FastQCFastQC Report
Thu 26 May 2016
SRR938174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615963
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159150.4401317159495271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125170.3461595154596438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.22375782053079635No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46660.12903893098463673No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45960.12710307046836486No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41420.11454763226283013No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41270.1141328050093433No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36290.10036054019358052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.054.270471
GTATCAA129400.041.347371
TATCAAC160600.033.1139872
ATCAACG160800.032.8963783
TCAACGC161700.032.6838874
CAACGCA166050.031.799055
AACGCAG170450.031.1411766
ACGCAGA193350.027.2974767
CGCAGAG194500.027.0628018
TAAGGTG21550.026.9061475
GTACATG126850.025.9994491
GTAAGGT20700.025.026214
TACATGG126400.024.8907432
TGGTATC34550.023.7971532
GCAGAGT215050.023.658369
ACATGGG126350.023.3966733
GAGTACT132350.023.25822812-13
GTGGTAT37300.023.1604141
AGGTAAG20350.023.1205252
GTACTTT139650.022.06158314-15
Produced by FastQC (version 0.11.5)