.5 2.0 38.0 78-79 27.0839453997596 33.0 25.0 35.0 2.0 37.0 80-81 26.347250137671494 32.0 23.0 35.0 2.0 36.5 82-83 25.599571709010945 32.0 20.0 35.0 2.0 36.0 84-85 24.86118706674643 31.0 17.5 34.5 2.0 36.0 86-87 24.10522657709329 31.0 9.5 34.0 2.0 35.0 88-89 23.219518616296277 30.5 3.5 34.0 2.0 35.0 90-91 22.311114892122262 29.5 2.0 34.0 2.0 35.0 92-93 21.35538693465498 29.0 2.0 34.0 2.0 35.0 94-95 20.270250699334603 28.0 2.0 34.0 2.0 35.0 96-97 18.993829389799686 25.5 2.0 33.0 2.0 35.0 98-99 17.426070498782455 21.5 2.0 33.0 2.0 34.5 100-101 13.493191435643837 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10441474486307811 1101 2 0.1175628486742113 1101 3 0.18010932413406522 1101 4 0.05322377550427149 1101 5 0.039038185966298045 1101 6 0.09518091336067158 1101 7 0.1343026866351451 1101 8 0.21321386525738006 1101 9 0.20188406194645125 1101 10-11 0.16292785157903467 1101 12-13 0.19099550287211997 1101 14-15 0.23690753260406439 1101 16-17 0.356507276771481 1101 18-19 0.2527859971155806 1101 20-21 0.2644837361899093 1101 22-23 0.20426538600938215 1101 24-25 0.14065738453180643 1101 26-27 0.11767009365402004 1101 28-29 0.16963012208555028 1101 30-31 0.06884274399831014 1101 32-33 0.0753422917681732 1101 34-35 0.07245389553578008 1101 36-37 0.11627360579254287 1101 38-39 0.15060150339252942 1101 40-41 0.08693353292631656 1101 42-43 0.2766404796886377 1101 44-45 0.20814598962596875 1101 46-47 0.061485906582113614 1101 48-49 0.36930625798409267 1101 50-51 0.2400609926258852 1101 52-53 0.21467718906044553 1101 54-55 0.24188756929309818 1101 56-57 0.3406135295617929 1101 58-59 0.19025239429598173 1101 60-61 0.13923684468382191 1101 62-63 0.14860841478890308 1101 64-65 0.1013641612293803 1101 66-67 0.00689906117068162 1101 68-69 -0.03712528442976648 1101 70-71 -0.046242459900675215 1101 72-73 -0.23453475872219798 1101 74-75 -0.20486734813826857 1101 76-77 -0.2847597722576154 1101 78-79 -0.25445692509623896 1101 80-81 -0.47820287763489233 1101 82-83 -0.4801486819242875 1101 84-85 -0.5130651536724713 1101 86-87 -0.5414344718384037 1101 88-89 -0.8191386381201156 1101 90-91 -0.9636507889332648 1101 92-93 -1.0157854501400365 1101 94-95 -1.414371981586715 1101 96-97 -1.4594530910866688 1101 98-99 -1.513989232807461 1101 100-101 -1.617733800043414 1102 1 0.11752424442164511 1102 2 0.13019440581887665 1102 3 0.15986831619166253 1102 4 0.1530535009678644 1102 5 0.12693971843712148 1102 6 0.17624712711261026 1102 7 0.1460356637123823 1102 8 0.19298804652856205 1102 9 0.23796017026280225 1102 10-11 0.18124725139796993 1102 12-13 0.1506651202161251 1102 14-15 0.1393176983304656 1102 16-17 0.16676163568742197 1102 18-19 0.3155991775556686 1102 20-21 0.18442734623446455 1102 22-23 0.18976503777940223 1102 24-25 0.18092631991465424 1102 26-27 0.19384848751550976 1102 28-29 0.10668947307460996 1102 30-31 0.0015772253255050828 1102 32-33 -0.035653318031286574 1102 34-35 0.11666115235517793 1102 36-37 -0.02163444250570734 1102 38-39 -0.12997528973296824 1102 40-41 0.0737181403843934 1102 42-43 0.18457080262339787 1102 44-45 0.2188710415584083 1102 46-47 0.14108610231584606 1102 48-49 0.02053818087811976 1102 50-51 -0.12304423496635764 1102 52-53 -0.21743712358016865 1102 54-55 -0.013912336982542683 1102 56-57 -0.04135834105026959 1102 58-59 -0.08171927782068167 1102 60-61 -0.11668353967232292 1102 62-63 -0.10337537417768061 1102 64-65 -0.09133716273496972 1102 66-67 -0.1098100231009731 1102 68-69 -0.3294596508022245 1102 70-71 -0.5763005327519437 1102 72-73 -0.720139008940329 1102 74-75 -0.8075160535598656 1102 76-77 -0.7964924904277986 1102 78-79 -1.0575430108272705 1102 80-81 -1.0273470603818424 1102 82-83 -1.1198511286822104 1102 84-85 -1.049175437719395 1102 86-87 -0.9659635846416172 1102 88-89 -0.9327370404910127 1102 90-91 -1.139679707124678 1102 92-93 -1.3257232520364433 1102 94-95 -1.372751551404022 1102 96-97 -1.5956937779843337 1102 98-99 -1.7413834973258915 1102 100-101 -1.3493818210873787 1103 1 0.25611370545149725 1103 2 0.3043804406816335 1103 3 0.3346596071821111 1103 4 0.22409554331074588 1103 5 0.2308692916689381 1103 6 0.17708029347873122 1103 7 0.31694246261707804 1103 8 0.32771806055380637 1103 9 0.2660732738492584 1103 10-11 0.3058348423950932 1103 12-13 0.2631041771853333 1103 14-15 0.35674030902971765 1103 16-17 0.35568586596859575 1103 18-19 0.3155052585013678 1103 20-21 0.28077076438713533 1103 22-23 0.2884013331109969 1103 24-25 0.27036272210525425 1103 26-27 0.27854803002536244 1103 28-29 0.3352517184496193 1103 30-31 0.40163900319232937 1103 32-33 0.4027892118558398 1103 34-35 0.22038994751980567 1103 36-37 0.09150337222747851 1103 38-39 0.029267597463984885 1103 40-41 0.3341707050007017 1103 42-43 0.32261553313831826 1103 44-45 0.22675690277526428 1103 46-47 0.13259206304515203 1103 48-49 0.15676171657570848 1103 50-51 0.171164090018614 1103 52-53 0.1720602216412246 1103 54-55 0.15829889323184432 1103 56-57 0.1661697929242223 1103 58-59 0.1941652309617936 1103 60-61 0.016294153508731313 1103 62-63 -0.044065700097668525 1103 64-65 -0.10283869883327768 1103 66-67 -0.13549124930814216 1103 68-69 -0.06920661199904643 1103 70-71 -0.33998766959826554 1103 72-73 -0.3485714373113993 1103 74-75 -0.23711600013036005 1103 76-77 -0.1742153081663922 1103 78-79 -0.2224807320588198 1103 80-81 -0.3098142513255411 1103 82-83 -0.4374544813837389 1103 84-85 -0.7939047003922042 1103 86-87 -0.9439990484765204 1103 88-89 -0.7408007217840051 1103 90-91 -0.671523692965863 1103 92-93 -0.842586940008129 1103 94-95 -0.9341028170154573 1103 96-97 -1.178523705186631 1103 98-99 -1.1941739367835815 1103 100-101 -1.3379537505604304 1104 1 0.1391626530165766 1104 2 0.152034258093515 1104 3 0.0757543885994707 1104 4 0.08440037579299542 1104 5 0.07110624521234854 1104 6 0.03574575617645337 1104 7 0.012584821565887694 1104 8 0.1767886172856592 1104 9 0.148183437007809 1104 10-11 0.19253928877696325 1104 12-13 0.03844979930514825 1104 14-15 0.07117937367040383 1104 16-17 0.191074768696204 1104 18-19 0.2405278329191063 1104 20-21 0.1235158135838006 1104 22-23 0.26277158469898865 1104 24-25 0.28315618953582344 1104 26-27 0.08775761403332893 1104 28-29 0.1011861436582393 1104 30-31 0.020224754842601556 1104 32-33 -0.017126095156235976 1104 34-35 0.05200892248555533 1104 36-37 0.06824860824691115 1104 38-39 -0.12610589238531134 1104 40-41 -0.14422713408261956 1104 42-43 -0.3287412672104182 1104 44-45 -0.12731271984274173 1104 46-47 -0.3411616734101983 1104 48-49 -0.3598686234417272 1104 50-51 -0.37863429707815754 1104 52-53 -0.32345525351134796 1104 54-55 -0.344431054457786 1104 56-57 -0.42974735117840623 1104 58-59 -0.24469001253054756 1104 60-61 -0.33196528104101475 1104 62-63 -0.13106828447929075 1104 64-65 -0.33355080494861156 1104 66-67 -0.431658268862261 1104 68-69 -0.35207729950683486 1104 70-71 -0.37232202007777815 1104 72-73 -0.5674195192643197 1104 74-75 -0.7348928091974933 1104 76-77 -0.8551601643128635 1104 78-79 -0.763723414833759 1104 80-81 -0.9383257409257411 1104 82-83 -1.0028767008382182 1104 84-85 -0.951878140422096 1104 86-87 -0.9285706015964621 1104 88-89 -0.8699341755142349 1104 90-91 -0.9794200686041705 1104 92-93 -1.2607772501339234 1104 94-95 -1.2724186097667314 1104 96-97 -1.6625674839884717 1104 98-99 -1.787083234329975 1104 100-101 -2.023395586405492 1105 1 0.1886906709856575 1105 2 0.191914170597002 1105 3 0.3202293361162418 1105 4 0.13857334289372147 1105 5 0.18742669385218846 1105 6 0.15354601161929793 1105 7 0.20296754540868278 1105 8 0.21418163224521436 1105 9 0.23904181303728933 1105 10-11 0.17729933399328246 1105 12-13 0.23022057605691515 1105 14-15 0.2989096025413147 1105 16-17 0.315705184011037 1105 18-19 0.3753091650388427 1105 20-21 0.24672808071242258 1105 22-23 0.25958295337347437 1105 24-25 0.241397157282492 1105 26-27 0.20054223461263376 1105 28-29 0.29747268005884564 1105 30-31 0.13394306304759596 1105 32-33 0.24306886351780577 1105 34-35 0.2531378623977645 1105 36-37 0.3054353604526696 1105 38-39 0.35748190508081024 1105 40-41 0.2360802781639535 1105 42-43 0.372815612863576 1105 44-45 0.21904501757963146 1105 46-47 0.4033029976704441 1105 48-49 0.4008651140980959 1105 50-51 0.2453200755454148 1105 52-53 0.32982806783280694 1105 54-55 0.42184682037522236 1105 56-57 0.37725236988975297 1105 58-59 0.2733209098529983 1105 60-61 0.3292433746543253 1105 62-63 0.41440127524882797 1105 64-65 0.281358835816544 1105 66-67 0.23731937254884272 1105 68-69 0.2399870897058456 1105 70-71 -0.051383781458930855 1105 72-73 -0.212959545983054 1105 74-75 -0.152398718148266 1105 76-77 -0.1729435370392025 1105 78-79 -0.3393932182602164 1105 80-81 -0.28444858837938725 1105 82-83 -0.24414833640642897 1105 84-85 -0.22068548660586984 1105 86-87 -0.3304181580067542 1105 88-89 -0.27556859863915406 1105 90-91 -0.32500707331665524 1105 92-93 -0.6489710930806147 1105 94-95 -0.6701922932818576 1105 96-97 -0.41558214993089493 1105 98-99 -0.5302273833676594 1105 100-101 -0.9477676227547143 1106 1 0.31834942907835284 1106 2 0.22135134303560022 1106 3 0.27025245809754495 1106 4 0.11493850320612609 1106 5 0.2779595270656259 1106 6 0.24665309208378972 1106 7 0.2705360763921405 1106 8 0.15320029682233383 1106 9 0.36925003932441314 1106 10-11 0.30786868066885376 1106 12-13 0.41781755992291636 1106 14-15 0.49448415166267523 1106 16-17 0.019984035105473197 1106 18-19 0.3375355549268235 1106 20-21 0.4461750799930684 1106 22-23 0.4871140074789153 1106 24-25 0.3701433999605328 1106 26-27 0.27230756577078097 1106 28-29 0.283335033619629 1106 30-31 0.04939946526731376 1106 32-33 0.3006164241487781 1106 34-35 0.24287523522686882 1106 36-37 0.2699498630731654 1106 38-39 0.28626138560697 1106 40-41 0.19506461302162137 1106 42-43 -0.049180456399597006 1106 44-45 0.2606578400028141 1106 46-47 0.369637071763556 1106 48-49 0.5157648225042237 1106 50-51 0.16042820292184246 1106 52-53 0.1788673816237889 1106 54-55 0.11857570229896908 1106 56-57 -0.0480629882440482 1106 58-59 0.06267002607949479 1106 60-61 0.3174193714686311 1106 62-63 0.19905357845507154 1106 64-65 0.04908071049540652 1106 66-67 -0.08607429202828243 1106 68-69 -0.0817406682327011 1106 70-71 -0.20158722278048202 1106 72-73 -0.2550884381832361 1106 74-75 -0.2178319983866821 1106 76-77 -0.45283752917772446 1106 78-79 -0.43331197660981857 1106 80-81 -0.5576428258678234 1106 82-83 -0.5903465367455532 1106 84-85 -0.4847871655186218 1106 86-87 -0.815799124762485 1106 88-89 -0.7880326311126922 1106 90-91 -0.6362673176389215 1106 92-93 -0.5993872887439586 1106 94-95 -0.7794589089945312 1106 96-97 -0.8573189318649206 1106 98-99 -1.1173498849091246 1106 100-101 -1.4324025844749908 1107 1 0.016539854933654397 1107 2 -0.049736750425957155 1107 3 -0.12808529683530523 1107 4 0.015444741806689422 1107 5 -0.04016304335755905 1107 6 -0.07515875043357312 1107 7 0.03340160679984905 1107 8 0.1087433590563478 1107 9 0.049558607094638774 1107 10-11 0.037461818668816704 1107 12-13 -0.05965128161801658 1107 14-15 -0.061096592041145925 1107 16-17 -0.03554733982774394 1107 18-19 0.01213116280464277 1107 20-21 0.08824487217788857 1107 22-23 0.12796570081335545 1107 24-25 0.058750670958524154 1107 26-27 0.04878779408191747 1107 28-29 0.053643158078443776 1107 30-31 -0.07303335866090777 1107 32-33 -0.08396673070853211 1107 34-35 -0.03400526874336407 1107 36-37 -0.08683320450361265 1107 38-39 -0.04645735690855446 1107 40-41 -0.19088935283073027 1107 42-43 -0.20927517535168505 1107 44-45 -0.18439543796840496 1107 46-47 -0.19671657098962925 1107 48-49 -0.18462129021892792 1107 50-51 -0.27996354043194316 1107 52-53 -0.28062561405594266 1107 54-55 -0.3881689641466153 1107 56-57 -0.4045872655888729 1107 58-59 -0.41692210439454414 1107 60-61 -0.4497848534618214 1107 62-63 -0.4063475953414226 1107 64-65 -0.50845664856611 1107 66-67 -0.5962675758028588 1107 68-69 -0.6010165104092344 1107 70-71 -0.6069910449308509 1107 72-73 -0.7400590299406389 1107 74-75 -0.8449639232563335 1107 76-77 -0.9310716586292678 1107 78-79 -1.0739823152767087 1107 80-81 -1.0688324088557195 1107 82-83 -1.0757744013432777 1107 84-85 -1.2884441104236508 1107 86-87 -1.059173202444459 1107 88-89 -1.019504517109116 1107 90-91 -1.127404687754435 1107 92-93 -1.3108687088143363 1107 94-95 -1.4229111706887387 1107 96-97 -1.4580977206230052 1107 98-99 -1.4874698610969688 1107 100-101 -1.2822390486231878 1108 1 -0.08162734745878808 1108 2 0.03422742448641003 1108 3 -0.06551028823292171 1108 4 0.0928871897517709 1108 5 0.04804031236528772 1108 6 0.04417684702888636 1108 7 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54-55 -0.31744027214850234 2316 56-57 -0.3184946607730268 2316 58-59 -0.3142016737977684 2316 60-61 -0.36059354791981946 2316 62-63 -0.32060885914634696 2316 64-65 -0.2607701172639274 2316 66-67 -0.17631205531732874 2316 68-69 -0.239978271406585 2316 70-71 -0.22257202625585393 2316 72-73 -0.1746944107361017 2316 74-75 -0.12449592953818822 2316 76-77 -0.17709522036861358 2316 78-79 -0.08142384047383544 2316 80-81 -0.053191135340050266 2316 82-83 -0.09106004027117365 2316 84-85 -0.24813046014287465 2316 86-87 -0.19212683209874726 2316 88-89 -0.26526796229880034 2316 90-91 -0.24999406490203313 2316 92-93 -0.2271313845618117 2316 94-95 -0.11058188515951173 2316 96-97 -0.2711101756765508 2316 98-99 -0.4325469372456716 2316 100-101 -0.39772149937815904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9431.0 3 2753.0 4 2665.0 5 3017.0 6 3476.0 7 4305.0 8 5441.0 9 6899.0 10 8443.0 11 10682.0 12 13240.0 13 16275.0 14 19010.0 15 22915.0 16 26549.0 17 32886.0 18 33176.0 19 28842.0 20 30070.0 21 31779.0 22 34937.0 23 38562.0 24 43788.0 25 48711.0 26 54861.0 27 61901.0 28 72011.0 29 82711.0 30 96714.0 31 114912.0 32 139890.0 33 172363.0 34 219315.0 35 285956.0 36 361668.0 37 385275.0 38 195817.0 39 11708.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.475010937455345 18.413471720540507 14.781833491257732 23.32968385074642 2 15.461395658395846 19.380422474832248 40.650343703312124 24.507838163459784 3 19.364871471070455 23.479167781319422 29.38326049306619 27.772700254543935 4 12.929382635718218 16.383348684456383 36.63323858869322 34.054030091132184 5 14.110275156304647 36.73289374216827 34.04974032934876 15.107090772178323 6 32.74747199960697 35.62250531704082 17.06948834320605 14.56053434014616 7 29.082881951100088 32.4882690708459 20.07336709827592 18.355481879778086 8 26.50976681786426 34.93956978254788 19.198091307334376 19.352572092253478 9 26.302211743259885 17.063071353576326 20.095856617315736 36.53886028584805 10-11 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26.357012173404122 28.780504241198102 22.238945334224283 36-37 22.76517162957456 26.037596270403103 28.675597581475067 22.521634518547273 38-39 22.30402677426257 26.3694233938362 28.68908947701851 22.637460354882716 40-41 22.772301333201533 26.152369027548193 28.690467691417755 22.38486194783252 42-43 22.51139745294569 26.234608129719344 28.53696318838712 22.717031228947846 44-45 22.461481021218496 26.445894984594236 28.58681537702707 22.5058086171602 46-47 22.716811750559796 26.72925070302706 27.974884869766786 22.57905267664636 48-49 22.72018203292428 26.61894745010961 27.94936403967548 22.711506477290637 50-51 22.637858873296413 26.850060542493694 27.837374533346882 22.674706050863016 52-53 22.830913772374 26.780230177015607 27.786490596560704 22.602365454049693 54-55 22.67471977220818 26.748387493561506 27.807943060831736 22.768949673398577 56-57 22.625114465764216 26.998054356506596 27.755724386203646 22.621106791525545 58-59 22.771951018615123 26.688960884447127 27.6732377866857 22.865850310252046 60-61 22.511308262833037 26.821799347538626 27.9140361553279 22.752856234300435 62-63 22.48695122140961 26.75124538842482 28.072038959172858 22.689764430992714 64-65 22.409037358370323 26.789314926638173 27.9574560530941 22.844191661897405 66-67 22.387045446101602 26.79892992204654 27.793018211713573 23.021006420138285 68-69 22.585896878710578 26.56263100873569 27.957561682345304 22.89391043020843 70-71 22.577003165937906 26.561827311412898 27.775998178341 23.085171344308193 72-73 22.620403450448404 26.65150332023106 27.61823072082273 23.10986250849781 74-75 22.532753521434817 26.97419344093128 27.46231384335378 23.03073919428012 76-77 22.68336341690858 26.770118299450886 27.43898377536426 23.107534508276277 78-79 22.71757336072346 26.872187768024958 27.4852449602845 22.924993910967085 80-81 22.77445707719749 26.95753331980369 27.298312045490352 22.96969755750847 82-83 22.805791177819344 26.950610149529915 27.275566972616744 22.968031700034 84-85 22.869815495967238 26.90592470045474 27.169462678597668 23.054797124980354 86-87 22.903933343385795 26.753428939089392 27.191314964652907 23.151322752871906 88-89 22.87644720495985 26.88545345968989 27.04228853626575 23.19581079908451 90-91 22.975586321864068 26.834093618458414 27.034129772218492 23.156190287459022 92-93 23.008062082724233 26.487801412622197 27.13803020937393 23.36610629527964 94-95 22.878637523755817 26.55283403700598 27.102870471747515 23.465657967490692 96-97 23.02168559737502 26.272538856878498 27.113962662905607 23.591812882840877 98-99 22.897616682326756 26.231211779903497 26.890400043493134 23.980771494276606 100-101 23.84527677927967 25.167080253112704 26.90361206971408 24.08403089789355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 776.0 1 538.5 2 393.0 3 491.0 4 418.5 5 423.0 6 788.0 7 1731.0 8 2959.0 9 3492.5 10 3015.0 11 2451.5 12 2433.5 13 2597.0 14 2745.0 15 2963.5 16 3131.5 17 3388.5 18 3681.5 19 4050.0 20 4652.0 21 5389.5 22 6444.5 23 7887.0 24 9621.0 25 12127.5 26 15616.0 27 19151.0 28 22186.0 29 25670.5 30 29895.5 31 35150.0 32 41441.0 33 45742.0 34 49028.5 35 53418.5 36 59018.0 37 64683.0 38 68448.0 39 71652.0 40 75255.5 41 79596.0 42 83326.5 43 86504.0 44 90394.0 45 94356.0 46 96513.0 47 98827.0 48 101168.5 49 101695.0 50 100283.5 51 97749.5 52 95912.0 53 95425.0 54 97537.0 55 93759.0 56 84888.0 57 77999.5 58 72568.0 59 64611.5 60 53618.5 61 45019.0 62 37393.0 63 29334.0 64 22954.0 65 17969.5 66 13252.0 67 10201.0 68 7628.0 69 5110.5 70 3750.5 71 2667.5 72 1885.5 73 1411.5 74 979.5 75 682.5 76 498.0 77 314.0 78 196.5 79 125.5 80 87.0 81 61.0 82 42.0 83 28.0 84 13.0 85 7.0 86 10.5 87 11.5 88 6.5 89 6.0 90 6.0 91 3.0 92 2.0 93 1.5 94 1.5 95 1.5 96 2.5 97 2.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1390802263484031 2 0.05064115581851879 3 0.15196005788606107 4 0.12854218236304657 5 0.19279497832931752 6 0.1977346864474534 7 0.1640714903831201 8 0.0684606954743126 9 0.11902867043182197 10-11 0.10022118915240098 12-13 0.14939872775073135 14-15 0.13531141200641797 16-17 0.13262201536432178 18-19 0.13185361632372286 20-21 0.13194509239998461 22-23 0.19550267018666606 24-25 0.21451139883386297 26-27 0.14614217943581215 28-29 0.12718833643437233 30-31 0.09815382982888486 32-33 0.12243158046876002 34-35 0.09277503654469246 36-37 0.0865363681436394 38-39 0.05036672758973346 40-41 0.057081071587347765 42-43 0.0601180773192387 44-45 0.03741371519106608 46-47 0.05029354672872404 48-49 0.04176797642112658 50-51 0.05131807878285592 52-53 0.025979205658344173 54-55 0.04895799601530212 56-57 0.025503530061782943 58-59 0.04775051180864669 60-61 0.036316002275924776 62-63 0.05040331802023816 64-65 0.03843824724519796 66-67 0.0367550874419813 68-69 0.06381371080021442 70-71 0.05045820366599524 72-73 0.05005570893044342 74-75 0.04206069986516426 76-77 0.02074677409617063 78-79 0.021350516199498345 80-81 0.025009559249969355 82-83 0.02166153485878838 84-85 0.01908190950820632 86-87 0.022795838204434395 88-89 0.063740529939205 90-91 0.10788688434313774 92-93 0.07855965429361259 94-95 0.07482743038213216 96-97 0.06624697442877764 98-99 0.0545014462367657 100-101 0.13092056034585275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2732955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.469868565766866 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98060505529675 43.470846495858154 2 13.17373789590297 14.878384950030057 3 4.238273915391583 7.180043128436424 4 1.8805570581590818 4.247792396186742 5 1.019520967116845 2.878610750656588 6 0.6043347955844144 2.0476023885822876 7 0.3917366641777798 1.5484922556957825 8 0.2743405970815964 1.2393581967561402 9 0.20432401358292415 1.0384335172671921 >10 1.107818543517935 11.439117571511304 >50 0.07944301089703748 3.0730022242089006 >100 0.04121295832364172 4.073348305842297 >500 0.0029896531508507703 1.2105142490334002 >1k 9.098944371985029E-4 0.766650460774269 >5k 1.2998491959978614E-4 0.5137060893033388 >10k+ 6.499245979989307E-5 0.3940970198570588 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10738 0.3929080427595771 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8573 0.3136897607168797 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5424 0.1984664950575476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.31808610094202E-5 0.0 6 0.0 0.0 0.0 7.31808610094202E-5 0.0 7 0.0 0.0 0.0 7.31808610094202E-5 0.0 8 0.0 0.0 0.0 7.31808610094202E-5 0.0 9 0.0 0.0 0.0 7.31808610094202E-5 0.0 10-11 0.0 0.0 0.0 7.31808610094202E-5 0.0 12-13 0.0 0.0 0.0 1.0977129151413032E-4 0.0 14-15 0.0 0.0 0.0 1.2806650676648536E-4 0.0 16-17 0.0 0.0 0.0 1.463617220188404E-4 0.0 18-19 0.0 0.0 0.0 2.012473677759056E-4 0.0 20-21 0.0 0.0 0.0 2.1954258302826063E-4 0.0 22-23 0.0 0.0 0.0 4.5738038130887625E-4 1.829521525235505E-5 24-25 0.0 0.0 0.0 8.415799016083323E-4 5.488564575706515E-5 26-27 0.0 0.0 0.0 0.0011891889914030784 7.31808610094202E-5 28-29 0.0 0.0 0.0 0.0033663196064333293 7.31808610094202E-5 30-31 0.0 0.0 0.0 0.011160081303936581 9.147607626177526E-5 32-33 0.0 0.0 0.0 0.02806486019711265 1.0977129151413032E-4 34-35 0.0 0.0 0.0 0.053348847675867325 1.463617220188404E-4 36-37 0.0 0.0 0.0 0.09709270734424826 1.463617220188404E-4 38-39 0.0 0.0 0.0 0.16065028513092972 1.463617220188404E-4 40-41 0.0 0.0 0.0 0.22746441123253036 1.463617220188404E-4 42-43 0.0 0.0 0.0 0.29457126077816864 1.463617220188404E-4 44-45 0.0 0.0 0.0 0.37600326386640104 1.6465693727119545E-4 46-47 0.0 0.0 0.0 0.47265688604459277 1.8295215252355052E-4 48-49 0.0 0.0 0.0 0.5722011522326567 1.8295215252355052E-4 50-51 0.0 0.0 0.0 0.6764838791710804 1.8295215252355052E-4 52-53 0.0 0.0 0.0 0.7762293927269202 2.1954258302826063E-4 54-55 0.0 0.0 0.0 0.8856896655817603 2.7442822878532576E-4 56-57 0.0 0.0 0.0 1.008871349875867 2.927234440376808E-4 58-59 0.0 0.0 0.0 1.1382551121405218 2.927234440376808E-4 60-61 0.0 0.0 0.0 1.276402282511055 2.927234440376808E-4 62-63 0.0 0.0 0.0 1.4176047538287313 2.927234440376808E-4 64-65 0.0 0.0 0.0 1.5634359146052534 2.927234440376808E-4 66-67 0.0 0.0 0.0 1.7250924365750626 3.110186592900359E-4 68-69 0.0 0.0 0.0 1.902281596294121 3.2931387454239095E-4 70-71 0.0 0.0 0.0 2.08770360287674 3.6590430504710104E-4 72-73 0.0 0.0 0.0 2.2740220750067235 4.7567559656123135E-4 74-75 0.0 0.0 0.0 2.4622615447382046 4.939708118135864E-4 76-77 0.0 0.0 0.0 2.668229078049218 5.122660270659414E-4 78-79 0.0 0.0 0.0 2.890076858199275 5.122660270659414E-4 80-81 0.0 0.0 0.0 3.128646465089985 5.122660270659414E-4 82-83 0.0 0.0 0.0 3.371186133690456 5.488564575706515E-4 84-85 0.0 0.0 0.0 3.6164334941482754 5.488564575706515E-4 86-87 0.0 0.0 0.0 3.8659619349751457 5.488564575706515E-4 88-89 0.0 0.0 0.0 4.1376458814726185 5.488564575706515E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5605 0.0 50.116814 1 GTATCAA 9865 0.0 41.21619 1 ATCAACG 12105 0.0 33.27786 3 TATCAAC 12150 0.0 33.193752 2 TAGCGCG 145 6.566552E-10 32.799618 4 TCAACGC 12370 0.0 32.52651 4 CAACGCA 12530 0.0 32.18708 5 AACGCAG 12980 0.0 31.066063 6 ACGCAGA 14685 0.0 27.354977 7 CGCAGAG 14780 0.0 27.04801 8 GCAGAGT 15840 0.0 24.637337 9 GTACATG 9255 0.0 24.12701 1 TACATGG 9115 0.0 23.584057 2 GAGTACT 9445 0.0 22.824278 12-13 TAAGGTG 1385 0.0 22.663708 5 TGGTATC 2980 0.0 22.502958 2 GTAAGGT 1245 0.0 22.156206 4 ACATGGG 9165 0.0 22.054298 3 GTACTTT 9925 0.0 21.551418 14-15 CGCGCGT 200 5.3488657E-7 21.392725 7 >>END_MODULE