##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938174_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3615963 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50522530236067 34.0 31.0 34.0 31.0 34.0 2 32.73430646275971 34.0 31.0 34.0 31.0 34.0 3 32.698421416369584 34.0 31.0 34.0 31.0 34.0 4 36.22462951086612 37.0 35.0 37.0 35.0 37.0 5 36.18159394883189 37.0 35.0 37.0 35.0 37.0 6 36.24245546760296 37.0 37.0 37.0 35.0 37.0 7 36.20310274192518 37.0 37.0 37.0 35.0 37.0 8 36.19361398332892 37.0 37.0 37.0 35.0 37.0 9 37.90327611206199 39.0 38.0 39.0 35.0 39.0 10-11 37.87080730638007 39.0 38.0 39.0 35.0 39.0 12-13 37.81004313373782 39.0 38.0 39.0 35.0 39.0 14-15 39.10263476147294 40.0 38.5 41.0 36.0 41.0 16-17 39.03618800856093 40.0 38.0 41.0 36.0 41.0 18-19 39.023202532769275 40.0 38.5 41.0 36.0 41.0 20-21 39.020536852838376 40.0 38.5 41.0 35.5 41.0 22-23 39.04488513848178 40.0 39.0 41.0 35.0 41.0 24-25 38.98983756194408 40.0 38.5 41.0 35.0 41.0 26-27 38.82064404420068 40.0 38.0 41.0 35.0 41.0 28-29 38.7416866544265 40.0 38.0 41.0 35.0 41.0 30-31 38.527422432143254 40.0 38.0 41.0 34.0 41.0 32-33 38.33364307101594 40.0 38.0 41.0 34.0 41.0 34-35 38.1306820617357 40.0 38.0 41.0 34.0 41.0 36-37 38.06298322189691 40.0 38.0 41.0 33.5 41.0 38-39 38.023188566918414 40.0 38.0 41.0 33.0 41.0 40-41 37.897744252360994 40.0 37.5 41.0 33.0 41.0 42-43 37.71384497020573 40.0 37.0 41.0 33.0 41.0 44-45 37.467939799162764 40.0 37.0 41.0 33.0 41.0 46-47 37.121686394468085 40.0 36.5 41.0 31.5 41.0 48-49 36.751910625191684 39.5 36.0 41.0 31.0 41.0 50-51 36.447882624905176 39.0 35.5 41.0 30.0 41.0 52-53 35.96866948030166 39.0 35.0 40.0 29.0 41.0 54-55 35.638270081856476 38.5 35.0 40.0 28.0 41.0 56-57 35.34273387200035 38.0 35.0 40.0 28.0 41.0 58-59 34.937373114713836 38.0 34.0 40.0 27.5 41.0 60-61 34.49783446346105 37.0 34.0 40.0 26.0 41.0 62-63 34.041806428882154 36.5 33.5 39.5 26.0 41.0 64-65 33.579217624737865 36.0 33.0 39.0 25.0 40.5 66-67 33.04480134337658 35.5 32.5 39.0 23.5 40.0 68-69 32.50184307748724 35.0 32.0 38.0 22.0 40.0 70-71 31.89544126972538 35.0 31.0 37.0 20.5 39.5 72-73 31.422163473464746 35.0 31.0 37.0 19.5 39.0 74-75 30.871961770626527 34.0 30.0 36.0 17.5 39.0 76-77 29.687452830684386 33.0 28.5 35.0 17.5 37.0 78-79 30.392306834998035 34.0 30.0 35.0 17.5 37.0 80-81 30.21740073114686 34.0 30.0 35.0 13.0 37.0 82-83 29.775092554874043 34.0 30.0 35.0 7.5 36.5 84-85 29.283668140409624 34.0 29.0 35.0 4.5 36.0 86-87 28.759425220888602 34.0 29.0 35.0 2.0 35.5 88-89 28.13453525381759 33.0 27.5 35.0 2.0 35.0 90-91 27.60576905792454 33.0 26.5 35.0 2.0 35.0 92-93 26.9748441839698 33.0 25.5 35.0 2.0 35.0 94-95 26.22444906101086 32.0 24.5 34.0 2.0 35.0 96-97 25.39270092088885 32.0 21.5 34.0 2.0 35.0 98-99 24.34325116158545 31.5 10.0 34.0 2.0 35.0 100-101 20.811816796797977 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05016004124451712 1101 2 0.06621576453267153 1101 3 0.15887269923570102 1101 4 0.004489636161970623 1101 5 0.01934670227969093 1101 6 0.07209427880965791 1101 7 0.048435159484753854 1101 8 0.1071272661259357 1101 9 0.0406680829940953 1101 10-11 0.12689833695933572 1101 12-13 0.10214994548466905 1101 14-15 0.22577958978926205 1101 16-17 0.20371998555251736 1101 18-19 0.2752005477723998 1101 20-21 0.20117062785674023 1101 22-23 0.08111268728864474 1101 24-25 0.186080321593046 1101 26-27 0.19433543455860303 1101 28-29 0.13555144616490367 1101 30-31 0.17751752319767888 1101 32-33 0.19111510227671147 1101 34-35 0.21917135735333915 1101 36-37 0.2321878300493978 1101 38-39 0.32884438091122803 1101 40-41 0.29606390019349504 1101 42-43 0.3447625952507565 1101 44-45 0.4924871019412862 1101 46-47 0.444436255354546 1101 48-49 0.47472144550212647 1101 50-51 0.5665755527063467 1101 52-53 0.5209033685990221 1101 54-55 0.4866716143109784 1101 56-57 0.6090240291060312 1101 58-59 0.5455430388028404 1101 60-61 0.6724020601373013 1101 62-63 0.6385143306049841 1101 64-65 0.6617946053316643 1101 66-67 0.6721905104714949 1101 68-69 0.7708875513563598 1101 70-71 0.8495363924829036 1101 72-73 0.7828019961137791 1101 74-75 0.8716757814402989 1101 76-77 0.9796314660473548 1101 78-79 0.9875544629277861 1101 80-81 1.1381274311502416 1101 82-83 1.2607534786068015 1101 84-85 1.3995230457105556 1101 86-87 1.5622978741101115 1101 88-89 1.7985094236143908 1101 90-91 1.8042875809527104 1101 92-93 2.1382490563189336 1101 94-95 2.08439573212776 1101 96-97 2.224157827536807 1101 98-99 2.324058008142188 1101 100-101 2.5762413742318024 1102 1 0.08229312446444936 1102 2 0.17004446224427028 1102 3 0.2608848909541095 1102 4 0.12184998867436292 1102 5 0.11545094225797214 1102 6 0.06221840655126698 1102 7 0.06527025773768713 1102 8 0.089182959681807 1102 9 0.10430040178832911 1102 10-11 0.07895367182857882 1102 12-13 0.1336436395519769 1102 14-15 0.2240374911218197 1102 16-17 0.2163480220716565 1102 18-19 0.22193764800052662 1102 20-21 0.24077638288303405 1102 22-23 0.24156571253837456 1102 24-25 0.18698550157213134 1102 26-27 0.17746109236066587 1102 28-29 0.0754904271200516 1102 30-31 0.10279317286884293 1102 32-33 0.15280611026474133 1102 34-35 0.19078790965337333 1102 36-37 0.23252062273315488 1102 38-39 0.29649538042780677 1102 40-41 0.2074317778708732 1102 42-43 0.26664618773518356 1102 44-45 0.29919314151892706 1102 46-47 0.320295062995541 1102 48-49 0.2897570753770182 1102 50-51 0.39173309775024023 1102 52-53 0.43644269123342383 1102 54-55 0.36349751567738764 1102 56-57 0.41030775446571255 1102 58-59 0.49819186907131296 1102 60-61 0.5786424821462361 1102 62-63 0.47183859601778266 1102 64-65 0.5273869148484067 1102 66-67 0.6005577165182245 1102 68-69 0.7009357987389038 1102 70-71 0.8587994748222343 1102 72-73 0.8990560119431343 1102 74-75 0.931649295051951 1102 76-77 0.9974149176565952 1102 78-79 1.130388518571369 1102 80-81 1.2449745877223997 1102 82-83 1.2217505891643015 1102 84-85 1.3198500792572254 1102 86-87 1.3191286882743647 1102 88-89 1.5192950521290882 1102 90-91 1.5840934327437992 1102 92-93 1.6534937420641036 1102 94-95 1.7224346210132886 1102 96-97 1.8950101959466714 1102 98-99 2.034141626460112 1102 100-101 2.681140388830954 1103 1 0.24962262198700813 1103 2 0.3039170127829678 1103 3 0.39601940257689705 1103 4 0.1977332753512897 1103 5 0.1461202120421845 1103 6 0.17946302729150432 1103 7 0.21824473272399558 1103 8 0.17581615769434933 1103 9 0.2250020728107458 1103 10-11 0.3066787674656055 1103 12-13 0.26879261293888135 1103 14-15 0.3783877275027976 1103 16-17 0.2884187400982441 1103 18-19 0.3584200304728711 1103 20-21 0.3661381852320318 1103 22-23 0.42107580306213066 1103 24-25 0.47214394397126824 1103 26-27 0.4552593576376296 1103 28-29 0.4116942484690611 1103 30-31 0.43046007712163714 1103 32-33 0.483127092562853 1103 34-35 0.5148076947240057 1103 36-37 0.49321561379785095 1103 38-39 0.625078368572602 1103 40-41 0.6557593781477138 1103 42-43 0.6032685394246258 1103 44-45 0.5794168512368927 1103 46-47 0.6396168406906781 1103 48-49 0.7551065163609607 1103 50-51 0.7128095694929826 1103 52-53 0.7780352413589284 1103 54-55 0.8745463034716607 1103 56-57 0.9218955873014991 1103 58-59 1.0296981995363907 1103 60-61 1.0480055791674516 1103 62-63 0.9966470637250069 1103 64-65 0.8312363265645075 1103 66-67 1.0056980128466577 1103 68-69 1.0965958921894483 1103 70-71 1.2484303899420155 1103 72-73 1.1773315154363395 1103 74-75 1.2226158573873143 1103 76-77 0.8886392092883995 1103 78-79 1.2571958086778032 1103 80-81 1.4356799774841598 1103 82-83 1.3923913141807454 1103 84-85 1.3909420457472486 1103 86-87 1.5188366983700519 1103 88-89 1.6788682234906673 1103 90-91 1.6821650253627887 1103 92-93 2.0088323711577267 1103 94-95 1.9905676072642855 1103 96-97 2.177577744436622 1103 98-99 2.4500304568132876 1103 100-101 2.6700351312910406 1104 1 0.09966616812639728 1104 2 0.12100011380949383 1104 3 0.14652662530146898 1104 4 0.10851725682712754 1104 5 0.019382986574171923 1104 6 0.04995592377871105 1104 7 0.062309469409107976 1104 8 0.05500693351320507 1104 9 0.08141808758675495 1104 10-11 0.10379077084309785 1104 12-13 0.02982524729550562 1104 14-15 0.14710395191455916 1104 16-17 0.09247407519008277 1104 18-19 0.12054847622708564 1104 20-21 0.14134577772138357 1104 22-23 0.11264680096889634 1104 24-25 0.08085129777674638 1104 26-27 0.14509335132097334 1104 28-29 0.14199871745343984 1104 30-31 0.10279627934647095 1104 32-33 0.20897127078499267 1104 34-35 0.2004022778237342 1104 36-37 0.2971391391766858 1104 38-39 0.20283652065456437 1104 40-41 0.3197367518547978 1104 42-43 0.3770135750866501 1104 44-45 0.39099554324933905 1104 46-47 0.4930764031678052 1104 48-49 0.39381619713886806 1104 50-51 0.4975869531951176 1104 52-53 0.44416967164892185 1104 54-55 0.5144174922734521 1104 56-57 0.6056560788496981 1104 58-59 0.5391895400234787 1104 60-61 0.7105889037811508 1104 62-63 0.7893190787842101 1104 64-65 0.736323962844665 1104 66-67 0.6986701396734176 1104 68-69 0.9073612370628936 1104 70-71 1.0102460218868146 1104 72-73 1.180446743258397 1104 74-75 1.123023624165338 1104 76-77 1.014616654834903 1104 78-79 1.2875013708706966 1104 80-81 1.2619131297092814 1104 82-83 1.1607139314677042 1104 84-85 1.3789086310479668 1104 86-87 1.536541782986994 1104 88-89 1.521298144920209 1104 90-91 1.4770701010258307 1104 92-93 1.686450228146274 1104 94-95 1.941478796565832 1104 96-97 2.1935483235924664 1104 98-99 2.544912054521923 1104 100-101 2.6456332595098218 1105 1 0.10490950467607263 1105 2 0.08703388394579292 1105 3 0.12197144237361357 1105 4 0.026269565620253843 1105 5 0.017878265324704046 1105 6 0.06338223520257458 1105 7 0.03571849186342746 1105 8 0.04917796401056762 1105 9 0.08059608325697099 1105 10-11 -0.00329818005472049 1105 12-13 -0.02238546695524235 1105 14-15 0.08397365833103976 1105 16-17 0.10558245668160282 1105 18-19 0.15280305959323925 1105 20-21 0.144985182835768 1105 22-23 0.08592941367977147 1105 24-25 0.0932671335999018 1105 26-27 0.03359478246701286 1105 28-29 0.07474620654914332 1105 30-31 0.1608333554726613 1105 32-33 0.1840975160473377 1105 34-35 0.08359582361743634 1105 36-37 0.2461023385763994 1105 38-39 0.18184985549844868 1105 40-41 0.26841098604283076 1105 42-43 0.28282904911556983 1105 44-45 0.31291407808699745 1105 46-47 0.27883479503367425 1105 48-49 0.3060589908206808 1105 50-51 0.3534438604144654 1105 52-53 0.2856123377896864 1105 54-55 0.42750543799115803 1105 56-57 0.4158497830266654 1105 58-59 0.47564872568316474 1105 60-61 0.47857471154944164 1105 62-63 0.49521078378567296 1105 64-65 0.5945669291915081 1105 66-67 0.7325726631986313 1105 68-69 0.5929114841135075 1105 70-71 0.6731649131269393 1105 72-73 0.6545317028016662 1105 74-75 0.5586065668493667 1105 76-77 0.5934536900093086 1105 78-79 0.9367913982291185 1105 80-81 1.0430983452172349 1105 82-83 0.9986463703340895 1105 84-85 1.2110849936214407 1105 86-87 1.3283941193969007 1105 88-89 1.396885362508165 1105 90-91 1.4597413751959287 1105 92-93 1.7357479821046908 1105 94-95 1.9227360949132084 1105 96-97 1.9798363044850582 1105 98-99 2.135336341945667 1105 100-101 2.429673706455521 1106 1 0.016901415114219276 1106 2 0.020956778875429904 1106 3 0.0881783301586907 1106 4 0.027654048927864494 1106 5 -0.004659932369776243 1106 6 0.016980264092715913 1106 7 0.0453049265003429 1106 8 -0.016977426142133822 1106 9 0.061854479525827344 1106 10-11 0.09966933613169005 1106 12-13 0.027380437395578383 1106 14-15 0.02744927105519679 1106 16-17 0.09961790850744734 1106 18-19 0.018756830779437905 1106 20-21 0.03574121830557431 1106 22-23 0.0471362875130481 1106 24-25 0.08064401286818423 1106 26-27 0.054582005411454304 1106 28-29 0.030357014665121085 1106 30-31 0.026718220284401184 1106 32-33 0.09743577488168853 1106 34-35 -0.0578889607079347 1106 36-37 0.012377807194006607 1106 38-39 0.10903232732899681 1106 40-41 0.01951170530445978 1106 42-43 -0.024308984691806756 1106 44-45 0.14525952085656968 1106 46-47 -0.14284919614755154 1106 48-49 0.13259196000178264 1106 50-51 0.187281602877313 1106 52-53 0.05786599633836431 1106 54-55 0.19027652527949357 1106 56-57 0.3685838081417572 1106 58-59 0.33816764199439575 1106 60-61 0.3073281867095048 1106 62-63 0.30599411207052185 1106 64-65 0.32100513466799185 1106 66-67 0.2927866806619406 1106 68-69 0.05622833469069377 1106 70-71 0.18016662237707237 1106 72-73 0.21310188855588663 1106 74-75 0.45824888928407503 1106 76-77 0.5138938009912017 1106 78-79 0.7153577797934005 1106 80-81 0.8515617222155569 1106 82-83 0.9210322144069458 1106 84-85 0.8787863668813038 1106 86-87 0.9628676607442905 1106 88-89 1.017222633245261 1106 90-91 0.9931856149795699 1106 92-93 1.024734030617882 1106 94-95 1.5508274643006708 1106 96-97 1.5898443624228378 1106 98-99 1.9587618584179367 1106 100-101 2.452229653935998 1107 1 0.05862100466401188 1107 2 0.0912537674325904 1107 3 0.17577559368643136 1107 4 0.0891763387273059 1107 5 0.08167521342033268 1107 6 -0.0019481125493285845 1107 7 0.05677347921846376 1107 8 0.06835445062377943 1107 9 0.0708185438382003 1107 10-11 0.06807199862611668 1107 12-13 0.10470126700239035 1107 14-15 0.06814857078580161 1107 16-17 0.14769763862689445 1107 18-19 0.09846911892395838 1107 20-21 0.09413960275950473 1107 22-23 0.12133947483743412 1107 24-25 0.07372539127847233 1107 26-27 0.02495635385026418 1107 28-29 0.062052203321108834 1107 30-31 0.08346466658556295 1107 32-33 0.11766911598122931 1107 34-35 0.14414555481172187 1107 36-37 0.19516471406166858 1107 38-39 0.18535114052422585 1107 40-41 0.16379422132279586 1107 42-43 0.09747417715885831 1107 44-45 0.10105441629804801 1107 46-47 0.16950158637428814 1107 48-49 0.13449928376250142 1107 50-51 0.10905793430504929 1107 52-53 0.03816553138329937 1107 54-55 0.1310461481561518 1107 56-57 0.16600985868217322 1107 58-59 0.28705585250758503 1107 60-61 0.33676277800485366 1107 62-63 0.3994189715973917 1107 64-65 0.33592422989263326 1107 66-67 0.4202343778220623 1107 68-69 0.49810106718554437 1107 70-71 0.5517528293142746 1107 72-73 0.5466837549693189 1107 74-75 0.5933491653451597 1107 76-77 0.6326486244708214 1107 78-79 0.7674736119769072 1107 80-81 0.827668468094501 1107 82-83 1.0761887710898037 1107 84-85 1.1032727846553172 1107 86-87 1.210852022833631 1107 88-89 1.2789994760336825 1107 90-91 1.2806535794118545 1107 92-93 1.4819266206921462 1107 94-95 1.761930489920136 1107 96-97 1.600012887450852 1107 98-99 1.722807366865922 1107 100-101 1.9161142532406927 1108 1 0.06654785700155941 1108 2 0.08853690684322402 1108 3 0.17701305875426954 1108 4 0.11591261919485163 1108 5 0.008088681359438965 1108 6 0.04903152173280745 1108 7 0.08862138410686526 1108 8 0.047168459685430264 1108 9 0.053953353090456346 1108 10-11 0.055674852301144995 1108 12-13 0.07434502798818698 1108 14-15 0.19806195232117574 1108 16-17 0.15567582193983753 1108 18-19 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Count 2 5.0 3 9.0 4 20.0 5 64.0 6 122.0 7 296.0 8 743.0 9 1460.0 10 2520.0 11 4006.0 12 6161.0 13 8977.0 14 12409.0 15 16806.0 16 22728.0 17 31212.0 18 31714.0 19 25315.0 20 23063.0 21 23062.0 22 24875.0 23 27189.0 24 30538.0 25 35994.0 26 41839.0 27 50129.0 28 61212.0 29 74505.0 30 90763.0 31 112620.0 32 141329.0 33 186837.0 34 260426.0 35 389584.0 36 598139.0 37 796057.0 38 456622.0 39 26613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71565748875196 18.07239178055749 14.635050192714916 23.576900537975636 2 16.084806756129964 19.30856649908548 39.23045413975867 25.376172605025886 3 19.904306881745708 22.78425488664099 29.281817616107418 28.02962061550588 4 13.524423099579034 16.029980403555125 35.097437417760474 35.348159079105365 5 14.335876874607987 36.540157015184924 33.68561453734503 15.438351572862057 6 34.154277149083505 34.853372271817165 16.18306065332581 14.809289925773514 7 29.939659227707804 32.128426092855484 19.301193070836177 18.63072160860053 8 27.461710051156512 34.32430982405236 18.61797773317308 19.596002391618054 9 26.481797518392746 16.50793993190749 19.404595677555328 37.60566687214443 10-11 25.83838662065956 25.837460173126768 26.1736361793525 22.150517026861174 12-13 26.642764320320754 23.0757477330382 26.89182660331425 23.389661343326797 14-15 23.144221885013756 24.518503093090278 24.977219069996014 27.360055951899948 16-17 22.91471179323461 27.782695785327448 26.023095369062126 23.279497052375813 18-19 23.100277851294386 25.982248712168794 28.34008533826259 22.577388098274238 20-21 23.31799302149939 25.132599531577064 28.863680297613666 22.68572714930988 22-23 23.20395700951586 25.00578684018614 28.858605577546008 22.931650572751987 24-25 23.071333445891803 25.324549347831528 28.69969939698252 22.90441780929415 26-27 22.546607113862237 25.678039216445605 28.627792298391746 23.147561371300412 28-29 22.815392952370654 26.02971802596466 28.43164327196132 22.723245749703363 30-31 22.767131997155392 25.5880698918033 28.37749472097386 23.267303390067454 32-33 22.53022302640267 25.582650167907623 28.441580799969024 23.445546005720683 34-35 23.231012891059127 25.676001984856896 28.272400033855416 22.820585090228562 36-37 23.270818409865946 25.26714274256224 28.277207267256138 23.18483158031568 38-39 22.85806673615122 25.660352202593945 28.19736821723438 23.284212844020455 40-41 23.27529321685385 25.50637054510998 28.311993990785076 22.90634224725109 42-43 22.965724083812063 25.535567241145152 28.08308846979721 23.41562020524557 44-45 22.95102302607287 25.766616135592546 28.31117516692264 22.97118567141194 46-47 23.450084453795167 26.030118511870892 27.570904198352686 22.94889283598126 48-49 23.14532821398709 26.160381077593165 27.375066597178368 23.319224111241383 50-51 23.167549004234832 26.325324678377516 27.458245562800283 23.048880754587366 52-53 23.580219156003533 26.009931517551475 27.346975065840002 23.06287426060499 54-55 23.172858793079463 26.186302238158966 27.443934022554988 23.196904946206583 56-57 23.107163983702268 26.44789230420776 27.275445019763755 23.169498692326222 58-59 23.070410289043334 26.055576343010145 27.245771043564325 23.62824232438219 60-61 22.73697214269062 26.308565657336647 27.52609747389561 23.42836472607712 62-63 22.885162265211232 26.280896679529075 27.686386724642926 23.147554330616767 64-65 22.590869984012556 26.410225989591158 27.511384933971943 23.487519092424343 66-67 22.60251374879216 26.393546488699798 27.416457731486627 23.587482031021413 68-69 23.04788193001189 25.982457755996546 27.656025776855202 23.313634537136366 70-71 22.95345665871028 26.15882684640302 27.164699970657885 23.723016524228814 72-73 22.879064304584976 26.288792224920442 27.091012822863508 23.741130647631074 74-75 22.70606751230585 26.754103955156623 26.988937663355518 23.550890869182016 76-77 22.894966015968638 26.294793392520887 27.215723722836767 23.59451686867371 78-79 22.8993631848556 26.47827148673811 27.22732505835928 23.39504027004701 80-81 23.067160791342484 26.506845422504394 26.95768049200813 23.468313294144995 82-83 22.948741207646396 26.53256731470722 27.060302119014608 23.458389358631788 84-85 22.72621739321022 26.72553373284505 26.991627511300653 23.556621362644073 86-87 23.044717341434513 26.64551492214324 26.76686666104341 23.542901075378843 88-89 22.859123757142292 26.737470343291864 26.544534080981514 23.858871818584326 90-91 22.72436192168481 26.695599567181876 26.735161632678462 23.844876878454855 92-93 22.865941978384352 26.338546861394896 26.91491063678919 23.88060052343156 94-95 22.607065679413314 26.18155409990935 27.0347028367033 24.17667738397404 96-97 22.901038010992398 25.84857274179926 27.03778864443519 24.212600602773154 98-99 22.571359710018797 26.30180905007621 26.68640970298746 24.44042153691753 100-101 22.861954617228317 25.700711327799446 26.942620719065967 24.49471333590627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1451.0 1 1008.5 2 796.0 3 1061.5 4 853.0 5 781.0 6 1619.0 7 3667.0 8 6014.5 9 6882.0 10 5669.0 11 4133.0 12 3742.0 13 3887.5 14 4082.5 15 4171.5 16 4301.0 17 4527.5 18 4873.5 19 5272.0 20 5743.0 21 6714.0 22 8066.0 23 9812.0 24 12125.5 25 14934.0 26 18271.0 27 22195.0 28 25375.5 29 28429.0 30 32715.0 31 39417.5 32 49448.5 33 53245.0 34 52051.0 35 54516.0 36 59597.5 37 66041.0 38 73190.5 39 78787.0 40 84649.0 41 93595.0 42 98791.5 43 102676.5 44 109145.0 45 117846.0 46 123689.5 47 129205.5 48 135414.5 49 136493.5 50 138170.5 51 140188.0 52 142553.0 53 147672.0 54 151047.5 55 143338.0 56 132964.5 57 124547.0 58 116397.0 59 103663.0 60 83240.0 61 67915.5 62 56698.0 63 42462.5 64 30024.0 65 22629.5 66 16747.5 67 12589.5 68 9536.0 69 7055.0 70 5158.5 71 3472.0 72 2315.0 73 1720.5 74 1264.0 75 887.5 76 608.5 77 373.0 78 220.5 79 119.0 80 56.5 81 33.5 82 21.5 83 10.5 84 6.0 85 5.0 86 2.0 87 1.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011117370393447058 3 0.0065819257553243775 4 2.7655150232455367E-5 5 1.9358605162718754E-4 6 8.29654506973661E-5 7 0.0 8 2.7655150232455367E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001783757189993371 26-27 0.02201349958503447 28-29 2.62723927208326E-4 30-31 6.913787558113842E-5 32-33 0.003747272856497702 34-35 0.016288883486916213 36-37 0.008102959018109422 38-39 0.021322120829223086 40-41 0.023465394972238377 42-43 0.01891612275899947 44-45 0.010121784985078663 46-47 0.002226239593712657 48-49 1.7975847651095986E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.488963520920983E-4 68-69 1.659309013947322E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7975847651095986E-4 82-83 0.0010232405586008484 84-85 0.0036643074058003355 86-87 7.743442065087501E-4 88-89 5.5310300464910735E-5 90-91 0.0034707213541731482 92-93 4.8396512906796887E-4 94-95 1.3827575116227684E-4 96-97 2.488963520920983E-4 98-99 1.2444817604604914E-4 100-101 0.003304790452778416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3615963.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.00273712169137 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15295492335467 30.429192966805484 2 15.551537119535382 13.686203994182817 3 5.908039976088282 7.799097899167694 4 2.8710091194219878 5.053290382236176 5 1.6520505957643612 3.63473740385764 6 1.052414664712637 2.7785475500617878 7 0.7016731519744549 2.1612877476176378 8 0.519792605536363 1.8297837903372478 9 0.3776711892157245 1.495670945174648 >10 1.9940325668785133 15.837918249808908 >50 0.1378236906395025 4.194230139884687 >100 0.07293556480835049 5.702478145815205 >500 0.004410455046061759 1.315975143477662 >1k 0.0034653575225497917 3.0564413960417154 >5k 6.300650040999621E-5 0.23352417730703418 >10k+ 1.2601300081999242E-4 0.7916200682236432 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15609 0.4316692399783958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12944 0.35796826460890224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8423 0.23293933040797155 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4672 0.12920486188603147 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4593 0.1270201050176675 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4214 0.1165388030795669 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4118 0.11388390865725119 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3838 0.1061404665921637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.29654506973661E-5 2 0.0 0.0 0.0 0.0 8.29654506973661E-5 3 2.7655150232455364E-5 0.0 0.0 0.0 8.29654506973661E-5 4 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 8.29654506973661E-5 5 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 8.29654506973661E-5 6 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 1.1062060092982146E-4 7 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 1.3827575116227682E-4 8 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 1.3827575116227682E-4 9 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 1.659309013947322E-4 10-11 2.7655150232455364E-5 0.0 0.0 2.7655150232455364E-5 1.659309013947322E-4 12-13 2.7655150232455364E-5 0.0 0.0 5.531030046491073E-5 7.052063309276118E-4 14-15 2.7655150232455364E-5 0.0 0.0 5.531030046491073E-5 8.573096572061162E-4 16-17 2.7655150232455364E-5 0.0 0.0 6.913787558113841E-5 9.402751079034824E-4 18-19 2.7655150232455364E-5 0.0 0.0 9.679302581359377E-5 9.402751079034824E-4 20-21 2.7655150232455364E-5 0.0 0.0 1.521033262785045E-4 0.0010370681337170762 22-23 2.7655150232455364E-5 0.0 0.0 2.488963520920983E-4 0.0011338611595306698 24-25 5.531030046491073E-5 0.0 0.0 5.392754295328796E-4 0.0011891714599955806 26-27 5.531030046491073E-5 0.0 0.0 9.955854083683931E-4 0.0012721369106929467 28-29 5.531030046491073E-5 0.0 0.0 0.0037334452813814747 0.0013136196360416298 30-31 5.531030046491073E-5 0.0 0.0 0.01234802457879132 0.0013274472111578575 32-33 5.531030046491073E-5 0.0 0.0 0.030683389182909228 0.0013274472111578575 34-35 5.531030046491073E-5 0.0 0.0 0.05966598662652245 0.0014104126618552236 36-37 5.531030046491073E-5 0.0 0.0 0.10318136551729097 0.0014104126618552236 38-39 8.29654506973661E-5 0.0 0.0 0.16913889882169703 0.0014657229623201344 40-41 8.29654506973661E-5 0.0 0.0 0.23750243019632666 0.0014657229623201344 42-43 8.29654506973661E-5 0.0 0.0 0.3047044452611932 0.0015072056876688175 44-45 8.29654506973661E-5 0.0 0.0 0.38968872192552856 0.001562515988133728 46-47 8.29654506973661E-5 0.0 0.0 0.4894685039642275 0.0016454814388310941 48-49 1.1062060092982146E-4 0.0 0.0 0.5919031804252421 0.0017975847651095988 50-51 1.1062060092982146E-4 0.0 0.0 0.7007953344655351 0.0018667226406907371 52-53 1.1062060092982146E-4 0.0 0.0 0.8043638720860805 0.0020741362674341524 54-55 1.1062060092982146E-4 0.0 0.0 0.9177499880391475 0.002129446567899063 56-57 1.1062060092982146E-4 0.0 0.0 1.0462358160191352 0.0021985844434802013 58-59 1.1062060092982146E-4 0.0 0.0 1.1819672933600263 0.002212412018596429 60-61 1.3827575116227682E-4 0.0 0.0 1.3227458356183401 0.0022538947439451123 62-63 1.3827575116227682E-4 0.0 0.0 1.4627085509447968 0.0024336532204560723 64-65 1.3827575116227682E-4 0.0 0.0 1.6093499850523911 0.0024336532204560723 66-67 1.3827575116227682E-4 0.0 0.0 1.7783782632731584 0.0024474807955723 68-69 1.3827575116227682E-4 0.0 0.0 1.9637922180066556 0.0024613083706885273 70-71 1.3827575116227682E-4 0.0 0.0 2.1516259983854926 0.0025304462462696657 72-73 1.3827575116227682E-4 0.0 0.0 2.346014049369421 0.0027931701734779917 74-75 1.3827575116227682E-4 0.0 0.0 2.5413285478861374 0.0029452734997564966 76-77 1.3827575116227682E-4 0.0 0.0 2.7507056902960567 0.00304206652557009 78-79 1.3827575116227682E-4 0.0 0.0 2.979441437868695 0.003138859551383684 80-81 1.3827575116227682E-4 0.0 0.0 3.2241618622756922 0.0032079974269648226 82-83 1.659309013947322E-4 0.0 0.0 3.479819898599626 0.00340158347859201 84-85 1.797584765109599E-4 0.0 0.0 3.7425853085333007 0.003429238628824465 86-87 1.9358605162718756E-4 0.0 0.0 4.008987370722544 0.0036089971053354254 88-89 1.9358605162718756E-4 0.0 0.0 4.292978108459628 0.0037334452813814743 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7135 0.0 54.39702 1 GTATCAA 12310 0.0 40.829556 1 TATCAAC 15375 0.0 32.50481 2 ATCAACG 15490 0.0 32.10615 3 TCAACGC 15815 0.0 31.593044 4 CAACGCA 15970 0.0 31.286411 5 AACGCAG 16635 0.0 30.064259 6 ACGCAGA 18985 0.0 26.192745 7 CGCAGAG 19055 0.0 25.996824 8 TAAGGTG 2365 0.0 24.70125 5 GTAAGGT 2245 0.0 23.694448 4 TGGTATC 3040 0.0 23.440384 2 GTACATG 12480 0.0 22.57289 1 GCAGAGT 21710 0.0 22.226896 9 AGGTAAG 2100 0.0 22.169394 2 TACATGG 12505 0.0 21.995909 2 GAGTACT 13265 0.0 21.966063 12-13 GTGGTAT 3485 0.0 21.674114 1 CAGAGTA 20025 0.0 20.990156 10-11 ACATGGG 12435 0.0 20.97102 3 >>END_MODULE