##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938175_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3626684 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4880775386 33.0 31.0 34.0 31.0 34.0 2 32.714223516578784 34.0 31.0 34.0 31.0 34.0 3 32.77978478411684 34.0 31.0 34.0 31.0 34.0 4 36.24049131382828 37.0 35.0 37.0 35.0 37.0 5 36.16318929358058 37.0 35.0 37.0 35.0 37.0 6 36.21478711682628 37.0 37.0 37.0 35.0 37.0 7 36.18282458576485 37.0 37.0 37.0 35.0 37.0 8 36.17564199141695 37.0 37.0 37.0 35.0 37.0 9 37.864948807229965 39.0 38.0 39.0 35.0 39.0 10-11 37.840864823072536 39.0 38.0 39.0 35.0 39.0 12-13 37.75898892762645 39.0 38.0 39.0 35.0 39.0 14-15 39.017825650098 40.0 38.0 41.0 36.0 41.0 16-17 38.928321574198364 40.0 38.0 41.0 35.5 41.0 18-19 39.16560596401561 40.0 39.0 41.0 36.0 41.0 20-21 39.12936004349979 40.0 39.0 41.0 36.0 41.0 22-23 39.021795530021365 40.0 38.5 41.0 35.0 41.0 24-25 38.91249733916713 40.0 38.0 41.0 35.0 41.0 26-27 38.75329874342512 40.0 38.0 41.0 35.0 41.0 28-29 38.62788776193349 40.0 38.0 41.0 34.5 41.0 30-31 38.38730862683377 40.0 38.0 41.0 34.0 41.0 32-33 38.16347495398 40.0 38.0 41.0 34.0 41.0 34-35 38.076839200768525 40.0 38.0 41.0 33.0 41.0 36-37 38.0261295166604 40.0 38.0 41.0 33.0 41.0 38-39 37.84040200359337 40.0 37.0 41.0 33.0 41.0 40-41 37.6180847297421 40.0 37.0 41.0 33.0 41.0 42-43 37.36425202747193 40.0 37.0 41.0 32.0 41.0 44-45 37.09474453798566 40.0 36.0 41.0 31.5 41.0 46-47 36.73082490782213 39.0 36.0 41.0 31.0 41.0 48-49 36.31232745946434 39.0 35.0 41.0 30.0 41.0 50-51 35.91693293377642 39.0 35.0 40.0 29.0 41.0 52-53 35.370965460459196 38.0 34.5 40.0 27.5 41.0 54-55 34.971170220509975 38.0 34.0 40.0 26.5 41.0 56-57 34.56704140752269 38.0 34.0 40.0 26.0 41.0 58-59 34.0363461498162 37.0 33.0 40.0 24.5 41.0 60-61 33.522822639082975 36.5 33.0 39.0 23.0 41.0 62-63 32.93908815876983 36.0 32.0 39.0 21.5 40.0 64-65 32.34895637447321 35.0 31.0 38.5 19.5 40.0 66-67 31.76209107272649 35.0 31.0 38.0 17.5 40.0 68-69 31.115458363618117 35.0 30.0 37.5 11.5 39.5 70-71 30.478799641766418 34.0 29.0 37.0 7.5 39.0 72-73 29.804703966488397 34.0 29.0 36.0 6.5 39.0 74-75 29.148201359699385 34.0 28.0 35.5 2.0 38.5 76-77 27.87733036570046 31.5 26.5 34.5 2.0 36.0 78-79 28.534854704738542 33.5 28.0 35.0 2.0 37.0 80-81 28.26981479500282 33.5 27.0 35.0 2.0 36.5 82-83 27.727866420123725 33.0 26.0 35.0 2.0 36.0 84-85 27.0838919244136 33.0 25.5 35.0 2.0 35.5 86-87 26.37455717123411 32.0 24.5 35.0 2.0 35.0 88-89 25.561382519127665 32.0 21.5 34.0 2.0 35.0 90-91 24.901001024627455 31.0 19.0 34.0 2.0 35.0 92-93 23.959832590873646 31.0 10.0 34.0 2.0 35.0 94-95 23.052670704147367 30.5 2.0 34.0 2.0 35.0 96-97 21.987572393955467 30.0 2.0 34.0 2.0 35.0 98-99 20.542432977342386 29.0 2.0 34.0 2.0 35.0 100-101 16.67538997056264 20.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1488668026127442 1101 2 0.10428786671390355 1101 3 0.11027976017560093 1101 4 0.014925396869735152 1101 5 -0.03919785231035178 1101 6 0.004576717116457019 1101 7 0.025478310614502675 1101 8 0.054229094148482204 1101 9 -0.021662744180979132 1101 10-11 0.06159134601944771 1101 12-13 0.19618705637565625 1101 14-15 0.2681126774085385 1101 16-17 0.3319723618134063 1101 18-19 0.24865677635724381 1101 20-21 0.16870919734393652 1101 22-23 0.1825245786289571 1101 24-25 0.17178206045110755 1101 26-27 0.23024297684283113 1101 28-29 0.23755910686236348 1101 30-31 0.29049214145117475 1101 32-33 0.2624591688003264 1101 34-35 0.262265622544696 1101 36-37 0.30279733689231847 1101 38-39 0.32902438398562595 1101 40-41 0.3687125970938112 1101 42-43 0.5153622629781651 1101 44-45 0.48842208496039063 1101 46-47 0.5315998852046491 1101 48-49 0.6808372108524665 1101 50-51 0.6780200333308173 1101 52-53 0.47995792014405225 1101 54-55 0.6393302868736654 1101 56-57 0.7705996432646529 1101 58-59 0.9460730988684816 1101 60-61 0.9706699424172598 1101 62-63 0.9841151583519476 1101 64-65 1.0913993401893478 1101 66-67 1.1866104048401027 1101 68-69 1.3953088490743575 1101 70-71 1.5515692681223463 1101 72-73 1.646652644009631 1101 74-75 1.7823215018670417 1101 76-77 1.8185732208482328 1101 78-79 1.8913905434708695 1101 80-81 2.103043434817767 1101 82-83 2.2574069554768705 1101 84-85 2.5443735246730697 1101 86-87 2.715845346382242 1101 88-89 2.9329114278391835 1101 90-91 3.3027761774580107 1101 92-93 3.6847441575112967 1101 94-95 3.889855555372627 1101 96-97 4.160457108268222 1101 98-99 4.414421693596363 1101 100-101 4.334820928045627 1102 1 0.15426491327401237 1102 2 0.16890595038461953 1102 3 0.20536882900151454 1102 4 0.12408059255125181 1102 5 0.023889866987900632 1102 6 0.07481153493021964 1102 7 0.03219221074945722 1102 8 0.1386595934737187 1102 9 0.20693239751942372 1102 10-11 0.21214870094522098 1102 12-13 0.2674360442299033 1102 14-15 0.32697570035050916 1102 16-17 0.32964510135457203 1102 18-19 0.2788187196770764 1102 20-21 0.23725575604838411 1102 22-23 0.26826749360871105 1102 24-25 0.3317368512743144 1102 26-27 0.3683495895283926 1102 28-29 0.21948488284076717 1102 30-31 0.3411183222878762 1102 32-33 0.2993842700148974 1102 34-35 0.3411374174164834 1102 36-37 0.36534591988827003 1102 38-39 0.436404276023417 1102 40-41 0.5914049047861241 1102 42-43 0.5763953264059651 1102 44-45 0.48303814433960923 1102 46-47 0.5710438798065312 1102 48-49 0.7319627169253451 1102 50-51 0.8179822465561841 1102 52-53 0.9104592696717262 1102 54-55 1.0010981681152273 1102 56-57 1.1145753517666819 1102 58-59 1.2680258654001975 1102 60-61 1.0826476752107865 1102 62-63 1.3863472771103815 1102 64-65 1.476823766640095 1102 66-67 1.513330377849556 1102 68-69 1.6732494698570868 1102 70-71 1.7826650845865935 1102 72-73 2.0952140475184002 1102 74-75 2.1329712994378944 1102 76-77 2.1920772694312305 1102 78-79 2.202227250623366 1102 80-81 2.4763268356274857 1102 82-83 2.5965128933985966 1102 84-85 2.7031191387081606 1102 86-87 2.7977603261393256 1102 88-89 3.0308587962602367 1102 90-91 3.4313105904134744 1102 92-93 3.4393480711415236 1102 94-95 3.576798200446852 1102 96-97 3.7322249895097848 1102 98-99 4.1797071186975785 1102 100-101 3.849545624401898 1103 1 0.16770061384983137 1103 2 0.17413563688494094 1103 3 0.17000542529070373 1103 4 0.09940880752245818 1103 5 0.036196968715344724 1103 6 0.09948076077795065 1103 7 0.07878530096058967 1103 8 0.023458697760347036 1103 9 0.14645447045608506 1103 10-11 0.18300026844362804 1103 12-13 0.21325722145319048 1103 14-15 0.3454161993908187 1103 16-17 0.33559743660728714 1103 18-19 0.2809460389349141 1103 20-21 0.21321608874193032 1103 22-23 0.3307265467117233 1103 24-25 0.3585669856313203 1103 26-27 0.2670117776281984 1103 28-29 0.2673089391427581 1103 30-31 0.36429362619063 1103 32-33 0.44583692516524565 1103 34-35 0.5182698550364435 1103 36-37 0.49566549762339207 1103 38-39 0.4444685754475941 1103 40-41 0.6233018977483695 1103 42-43 0.6513815708077573 1103 44-45 0.6607083298802294 1103 46-47 0.6965851466011586 1103 48-49 0.8348501129586126 1103 50-51 0.9792004167417332 1103 52-53 1.1443111570677544 1103 54-55 1.227419345338518 1103 56-57 1.144055198215817 1103 58-59 1.3305460189510612 1103 60-61 1.2208137020821823 1103 62-63 1.300296733284405 1103 64-65 1.436002589416809 1103 66-67 1.54520593767041 1103 68-69 1.6728035325570225 1103 70-71 1.696176920799001 1103 72-73 1.9307469969745767 1103 74-75 2.0208123103144153 1103 76-77 1.950228581011526 1103 78-79 2.15284145407826 1103 80-81 2.17024622142403 1103 82-83 2.4258107180946915 1103 84-85 2.6452422992967684 1103 86-87 2.928370051027361 1103 88-89 3.0847954565289513 1103 90-91 3.2903688117826384 1103 92-93 3.4752600992925125 1103 94-95 3.448149128149989 1103 96-97 3.5548481500983975 1103 98-99 3.9078168435856107 1103 100-101 3.6847196487141183 1104 1 0.15634824660735092 1104 2 0.14440595038461623 1104 3 0.1216604956681806 1104 4 0.11914309255124778 1104 5 -0.010464299678766054 1104 6 0.04008236826355471 1104 7 0.027504710749454375 1104 8 0.07388876014038459 1104 9 0.0747032308527551 1104 10-11 0.16612786761189113 1104 12-13 0.1944360442299029 1104 14-15 0.2858298670171777 1104 16-17 0.21721801802123508 1104 18-19 0.19647496967708378 1104 20-21 0.22488075604838542 1104 22-23 0.2904237436087058 1104 24-25 0.23674726794098433 1104 26-27 0.35650583952839554 1104 28-29 0.345609882840769 1104 30-31 0.33050373895454044 1104 32-33 0.4271030200148971 1104 34-35 0.47422075074981507 1104 36-37 0.466220919888265 1104 38-39 0.4878521926900774 1104 40-41 0.5349465714527852 1104 42-43 0.48865574307263415 1104 44-45 0.6146735610062706 1104 46-47 0.645439713139865 1104 48-49 0.6077543835920025 1104 50-51 0.7127009965561868 1104 52-53 0.9113551030050573 1104 54-55 1.0090669181152307 1104 56-57 0.9246691017666748 1104 58-59 1.1243591987335293 1104 60-61 1.1423039252107827 1104 62-63 1.2768785271103766 1104 64-65 1.3505529333067656 1104 66-67 1.3773199611828844 1104 68-69 1.5631036365237527 1104 70-71 1.6343630012532593 1104 72-73 1.7560057141850685 1104 74-75 1.713242132771228 1104 76-77 1.866098102764564 1104 78-79 1.8983730839566988 1104 80-81 2.0089518356274887 1104 82-83 2.1909920600652626 1104 84-85 2.2274628887081604 1104 86-87 2.4716249094726592 1104 88-89 2.6850358795935705 1104 90-91 3.0139772570801426 1104 92-93 3.257327237808191 1104 94-95 3.355423200446854 1104 96-97 3.707172906176453 1104 98-99 3.9075300353642426 1104 100-101 3.8833893744018972 1105 1 0.14906751197466406 1105 2 0.1625581242976608 1105 3 0.139266380225898 1105 4 0.11769678445529763 1105 5 -0.012156110023596511 1105 6 -0.01416097881291023 1105 7 0.0998458839128773 1105 8 0.03421531561264857 1105 9 0.143349688873748 1105 10-11 0.10013320869135356 1105 12-13 0.17105898276063414 1105 14-15 0.26358614512812295 1105 16-17 0.2814414531786582 1105 18-19 0.2486463058839803 1105 20-21 0.14885095844719132 1105 22-23 0.2500688429340485 1105 24-25 0.2525556918540275 1105 26-27 0.2665220033214979 1105 28-29 0.28341441807315704 1105 30-31 0.29362756766519027 1105 32-33 0.3485589326835665 1105 34-35 0.42825186019509687 1105 36-37 0.5056090383290481 1105 38-39 0.5344659951638349 1105 40-41 0.5051405369700248 1105 42-43 0.41319917448192456 1105 44-45 0.6232228020107655 1105 46-47 0.5566095969479576 1105 48-49 0.6646218873401324 1105 50-51 0.6239882435576902 1105 52-53 0.6949837574278419 1105 54-55 0.869104165116731 1105 56-57 0.8713864462194536 1105 58-59 0.9200563501578145 1105 60-61 1.0862181399783992 1105 62-63 1.0587265874552116 1105 64-65 1.1631583493487412 1105 66-67 1.2065490185292163 1105 68-69 1.4762384753543394 1105 70-71 1.641192320968397 1105 72-73 1.7072960065388934 1105 74-75 1.7196921889931183 1105 76-77 1.5936482339489721 1105 78-79 1.8353956664154687 1105 80-81 2.0285967006949512 1105 82-83 2.223273263213688 1105 84-85 2.409785555499763 1105 86-87 2.49201869695392 1105 88-89 2.507261095110813 1105 90-91 2.6944870022075804 1105 92-93 2.8921834034753573 1105 94-95 3.1006265362789343 1105 96-97 3.509446378815131 1105 98-99 3.4548737853642457 1105 100-101 3.441289252587808 1106 1 0.1429112014836207 1106 2 0.09398018619248205 1106 3 0.11049692216017348 1106 4 0.08457840914513781 1106 5 -0.04035467449681818 1106 6 0.03819144759397375 1106 7 0.0176725600944323 1106 8 0.09558681326993934 1106 9 0.15464273230836056 1106 10-11 0.05037140836880383 1106 12-13 0.10296224510326368 1106 14-15 0.027561580990969503 1106 16-17 0.04226737209692999 1106 18-19 0.043702271351023114 1106 20-21 0.08321499913427033 1106 22-23 -0.0076400755324854686 1106 24-25 -0.017768971141990164 1106 26-27 -0.014996844241622398 1106 28-29 0.050199584441934064 1106 30-31 0.10207465416561234 1106 32-33 0.22481294541518793 1106 34-35 0.07576914958533365 1106 36-37 0.09982263022305915 1106 38-39 0.07857385389822724 1106 40-41 0.0807717170131994 1106 42-43 0.14986184751221998 1106 44-45 0.16837511668313 1106 46-47 0.3517993383218183 1106 48-49 0.49475310993844346 1106 50-51 0.6473359583465808 1106 52-53 0.5972343788420247 1106 54-55 0.6662510065431775 1106 56-57 0.7381168364828383 1106 58-59 0.8447813239154769 1106 60-61 0.721325258908017 1106 62-63 0.7926020078236249 1106 64-65 0.84906321351054 1106 66-67 1.056494133308064 1106 68-69 0.9610908090128341 1106 70-71 0.9902793495065332 1106 72-73 1.2949403939521709 1106 74-75 1.4821408773127125 1106 76-77 1.451734474671408 1106 78-79 1.5713538881779527 1106 80-81 1.402533531406231 1106 82-83 1.9268367653054348 1106 84-85 1.9590674647634714 1106 86-87 2.116183906925354 1106 88-89 2.103416292621226 1106 90-91 2.165214520544481 1106 92-93 2.1590635005447254 1106 94-95 2.2619561334890648 1106 96-97 2.7388334320134256 1106 98-99 2.975252242423924 1106 100-101 2.8070026840816666 1107 1 0.21133925380159013 1107 2 0.19266134606807128 1107 3 0.19988441653148925 1107 4 0.11382159974549211 1107 5 0.04073399168813552 1107 6 0.07515206250815964 1107 7 0.09640084384297865 1107 8 0.12282985726268691 1107 9 0.20248875243549236 1107 10-11 0.2561031373960603 1107 12-13 0.26038928163997355 1107 14-15 0.3858186260099856 1107 16-17 0.32426500543130743 1107 18-19 0.2830992952166511 1107 20-21 0.1847233819476699 1107 22-23 0.26600490368065266 1107 24-25 0.32668769060285285 1107 26-27 0.35652944564350264 1107 28-29 0.2870891993875304 1107 30-31 0.41130297456605547 1107 32-33 0.39673319087820147 1107 34-35 0.3890319018289574 1107 36-37 0.41702577600337776 1107 38-39 0.414642885136125 1107 40-41 0.5741507081434349 1107 42-43 0.5586411297632807 1107 44-45 0.6465429405026768 1107 46-47 0.6531757742909505 1107 48-49 0.6479027648869717 1107 50-51 0.650226850872734 1107 52-53 0.7041990778252014 1107 54-55 0.736781621352641 1107 56-57 0.7806257114789119 1107 58-59 0.9414095584457627 1107 60-61 0.9379390421172573 1107 62-63 0.9236782123621836 1107 64-65 1.0276235268319382 1107 66-67 1.1181709054274904 1107 68-69 1.3076787264518153 1107 70-71 1.4994540534115295 1107 72-73 1.5469454623865069 1107 74-75 1.495524057231659 1107 76-77 1.474378228663845 1107 78-79 1.5087260515825989 1107 80-81 1.6232908644044635 1107 82-83 1.9806076176192207 1107 84-85 2.1128349660462895 1107 86-87 2.2972015731417237 1107 88-89 2.4267748634065214 1107 90-91 2.5224664657132365 1107 92-93 2.840018334930491 1107 94-95 2.8478017975691543 1107 96-97 3.2335679151692567 1107 98-99 3.38134860550813 1107 100-101 3.458617566847945 1108 1 0.23024744602510339 1108 2 0.22411119055929163 1108 3 0.18589721328098818 1108 4 0.14484041787876123 1108 5 0.07580253074335275 1108 6 0.12290760479921659 1108 7 0.1641063301089929 1108 8 0.19515013204723175 1108 9 0.21374025778143135 1108 10-11 0.20102643020286592 1108 12-13 0.25230722326920585 1108 14-15 0.3290171850666681 1108 16-17 0.25944350019008056 1108 18-19 0.28751595402933816 1108 20-21 0.3165483324676046 1108 22-23 0.308371569300121 1108 24-25 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56-57 -0.025323457757131962 2316 58-59 0.12577501506006428 2316 60-61 0.30523015820397603 2316 62-63 0.47706241316480913 2316 64-65 0.5446749571162854 2316 66-67 0.6200846295502345 2316 68-69 0.9327358644149122 2316 70-71 1.0823360029539373 2316 72-73 1.0508271427565 2316 74-75 1.5688267416147674 2316 76-77 1.6872141741931337 2316 78-79 1.625904971711801 2316 80-81 1.5627783662397299 2316 82-83 1.8578946961196827 2316 84-85 1.9724023019734673 2316 86-87 2.164223761513476 2316 88-89 2.4930450207500314 2316 90-91 2.775563141433885 2316 92-93 2.6881244316857433 2316 94-95 3.3372089147325674 2316 96-97 3.7767785609383573 2316 98-99 4.130809159513905 2316 100-101 4.235348345490333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 29.0 5 64.0 6 134.0 7 345.0 8 890.0 9 1652.0 10 2895.0 11 4741.0 12 7382.0 13 10782.0 14 14567.0 15 20327.0 16 27046.0 17 37025.0 18 39139.0 19 33380.0 20 32809.0 21 34824.0 22 38434.0 23 42059.0 24 47608.0 25 53004.0 26 60876.0 27 70958.0 28 84832.0 29 102064.0 30 121859.0 31 146192.0 32 178333.0 33 222310.0 34 291210.0 35 398256.0 36 547681.0 37 641735.0 38 300143.0 39 11083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.720710158370565 18.065648950942514 14.606125044255302 23.607515846431617 2 16.130968024649924 19.278339456010627 39.19341712682119 25.397275392518264 3 19.643999996691093 22.776930176229733 29.413471064831732 28.165598762247445 4 13.519304014830302 16.026039608683433 35.07383854262457 35.38081783386169 5 14.370897635569532 36.51641939531928 33.69463861098889 15.418044358122302 6 34.153485663487636 34.84896947183708 16.17088778619808 14.826657078477199 7 29.935803615644485 32.124414478901386 19.328152108096543 18.611629797357587 8 27.47391826565152 34.358266357402755 18.562507375900136 19.605308001045586 9 26.483035191375926 16.48492121177362 19.41227854425696 37.6197650525935 10-11 25.850653103496196 25.829614601106687 26.189929974599384 22.12980232079773 12-13 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25.23322081932423 28.250694613045418 23.17461840572571 38-39 22.86611898598186 25.641703001618115 28.17233631623706 23.319841696162964 40-41 23.28601025439085 25.50388834610238 28.284315712580337 22.925785686926435 42-43 22.976865323353966 25.543553567403126 28.022691409651085 23.45688969959182 44-45 22.97984028130212 25.765314739422045 28.243997382094648 23.01084759718119 46-47 23.463017456166572 26.026543806959744 27.498632359477693 23.01180637739599 48-49 23.17651606812173 26.10293590508575 27.370291980222156 23.350256046570365 50-51 23.193473707662427 26.281735601998964 27.42967129201221 23.095119398326407 52-53 23.593742934316857 26.00561559816074 27.298987173958363 23.10165429356404 54-55 23.163253263863076 26.19213584640956 27.37386273521487 23.270748154512496 56-57 23.129947788385724 26.46930728860128 27.195066787603604 23.205678135409393 58-59 23.115165258401337 26.043597953392133 27.207457280535056 23.63377950767147 60-61 22.808218196015975 26.318587447927637 27.40471736715964 23.468476988896743 62-63 22.92307518383184 26.278978262236247 27.63094330799154 23.167003245940368 64-65 22.672722520076192 26.405691811031783 27.431587643147292 23.48999802574473 66-67 22.65942460369434 26.37539591963235 27.374270097496805 23.59090937917651 68-69 23.115246174573038 26.006685501291273 27.540468431783125 23.337599892352564 70-71 23.021663867047696 26.163363005985634 27.070844881991373 23.7441282449753 72-73 22.97134875035949 26.2748494974609 26.966335353820558 23.787466398359054 74-75 22.797836811809354 26.738020185932935 26.886516718853915 23.577626283403795 76-77 22.918484213126924 26.306648166755085 27.19234705863538 23.58252056148261 78-79 22.96391138571764 26.41546658049061 27.190995962151653 23.4296260716401 80-81 23.120326822237743 26.468594839193187 26.917076522583592 23.49400181598547 82-83 22.990116283157754 26.518882657040443 27.00084638002615 23.490154679775653 84-85 22.74837410474252 26.658864005517103 26.946355624595945 23.646406265144428 86-87 23.104076254837032 26.58554148795703 26.73566930133695 23.574712955868986 88-89 22.86926569836247 26.726301491941403 26.434643878540285 23.969788931155843 90-91 22.813626616823747 26.600155214528453 26.696967829452074 23.889250339195723 92-93 22.907006209255123 26.3116186333352 26.782200153694163 23.999175003715518 94-95 22.660071288321195 26.102488749355125 26.951529511418293 24.285910450905387 96-97 22.9463511934748 25.785863595868285 26.938805447077062 24.32897976357985 98-99 22.601128193137313 26.210499729229237 26.571077049999392 24.61729502763406 100-101 23.095781159869453 25.373550604353728 26.794835171743664 24.735833064033148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1525.0 1 1030.0 2 746.0 3 1010.5 4 857.5 5 782.5 6 1522.5 7 3445.0 8 5715.5 9 6494.5 10 5282.0 11 3988.0 12 3772.0 13 3874.5 14 4025.0 15 4227.0 16 4394.0 17 4551.0 18 4872.0 19 5282.0 20 5871.0 21 6881.5 22 8233.0 23 9970.0 24 12229.0 25 15037.5 26 18348.0 27 22287.5 28 25714.5 29 28938.5 30 33135.0 31 39935.5 32 49585.0 33 52858.0 34 51698.0 35 54324.5 36 59539.0 37 66026.0 38 73151.0 39 78709.5 40 84383.0 41 92972.0 42 98528.0 43 102571.5 44 109253.5 45 118033.0 46 123780.5 47 129098.0 48 134654.0 49 136203.5 50 137710.0 51 139581.5 52 142577.5 53 147498.5 54 150666.0 55 143513.0 56 133574.5 57 125586.5 58 117087.0 59 104242.5 60 84446.0 61 69078.5 62 57998.5 63 43822.0 64 31172.0 65 23509.0 66 17444.0 67 13235.5 68 10102.5 69 7403.0 70 5462.5 71 3800.0 72 2624.0 73 1883.0 74 1343.0 75 996.0 76 688.5 77 441.5 78 277.0 79 154.0 80 77.5 81 46.0 82 29.5 83 14.5 84 6.5 85 4.0 86 3.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015082648502047599 3 0.0030606471366129502 4 0.0015165368694929032 5 0.11244431552349199 6 0.0 7 0.0 8 2.2058718101714952E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.757339762714369E-5 18-19 0.0 20-21 0.0 22-23 2.0680048220357767E-4 24-25 4.825344584750146E-4 26-27 0.0053492391396658765 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010243517218483883 36-37 6.755482418650205E-4 38-39 0.0066176154305144855 40-41 0.009237088205093138 42-43 0.005556039621869454 44-45 0.003322594414070815 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.3786698813571845E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.757339762714369E-5 68-69 8.272019288143107E-4 70-71 0.0 72-73 2.757339762714369E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.272019288143108E-5 82-83 0.0017233373516964807 84-85 0.00395678255949512 86-87 0.002964140244917947 88-89 0.0 90-91 0.002495392485256504 92-93 2.757339762714369E-5 94-95 8.272019288143108E-5 96-97 1.3786698813571846E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3626684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.98064606526519 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33550618655647 30.494203573469452 2 15.295617386880398 13.454222692842063 3 5.8903446257353735 7.771834865607133 4 2.898698771677738 5.099465789079103 5 1.7006191741534518 3.739716499512327 6 1.077000696562696 2.84203118685408 7 0.6991046181715174 2.152295094207571 8 0.4998241563597472 1.758607145258225 9 0.3708392224663246 1.4678773731368606 >10 2.0094665400754663 15.81859606923903 >50 0.14275934527966389 4.34358634864199 >100 0.07231953638570487 5.710914669438851 >500 0.004012566201794911 1.1849486064518724 >1k 0.0036990844535981977 3.164201291343993 >5k 6.269634667115589E-5 0.2273497598920387 >10k+ 1.2539269334231178E-4 0.7701490350254264 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15354 0.4233619471671643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12576 0.34676304855895906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8245 0.22734266343579973 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4651 0.12824387236384532 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4597 0.12675490889197955 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4295 0.11842774280858216 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4135 0.11401599918823917 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3987 0.10993513633942191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1029359050857477E-4 2 0.0 0.0 0.0 0.0 1.1029359050857477E-4 3 0.0 0.0 0.0 0.0 1.1029359050857477E-4 4 0.0 0.0 0.0 5.514679525428739E-5 1.1029359050857477E-4 5 0.0 0.0 0.0 5.514679525428739E-5 1.1029359050857477E-4 6 0.0 0.0 0.0 5.514679525428739E-5 1.1029359050857477E-4 7 0.0 0.0 0.0 5.514679525428739E-5 1.1029359050857477E-4 8 0.0 0.0 0.0 5.514679525428739E-5 1.1029359050857477E-4 9 0.0 0.0 0.0 5.514679525428739E-5 1.3786698813571846E-4 10-11 0.0 0.0 0.0 5.514679525428739E-5 1.6544038576286215E-4 12-13 0.0 0.0 0.0 8.272019288143108E-5 4.2738766322072724E-4 14-15 0.0 0.0 0.0 8.272019288143108E-5 5.652546513564457E-4 16-17 1.3786698813571847E-5 0.0 0.0 1.240802893221466E-4 6.341881454243049E-4 18-19 4.136009644071554E-5 0.0 0.0 1.6544038576286215E-4 6.617615430514486E-4 20-21 5.514679525428739E-5 0.0 0.0 2.2058718101714957E-4 6.893349406785923E-4 22-23 5.514679525428739E-5 0.0 0.0 3.58454169152868E-4 8.13415230000739E-4 24-25 5.514679525428739E-5 0.0 0.0 5.790413501700175E-4 8.409886276278826E-4 26-27 5.514679525428739E-5 0.0 0.0 0.001075362507458604 9.92642314577173E-4 28-29 6.893349406785923E-5 0.0 0.0 0.0038051288725458295 0.0010615758086450322 30-31 8.272019288143108E-5 0.0 0.0 0.012780269800181102 0.001075362507458604 32-33 8.272019288143108E-5 0.0 0.0 0.030179083702908772 0.0011305093027128915 34-35 8.272019288143108E-5 0.0 0.0 0.055794770098525265 0.001158082700340035 36-37 8.272019288143108E-5 0.0 0.0 0.09929180485534445 0.0011856560979671788 38-39 8.272019288143108E-5 0.0 0.0 0.16357918142302996 0.0012545895920350381 40-41 8.272019288143108E-5 0.0 0.0 0.23193363414071919 0.0012959496884757536 42-43 8.272019288143108E-5 0.0 0.0 0.30110150208840913 0.0014338166766114722 44-45 8.272019288143108E-5 0.0 0.0 0.3853658052369603 0.0014751767730521877 46-47 8.272019288143108E-5 0.0 0.0 0.481748616642641 0.001530323568306475 48-49 8.272019288143108E-5 0.0 0.0 0.5813575155706976 0.0015854703635607625 50-51 8.272019288143108E-5 0.0 0.0 0.6887283259307952 0.001709550652882909 52-53 8.272019288143108E-5 0.0 0.0 0.7922940074183469 0.001806057544577912 54-55 8.272019288143108E-5 0.0 0.0 0.9066408873781118 0.001888777737459343 56-57 8.272019288143108E-5 0.0 0.0 1.0348571863443299 0.0019439245327136305 58-59 1.1029359050857477E-4 0.0 0.0 1.169939261319707 0.001985284629154346 60-61 1.1029359050857477E-4 0.0 0.0 1.3124661536544127 0.0020266447255950617 62-63 1.1029359050857477E-4 0.0 0.0 1.45372466969827 0.0021231516172900643 64-65 1.1029359050857477E-4 0.0 0.0 1.5987469545182322 0.002178298412544352 66-67 1.1029359050857477E-4 0.0 0.0 1.7665035056817744 0.0021920851113579236 68-69 1.1029359050857477E-4 0.0 0.0 1.9499217466975343 0.0022058718101714953 70-71 1.1029359050857477E-4 0.0 0.0 2.1411708326394026 0.00231616540068007 72-73 1.1029359050857477E-4 0.0 0.0 2.333371200799408 0.002398885593561501 74-75 1.1029359050857477E-4 0.0 0.0 2.5275706402873808 0.0024816057864429325 76-77 1.1029359050857477E-4 0.0 0.0 2.7387690794124877 0.0025918993769515073 78-79 1.1029359050857477E-4 0.0 0.0 2.974576775919821 0.002619472774578651 80-81 1.6544038576286215E-4 0.0 1.3786698813571847E-5 3.2174846223161433 0.002702192967460082 82-83 1.6544038576286215E-4 0.0 2.7573397627143693E-5 3.4655211206711143 0.002867633353222944 84-85 1.79227084576434E-4 0.0 2.7573397627143693E-5 3.7215814777355845 0.002895206750850088 86-87 1.9301378339000585E-4 0.0 2.7573397627143693E-5 3.9903393844073536 0.0030330737389858064 88-89 1.9301378339000585E-4 0.0 2.7573397627143693E-5 4.271822414084051 0.003143367329494381 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7150 0.0 53.54769 1 GTATCAA 12495 0.0 42.060745 1 ATCAACG 15875 0.0 32.80042 3 TATCAAC 15905 0.0 32.773876 2 TCAACGC 16005 0.0 32.5322 4 CAACGCA 16355 0.0 31.893713 5 AACGCAG 16990 0.0 30.722042 6 ACGCAGA 19300 0.0 26.87269 7 CGCAGAG 19425 0.0 26.528608 8 TAAGGTA 1125 0.0 23.669577 4 AGGTAAG 1845 0.0 23.200485 2 GTACATG 12240 0.0 23.158785 1 GCAGAGT 21815 0.0 23.143223 9 TGGTATC 3265 0.0 22.724398 2 TACATGG 12165 0.0 22.597828 2 GTGTAAG 1310 0.0 22.509785 1 GAGTACT 13385 0.0 22.301735 12-13 GTGGTAT 3490 0.0 21.804468 1 ACATGGG 11975 0.0 21.562706 3 TAAGGTG 2285 0.0 21.433842 5 >>END_MODULE