##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3615963 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83874143623704 33.0 31.0 34.0 30.0 34.0 2 32.030344060489554 34.0 31.0 34.0 30.0 34.0 3 32.11297875559014 34.0 31.0 34.0 30.0 34.0 4 35.63259358571977 37.0 35.0 37.0 33.0 37.0 5 35.63562763225177 37.0 35.0 37.0 33.0 37.0 6 35.659879263145115 37.0 35.0 37.0 33.0 37.0 7 35.59625333555681 37.0 35.0 37.0 33.0 37.0 8 35.56235226964435 37.0 35.0 37.0 33.0 37.0 9 37.19735323619185 39.0 37.0 39.0 34.0 39.0 10-11 37.10985012844434 39.0 37.0 39.0 33.5 39.0 12-13 37.00519419031666 39.0 37.0 39.0 33.0 39.0 14-15 38.195619258272274 40.0 38.0 41.0 33.0 41.0 16-17 38.049854630702804 40.0 38.0 41.0 33.0 41.0 18-19 38.174509390721084 40.0 38.0 41.0 33.0 41.0 20-21 38.139168321136026 40.0 38.0 41.0 33.0 41.0 22-23 38.05196057039301 40.0 38.0 41.0 33.0 41.0 24-25 37.9396875742368 40.0 38.0 41.0 33.0 41.0 26-27 37.76478976693069 40.0 38.0 41.0 32.5 41.0 28-29 37.55329354863421 40.0 37.5 41.0 32.0 41.0 30-31 37.33865708802883 40.0 37.0 41.0 31.5 41.0 32-33 37.08701167572788 40.0 36.5 41.0 31.0 41.0 34-35 36.85505590073792 39.5 36.0 41.0 30.5 41.0 36-37 36.599003778523176 39.0 35.5 41.0 30.0 41.0 38-39 36.31029728456845 39.0 35.0 40.5 30.0 41.0 40-41 36.097601938957894 39.0 35.0 40.0 29.0 41.0 42-43 35.9321995274841 39.0 35.0 40.0 28.5 41.0 44-45 35.66868950263042 39.0 35.0 40.0 27.5 41.0 46-47 35.28994198779135 38.5 35.0 40.0 26.0 41.0 48-49 34.896601818104884 38.0 34.0 40.0 24.5 41.0 50-51 34.181841047599214 37.5 33.5 39.5 23.5 40.5 52-53 34.303206642324604 38.0 34.0 40.0 24.0 40.5 54-55 34.396749911434384 38.0 34.0 40.0 23.5 41.0 56-57 34.077911057165124 38.0 34.0 40.0 23.0 41.0 58-59 33.73671923634174 37.5 33.0 40.0 22.0 41.0 60-61 33.28827936015938 37.0 33.0 40.0 20.5 41.0 62-63 32.73548636974438 36.0 32.0 39.0 18.0 41.0 64-65 32.19149836433614 35.5 31.5 39.0 13.5 40.0 66-67 31.645255219702193 35.0 31.0 38.5 8.0 40.0 68-69 31.030974183087604 35.0 30.5 38.0 6.5 40.0 70-71 30.48353688906662 35.0 30.0 37.0 2.0 39.5 72-73 29.854213939689096 34.0 29.0 36.5 2.0 39.0 74-75 29.17445477179938 34.0 28.5 36.0 2.0 39.0 76-77 28.524934021725333 34.0 27.5 35.5 2.0 37.5 78-79 27.812329246731785 33.0 26.0 35.0 2.0 37.0 80-81 27.169326400740275 33.0 25.5 35.0 2.0 36.5 82-83 26.460999739211935 32.5 24.5 35.0 2.0 36.0 84-85 25.82339642302756 32.0 22.0 35.0 2.0 35.5 86-87 25.152090604909397 31.5 19.0 34.0 2.0 35.0 88-89 24.34414027466542 31.0 14.5 34.0 2.0 35.0 90-91 23.519729460727334 30.5 4.5 34.0 2.0 35.0 92-93 22.636776427192423 30.0 2.0 34.0 2.0 35.0 94-95 21.684147625404353 29.0 2.0 34.0 2.0 35.0 96-97 20.49012448412774 29.0 2.0 34.0 2.0 35.0 98-99 19.00251385315613 26.0 2.0 33.5 2.0 35.0 100-101 15.398739008114852 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33410954137800886 1101 2 0.3148363745629723 1101 3 0.2838502125974358 1101 4 0.22101555365901504 1101 5 0.24882057111400258 1101 6 0.309773335610096 1101 7 0.3111583644187803 1101 8 0.20721788431820443 1101 9 0.2197639798555855 1101 10-11 0.38801177203765747 1101 12-13 0.34874164701879096 1101 14-15 0.41483556782844033 1101 16-17 0.5544480557570779 1101 18-19 0.5149673895252391 1101 20-21 0.585596061771227 1101 22-23 0.6111802765223828 1101 24-25 0.6253310614819227 1101 26-27 0.6402554140714543 1101 28-29 0.7586879505676905 1101 30-31 0.6730733732984504 1101 32-33 0.655825703534795 1101 34-35 0.7221886505794473 1101 36-37 0.9430701287167693 1101 38-39 0.9127488404635358 1101 40-41 0.9969670288833896 1101 42-43 1.1201801187777036 1101 44-45 0.8850703836372631 1101 46-47 1.0878776120892795 1101 48-49 0.9728839299945662 1101 50-51 0.9274796449044729 1101 52-53 0.9150515587848886 1101 54-55 1.1323127375263837 1101 56-57 1.2243942442521814 1101 58-59 1.3066637334644895 1101 60-61 1.166828459879298 1101 62-63 1.3045564020196636 1101 64-65 1.397815853887984 1101 66-67 1.3488155391123726 1101 68-69 1.6830197024308973 1101 70-71 1.8238214257317367 1101 72-73 1.9926671412348504 1101 74-75 2.175372552217109 1101 76-77 2.111183529166606 1101 78-79 2.195167288668845 1101 80-81 2.3954599223672837 1101 82-83 2.4197110713833005 1101 84-85 2.4634401703060256 1101 86-87 2.713176151625312 1101 88-89 3.0698895296539312 1101 90-91 3.243754652816772 1101 92-93 3.4241569233033964 1101 94-95 3.7744796115862798 1101 96-97 3.893514697744113 1101 98-99 4.02492231854222 1101 100-101 3.964092736439248 1102 1 0.3864394025038145 1102 2 0.36562055825567086 1102 3 0.3277460008304587 1102 4 0.28396505803670635 1102 5 0.32412874439350503 1102 6 0.39705438814982585 1102 7 0.2568279185128546 1102 8 0.3033547688104292 1102 9 0.35784402702489615 1102 10-11 0.43524138125663114 1102 12-13 0.4430894811661261 1102 14-15 0.6243242801817033 1102 16-17 0.6062646222030708 1102 18-19 0.4586669378823487 1102 20-21 0.47563503166350785 1102 22-23 0.4993663176073255 1102 24-25 0.39854443428691155 1102 26-27 0.41784889476050324 1102 28-29 0.4584313687183226 1102 30-31 0.502800545765659 1102 32-33 0.6165626151501797 1102 34-35 0.6056186680966533 1102 36-37 0.6888447296325495 1102 38-39 0.7770725478969567 1102 40-41 0.8678670945552796 1102 42-43 1.056464274359847 1102 44-45 1.0563678861908912 1102 46-47 1.0745440913522515 1102 48-49 1.2169708901434788 1102 50-51 1.3342397091375986 1102 52-53 1.3053358159277408 1102 54-55 1.2887596562369126 1102 56-57 1.387914140982673 1102 58-59 1.224243187088014 1102 60-61 1.3067709067951867 1102 62-63 1.2749629914558582 1102 64-65 1.3246106321360145 1102 66-67 1.2606952001494705 1102 68-69 1.5287905451973884 1102 70-71 1.618930904751494 1102 72-73 1.8054606773792834 1102 74-75 1.7899899595213 1102 76-77 2.0597071608728115 1102 78-79 2.2754869993659064 1102 80-81 2.328874584872377 1102 82-83 2.690771467965785 1102 84-85 2.713061685378218 1102 86-87 2.6012244610727357 1102 88-89 3.048014632297754 1102 90-91 3.2913576084752414 1102 92-93 3.345046369432797 1102 94-95 3.6243650736744115 1102 96-97 3.943983771198095 1102 98-99 4.037917051692464 1102 100-101 4.071949908227374 1103 1 0.5754591359252963 1103 2 0.5158424774694979 1103 3 0.4860031516563339 1103 4 0.2956405222765497 1103 5 0.34764047391719544 1103 6 0.43245299913181867 1103 7 0.5079643721439666 1103 8 0.5564804036646578 1103 9 0.6484146124853112 1103 10-11 0.6650424287015397 1103 12-13 0.6550027036947981 1103 14-15 0.8167046521245069 1103 16-17 0.8709040594338475 1103 18-19 0.7912918169372389 1103 20-21 0.7895276220753615 1103 22-23 0.799684304254292 1103 24-25 0.9112127674997268 1103 26-27 0.8748931512020235 1103 28-29 0.9323809109346257 1103 30-31 0.9501020494448014 1103 32-33 0.9755965693063828 1103 34-35 1.0024341532027634 1103 36-37 1.1402848803103538 1103 38-39 1.1123005444949925 1103 40-41 1.1951807185700787 1103 42-43 1.3322401307889606 1103 44-45 1.2017022456208153 1103 46-47 1.296360867687298 1103 48-49 1.4608106951162583 1103 50-51 1.4224022387067379 1103 52-53 1.4065473330012352 1103 54-55 1.489919351988938 1103 56-57 1.4575947081592915 1103 58-59 1.5833391202333473 1103 60-61 1.62666461282884 1103 62-63 1.730127562888363 1103 64-65 1.8744386404412055 1103 66-67 1.8438827106945084 1103 68-69 2.046034993773766 1103 70-71 2.1421425526695117 1103 72-73 2.222220656857349 1103 74-75 2.3645448298646947 1103 76-77 2.524813334324456 1103 78-79 2.7935893404500582 1103 80-81 2.7891939143974476 1103 82-83 2.907394232848489 1103 84-85 2.9514407970815704 1103 86-87 3.0156010040913195 1103 88-89 3.279478438289747 1103 90-91 3.6252408331731836 1103 92-93 3.749840099913719 1103 94-95 4.027268617150952 1103 96-97 4.1926842783928215 1103 98-99 4.283677516672611 1103 100-101 4.283355075209098 1104 1 0.24082655029453193 1104 2 0.2402515852499647 1104 3 0.33794461190180414 1104 4 0.23185295039158405 1104 5 0.22963860921580448 1104 6 0.18552009975020667 1104 7 0.25320576559960273 1104 8 0.25818388282680615 1104 9 0.3332187537674187 1104 10-11 0.3632589370872381 1104 12-13 0.3548171185341147 1104 14-15 0.5102864769602249 1104 16-17 0.5998395710632423 1104 18-19 0.43519371761039594 1104 20-21 0.5428630793566782 1104 22-23 0.4288888492947436 1104 24-25 0.416096077901706 1104 26-27 0.4597685592681273 1104 28-29 0.43383310830978417 1104 30-31 0.4794989471272544 1104 32-33 0.3096506393997913 1104 34-35 0.38155064289979634 1104 36-37 0.539693194213207 1104 38-39 0.61209882149425 1104 40-41 0.5447107169927321 1104 42-43 0.6310733128951185 1104 44-45 0.7703461620976029 1104 46-47 0.7902925192494692 1104 48-49 0.9134858544514017 1104 50-51 0.9392337456009798 1104 52-53 0.8648277888796017 1104 54-55 1.178017330542275 1104 56-57 1.2795773094854042 1104 58-59 1.2569671825837148 1104 60-61 1.336600160377948 1104 62-63 1.3360136057910736 1104 64-65 1.4646467717261729 1104 66-67 1.5044943556557797 1104 68-69 1.3935103328115588 1104 70-71 1.601870624858762 1104 72-73 1.7762258865586809 1104 74-75 1.984783034795484 1104 76-77 1.9120683449936493 1104 78-79 2.038525156095538 1104 80-81 2.311857480516519 1104 82-83 2.473079510609349 1104 84-85 2.6252074366262583 1104 86-87 2.7236959008377752 1104 88-89 2.805185913389316 1104 90-91 2.882320946096417 1104 92-93 3.0050490563108347 1104 94-95 3.242543811572883 1104 96-97 3.3748421783080254 1104 98-99 3.558586923523695 1104 100-101 3.73724666328188 1105 1 0.25278609147410336 1105 2 0.21072449970583307 1105 3 0.16394671501332425 1105 4 0.11481028527783366 1105 5 0.11077501796594191 1105 6 0.12849265676056376 1105 7 0.16760105524600277 1105 8 0.16835754437990147 1105 9 0.23572286218437455 1105 10-11 0.22019993522916081 1105 12-13 0.19697483617189704 1105 14-15 0.3517582281736509 1105 16-17 0.4196631221572531 1105 18-19 0.3574329504769551 1105 20-21 0.3034595937373794 1105 22-23 0.3290060561200221 1105 24-25 0.344521043789932 1105 26-27 0.3833118063240377 1105 28-29 0.4519856437498362 1105 30-31 0.37990499881219364 1105 32-33 0.4577081416716382 1105 34-35 0.4064682860091793 1105 36-37 0.4077447302340076 1105 38-39 0.4648030271877346 1105 40-41 0.700405879114129 1105 42-43 0.6791158754689448 1105 44-45 0.7449252870549472 1105 46-47 0.6443546167220902 1105 48-49 0.7574594434402471 1105 50-51 0.7715222762616207 1105 52-53 0.7290191540550808 1105 54-55 0.8255474502411886 1105 56-57 0.7672478061814161 1105 58-59 0.8129163516653151 1105 60-61 0.9460980372879959 1105 62-63 0.9337312259212425 1105 64-65 0.9800209405550291 1105 66-67 1.1082032199354472 1105 68-69 1.2852682891635752 1105 70-71 1.4086542747633857 1105 72-73 1.4554982510539354 1105 74-75 1.6430521255551902 1105 76-77 1.8268978246284036 1105 78-79 2.019597272793998 1105 80-81 2.1497534126020312 1105 82-83 2.0997221753379733 1105 84-85 2.2999886647272056 1105 86-87 2.5475473533824093 1105 88-89 2.6880460038394816 1105 90-91 2.7497901387862633 1105 92-93 2.896974903737526 1105 94-95 3.2402104265149205 1105 96-97 3.416180932510514 1105 98-99 3.613135957265431 1105 100-101 3.5262429659072083 1106 1 0.2633423310121934 1106 2 0.21638691396816512 1106 3 0.15375275713348913 1106 4 0.24506395064739905 1106 5 0.28179242329757415 1106 6 0.11935826034164876 1106 7 0.23410446522017736 1106 8 0.1769157315888279 1106 9 0.25485511622736823 1106 10-11 0.16678602355147376 1106 12-13 0.26004439890088094 1106 14-15 0.45490688518918887 1106 16-17 0.18736252666355568 1106 18-19 0.36065139748074415 1106 20-21 0.2813203399228783 1106 22-23 0.2516441951859534 1106 24-25 0.239346043301623 1106 26-27 0.25840129159884384 1106 28-29 0.294802220096706 1106 30-31 0.3391345154816463 1106 32-33 0.33632774158990486 1106 34-35 0.3336229317560111 1106 36-37 0.31469887935887897 1106 38-39 0.11902905745817804 1106 40-41 0.5185680027140265 1106 42-43 0.38801806748224976 1106 44-45 0.4656603080182933 1106 46-47 0.5870364497153631 1106 48-49 0.6682200302962258 1106 50-51 0.6626643889543118 1106 52-53 0.7592854070458017 1106 54-55 0.28540464274900756 1106 56-57 0.566696694703765 1106 58-59 0.7586950960097951 1106 60-61 0.8984876855507977 1106 62-63 0.7280934443313569 1106 64-65 0.9680226277923296 1106 66-67 1.1290092245407806 1106 68-69 0.7980962687790694 1106 70-71 1.0122700069335338 1106 72-73 1.2738172548584323 1106 74-75 1.4038219050951 1106 76-77 1.6047273187889566 1106 78-79 1.9054525845996046 1106 80-81 1.8442849438179358 1106 82-83 1.900423625177698 1106 84-85 1.9778723693695248 1106 86-87 1.8953244910999771 1106 88-89 2.1324604174718154 1106 90-91 2.370717725550165 1106 92-93 2.675931160513837 1106 94-95 2.6418066205887563 1106 96-97 2.710169862844854 1106 98-99 2.922427605936864 1106 100-101 3.094141012996694 1107 1 0.22397824264965394 1107 2 0.12109374587949162 1107 3 0.05685808697070627 1107 4 0.04234819807110313 1107 5 0.1042081324155788 1107 6 0.0515391605928599 1107 7 0.07168850263232684 1107 8 0.15372176688273953 1107 9 0.16486595206335153 1107 10-11 0.13887460920004457 1107 12-13 0.0845116295969035 1107 14-15 0.22053912225069894 1107 16-17 0.2267747669288056 1107 18-19 0.17864140516883964 1107 20-21 0.27355856267484313 1107 22-23 0.2958445840263835 1107 24-25 0.21655372514745608 1107 26-27 0.322657555804021 1107 28-29 0.3590495318058231 1107 30-31 0.3351065922411536 1107 32-33 0.30720611924140684 1107 34-35 0.3720007718893328 1107 36-37 0.3800348587995259 1107 38-39 0.3013822287859611 1107 40-41 0.3219181741082764 1107 42-43 0.36246963315223724 1107 44-45 0.3559715262547982 1107 46-47 0.475587673539998 1107 48-49 0.5973684659230614 1107 50-51 0.2867571105823785 1107 52-53 0.32625475649923175 1107 54-55 0.3337718474643765 1107 56-57 0.37673191227595026 1107 58-59 0.3415413104007783 1107 60-61 0.4730873247688905 1107 62-63 0.5605695485351632 1107 64-65 0.6184996816238808 1107 66-67 0.8107496104073562 1107 68-69 0.8951924253656713 1107 70-71 1.0329194364716372 1107 72-73 1.184319151682356 1107 74-75 1.2566052177013773 1107 76-77 1.3603143763238137 1107 78-79 1.5109350931906853 1107 80-81 1.586935349804424 1107 82-83 1.7102262252183387 1107 84-85 1.8331242130310912 1107 86-87 2.0037235239651423 1107 88-89 2.1775321089129456 1107 90-91 2.521996561982096 1107 92-93 2.7684063554682545 1107 94-95 3.2132113239575624 1107 96-97 3.399548348884192 1107 98-99 3.436670585079977 1107 100-101 3.598407116842406 1108 1 0.34328778990431985 1108 2 0.2411151780955052 1108 3 0.25731986124325346 1108 4 0.21240035729528728 1108 5 0.1945512626029995 1108 6 0.24297997608049826 1108 7 0.2194188452716972 1108 8 0.12400338170205316 1108 9 0.26078820710876727 1108 10-11 0.26777885648036914 1108 12-13 0.30025111773914404 1108 14-15 0.37431619243813685 1108 16-17 0.4502499966655975 1108 18-19 0.4320199956529791 1108 20-21 0.3414757827855368 1108 22-23 0.3681557151790642 1108 24-25 0.3741924798293539 1108 26-27 0.34240147354358186 1108 28-29 0.4570883204357017 1108 30-31 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-2.76776952012073 2316 96-97 -3.3085627657978804 2316 98-99 -3.3671197784856854 2316 100-101 -3.144596696589165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10483.0 3 3235.0 4 3452.0 5 3752.0 6 4380.0 7 5613.0 8 6787.0 9 8460.0 10 10513.0 11 13151.0 12 16084.0 13 19622.0 14 22591.0 15 27423.0 16 32816.0 17 40658.0 18 40000.0 19 34859.0 20 35464.0 21 37628.0 22 40691.0 23 45766.0 24 51323.0 25 57300.0 26 65339.0 27 74848.0 28 87164.0 29 101106.0 30 119815.0 31 144634.0 32 176269.0 33 223583.0 34 294303.0 35 401566.0 36 524043.0 37 555746.0 38 261553.0 39 13942.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41136062123622 17.708955275715248 14.205333018455105 23.674351084593425 2 15.875649428707467 19.012052212698606 39.759492469684105 25.352805888909824 3 19.587958906016148 22.890037428850736 29.118494810679525 28.403508854453598 4 13.249696246237146 15.747214887213342 35.3409167317617 35.66217213478781 5 14.369356989106244 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26-27 22.579778450877246 25.65373206106374 28.522011095026883 23.244478393032132 28-29 22.829068239155145 26.11662770444377 28.359089347784977 22.69521470861611 30-31 22.772981519275962 25.63094966195012 28.317793584903 23.27827523387092 32-33 22.587952923925037 25.62509986200886 28.360796682679172 23.426150531386934 34-35 23.256661707525396 25.70597208068948 28.17524537235201 22.862120839433114 36-37 23.33250654776817 25.254264228400874 28.21370465550363 23.199524568327327 38-39 22.88769753183836 25.6988034777692 28.125900323884927 23.287598666507517 40-41 23.298921077439886 25.51908833201294 28.261746325400654 22.92024426514652 42-43 23.001324718742765 25.556684767560128 28.001384191394614 23.440606322302493 44-45 22.996909609750066 25.797719257144642 28.188519060541278 23.016852072564014 46-47 23.48976477538052 26.067544003907063 27.46938685747947 22.973304363232945 48-49 23.19249945781346 26.20091552069825 27.32454179704962 23.28204322443867 50-51 23.199893233167973 26.360232950844388 27.3588624077027 23.08101140828494 52-53 23.621103333078405 26.03932669346236 27.27341499092143 23.066154982537803 54-55 23.15730835262823 26.289471333141744 27.353675702423285 23.199544611806736 56-57 23.11839927820261 26.517203123621357 27.18092978988051 23.183467808295525 58-59 23.138352528179624 26.080254639303856 27.210260804482516 23.571132028034 60-61 22.802492028474003 26.269234556482218 27.48976037847323 23.43851303657055 62-63 22.96496551815701 26.22890920763261 27.728308579372406 23.07781669483797 64-65 22.70133398389527 26.378516408986606 27.53710858409841 23.383041023019715 66-67 22.715022834283285 26.359671491662834 27.440178934931144 23.485126739122737 68-69 23.133581577869414 25.92116046345741 27.69911998851618 23.246137970157 70-71 23.07634221644189 26.13339709890616 27.18528011028604 23.604980574365907 72-73 23.006889409869547 26.301901530230094 27.113083351127564 23.578125708772795 74-75 22.909839857314125 26.73162689087205 27.044447170366322 23.314086081447503 76-77 23.191304643643115 26.324409211006323 27.04854284079321 23.435743304557352 78-79 23.23127958585303 26.49378089447968 27.076726526280474 23.198212993386818 80-81 23.458823806010347 26.49259856763423 26.796303813118932 23.25227381323649 82-83 23.345867625155066 26.521699445291897 26.860166813491677 23.272266116061367 84-85 23.155878691793333 26.692778543085083 26.784108338933116 23.367234426188475 86-87 23.56284487298054 26.59313617034457 26.547600215054718 23.29641874162017 88-89 23.400290127049534 26.616957605157225 26.422460724410286 23.56029154338296 90-91 23.35118749246164 26.5896121658362 26.510314138037494 23.54888620366467 92-93 23.576775566178068 26.25201154400835 26.595626686198027 23.57558620361555 94-95 23.359584554234573 26.03984545824739 26.80318998256066 23.797380004957375 96-97 23.747058101051945 25.68884971889443 26.719842147135335 23.844250032918286 98-99 23.45300732173448 26.066152032833145 26.33062406936188 24.150216576070495 100-101 24.398326539214743 24.96380916719957 26.45360857291383 24.184255720671853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1621.0 1 1092.5 2 791.0 3 1015.0 4 834.5 5 798.5 6 1523.5 7 3405.5 8 5743.5 9 6583.5 10 5357.5 11 4090.0 12 3790.0 13 3874.0 14 4133.5 15 4268.5 16 4378.5 17 4562.5 18 4881.5 19 5248.0 20 5837.0 21 6958.0 22 8342.5 23 10212.0 24 12546.5 25 15404.0 26 18897.5 27 22639.0 28 25699.0 29 28793.5 30 32774.0 31 39326.5 32 49275.5 33 52881.5 34 51827.5 35 54542.0 36 59757.0 37 66386.0 38 73119.0 39 78707.0 40 84521.5 41 92341.5 42 97364.5 43 101395.5 44 107804.0 45 116690.5 46 122741.5 47 127903.5 48 133572.5 49 135031.5 50 136511.0 51 138273.0 52 140833.0 53 145310.0 54 148242.5 55 141947.5 56 132623.0 57 124497.5 58 116138.0 59 103898.5 60 84857.0 61 69617.5 62 58469.0 63 44736.0 64 32535.0 65 25056.0 66 18858.5 67 14092.5 68 10810.0 69 8052.5 70 5925.0 71 4163.5 72 2915.0 73 2133.5 74 1556.0 75 1155.5 76 821.0 77 539.5 78 347.5 79 210.5 80 111.0 81 68.5 82 46.5 83 35.0 84 23.5 85 14.0 86 11.0 87 11.5 88 10.5 89 8.5 90 6.5 91 3.5 92 3.5 93 3.0 94 1.0 95 1.5 96 1.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4615091470792152 2 0.039823416334735726 3 0.05271071634305993 4 0.055752782868630016 5 0.08230172709178717 6 0.08136145198388368 7 0.04474603307611278 8 0.03987872663520064 9 0.04593520453610836 10-11 0.04825823715563461 12-13 0.04416527492123122 14-15 0.03856510699915901 16-17 0.03823324519636954 18-19 0.04619792846331669 20-21 0.05466040443444802 22-23 0.04636385936471142 24-25 0.06464391366836442 26-27 0.055351783190259414 28-29 0.04326648253867642 30-31 0.045064067303786016 32-33 0.04426206794704481 34-35 0.04872837470958635 36-37 0.043238827388443965 38-39 0.026120289394554096 40-41 0.024198256453398445 42-43 0.02367280859898179 44-45 0.015542194430639915 46-47 0.02686697845083039 48-49 0.026853150875714157 50-51 0.016814331341332863 52-53 0.016081469860172795 54-55 0.016205918036218844 56-57 0.015044401726455721 58-59 0.013716954515297862 60-61 0.016454814388310944 62-63 0.01609529743528902 64-65 0.017339779195749513 66-67 0.009596337130662012 68-69 0.012223576402745271 70-71 0.017616330698074067 72-73 0.015459228979942551 74-75 0.012956437883905339 76-77 0.009181509877175182 78-79 0.014698712348550027 80-81 0.017021744968076277 82-83 0.01515502232738554 84-85 0.013675471789949177 86-87 0.003844065882311296 88-89 0.02751687448129309 90-91 0.03094611311011755 92-93 0.01606764228505657 94-95 0.03172045731662631 96-97 0.026300047871065053 98-99 0.023119705594332688 100-101 0.14496829751853102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3615963.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.98691393179484 #Duplication Level Percentage of deduplicated Percentage of total 1 71.10792463989385 32.70034010283352 2 14.583331648709287 13.41284834736027 3 5.566619275802434 7.679749245821899 4 2.7409499348575483 5.041913149826109 5 1.5220630420402745 3.499749105653597 6 0.9838619017198794 2.714686355709845 7 0.6497505809117121 2.091601682906442 8 0.46017947810353804 1.6929787242180538 9 0.3480905788891527 1.4406850342659694 >10 1.8360465957641583 15.143778071988223 >50 0.12741777512211108 4.013515040577094 >100 0.06657961723355971 5.417213934812964 >500 0.0036830318412489353 1.1500586762463856 >1k 0.003320766398763222 2.986792887946683 >5k 6.037757088660404E-5 0.22465293858360164 >10k+ 1.2075514177320807E-4 0.7894367012494083 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15915 0.4401317159495271 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12517 0.3461595154596438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8091 0.22375782053079635 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4666 0.12903893098463673 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4596 0.12710307046836486 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4142 0.11454763226283013 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4127 0.1141328050093433 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3629 0.10036054019358052 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7655150232455364E-5 2 0.0 0.0 0.0 0.0 2.7655150232455364E-5 3 0.0 0.0 0.0 0.0 2.7655150232455364E-5 4 0.0 0.0 0.0 0.0 2.7655150232455364E-5 5 0.0 0.0 0.0 0.0 5.531030046491073E-5 6 0.0 0.0 0.0 0.0 8.29654506973661E-5 7 0.0 0.0 0.0 0.0 8.29654506973661E-5 8 0.0 0.0 0.0 0.0 8.29654506973661E-5 9 0.0 0.0 0.0 0.0 8.29654506973661E-5 10-11 0.0 0.0 0.0 5.531030046491073E-5 8.29654506973661E-5 12-13 0.0 0.0 0.0 5.531030046491073E-5 4.8396512906796887E-4 14-15 0.0 0.0 0.0 1.1062060092982146E-4 6.91378755811384E-4 16-17 0.0 0.0 0.0 1.2444817604604914E-4 8.573096572061162E-4 18-19 0.0 0.0 0.0 1.521033262785045E-4 9.402751079034824E-4 20-21 0.0 0.0 0.0 2.0741362674341524E-4 0.0011062060092982147 22-23 0.0 0.0 0.0 3.04206652557009E-4 0.001175343884879353 24-25 0.0 0.0 0.0 6.222408802302457E-4 0.0012721369106929467 26-27 0.0 0.0 0.0 0.0011200335844144423 0.0013551023613903128 28-29 1.3827575116227682E-5 0.0 0.0 0.0038855486076599787 0.0013827575116227683 30-31 2.7655150232455364E-5 0.0 0.0 0.012458645179721142 0.0013965850867389959 32-33 4.148272534868305E-5 0.0 0.0 0.030641906457560545 0.0014657229623201344 34-35 9.679302581359377E-5 0.0 0.0 0.05930646967350053 0.0015486884130175005 36-37 1.1062060092982146E-4 0.0 0.0 0.10255912463706071 0.001562515988133728 38-39 1.1062060092982146E-4 0.0 0.0 0.16804652038751502 0.001603998713482411 40-41 1.1062060092982146E-4 0.0 0.0 0.23616115541005261 0.001603998713482411 42-43 1.1062060092982146E-4 0.0 0.0 0.30312810169794324 0.0016178262885986389 44-45 1.1062060092982146E-4 0.0 0.0 0.3879741026111163 0.0016869641641797774 46-47 1.1062060092982146E-4 0.0 0.0 0.4872975746709798 0.0017561020397609158 48-49 1.1062060092982146E-4 0.0 0.0 0.58934507902874 0.0018805502158069647 50-51 1.1062060092982146E-4 0.0 0.0 0.6978777161160112 0.0019358605162718755 52-53 1.1062060092982146E-4 0.0 0.0 0.8009761161826048 0.002046481117201697 54-55 1.3827575116227682E-4 0.0 0.0 0.9142377839596256 0.00208796384255038 56-57 1.3827575116227682E-4 0.0 0.0 1.042281129535894 0.0021156189927828356 58-59 1.3827575116227682E-4 0.0 0.0 1.1768234354167895 0.002129446567899063 60-61 1.3827575116227682E-4 0.0 0.0 1.3175466673746383 0.0021432741430152907 62-63 1.3827575116227682E-4 0.0 0.0 1.4575232102762112 0.0023783429199911612 64-65 1.3827575116227682E-4 0.0 0.0 1.603611541379157 0.0024613083706885273 66-67 1.3827575116227682E-4 0.0 0.0 1.7716718893417882 0.0024613083706885273 68-69 1.3827575116227682E-4 0.0 0.0 1.9563391550190086 0.0024613083706885273 70-71 1.3827575116227682E-4 0.0 0.0 2.1433847636162207 0.002488963520920983 72-73 1.3827575116227682E-4 0.0 0.0 2.3366251258655026 0.002654894422315715 74-75 1.3827575116227682E-4 0.0 0.0 2.5305430392954795 0.0028208253237104472 76-77 1.3827575116227682E-4 0.0 0.0 2.737002563355875 0.0028899631992915856 78-79 1.3827575116227682E-4 0.0 0.0 2.9627238995531755 0.0029314459246402684 80-81 1.659309013947322E-4 2.7655150232455364E-5 0.0 3.2041533610825113 0.0029867562251051794 82-83 1.659309013947322E-4 2.7655150232455364E-5 0.0 3.4548887806650677 0.0032356525771972777 84-85 1.659309013947322E-4 2.7655150232455364E-5 0.0 3.7114732645217883 0.0032494801523135055 86-87 1.659309013947322E-4 2.7655150232455364E-5 0.0 3.970712642800825 0.0033601007532433266 88-89 1.797584765109599E-4 2.7655150232455364E-5 0.0 4.246310042442359 0.0034568937790569205 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7405 0.0 54.27047 1 GTATCAA 12940 0.0 41.34737 1 TATCAAC 16060 0.0 33.113987 2 ATCAACG 16080 0.0 32.896378 3 TCAACGC 16170 0.0 32.683887 4 CAACGCA 16605 0.0 31.79905 5 AACGCAG 17045 0.0 31.141176 6 ACGCAGA 19335 0.0 27.297476 7 CGCAGAG 19450 0.0 27.062801 8 TAAGGTG 2155 0.0 26.906147 5 GTACATG 12685 0.0 25.999449 1 GTAAGGT 2070 0.0 25.02621 4 TACATGG 12640 0.0 24.890743 2 TGGTATC 3455 0.0 23.797153 2 GCAGAGT 21505 0.0 23.65836 9 ACATGGG 12635 0.0 23.396673 3 GAGTACT 13235 0.0 23.258228 12-13 GTGGTAT 3730 0.0 23.160414 1 AGGTAAG 2035 0.0 23.120525 2 GTACTTT 13965 0.0 22.061583 14-15 >>END_MODULE