FastQCFastQC Report
Thu 26 May 2016
SRR938174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615963
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156090.4316692399783958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129440.35796826460890224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.23293933040797155No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46720.12920486188603147No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45930.1270201050176675No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42140.1165388030795669No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41180.11388390865725119No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38380.1061404665921637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71350.054.397021
GTATCAA123100.040.8295561
TATCAAC153750.032.504812
ATCAACG154900.032.106153
TCAACGC158150.031.5930444
CAACGCA159700.031.2864115
AACGCAG166350.030.0642596
ACGCAGA189850.026.1927457
CGCAGAG190550.025.9968248
TAAGGTG23650.024.701255
GTAAGGT22450.023.6944484
TGGTATC30400.023.4403842
GTACATG124800.022.572891
GCAGAGT217100.022.2268969
AGGTAAG21000.022.1693942
TACATGG125050.021.9959092
GAGTACT132650.021.96606312-13
GTGGTAT34850.021.6741141
CAGAGTA200250.020.99015610-11
ACATGGG124350.020.971023
Produced by FastQC (version 0.11.5)