FastQCFastQC Report
Thu 26 May 2016
SRR938175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626684
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156710.4321027142149689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123370.34017300652607174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79860.22020115345036956No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45910.1265894685062167No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45510.12548653260113096No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41700.1149810681051892No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40650.11208586135433912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68600.053.418481
GTATCAA120850.041.891231
TATCAAC155000.032.5633962
ATCAACG155100.032.4811173
TCAACGC158150.031.8547024
CAACGCA159900.031.476355
AACGCAG167250.030.1440936
ACGCAGA189450.026.5542777
CGCAGAG190400.026.2471378
GTACATG123850.023.5781561
TGGTATC31950.023.5029892
GTGGTAT33850.023.3993511
GCAGAGT212500.022.913229
TACATGG124300.022.6735972
TAAGGTG22350.022.1152945
GAGTACT128850.021.84304212-13
AGAGTAC184050.021.37025510-11
ACATGGG125350.021.3462183
AGGTAAG20300.021.0709112
CAGAGTA196850.020.81320210-11
Produced by FastQC (version 0.11.5)