##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2732955 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53234648942262 34.0 31.0 34.0 31.0 34.0 2 32.74787107727716 34.0 31.0 34.0 31.0 34.0 3 32.80136921390949 34.0 31.0 34.0 31.0 34.0 4 36.251128906257144 37.0 35.0 37.0 35.0 37.0 5 36.22217745992891 37.0 37.0 37.0 35.0 37.0 6 36.252978186614854 37.0 37.0 37.0 35.0 37.0 7 36.2135428501384 37.0 37.0 37.0 35.0 37.0 8 36.202592066096955 37.0 37.0 37.0 35.0 37.0 9 37.89441465373561 39.0 38.0 39.0 35.0 39.0 10-11 37.875395313863564 39.0 38.0 39.0 35.0 39.0 12-13 37.79325144394986 39.0 38.0 39.0 35.0 39.0 14-15 39.08957867948796 40.0 38.5 41.0 36.0 41.0 16-17 38.97330325599946 40.0 38.0 41.0 36.0 41.0 18-19 39.21695490778296 40.5 39.0 41.0 36.0 41.0 20-21 39.19506102369047 40.5 39.0 41.0 36.0 41.0 22-23 39.103671300844695 40.0 39.0 41.0 35.5 41.0 24-25 39.006171524961076 40.0 38.5 41.0 35.0 41.0 26-27 38.843288125856446 40.0 38.0 41.0 35.0 41.0 28-29 38.730511296380655 40.0 38.0 41.0 35.0 41.0 30-31 38.50281325524936 40.0 38.0 41.0 34.0 41.0 32-33 38.30187946746287 40.0 38.0 41.0 34.0 41.0 34-35 38.147472424536815 40.0 38.0 41.0 34.0 41.0 36-37 38.15195676474732 40.0 38.0 41.0 34.0 41.0 38-39 38.018403705878804 40.0 38.0 41.0 33.0 41.0 40-41 37.85139400392615 40.0 37.5 41.0 33.0 41.0 42-43 37.62434837017075 40.0 37.0 41.0 33.0 41.0 44-45 37.360328106390334 40.0 37.0 41.0 32.5 41.0 46-47 36.98083539611885 40.0 36.5 41.0 31.0 41.0 48-49 36.59913335565349 39.5 36.0 41.0 30.5 41.0 50-51 36.22697702669821 39.0 35.5 41.0 30.0 41.0 52-53 35.71399620557236 39.0 35.0 40.0 28.0 41.0 54-55 35.39795660740847 38.0 35.0 40.0 28.0 41.0 56-57 35.057912040264114 38.0 34.0 40.0 27.0 41.0 58-59 34.62586229923288 38.0 34.0 40.0 26.0 41.0 60-61 34.148931285001034 37.0 33.5 40.0 25.5 41.0 62-63 33.68466001086736 36.5 33.0 39.5 23.5 41.0 64-65 33.19282754381247 36.0 32.0 39.0 22.5 40.0 66-67 32.6389029457126 35.5 31.5 39.0 21.5 40.0 68-69 32.05114866508962 35.0 31.0 38.0 19.0 40.0 70-71 31.44242825073958 35.0 30.5 37.5 16.5 39.5 72-73 30.852611184596892 34.5 30.0 37.0 12.0 39.0 74-75 30.24607101104848 34.0 29.0 36.0 8.0 39.0 76-77 28.88895225131771 32.5 28.0 35.0 7.0 37.0 78-79 29.567485926405666 34.0 29.0 35.0 4.5 37.0 80-81 29.232456992522746 34.0 29.0 35.0 2.0 37.0 82-83 28.670807971591188 34.0 28.5 35.0 2.0 36.5 84-85 28.07309469054558 33.0 27.0 35.0 2.0 36.0 86-87 27.42190669074317 33.0 26.0 35.0 2.0 35.5 88-89 26.676626947754354 32.0 25.0 35.0 2.0 35.0 90-91 26.123653517895463 32.0 24.0 34.0 2.0 35.0 92-93 25.290569914250327 31.5 20.5 34.0 2.0 35.0 94-95 24.45577479321833 31.0 16.5 34.0 2.0 35.0 96-97 23.394093206803625 31.0 2.0 34.0 2.0 35.0 98-99 21.94121088711669 30.0 2.0 34.0 2.0 35.0 100-101 17.547282703154643 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1404546332025589 1101 2 0.11737507484092191 1101 3 0.11568087209548139 1101 4 0.062326151016286246 1101 5 0.09249242502115607 1101 6 0.061301728044846016 1101 7 0.02210217918349855 1101 8 0.03379497041412094 1101 9 -0.054071144873510946 1101 10-11 0.10241888113694131 1101 12-13 0.2202786731967592 1101 14-15 0.13688007949212988 1101 16-17 0.19486119992242124 1101 18-19 0.23102685967888448 1101 20-21 0.26463309081088227 1101 22-23 0.2136422395544031 1101 24-25 0.2226313318982207 1101 26-27 0.26731682784302535 1101 28-29 0.18607160014322233 1101 30-31 0.2708847175654512 1101 32-33 0.25120886364718586 1101 34-35 0.15138316849490963 1101 36-37 0.2735014031355405 1101 38-39 0.2891065589855941 1101 40-41 0.3146875527793114 1101 42-43 0.41919015197090204 1101 44-45 0.4671018301886747 1101 46-47 0.44837184607994374 1101 48-49 0.524042264422441 1101 50-51 0.35018273166950564 1101 52-53 0.11200179680088951 1101 54-55 0.040903821100627624 1101 56-57 0.16118709279801635 1101 58-59 0.27025806894326365 1101 60-61 0.2105914494650989 1101 62-63 0.1264340900494645 1101 64-65 0.09662233469999393 1101 66-67 -0.06640976541554267 1101 68-69 0.05887886359533212 1101 70-71 0.1369746931209299 1101 72-73 0.020999908440497705 1101 74-75 -0.18580217957533662 1101 76-77 -0.2325977917473132 1101 78-79 -0.10761580374256141 1101 80-81 -0.12849918944968763 1101 82-83 -0.038058351417333824 1101 84-85 -0.07324287501189275 1101 86-87 -0.2928428280610298 1101 88-89 -0.2787796690016826 1101 90-91 -0.5166356191303265 1101 92-93 -0.5876368635839988 1101 94-95 -0.6330531307942202 1101 96-97 -0.8568924775419653 1101 98-99 -0.7212675374709967 1101 100-101 -0.9937597006374475 1102 1 0.06263908376296001 1102 2 0.11362109522381303 1102 3 0.05236290921067166 1102 4 0.05608372944139006 1102 5 0.1205798700141969 1102 6 0.07155893493833076 1102 7 0.12561839582656376 1102 8 0.13858103983400838 1102 9 0.15060005611862692 1102 10-11 0.16770255264602696 1102 12-13 0.22130549855618398 1102 14-15 0.1463949135715481 1102 16-17 0.15013880437631855 1102 18-19 0.2517502114550254 1102 20-21 0.1660189339460061 1102 22-23 0.16210782692387227 1102 24-25 0.13694259167952083 1102 26-27 0.14966566711957086 1102 28-29 0.10615735015811367 1102 30-31 0.09047258932361046 1102 32-33 -0.032316535565918514 1102 34-35 0.06787364891420822 1102 36-37 0.08252845633317207 1102 38-39 -0.015180078735262725 1102 40-41 -0.08287267978825952 1102 42-43 -0.20513028175019343 1102 44-45 -0.3439716243304147 1102 46-47 -0.26774675751350685 1102 48-49 -0.3621265757689045 1102 50-51 -0.2545408548490329 1102 52-53 -0.3415740979171531 1102 54-55 -0.30020587177519786 1102 56-57 -0.32652655945701525 1102 58-59 -0.5255208831218212 1102 60-61 -0.47397415197166737 1102 62-63 -0.36592849719142606 1102 64-65 -0.4580783385446381 1102 66-67 -0.6175680724050849 1102 68-69 -0.46856942619415065 1102 70-71 -0.5281532889488929 1102 72-73 -0.5929239400919748 1102 74-75 -0.548644443907353 1102 76-77 -0.5807391411881184 1102 78-79 -0.6291617032158605 1102 80-81 -0.7650931118655997 1102 82-83 -0.8230650441645722 1102 84-85 -0.9520352807503087 1102 86-87 -1.0969399458214504 1102 88-89 -1.1968816732011547 1102 90-91 -1.3671591723361551 1102 92-93 -1.1411738359422188 1102 94-95 -1.0578713564696898 1102 96-97 -1.3171357466059384 1102 98-99 -1.1788653806026623 1102 100-101 -1.35771319402712 1103 1 0.10202497837325808 1103 2 0.1128555505767821 1103 3 0.1037871394918426 1103 4 0.0644654832649394 1103 5 0.02186050849679333 1103 6 0.06191824836917448 1103 7 0.026016612616786006 1103 8 -0.002880257167262812 1103 9 0.13969793229574634 1103 10-11 0.11020530760495717 1103 12-13 0.17042641620795962 1103 14-15 0.18733986448318518 1103 16-17 0.17542139637524912 1103 18-19 0.1369819619392345 1103 20-21 0.17360717611524734 1103 22-23 0.16661844603828513 1103 24-25 0.17064033094352027 1103 26-27 0.24583669163035182 1103 28-29 0.24094997688622044 1103 30-31 0.20666298203048683 1103 32-33 0.2807874683077287 1103 34-35 0.25634247613653827 1103 36-37 0.20436901934660057 1103 38-39 0.21507604896125798 1103 40-41 0.2768915625145496 1103 42-43 0.24570371945197422 1103 44-45 0.161629708067899 1103 46-47 0.15417979361185274 1103 48-49 0.21511036739546086 1103 50-51 -0.04484006008512864 1103 52-53 -0.06775497349318726 1103 54-55 -0.06973560863487904 1103 56-57 0.10295609265011052 1103 58-59 0.0720902335548601 1103 60-61 -0.018649985138033287 1103 62-63 -0.19317295388012923 1103 64-65 -0.14927666219386992 1103 66-67 -0.26231027503649074 1103 68-69 9.832686565829363E-4 1103 70-71 -0.37332042312374014 1103 72-73 -0.5735745278165432 1103 74-75 -0.5656129038018278 1103 76-77 -0.38905369071059326 1103 78-79 -0.5034500054932884 1103 80-81 -0.5027008057145288 1103 82-83 -0.5314576508566162 1103 84-85 -0.7236329064766167 1103 86-87 -0.9201225077662833 1103 88-89 -1.1989541536659907 1103 90-91 -1.43769572145791 1103 92-93 -1.2131316934190117 1103 94-95 -1.180465359007588 1103 96-97 -1.3712318696273762 1103 98-99 -1.2526435980607538 1103 100-101 -1.846135270431315 1104 1 0.053809135802580954 1104 2 0.10737074549520287 1104 3 0.08208144762485858 1104 4 0.047427246871862394 1104 5 3.291845461177445E-4 1104 6 -0.05082929179771867 1104 7 0.08432244148712442 1104 8 0.10327243930801444 1104 9 0.10354616993430454 1104 10-11 0.09775123486862469 1104 12-13 0.10398134210159071 1104 14-15 0.11911608194488821 1104 16-17 0.017113232220303587 1104 18-19 0.08586413904721013 1104 20-21 0.08244778515746276 1104 22-23 0.057995018463245174 1104 24-25 0.08942146807144269 1104 26-27 0.05693162873335922 1104 28-29 -0.07511678111602293 1104 30-31 -0.03647050283686326 1104 32-33 0.009706854283564326 1104 34-35 0.009382238248882402 1104 36-37 -0.0015742799434761423 1104 38-39 0.10555742895876818 1104 40-41 0.032602111773485376 1104 42-43 -0.08668979157057066 1104 44-45 -0.13079385028306945 1104 46-47 -0.09222776025537627 1104 48-49 -0.010324773967106182 1104 50-51 -0.09355098298959064 1104 52-53 -0.13887139521444425 1104 54-55 -0.16618986819397463 1104 56-57 -0.09428437938874623 1104 58-59 -0.14026264351576145 1104 60-61 -0.35883789770498 1104 62-63 -0.32890202973018035 1104 64-65 -0.3151176760187582 1104 66-67 -0.45906267259533706 1104 68-69 -0.37849892090625303 1104 70-71 -0.49325596926896154 1104 72-73 -0.6900421763406435 1104 74-75 -0.7317707379032079 1104 76-77 -0.6534211399570751 1104 78-79 -0.6369200827133703 1104 80-81 -0.6164696436769148 1104 82-83 -0.6013091267564761 1104 84-85 -0.7042679482003678 1104 86-87 -0.6052187214915286 1104 88-89 -0.7954379934966056 1104 90-91 -0.7301550875059846 1104 92-93 -0.806066679095931 1104 94-95 -0.5771959605769013 1104 96-97 -0.7316900524645895 1104 98-99 -0.7100192052347474 1104 100-101 -1.0841918465927307 1105 1 0.1728792651388673 1105 2 0.1329355824668852 1105 3 0.11594524080424407 1105 4 0.029521290943094414 1105 5 0.04548678103451209 1105 6 0.010164292770006966 1105 7 0.025193296046197133 1105 8 -0.009896079563930016 1105 9 0.061872285294640506 1105 10-11 0.07611126246819566 1105 12-13 0.099742312197165 1105 14-15 0.1351451202172811 1105 16-17 0.034070819900328786 1105 18-19 0.08574008778247588 1105 20-21 0.1239513745698062 1105 22-23 0.14947291678524266 1105 24-25 0.05348330285570313 1105 26-27 0.10449684740123644 1105 28-29 0.12867228769205497 1105 30-31 0.08541390193727239 1105 32-33 0.09017281725903104 1105 34-35 0.0801964256029919 1105 36-37 0.13683022994392502 1105 38-39 0.12208267229009095 1105 40-41 0.1662574401056247 1105 42-43 0.04416594194675838 1105 44-45 0.116187079587597 1105 46-47 0.17946726448146677 1105 48-49 0.21985785298358707 1105 50-51 0.1014987109194152 1105 52-53 -0.07087882360971065 1105 54-55 0.01093331691399868 1105 56-57 0.16366150783470346 1105 58-59 0.23631735853953018 1105 60-61 0.04276375826930945 1105 62-63 -0.07155437789582919 1105 64-65 -0.04678524799051331 1105 66-67 -0.23992281029958207 1105 68-69 -0.2190783650978645 1105 70-71 -0.09810424502978421 1105 72-73 -0.15130588437241244 1105 74-75 -0.05974466511668197 1105 76-77 -0.2546836735389988 1105 78-79 -0.2414490549229491 1105 80-81 -0.448567357903908 1105 82-83 -0.3925440607670794 1105 84-85 -0.45340032337837144 1105 86-87 -0.5680481179711165 1105 88-89 -0.5803712700648624 1105 90-91 -0.596408005279816 1105 92-93 -0.5319192562793056 1105 94-95 -0.5896766922591539 1105 96-97 -0.8069664151934255 1105 98-99 -1.0424134680472612 1105 100-101 -1.6026336452626673 1106 1 0.16248432885276998 1106 2 0.14535337097782985 1106 3 0.130838687007099 1106 4 0.07408023914985762 1106 5 0.1381066941986262 1106 6 0.0964076773142466 1106 7 0.10772977739446077 1106 8 0.08347272888330082 1106 9 0.1387416332547673 1106 10-11 0.11516798197287414 1106 12-13 0.05431917993943358 1106 14-15 0.0956182537595609 1106 16-17 0.09153408164115717 1106 18-19 0.16385641703949005 1106 20-21 0.2514692619914385 1106 22-23 0.2734269682701935 1106 24-25 0.286031024055994 1106 26-27 0.25681097236270034 1106 28-29 0.24626956058281735 1106 30-31 0.2776824894411263 1106 32-33 0.38163614965035464 1106 34-35 0.35304174597089144 1106 36-37 0.34548170461251004 1106 38-39 0.24851109691243778 1106 40-41 0.19624231447618712 1106 42-43 0.2533922547614722 1106 44-45 0.13860769411846974 1106 46-47 0.15461707565195582 1106 48-49 0.1256187588514237 1106 50-51 0.09477551314940058 1106 52-53 0.007442552661999002 1106 54-55 -0.1446647716688787 1106 56-57 0.021992165012797216 1106 58-59 0.031527897024780316 1106 60-61 -0.23279422009879625 1106 62-63 -0.06686655675720488 1106 64-65 -0.002454789918374445 1106 66-67 -0.23026199691966553 1106 68-69 -0.17256866028849416 1106 70-71 -0.5097919248674181 1106 72-73 -0.4721005740240436 1106 74-75 -0.2434804386754088 1106 76-77 -0.36105265540109244 1106 78-79 -0.28622027576356146 1106 80-81 -0.15041631356108454 1106 82-83 -0.11622225416960674 1106 84-85 -0.30929931885673767 1106 86-87 -0.47332924272705057 1106 88-89 -0.5670053197514306 1106 90-91 -0.577711796000191 1106 92-93 -0.5674445748488246 1106 94-95 -0.8839104876039272 1106 96-97 -0.8953845505340894 1106 98-99 -1.0551446878602313 1106 100-101 -1.3555661226545048 1107 1 -0.020516636069203287 1107 2 -0.005435838477026778 1107 3 0.06092646266619539 1107 4 -0.02653216389736457 1107 5 -0.012426603669794645 1107 6 0.027273262991947433 1107 7 -0.032099852849277966 1107 8 -0.01010368879204293 1107 9 0.017936107637126497 1107 10-11 0.07663572847704359 1107 12-13 0.031834655537728906 1107 14-15 -0.01904179547678808 1107 16-17 -0.04609511196962757 1107 18-19 -0.005753376803433241 1107 20-21 0.050794133258413865 1107 22-23 -0.020823282747699068 1107 24-25 -0.01669626227941734 1107 26-27 -0.05123806593940827 1107 28-29 -0.02603663172912718 1107 30-31 -0.12482037768981513 1107 32-33 -0.0030814379525807567 1107 34-35 0.06257763346881262 1107 36-37 0.06721686001331051 1107 38-39 0.008784407032379704 1107 40-41 -0.045374637982405375 1107 42-43 0.03311081534659621 1107 44-45 -0.04909150815128527 1107 46-47 -0.1147914720828922 1107 48-49 -0.11479966954629361 1107 50-51 -0.13798384705436462 1107 52-53 -0.14764576993974288 1107 54-55 -0.06156403289329404 1107 56-57 -0.03470016765729156 1107 58-59 -0.08474577695594832 1107 60-61 -0.12212138276760243 1107 62-63 -0.06673802523488348 1107 64-65 -0.3291112489080348 1107 66-67 -0.36676362508156757 1107 68-69 -0.3312003076567187 1107 70-71 -0.47504134798685627 1107 72-73 -0.6042761737034752 1107 74-75 -0.6615983744793148 1107 76-77 -0.6949535136980352 1107 78-79 -0.7347852134005883 1107 80-81 -0.6939018523974667 1107 82-83 -0.7050077421115333 1107 84-85 -0.8497267286284753 1107 86-87 -0.9078006197789499 1107 88-89 -1.000591657239223 1107 90-91 -1.0597762511762276 1107 92-93 -1.1857986459444625 1107 94-95 -1.4456653502119323 1107 96-97 -1.5126378275841468 1107 98-99 -1.450644098313628 1107 100-101 -1.7723365644920506 1108 1 -0.0789235675867559 1108 2 -0.08698062170158494 1108 3 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3.420007833559403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 19.0 5 48.0 6 108.0 7 229.0 8 610.0 9 1170.0 10 1927.0 11 3307.0 12 5112.0 13 7765.0 14 10573.0 15 14369.0 16 19088.0 17 26290.0 18 26885.0 19 21880.0 20 20792.0 21 21975.0 22 23561.0 23 25911.0 24 28901.0 25 33275.0 26 38362.0 27 45156.0 28 54411.0 29 65651.0 30 79460.0 31 96289.0 32 120198.0 33 154950.0 34 209097.0 35 296098.0 36 427216.0 37 538067.0 38 298980.0 39 15222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.686060966233676 18.84873273360747 15.14052255412518 23.324683746033674 2 15.722184549389207 19.660900588489095 40.04144784199318 24.575467020128517 3 19.34332705752338 23.43650911273221 29.666211075798245 27.553952753946163 4 13.125504490745179 16.681400418741042 36.323992280879956 33.86910280963382 5 14.0529280424806 36.80875167129054 34.17614432632081 14.962175959908055 6 32.902422924253244 35.76532001217756 16.85852663255389 14.473730431015303 7 29.168427581134704 32.468079423188456 19.933954272938998 18.429538722737842 8 26.544381447919925 35.12447149696939 19.045831343728675 19.285315711382005 9 26.07518235755803 17.060727307987143 20.043835335744642 36.82025499871019 10-11 25.252556298951134 26.38749997713098 26.720491189939093 21.63945253397879 12-13 25.960913428738753 23.570258844571228 27.43176883008679 23.03705889660323 14-15 22.730652352490253 25.201055268015754 25.716705909903382 26.35158646959061 16-17 22.385407913304597 28.438402951320374 26.506154591212162 22.670034544162867 18-19 22.291658662509995 26.851356864639193 28.784191470404746 22.07279300244607 20-21 22.725328444851815 25.95966636845466 29.30659304672049 22.008412139973032 22-23 22.578966722832977 25.77640319727182 29.15889577398823 22.485734305906977 24-25 22.385998281290565 26.075204240824966 29.186262253397487 22.35253522448698 26-27 22.178061940650412 26.467678242088983 29.009352525489295 22.34490729177131 28-29 22.08052157580031 26.567904550486855 29.03256024828788 22.319013625424954 30-31 22.343966347490845 26.196982515097673 28.96183941289603 22.497211724515452 32-33 22.185271985817547 26.246882952701377 28.980499130062515 22.587345931418557 34-35 22.64914204454697 26.390238038473836 28.76603022993656 22.194589687042633 36-37 22.753820990658376 26.06689523698009 28.739484728844133 22.439799043517397 38-39 22.27084422312266 26.375900677437137 28.741670626174244 22.61158447326596 40-41 22.719592724856376 26.182587728871347 28.732265369190063 22.36555417708221 42-43 22.50094863745656 26.21563727762822 28.604447270942934 22.678966813972288 44-45 22.443307701736764 26.448807243441625 28.634408543133716 22.4734765116879 46-47 22.671766646468726 26.74188935627117 27.998662252392286 22.587681744867822 48-49 22.680090788641248 26.6303590161661 28.0161737849445 22.673376410248153 50-51 22.659955489755397 26.790710370916266 27.93063588468319 22.618698254645146 52-53 22.861257488546823 26.790787982756605 27.750917434465894 22.59703709423068 54-55 22.634610286235368 26.754900753576454 27.83064134411678 22.779847616071393 56-57 22.598110846766772 26.995180686736585 27.73999861667521 22.66670984982143 58-59 22.736517997017593 26.73231942183219 27.659157089917475 22.87200549123275 60-61 22.4962612469048 26.8858336477167 27.882700303674206 22.735204801704292 62-63 22.432843558213168 26.864077202665964 28.0103401917221 22.692739047398767 64-65 22.344519207244716 26.840882229101354 27.892459411887522 22.92213915176641 66-67 22.30156445869539 26.91003991976159 27.70137229784091 23.087023323702113 68-69 22.460811610604647 26.666497079377464 27.927677487433765 22.945013822584123 70-71 22.471242126065295 26.675138723061654 27.68818074940188 23.16543840147117 72-73 22.528817186280452 26.797302870173823 27.511159672881348 23.162720270664376 74-75 22.427962831283878 27.11657730448126 27.316887421944564 23.138572442290304 76-77 22.511911650122656 26.88329236216101 27.50836349902229 23.096432488694045 78-79 22.575682990610424 26.988344920094352 27.512576469877775 22.923395619417448 80-81 22.64888321918604 27.124862336187157 27.246026618449797 22.98022782617701 82-83 22.558346214565024 27.09892090478406 27.36775217962153 22.974980701029388 84-85 22.630458177570137 27.079766921103015 27.16518697347861 23.124587927848236 86-87 22.653556205559898 26.974232633028176 27.230462368305037 23.141748793106885 88-89 22.59881846768243 27.1746028376685 26.969086336327543 23.257492358321525 90-91 22.66597248401489 27.080717948342564 27.078302339114934 23.175007228527615 92-93 22.609166821601477 26.824979541285614 27.163627992498718 23.402225644614198 94-95 22.46380033576235 26.809333644393824 27.186595122754532 23.540270897089297 96-97 22.586687560291182 26.65102694457822 27.147610383184777 23.614675111945814 98-99 22.460898118286554 26.599635740735522 26.937986269101742 24.001479871876178 100-101 22.947387642489858 26.198025428748075 26.791121363913895 24.063465564848173 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 787.0 1 517.5 2 387.0 3 512.5 4 402.0 5 400.0 6 831.5 7 1849.5 8 3073.5 9 3550.5 10 3038.5 11 2445.5 12 2328.0 13 2499.0 14 2729.0 15 2877.0 16 3014.0 17 3283.0 18 3587.0 19 3850.5 20 4359.0 21 5092.0 22 6152.5 23 7626.0 24 9349.5 25 11813.0 26 15287.0 27 19030.5 28 22042.0 29 25480.0 30 30012.0 31 35140.0 32 41597.5 33 46056.0 34 49306.5 35 53576.5 36 59265.0 37 65303.5 38 68883.5 39 71779.0 40 75057.5 41 79309.0 42 83260.5 43 87039.5 44 91095.0 45 94838.0 46 97402.0 47 100056.0 48 102278.5 49 102560.5 50 101923.0 51 99831.0 52 97255.5 53 96984.0 54 99579.0 55 95004.0 56 85080.5 57 77904.5 58 72449.0 59 64354.5 60 52778.0 61 43631.0 62 36299.5 63 28182.0 64 21494.0 65 16564.5 66 11759.0 67 9031.0 68 6727.5 69 4399.5 70 3153.5 71 2118.0 72 1488.0 73 1089.0 74 804.5 75 579.0 76 364.5 77 208.5 78 123.0 79 74.5 80 40.5 81 22.0 82 16.5 83 10.5 84 6.0 85 2.5 86 1.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009586692792234047 2 0.013318916703714477 3 0.008781703321130424 4 3.65904305047101E-5 5 0.0013538459286742738 6 0.002158835399777896 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.866942558512673E-4 14-15 0.0 16-17 0.002799167933610323 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003311433960676264 26-27 0.017728063579532046 28-29 0.005836173665501262 30-31 0.0030370057318909387 32-33 0.0 34-35 0.009751349729505242 36-37 9.147607626177525E-4 38-39 0.0029821200861338735 40-41 0.0022137210455349613 42-43 0.0032199578844144894 44-45 0.0 46-47 1.0977129151413032E-4 48-49 5.122660270659414E-4 50-51 0.0036956334809757204 52-53 0.007263200455184956 54-55 0.006824115289128435 56-57 0.014928895645921722 58-59 0.021935963087573708 60-61 0.0033663196064333293 62-63 0.018679414772654508 64-65 0.01959417553527226 66-67 0.012751765030891472 68-69 0.030443238179918806 70-71 0.01619126549833422 72-73 0.004537213382584053 74-75 0.016172970283081865 76-77 0.019978375055571717 78-79 0.024515588438155766 80-81 0.011032014797170095 82-83 0.03454136639644634 84-85 0.021057792755460665 86-87 0.030607895117190004 88-89 0.020893135818189468 90-91 0.026528062115914828 92-93 0.022795838204434395 94-95 0.013446983210480963 96-97 0.01401413488330397 98-99 0.01046486312434709 100-101 0.009531807146476983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2732955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.12481239299807 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95032007335894 40.56672012765709 2 14.153412565280457 15.321015996330125 3 4.709033741168336 7.64626703379102 4 2.1048773734024175 4.557043717826895 5 1.092616776648576 2.9568839026773217 6 0.6827097726290018 2.217092301744665 7 0.4420806136127626 1.6749271192060573 8 0.29884421327506877 1.2939909582596958 9 0.21868791242800797 1.065279800950409 >10 1.2071383692141744 11.975673062915742 >50 0.09077283670735345 3.38089628471376 >100 0.0446973895870043 4.2561946434053315 >500 0.003521617743236135 1.3091395041873903 >1k 0.0010835746902068727 0.8693140831829482 >5k 1.3544683627585909E-4 0.5271746982769928 >10k+ 6.772341813792954E-5 0.3823867648745368 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10432 0.3817113710251358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8872 0.32463029943778804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5510 0.20161327208095264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.65904305047101E-5 0.0 14-15 0.0 0.0 0.0 7.31808610094202E-5 5.488564575706515E-5 16-17 0.0 0.0 0.0 7.31808610094202E-5 7.31808610094202E-5 18-19 0.0 0.0 0.0 1.2806650676648536E-4 7.31808610094202E-5 20-21 0.0 0.0 0.0 1.6465693727119545E-4 7.31808610094202E-5 22-23 0.0 0.0 0.0 4.024947355518112E-4 9.147607626177526E-5 24-25 0.0 0.0 0.0 7.683990405989122E-4 1.0977129151413032E-4 26-27 0.0 0.0 0.0 0.0011708937761507232 1.0977129151413032E-4 28-29 0.0 0.0 0.0 0.003384614821685684 1.0977129151413032E-4 30-31 0.0 0.0 0.0 0.011306443025955421 1.2806650676648536E-4 32-33 0.0 0.0 0.0 0.028449059717412108 2.1954258302826063E-4 34-35 0.0 0.0 0.0 0.054025770640204474 2.561330135329707E-4 36-37 0.0 0.0 0.0 0.09830019155090369 2.561330135329707E-4 38-39 0.0 0.0 0.0 0.16222367364263224 2.561330135329707E-4 40-41 0.0 0.0 0.0 0.22976960835432708 2.561330135329707E-4 42-43 0.0 0.0 0.0 0.2971325909134984 2.561330135329707E-4 44-45 0.0 0.0 0.0 0.37867436529324483 2.561330135329707E-4 46-47 0.0 0.0 0.0 0.4759317295747643 3.2931387454239095E-4 48-49 0.0 0.0 0.0 0.5758053096373705 3.2931387454239095E-4 50-51 0.0 0.0 0.0 0.6808198451858885 3.2931387454239095E-4 52-53 0.0 0.0 0.0 0.7812971673518225 3.2931387454239095E-4 54-55 0.0 0.0 0.0 0.8912331158032241 3.2931387454239095E-4 56-57 0.0 0.0 0.0 1.015677169949743 3.2931387454239095E-4 58-59 0.0 0.0 0.0 1.1464696637888294 3.47609089794746E-4 60-61 0.0 0.0 0.0 1.285732842289756 3.6590430504710104E-4 62-63 0.0 0.0 0.0 1.4278134839395453 3.6590430504710104E-4 64-65 0.0 0.0 0.0 1.574193501173638 3.6590430504710104E-4 66-67 0.0 0.0 0.0 1.736819669551822 3.6590430504710104E-4 68-69 0.0 0.0 0.0 1.9157102842893499 4.024947355518111E-4 70-71 0.0 0.0 0.0 2.102723974598923 4.024947355518111E-4 72-73 0.0 0.0 0.0 2.2907621969626284 5.122660270659414E-4 74-75 0.0 0.0 0.0 2.4814715207531775 5.122660270659414E-4 76-77 0.0 0.0 0.0 2.690457764580829 5.122660270659414E-4 78-79 0.0 0.0 0.0 2.916147539933881 5.122660270659414E-4 80-81 0.0 0.0 0.0 3.159620264512222 5.122660270659414E-4 82-83 0.0 0.0 0.0 3.4068800986478007 6.95218179589492E-4 84-85 0.0 0.0 0.0 3.65693910071699 6.95218179589492E-4 86-87 0.0 0.0 0.0 3.912065877411081 6.95218179589492E-4 88-89 0.0 0.0 0.0 4.191250862162018 6.95218179589492E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5430 0.0 48.993988 1 GTATCAA 9415 0.0 41.07321 1 ATCAACG 11740 0.0 32.9306 3 TATCAAC 11875 0.0 32.600998 2 TCAACGC 12090 0.0 31.935652 4 CAACGCA 12295 0.0 31.364548 5 AACGCAG 12685 0.0 30.437685 6 ACGCAGA 14435 0.0 26.648443 7 CGCAGAG 14435 0.0 26.549746 8 GCAGAGT 15770 0.0 23.850477 9 GTACATG 8820 0.0 23.430162 1 GAGTACT 9455 0.0 23.180017 12-13 TACATGG 8945 0.0 22.569164 2 TAAGGTG 1570 0.0 21.476833 5 ACATGGG 9030 0.0 21.248924 3 AGAGTAC 13960 0.0 21.091616 10-11 GTACTTT 10360 0.0 20.971378 14-15 TGGTATC 2765 0.0 20.443665 2 CAGAGTA 15320 0.0 20.31971 10-11 AGTACTT 10075 0.0 20.033068 12-13 >>END_MODULE