FastQCFastQC Report
Thu 26 May 2016
SRR938173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632168
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162200.44656524698196776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.34356890980813665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81280.22377819528171605No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46440.12785752200889386No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45760.1259853619105724No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42070.11582614020056341No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39640.10913592102567943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71850.053.828921
GTATCAA123900.040.04071
TATCAAC154400.031.7949522
ATCAACG156600.031.1661993
TCAACGC157500.030.9881084
CAACGCA160400.030.398225
AACGCAG166200.029.3659886
TGGTATC33050.026.7452492
TAAGGTG23050.025.771775
GTGGTAT36550.025.6050781
ACGCAGA190250.025.5158377
CGCAGAG191250.025.3323528
GTGTAAG14000.023.8741231
AGGTAAG21150.023.1436582
GTACATG123750.022.5333141
GAGTACT129300.022.38946312-13
GTAAGGT23600.022.351994
GCAGAGT215450.022.1329469
TACATGG123900.022.131442
AAGGTAA21850.021.8527451
Produced by FastQC (version 0.11.5)