FastQCFastQC Report
Thu 26 May 2016
SRR938176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732955
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104320.3817113710251358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88720.32463029943778804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55100.20161327208095264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.048.9939881
GTATCAA94150.041.073211
ATCAACG117400.032.93063
TATCAAC118750.032.6009982
TCAACGC120900.031.9356524
CAACGCA122950.031.3645485
AACGCAG126850.030.4376856
ACGCAGA144350.026.6484437
CGCAGAG144350.026.5497468
GCAGAGT157700.023.8504779
GTACATG88200.023.4301621
GAGTACT94550.023.18001712-13
TACATGG89450.022.5691642
TAAGGTG15700.021.4768335
ACATGGG90300.021.2489243
AGAGTAC139600.021.09161610-11
GTACTTT103600.020.97137814-15
TGGTATC27650.020.4436652
CAGAGTA153200.020.3197110-11
AGTACTT100750.020.03306812-13
Produced by FastQC (version 0.11.5)