FastQCFastQC Report
Thu 26 May 2016
SRR938175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626684
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153540.4233619471671643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125760.34676304855895906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82450.22734266343579973No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46510.12824387236384532No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45970.12675490889197955No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42950.11842774280858216No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41350.11401599918823917No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39870.10993513633942191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.053.547691
GTATCAA124950.042.0607451
ATCAACG158750.032.800423
TATCAAC159050.032.7738762
TCAACGC160050.032.53224
CAACGCA163550.031.8937135
AACGCAG169900.030.7220426
ACGCAGA193000.026.872697
CGCAGAG194250.026.5286088
TAAGGTA11250.023.6695774
AGGTAAG18450.023.2004852
GTACATG122400.023.1587851
GCAGAGT218150.023.1432239
TGGTATC32650.022.7243982
TACATGG121650.022.5978282
GTGTAAG13100.022.5097851
GAGTACT133850.022.30173512-13
GTGGTAT34900.021.8044681
ACATGGG119750.021.5627063
TAAGGTG22850.021.4338425
Produced by FastQC (version 0.11.5)